ライフサイエンスコーパス: cultured neuron

1 ynchronous release, or vesicle repriming, in cultured neurons.

2 tivation of NMDA-type glutamate receptors in cultured neurons.

3 to shape illumination over single somata of cultured neurons.

4 10 muM, ZIP-induced excitotoxic death of the cultured neurons.

5 a signaling scaffold protein, AKAP79/150, in cultured neurons.

6 erely reduced in cultures in gp130-deficient cultured neurons.

7 multiple mammalian cell lines and in primary cultured neurons.

8 es that recycle actively or spontaneously in cultured neurons.

9 expression from hippocampal brain slices and cultured neurons.

10 e-3 activity in defined dendritic regions of cultured neurons.

11 unoreactive puncta in dendritic processes of cultured neurons.

12 in the rat nigro-striatal projection and in cultured neurons.

13 typically measured during quantal release at cultured neurons.

14 tion caused by patients' NMDAR antibodies in cultured neurons.

15 es and changes in the metabolite profiles of cultured neurons.

16 nd reduced spine density and size in primary cultured neurons.

17 in exocytosis involved in neurite growth in cultured neurons.

18 also reduced death and apoptosis of infected cultured neurons.

19 t that AEA up-regulates Notch-1 signaling in cultured neurons.

20 media from these microglia promoted death of cultured neurons.

21 xic phenotype that causes the rapid death of cultured neurons.

22 ation of RNF167 is located on the surface of cultured neurons.

23 shRNA reduces excitatory synapse density in cultured neurons.

24 any aspect of the RIM knockout phenotype in cultured neurons.

25 actin cytoskeleton, in either HeLa cells or cultured neurons.

26 alpha4 subunit expression in hippocampus and cultured neurons.

27 lycans and participate in CSPG inhibition in cultured neurons.

28 domain and Dnmt3a RNAi blocked apoptosis of cultured neurons.

29 NMDA-mediated Ca(2+) influx and currents in cultured neurons.

30 h rate in the neurites (but not the soma) of cultured neurons.

31 ation, growth, and retraction of neurites of cultured neurons.

32 organisms such as C. elegans and dissociated cultured neurons.

33 NMDA receptor stimulation (chemical LTD) in cultured neurons.

34 th Rab11a and Rab4 in presynaptic boutons of cultured neurons.

35 turned over and display minimal toxicity to cultured neurons.

36 their effects on mitochondrial morphology in cultured neurons.

37 arborization and doubled synapse numbers in cultured neurons.

38 sed [corrected] dendritic spine head size in cultured neurons.

39 ed miniature EPSC amplitude and frequency in cultured neurons.

40 on and trafficking of glutamate receptors in cultured neurons.

41 ion decreases surface expression of NLGN1 in cultured neurons.

42 croscopy (SIM) and computational geometry in cultured neurons.

43 ial cultures exacerbated oxidative damage in cultured neurons.

44 and VAP puncta were spatially correlated in cultured neurons.

45 te mitochondrial motility and positioning in cultured neurons.

46 monitor rapid trains of action potentials in cultured neurons.

47 ipid membranes and shows enhanced binding to cultured neurons.

48 of inhibitory synapses in mouse hippocampal cultured neurons.

49 etabolites that enhance neurite outgrowth in cultured neurons.

50 ndrial and nuclear localization of RIbeta in cultured neurons.

51 e to ED peptide application also occurred in cultured neurons.

52 ranscriptional rhythms in brain explants and cultured neurons.

53 ay important roles in synaptic plasticity in cultured neurons.

54 tion, which is involved in ERK activation in cultured neurons.

55 this process are poorly understood, even in cultured neurons.

56 boosting their neuroprotective action in co-cultured neurons.

57 s glucocorticoid-induced MAO A expression in cultured neurons; 3) induction of KLF11 and translocatio

58 ly known as NR1) subunit of NMDAR causing in cultured neurons a selective and reversible internalizat

59 A) resulted in PTEN nuclear translocation in cultured neurons, a process requiring mono-ubiquitinatio

60 Furthermore, in primary cultured neurons, a proportion of UCH-L1(M) does partiti

61 o change artificial CSF (ACSF) [Na(+)](o) at cultured neurons accurately (SD<7 mM) and rapidly (<5s)

62 cellular cholesterol synthesis or content in cultured neurons affect the cleavage of amyloid precurso

63 a, and reduces Abeta production from primary cultured neurons after AAV2/1-FGF2 infection.

