ライフサイエンスコーパス: cryopreservation

1 by feeding larvae a proline-augmented diet (cryopreservation).

2 with another method (e.g., embryo or oocyte cryopreservation).

3 an predict sperm quality and fertility after cryopreservation.

4 lied to assess cell envelope integrity after cryopreservation.

5 t to fields as diverse as cloud formation to cryopreservation.

6 to test this method for human ovarian tissue cryopreservation.

7 mem) and ATP-production capacities following cryopreservation.

8 s added to the viability of sperm and oocyte cryopreservation.

9 f fertility preservation by embryo or oocyte cryopreservation.

10 ozole and gonadotropins for embryo or oocyte cryopreservation.

11 ensitive cancers undergoing embryo or oocyte cryopreservation.

12 lls retain their phenotypic properties after cryopreservation.

13 after both transformation and recovery from cryopreservation.

14 ained their phenotype and APC function after cryopreservation.

15 transplantation and five who chose stem-cell cryopreservation.

16 apoptotic death of porcine hepatocytes after cryopreservation.

17 ase in the fraction of motile NK cells after cryopreservation.

18 prove the survival of mammalian cells during cryopreservation.

19 er serum-free conditions for 36 hours before cryopreservation.

20 fetal bovine serum-supplemented media and to cryopreservation.

21 g studied with the potential to enable organ cryopreservation.

22 A total of 591 patients underwent cryopreservation.

23 tial screening tool to identify new CPAs for cryopreservation.

24 icrobial communities using lyophilization or cryopreservation.

25 to the untreated control, cultured prior to cryopreservation.

26 discover, and apply, new chemical tools for cryopreservation.

27 and structurally seven days after thaw from cryopreservation.

28 t potentially detrimental procedures such as cryopreservation.

29 hat it preserved the membrane integrity upon cryopreservation.

30 and potential for differentiation even after cryopreservation.

31 est MSC viability and potency are damaged by cryopreservation.

32 d as a promising alternative to conventional cryopreservation.

33 years with a median 5.25 years after embryo cryopreservation.

34 for selection of patients for ovarian tissue cryopreservation.

35 ) expressions were unchanged after 1month of cryopreservation.

36 ng 4 criteria: (1) offered oocyte and embryo cryopreservation, (2) performed at least 1 FP cycle in 2

37 Embryo cryopreservation after ovarian stimulation with the letr

38 ) that are stable against aggregation in the cryopreservation agent VS55 before and after vitrificati

39 cryopreserved organs perfused with magnetic cryopreservation agents (mCPAs) could increase donor org

40 udies reporting sperm parameters both before cryopreservation and after thawing were included for ana

41 rd commercial heart valve freezing method of cryopreservation and an ice-free, vitrification method o

42 his study demonstrated the successful use of cryopreservation and assisted reproduction to maintain a

43 Despite major advances in tissue cryopreservation and auto-transplantation, reperfusion i

44 Ovarian tissue cryopreservation and autotransplantation can restore ova

45 t, results of the current model suggest that cryopreservation and autotransplantation were cost-effec

46 Details are included for propagation, cryopreservation and characterization.

47 forward programming, combined with efficient cryopreservation and good manufacturing practice (GMP)-c

48 t and provides new insights to improve sperm cryopreservation and in vitro fertilization in the domes

49 haemia followed by reperfusion, we show that cryopreservation and intra-myocardial delivery of one bi

50 ferative, less immunogenic, and resistant to cryopreservation and ischemic injury.

