ライフサイエンスコーパス: culture system

1 odel and T follicular helper (Tfh):B cell co-culture system.

2 significantly impairs myelination in our co-culture system.

3 by the cleavage event, at least in the cell culture system.

4 atients and are also produced in an in vitro culture system.

5 or of IL-10 using a human CD4(+) T cell-only culture system.

6 mentally in a novel parasite-erythrocytes co-culture system.

7 the atropselective partitioning in the cell culture system.

8 in a self-assembling, primary hepatocyte co-culture system.

9 n hampered by the lack of an efficient viral culture system.

10 u pathology in a 3D non-cell autonomous cell culture system.

11 laboratory because of limitations in the HCV culture system.

12 o induce bone resorption in an ex vivo organ culture system.

13 ghbors but inhibited by grass neighbor in co-culture system.

14 allenges the physiological relevance of this culture system.

15 en hampered by the lack of an efficient cell culture system.

16 d macrophages (J771.A1) using a Transwell co-culture system.

17 yonic stem cells in a three-dimensional (3D) culture system.

18 l, using a feeder-free and serum-free (FFSF) culture system.

19 re flagged as positive by an automated blood culture system.

20 use model of infection and a flexible tissue culture system.

21 mouse model to establish a primary podocyte culture system.

22 ous degrees, depending on the virus and cell culture system.

23 ation of Treg inhibitory activity using a co-culture system.

24 ng term in a three-dimensional (3D) organoid culture system.

25 limited by the lack of a simple, robust cell culture system.

26 zoprevir and paritaprevir in infectious cell culture systems.

27 ment in both two- and three-dimensional cell culture systems.

28 e utility of these three-dimensional primary culture systems.

29 or the development of LCs were added to both culture systems.

30 s with obesity were studied in 2D and 3D-ECM culture systems.

31 finding contradicted observations from cell culture systems.

32 and most studies have been confined to cell culture systems.

33 oduces dose-dependent SOD1 reduction in cell culture systems.

34 t liver tissue compared with two-dimensional culture systems.

35 the development of versatile and permissive culture systems.

36 n SMA model mice and human motor neuron cell culture systems.

37 n signaling modification is dependent on the culture systems.

38 ic inferences remains to be tested in tissue culture systems.

39 nal (2D) and organoid three-dimensional (3D) culture systems.

40 scuss relevant state-of-the-art dynamic cell-culture systems.

41 suspected rickettsioses using mammalian cell culture systems.

42 bone), a setting poorly recapitulated in 2D culture systems.

43 espective contributions using human in vitro culture systems.

44 Here, we present an advanced cell culture system able to mimic the dynamic environment of

45 The culture system affords 236- to 899-fold expansion over t

46 the field of stem cell biology and organoid culture systems allow the expansion and differentiation

47 ed a human peripheral blood mononuclear cell culture system and a nontransgenic mouse model in which

48 cluding the development of an efficient cell culture system and animal models for HBV investigation,

49 We developed a cell culture system and characterized HEV particles; we ident

50 Here, we present a novel microfluidic co-culture system and establish mild, moderate and severe c

51 We developed an efficient cell culture system and isolated HEV particles that were infe

52 re we outline here is applicable to any cell culture system and requires approximately 1 week to comp

53 Due to lack of an efficient cell culture system and robust small-animal model, little is

54 amine the P. falciparum cycle in an in vitro culture system and show that the parasite has molecular

55 sion are complicated by the lack of in vitro culture system and the difficulties associated with stud

56 fect in SPG11 brain development we employ 2D culture systems and 3D human brain organoids derived fro

57 n ability of ESCC cells in three-dimensional culture systems and angiogenesis assay.

58 While conventional in vitro culture systems and animal models have been used to stud

59 The results from studies conducted in cell culture systems and animals suggest that both vitamin D

60 In conclusion, we have used in vitro human culture systems and CyTOF to define the conversion of ci

61 nstrate that click-ExM is applicable in cell culture systems and for tissue imaging.

