ライフサイエンスコーパス: culture of

1 trated an odds ratio (OR) for positive viral culture of 0.64 (95% confidence interval [CI], .49-.84;

2 eus isolates were collected from respiratory cultures of 115 CF patients during a 22-month study peri

3 Efm isolates from gastrointestinal and blood cultures of 24 pediatric patients undergoing chemotherap

4 Cultures of 3 species were subcultured from 3 to 5 times

5 ells (hPSCs), many of which are based on the culture of 3D organoids.

6 In this study, we have isolated a pure culture of a bacterium, strain MG08 that grows on air at

7 llurium particles [Te(0)], as harvest from a culture of a tellurium-oxyanion respiring bacteria.

8 lar delivery was achieved in all cells in 2D cultures of a human bladder epithelial progenitor cell l

9 es after the addition of ferrihydrite to the cultures of a methanogenic archaeon, Methanosarcina bark

10 tment plants, which can be achieved using co-cultures of a specialized pollutant degrader with combin

11 Incubations with pure and enrichment cultures of Acidimicrobium sp. strain A6 (A6), an autotr

12 Integrating 2D culture of adherent mammalian cells with single-cell wes

13 This approach substantially prolongs the culture of adult cardiac tissue in vitro.

14 We established conditions for long-term culture of adult mouse cardiomyocytes that are genetical

15 current are significantly reduced in primary cultures of Ahnak knockout (KO) neurons compared to wild

16 osity, and antibacterial activity in primary cultures of airway epithelia from people with cystic fib

17 ll reprogramming, we generated a stable cell culture of an extremely rare and aggressive neuroendocri

18 Improving the research culture of an institution may lead to a fairer, more rew

19 tionality in the maintenance of master stock-cultures of an industrially relevant, lipid-producing al

20 tform (LISCCP) that facilitates digital mass culture of anchorage-dependent cells.

21 an provide a unique window into the culinary cultures of ancient people, resource use, and environmen

22 We also show that the culture of animals in pillar-less microfluidic chambers

23 periprosthetic tissue culture and sonication culture of antimicrobial agent-containing cement spacers

24 this study, we established organotypic cell cultures of AT explants to study the impact of cytokine

25 radation of trifluralin does occur, and pure cultures of bacteria and fungi capable of partially degr

26 An enrichment culture of basaltic sediments provided with H(2), CO(2),

27 Laboratory culture of bioluminescent organisms from diverse taxonom

28 their biosafety practices and improve their culture of biosafety.

29 ng for histopathology examination; microbial culture of blood, cerebrospinal fluid (CSF), liver, and

30 In vitro culture of BMDN with rmCIRP significantly increased NETo

31 Co-culture of bone marrow-derived macrophages with organoty

32 n osteoclastogenesis was greatly enhanced in cultures of bone marrow-derived macrophages (BMMs) from

33 Working with a large continuous laboratory culture of both the slugs (>500 individuals) and their p

34 Here, we show that in cultures of brain ECs, EphB4 stimulates the VE-cadherin/

35 Quantitative bacterial culture of bronchoalveolar lavage fluids (BALF) is labor

36 ated anaerobic digester sludge (AS) and mono-culture of C. kluyveri (Ck).

37 ed microscopy-based assays on 3D organotypic cultures of Caco-2 cysts as a model system.

38 ied lack of qualified candidates and overall culture of cardiology as the 2 most significant barriers

39 However, tissue cultures of cardiomyocyte monolayers currently require t

40 left ventricular cardiac tissue and primary cultures of cardiomyocytes from fetal (100- and 135-d ge

41 cal property decrease during 24-day in vitro culture of cartilage explants.

42 p to 1.2-fold greater in furfural-challenged cultures of Cb_3974 relative to Cb_3904 and Cb_wild type

43 In vitro analyses using primary cultures of CD47-deficient murine colonic IEC or human c

44 Sonication culture of cement spacers identifies a similar proportio

45 on assay confirmed this close interaction in cultures of cerebellar granule cells.

46 flow aligned microvessels generated from co-cultures of cerebral-derived endothelial cells and peric

47 lation of Pkd1, Stat3, and cilia, as well as cultures of cilia-deficient or STAT3-deficient tubular c

48 Culture of clinical isolates and identification were per

49 dance of cfDNA with standard microbiological culture of contemporaneously collected patient sputum wa

50 ere determined in air-liquid interface (ALI) cultures of control and asthmatic primary human bronchia

51 t reporting, self-correction, replication, a culture of critique, and controls for bias.

