ライフサイエンスコーパス: cyclohydrolase

1 s appear distinct from any characterized IMP cyclohydrolase.

2 constituents confirms their origin as PR-AMP cyclohydrolase.

3 of methylene tetrahydrofolate dehydrogenase/cyclohydrolase.

4 mino acid dopa decarboxylase (AADC), and GTP cyclohydrolase 1 (CH1) in a single transcription unit ha

5 Endothelium-targeted overexpression of GTP cyclohydrolase 1 (GCH), the rate limiting enzyme in BH4

6 We have studied the GTP-cyclohydrolase 1 (GCH-1) gene in 30 patients with the di

7 GTP cyclohydrolase 1 (GCH1) and its product tetrahydrobiopte

8 The discovery of GTP cyclohydrolase 1 (GCH1) as a genetic risk factor for PD

9 The Parkinson's disease (PD) risk gene GTP cyclohydrolase 1 (GCH1) catalyzes the rate-limiting step

10 Here we report that reduction of cardiac GTP cyclohydrolase 1 (GCH1) degradation by genetic and pharm

11 GTP cyclohydrolase 1 (GCH1) is rate limiting in the provisio

12 GTP cyclohydrolase 1 (GCH1) is the rate-limiting enzyme in t

13 rate limiting step for BH4 production is GTP Cyclohydrolase 1 (GCH1).

14 GTP-cyclohydrolase 1 (GTP-CH1) catalyzes the first step for

15 BH4, oxidised biopterins, GTP-cyclohydrolase 1 (GTPCH-1, the rate-limiting enzyme in B

16 ssociated viruses expressing human TH or GTP cyclohydrolase 1 (GTPCH1) were injected into the striatu

17 ant degradation of guanosine 5'-triphosphate cyclohydrolase 1 (GTPCH1) with consequent deficiency of

18 eno-associated viruses expressing TH and GTP cyclohydrolase 1 (GTPCH1).

19 n (a BH4 precursor) or overexpression of GTP cyclohydrolase 1 (the rate-limiting enzyme for BH4 biosy

20 ional adhesion molecule-like protein and GTP cyclohydrolase 1 feedback regulatory protein, stained in

21 ies have revealed an association between GTP cyclohydrolase 1 polymorphisms, which decrease tetrahydr

22 secondary to trangenic overexpression of GTP-cyclohydrolase 1) and reversed in wild-type mice receivi

23 GTP cyclohydrolase 1, encoded by the GCH1 gene, is an essent

24 BH4 is regulated by GTP cyclohydrolase 1, the rate-limiting enzyme in BH4 biosyn

25 of this biopterin increases with age in GTP cyclohydrolase 1-deficient hyperphenylalaninemia-1 (hph-

26 in the gene encoding guanosine triphosphate cyclohydrolase 1.

27 r BH(4) production is guanosine triphosphate cyclohydrolase-1 (GTPCH-1).

28 ference RNA (siRNA)-mediated "knockdown" GTP cyclohydrolase-1 (GTPCH1), the rate-limiting enzyme in B

29 biosynthetic enzymes (guanosine triphosphate cyclohydrolase-1 and dihydrofolate reductase).

30 e expression of an unregulated bacterial GTP cyclohydrolase-1 in plants would increase pterin biosynt

31 The expression of bacterial GTP cyclohydrolase-1 in transgenic Arabidopsis resulted in a

32 The folE gene encoding GTP cyclohydrolase-1 was cloned from Escherichia coli and in

33 e hydroxylase and guanosine-5'-tri-phosphate-cyclohydrolase-1, bilaterally into the dopamine-depleted

34 sine hydroxylase and guanosine-tri-phosphate-cyclohydrolase-1, offers a new approach to a more refine

35 We postulated that GTP cyclohydrolase-1, which catalyzes the first committed st

36 zyme methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2 (MTHFD2) is a promising therapeutic tar

37 ally methylenetetrahydrofolate dehydrogenase-cyclohydrolase 2 (MTHFD2), emerged as a top candidate in

38 e (FTL) and/or 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydroge

39 otide, between the formyltransferase and the cyclohydrolase active sites.

40 osynthesis, the AICAR transformylase and IMP cyclohydrolase activities of the bifunctional enzyme ATI

41 ncompasses both AICAR transformylase and IMP cyclohydrolase activities that are responsible for the c

42 olate-dependent AICAR transformylase and IMP cyclohydrolase activities that catalyzes the last two st

43 ide transformylase and inosine monophosphate cyclohydrolase activities, and exist as homodimers based

44 d elevations in tyrosine hydroxylase and GTP cyclohydrolase activities.

