ライフサイエンスコーパス: cysteine desulfurase

1 pathway mobilizes sulfur via SufS, a type II cysteine desulfurase.

2 and the adjacent gene, nifZ, encoding for a cysteine desulfurase.

3 cture-function properties of a mitochondrial cysteine desulfurase.

4 IscS is one of the three Escherichia coli l-cysteine desulfurases.

5 s unknown, and many archaea lack homologs of cysteine desulfurases.

6 lfur clusters is derived from L-cysteine via cysteine desulfurases.

7 layed both SufE activity (stimulating CpNifS cysteine desulfurase activity 70-fold) and quinolinate s

8 S homologs, csdA and csdB, each of which has cysteine desulfurase activity and could potentially dona

9 t proteins were similar to wild type in both cysteine desulfurase activity and sulfur transfer to Isc

10 at, of the multiple proteins in E. coli with cysteine desulfurase activity as observed by native gel

11 We postulate that the inhibition of cysteine desulfurase activity by IscX serves to reduce u

12 In Escherichia coli, the major cysteine desulfurase activity for biogenesis of iron-sul

13 urified recombinant SufE2 could activate the cysteine desulfurase activity of CpNifS 40-fold.

14 on-sulfur cluster biogenesis by limiting the cysteine desulfurase activity of NFS1, which potentiates

15 derived from the amino acid cysteine by the cysteine desulfurase activity of Nfs1.

16 complex act synergistically to modulate the cysteine desulfurase activity of SufS.

17 port that the SufE protein can stimulate the cysteine desulfurase activity of the SufS enzyme up to 8

18 esidues 376-413 (IscSDelta376-413) displayed cysteine desulfurase activity similar to the full-length

19 formation of a ternary complex with reduced cysteine desulfurase activity, and we determined a low-r

20 se data support the conclusion that, via its cysteine desulfurase activity, IscS provides the sulfur

21 sphate (PLP)-dependent enzyme with intrinsic cysteine desulfurase activity, no evidence for PLP bindi

22 PP)-conjugated acyl chain to support maximal cysteine desulfurase activity.

23 e, and had both cysteine sulfoxide lyase and cysteine desulfurase activity.

24 h as a donor of Fe(2+) and as a regulator of cysteine desulfurase activity.

25 ion, whereas ferrous iron further stimulates cysteine desulfurase activity.

26 he proteins and assayed their effect on SufS cysteine desulfurase activity.

27 in archaea is not known as many archaea lack cysteine desulfurase and an RLD of the putative ThiI.

28 terial growth, was assigned as PLP-dependent cysteine desulfurase and confirmed to be inhibited by th

29 ssense mutations exhibited similar losses of cysteine desulfurase and Fe-S cluster assembly activitie

30 inds the assembly complex and stimulates the cysteine desulfurase and iron-sulfur cluster assembly ac

31 Our analysis revealed Lecsl to be a novel cysteine desulfurase and not a type of cysteine sulfoxid

32 anistically unified by the requirement for a cysteine desulfurase and the participation of an [Fe-S]

33 ck of the gene encoding for a canonical IscS cysteine desulfurase and the presence of a short sequenc

34 ) system consisting of two components: NifS (cysteine desulfurase) and NifU (scaffold protein).

35 IscS, a cysteine desulfurase, appeared to be an exception: altho

36 Although cysteine desulfurases are conserved from bacteria to hum

37 ied the (35)S-labeled persulfide on the NFS1 cysteine desulfurase as a genuine intermediate en route

38 fE variant binds EcSufS but is not active in cysteine desulfurase assays and fails to support Fe-S cl

39 Both the cysteine desulfurase (CD) and rhodanese (Rhd) domain-con

40 persulfide intermediate in the reaction of a cysteine desulfurase (CD), CD0387 from Synechocystis sp.