51 valuated ROS in zebrafish spermatozoa during cryopreservation and its effect on viability and motilit

52 Cryopreservation and MMF potentially prevent engraftment

53 motility relative to fresh samples following cryopreservation and post-thaw incubation, as well as ac

54 strong magnetic responsiveness, capacity for cryopreservation and rapid expansion, and the ability to

55 imised and straightforward procedure for the cryopreservation and recovery of both parental and trans

56 Here, we report the successful cryopreservation and revival of cm-scale coral fragments

57 enges with quality, inter-batch consistency, cryopreservation and scale remain, reducing experimental

58 irst amplified PGC numbers in culture before cryopreservation and subsequent transplantation into hos

59 e interstitial ice damage that occurs during cryopreservation and subsequently leads to accelerated v

60 ced PSC (iPSC) derivation, colony expansion, cryopreservation and teratoma formation.

61 r mouse strain maintenance, distribution and cryopreservation and training fellowships in mouse patho

62 Although germ cell cryopreservation and transplantation can help preserve t

63 Cryopreservation and transplantation of gonadal tissue i

64 We aimed to restore fertility by cryopreservation and transplantation of ovarian tissue.

65 e identified from publications in bull semen cryopreservation, and an initial 200 extender-post-thaw

66 egenerative medicine, in vitro drug testing, cryopreservation, and cell isolation/purification.

67 d from resected tumors and biopsies, survive cryopreservation, and exhibit ductal- and disease-stage-

68 em cells has allowed their in vitro culture, cryopreservation, and genetic modification.

69 Determining optimal methods of tissue cryopreservation, and germ cell recovery and transplanta

70 pplications of IBPs in the food industry, in cryopreservation, and in other technologies are vast, an

71 h BBB models: cell source, barrier function, cryopreservation, and matrix stiffness.

72 irst 20 passages of hESC derivation prior to cryopreservation, and that supplementation with either s

73 uction without IL-10 production; (c) ease in cryopreservation; and (d) markedly reduced cost.

74 optosis occurs in porcine hepatocytes during cryopreservation; and 2) mitochondrial injury in this pr

75 ion, large volume apheresis, processing, and cryopreservation appears to be safe in donors with SCT,

76 ional performance was also influenced by the cryopreservation approach employed.

77 Here, we describe a simple and effective cryopreservation approach for sBC cultures that yields h

78 nd embryo cryopreservation as well as oocyte cryopreservation are considered standard practice and ar

79 Sperm and embryo cryopreservation are considered standard practice and ar

80 perimental data on the state of water during cryopreservation are lacking.

81 However, overall, the long-term costs of cryopreservation are low, while the benefits are huge.

82 Success rates with oocyte cryopreservation are on the rise and begin to approach t

83 mic and logistical commitments necessary for cryopreservation are worthwhile for their individual nee

84 rvation techniques, such as semen and embryo cryopreservation, are established and successful in adul

85 as incubation in a physiological medium and cryopreservation, are suboptimal, resulting in significa

86 Sperm and embryo cryopreservation as well as oocyte cryopreservation are

87 llization inhibition activity (IRI) and RBCs cryopreservation assay confirm the pronounced better IRI

88 hods of preservation, we tested hypothermia, cryopreservation at -80 degrees Celsius in DMSO (dimethy

89 unophenotyping panels involving fixation and cryopreservation at three distinct points during the wor

90 urvival in women undergoing embryo or oocyte cryopreservation before adjuvant chemotherapy.

91 In young women, ovarian cortex cryopreservation before gonadotoxic chemotherapy and its

92 ion criteria and were offered ovarian tissue cryopreservation before starting cancer treatment.

93 Cryopreservation by vitrification could transform fields

94 Cryopreservation by vitrification has far-reaching impli

95 urther progress is needed before whole-ovary cryopreservation can be considered an option for safegua

96 Conventional frozen cryopreservation (CFC) is currently the gold standard fo

97 quirements are reliable methods for culture, cryopreservation, characterization and quality control.

98 Cryopreservation combined with electron tomography were

99 model showed no adverse effects of vitreous cryopreservation compared with fresh tissue grafts.

100 Cryopreservation could solve islet supply chain challeng

101 Assess the effects of cryopreservation (cryo) and vitrification (vitro) on the

102 nd allowing other deleterious effects of the cryopreservation cycle to be independently studied.