45 CH2-THF dehydrogenase and 5,10-methenyl-THF cyclohydrolase activities.

46 e activity is co-located with a methenyl-THF cyclohydrolase activity as part of bifunctional or trifu

47 at Lys137 is responsible for the increase in cyclohydrolase activity for dimeric ATIC, which was repo

48 We previously reported that deficits in GTP cyclohydrolase activity in Drosophila heterozygous for m

49 The enzyme responsible for observed IMP cyclohydrolase activity in Methanococcus jannaschii was

50 ared to test the hypothesis that the lack of cyclohydrolase activity in yMTD was due to the substitut

51 resented here, a catalytic mechanism for the cyclohydrolase activity is postulated.

52 d for site-directed mutagenesis to study the cyclohydrolase activity of this bifunctional enzyme.

53 e activity requires dimerization whereas the cyclohydrolase activity only slightly prefers the dimeri

54 The pH dependence of the PR-AMP cyclohydrolase activity shows a single titration event i

55 tant retained dehydrogenase activity, but no cyclohydrolase activity was detected.

56 f the interaction include an increase in GTP cyclohydrolase activity, with concomitant protection fro

57 nism of this enzyme and its apparent lack of cyclohydrolase activity.

58 AR) synthase and inosine monophosphate (IMP) cyclohydrolase activity.

59 oxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase).

60 arboxamide ribonucleotide transformylase IMP cyclohydrolase, an enzyme not previously known to be reg

61 that regulate biopterin bioavailability, GTP cyclohydrolase and dihydrofolate reductase exhibited a c

62 D1 (methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1).

63 nalogues and inhibitors suggest that the GTP cyclohydrolase and pyrophosphate phosphohydrolase activi

64 l protein ATIC (AICAR transformylase and IMP cyclohydrolase) and is responsible for catalyzing the pe

65 al methylene-THF dehydrogenase, methenyl-THF cyclohydrolase, and 10-formyl-THF synthetase activities.

66 10-formyl-THF synthetase, 5,10-methenyl-THF cyclohydrolase, and 5,10-methylene-THF dehydrogenase.

67 in methylenetetrahydrofolate dehydrogenase, cyclohydrolase, and formyltetrahydrofolate synthetase 1

68 ggest that both tyrosine hydroxylase and GTP cyclohydrolase are induced in a coordinate and transcrip

69 tide formyltransferase/inosine monophosphate cyclohydrolase (ATIC) and thereby slows the metabolism o

70 de ribonucleotide (AICAR) transformylase/IMP cyclohydrolase (ATIC) is a bifunctional enzyme with fola

71 arboxamide ribonucleotide transformylase/IMP cyclohydrolase (ATIC) is a bifunctional protein possessi

72 leotide transformylase/inosine monophosphate cyclohydrolase (ATIC) is responsible for catalysis of th

73 CAR) transformylase/5'-inosine monophosphate cyclohydrolase (ATIC), a bifunctional enzyme that cataly

74 oxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), a bifunctional homodimeric enzyme

75 oxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), a single enzyme in de novo purine

76 tide formyltransferase/inosine monophosphate cyclohydrolase (ATIC), SHMT1, and folate receptor (FR) a

77 e AICAR transformylase/inosine monophosphate cyclohydrolase (ATIC).

78 tide formyltransferase/inosine monophosphate cyclohydrolase (ATIC).

79 Inosine 5'-monophosphate (IMP) cyclohydrolase catalyzes the cyclization of 5-formaminoi

80 exhibits sequence homology to the type I GTP cyclohydrolases characterized by FolE, but contrary to t

81 ibits significant homology to the type I GTP cyclohydrolases characterized by FolE.

82 ssible reactions with a palm fold to include cyclohydrolase chemistry.

83 Both the dehydrogenase/cyclohydrolase (D/C) domain and the synthetase domain co

84 antly inherited guanosine triphosphate (GTP)-cyclohydrolase deficiency, otherwise known as Segawa's d

85 biogenic amine and BH4 metabolism in the GTP cyclohydrolase deficient hph-1 mouse.

86 tead, it uses a new type of thermostable GTP cyclohydrolase enzyme that produces 2-amino-5-formylamin

87 recognizable homologues of the canonical GTP cyclohydrolase enzymes that are required for riboflavin

88 h is much faster than those of canonical GTP cyclohydrolase enzymes.

89 d-product BH(4) via interaction with the GTP cyclohydrolase feedback regulatory protein (GFRP).