41 Cysteine desulfurases (CDs) are pyridoxal-5'-phosphate (

42 ed the two constituent proteins of the human cysteine desulfurase complex (NFS1 and ISD11) separately

43 er on the scaffold protein (ISCU) involves a cysteine desulfurase complex (NFS1/ISD11) and frataxin (

44 ion into multiple biosynthetic pathways by a cysteine desulfurase complex that consists of a catalyti

45 and Lys35 residues, both bound to different cysteine desulfurase complexes NFS1-ISD11-ACP, restores

46 In vitro, the chloroplastic cysteine desulfurase CpNifS can release elemental sulfur

47 Cysteine desulfurases, designated NifS, IscS, and SufS,

48 Here we show that cysteine desulfurase encoded by the gene iscS of E. coli

49 Yfh1 has been shown to activate cysteine desulfurase enzymatic activity, whereas Yah1 su

50 athway, the pyridoxal 5'-phosphate-dependent cysteine desulfurase enzyme IscS provides sulfur to the

51 ivity pyridoxal 5'-phosphate (PLP)-dependent cysteine desulfurase enzyme that consists of catalytic (

52 d upon sulfate starvation and encapsulates a cysteine desulfurase enzyme via an N-terminal targeting

53 The cysteine desulfurase enzymes NifS and IscS provide sulfu

54 h SufE and IscU with the mutually homologous cysteine desulfurase enzymes present in their respective

55 Nfs1p is homologous to cysteine desulfurase enzymes that function in iron-sulfu

56 he catalytic efficiency (k(cat)/K(M)) of the cysteine desulfurase from 25 to 7900 M(1)s(1).

57 trate that when provided with L-cysteine and cysteine desulfurase from Escherichia coli (IscS(Ec)), L

58 D-0387), a sequence group I (NifS/IscS-like) cysteine desulfurase from Synechocystis sp. PCC 6803, by

59 und that the iscS gene, a member of the nifS cysteine desulfurase gene family, is required for 4-thio

60 eria, including Bacillus subtilis, contain a cysteine desulfurase gene sufS located adjacent to the g

61 IscS, a cysteine desulfurase implicated in the repair of Fe-S cl

62 Cysteine desulfurases in their persulfurated forms serve

63 was shown previously that impairing the NFS2 cysteine desulfurase, involved in the first step of the

64 omplex demonstrated that the activity of the cysteine desulfurase is enhanced upon encapsulation.

65 erase, receiving the sulfur donated from the cysteine desulfurase IscS and transferring it to the tar

66 The Escherichia coli L-cysteine desulfurase IscS mobilizes sulfur from L-cystei

67 following two enzymes for its synthesis: the cysteine desulfurase IscS, which forms a Cys persulfide

68 both the thiamin pathway enzyme ThiI and the cysteine desulfurase IscS.

69 y requires MnmA, Mg-ATP, l-cysteine, and the cysteine desulfurase IscS.

70 ons share strong sequence homology, e.g. the cysteine desulfurases IscS and SufS, and presumably play

71 IscX binds iron ions and interacts with the cysteine desulfurase (IscS) and the scaffold protein for

72 reduce sulfane sulfur (S(0)) produced by the cysteine desulfurase (IscS) to sulfide (S(2-)) as requir

73 The results suggest that cysteine desulfurase (IscS) together with L-cysteine can

74 scU in the presence of the iron-loaded IscA, cysteine desulfurase (IscS), and L-cysteine, demonstrati

75 orms a stable complex in vivo with the human cysteine desulfurase (ISCS), which generates the inorgan

76 ter, we identified and characterized the Mtb cysteine desulfurase (IscS; Rv3025c) and developed a nat

77 es place in the complex between IscU and the cysteine desulfurase, IscS, and our NMR studies demonstr

78 The human cysteine desulfurase, ISCS, has two isoforms, one of whi

79 The cysteine desulfurase, IscS, provides sulfur for Fe-S clu

80 S)(int) required ISC components such as Nfs1 cysteine desulfurase, Isu1/2 scaffold, and Ssq1 chaperon

81 to remove sulfur from free cysteine using a cysteine desulfurase mechanism.

82 y, serving as an allosteric activator of the cysteine desulfurase NFS1 (refs.

83 tudies showed that TUM1 interacts with the l-cysteine desulfurase NFS1 and the rhodanese-like protein

84 unexplained processing of the mitochondrial cysteine desulfurase Nfs1 in yeast.

