ライフサイエンスコーパス: cumulus

1 cumulus expansion in WT oocytectomized (OOX) cumulus.

2 ns, and the prevailing cloud type is shallow cumulus.

3 cause of a failure of sperm to penetrate the cumulus.

4 be regulated by capacitation and NO from the cumulus.

5 re assessed semiautomatically with software (Cumulus 4.0; University of Toronto, Toronto, Canada), an

6 Here we developed Cumulus-a cloud-based framework for analyzing large-scal

7 ciated with larger breast density values for Cumulus ABD and CumulusV but not for Volpara and Quantra

8 Variability was higher for Cumulus ABD and CumulusV than for Volpara or Quantra.

9 was measured by using an area-based method (Cumulus ABD) and three automated volumetric methods (Cum

10 9, 1.33), and 1.64% (95% CI: 1.31, 1.39) for Cumulus ABD, CumulusV, Volpara, and Quantra, respectivel

11 e models that only partially resolve shallow cumulus aggregation and cold pools.

12 hology but are equally able to penetrate the cumulus and fertilize the egg once at the site of fertil

13 pe (i.e., oocyte, granulosa cells, including cumulus and mural cells), during ovarian follicle develo

14 ection electron microscopy to examine entire cumulus and mural granulosa cells and their projections

15 imulated with progesterone (a product of the cumulus and thus encountered by sperm prior to fertiliza

16 and comprises three glomeruli, the globular cumulus and two toroidal structures.

17 tral follicle development but was low in the cumulus and virtually absent in the mural granulosa cell

18 them to overcome barriers and penetrate the cumulus and zona pellucida surrounding the egg.

19 ey must acquire more thrust to penetrate the cumulus and zona pellucida.

20 read centrally using a quantitative method (Cumulus) and its square-root metrics were analysed using

21 ere assessed by using the research software (Cumulus), and volumetric PD (VPD) and absolute dense vol

22 Cumulus cell apoptosis and matrix disassembly also occur

23 This indicates that oocytes regulate cumulus cell cholesterol biosynthesis by promoting the e

24 Cumulus cell clones initiated Oct4 expression at the cor

25 LH) induces maturational processes in oocyte-cumulus cell complexes (OCC) of preovulatory follicles t

26 hase 2 (Has2) mRNA was impaired in DM oocyte-cumulus cell complexes.

27 tes reduced levels of both KL transcripts in cumulus cell culture.

28 anges of matrix are temporally correlated to cumulus cell death.

29 ic projections to search for the oocyte, and cumulus cell differentiation results from a contact-medi

30 cells, suggesting opposing effects of FSH on cumulus cell differentiation.

31 hat Super-GDF9 can effectively promote mouse cumulus cell expansion and improve oocyte quality in vit

32 Cumulus cell expansion and resumption of meiosis with ge

33 coupled EP2 and EP4 receptor subtypes direct cumulus cell expansion and survival and oocyte meiotic m

34 In mouse granulosa cell and cumulus cell expansion assays, mouse GDF9 and human BMP1

35 strus cyclicity, estradiol biosynthesis, and cumulus cell expansion in vivo and reveal sites of actio

36 ivation in the cumulus cells, much increased cumulus cell expansion, and an accelerated severance of

37 uding follicular development, ovulation, and cumulus cell expansion.

38 dentified molecular markers for the emerging cumulus cell fate during the preantral-to-antral transit

39 We further report stage-specific oocyte-cumulus cell interactions and diverging molecular differ

40 ted structural dysgenesis with granulosa and cumulus cell layers becoming disorganised and the connec

41 Here we show that these factors control cumulus cell metabolism, particularly glycolysis and cho

42 dentified a 1605 nt cDNA sequence from mouse cumulus cell oocyte complexes (COCs) induced to expand i

43 back loop with the soma because it regulates cumulus cell replication.

44 s suggest that cAMP-elevating agents prevent cumulus cell senescence and allow them to continue to ex

45 ells, cumulus cells, and at the interface of cumulus cell transzonal projections and the oocyte.

46 etal fibroblast or terminally differentiated cumulus cell) and the recipient I2C cytoplasm, the recon

47 ct4-related genes was analyzed in individual cumulus cell-derived cloned blastocysts, only 62% correc

48 iffered between the embryonic stem cell- and cumulus cell-derived clones.

49 AMPK, was tested on denuded oocytes (DO) and cumulus cell-enclosed oocytes (CEO) maintained in meioti

50 n (AR), are potent inducers of maturation in cumulus cell-enclosed oocytes (CEO).

51 nthesized before ovulation, a process called cumulus cell-oocyte complex (COC) expansion.