90 olate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase (MTHFD1

91 olate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase (MTHFD1

92 olate dehydrogenase/methenyltetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase (MTHFD1

93 r the rate-limiting BH4 synthetic enzyme GTP cyclohydrolase (GCH) became undetectable in the sweat gl

94 Endothelium-targeted overexpression of GTP cyclohydrolase (GCH) I increased levels of the endotheli

95 GTP cyclohydrolase (GCH) III from Methanocaldococcus jannasc

96 GTP-cyclohydrolase (gch1), the first enzyme in this pathway,

97 We report that GTP cyclohydrolase (GCH1), the rate-limiting enzyme for tetr

98 ces the expression of guanosine triphosphate cyclohydrolase (GCH1), the rate-limiting enzyme in pteri

99 sduction with nitric oxide synthase with GTP cyclohydrolase genes.

100 ether AMPK suppresses the degradation of GTP-cyclohydrolase (GTPCH I), a key event in vascular endoth

101 synthesis is controlled enzymatically by GTP cyclohydrolase (GTPCH), we used GTPCH-depleted mice [hyp

102 ses H4B levels and enzymatic activity of GTP cyclohydrolase (GTPCH)-1, the first step of H4B biosynth

103 pain sensitivity and chronicity, and the GTP cyclohydrolase haplotype is a marker for these traits.

104 onofunctional Methanococcus vannielii PR-AMP cyclohydrolase has been developed, and the first charact

105 5'-Phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase (HisI, PR-AMP cyclohydrolase) is a centra

106 Similarly, a GTP cyclohydrolase I (fol2) mutant of yeast (Saccharomyces c

107 similar situation in Escherichia coli: a GTP cyclohydrolase I (folE) mutant, deficient in pterin synt

108 by targeted transgenic overexpression of GTP-cyclohydrolase I (GCH), prevented hypoxia-induced pulmon

109 Guanosine triphosphate (GTP) cyclohydrolase I (GCH1) catalyzes the conversion of GTP

110 c l-amino acid decarboxylase (AADC), and GTP cyclohydrolase I (GCH1) transcription; increases striata

111 B1), carbonyl reductase (CBR1 and CBR3), GTP-cyclohydrolase I (GCH1), and 6-pyruvoyltetrahydrobiopter

112 Overexpression of GTP cyclohydrolase I (GCH1), the rate-limiting enzyme for BH

113 GTP cyclohydrolase I (GCHI) mediates the first and committin

114 GTP cyclohydrolase I (GCYH-I) is an essential Zn(2+)-depende

115 GTP cyclohydrolase I (GCYH-I) is the first enzyme of the de

116 the first enzyme of the folate pathway, GTP cyclohydrolase I (GCYH-I), encoded in Escherichia coli b

117 GTP cyclohydrolase I (GTPCH I) is the rate-limiting enzyme f

118 fer of human guanosine 5'-triphosphate (GTP) cyclohydrolase I (GTPCH I), the first and rate-limiting

119 levels, in part through the induction of GTP cyclohydrolase I (GTPCH I), the rate-limiting enzyme for

120 5812 base pairs of rat GTP cyclohydrolase I (GTPCH) 5'-flanking region were cloned

121 Recently the GTP cyclohydrolase I (GTPCH) gene was isolated as the first

122 Inhibition of GTP cyclohydrolase I (GTPCH) has been used as a selective to

123 GTP cyclohydrolase I (GTPCH) is the rate-limiting enzyme for

124 ent degradation of guanosine 5'-triphosphate cyclohydrolase I (GTPCH), a rate-limiting enzyme in the

125 me in catecholamine (CA) biosynthesis of GTP cyclohydrolase I (GTPCH), rate-limiting enzyme in biosyn

126 selective and direct-acting inhibitor of GTP cyclohydrolase I (GTPCH), the first and rate-limiting en

127 ctor alpha (TNF-alpha) without affecting GTP cyclohydrolase I (GTPCH), the rate-limiting enzyme in th

128 Expression of both iNOS and GTP cyclohydrolase I (GTPCH), the rate-limiting enzyme in th

129 4), secondary to decreased expression of GTP cyclohydrolase I (GTPCH).

130 ey enzyme involved in BH(4) synthesis is GTP-cyclohydrolase I (GTPCH-I), which is stimulated by endot

131 s controlled by guanosine triphosphate (GTP) cyclohydrolase I (GTPCHI) and its feedback regulatory pr

132 Guanosine triphosphate cyclohydrolase I (GTPCHI) is a critical enzyme in catech

133 ro-d-neopterin triphosphate catalyzed by GTP cyclohydrolase I (GTPCHI).