62 and possible solutions offered by novel cell culture systems and genome engineering approaches.

63 yte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomim

64 platform has been applied to both 2D and 3D culture systems and readily distinguishes between (1) cy

65 velopment, including 3D organoids, stem cell culture systems and single cell RNA sequencing, have bro

66 of ZNF410 in adult-type human erythroid cell culture systems and xenotransplantation settings diminis

67 We discuss here how genetics, new cell culture systems, and improved animal models will fuel th

68 cells in the liver, instability of in vitro culture systems, and reliance on samples from end-stage

69 ed from iPSCs, while increasingly complex co-culture systems are being developed to facilitate neuros

70 Several three-dimensional cell culture systems are currently available to create liver

71 mics within these microcolonies, new sessile culture systems are needed that sequester cells and mimi

72 bute to tumor progression and establish this culture system as a platform for studying tumor vascular

73 Here we used a cell culture system as proof of concept to show that, upon ex

74 We deployed an in vitro co-culture system based on direct contact of cancer cells w

75 In co-culture systems, BMFs secreted high levels of murine int

76 issolve Fe and Mn oxyhydroxides in bacterial culture systems, but the impact of PCA upon Fe and Mn cy

77 ts ensure the quality and reproducibility of culture systems by lowering lot-to-lot variations and th

78 neered 3D in vitro endothelial-epithelial co-culture system can be used to isolate both molecular and

79 Our in vitro culture system can be used to predict the virulence of i

80 We conclude that our 3D culture system can generate large numbers of fully funct

81 This ex vivo culture system can uniquely address the ability of CNS p

82 This primary cell-based co-culture system combined with microOCT imaging offers sig

83 viruses, affecting virus replication in the culture systems commonly used to generate and amplify va

84 A co-culture system consisting of a polarized mucosal epithel

85 In human TH1- or TH2-skewing cell culture systems, cotranscriptional R-loops (RNA/DNA dupl

86 microfabrication, microfluidics, and 3D cell culture systems could be used for the development of tum

87 e first primary human macrophage-enteroid co-culture system, defined conditions that allow for a prac

88 The microfluidic culture system described here offers an in vitro platfor

89 In a microisland culture system designed to isolate cell-cell competition

90 Using a culture system designed to mimic the physiological oxyge

91 The 3D cell culture system developed on a deformable substrate affix

92 In contrast, the transwell co-culture system displays comparatively low levels of BBB

93 However, current culture systems disrupt the inductive tissue-tissue inte

94 In human T(H) and B-cell culture systems, DNA damage-induced GDR elicited by IR o

95 o study HBx in physiologically relevant cell culture systems due to the lack of a highly specific and

96 Moreover, tri-culture system elevated blood-brain barrier gene express

97 Furthermore, this culture system enabled the assessment of contraction and

98 studies demonstrate that this primary tissue culture system enables one to study gastric tissue niche

99 The automated culture system enables the imaging of cells in the organ

100 ry human esophageal keratinocyte cell (EPC2) culture system (EPC2-air-liquid interface) was examined.

101 echanical cardiac microenvironment in tissue culture systems favorable to high-resolution cellular/su

102 (PSA)) by an in vitro primary fish gill cell culture system (FIGCS) for 24 h in artificial freshwater

103 Previously, we identified and established a culture system for a novel lineage of arenaviruses isola

104 ll-free, ligand-based activation and ex vivo culture system for CD19-specific CAR T cells.

