ライフサイエンスコーパス: cross-linking

1 to address a larger part of the proteome by cross-linking.

2 eases the oxygen availability during corneal cross-linking.

3 PP), c) protein footprinting, and d) protein cross-linking.

4 rthritis, and following IgG Fcgamma receptor cross-linking.

5 and 1627 cm(-1) increased with increasing GA cross-linking.

6 intermediates, and investigate them by thiol cross-linking.

7 icated by in vivo and in vitro site-directed cross-linking.

8 and state-dependent non-canonical amino acid cross-linking.

9 meshwork due to its dendritic structure and cross-linking.

10 obase targeting from recognition to covalent cross-linking.

11 oxygen allowing a potentially more efficient cross-linking.

12 that enable facile and cytocompatible photo-cross-linking.

13 ,2'-(ethylenedioxy) diethanethiol (EDT)) for cross-linking.

14 ations in the degree of iron-induced protein cross-linking.

15 iosynthetic explanation for colibactin's DNA cross-linking activity and paves the way for further stu

16 How cells regulate microtubule cross-linking activity to control the rate and duration

17 Using a combination of cross-linking/affinity pull-downs and two-hybrid assays,

18 d by higher protein cross-linking and higher cross-linking AGE levels.

19 This suggests that OSA could be acting as a cross-linking agent between starch chains, reducing the

20 kout cells to a low concentration of the DNA cross-linking agent mitomycin C (MMC).

21 sodium alginate using transglutaminase as a cross-linking agent.

22 C-MS/MS analyses revealed that the levels of cross-linking AGEs were significantly higher in the glyc

23 s directly correlated with levels of protein-cross-linking AGEs.

24 drive the symptoms of allergic disease upon cross-linking allergens on mast cells or basophils.

25 However, quantitative MS and cross-linking analyses revealed that despite having almo

26 ometry workflow for performing proteome-wide cross-linking analyses within 1 week.

27 in live mycobacteria, enabling in vivo photo-cross-linking and click-chemistry-mediated analysis of m

28 an imaging could rapidly identify degrees of cross-linking and detected calcified regions with high c

29 Inhibiting LOX reduces collagen cross-linking and fibronectin assembly, increases drug p

30 this loss was accompanied by higher protein cross-linking and higher cross-linking AGE levels.

31 way, supplemented with results from chemical cross-linking and hydrogen-deuterium exchange MS, reveal

32 on of Endogenous Argonaute-bound RNAs)-CLIP (Cross-Linking and Immuno-Precipitation) to explore the r

33 of RNA binding proteins (RBP) motifs and UV-cross-linking and immunoprecipitation (CLIP) assays iden

34 AGO cross-linking and immunoprecipitation (CLIP) experiments

35 ing photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation analysis, we found

36 this, we performed enhanced ultraviolet (UV) cross-linking and immunoprecipitation followed by sequen

37 Enhanced UV-cross-linking and immunoprecipitation of these RBPs iden

38 sed photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation to demonstrate tha

39 Cross-linking and immunoprecipitation, followed by high-

40 tended the target validation method chemical cross-linking and isolation by pull-down (Chem-CLIP) to

41 y target tau pre-mRNA in cells, via chemical cross-linking and isolation by pull-down target profilin

42 this review is on the fundamentals of photo-cross-linking and key criteria identified for the succes

43 ibroblast polarization; elastomers with high cross-linking and low deformability were required for po

44 rom resolution, in conjunction with chemical cross-linking and mass spectrometric analysis of conform

45 dent manner, providing a mechanism for HC.HA cross-linking and matrix stabilization.

46 Furthermore, cross-linking and mutagenesis studies indicated that thi

47 Here, we utilized structure-guided cysteine cross-linking and proteolysis-coupled gel analysis to pr

48 ercially available biotin-benzophenone photo-cross-linking and purification protocol.

49 ially inhibit XCTK2 antiparallel microtubule cross-linking and sliding by decreasing the microtubule

50 We developed viral cross-linking and solid-phase purification (VIR-CLASP) t

51 We established VIR-CLASP (VIRal Cross-Linking And Solid-phase Purification) to identify

52 een MurJ and its substrate Lipid II by photo-cross-linking and subsequent biotin-tagging.

53 orks by using data from structural, chemical cross-linking and various high-throughput studies.