64 t is, kainic acid injection) and detected in cultured neurons after knockdown of Xpo1, the gene encod

65 ion of 17beta-estradiol (17betaE2) protected cultured neurons against amyloid toxicity, and its actio

66 N1 protected primary cultured neurons against toxicity and cell death trigger

67 that siRNA-mediated loss of calsyntenin-1 in cultured neurons alters APP processing to increase produ

68 Consistent with the results seen in cultured neurons, an extremely high rate of Abeta produc

69 penumbra induces autophagy and apoptosis in cultured neuron and astrocyte cell lines and that this m

70 We treated cultured neuron and astrocyte cell lines with IS for up

71 cantly reduced apoE4's neurotoxic effects in cultured neurons and a series that reduced apoE4 fragmen

72 In cultured neurons and acute brain slices, we found that m

73 Specifically, we found that, in cultured neurons and acute slices of the hippocampus, ex

74 In cultured neurons and acute slices of the olfactory bulb,

75 Both in cultured neurons and acute slices, KO of Rab11Fip5 had n

76 Using cultured neurons and an AD mouse model, we show for the

77 stimulation of central neuronal activity in cultured neurons and Ang II-induced inhibition of barore

78 tubulin tyrosination levels were observed in cultured neurons and associated with defects in axonal d

79 We tested GCaMP5s in several systems: cultured neurons and astrocytes, mouse retina, and in vi

80 In cultured neurons and brain slices, we show that Cal-Ligh

81 expression of wild-type and mutant Shank3 in cultured neurons and by binding assays in heterologous c

82 disrupted by activation of NMDA receptors in cultured neurons and by stimuli that trigger late-phase,

83 ic regions during embryogenesis, is found in cultured neurons and can modulate neuronal electrophysio

84 hich forms round, vesicle-rich inclusions in cultured neurons and causes a PD-like, l-DOPA-responsive

85 Assembled BoNT/X cleaves VAMP2 and VAMP4 in cultured neurons and causes flaccid paralysis in mice.

86 mbinant wild-type GABAAR endocytosis in both cultured neurons and COS-7 cells can be amplified by the

87 rotect against oxygen glucose deprivation in cultured neurons and did not alter the expression of LC3

88 e show that G2019S-LRRK2 expression, in both cultured neurons and dopaminergic neurons in the rat sub

89 onal protein synthesis is well recognized in cultured neurons and during development in vivo, there h

90 ssed a recombinant truncated Src fragment in cultured neurons and examined how it affects neuronal su

91 s in heterologous cells with GluN1 or in rat cultured neurons and found that surface expression corre

92 nt quantitative proximity ligation assays in cultured neurons and four independent immunoprecipitatio

93 ated by FOC can directly activate individual cultured neurons and generate intracellular Ca(2+) trans

94 Studies in cultured neurons and glia cells indicate that these chan

95 UPS activity, we found that UPS activity in cultured neurons and glia decreases in a time-dependent

96 irenz (EFV) alters mitochondrial function in cultured neurons and glial cells.

97 to Galphaolf and induce cAMP accumulation in cultured neurons and HEK-T cells.

98 In cultured neurons and hippocampal organotypic slices, syn

99 tude of inhibitory synaptic currents in both cultured neurons and hippocampal slices exposed to E2, w

100 Here we demonstrate, in cultured neurons and in acute slices, that the NMDA rece

101 induce a pathological modification of tau in cultured neurons and in bigenic mice expressing Abeta an

102 es aggregation of alpha-synuclein in primary cultured neurons and in dopaminergic neurons of the subs

103 We demonstrated in cultured neurons and in fruit fly and zebrafish larvae h

104 ccumulates and leads to neurodegeneration in cultured neurons and in mouse brain.

105 the EphrinB2 receptor in heterologous cells, cultured neurons and in mouse brain.