134 Furthermore, expression of GTP cyclohydrolase I (the rate-limiting enzyme in de novo BH

135 uman tyrosine hydroxylase (hTH) or human GTP-cyclohydrolase I [GTPCHI, the rate-limiting enzyme for t

136 n (GFRP) mediates feedback inhibition of GTP cyclohydrolase I activity by tetrahydrobiopterin and als

137 , arginine decarboxylase gene activator, GTP cyclohydrolase I and a repressor of purine biosynthesis,

138 HPS, where activities of the key enzyme GTP-cyclohydrolase I are in the normal range, but total biop

139 for the quaternary structure of GFRP and GTP cyclohydrolase I complexes.

140 increased de novo synthesis for 6BH4 via GTP-cyclohydrolase I concomitant with high levels of 6BH4, a

141 ) and the activity of guanosine triphosphate cyclohydrolase I decreased in ihMCs exposed to HG and wa

142 II increased vascular guanosine triphosphate cyclohydrolase I expression and biopterin synthesis in p

143 Conversely, both switching off GTP cyclohydrolase I expression as well as inhibiting its en

144 GTP cyclohydrolase I feedback regulatory protein (GFRP) medi

145 ylalanine through complex formation with GTP cyclohydrolase I feedback regulatory protein (GFRP).

146 olved in 6BH4 biosynthesis/recycling and GTP-cyclohydrolase I feedback regulatory protein were expres

147 iopterin bioavailability by upregulating GTP-cyclohydrolase I gene expression and activity, resulting

148 and those additionally modified with the GTP cyclohydrolase I gene indicate that BH4 is critical for

149 with fibro-blasts possessing both TH and GTP cyclohydrolase I genes displayed biochemical restoration

150 To examine further the importance of GTP cyclohydrolase I in gene therapy for PD, in vivo micro-d

151 single enzyme, as is known to occur with GTP cyclohydrolase I in the Eucarya and Bacteria, but rather

152 The activity of GTP cyclohydrolase I is inhibited by (6R)-L-erythro-5,6,7,8-

153 estoration in a rat model of PD and that GTP cyclohydrolase I is sufficient for production of BH4.

154 s with Tet-regulated expression of human GTP cyclohydrolase I to regulate intracellular BH4 availabil

155 GTP cyclohydrolase I was also required for fitness in mice a

156 Because GFRP is a pentamer and GTP cyclohydrolase I was shown here by cross-linking experim

157 ne prevented the coordinate induction of GTP cyclohydrolase I with NOS2 after exposure to interleukin

158 used a synthetic gene based on mammalian GTP cyclohydrolase I, because this enzyme is predicted to es

159 ino-6-hydroxypyrimidine, an inhibitor of GTP cyclohydrolase I, decreased endothelium-dependent vasodi

160 nzyme in the cofactor synthesis pathway, GTP cyclohydrolase I, is activated by phosphorylation and in

161 ent increase of iNOS, guanosine triphosphate cyclohydrolase I, tetrahydrobiopterin, NO formation, and

162 line by fruit-specific overexpression of GTP cyclohydrolase I, the first enzyme of pteridine synthesi

163 o fruit up to 140-fold by overexpressing GTP cyclohydrolase I, the first enzyme of pteridine synthesi

164 ro data demonstrate that NAMDA inhibited GTP cyclohydrolase I, the rate-limiting enzyme for BH4 biosy

165 tracycline-regulated expression of human GTP cyclohydrolase I, the rate-limiting enzyme in BH4 synthe

166 (X haplotype) in the GCH1 gene, encoding GTP-cyclohydrolase I, the rate-limiting enzyme in biopterin

167 irst enzyme in its biosynthetic pathway, GTP cyclohydrolase I.

168 eric GFRP associate with one molecule of GTP cyclohydrolase I.

169 timulatory complexes is equal to that of GTP cyclohydrolase I.

170 the two outer faces of the torus-shaped GTP cyclohydrolase I.

171 tA (MJ0775 gene product), a new class of GTP cyclohydrolase I.

172 g loss and suggest the importance of the GTP Cyclohydrolase I/Tetrahydrobiopterin pathway.

173 teracts directly with the zinc-dependent GTP cyclohydrolase IA, FolE (GCYH-IA).

174 ignificant (>40%) amino acid identity to GTP cyclohydrolase II (GCH II), which catalyzes the committe

175 Three GTP cyclohydrolase II homologues in the Streptomyces coelico

176 s enzyme is different than the bacterial GTP cyclohydrolase II which catalyzes both reactions.