54 chemical modifications, e.g., (succinylation/cross-linking) and two enzymes modification (alpha-amyla

55 e functionalization, polymer conjugation and cross-linking, and biomolecular labeling in the native c

56 all-angle x-ray scattering, targeted protein cross-linking, and cryo-electron microscopy.

57 ectron-transfer dissociation (ETD), chemical cross-linking, and molecular docking.

58 erimental factors, such as antibody quality, cross-linking, and PCR biases, are known to affect the o

59 regulate glycan strand length, the degree of cross-linking, and the addition of other modifications t

60 imal keratometry (K(max)) at 12 months after cross-linking, and we considered additional topographic,

61 g whether transepithelial and epithelium-off cross-linking are comparable in their safety and efficac

62 nstrated in vitro, the in vivo mechanisms of cross-linking are not well-established.

63 llowing a straightforward synthetic route by cross-linking arylamine-based ligands with a simple thie

64 ified IAA response and lignin synthesis/wall cross-linking as some of the key processes occurring dur

65 was shown to influence network topology upon cross-linking, as evidenced by sequence-dependent rubber

66 The cross-linking behavior of PSBBB was investigated by Four

67 ccinimide or isopeptide bond moieties due to cross-linking between Asn or Gln and Lys side chains.

68 ic unit extended farther away from the core, cross-linking between neighboring columns was suggested

69 Cross-linking between the C-terminal region of both E1a

70 whose structure and dynamics are modified by cross-linking, bridging proteins, and cleavage by matrix

71 in reconstituted and native chromatin after cross-linking by ultraviolet (UV) light.

72 ow allows for application of a wide range of cross-linking chemistries and enables parallel compariso

73 od development, combining several orthogonal cross-linking chemistries as well as improvements in sea

74 We then investigate the cross-linking chemistries of two poorly characterized re

75 the case of EMCS, we find that the expected cross-linking chemistry is accompanied by a competing ch

76 hils are activated in the presence of CD300c cross-linking compared with activation through the IgE/F

77 Here, we combine in vitro biochemistry, cross-linking coupled to mass spectrometry, and in vivo

78 Chemical cross-linking coupled with mass spectrometry offers an a

79 lagen is a structural protein whose internal cross-linking critically determines the properties and f

80 g., free radical chain polymerization, click cross-linking, cross-linking due to oxidation of phenoli

81 Cysteine-cross-linking, cryo-electron microscopy multivariate ana

82 elerated (9 mW/cm(2) for 10 minutes) corneal cross-linking (CXL).

83 ling was linearly dependent on the degree of cross-linking (DC), and higher water flux was obtained i

84 This study reveals that cross-linking decreases NP diffusion coefficients and po

85 he linker concentration still determines the cross-linking degree of the vitrimers, which originates

86 on nano-textured non-wovens encoded by their cross-linking degree.

87 We further demonstrate that this level of cross-linking density is sufficient to model the archite

88 2 mJ/kg was obtained by a hydrogel with a 3% cross-linking density, a 200-300 mum particle size, and

89 The cross-linking density, external load, particle size, and

90 By varying polymer concentration or cross-linking density, the elastic moduli of centimeter-

91 by 92% from 407 to 31 g/g because of higher cross-linking density.

92 mutant CTxB-single-GM1 complexes by antibody cross-linking does not rescue the membrane curvature phe

93 X toxin with three effector domains-an actin cross-linking domain (ACD), a Rho inactivation domain (R

94 l chain polymerization, click cross-linking, cross-linking due to oxidation of phenolic groups), (ii)

95 discussed in detail, including (i) covalent cross-linking (e.g., free radical chain polymerization,

96 The general mechanisms associated with photo-cross-linking (e.g., free-radical chain polymerization,

97 e Diels-Alder reactions), and (iii) physical cross-linking (e.g., guest-host interactions, hydrogen b

98 n of phenolic groups), (ii) dynamic covalent cross-linking (e.g., Schiff base formation, disulfide fo

99 Due to the cross-linking effect of glutaraldehyde, anti-leptin can

100 benzylic leaving groups greatly affected DNA cross-linking efficiency.

