ライフサイエンスコーパス: cytometry

1 CD15HLA-DR (monocytes), were defined by flow cytometry.

2 50% as measured by Western blotting and flow cytometry.

3 d using extracellular flux analysis and flow cytometry.

4 and proteins were identified by imaging mass cytometry.

5 n profiles and cellular polarization by flow cytometry.

6 when assessed by next-generation flow (NGF) cytometry.

7 by histology, immunohistochemistry, and flow cytometry.

8 -selectin levels were measured by using flow cytometry.

9 ne receptor expression were analyzed by flow cytometry.

10 n situ hybridization for runx1/cmyb and flow cytometry.

11 and plasma cytokines were determined by flow-cytometry.

12 ubmandibular lymph nodes as observed by flow cytometry.

13 cells (ILC) from peripheral blood using flow cytometry.

14 ExoQuick solution and characterized by flow-cytometry.

15 xpression in individual cells simply by flow cytometry.

16 tists in the BW, which was supported by flow cytometry.

17 olecule PECAM-1 (CD31) when examined by flow cytometry.

18 Neutrophil viability was assessed by flow cytometry.

19 using multi-frequency microfluidic impedance cytometry.

20 quantitative immunohistochemistry, and flow cytometry.

21 and regulatory T cells, was explored by flow cytometry.

22 nd characterized from plasma samples by flow cytometry.

23 of nucleoprotein 1-positive cells using flow cytometry.

24 HDs exosomes were evaluated by on-bead flow cytometry.

25 g at throughputs comparable to those of flow cytometry.

26 kers of degranulation and activation by flow cytometry.

27 cells in RM from 69 HIV-negative men by flow cytometry.

28 nal wholemounts and cryosections and by flow cytometry.

29 analyses and techniques such as imaging flow cytometry.

30 aluate T and NK cells reconstitution by flow cytometry.

31 vantages of fluorescence microscopy and flow cytometry.

32 pressing Chinese hamster ovary cells by flow cytometry.

33 CD20 expression was measured by flow cytometry.

34 b to visualize granules and assessed by flow cytometry.

35 resolution using polychromatic flow and mass cytometry.

36 and C4b from serum when analyzed using flow cytometry.

37 ith high sample numbers associated with flow cytometry.

38 tranuclear) markers were assessed using flow cytometry.

39 etectable residual disease (RD; 84%) by flow cytometry.

40 e gene-knockout (GTKO), and TKO pigs by flow cytometry.

41 mster and human origin was confirmed by flow cytometry.

42 blood CD4(+) T cells was quantified by flow cytometry.

43 (ENV) or GAG peptides by multiparameter flow cytometry.

44 ation by histology, RNA sequencing, and flow cytometry.

45 d the accuracy is comparable to that of flow cytometry.

46 nd phenotypes of T cells in blood using flow cytometry.

47 mmune cell subsets using multiparameter flow cytometry.

48 ticles are also of profound interest in mass cytometry.

49 on on CD4+ T cells was determined using flow cytometry.

50 T-cell receptor repertoire analysis and mass cytometry.

51 ing intracellular cytokine staining and flow cytometry.

52 using real-time PCR, immunoblotting and flow cytometry.

53 eal-time polymerase chain reaction, and flow cytometry.

54 3 detection in immunohistochemistry and flow cytometry.

55 ion and CD107a membrane accumulation by flow cytometry.

56 ying the T-cell frequency and number by flow cytometry.

57 eal-time polymerase chain reaction, and flow cytometry.

58 38, HLADR, and/or Ki67 were assessed by flow cytometry.

59 ues were analyzed by RNA sequencing and flow cytometry.

60 nd neutrophil survival was analyzed via flow cytometry.

61 Utilizing flow cytometry, adoptive cell therapy and genetic approaches,

62 xpression on lung ILC2 were measured by flow cytometry after treatment of rTSLP, rIL-33, rTSLP + rIL-

63 (H1N1) virus challenge monitored using mass cytometry along with other clinical assessments.