52 itutional efforts should be made to foster a culture of cybercivility.

53 Co-cultures of D.bruxellensis/S.cerevisiae were conducted u

54 , using clonal (i.e., genetically identical) cultures of Daphnia pulex, we isolated the contributions

55 s in 22 women (aged 19-81 years) and primary cultures of dermal fibroblast and dermal sheath cells.

56 ed peptide selection was conducted in axenic cultures of Dhc strains 195, FL2, and BAV1.

57 H2 production was observed in dark anoxic cultures of diatoms (Fragilariopsis sp.) and a chlorophy

58 encies (0-300 Hz), which enables multiple co-culture of different cell types with or without electric

59 In vitro cultures of different cell types (monocyte-derived dendr

60 del for pigs as well as air-liquid interface cultures of differentiated airway epithelial cells.

61 d we conducted in vitro studies with primary cultures of differentiated human adipocytes and muscle.R

62 Similar cells were present in primary cultures of dissociated cells from E10.5 embryonic head.

63 ty on three different studies using in vitro cultures of dissociated neurons.

64 Primary co-cultures of DPSC and TGNC were established to evaluate t

65 SIBO was evaluated by bacterial culture of duodenal aspirate, glucose and lactulose brea

66 ontinental distribution and ready laboratory culture of E. muelleri make this a highly practical mode

67 :1 and 5:1 respectively, and two with a pure culture of each strain.

68 England's prior and unique proto-capitalist culture of economic liberty and individualism.

69 Co-culture of EECM-BMEC-like cells with hiPSC-derived smoot

70 esulted in neuronal atrophy in DIV7 cortical cultures of either from E18 transgenic mouse model or fr

71 ogenous SV2A was expressed in mouse neuronal cultures of either sex, which had been depleted of endog

72 Whereas most SB insertions were lost during culture of EL cell lines, Erg insertions were retained,

73 In explant cultures of embryonic kidney rudiments, retinoic acid st

74 Using primary neuronal cultures of embryonic mice of both sexes, we here report

75 inistration (FDA) approved for transport and culture of enteric bacterial pathogens, the FecalSwab ha

76 Here, we demonstrated that culture of EpCAM(+) cells derived from human induced plu

77 ition of common alkylating agents to growing cultures of Escherichia coli leads to the accumulation o

78 tories and is designed to cultivate a campus culture of ethical science and engineering research in t

79 The academic environment must establish a culture of excellence and faculty engagement, leading to

80 Here we report that cultures of expanded potential stem cells can be establi

81 The effect of conditioned media from the culture of fat with ROS or PGZ on i) platelet-derived gr

82 The development of differentiated primary cultures of ferret nasal epithelial cells is an importan

83 A contact co-culture of fluorescently labeled GBM and MG demonstrated

84 SPR/Cas9 system and differentiated into pure cultures of forebrain excitatory neurons without contami

85 Organotypic 3D culture of fresh tumor tissue enables convenient real-ti

86 tion in reconstituted three-dimensional cell culture of fully differentiated human bronchial epitheli

87 Primary cultures of GCPs from Jdp2-KO mice at postnatal day 5 we

88 In primary cultures of glial cells and in the in vivo mouse model,

89 rmed in A549 cells, plus submersion, and ALI culture of HBE cells.

90 No growth was observed in the cultures of heat-treated samples.

91 sulting PDMS devices supported physiological cultures of HeLa cells and ovarian tissues in vitro, wit

92 fection research, including strategies of co-culture of helminths or their products with organoids an

93 microtissue (MT) model (MT composed of mono-culture of hepatocytes or two different co-culture MT sy

94 Primary cultures of hepatocytes derived from wild-type or hepato

95 Gram stain as well as multiple cultures of her ascites fluid were both negative.

96 In primary cultures of hippocampal neurons from knockouts, NMDA had

97 al network activity in developing and mature cultures of hippocampal neurons.

98 tic vesicles (OVs) when compared to isogenic cultures of hiPSC-derived forebrain neurospheres.