52 hyaluronan-binding proteins, using parallel cumulus cell-oocyte complex (COC) extracts as positive c

53 The cumulus cell-oocyte complex (COC) matrix is an extended

54 has a critical role in the expansion of the cumulus cell-oocyte complex (COC), a process that is nec

55 dependent cross-linking of hyaluronan in the cumulus cell-oocyte complex during ovulation.

56 rthermore, the defective conformation of the cumulus cell-oocyte complex from the AR(-/-) females imp

57 Cultured TNFIP6-deficient cumulus cell-oocyte complexes also fail to expand when s

58 Cumulus cell-oocyte complexes fail to expand in TNFIP6-d

59 system, restore the expansion of Tnfip6-null cumulus cell-oocyte complexes in vitro, and rescue the f

60 Chondroitin interferes with the expansion of cumulus cell-oocyte complexes only when added with exoge

61 arides disperse cumulus cells from expanding cumulus cell-oocyte complexes with the same size specifi

62 en oocytes were microsurgically removed from cumulus cell-oocyte complexes, the isolated cumulus cell

63 rix with embedded cumulus cells, forming the cumulus cell.oocyte complex (COC) matrix.

64 us cells, lower levels were also detected in cumulus-cell-enclosed double mutant oocytes compared wit

65 cumulus cells after removal of oocytes from cumulus-cell-oocyte complexes.

66 II mouse oocytes (n = 240), with and without cumulus cells (CCs), were exposed for 4 hours to D-galac

67 ouble mutant cumulus cells, and in wild-type cumulus cells after removal of oocytes from cumulus-cell

68 GDF9 and BMP15 also regulate the function of cumulus cells after the preovulatory LH surge.

69 Embryonic keratinocytes and cumulus cells also gave rise to cloned mice.

70 Cumulus cells also promoted parthenogenetic activation t

71 layers: an outer layer of approximately 5000 cumulus cells and an inner, thick extracellular matrix,

72 acid ceramidase (AC), is expressed in human cumulus cells and follicular fluid, essential components

73 k matrix shell that is essentially devoid of cumulus cells and is enhanced upon COC expansion in vivo

74 in the development and function of both the cumulus cells and oocytes by assessing cumulus expansion

75 ntercellular metabolic cooperativity between cumulus cells and oocytes needed for energy production b

76 ls of cholesterol synthesis in double mutant cumulus cells and oocytes were partially restored by co-

77 the follicle, promoted lipid accumulation in cumulus cells and oocytes, and increased basal oocyte me

78 junction-dependent communication between the cumulus cells and the oocyte as well as intact lipid raf

79 ming disorganised and the connection between cumulus cells and the oocyte disrupted and the transcrip

80 red in vitro, prolonging the vitality of the cumulus cells and the stability of the matrix from a few

81 successful ovulation, genes expressed in the cumulus cells and those that control cumulus expansion a

82 phic factor (BDNF) secreted by granulosa and cumulus cells as an ovarian factor stimulated by the pre

83 the expression of EGF-like growth factors in cumulus cells as well as a series of molecules downstrea

84 lesterol synthesis, were highly expressed in cumulus cells compared with oocytes; and oocytes, in the

85 e transition of preantral granulosa cells to cumulus cells competent to undergo expansion.

86 SH2-B(-/-) cumulus cells do not respond to either follicle-stimulat

87 in driving the differentiation of PAGCs into cumulus cells during the preantral to antral follicle tr

88 cumulus cell-oocyte complexes, the isolated cumulus cells exhibited decreased expression levels of g

89 -/- and Gdf9+/- Bmp15-/- (double mutant, DM) cumulus cells exhibited reduced levels of both glycolysi

90 reovulatory stages but becomes restricted to cumulus cells following antrum formation.

91 l granulosa cells (PAGCs) differentiate into cumulus cells following antrum formation.

92 Hyaluronan oligosaccharides disperse cumulus cells from expanding cumulus cell-oocyte complex

93 Thus, preantral granulosa cells differ from cumulus cells in CEEF-dependent processes downstream of

94 Results showed that the role of cumulus cells in MI arrest is dichotomous.

95 at the development and/or differentiation of cumulus cells in the DM, up to the stage of the preovula

96 aged mice that ceramide is translocated from cumulus cells into the adjacent oocyte and induces germ

97 Furthermore, the impaired expansion of cumulus cells may be partially associated with altered c

98 ysis and expression of Pfkp and Ldha mRNA in cumulus cells of wild-type (WT) mice.