64 al (heavy metal) reporter ions, such as mass cytometry (also known as CyTOF) and analogous high-dimen

65 Flow cytometry, an automated technique for measuring and sort

66 Mass cytometry analyses of splenocytes showed a significantly

67 Confocal microscopy and flow cytometry analyses showed efficient transfection efficie

68 Flow cytometry analyses showed that more than 78% (HeLa) and

69 Flow cytometry analysis demonstrated specific binding of the

70 Imaging flow cytometry analysis demonstrated that the frequency of ci

71 The flow cytometry analysis revealed that cellular uptake and ROS

72 Flow cytometry analysis showed substantially increased number

73 Flow cytometry analysis showed that the lipid accumulated in

74 e utility and power of high-dimensional mass cytometry analysis to interrogate the cellular interacti

75 The results from logic-gated flow cytometry analysis was validated with bioinformatics-bas

76 tative polymerase chain reaction assay, flow cytometry analysis, and Western blotting were applied to

77 cell transduction based on imaging and flow cytometry analysis.

78 Blood lymphocytes were quantified by flow cytometry and antigen specificity by in vitro cytokine p

79 (ILC3) and NK cells using polychromatic flow cytometry and cell stimulation assays in colon, tonsil,

80 ancreatic cancer cell populations using flow cytometry and characterized by tumor sphere formation, t

81 ne reconstitution was monitored through flow cytometry and CMV viremia was tracked via quantitative p

82 Cytotoxicity studies, clonogenic assay, flow cytometry and confocal imaging were conducted to evaluat

83 Flow cytometry and confocal microscopy detected the near-infr

84 Using immunofluorescent labeling, flow cytometry and Cre-dependent ribosomal immunoprecipitatio

85 Flow cytometry and cytokine measurements in bronchoalveolar l

86 monstrate the use of label-free imaging flow cytometry and deep learning to characterize RBC lesions.

87 Further analysis with qPCR, flow cytometry and ELISA experiments revealed that GM-CSF blo

88 Here, using flow cytometry and ELISA we show that GM-CSF induces an infla

89 eripheral blood mononuclear cells using flow cytometry and enzyme-linked immunospot assays.

90 Spodoptera frugiperda (Sf9) culture by flow cytometry and evaluating GPCR stability by size-exclusio

91 By virtue of the combined merits of flow cytometry and fluorescence microscopy, imaging flow cyto

92 onuclear cells from MOG-AAD patients by flow cytometry and found a strong antigen specific central me

93 hes and lamina propria were analyzed by flow cytometry and IgA repertoire was determined by next-gene

94 -based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shorte

95 d kidney allografts were analyzed using flow cytometry and immunohistochemical staining.

96 Flow cytometry and immunohistochemistry were used to assess e

97 rom their low-dimensional counterparts, flow cytometry and immunohistochemistry, to meet this need.

98 ecific TFH-cell responses after LAIV by flow cytometry and immunohistochemistry.

99 ients with IPEX syndrome were tested by flow cytometry and in vitro suppression assays, and the gene

100 +) T-cell responses were measured using flow cytometry and intracellular cytokine staining and compar

101 ed for the detection of TNF cleavage in flow cytometry and live-cell imaging applications.

102 sent exhibited decreased granularity by flow cytometry and marked depletion of intracytoplasmic granu

103 mes from metaphase-arrested cells using flow cytometry and perform LC-MS/MS to identify chromosome-bo

104 d ISCs and intestinal progenitors using flow cytometry and performed RNA-sequencing analysis.

105 -cell lymphoma is typically assessed by flow cytometry and plays a critical role in diagnosis, classi

106 Flow cytometry and qPCR further analyzed ex vivo the glomerul

107 the receptor's alpha-chain, analyzed by flow cytometry and quantitative RT-PCR.