99 We apply this method to a laboratory culture of Histophilus somni, prepared from a single col

100 However, cultures of hNSPCs are heterogeneous, containing cells l

101 Co-culture of hPSC-CFs with hPSC-derived cardiomyocytes dis

102 We revealed that culture of hPSC-HPs in HSC expansion conditions (SFEM wi

103 We discovered that culture of HSCs in low calcium increased their maintenan

104 In our conditions, neither classical co-cultures of HSPCs with primary ECs or MSCs, even in comb

105 This long-term, feeder-free culture of human distal lung organoids, coupled with sin

106 well as novel 2D and 3D human cell-based co-culture of human hepatocytes, KCs (Kupffer cells), LECs

107 Here, using 3D culture of human HSPCs in a degradable zwitterionic hydr

108 eral studies have examined the concept of co-culture of human induced pluripotent cells (hiPSCs) with

109 To promote long-term culture of human islets in PIM-R medium (used for islet

110 Prolonged in vitro culture of human macrophages was used to evaluate the ca

111 syndrome patient samples, in vitro after the culture of human peripheral blood neutrophils with recom

112 Co-culture of human periventricular endothelial cells with

113 tures, invaginating from a three-dimensional culture of human pluripotent stem cells.

114 We utilized a primary culture of human podocytes and 2 mouse models, the wild

115 ed the effect of recombinant uPAR on primary culture of human podocytes.

116 he effects of conditioned media from primary cultures of human ADPKD cystic epithelial cells on myofi

117 signature obtained from air-liquid interface cultures of human bronchial epithelial cells stimulated

118 transport, we studied differentiated primary cultures of human cystic fibrosis (CF) and non-CF airway

119 Here we employ explant cultures of human fetal organs (adrenals, gonads, genita

120 on profiles (RNA-seq) from primary outgrowth cultures of human glomeruli that were composed mainly of

121 Spheroid cultures of human HepaRG progenitors (HepaRG-Spheres), H

122 Co-cultures of human hepatoma and hepatic stellate (HSCs) c

123 actor cocktail containing FGF2 and IGF1 from cultures of human inter-follicular keratinocytes (KC) is

124 ctively reduced the proliferation of primary cultures of human pleural (Pl) MM, implicating nonepithe

125 we suppressed miR-204 expression in primary cultures of human RPE using anti-miR-204.

126 Using cultures of human trophoblasts or mouse cells, we show t

127 The microfluidic culture of IEC lead to an increased polarization and dif

128 he findings of this study do not support the culture of induced sputum specimens as a diagnostic tool

129 Co-cultures of induced pluripotent stem cell-derived motor

130 rodrugs were potent inhibitors of HIV-1/2 in cultures of infected CEM/0 cells and more importantly in

131 drugs against HIV-1 and HIV-2 replication in cultures of infected wild-type CD4(+) CEM T-cells and mo

132 thway, became perturbed only after long-term culturing of infected cells.

133 Cultures of inflammatory-type material obtained by US-gu

134 Culture of internal organs and 16S rDNA sequencing revea

135 This platform could support long-term culture of intestinal organoids, potentially replacing t

136 Direct co-culture of iPS-HSCs with iPS-HPCs significantly improved

137 t, and cellular homeostasis during long-term culture of iPSC-CMs.

138 l progression, as recapitulated by long-term culture of iPSC-CMs.

139 In addition, culture of islets on 3D-ECM promoted recovery of vascula

140 Cultures of isogenic induced pluripotent stem cell (iPSC

141 Co-culture of isolated naive-like B cells from NSCLC patien

142 xic population of newts and established pure cultures of isolated bacterial symbionts from toxic newt

143 chemia-reperfusion injury model, and primary cultures of isolated tubular cells in a hypoxia-reoxygen

144 soaked for 2-3 h prior to sowing in 24 h old cultures of isolates.