105 a long-term feeder-free, chemically defined culture system for distal lung progenitors as organoids

106 Our findings validate a unique BCSC culture system for drug screening and offer preclinical

107 Development of a biomimetic 3D culture system for drug screening is necessary to fully

108 Together, these findings identify a cell culture system for functionally exploring the two X chro

109 we have developed a polarized 2-dimensional culture system for HAT-7 cells, a rat cell line of amelo

110 We further validate the potential of this culture system for high-throughput screening to prime an

111 sis demonstrates the utility of the HCV cell culture system for identifying novel bioactive molecules

112 d and optimized a reliable medium-throughput culture system for pig and human heart slices as a platf

113 Using an organ culture system for prostate development and Ret mutant m

114 hemically defined, and modular alveolosphere culture system for the propagation and differentiation o

115 We describe the first cell culture system for VA1, a key step necessary for the stu

116 g than MDCK cells.IMPORTANCE Robust in vitro culture systems for influenza virus are critically neede

117 Cell culture systems for prion propagation have greatly advan

118 Here, we established the first culture systems for RHV, recapitulating the intracellula

119 Included are a batch culturing system for sustained growth of subject-specifi

120 panding availability of in vitro and in vivo culture systems, genomes, transcriptomes, and transgenic

121 to society, yet the lack of reliable tissue culture systems has hampered the development of appropri

122 Three-dimensional (3D) culture systems have fueled hopes to bring about the nex

123 Recent advances in 3D culture systems have led to the generation of brain orga

124 Current three-dimensional mammary culture systems have not demonstrated concurrent prolife

125 Automated blood culture systems have shown promise as alternatives to th

126 Recently, studies in cell culture systems have shown that the nature of CRISPR/Cas

127 panese fulminant hepatitis-1 (JFH1) HCV cell culture system (HCVcc).

128 Vossen et al.'s Transwell culture system holds potential to reliably assess mechan

129 High-throughput compatible cell culture systems, however, suffer from limitations with r

130 t in an osteoblast/bone marrow macrophage co-culture system, immobilization of OPG by HS at the osteo

131 stem cell lines represent the first organoid culture system in the rat.

132 Due to the lack of a highly effective cell culture system in vitro, there is little understanding a

133 do this, we utilized a microfluidic chamber culture system in which human induced pluripotent stem c

134 Here we describe a novel culture system in which primary human HSPCs cultured und

135 NA-Seq datasets from three distinct neuronal culture systems in two activity states, enabling genome-

136 e-inactivating studies in zebrafish and cell culture systems in vitro, we show that Par3 to be essent

137 Alert Virtuo is an advanced, automated blood culture system incorporating improved automation and an

138 3D cell culture systems integrated with electrodes are now provi

139 o extend traditional 2-dimensional (2D) cell culture systems into 3D to more accurately replicate in

140 However, established co-culture systems involve brain endothelial cells, astrocy

141 This fully human keratinocyte culture system is 'regulatory friendly' and increases th

142 The alginate scaffold-based organotypic culture system is a promising, reliable, and easy system

143 Although the 2D culture system is simple and easily accessible, the cult

144 their expected effects, irrespective of the culture system, IWP2 decreased total beta-catenin while

145 This culture system led to a 73-fold increase in long-term he

146 In our in vitro T cell culture system, MART1-specific CD8 T cells were expanded

147 rmative transition of naive PSCs in a simple culture system may recapitulate essential and specific f

148 Our study demonstrates that microfluidic culture systems may offer an interesting new tool for di

149 In co-culture systems, MFs secreted high levels of IL-6, while

150 A wider range of cell culture systems might help overcome these shortcomings.

151 We have developed a co-culture system (MLH) comprising primary macrophages, hep

152 In contrast to cell culture systems, mouse models are highly favored for eva

153 vo neutrophil-NK cell-tumor cell tri-cell co-culture system, neutrophils are shown to potentially sup

154 ate presence of ameloblasts in the new 3D co-culture system of dental SCs.

155 mmunity in fish by adapting a well-described culture system of head kidney-derived macrophages of com

156 the normal mouse urothelium and an efficient culture system of human bladder cancer cells.

157 We used an organotypic culture system of human fetal testes explants called FEt

158 trophoblast (EVT) was a two-dimensional (2D) culture system of human trophoblast stem cells.