108 Time-of-flight mass cytometry and RNA sequencing were used to characterize m

109 erobic (-195 +/- 15 mV) conditions, and flow cytometry and selective plating were used to quantify do

110 Through mass cytometry and single-cell RNA sequencing, we identified

111 Flow cytometry and sorted-blood-cell RNA-seq in additional pa

112 ed during flares in all 4 patients, and flow cytometry and sorted-cell RNA-seq confirmed the presence

113 ine advanced tissue dissection methods, flow cytometry and state-of-the-art proteomics to describe a

114 In addition, comprehensive phosphoflow cytometry and transcriptional profiling link the exagger

115 Cell fate assays showed that multicolor flow cytometry and transcriptional profiling successfully pre

116 by functional assays in vitro, whereas flow cytometry and transcriptomic analysis were used to asses

117 Using flow cytometry and Western blot analysis, we observed that bl

118 s are significantly reduced as shown by flow cytometry and Western blotting, using strains expressing

119 , histologic examination, IHC analysis, flow cytometry, and advanced imaging.

120 riptase quantitative PCR, intracellular flow cytometry, and ELISA.

121 -based reporter assays, genome editing, flow cytometry, and immunofluorescence microscopy.

122 Using immunoblotting, flow cytometry, and LC-MS-based glycolipid and glycan profili

123 Fluorescence microscopy, flow cytometry, and PCR were performed to determine the mitoc

124 nd CD8+ T-cell subsets were measured by flow cytometry, and prevalent diabetes cases were adjudicated

125 by immunocytochemistry, immunoblotting, flow cytometry, and real-time PCR to quantify gene expression

126 ing immunohistochemistry, polychromatic flow cytometry, and reverse transcription-PCR.

127 T cells were analyzed by flow cytometry, and serum amyloid proteins (SAA) were analyze

128 antified for T and B cell subsets using flow cytometry, and serum cytokine concentrations were measur

129 on were analyzed using high-dimensional flow cytometry, and the obtained data were compared with the

130 oblotting, immunohistochemical testing, flow cytometry, and transcriptome and cytokine profiling were

131 By using a multicolor flow cytometry approach to analyze and characterize different

132 ) in which we employ a high-dimensional mass cytometry approach to characterize innate and adaptive c

133 sional single-cell technologies such as mass cytometry are enabling time series experiments to monito

134 r, this study highlights time-of-flight mass cytometry as a reliable method for immunophenotyping the

135 Herein, we report a flow-FISH cytometry assay that detects cells expressing EBV-encode

136 We present a microsphere-based flow cytometry assay that quantifies the ability of plasma to

137 and MOG-IgG by using a live-cell-based flow cytometry assay.

138 Using a fluorescent timer and flow cytometry-assisted organelle sorting, Yau et al. develop

139 We conducted a comprehensive phenotypic flow cytometry-based longitudinal analysis of the peripheral

140 tive antibodies were detectable using a flow cytometry-based method in SARS-CoV-2-uninfected individu

141 rmits the use of immunofluorescence and flow cytometry-based methodologies to unambiguously track the

142 RNA and antigen receptor sequencing and flow cytometry-based validation.

143 We employed mass cytometry (cytometry by time of flight, CyTOF) to benchmark scMEP a

144 Cytometry by time-of-flight (CyTOF) simultaneously measu

145 ches of T cell receptor (TCR) sequencing and cytometry by time-of-flight analysis to obtain a periphe

146 Using nine large cytometry by time-of-flight mass spectrometry or mass cy

147 ted mice as assessed by high-definition mass cytometry by time-of-flight.

148 Flow cytometry (CD45.1/45.2) demonstrated abundant blood cell

149 Flow cytometry, cell culturing, and microscopy may reach thes

150 nctional properties were analyzed using flow cytometry, cell kinetic studies, co-culture with CD4 T c

151 ast epithelial cells of luminal origin, flow cytometry characterization, and genomic sequencing, we s

152 imensional immune (HDI) profiling using mass cytometry combined with other measures of vaccination an

153 osis-specific CD4 T cells detectable by flow cytometry, combined with overall elevated T-cell activat

154 Imaging flow cytometry combines the morphological information provide

155 in DLB using multiplex immunoassay and flow cytometry concomitantly.