145 )-mediated signaling was assessed in primary cultures of Kupffer cells from ethanol- and pair-fed rat

146 The binary culture of L. plantarum AFI5 and S. cerevisiae AYI7 had

147 Co-culturing of Lactobacillus plantarum with Acetobacter sp

148 Co-culture of Lb. plantarum with acid-producing Lc. lactis

149 ection rate was significantly enhanced by co-culture of leukocytes with cell lines prior to molecular

150 The culture of live pancreatic tissue slices is a powerful t

151 could show that addition of BMP9 to primary cultures of LSECs prevented the loss of their fenestrae

152 In contrast, co-culture of LVS-infected macrophages with LVS-immune lymp

153 The addition of B. plicatilis to healthy cultures of M. salina resulted in decreased algal cell n

154 Treatment of exponentially growing cultures of M. tuberculosis with INH reproducibly kills

155 Additionally, neural cultures of Machado-Joseph disease patients' induced plu

156 In contrast, co-culture of macrophages with cystinotic fibroblasts yield

157 this aim, neutrophils were incorporated into cultures of macrophages treated with IFN-gamma or IL-17A

158 Culture of MAIT cell supernatants with B cells led to gr

159 Eagle's medium (DMEM), other media used for culture of mammalian and yeast cells and phosphate-buffe

160 ased scaffolds support the three-dimensional culture of mammalian cells in tissue-like environments.

161 Overnight culture of MC with compound 48/80 resulted in reduced ce

162 Using three-dimensional cultures of MDCK cells to model cystogenesis in vitro, w

163 Co-culture of MDSCs or macrophages with ddh/ldh1/ldh2 mutan

164 ere a versatile system using cell suspension culture of Medicago sativa, which ensures control over t

165 ainly consist of stripped bark or suspension cultures of members of the plant genus Taxus.

166 Bacterial culture of mesenteric lymph nodes in these mice isolated

167 wo cell types, we tested them in dissociated cultures of MG using either mimics or antagomiRs to incr

168 ake of D-serine in synaptosomes and neuronal cultures of mice of either sex, while increasing the ext

169 Dynamic culturing of microcarriers showcased their great potenti

170 es emitted from healthy and rotifer infested cultures of Microchloropsis salina.

171 reproducible protocol for the isolation and culture of MLECs from adult mice using collagenase I-bas

172 e of worse than 20/40 Snellen and a positive culture of more virulent bacteria (gram-negative and oth

173 In vitro obese models were established by co-culture of mouse adipocytes 3T3-L1 with androgen-sensiti

174 This approach allowed the long-term culture of mouse myeloid progenitors (HoxB8 progenitors)

175 their scrambled counterparts, when added to cultures of mouse cerebellar neurons and human neuroblas

176 n cell identity, we established synchronized cultures of mouse embryonic stem cells as they exit the

177 ion of primordial follicles were assessed in cultures of mouse fetal ovaries from the onset of meioti

178 IV-induced inflammation in vitro, in primary cultures of mouse glial cells and in vivo, in a mouse mo

179 s (rosiglitazone or pioglitazone) in primary cultures of mouse glial cells reversed EcoHIV-induced in

180 ed the motility of Rab5 endosomes in primary cultures of mouse hippocampal pyramidal cells by live-ce

181 ference screen of mammalian chemokines in co-cultures of mouse PDAC cells (K8484) and mouse periphera

182 of a gp41 CT truncation mutant to spread in cultures of MT-4 cells.

183 Culture of multiple periprosthetic tissue samples is the

184 of IL-13-induced lung pathology and in vitro culture of murine fibroblast cell lines and primary fibr

185 Organotypic cultures of murine outer ear and vibrissal skin entrain

186 Myogenesis is recapitulated in the tissue culture of myoblasts that differentiate by fusion and th

187 Addition of recombinant Shh to cultures of naive human CD4+ T cells in iTreg culture co

188 s producers of anti-PS antibodies in ex vivo cultures of naive human peripheral blood mononuclear cel

189 Co-culture of neoplastic cells with CAFs led to increased i

190 Strikingly, in human differentiated co-cultures of neuronal and glial cells, the preferential i

191 However, in vitro culture of neurons deprives them of their natural enviro

192 imilar observations were made in the primary cultures of neurons derived from the hippocampi of the e

193 Co-culture of neutrophils with resting endothelium prevente

194 ents with murine xenografts and 2D and 3D co-cultures of NHFs and PDAC cells revealed that older NHFs

195 effect of IL-15 or TGF-beta on mTOR, as the culture of NK cells in the presence of mTOR inhibitors,

196 to axonal stress are also evident in primary cultures of Nmnat2 compound heterozygote superior cervic

197 o measure effective evolutionary games in co-cultures of non-small cell lung cancer cells that are se

198 The in vitro cultures of normal Tregs in the presence of SP led to a

199 presence of 5BVdU plus IFN-alpha; and (v) 3D cultures of NPCs are less susceptible to HSV-1 infection

200 on addresses a primary culprit, which is the culture of null hypothesis significance testing that dom

201 biosafety approach for in vitro and in vivo culture of O. tsutsugamushi and R. typhi would require t

202 Healthcare is delivered in a culture of ongoing change, with many nurses highlighting

203 ssion and glucose consumption in the primary culture of osteoblast lineage cells, and deletion of Glu

204 CSC-enriched spheroids compared to monolayer cultures of ovarian cancer cell lines or primary cells.

205 esult, establish a system for the continuous culture of P. vivax.