159 Using a co-culture system of primary human T cells and B lymphoma c

160 Here we present a long-term culture system of the normal mouse urothelium and an eff

161 We established a co-culture system of trigeminal ganglion sensory neurons an

162 Metabolic profiling of cells in 2D culture systems often fails to reflect the metabolism oc

163 r" (SE) regions, mainly observed in in vitro culture systems, often control production of key cell ty

164 cule PTC124 and 11 aminoglycosides in a cell culture system on four PTCs responsible for FTD or a rel

165 mpossible because there are no reported cell culture systems or in vivo models that support VA1 infec

166 this possibility, we present an in vitro co-culturing system, pancreas-on-a-chip.

167 g principles in stem cell biology, including culture systems, preclinical models, and functional asse

168 on of IAV and IBV in three human airway cell culture systems: primary human bronchial epithelial cell

169 cal study demonstrated that the Virtuo blood culture system produced results comparable to those seen

170 ative niche environment, these biomimetic co-culture systems provide a promising experimental model f

171 The automated culture system provided higher predictability of drug ab

172 This organoid culture system provides an experimental model to investi

173 This novel cell culture system provides an experimental model to underst

174 The development of in vitro culture systems quantitatively and qualitatively recapit

175 Using an in-vitro cell culture system, rat Achilles tendon fibroblasts were tre

176 (muXg) using the NASA developed rotary cell culture system (RCCS) enhanced bone resorbing osteoclast

177 go, a long-term, feeder cell- and serum-free culture system recapitulating murine epidermal architect

178 In a defined cell culture system recapitulating the extracellular matrix r

179 Cell culture systems reproducing virus replication can serve

180 e xenogeneic nature of that conventional CEA culture system restricts its use to the treatment of cri

181 nhibition of this pathway in a whole oviduct culture system resulted in a decreased embryo transport

182 Our culture system should facilitate the study of human inne

183 The robotically handled whole-tissue culture system should help advance the development of or

184 ated our experimental design on a model cell culture system showing high sensitivity and specificity,

185 In the primary cardiac fibroblast culture system, siRNA-mediated knockdown of SULF1 attenu

186 were confounded by the use of mammalian cell culture systems supplemented with fetal bovine serum.

187 al. (2019) describe an air liquid interface culture system supporting the complete life cycle of Cry

188 mple and robust human norovirus (HuNoV) cell culture system surrogate, caliciviruses still represent

189 limitations in any model system, 2D in vitro culture systems tend to be particularly restricted due t

190 portantly, by exploiting a unique epithelial culture system that allowed us to monitor alterations in

191 Here we describe an epidermal organoid culture system that allows long-term, genetically stable

192 ish a self-assembling, primary hepatocyte co-culture system that can be infected with patient-derived

193 However, there is no in vitro culture system that can capture the massive proliferatio

194 rimarily due to the lack of an in vitro cell culture system that demonstrates a deficit in replicatio

195 We established a culture system that enabled the development of cynomolgu

196 Here, we describe a co-culture system that enables the ex vivo expansion and vi

197 erformed functional studies using an ex vivo culture system that enriches for terminally differentiat

198 describe a reconfigurable microfluidic cell-culture system that facilitates the assembly of three-di

199 Here we report an organoid culture system that generates complex skin from human pl

200 ese findings identify a robust in vitro cell culture system that is amenable to addressing mechanisms

201 can be deployed in any organism or in vitro culture system that is amenable to viral transduction to

202 engineered an intestinal organotypic culture system that is capable of long-term epithelial h

203 We present a new nanoliter-scale sessile culture system that is easily implemented via microfluid

204 interactions in the gut would benefit from a culture system that maintained tissue architecture yet a

205 scribe a novel, medium-throughput biomimetic culture system that maintains viability and functionalit

206 as then applied to a reported algae-yeast co-culture system that shares typical cross-feeding feature

