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1 rder is required for them to be an effective cryoprotectant.
2 servation process, perhaps using a different cryoprotectant.
3 tical solvent conditions, using glucose as a cryoprotectant.
4                  Current methods use DMSO as cryoprotectant.
5 y rapid warming with concomitant dilution of cryoprotectant.
6  extenders, then subsequently mixed 1:1 with cryoprotectant.
7 , with DMFA being the likely preferred sperm cryoprotectant.
8 tive in the emerging field of macromolecular cryoprotectants.
9 phases and the impact of membrane-protective cryoprotectants.
10 ress by adding and removing high contents of cryoprotectants.
11 ent accumulation of betaine and carnitine as cryoprotectants.
12  the rapid exchange of media ingredients and cryoprotectants.
13 , buffers, and in the presence or absence of cryoprotectants.
14  novel guidelines for the rational design of cryoprotectants.
15 ty to follicles caused by elevated levels of cryoprotectants.
16 to the development of the next generation of cryoprotectants.
17  at - 80 degrees C without glycerol or other cryoprotectants.
18 n exposure, even in the absence of canonical cryoprotectants.
19 he active site immobilizes a molecule of the cryoprotectant 2-methyl-2,4-pentanediol.
20 cy of UAF to comparable levels of commercial cryoprotectant (4% sucrose with 4% sorbitol).
21                                              Cryoprotectants, 8% methanol and 10% dimethylacetamide (
22 ce methodology revealed little difference in cryoprotectant ability between FPHs produced from Pacifi
23 eta) islets by comprehensive optimization of cryoprotectant agent (CPA) composition, CPA loading and
24 dy addresses these challenges by integrating cryoprotectant agent (CPA) optimization, advanced therma
25 ment of the intercellular concentration of a cryoprotectant agent (dimethylsulfoxide), and the distri
26 ent innovations for embryo permeabilization, cryoprotectant agent loading, and rewarming.
27 the largest and most comprehensive screen of cryoprotectant agents (CPAs) was performed to determine
28 nd freeze-avoiding insects, functioning as a cryoprotectant and a supercooling agent.
29 ells formulated in a conventional DMSO based cryoprotectant and processed in conventional cryovials.
30 Fourth, we examined the effect of additional cryoprotectants and CAT on fresh sperm motility.
31 pproaches can be used to discover innovative cryoprotectants and enable next-generation cryopreservat
32 demonstrated significantly higher amounts of cryoprotectants and enhanced activities of enzymatic and
33  cryopreservation efficiency, the effects of cryoprotectants and molecular processes need to be under
34 d structures to facilitate rapid delivery of cryoprotectants and protect complex neuronal structures
35 en broadly used in biology as a cosolvent, a cryoprotectant, and an enhancer of membrane permeability
36                                              Cryoprotectants are loaded ex vivo using subnormothermic
37 on media containing cell membrane permeating cryoprotectants are thermally unstable when frozen at hi
38 b interactions renders these known osmolytes cryoprotectants as well as osmoprotectants, explaining w
39 , S1-binding alcohol, and ethylene glycol (a cryoprotectant), as well as a ternary trypsin, borate, a
40                   By fixing tissues, using a cryoprotectant before freezing, and using an adhesive-co
41 e dimensional reduction strategy to improved cryoprotectant behaviors.
42                           This suggests that cryoprotectants can act by inhibiting crystallization or
43 acturing is discussed in relation to timing, cryoprotectants, concentration, cooling rates, and cellu
44 , while decreasing the toxic effects of high cryoprotectant concentrations.
45 ding guiding tools to the rational design of cryoprotectant containing nano formulations and processe
46 pon nanoparticles drying, the utilization of cryoprotectants could cause less aggregation and better
47 ne extraction have been studied with/without cryoprotectants (CP) after 3 weeks of frozen storage.
48 is the requirement of high concentrations of cryoprotectant (CPA) chemicals and the damage caused by
49 he plasma membrane permeability to water and cryoprotectant (CPA) significantly impacts vitrification
50 Toxicity experiments were conducted with the cryoprotectants (CPAs) ethylene glycol (EG), propylene g
51 d be a viable alternative to the sugar-based cryoprotectants currently used for frozen fish products.