101 These results indicate that dynamic actin cross-linking enables the cytoplasm to flow at long time

102 Lysyl oxidase (LOX), a collagen cross-linking enzyme, has not been investigated in the c

103 ds at the interface as a result of oxidative cross-linking, even though the modulus of the bulk of th

104 level information all from a single in vivo cross-linking experiment.

105 ward-facing conformation and verify it using cross-linking experiments.

106 Here, mechanistic cross-linking facilitates the structural characterizatio

107 ndent manner and show that microtubule actin cross linking factor 1 (MACF1), a scaffolding protein wi

108 comparing transepithelial and epithelium-off cross-linking for corneal ectasia.

109 ional changes associated with gating promote cross-linking for I409C/R410C, which in turn decreases c

110 We excluded studies evaluating cross-linking for nonectatic indications, as well as non

111 ention of key 4HB residues to participate in cross-linking for oligomer formation.

112 e in SikuliX to coordinate image capture and cross-linking functions in Zeiss Zen Black with image pr

113 nd chemical conjugation methods to introduce cross-linking groups are described.

114 d between transepithelial and epithelium-off cross-linking groups in the change in K(max) at 12 month

115 eptor coactivators, and induction of protein cross-linking have also been described.

116 Although multiple mechanisms of apoA-I cross-linking have been demonstrated in vitro, the in vi

117 uvate kinase muscle isozyme M2 and after KIR cross-linking have increased phosphorylation of the meta

118 Here, we use chemical cross-linking, hydrogen-deuterium exchange mass spectrom

119 articularly with regards to the use of photo-cross-linking (i.e., light-based) techniques.

120 Furthermore, cross-linking identifies another loop region (i.e., the

121 Structure guided mutagenesis, cross-linking immunoprecipitation sequencing assays, and

122 ment, chromatin immunoprecipitation-seq, and cross-linking immunoprecipitation-seq showed that the KH

123 We further demonstrate that cross-linking impairs the cholesterol efflux mediated by

124 fety and efficacy of transepithelial corneal cross-linking in comparison with the established epithel

125 While the role of actin cross-linking in controlling actin network mechanics is

126 including mechanochromism and force-induced cross-linking in ferrocenophane-containing polymers.

127 lysis, we analyzed the pattern of apoprotein cross-linking in isolated human HDL either by synthetic

128 m these mice, indicate that overall collagen cross-linking in mouse tendon decreases with age.

129 e data indicate a critical role for collagen cross-linking in OSCC tumor progression and metastasis,

130 We detected a similar pattern of apoprotein cross-linking in oxidized murine HDL.

131 C57BL/6 mice, the overall levels of collagen cross-linking in tail tendon decreased with age.

132 rmation of a tetramer was further studied by cross-linking in the presence of detergents and lipids.

133 urrent scientific consensus is that collagen cross-linking increases with age and that this increase

134 The protein cross-linking induced by kansui (1.0%) improved the text

135 A cross-linking-informed high-resolution model of the CXCR

136 mation and is categorized as a new adipokine cross-linking innate immunity and metabolic disorders in

137 h-quality transcriptome-wide in vivo RBP-RNA cross-linking interaction datasets generated by the same

138 y engineering, defect passivation, molecular cross-linking, interfacial energy alignment and electron

139 biochemical analyses to examine if collagen cross-linking is associated with tumor stage and regiona

140 Development of new reagents for protein cross-linking is constantly ongoing.

141 nerated by Cin8 and Kip3 through microtubule cross-linking is essential for signaling efficient cohes

142 seen when actin assembly or disassembly and cross-linking is experimentally disrupted in fibroblasts

143 e mechanism that has been implicated in this cross-linking is glycation through formation of advanced

144 integrin alphaLbeta2 (LFA-1) on T cells, and cross-linking layilin promoted the activated state of th

145 MT cytoskeleton in axons and dendrites: the cross-linking level of MTs by motors and the load acting

146 uantitatively characterize the dependence of cross-linking lifetime of actin cross-linkers on mechani

147 es to nutrient availability, we performed UV cross-linking, ligation and sequencing of hybrids (CLASH

148 ment in type III peroxidase-mediated polymer cross-linking, lignification and, possibly, cell expansi

149 the limited actions of three kinesins and a cross-linking MAP that are known to have effects in main

150 e used an integrated combination of chemical cross-linking mass spectrometry (CXMS), molecular geneti