103 breast surgery enables earlier initiation of cryopreservation cycles and chemotherapy and, when appro

104 rformed to determine the economic utility of cryopreservation/DACP vs usual care (monitoring and supp

105 gen and processed cells were infused without cryopreservation daily for 4 consecutive days.

106 n procuring huMG and their susceptibility to cryopreservation damage have limited large-scale studies

107 Although cryopreservation decreased hepatocyte viability, individ

108 als using cryopreserved sperm (p < 0.05); 3) cryopreservation did not affect settlement success, as l

109 lso been shown to protect sperm cells during cryopreservation due to its micellar structure.

110 due to human impacts, thereby necessitating cryopreservation efforts.

111 tissue structures compared with conventional cryopreservation employing lower CPA concentrations.

112 Cryopreservation enables long-term storage and transport

113 Cryopreservation ensures off-the-freezer availability of

114 wide range of freezing processes, including cryopreservation, food science, and frost heave.

115 ere ice growth control is necessary, such as cryopreservation, food storage, and anti-icing.

116 nsplantation and evaluated methods for ovary cryopreservation for the first time in the white sturgeo

117 ast elimination; and (4) an optional step of cryopreservation for the storage of the excess of cells.

118 tal growth is a major problem in cell/tissue cryopreservation for transplantation, transfusion and ba

119 sent experimental data assessing 5 different cryopreservation formulae for long-term storage of clini

120 Here, 10 different cryopreservation formulations consisting of individual o

121 have a wide range of applications including cryopreservation, frost protection, and as models in bio

122 Cryopreservation has been adopted as a method for long-t

123 over several decades to develop a method for cryopreservation have, until now, been fruitless: we des

124 extra embryos (if embryos are set aside for cryopreservation, higher quality embryos may have been a

125 Ice-free cryopreservation (IFC) already demonstrated matrix struc

126 mamide (FMD), routinely employed in ice-free cryopreservation (IFC), have advantages in long-term pre

127 Cryopreservation in 5% dimethyl sulfoxide/6% pentastarch

128 nburgh selection criteria for ovarian tissue cryopreservation in girls and young women with cancer to

129 s embryo cryopreservation in women and sperm cryopreservation in men before the initiation of cancer-

130 rving fertility in this population is embryo cryopreservation in women and sperm cryopreservation in

131 sh to delay childbearing, which makes oocyte cryopreservation increasingly popular to overcome an age

132 For men, semen cryopreservation is a simple way of preserving reproduct

133 ithout moving adults, we show that AGF using cryopreservation is a viable conservation tool to increa

134 Cryopreservation is a well-established technique used fo

135 Sperm cryopreservation is an effective but underused method to

136 Testicular tissue cryopreservation is an experimental method to preserve t

137 Cryopreservation is an important conservation tool, whic

138 Cryopreservation is an important intervention measure an

139 However, cryopreservation is associated with a loss of hepatocyte

140 Because long-term cryopreservation is critical for CB banking and transpla

141 The mechanism of cell death during cryopreservation is incompletely understood, but may inv

142 For prepubertal boys, testicular biopsy cryopreservation is offered in some centres, but how tha

143 Although prechemotherapy sperm cryopreservation is recommended, treatment with microdis

144 vation of ovarian tissue is an option if egg cryopreservation is ruled out.

145 An established view in cellular cryopreservation is that following freezing, cells must

146 The current state-of-the-art method of cryopreservation is the addition of large amounts of org

147 Ovarian-tissue cryopreservation is used to preserve fertility in childr

148 Among the three defined cryopreservation media (PBS, 7H9, and 7H9 plus glycerol)

149 liquid nitrogen (LN2), because commonly used cryopreservation media containing cell membrane permeati

150 lts of the present study suggested that Lake cryopreservation medium with 0.5-ml straw may perhaps be

151 The ice-free cryopreservation method demonstrated significant reducti

152 better fertility preservation, but the best cryopreservation method is unknown.