206 d from infected individuals or from in vitro cultures of P. falciparum, making them prone to high var

207 Co-culture of PA5 cells with a neuronal cell line (NG108-15

208 When primary cultures of Pam (0-Cre-cKO/cKO) atrial myocytes (no Cre

209 We optimized the culture of pancreatic tumor 3D clusters that utilized Ma

210 reased beta-cell differentiation in in vitro cultures of pancreatic buds.

211 Culture of PDAC cells with conditioned media from radiot

212 Short-term cultures of PDTX models with increased DeltaG4R levels a

213 ts of intraocular samples included bacterial culturing of pediatric blood culture bottles; 16SrDNA am

214 Herein, we examined the impact of culture of periprosthetic tissues in blood culture bottl

215 , although rifampicin resistance in positive cultures of PICC tips was higher in the antimicrobial-im

216 Culturing of pituitary cells in GnRH-free conditions dow

217 ssion and function were assessed in in vitro cultures of plaque-resident cells.

218 and occurrences of neighbour exchange within cultures of pluripotent cells during differentiation.

219 linary initiative was designed to change the culture of postoperative opioid prescribing, including:

220 er larger bacteria and inoculating them into cultures of potential host bacteria.

221 ropose here, in vitro expansion and extended culture of primary CD4(+) T cells isolated from virally

222 based on a method previously established for culture of primary human bronchial epithelial cells.

223 ganoid culture methods now allow the routine culture of primary human tumor biopsies and increasingly

224 ong axis can be generated, enabling scalable culture of primary in vitro colonic epithelial replicas.

225 o this process we profiled the effects of co-culture of primary MSCs with validated bone metastatic p

226 ng mass spectrometry in fully differentiated cultures of primary human airway epithelial cells and co

227 Using air-liquid interface cultures of primary human bronchial epithelial cells der

228 t expanded the number of T(reg) cells within cultures of primary human CD4(+) T cells from healthy do

229 Air-liquid interface cultures of primary nasal epithelial cells were used to

230 he unique ability to selectively support the culture of primitive human uSPG.

231 ch requires 3 major processes: isolation and culture of primordial germ cells (PGCs), modification of

232 Cultures of Prochlorococcus, the most abundant phytoplan

233 scued by human INPP5B insertion, and primary culture of proximal tubule cells (mPTCs) derived from Oc

234 Analysis of Pi transport in primary cultures of proximal tubular cells or in freshly isolate

235 s were simultaneously visualized from single cultures of Pseudomonas aeruginosa at about 50 mum resol

236 leadership (e.g., leaders who communicate a culture of quality improvement), staffing (e.g., lower n

237 to examine gene expression in continuous co-cultures of R. bromii and B. hydrogenotrophica.

238 es into an extended extracellular network in cultures of rat chondrosarcoma cells.

239 have investigated this phenomenon in primary cultures of rat cortical neurons using overexpression of

240 ive CD and five control subjects, in primary cultures of rat enteric neurons and in nuclear factor er

241 an affect glutamate neurotoxicity in primary cultures of rat hippocampal neurons.

242 ward historical accountability and an active culture of remembrance.

243 The culture of removed cardiac tissues during cardiac surger

244 Bacterial growth in cultures of resected heart valves of patients with infec

245 is work was to evaluate how the use of mixed cultures of Saccharomyces cerevisiae and Lachancea therm

246 The cultures of Saccharomyces cerevisiae were treated with p

247 g between HAS1pr-TDA1pr alleles in saturated cultures of Saccharomyces yeast is mediated by three tra

248 havior, as evidence suggests it undermines a culture of safety.

249 te the development and maintenance of robust cultures of safety at U.S. academic institutions.