151 We have taken advantage of cross-linking mass spectrometry (XL-MS) and molecular do

152 ng question is to understand how large-scale cross-linking mass spectrometry (XL-MS) can confirm and

153 Cross-linking mass spectrometry (XL-MS) has become a pow

154 vel interactions identified by proteome-wide cross-linking mass spectrometry (XL-MS) studies is criti

155 To illuminate these dark areas, we applied cross-linking mass spectrometry (XL-MS) to obtain bioche

156 In cross-linking mass spectrometry (XL-MS), the depth and s

157 Here, we have developed a multichemistry cross-linking mass spectrometry approach enabled by thre

158 Finally, cross-linking mass spectrometry reveals likely interacti

159 Analysis of the CMG-Mcm10 complex by cross-linking mass spectrometry reveals Mcm10 interacts

160 ffinity purification, live cell imaging, and cross-linking mass spectrometry to build integrative str

161 By coupling SCAP to cross-linking mass spectrometry, an integrative structur

162 ted using cryoelectron microscopy (cryo-EM), cross-linking mass spectrometry, and homology modeling.

163 Mycoplasma pneumoniae We combined whole-cell cross-linking mass spectrometry, cellular cryo-electron

164 We use cross-linking mass spectrometry, recombinant protein and

165 e determine its topology in the replisome by cross-linking mass spectrometry.

166 n nuclear magnetic resonance (NMR), chemical cross-linking, mass spectrometry, and molecular docking.

167 We present a cross-linking/mass spectrometry workflow for performing

168 These results suggest that FXIIIa-mediated cross-linking may contribute to the formation of Abeta d

169 These results uncover a protein cross-linking mechanism in the lens and suggest that AGE

170 oactivity of these natural products to a DNA cross-linking mechanism, but this hypothesis was not con

171 le hydrogels, obtained by employing distinct cross-linking mechanisms, to assess their potential for

172 The application of all cross-linking methods increased the resistance of rabbit

173 All cross-linking methods reduced the enzymatic degradation

174 Cross-linking methods to form biopolymer networks are th

175 sis on biopolymer chemical modifications and cross-linking methods.

176 Additionally, cross-linking might inhibit degrading processes in idiop

177 active-site-directed photoprobe with double cross-linking moieties, we demonstrate that GSAP modifie

178 New thiophene-carbazole functional and cross-linking monomers electropolymerizing at potentials

179 designed and synthesized new functional and cross-linking monomers, which are oxidized at sufficient

180 -insoluble digestion method and quantitative cross-linking MS, we identified the previously mapped fi

181 ers with appropriate side chains, backbones, cross-linking networks, and flexible blocks, and (3) emp

182 Here, we demonstrate that chemical cross-linking of a fibrous matrix of synthetic semiflexi

183 sm in the lens and suggest that AGE-mediated cross-linking of alpha-crystallin-client complexes could

184 es is therefore related to a peptide-induced cross-linking of bilayers.

185 olution pH, which promoted oxidation-induced cross-linking of catechol and subsequently decreased its

186 ngagement of both the BCR and CD40 or strong cross-linking of CD40 alone.

187 Knowing how the cross-linking of collagen changes with age is key to und

188 Mast cell and basophil activation by antigen cross-linking of FcepsilonRI-bound IgE is central to all

189 the chains are kinked, enabling non-covalent cross-linking of fibrils and disfavoring formation of pa

190 life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the sur

191 Cross-linking of high-affinity immunoglobulin E (IgE) re

192 Ab cross-linking of HLA class I (HLA I) molecules on the su

193 phylaxis is classically mediated by allergen cross-linking of IgE bound to the alpha chain of Fcepsil

194 is not caused by classical, antigen-induced cross-linking of IgE receptors.

195 By UV cross-linking of isolated nuclei we determined the cross

196 C, which in turn decreases channel currents, cross-linking of L402C/L403C is functionally silent in m

197 te with maize psbA mRNA by: (i) formaldehyde cross-linking of leaf tissue followed by antisense oligo

198 Oxidative cross-linking of matrix fibronectin attenuated both epit

199 Taken together, our work reveals that cross-linking of microtubules by MAP65-1 confers resista

200 A, reconstituted in nanodiscs, with cysteine cross-linking of natively expressed membrane-embedded Md

201 cule, which we denote RIPR-PD1, that induces cross-linking of PD-1 to CD45 and inhibits both tonic an

202 Here we demonstrate that glutaraldehyde cross-linking of PEGylated oligolysine-coated DNs extend

203 Cross-linking of Pgamma to GAFab in conjunction with sol

204 to recognize epitopes formed by the covalent cross-linking of proinsulin and secretory granule peptid

205 ntinuous tuning of RBC strains, and chemical cross-linking of RBCs, a model for diseased cells, leads

206 FT-IR had confirmed the cross-linking of succinic anhydride as indicated by the

207 ore effective at intermolecular, noncovalent cross-linking of tau.