153 e donors make it mandatory to use an optimal cryopreservation method that allows maximal recovery and

154 the practicability of a promising long-term cryopreservation method that completely eliminates the n

155 cification is influenced by two factors, the cryopreservation method used and immunogenicity.

156 A glycerol-based cryopreservation method was also tested.

157 A cryopreservation method was established to optimize sour

158 ct the best cryoprotectant solution for each cryopreservation method.

159 To be an effective restoration tool, the cryopreservation methods and husbandry to produce new of

160 Alternative cryopreservation methods that avoid ice formation may im

161 Current red-blood-cell cryopreservation methods utilize bulk volumes, causing c

162 Using modifications of established cryopreservation methods we developed MSC that retain >9

163 technical limitations, including the lack of cryopreservation methods.

164 Both egg and ovarian tissue cryopreservation might be ready for application to the p

165 This implies that suboptimal cryopreservation might not cause severe damage to the ce

166 Cryopreservation modestly affected stem cell memory, act

167 CBU potency is typically assessed before cryopreservation, neglecting changes in potency occurrin

168 ur results present a general solution to the cryopreservation of a broad spectrum of cellular, organi

169 hipathic helix and that it enhances the DMSO cryopreservation of adherent cell lines.

170 fruitless: we describe here a method for the cryopreservation of Anopheles stephensi embryos yielding

171 oprotective agent (CPA) is necessary for the cryopreservation of articular cartilage.

172 s (PGCs) represent an important tool for the cryopreservation of avian genetic resources.

173 Cryopreservation of biological material is vital for exi

174 The vitrification solutions used in the cryopreservation of biological samples aim to minimize t

175 represents a strong practical limitation to cryopreservation of biological systems.

176 a second-generation method is described for cryopreservation of C. parvum oocysts by vitrification u

177 Current long term cryopreservation of cell stocks routinely requires the u

178 Cryopreservation of cells and biologics underpins all bi

179 (IRI) are of critical importance in biology, cryopreservation of cells and organs, and frozen foods.

180 Cryopreservation of cells in sub-milliliter volumes is d

181 eversible injury during freezing/thawing and cryopreservation of cells, but the underlying causes rem

182 The cryopreservation of cells, tissue and organs is fundamen

183 They have potential for the cryopreservation of cells, tissues, and organs, as well

184 bservation of cellular structures as well as cryopreservation of cells.

185 represents the first successful instance of cryopreservation of coral larvae that proceeded to settl

186 is and Acropora millepora and found that: 1) cryopreservation of coral sperm reduced sperm motility a

187 Cryopreservation of cortex ovarian tissue before antican

188 oxicity is the most limiting factor impeding cryopreservation of critically needed tissues and organs

189 While cryopreservation of Cryptosporidium oocysts by vitrifica

190 e technological importance especially in the cryopreservation of donor cells and tissue, but native a

191 The application of these polymers to the cryopreservation of donor cells is also introduced.

192 Cryopreservation of eggs or ovarian tissue to preserve f

193 This study compared protocols for cryopreservation of ejaculated, papain-treated alpaca sp

194 Options for fertility preservation include cryopreservation of embryos or oocytes before chemothera

195 native-immediate in vitro fertilisation with cryopreservation of embryos-is not always appropriate.

196 One marker for embryo quality is cryopreservation of extra embryos (if embryos are set as

197 rnal age, number of embryos transferred, and cryopreservation of extra, nontransferred embryos (an in

198 From derivation to cryopreservation of fully characterized initial stocks t

199 Our approach to conservation is through cryopreservation of germinal cells and tissue.

200 The application of HrCHI4 in cryopreservation of green beans was analyzed to verify i

201 The successful cryopreservation of hBTSCs facilitates establishment of

202 Cryopreservation of hematopoietic stem cells (HSCs) and

203 After collection and cryopreservation of hematopoietic stem cells, 15 patient

204 ng demand for successful, efficient means of cryopreservation of hepatocytes.