177 ene encoding a putative dual-functioning GTP cyclohydrolase II-3,4-dihydroxy-2-butanone-4-phosphate s

178 FLU encoding the dual-functional protein GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate s

179 this enzyme confirms the involvement of GTP cyclohydrolase III (ArfA) in archaeal riboflavin and Fo

180 This activity has been reported for a GTP cyclohydrolase III protein from Methanocaldococcus janna

181 posed to begin with an archaeal-specific GTP cyclohydrolase III that hydrolyzes the imidazole ring of

182 hese compounds were potent inhibitors of IMP cyclohydrolase (IMP CHase), a second activity of the bif

183 The inosine monophosphate cyclohydrolase (IMPCH) component (residues 1-199) of the

184 but not the NGF effect, NGF also induced GTP cyclohydrolase in a cAMP-dependent manner, while the EGF

185 Sphingosine induced GTP cyclohydrolase in a protein kinase C-independent manner

186 cetylcholine, which was inhibited by the GTP-cyclohydrolase inhibitor 2,4-diamino-6-hydroxypyrimidine

187 fase substrate AICAR, as well as with an IMP cyclohydrolase inhibitor, XMP, to 1.93 A resolution.

188 methylenetetrahydrofolate dehydrogenase and cyclohydrolase involved in one-carbon metabolism.

189 GTP cyclohydrolase is composed of a highly conserved homodec

190 thetase is encoded by the purP gene, and IMP cyclohydrolase is encoded by the purO gene.

191 he induction of tyrosine hydroxylase and GTP cyclohydrolase is not coordinately regulated.

192 Mammalian GTP cyclohydrolase is subject to end-product inhibition via

193 '-monophosphate cyclohydrolase (HisI, PR-AMP cyclohydrolase) is a central enzyme in histidine biosynt

194 sine-5'-monophosphate cyclohydrolase (PR-AMP cyclohydrolase) is a Zn(2+) metalloprotein encoded by th

195 additionally modified with the gene for GTP cyclohydrolase l; an enzyme critical for BH4 synthesis.

196 thesis, QueF was proposed to be the putative cyclohydrolase-like enzyme responsible for this reaction

197 GTP is the precursor to queuosine and that a cyclohydrolase-like reaction was postulated as the initi

198 Genes encoding methenyl H(4)MPT cyclohydrolase (mch genes) were cloned and sequenced fro

199 n, we focus on the IMPCH active site and the cyclohydrolase mechanism through comparison of crystal s

200 oxamide ribonucleotide formyltransferase/IMP cyclohydrolase-mediated glucose transporter type 4 (GLUT

201 olate dehydrogenase/methenyltetrahydrofolate cyclohydrolase (MTHFD1).

202 '-phosphoribosyl) adenosine-5'-monophosphate cyclohydrolase (PR-AMP cyclohydrolase) is a Zn(2+) metal

203 active respectively as a phosphoribosyl-AMP cyclohydrolase (PRA-CH) and phosphoribosyl-ATP pyrophosp

204 Whereas canonical GTP cyclohydrolases produce this formylamino-pyrimidine nucl

205 boxamide ribonucleotideformyltransferase/IMP cyclohydrolase (PurH), an enzyme involved in de novo pur

206 noimidazolecarboxamide formyltransferase/IMP cyclohydrolase (PurH, EC 2.1.2.3/3.5.4.10).

207 Comparisons of the two types of IMP cyclohydrolase, PurO and PurH, revealed that there are n

208 an the forward rate (2.9 s(-1)), whereas the cyclohydrolase reaction is essentially unidirectional in

209 PurH but catalyzes a similar intramolecular cyclohydrolase reaction required for chromophore maturat

210 The cyclohydrolase reaction thus draws the overall bifunctio

211 THFD2 enzyme catalyzes the dehydrogenase and cyclohydrolase reactions, but the enzyme responsible for

212 leotide transformylase/inosine monophosphate cyclohydrolase, Steps 9 and 10), were studied in a polye

213 ethylene THF dehydrogenase/5,10-methenyl THF cyclohydrolase that acts upstream of 5-formyl THF format

214 re we report the identification of a new GTP cyclohydrolase that converts GTP to 7,8-dihydro-d-neopte

215 MptA is the archetype of a new class of GTP cyclohydrolases that catalyzes a series of reactions mos

216 Induction of GTP cyclohydrolase (the rate-limiting enzyme for the product

217 e activities of tyrosine hydroxylase and GTP cyclohydrolase, the rate-limiting enzymes in catecholami

218 ata, MTH1020 is confirmed as an archaeal IMP cyclohydrolase, thus designated as MthPurO.

219 0-methenyl tetrahydromethanopterin (H(4)MPT) cyclohydrolase, was constructed in vitro and recombined

220 release, and we found that the gene for GTP cyclohydrolase, which effectively regulates TH through s

221 Expression of GTP cyclohydrolase, which produces tetrahydrobiopterin (H(4)