207 scribe how to implement a defined, xeno-free culture system that supports long-term ex vivo expansion

208 e the development of a defined, albumin-free culture system that supports the long-term ex vivo expan

209 We describe a 3D erythroid culture system that utilises a porous polyurethane (PU)

210 We devised a microfabricated organ culture system that viably preserves the normal multicel

211 A recent study reports a novel organ culture system that will enhance our ability to dissect

212 y of normal intestinal stem cells (ISCs) and culture systems that maintain them as highly immature, g

213 The development of cell culture systems that provide accurate and physiologicall

214 Organoids are multicellular culture systems that replicate tissue architecture and f

215 ibe a microenvironment mimetic in vitro cell culturing system that incorporates elements of the in vi

216 In an ex vivo culture system, the CNS requires EcI in the BBB to incor

217 numerous studies on mechanics using 2D cell culture systems, the spatial and temporal variations in

218 ue to the absence of a highly effective cell culture system, there are few reports about the interact

219 In a purified, defined culture system, these physiologic SCN ligands were suffi

220 HSV-1 could be reactivated in both culture systems; though, in contrast to 2D cultures, it

221 igh resolution lineage tracing method and 3D culture system to analyse ADM in human cells.

222 By using the culture system to analyse the intestinal absorption of 2

223 Applying this culture system to cartilage from p16tdTom reporter mice

224 sion pathway, we used an ex vivo erythrocyte culture system to decrease expression of GPA, GPB, or GP

225 We also used a neonatal rat cardiomyocyte culture system to elucidate the mechanisms underlying th

226 We further utilize an improved CD34+ culture system to engineer human red blood cells that ex

227 nical advantages of the Aplysia sensorimotor culture system to examine the role of RSK in long-term s

228 esults establish the utility of the tumoroid culture system to expand CSCs ex vivo for targeted drug

229 We have developed an ex vivo culture system to expand peripheral blood CD34(+) progen

230 We used a primary tissue culture system to generate a murine p53 null gastric tis

231 Here, using a co-culture system to interrogate the interaction between me

232 Here, we used an organoid culture system to investigate how transcription and the

233 lop a more physiologically representative co-culture system to investigate the influence of MSCs on t

234 mimetic acellular scaffold as an advanced 3D culture system to more accurately mimic the physiologica

235 report the development of a robust in vitro culture system to produce RBCs that allow the generation

236 tment or vaccine for the disease and no cell culture system to propagate the virus.

237 been well studied due to the lack of a cell culture system to propagate the virus.

238 In this study, we employed a microfluidic co-culture system to recreate important interactions in the

239 We have designed an in vitro culture system to specifically address the impact of ant

240 Using an intestinal catenary culture system to test the effects of GDNF-mediated RET

241 d the newly approved BacT/Alert Virtuo blood culture system to the BacT/Alert 3D system using 115 cli

242 Here we extend this culture system to the propagation of primary liver cance

243 sis methodologies for three-dimensional cell culture systems to improve the development and evaluatio

244 uthors used genetic deletion models and cell culture systems to investigate calcium signaling.

245 Here, we utilize several human neuronal culture systems to investigate the role of one such MAPK

246 When designing culture systems to recapitulate the in vivo oxygen envir

247 Availability of the new culture systems to study interactions between sensory ne

248 tocol can be easily applied to existing cell culture systems to study the complete HBV life cycle, in

249 er redox regulatory genes in both 2D- and 3D-culture systems, uncovering a vulnerability of spheroid

250 This study compares several adipogenic cell culture systems under a variety of conditions to assess

251 A keratinocyte cell culture system using HaCat cells was established to asse

252 ults from this paper indicated that soilless culture systems using low-intermediate salinities produc

253 sing cells, largely because the popular cell culture systems utilized for studying HCMV do not endoge