208 nm thick carbon nanomembrane (CNM) made from cross-linking of terphenylthiol (TPT) self-assembled mon

209 aviors change as a result of calcium-induced cross-linking of the alginate matrix.

210 ccurs via multiple mechanisms, which include cross-linking of the IgE-bound, high-affinity IgE recept

211 Allergen cross-linking of the IgE/FcepsilonRI complex activates t

212 results in topological [2 + 2] cycloaddition cross-linking of the pai-stacked layers in 3D COFs.

213 erential scanning calorimetry, demonstrating cross-linking of the polymer after curing at 120 degrees

214 Collagen cross-linking of the sclera is a promising approach to s

215 d consequent inactivation through C-terminal cross-linking of the tear mitogen lacritin, leading to s

216 Finally, Siglec-7 cross-linking on GM-CSF-activated eosinophils induced ph

217 d by transglutaminase-2, but the function of cross-linking on their elastic properties is yet to be e

218 propose that increased signaling by FcgammaR cross-linking on TLR-stimulated macrophages can paradoxi

219 ed with excessive myocardial collagen type-I cross-linking or CCL+ (i.e., decreased carboxy-terminal

220 fabrication, achieved by oxidative covalent cross-linking or formation of metal coordination interac

221 ia acid-triggered coloration, polymerization/cross-linking, or degradation.

222 identified at least two distinct DOPA-based cross-linking pathways during byssus fabrication, achiev

223 al peptide synthesis with oxidative aromatic cross-linking performed by cytochrome P450 enzymes.

224 ones with naphthalenes as a dynamic covalent cross-linking platform that enables green light-induced

225 nd activate T cells with solution ligands by cross-linking pMHC or using multivalent antibodies to TC

226 UV irradiation of the films resulted in cross-linking/polymerization of the molecular columns.

227 Bionic nanocapsules are designed by cross-linking polythymine and photoisomerized polyazoben

228 earts showed reduced collagen expression and cross-linking post-AB.

229 rminal d-ala-d-ala and reduced peptidoglycan cross-linking, prompting us to investigate synergism bet

230 dual residues in order to assess the spatial cross-linking propensity of detected pairs.

231 ity, swelling power, viscosity and degree of cross-linking properties.

232 Cross-linking, protein capture and two-hybrid studies de

233 d morphology, targeting vimentin or vimentin cross-linking proteins could provide a therapeutic appro

234 al conditions in the absence of any specific cross-linking proteins, which suggests that it is driven

235 Chemical cross-linking provides complementary information on one

236 We observe a clear dependency of cross-linking rates on the electrophilicity.

237 with a 2-methoxy substituent showed a faster cross-linking reaction rate and higher ICL efficiency th

238 ins within those structures by a photodriven cross-linking reaction so that they can be affinity puri

239 a complementary approach, we utilized HaXS8 cross-linking reactions between Halo and SNAP proteins a

240 ebrates, and there is evidence for oxidative cross-linking reactions occurring in extracellular prote

241 ed with conventional methods using redundant cross-linking reactions, our method is simpler, faster,

242 oyed a normalization strategy to dissect the cross-linking reactivity of individual residues in order

243 In addition, the impact on cross-linking reactivity was evaluated.

244 y adding either supplemental TNF ligand or a cross-linking reagent, suggesting that the antibody agon

245 Two DNA-cross-linking reagents, bis-chloroethylnitrosourea and 8

246 Since these organic cross-linking reagents, in comparison with inorganic app

247 The efficacy of transepithelial cross-linking remains inferior to the epithelium-off app

248 amages neurons and the myocardium by protein cross-linking, resulting in conglomerations and cell dea

249 f the PpiD-SecY interaction by site-directed cross-linking revealed that PpiD and the established Sec

250 Photochemical cross-linking reveals that the baboon amylin, like human

251 oelastin molecule to regions remote from the cross-linking site.