52  obtain good agreement between the water and cryoprotectant densities obtained from the simulated cry
53  (PBPCs) are commonly cryopreserved with the cryoprotectant dimethyl sulfoxide (DMSO), which can caus
54  images of the spatial distribution of three cryoprotectants (dimethyl sulfoxide, propylene glycol, a
55                 This study aimed to test two cryoprotectants, dimethyl formamide (DMFA) and dimethyl
56 droplet radius during the vitrification of a cryoprotectant droplet in the presence of the Leidenfros
57            A molecule of glycerol, used as a cryoprotectant during diffraction experiments, is seen t
58 egmatis) revealed that by removing salts and cryoprotectant (e.g., glycerol) from bacterial suspensio
59 ss is still limited compared to conventional cryoprotectants, e.g., sugars, polyols, especially at hi
60  We postulate that DMSO acts as an efficient cryoprotectant even at low concentrations by exclusively
61                       In contrast, the other cryoprotectants exhibited little or no permeation over 2
62 bull post thaw fertility, recent research on cryoprotectant extender medium has not dramatically impr
63                          The most successful cryoprotectant for "large cell" (> 9 mum) viability (72.
64 ation of fish protein hydrolysates (FPHs) as cryoprotectants for cod fish mince subjected to freeze-t
65 identified, which we attribute to the use of cryoprotectants for PELDOR/DEER and label-protein intera
66          Transport of the osmoprotectant and cryoprotectant glycine betaine was investigated in membr
67 on photoperiod accumulated large reserves of cryoprotectants (i.e. 'antifreeze') and exhibited greate
68       MR spectroscopy and microinjections of cryoprotectants into the yolk inferred that the yolk syn
69                           A 7.5 M mixture of cryoprotectants known as VS4 is sufficiently concentrate
70 f flavourzyme hydrolysate as the alternative cryoprotectant might be employed during crustacean proce
71 nded streptavidin crystal lattice, including cryoprotectant molecules and crystallization salts, is c
72   Here, we discover the regulatory effect of cryoprotectants on ice crystal growth and use this prope
73 in the concentration of various metabolites (cryoprotectants) or in the composition of amino acids an
74 option of glycerol and dimethyl sulfoxide as cryoprotectants over 60 years ago, but these tools do no
75 - 10.5%) and significantly better than other cryoprotectants (p < 0.0006).
76                                     Although cryoprotectant perfusion was considered normal and no ic
77 This report describes direct measurements of cryoprotectant permeation into a multicompartmental syst
78 e for success is quantitative information on cryoprotectant permeation into and amongst the compartme
79 to variation in photoperiod and measured its cryoprotectant reserves (glycogen stored in the liver),
80 on and oxidation similarly to the commercial cryoprotectants, resulting in higher protein solubility
81             In both cases, glycerol from the cryoprotectant solution became liganded to the vanadate
82 clusion staining was used to select the best cryoprotectant solution for each cryopreservation method
83 d by an acetate ion derived from the crystal cryoprotectant solution.
84 we demonstrate that addition of Ficoll 70 to cryoprotectant solutions significantly improves system t
85 ated by addition of glucose, which acts as a cryoprotectant through preferential exclusion from side
86 ical role in limiting the permeation of some cryoprotectants throughout the embryo.
87 efine the boundary condition of the minimal 'cryoprotectant to particle ratio' required for effective
88 that combines ex vivo machine perfusion with cryoprotectants to facilitate long-term supercooled pres
89 otocol describes how to load rat livers with cryoprotectants to prevent both intracellular and extrac
90 -tolerant species have AFPs that function as cryoprotectants to prevent freeze damage.
91  of cellular solutes by air drying or adding cryoprotectants, together with rapid cooling, results in
92 rm-to-egg ratio and the concentration of the cryoprotectant treatments affected fertilization success
93 es, low oxidative stress and accumulation of cryoprotectants under cold stress were associated with h
94 ability while minimizing shear stress during cryoprotectant unloading with an acclimation period and
95 ess of freezing in aqueous droplets provided cryoprotectants were utilized.
96 uch, it can be regarded as a unique class of cryoprotectant which acts by inducing ice nucleation at
97 rbitol is a widespread compatible solute and cryoprotectant, which suggests its participation in tole
98 iscovered usage of Au colloids for advancing cryoprotectants with significant ice recrystallization i
99 buted to extremely weak interaction of these cryoprotectants with the T state of the enzyme.