106 ized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and

107 ivity ("Optopatch") has been demonstrated in cultured neurons and in organotypic brain slices, but no

108 d sub-threshold membrane voltage dynamics in cultured neurons and in pyramidal cells within neocortic

109 ue 5-HT(1A) signaling of 5-HT(1A) KO mice in cultured neurons and in slices of the dorsal raphe showi

110 ecessary for its transcriptional activity in cultured neurons and in the developing brain in vivo We

111 of the endogenous biliverdin chromophore in cultured neurons and in the mammalian brain and can be r

112 lsion but not Sema3C-dependent attraction in cultured neurons and in the mouse brain.

113 rospectively identifying growing synapses in cultured neurons and in visualizing the distribution of

114 regulates excitatory synapse development in cultured neurons and in vivo, and the synaptogenic activ

115 his chimeric motor rescued axon outgrowth in cultured neurons and in vivo, firmly establishing the ro

116 -GNTI) reduced the response of DPDPE both in cultured neurons and in vivo.

117 (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in viv

118 that trigger excitatory synapse assembly in cultured neurons and influence synaptic function in vivo

119 proinflammatory cytokines that are toxic to cultured neurons and likely contribute to A-T neurodegen

120 ed synaptic loss and neuronal death in mouse cultured neurons and long-term potentiation inhibition i

121 In cultured neurons and mouse and fly brains, smFP probes a

122 In cultured neurons and nonneuronal cells, Lfc has been sho

123 th genetic and pharmacological approaches in cultured neurons and PD patient-derived cells.

124 key regulator of life or death decisions in cultured neurons and sensory cells.

125 nduced by the CSF of progressive patients in cultured neurons and suggest a critical temporal window

126 at oligomeric Abeta enhances PLD activity in cultured neurons and that this stimulatory effect does n

127 otoxins in AD, impair organelle transport in cultured neurons and transgenic mouse models.

128 lso localizes to mitochondria in HeLa cells, cultured neurons, and brain tissue.

129 ociated proteins, web-like adhesions between cultured neurons, and chromatin domains subclassified on

130 d to detect the enzyme in brain homogenates, cultured neurons, and histological sections.

131 Immunochemistry on rat brain, cultured neurons, and human embryonic kidney cells expre

132 receptors and impairing synaptic function in cultured neurons, and it prevented memory deficits and n

133 ses SMN expression in patient-derived cells, cultured neurons, and the mouse central nervous system.

134 ling was the primary cascade that attenuated cultured neuron apoptosis after growth factor withdrawal

135 nd that cytosolic proteins in axons of mouse cultured neurons are conveyed in a manner that superfici

136 response to focal ischemia in vivo and when cultured neurons are deprived of oxygen and glucose.

137 Catecholamine murine cultured neurons are more responsive to induction of MHC

138 Immunohistochemistry on rat brain and cultured neurons as well as cell-based assays were used

139 term imaging of synaptic vesicle dynamics in cultured neurons as well as in intact zebrafish.

140 rmore, LCBs induced neurite fragmentation in cultured neurons at concentrations corresponding to the

141 demonstrate a significant dIONP toxicity in cultured neurons at concentrations previously reported t

142 dative stress-mediated cell death in primary cultured neurons at nanomolar concentrations.

143 ikingly, addition of recombinant neurexin to cultured neurons at submicromolar concentrations induced

144 mpal slices, cortical synaptoneurosomes, and cultured neurons, BDNF-induced mTOR pathway activation a

145 Our results indicate that, in cultured neurons, both BDNF and EGF activate m-calpain b

146 tyrate (NaBu) induced PKCdelta expression in cultured neurons, brain slices, and animal models.

147 e slices or using a chemical LTD protocol in cultured neurons but did not affect hippocampal LTP.

148 Axonal mRNA transport is robust in cultured neurons but there has been limited evidence for

149 itic spine formation, growth and motility in cultured neurons, but its role in brain in vivo is unkno

150 been shown to block the A-type K+ current in cultured neurons, but their specificity has been questio

151 A screen for transcripts robustly induced in cultured neurons by DNA damage identified Sertad1, a Cdk

152 substantially increased the excitability of cultured neurons by increasing the spontaneous firing ra

153 We conclude that hippocampal cultured neurons can exhibit strong changes in their act

154 JNK3 deficiency reduces degeneration of cultured neurons caused by low levels of SMN.

155 icromolar concentrations of C1ql proteins to cultured neurons causes a significant decrease in synaps