252 linking of isolated nuclei we determined the cross-linking sites of several factors including chromat

253 microbial efficacy, in vitro, of 5 candidate cross-linking solutions against 5 different microbial pa

254 multi-subunit ubiquitin ligase and chemical cross-linking steps.

255 a laboratory artifact rather than a natural cross-linking structure.

256 a major limitation in current proteome-wide cross-linking studies and demonstrates how to address a

257 Proteome-wide cross-linking studies have spurred great interest as the

258 In vivo and in vitro cross-linking studies provide further support for these

259 Mutational and cross-linking studies show that the dimer-dimer interfac

260 f point mutants in both AimB and MreB, photo-cross-linking studies with site-specific unnatural amino

261 on native tissues, as well as peptide-based cross-linking studies, we have identified at least two d

262 G (4) and related structures, as well as DNA cross-linking studies.

263 Cross-linking sucrose density gradient centrifugation de

264 Here we show that transglutaminase cross-linking supports formation of a 1:1 stoichiometric

265 he antibody bound to TNFR2 through a natural cross-linking surface and that T(reg) cell expansion was

266 A cross-linking technique involving application of Bacteri

267 lient complexes underwent greater extents of cross-linking than did uncomplexed protein mixtures.

268 a suggest a long-range stabilising effect of cross-linking that occurs due to the covalent linkage of

269 challenge and chemically or photochemically cross-linking the cornea for therapy presents a unique a

270 The latter has been implicated in cross-linking the protofilaments.

271 Using functional assays, we found that cross-linking the sigma1 receptor-binding domain modulat

272 aphene nanosheets (GNSs) and following photo-cross-linking, the resultant nanostructured HRP@PGH/GNSs

273 oss-linker alpha-actinin-4 and show that the cross-linking time of alpha-actinin-4 homodimers increas

274 s unique arginine (Arg59) and simultaneously cross-linking to another arginine (Arg45) located across

275 of TM2 in TM signaling, we use oxidative Cys cross-linking to demonstrate that TM2 extends over the f

276 roscopy, molecular dynamics, and biochemical cross-linking to determine a 2.3 angstrom structure, and

277 We use site-specific cross-linking to engineer ClpA hexamers with alternating

278 l to promote Kinesin-14 parallel microtubule cross-linking to help focus spindle poles for efficient

279 Global analysis of BUD13 knockdown and BUD13 cross-linking to RNA revealed a subset of introns that s

280 tosis, are mediated by FcgammaRs, which upon cross-linking transduce signals that modulate the functi

281 Different cross-linking treatments were tested to increase resista

282 underwent multimerization by o,o'-dityrosine cross-linking under reducing conditions that disrupt dis

283 OCTs of 25 eyes of 25 patients post corneal cross-linking using the Dresden protocol, at 3 months po

284 Disulfide cross-linking validates the physiological relevance of t

285 ects the relative contribution of ensembles' cross-linking versus motoring activities.

286 Transepithelial cross-linking was associated with significantly fewer co

287 l stiffness decreased when active or passive cross-linking was reduced and softer cells polarized on

288 By comprehensive disulfide cross-linking, we establish the existence and the struct

289 Using in vivo cross-linking, we found that the extracellular juxtamemb

290 se functions through SEPT9-dependent F-actin cross-linking, which enables the generation of F-actin b

291 with trimethylsilyl (TMS) groups followed by cross-linking with a bis-silyl ether cross-linker.

292 Here, intact yeast nuclei were subject to cross-linking with disuccinimidyl sulfoxide (DSSO) and a

293 Chemical cross-linking with mass spectrometry (XL-MS) has emerged

294 scribes a workflow for utilizing large-scale cross-linking with mass spectrometry (XL-MS) to make sys

295 Here we utilize chemical cross-linking with mass spectrometry to identify protein

296 Cross-linking with reagents that differ in spacer length

297 Especially, gentle cross-linking with riboflavin and UV-A or blue light mig

298 Cross-linking with riboflavin/blue light reduced the deg

299 Cross-linking with riboflavin/UV-A-light reduced the deg

300 e surface, followed by physical and covalent cross-linking with the tissue surface.