156 Finally, flow cytometry confirmed low-level engraftment of BM cells be

157 testing, autoreactive and alloreactive flow cytometry crossmatches (FXM) using traditional FXM and o

158 Mass cytometry (CyTOF) is a technology that has revolutionise

159 by time-of-flight mass spectrometry or mass cytometry (CyTOF) studies from the open-access ImmPort d

160 We used mass cytometry (CyTOF) to characterize and compare immune cel

161 LISPOT), flow cytometry, time-of-flight mass cytometry (CyTOF), and single-cell sequencing enable onl

162 Herein, we performed the first mass cytometry (CyTOF)-based, immunophenotyping analysis of t

163 We employed mass cytometry (cytometry by time of flight, CyTOF) to benchm

164 Flow cytometry data are traditionally analyzed by (subjective

165 Analysis of flow cytometry data reconstructed a detailed map of basophil

166 ngle-cell RNA sequencing work to recent flow cytometry data suggesting a strong atheroprotective role

167 loped tools for single-cell analysis on flow cytometry data, as well.

168 Through unsupervised analyses of mass cytometry data, we show yellow fever virus-specific cCXC

169 eity and intercellular relationships in flow cytometry data.

170 dditional prerequisites when applied on flow cytometry data.

171 y, we describe the integration of multibatch cytometry datasets (iMUBAC), a flexible, scalable, and r

172 across multiple batches of high-dimensional cytometry datasets, even without technical replicates.

173 Flow cytometry demonstrated a 4.5-fold increase in CX3CR1-exp

174 Flow cytometry demonstrated decreased numbers of CD45+ cells

175 Flow cytometry demonstrated specific expression of CCR2 on mo

176 Quantitative, multicolor flow cytometry during a variety of NK cell activation conditi

177 inal endothelial cells were isolated by flow cytometry either in Tie2-GFP mice (CD31(+) CD45(-) GFP(+

178 Cytokine production was measured using flow cytometry, ELISA, RNA in situ hybridization and quantita

179 ll subsets, and DSA were measured using flow cytometry; expression of cytokines and costimulatory mol

180 ding constants from radioligand binding/flow cytometry; fast association/dissociation (~2 min)] revea

181 undance cells enabled by fluorescent droplet cytometry (FDC), an approach that uses a biomarker-speci

182 ontent multi-parameter phospho-specific flow cytometry, fluorescent cell barcoding and automated samp

183 s CD4 and tetherin was quantified using flow cytometry following transfection into an immortalized T-

184 organoid cultures and native tissues by mass cytometry for 38 markers provide a higher resolution rep

185 ss was examined with salisphere assays, flow cytometry for ALDH/CD44 (CSC markers for MEC), and Weste

186 rebellar atrophy is seen in other IGDs, flow cytometry for GPI-APs should be considered in the work-u

187 e first time, to our knowledge, imaging flow cytometry for investigating interactions of representati

188 lbumin-stimulated PBMC were analyzed by flow cytometry for presence of ovalbumin-specific regulatory

189 ts were characterized phenotypically by flow cytometry for single-cell resolution of distinct ILC sub

190 nthanide-labeled probes compatible with mass cytometry giving us the ability to monitor the activity

191 sented here suggest that large-particle flow cytometry has the potential to significantly increase ef

192 We performed multicolor flow cytometry, high-coverage single-cell RNA sequencing anal

193 We used flow cytometry, histology, and immunofluorescence to characte

194 l (HBs(hi)) using immunological assays (flow cytometry, ICS, ELISPOT).