205 Vitrification is now the main route to the cryopreservation of human and animal oocytes and preimpl

206 his methodology, including the technique for cryopreservation of human tissue, will allow multiple (s

207 assisted reproduction (MAR) have led to the cryopreservation of hundreds of thousands of embryos eac

208 Application of PWW to the cryopreservation of immortalized T-cells in 96-well plat

209 rch settings, a robust and reliable means of cryopreservation of immune samples is required.

210 of M. tuberculosis complex strains to study cryopreservation of M. tuberculosis complex cells in div

211 Cryopreservation of mature oocytes is also becoming more

212 Successful cryopreservation of most multicompartmental biological s

213 ld woman underwent a right oophorectomy with cryopreservation of ovarian cortical strips before recei

214 For young women, cryopreservation of ovarian cortical tissue with later r

215 Cryopreservation of ovarian tissue has been selectively

216 Cryopreservation of ovarian tissue is an option if egg c

217 This natural cryopreservation of plant tissue over many thousands of

218 research, including routine cell passaging, cryopreservation of pluripotent and differentiated cells

219 -DL-Asp-fluoromethylketone (ZVAD-fmk) during cryopreservation of porcine hepatocytes.

220 wed include processing of testicular tissue, cryopreservation of retrieved spermatozoa, in-vitro matu

221 to antifreeze (glyco)proteins to enhance the cryopreservation of sheep and human red blood cells.

222 We describe successful isolation and cryopreservation of spermatogonia in an Australian rainb

223 actical value, for example, for the possible cryopreservation of stem cells from boys undergoing trea

224 e, it provides an operating envelope for the cryopreservation of T cells as an emerging industry defi

225 Cryopreservation of testicular tissue allows storage of

226 Our results show that cryopreservation of testicular tissue from prepubescent

227 In macrolecithal species, cryopreservation of the oocyte and zygote is not possibl

228 tients are offered fertility preservation by cryopreservation of the ovarian cortex.

229 s required to design optimized protocols for cryopreservation of tissue.

230 d soft biomaterials and is of great value to cryopreservation of tissues, hyperthermia or cryogenic,

231 etal bovine serum (FBS) can provide reliable cryopreservation of various kinds of human and porcine p

232 ication and slow freezing techniques for the cryopreservation of zebrafish ovarian tissue containing

233 be more effective than slow freezing for the cryopreservation of zebrafish ovarian tissue.

234 examined whether antioxidants might improve cryopreservation of zebrafish sperm.

235 , or whose genetic makeup must be preserved, cryopreservation offers the only possibility for long-te

236 Cryopreservation offers the potential to increase the av

237 Herein we examine the effect of cryopreservation on human MSC viability, immunomodulator

238 The influence of cryopreservation on PBPC was tested in vitro by enumerat

239 32 studies demonstrated negative effects of cryopreservation on sperm parameters, Specifically, moti

240 plored the effects of lethal irradiation and cryopreservation on TALL-104 cell viability and lytic ef

241 141 of the 376 patients who were not offered cryopreservation; one of these patients had developed pr

242 pared to slow freezing and may be a valuable cryopreservation option.

243 As its use preceded development of either cryopreservation or cell culture, the vaccine strain was

244 including embryo, oocyte, and ovarian tissue cryopreservation (OTC), ovarian transposition, and conse

245 regarded as experimental (eg, ovarian tissue cryopreservation, pharmacological protection against gon

246 inally, we detail a complete prototype coral cryopreservation pipeline, which provides a platform for

247 eservation (FP), including oocyte and embryo cryopreservation prior to gonadotoxic therapy, is an urg

248 Next, cryopreservation prior to manufacturing is discussed in

249 We show that the cryopreservation procedure is equally applicable to the

250 Further, the cryopreservation procedure kept sensitivity unaltered fo

251 The development of cryopreservation procedures for tissues has proven to be

252 Here, we present effective cryopreservation procedures of the human hair follicle t

253 it can be readily incorporated into existing cryopreservation procedures.