99 rance of cytoplasmic projections between the cumulus cells outside the zona pellucida and the oocyte

100 Hyaluronan release and dispersion of the cumulus cells progressively occur after ovulation, paral

101 bitor, inhibited Pfkp and Ldha expression in cumulus cells promoted by paracrine oocyte factors.

102 Moreover, activation of MAPK in cumulus cells requires one or more paracrine factors fro

103 Steroid hormone progesterone released by cumulus cells surrounding the egg is a potent stimulator

104 The presence of Ke 6 protein within the cumulus cells surrounding the oocyte places it in a stra

105 Cumulus cells sustain the development and fertilization

106 Cumulus cells temporarily helped to sustain MI arrest, b

107 d to identify genes more highly expressed in cumulus cells than in mural granulosa cells of mouse ant

108 emarkable interaction between the oocyte and cumulus cells that is essential for gonadotropin-induced

109 lly, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilizati

110 female mice because of the inability of the cumulus cells to assemble their hyaluronan-rich extracel

111 ient in synthesizing cholesterol and require cumulus cells to provide products of the cholesterol bio

112 tial oocyte-secreted factors or of Fshb(-/-) cumulus cells to respond.

113 re thin cytoplasmic projections that connect cumulus cells to the oocyte and are crucial for normal o

114 eased expression of Pfkp and Ldha mRNA in WT cumulus cells to the same levels as WT oocytes; however,

115 ficient mice and tested the ability of their cumulus cells to undergo mucification.

116 se decreases were prevented by culturing the cumulus cells with paracrine factors secreted by fully g

117 nteraction between the oocyte and encircling cumulus cells within a follicle, a unique venue for soma

118 Progesterone (a product of cumulus cells) also mobilises stored Ca(2+) in human spe

119 ei from somatic cells (Sertoli, neuronal and cumulus cells) taken from adult mice into enucleated mou

120 nctions are present between granulosa cells, cumulus cells, and at the interface of cumulus cell tran

121 (-/-) and Bmp15(-/-) Gdf9(+/-) double mutant cumulus cells, and in wild-type cumulus cells after remo

122 rom the Taf4b null mice had fewer (P < 0.01) cumulus cells, and the oocytes were functionally abnorma

123 Cumulus cells, but not PAGCs, are competent to undergo e

124 (HA)-rich extracellular matrix with embedded cumulus cells, forming the cumulus cell.oocyte complex (

125 duced cholesterol synthesis in double mutant cumulus cells, lower levels were also detected in cumulu

126 different kinetics of MAPK activation in the cumulus cells, much increased cumulus cell expansion, an

127 nd GDF9, promote cholesterol biosynthesis in cumulus cells, probably as compensation for oocyte defic

128 and Amh, two transcripts highly expressed in cumulus cells, suggesting opposing effects of FSH on cum

129 d oocytes, in the absence of the surrounding cumulus cells, synthesized barely detectable levels of c

130 togen-activated protein kinase (MAPK) in the cumulus cells, thus suggesting that GDF9 and BMP15 also

131 the oocyte's companion granulosa cells, the cumulus cells, was investigated using fully grown oocyte

132 ncreases in expansion-related transcripts in cumulus cells, whereas growing oocytes of preantral foll

133 and FGFs cooperate to promote glycolysis in cumulus cells.

134 a cells to essentially the same levels as in cumulus cells.

135 preantral granulosa cells differentiate into cumulus cells.

136 x3 mRNAs to 17-96% of the levels observed in cumulus cells.

137 ed embryonic stem cells and freshly isolated cumulus cells.

138 it of AMPK were detected in both oocytes and cumulus cells.

139 vels of KL-1 mRNA in mural granulosa but not cumulus cells.

140 and the oocytes were often separate from the cumulus cells.

141 an activating ligand for Adgrd1 displayed on cumulus cells.

142 of Impdh and Npr2 and elevate cGMP levels in cumulus cells.

143 lopment are provided to oocytes by companion cumulus cells.

144 s and expression of Pfkp and Ldha mRNA in WT cumulus cells.

145 paracrine factors that promote glycolysis in cumulus cells.

146 rol synthesized de novo was reduced in these cumulus cells.

147 ween the oocyte and its surrounding somatic (cumulus) cells.

148 biomass burning season showed that scattered cumulus cloud cover was reduced from 38%in clean conditi

149 ested regions have well-developed dry season cumulus cloud fields.