85 ix, is a five-protein complex containing the cysteine desulfurase NFS1 that is activated by frataxin

86 r example, the assembly process requires the cysteine desulfurase Nfs1, which serves as the sulfur do

87 Frataxin interacts with Isu, iron, and the cysteine desulfurase Nfs1, which supplies sulfide, thus

88 el, that is dependent on the presence of the cysteine desulfurase Nfs1.

89 d are in the ferredoxin FDX2/fdx-2 or in the cysteine desulfurase NFS1/nfs-1 genes, resulting in amin

90 ter assembly; this complex also includes the cysteine desulfurase (Nfs1 in yeast) and the accessory p

91 In mitochondria, cysteine desulfurase (Nfs1) plays a central role in the

92 diated by the assembly complex consisting of cysteine desulfurase (NFS1), LYR protein (ISD11), acyl-c

93 rganisms, tRNA thiolation is mediated by the cysteine desulfurase, Nfs1 (IscS).

94 r modeling suggests that two subunits of the cysteine desulfurase, Nfs1, may bind symmetrically on to

95 In Saccharomyces cerevisiae, the cysteine desulfurase Nfs1p and an accessory protein, Isd

96 evidence for PLP binding or for PLP-induced cysteine desulfurase or biotin synthase activity was obs

97 Cysteine desulfurases perform pyridoxal phosphate (PLP)-

98 l-Cysteine desulfurases provide sulfur to several metaboli

99 Increasing evidence suggests that IscS, a cysteine desulfurase, provides sulfur for assembly of tr

100 e active role for SufE in promoting the SufS cysteine desulfurase reaction for Fe-S cluster assembly.

101 dimine and Cys-ketimine intermediates of the cysteine desulfurase reaction, enabling direct observati

102 NifS is a cysteine desulfurase, releasing sulfur or sulfide (depen

103 sive binding of ferredoxin and frataxin with cysteine desulfurase reported for the bacterial FeS clus

104 Nfs1, the cysteine desulfurase responsible for providing sulfur fo

105 hesized in a multistep process that utilizes cysteine desulfurases, scaffold proteins, chaperones, an

106 olves interaction of Isu with both Nfs1, the cysteine desulfurase serving as a sulfur donor, and the

107 tivity with a bacterial MnmA and its cognate cysteine desulfurase strongly suggests that the parasite

108 The Escherichia coli cysteine desulfurase SufS (EC 2.8.1.7) and its accessory

109 Deletion of the cysteine desulfurase SufS led to disruption of the apico

110 on starvation in Escherichia coli involves a cysteine desulfurase SufS.

111 The cysteine desulfurase, SufS, provides sulfur in the SUF F

112 , the cytosolic form of ISCS is a functional cysteine desulfurase that can collaborate with cytosolic

113 IscS is a widely distributed cysteine desulfurase that catalyzes the pyridoxal phosph

114 odimeric IscS protein has been shown to be a cysteine desulfurase that catalyzes the reductive conver

115 that the cytosolic form of ISCS is an active cysteine desulfurase that covalently binds 35S acquired

116 IscS from Escherichia coli is a cysteine desulfurase that has been shown to be involved

117 proteins specifically associate with IscS, a cysteine desulfurase that is proposed to sequester inorg

118 port cloning of the human homolog of NifS, a cysteine desulfurase that is proposed to supply the inor

119 as IscS, a recently characterized NifS-like cysteine desulfurase that mobilizes sulfur for the synth

120 hat decreased expression of Nfs1p, the yeast cysteine desulfurase that plays a central role in Fe-S c

121 NifS is a homodimeric cysteine desulfurase that supplies the inorganic sulfide

122 Unlike prokaryotic cysteine desulfurases, the SDA structure adopts an unexp

123 r source, and the sulfur is transferred from cysteine desulfurase through a persulfidic intermediate

124 Lecsl and that the gene Csl encoded a novel cysteine desulfurase to influence organosulfur compounds

125 ufE complex allows for comparison with other cysteine desulfurases to understand mechanisms of protec

126 Recently, a cysteine desulfurase was reported in Arabidopsis (Arabid

127 of IscS (SA1450) in S. aureus is active as a cysteine desulfurase, which enables the in vitro reconst