254 nerating viable pregnancies or surviving the cryopreservation process are also a viable source of hES

255 when viability is maintained throughout the cryopreservation process, MSC retain their therapeutic p

256 e modified to improve the reliability of the cryopreservation process, perhaps using a different cryo

257 rall, the results suggest that our optimized cryopreservation protocol holds promise to preserve the

258 re we report a robust and easily implemented cryopreservation protocol of Drosophila melanogaster emb

259 Last, we use this cryopreservation protocol with radioactive specimens to

260 transplantation and to inform the design of cryopreservation protocols for other multicellular aggre

261 Cryopreservation protocols have been developed that mini

262 Thus, the addition of trehalose to cryopreservation protocols leads to previously unobtaina

263 However, developing new cryopreservation protocols requires exploration of a com

264 There are improved cryopreservation protocols, but these need further refin

265 Cryopreservation provides a critical tool for dairy herd

266 assessment for treating various cancers, but cryopreservation reduces both the recovery and function

267 Ice-free cryopreservation, referred to as vitrification, is recei

268 nt, lipid-producing alga, under a variety of cryopreservation regimes.

269 sment of cellular response to IL-6 following cryopreservation revealed a similar pattern of up-regula

270 In contrast, damage caused during cryopreservation significantly affects the overall tensi

271 ients who had successfully undergone ovarian cryopreservation, six had developed premature ovarian in

272 e cryoprotectants and enable next-generation cryopreservation solutions for the cold chain.

273 Cryopreservation strategies aim to increase the viable s

274 lay the groundwork for developing SRS-guided cryopreservation strategies in D. magna, with potential

275 ls science tools, which are set to transform cryopreservation strategies, are also included.

276 cts of long-term freezing not found in other cryopreservation studies that lacked functional regenera

277 invasive imaging and particular relevance to cryopreservation studies.

278 Customizing cryopreservation techniques and cryoprotective agents (C

279 pecific landscape of available and effective cryopreservation techniques, preventing trial-and-error

280 ces in cryogenic biological applications and cryopreservation techniques.

281 onments, and plays a central role in various cryopreservation techniques; its formation is observed o

282 ents who met the criteria for ovarian tissue cryopreservation than for those who did not (15-year pro

283 hermore, we have demonstrated the successful cryopreservation, thawing, and induction of pathogenic A

284 Together with the possibility of cryopreservation this shall foster diffusion and exploit

285 he resulting CAR T cells were formulated for cryopreservation to be used directly for infusion into p

286 Cryopreservation, using either DMSO or glycerol as a pro

287 s (ICCs) were more pronounced: recovery from cryopreservation was 94%, compared with 42% without treh

288 stimulation (OS) for FP by oocyte or embryo cryopreservation was performed.

289 The efficacy of cryopreservation was quantified by microbiological cultu

290 t of reducing the variability in mouse sperm cryopreservation, we examined whether antioxidants might

291 ls with their respective water states during cryopreservation, we found that survival is less depende

292 ion from the wild in 1951, before methods of cryopreservation were developed.

293 that MSCs may lose their functionality with cryopreservation which could render them non-efficacious

294 Recovery of adult islets after cryopreservation with 300 mmol/l trehalose was 92 vs. 58

295 ion and an ice-free, vitrification method of cryopreservation with fresh controls in a subcutaneous,

296 Ovarian tissue cryopreservation with later reimplantation has been show

297 demonstrated and will allow routine ice-free cryopreservation with smaller CPA concentrations.

298 sfully manufactured and administered without cryopreservation within 72 hours.

299 ic polymer-based approach for red-blood-cell cryopreservation without the need for any (toxic) organi

300 ing and the number of samples needed to make cryopreservation worthwhile.