156 Axotomy-induced hyper-excitability of cultured neurons coincides with elimination of inhibitor

157 Immature glutamatergic synapses in cultured neurons contain high-release probability (Pr) p

158 ion with HSV was restricted, and only 45% of cultured neurons could be productively infected with eit

159 In cultured neurons, CSF from patients, but not from contro

160 In cultured neurons, deletion of all LAR-RPTPs led to a red

161 Here, we show that, in cultured neurons derived from LRRK2 G2019S transgenic mi

162 synaptophysin function, we expressed them in cultured neurons derived from synaptophysin knock-out mi

163 in neuroblastoma cells as well as in primary cultured neurons derived from Tg2576 mice.

164 The emergence of such network structure in cultured neurons, despite a lack of external input, poin

165 Here we show that mature cortical cultured neurons display a default state characterized b

166 Furthermore, while cultured neurons display Parkin-dependent axonal mitopha

167 ian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromu

168 st NMDA, AMPA receptors, LGI1 protein) or in cultured neurons (e.g., antibodies against the GABAb rec

169 atic prion-infected mice are highly toxic to cultured neurons, exceptionally pure intact high-titer i

170 The functional networks of cultured neurons exhibit complex network properties simi

171 The NGF-cultured neurons exhibited significant depolarization, b

172 ependent arrests is based on observations in cultured neurons exposed to artificial stimuli.

173 ofen, promotes neurite elongation in primary cultured neurons exposed to axonal growth inhibitors.

174 Cultured neurons exposed to depolarizing conditions reac

175 n, in ex vivo models of demyelination and in cultured neurons exposed to glutamate and tumor necrosis

176 Ca(2+) currents and synaptic facilitation in cultured neurons expressing exogenous CaV2.1 channels.

177 and reduced branching were observed both in cultured neurons expressing shRNA for ATXN3 and in those

178 We found that cultured neurons expressing transgenic (TG) NR3A display

179 In cultured neurons, fingolimod increases BDNF levels and c

180 WT cultured neurons fired only occasional single action pot

181 teracting with endogenous alpha-synuclein in cultured neurons following delivery via nanoparticles.

182 ove outcome, we compared immature and mature cultured neurons for susceptibility to three encephaliti

183 ogenous TNF-alpha showed higher toxicity for cultured neurons from A8-deficient than for those from w

184 high spatial and temporal resolution, using cultured neurons from brains of transgenic mice overexpr

185 tivity with RG108 and procainamide protected cultured neurons from excessive DNA methylation and apop

186 Additionally, LDN/OSU-0212320 protected cultured neurons from glutamate-mediated excitotoxic inj

187 Cultured neurons from HspB1-deficient mice were more sen

188 o degradation and binds to p38 MAPK protects cultured neurons from hypoxia-reoxygenation injury and r

189 tical imaging of pHluorin-tagged proteins in cultured neurons from knock-out mice lacking each protei

190 In cultured neurons from mice that harbor an RTT patient G

191 Additional studies in cultured neurons from MPS I mice showed that elevated sp

192 t P30 showed more dendritic branching, while cultured neurons from P0 PFC extended shorter neurites t

193 Such rhythmicity is also observed in cultured neurons from the suprachiasmatic nucleus, which

194 ed the axonal transport of neurofilaments in cultured neurons from two different strains of dilute le

195 Further, immunostaining of cultured neurons from wild-type and SUMO1 knock-out mice

196 We analyzed cultured neurons from wild-type and Syn I,II,III(-/-) tr

197 tination of endogenous Bax comparing primary cultured neurons from WT and parkin KO mice and using mu

198 Moreover, in CPT1C KO cultured neurons, GluA1 synthesis after chemical long te

199 Work in cultured neurons has shown that ankyrin-G plays a key ro

200 Studies in cultured neurons have suggested that the position of the

201 in mammalian synapse function have relied on cultured neurons, heterologous expression systems, or me

202 cological elevation of membrane PI(4,5)P2 in cultured neurons impairs SV endocytosis, specifically in

203 Moreover, its expression is upregulated in cultured neurons in response to various neurotoxins, inc

204 itochondrial fragmentation and cell death in cultured neurons in vitro, in mouse substantia nigra neu

205 In cultured neurons in vitro, teneurin or FLRT alone did no

206 pression of kindlin-1, but not kindlin-2, in cultured neurons increased axon growth on an inhibitory

207 CPT1C overexpression in primary hippocampal cultured neurons increased ceramide levels, whereas in C

208 Reelin stimulation of cultured neurons induces the extension of the Golgi into

209 Neurotrophins facilitate recruitment of cultured neurons into active networks, and it is this ac

210 of endogenous NPM1 oligomerization in these cultured neurons is toxic.