254 A bacterial-organoid co-culture system visualized the epithelial-damaging effect

255 In this study, a co-culture system was developed for innervation of intrafus

256 tion on leaf-to-root transport, a soil-based culture system was developed to monitor root system arch

257 ander cells and early infected cells in this culture system was driven by sensitization of target cel

258 To this end, a polymeric nanofiber scaffold culture system was established to develop 3D tumor organ

259 mediate IL-33 induced cytokine production, a culture system was set up to obtain pure populations of

260 This biomimetic culture system was successful in keeping human heart sli

261 EC MGIT (Becton Dickinson, Berks, UK) liquid culture system was used in all three centres for the gro

262 A bone marrow-derived MMC9 culture system was used to define IL-4-BATF signaling in

263 This culture system was used to identify a bacterial folate a

264 PDGF-BB in vasculogenesis in the 3D MM/UB co-culture system was validated by direct interference with

265 Importantly, using an ex vivo human organ culture system, we demonstrate the feasibility of human

266 Using an ex vivo colony/organoid culture system, we demonstrated that these stem/progenit

267 Using a polarized endocervical cell culture system, we determined that conditioned media (CM

268 Using a cell culture system, we established that astrocytes release b

269 her sex in a reduced two-neuron microcircuit culture system, we examined whether glutamatergic input

270 Using a multiplexed cell culture system, we find that switching between certain c

271 Using a co-culture system, we found that co-culture of QSG-7701 (hu

272 tion of our in vitro primary epithelial cell culture system, we found that prostaglandin E2 (PGE2) si

273 In a cell culture system, we found TNF to have antiviral effects i

274 l SMAD signaling inhibition in a feeder-free culture system, we have been able to expand airway basal

275 Here, using a hepatitis C virus (HCV) cell culture system, we identified neoechinulin B (NeoB), a f

276 eral proteins through an ex vivo reaggregate culture system, we identify BMPER as a novel positive re

277 Using a three-dimensional culture system, we modulate TGF, BMP, FGF, and WNT signa

278 Using two-neuron culture system, we quantified the synaptic output of ind

279 Using a rapid, clinically compliant culture system, we show that autologous BK virus-specifi

280 ced complexity in vitro macrophage-T cell co-culture system, we show that macrophage arginase-1 is th

281 Using a 3-D co-culture system, we show that macrophage-derived Gas6 act

282 Using an induced neuron (iN) culture system, we show that maternally expressed miRNAs

283 Using a novel penis culture system, we showed that exogenous estrogen direct

284 Using an ex vivo imaginal disc culture system, we showed that mitotically active sertra

285 ng a mixed neuronal lineage and microglia co-culture system, we showed that N1 stimulation changed ov

286 d brain ECM in reproducible 3D bioengineered culture systems, we demonstrate enhanced functional diff

287 Using ELISA and AXIS co-culture systems, we demonstrate that C5aR is critically

288 wound healing assays, and three-dimensional culture systems, we identified a mother centriole subdis

289 Finally, using computer simulation and cell culture systems, we provide evidence for a role of MT nu

290 In vitro primary sensory neuron culture systems were subjected to whole-transcriptome se

291 nefit industry (e.g., aquarium trade), three culture systems were tested herein with Sarcophyton glau

292 Conditioned media from these lines and co-culture systems were used to assess the dependence of Wn

293 scribed a novel, near-physiological organoid culture system, wherein primary human healthy liver cell

294 ere cultured from 2-14 days in a rotary cell culture system, which generates a simulated microgravity

295 el microfluidic based compartmentalized cell culture system, which recapitulates the connectivity of

296 Unlike other human astrovirus cell culture systems, which require addition of exogenous try

297 A successful, long-term, robust culture system will depend on a multifaceted approach co

298 cally, we established an in vitro HIV T-cell culture system with viral replication and raltegravir (R

299 were cultured for 30 days in agitation-based culture systems with 100% success rate.

300 Three-dimensional in vitro human distal lung culture systems would strongly facilitate the investigat