150 ist static energy, and (ii) the use of three cumulus cloud types (congestus, stratiform, and deep con

151 ithin a 50- to 600-meter altitude band under cumulus clouds and then glide over kilometers at low ene

152 Marine shallow cumulus clouds have long caused large uncertainty in cli

153 iding distances, birds regularly soar inside cumulus clouds to use their strong updraft, and they can

154 The response of trade cumulus clouds to warming remains a major source of unce

155 cal tropopause, ice crystal size in towering cumulus clouds, and aerosols associated with tropical bi

156 n and may eventually break up into scattered cumulus clouds, at concentrations exceeding 1,700 parts

157 ominant influence on rain initiation in warm cumulus clouds, with limited impacts of giant CCN.

158 h in situ aerosol measurements below shallow cumulus clouds.

159 Cumulus combines the power of cloud computing with impro

160 ild-type males to be detected within the egg/cumulus complex in the oviduct.

161 by comparing high-resolution observations of cumulus congestus clouds with state-of-the-art large-edd

162 importance of the representation of shallow cumulus convection.

163 This may be possible by trapping of melt by cumulus crystal growth following melt drainage from an a

164 Chemical variation across developing cumulus crystals records changes in melt composition.

165 rientation for successful penetration of the cumulus during fertilization.

166 sed on the growing follicle and the ovulated cumulus-enclosed oocyte.

167 Moreover, induction of genes involved in cumulus expansion and follicle rupture is compromised in

168 iochemical events triggered by LH, including cumulus expansion and oocyte maturation.

169 GFR expression and to restore EGFR-dependent cumulus expansion and oocyte maturation.

170 These include impairments in cumulus expansion and resumption of oocyte meiosis, as w

171 GCs were tested for their ability to undergo cumulus expansion and upregulate expansion transcripts i

172 in the cumulus cells and those that control cumulus expansion are discussed.

173 Further, we found that BMP-15 induces cumulus expansion in mouse cumulus-oocyte complexes.

174 Cumulus expansion in vitro requires secretion of cumulus

175 ed genes (Ptx3, Has2, and Ptgs2) and promote cumulus expansion in vitro, whereas mouse BMP15 and huma

176 n, DM oocytes failed to enable FSH to induce cumulus expansion in WT oocytectomized (OOX) cumulus.

177 vation of MAPK required for inducing GVB and cumulus expansion is downstream of cAMP.

178 t can be fertilized in vitro, but defects in cumulus expansion prevent fertilization in vivo.

179 This aberrant cumulus expansion was not remedied by coculture with nor

180 ar matrix surrounding the oocyte that causes cumulus expansion, and for follicle rupture in vivo.

181 human chorionic gonadotropin also stimulated cumulus expansion, and this activity was attenuated by B

182 for cyclooxygenase 2, an enzyme required for cumulus expansion, are increased.

183 We found that cumulus expansion, as well as the expression of hyaluron

184 events, including oocyte meiotic maturation, cumulus expansion, follicle wall rupture and repair, and

185 enes whose products are necessary for normal cumulus expansion, Has2 and Ptgs2.

186 h the cumulus cells and oocytes by assessing cumulus expansion, oocyte maturation, fertilization, and

187 9 and human BMP15 homodimers can up-regulate cumulus expansion-related genes (Ptx3, Has2, and Ptgs2)

188 lls may be partially associated with altered cumulus expansion-related transcripts that are regulated

189 lay delayed or reduced oocyte maturation and cumulus expansion.

190 y abolished the effect of BMP-15 in inducing cumulus expansion.

191 3 mRNA levels, all of which are required for cumulus expansion.

192 xin 3, all of which are necessary for normal cumulus expansion.

193 promoting the resumption of meiosis, governs cumulus expansion.

194 ne factors from the oocyte to induce GVB and cumulus expansion; MAPK activation alone is not sufficie

195 lus expansion in vitro requires secretion of cumulus-expansion enabling factors (CEEFs) by the oocyte

196 anization and stabilization of the expanding cumulus extracellular matrix (cECM) following an ovulato

197 P6 is a key catalyst in the formation of the cumulus extracellular matrix and indispensable for femal

198 The formation of the hyaluronan-rich cumulus extracellular matrix is crucial for female ferti

199 r the daily cycle tend to predict weak trade cumulus feedback.

200 This reduces the net rainfall of the cumulus fields, moistens the cloud layer and thus reduce

201 roup of BMP secreting mesenchymal cells (the cumulus) functions as an organizer of the dorsoventral a

202 tial EGFR-regulated events: expansion of the cumulus granulosa cell layer that encloses the oocyte an

203 abnormal polar bodies, are detached from the cumulus granulosa cell layer, and display spindle and nu

204 on by human mural granulosa cells (MGCs) and cumulus granulosa cells (CGCs) was measured by mass spec

205 r fertilization, expansion of the associated cumulus granulosa cells was inhibited.