211 Here, we used SGE-301 to confirm that in cultured neurons it prevented the antibody-mediated redu

212 that loss of lysosomal Cacna1a in cerebellar cultured neurons leads to a failure of lysosomes to fuse

213 ow that increased E6AP expression in primary cultured neurons leads to a reduction in dendritic branc

214 tant role in growth and neurite extension of cultured neurons, localization of laminin in the brain h

215 Studies using cultured neuron models that faithfully recapitulate the

216 zinc reversibly reduced spiking frequency of cultured neurons most likely by suppressing Kv3 channels

217 In cultured neurons, native Ric-3 levels were higher than i

218 In primary cultured neurons, NES mutations increase nuclear accumul

219 In cultured neurons, NMDA-induced superoxide production and

220 Experimental expression in cultured neurons of a short dysfunctional M1 polypeptide

221 Addition to cultured neurons of soluble teneurin-binding fragments o

222 ffects of early postnatal brain membranes or cultured neurons on MVECs were relieved significantly by

223 able and amenable to recording as individual cultured neurons or a cluster from the nervous system.

224 ripts at the RNA level after transduction of cultured neurons or after direct delivery and long-term

225 tochondria was studied either in dissociated cultured neurons or in brain slices, but not in the inta

226 Ps had no effect on synaptic connectivity in cultured neurons or in vivo, but impaired NMDA-receptor-

227 tic turnover kinetics have been performed in cultured neurons outside the context of normal circuits,

228 In cultured neurons, PDN1 fused to a fluorescent protein in

229 We found that cultured neurons prepared from NR3A knock-out (KO) mice

230 Knockdown of Miro2 in cultured neurons produced transport deficits identical t

231 ohibins or SVBP and/or inhibitor addition in cultured neurons reduced detyrosinated alpha-tubulin lev

232 Altered levels of SynDIG1 in cultured neurons result in striking changes in excitator

233 nels in both neuroblastoma cells and primary cultured neurons revealed clear genotype-phenotype corre

234 Two-photon microscopy of cultured neurons revealed large fluctuations in inner mi

235 ation of the effect of ectopic expression on cultured neurons revealed that increasing NPM1 is toxic

236 to these SGE-301 effects showed that (i) in cultured neurons SGE-301 prolonged the decay time of NMD

237 nsor for asynchronous release, we now use in cultured neurons short hairpin RNAs that suppress expres

238 Further, dissociated Ppt1(-/-) cultured neurons show extrasynaptic, diffuse calcium inf

239 In mammalian embryonic stem cells and cultured neurons, SRPK phosphorylates Ser-Arg motifs in

240 erologous expression systems and dissociated cultured neurons, studies in intact neurons revealed a s

241 dies using reporter proteins in unmyelinated cultured neurons suggest that an ankyrinG-binding motif

242 Studies in cultured neurons suggest that S-palmitoylation is requir

243 Addition of purified C3 cleavage products to cultured neurons suggested that C3b is responsible for t

244 ter stages enhanced mitochondrion numbers in cultured neurons, suggesting that CRMP5 modulated these

245 ctivity leads to spine loss from hippocampal-cultured neurons, suggesting that PKC may contribute to

246 ion of endogenous AMPA receptors (AMPARs) in cultured neurons suggests that LRRTMs maintain newly del

247 e conditional deletion of all three Mints in cultured neurons suppresses the accumulation of APP C-te

248 Cultured neurons survive well, develop extensive axonal

249 ucturally distinct and far more cytotoxic to cultured neurons than comparable LNOs made from Abeta(1-

250 We found in cultured neurons that both APP and Abeta are secreted ra

251 We show in cultured neurons that C1ql3 expression is activity depen

252 nced voltage signals excited by red light in cultured neurons that expressed paQuasAr3 (representativ