250 It is time to change the culture of science by putting inclusive diversity at the

251 ns and scientific initiatives to improve the culture of science in general by equitably incorporating

252 for cultivating a more prosocial and diverse culture of science.

253 with monitoring secretion efficiency in cell culture of selected N2 single-domain mutants, finding th

254 Co-culture of selected products in the presence of vaginal

255 The study aimed to initiate in vitro cultures of selected species of Pleurotus: P. citrinopil

256 Using in vitro cultures of several cell types found in the heart, we de

257 macrophage adhesion to SIRPalpha; and (e) co-culturing of SIRPalpha- and Mac-1-expressing HEK293 cell

258 tion of TGF-beta signaling inhibitor in cell cultures of Smad7(-/-) molar tooth germs, with rescued e

259 hat the addition of ascorbic acid to ex vivo culture of sorted CD56(bright) NK cells increased the fr

260 until September 2017, when direct PCR (with culture of specimens if a carbapenemase gene was detecte

261 In primary cultures of steroidogenic small luteal cells (SLCs), LH,

262 In cultures of striatum or ventral midbrain, CHL1 was also

263 dy viewed coaching as the way to improve the culture of surgery.

264 vice based on inertial sorting for perfusion culture of suspended mammalian cells.

265 In vitro cultures of synthetic VSMCs showed decreased expression

266 T cell therapies require the removal and culture of T cells ex vivo to expand several thousand-fo

267 g frustule biosynthesis in synchronized cell cultures of T. pseudonana.

268 cally focused on two model systems, in vitro culture of the human parasite Plasmodium falciparum and

269 Culture of the lymphoma cells in simulated microgravity

270 We grew a dense culture of the model cyanobacterium Synechocystis sp. PC

271 ort a microfluidic device for the whole-life culture of the nematode Caenorhabditis elegans that allo

272 ontents were not strongly affected by the co-culture of the probiotic and sprouts.

273 the same trend as that observed for the pure culture of the strain that was present in a higher propo

274 e of waste from other processes, promoting a culture of the use of environmentally-friendly technolog

275 This relationship was also observed in cultures of the bloom-forming diatom Chaetoceros tenuiss

276 Microbial swab cultures of the conjunctiva both before instillation of

277 The absence of prophages in cultures of the dominant lineages of marine bacteria has

278 onable and reproducible amounts using axenic cultures of the endophyte.

279 s in self-oscillating autotrophic continuous cultures of the gas-fermenting acetogen Clostridium auto

280 Moreover, cultures of the human melanoma cell line C81-61 with ser

281 s: (1) the diversity both within and between cultures of the many different faces of obligation; (2)

282 repeatedly for the purpose of isolating pure cultures of the microbe responsible for conferring the n

283 PTPD MCSs and two-dimensional (2D) monolayer cultures of the same set of cell lines.

284 n increases with doubling time in continuous cultures of the thermoacidophile Sulfolobus acidocaldari

285 Furthermore, culturing of the cells in vitro, freezing/thawing, reint

286 sion phenotype can be achieved by continuous culturing of the W2mef and Dd2 P. falciparum strains in

287 , tissue secretome was obtained from ex-vivo culture of these paired tissue samples.

288 Robust culturing of these cells on collagen-fibroblast construc

289 our spheroids, including when assessed in co-cultures of TNBC cells and cancer-associated fibroblasts

290 hput screening of chemical compounds in cell cultures of tobacco (Nicotiana tabacum); (2) confirmatio

291 Supplementation of ex vivo expansion cultures of Tregs with high concentrations of IL-2 or de

292 ty to human cells but were effective in cell cultures of Trypanosoma brucei brucei (EC(50) = 1-15 muM

293 tumor microenvironment (bMTM) comprising co-culture of tumor and endothelial cells in a 3D environme

294 onse assay (HDRA) based on three-dimensional culture of tumour fragments, which maintains nature tumo

295 growth dependent on Mn(II) oxidation to a co-culture of two microbial species.

296 Gompertz mineralization parameters of the co-cultures of two and three species, based on the single-s

297 eins bound to polyadenylated RNAs in primary cultures of undifferentiated spermatogonia were captured

298 Co-culture of untreated neutrophils with anti-CD3 antibody

299 Co-culture of wild-type and mutant viruses in the presence

300 s found in naive Vero cells after continuous culturing of WNV-DeltaNS1 in Vero(NS1) cells for 15 roun