206 preovulatory follicles were co-cultured with cumulus granulosa cells, Amh expression was increased at

207 to be required for progesterone synthesis in cumulus granulosa cells.

208 Here, we subjected oocyte-cumulus-granulosa complexes (OCGCs) derived from early a

209 to SLLP1 on in vitro fertilization with both cumulus intact and zona-free eggs, and the definition of

210 Treatment of cumulus intact oocytes with either recmSLLP1 or its anti

211 e transcription factor Pt-Ets4 is needed for cumulus integrity, dorsoventral patterning and for the a

212 he Spemann organizer, transplantation of the cumulus is able to induce a secondary axis in spiders.

213 l simulations, the daytime clearing of trade cumulus is hastened and intensified by solar heating in

214 Mucification of the cumulus layer around the oocyte is an obligatory process

215 After hormone-induced ovulation, cumulus masses were present in the oviducts of homozygou

216 y the loss of hyaluronan-linked HCs from the cumulus matrix and the appearance of oligosaccharide-lin

217 hyaluronan is a prerequisite for the correct cumulus matrix assembly and hyaluronan oligosaccharides

218 The impaired cumulus matrix formation is due to the lack of covalent

219 s indicate that appropriate formation of the cumulus matrix is essential for successful ovulation, ge

220 g the expansion of the COC and providing the cumulus matrix with its required viscoelastic properties

221 mimicking penetration into cervical mucus or cumulus matrix) was enhanced by activation of CatSper.

222 present at micromolar concentrations in the cumulus matrix, which surrounds mammalian oocytes.

223 ertile due to the lack of a correctly formed cumulus matrix.

224 ajor role in the stabilization of the murine cumulus matrix.

225 h percentage of enucleated oocytes receiving cumulus nuclei developed in vitro.

226 We benchmark Cumulus on the Human Cell Atlas Census of Immune Cells d

227 cumulated in the extracellular matrix of the cumulus oocyte complex (COC) during the process of matri

228 thma and arthritis) and in the matrix of the cumulus oocyte complex.

229 Despite similarities in the morphology of cumulus oocyte complexes (COCs) expanding in vivo and in

230 ing oocyte maturation in vivo since ovulated cumulus oocyte complexes collected from FYN (-/-) mice i

231 However, the ovulated cumulus oocyte complexes from the Taf4b null mice had fe

232 orphogenic events including expansion of the cumulus-oocyte complex (COC) matrix, oocyte maturation,

233 cle breakdown (GVBD) prior to ovulation, the cumulus-oocyte complex was markedly disrupted and the oo

234 ammatory conditions and in the matrix of the cumulus-oocyte complex, the polysaccharide hyaluronan (H

235 olism and mitochondrial efficiency in equine cumulus-oocyte complexes (COCs) during in vitro maturati

236 etach in response to progesterone and mature cumulus-oocyte complexes (COCs) was validated.

237 ," improves nuclear maturation of oocytes in cumulus-oocyte complexes derived from immature pig ovari

238 As they progress in culture, the FLI-matured cumulus-oocyte complexes display distinctly different ki

239 t surround the egg and that fertilization of cumulus-oocyte complexes from CRISP1 knockout females is

240 Granulosa cells, follicular fluid, and cumulus-oocyte complexes were collected from follicles >

241 at BMP-15 induces cumulus expansion in mouse cumulus-oocyte complexes.

242 The cumulus oophorus of large antral follicles undergoes exp

243 e oocyte and expansion (mucification) of the cumulus oophorus.

244 ains have been demonstrated in the ovulatory cumulus oophorus.

245 ger breast cancer associations were seen for Cumulus PD (OR, 1.5; 95% CI: 1.3, 1.8; processed images;

246 LIBRA PD showed strong correlations with Cumulus PD (r = 0.77-0.84) and Volpara VPD (r = 0.85-0.9

247 ccur during capacitation/transit through the cumulus, prior to any physical contact between the sperm

248 -induced cloud property changes in the trade cumulus regions of the Atlantic, which are known to disp

249 by a single radiological technologist using Cumulus software (Canto Software, Inc., San Francisco, C

250 ed all images for mammographic density using Cumulus software (Sunnybrook Health Sciences Centre, Tor

251 unknown which factors are needed to activate cumulus specific gene expression.

252 utive and inducible NOS in human oviduct and cumulus (the cellular layer investing the oocyte).

253 wet size relative to the lifetime of rising cumulus thermals.

254 ng several days along the classic stratus-to-cumulus transition zone are stratified by aerosol optica

255 ng a computer-assisted thresholding program (Cumulus), using linear regression models with generalize