253 At synapses of cultured neurons that lack the two "neuronal" dynamins,

254 rapid depression of synaptic transmission in cultured neurons that transiently express CaV2.1 channel

255 al mGlu4 receptor agonists, and, at least in cultured neurons, the action of low concentrations of ci

256 In cultured neurons, the addition of PrP(Sc) alters cell me

257 In cultured neurons, the E183V mutation reduces CaMKIIalpha

258 In cultured neurons, these v-SNARE mutations strongly inhib

259 Acute (minutes) exposure of cultured neurons to 10 nM clothianidin, but not imidaclo

260 Here, we performed studies on cultured neurons to ascertain whether these two proteins

261 Exposure of cultured neurons to fetal plasma or to secretions from t

262 Exposure of cultured neurons to micelles composed of these ceramide

263 study, we demonstrate that acute exposure of cultured neurons to soluble Abeta oligomers induces AMPA

264 rgeted molecular and genetic approaches with cultured neurons to study cell-intrinsic host defense pa

265 to analyses of alpha-synuclein expression in cultured neurons, to examinations of the effects of vira

266 models, flies expressing Abeta and mammalian cultured neurons treated with Abeta oligomers.

267 ction was measured by the ethidium method in cultured neurons treated with oxygen-glucose deprivation

268 The pathway was confirmed by using cultured neurons treated with recombinant TNFalpha in vi

269 hermore, we confirmed the pathway using both cultured neurons treated with recombinant TNFalpha in vi

270 sease brains (Braak Stage VI), as well as in cultured neurons under conditions of oxidative stress.

271 Massive cell death occurs in cultured neurons upon depleting syntaxin-1, Munc18-1, an

272 reted by 7PA2 cells caused synapse damage in cultured neurons via a PrP(C)-dependent process.

273 In cultured neurons, VoltageSpy dyes enable robust, single-

274 In fact, reducing levels of SF3B2 in tissue-cultured neurons was effective against neurotoxicity of

275 While the density of cultured neurons was not different between PS and contro

276 In hippocampal slices and cultured neurons we also observed significant beta4 expr

277 In cultured neurons we found that mutant huntingtin causes

278 l-length and mutant GFP-tagged Navbeta4 into cultured neurons, we determined that the AIS and nodal l

279 n the present study, using mouse hippocampal cultured neurons, we evaluated the significance of cathe

280 through analysis of FOXP2 ChIP-seq data from cultured neurons, we find strong overrepresentation of a

281 In cultured neurons, we found that the HIV coat protein gp1

282 mation of hyperphosphorylated tau (P-tau) in cultured neurons, we searched for P-tau by immunohistoch

283 y sufficient to delay axonal degeneration in cultured neurons, we sought to determine whether it was

284 Cultured neurons were prepared from rat pups on postnata

285 poE4(+/+) mouse hippocampal brain tissue and cultured neurons when compared to ApoE3(+/+) counterpart

286 isease, causes dendritic and synapse loss in cultured neurons when expanded.

287 ly, we use a lentiviral expression system in cultured neurons, where we again find that eSIBR amiRNAs

288 h) enhanced voltage-gated Ca(2+) current in cultured neurons, whereas in vivo Fkbp1b knockdown by mi

289 enge previous conclusions based on work with cultured neurons, which suggested activity-dependent den

290 The protocol, exemplified here by stamping cultured neurons with adeno-associated viruses (AAVs), i

291 ankB exhibits increased axonal branching in cultured neurons with ectopic CNS axon connectivity, as

292 Treatment of cultured neurons with exogenous ceramide reverted the KO

293 Transfection of cultured neurons with human GRIN2D cDNA harboring c.1999

294 n addition, Golgi fragmentation was found in cultured neurons with hyperactivity due to prolonged blo

295 Under identical global conditions, we cultured neurons with or without local astrocyte support

296 s studies, we have shown that stimulation of cultured neurons with surrogate NCAM ligands leads to th

297 romoter was detected in approximately 90% of cultured neurons, with no preference for any neuronal su

298 f the channels triggers action potentials in cultured neurons without observable toxic effects.

299 (XBP1) in transgenic flies and in mammalian cultured neurons, yielding its active form, the transcri

300 ression, or were performed in cell lines and cultured neurons, yielding results difficult to translat