195 ry and fluorescence microscopy, imaging flow cytometry (IFC) has become an established tool for cell

196 We have employed advanced imaging flow cytometry (iFCM) to explore the kinetics of allograft sE

197 The first application of imaging mass cytometry (IMC) with 3D cell culture enabled single-cell

198 xed ion beam imaging (MIBI) and imaging mass cytometry (IMC)), have been developed from their low-dim

199 The early analysis of progenitors using flow cytometry, immunocytochemistry, and qRT-PCR showed high

200 Flow cytometry, immunocytochemistry, immunofluorescence, West

201 , proliferation, and differentiation by flow cytometry, immunofluorescence, and organoid assays.

202 and in kidney podocytes was mapped with flow cytometry, immunoprecipitation, and trypanolysis assays

203 T cells using high-content, single-cell mass cytometry in combination with peptide-loaded MHC tetrame

204 In this study we used nanoscale flow cytometry in conjunction with an engineered FRET reporte

205 blood and cerebrospinal fluid (CSF) by flow cytometry in HIV-infected adults with cryptococcal (n =

206 Flow cytometry indicated that the IL22 was produced primarily

207 of isotopes can be employed as labels, mass cytometry is a powerful analytical technique for multipa

208 Mass cytometry (MC) is a bioanalytical technique that uses me

209 Mass cytometry (MC) measures metal isotope signals from singl

210 applicability of the different deformability cytometry methods and provides context for the interpret

211 Here, we used multidimensional flow cytometry (MFC) to prospectively screen for the presence

212 lls based only on surface markers using flow cytometry might not provide a full phenotypic picture.

213 healthy, or have SLE using single cell mass cytometry, next-generation RNA-sequencing, multiplex cyt

214 Simultaneous analysis by mass cytometry of 28 PTMs in >1 million single cells derived

215 In mice treated with Leuko-Rapa, flow cytometry of disaggregated aortic tissue revealed fewer

216 Flow cytometry of dispersed tissue fragments and serial immun

217 These include flow cytometry of endogenously expressing cells, real-time co

218 Cytometry of Reaction Rate Constant (CRRC) uses time-lap

219 Flow cytometry of the five most highly abundant peptides (EP1

220 Comprehensive flow cytometry of whole blood samples from 54 COVID-19 patien

221 Using flow cytometry on peripheral blood mononuclear cells and seru

222 Multiparametric flow cytometry on peripheral blood T cells was performed on a

223 Histology and flow cytometry on spleens and thymi from 3-week-old pups for

224 PicoP abundances and composition using flow cytometry, over a 1.5 year period.

225 Using a flow cytometry panel to assess cellular phenotypes, mRNA upta

226 Phosphoflow cytometry (phosphoflow) is a single-cell-based technique

227 the tumors were complemented with DNA image cytometry profiles, enumeration of copy numbers of eight

228 We show that imaging flow cytometry provided comprehensive population-based statis

229 l, this study demonstrates that imaging flow cytometry provides powerful means for disclosing populat

230 Flow cytometry, quantitative PCR, next-generation sequencing,

231 osis (19 with SBP) and analyzed them by flow cytometry, quantitative real-time polymerase chain react

232 iple mouse tumor and viral models using flow cytometry, quantitative reverse-transcriptase PCR (qRT-P

233 Mass cytometry reagents are probes tagged with metal isotopes

234 s measured by conventional and spectral flow cytometry reinforces the need to apply many of the recen

235 okine multiplex detection, qRT-PCR, and flow cytometry respectively.

236 ere assessed by biophotonic imaging and flow cytometry, respectively.

237 d by the MTS assay, Ki-67 staining, and flow cytometry, respectively.

238 Using a cut-off rule based on flow cytometry results of the negative control CD154-enriched

239 Flow cytometry results showed that a significantly greater pe

240 ifferences, quantification of spread by flow cytometry revealed remarkably similar spread efficiencie

241 Flow cytometry revealed that chlamydial infection induced cel

242 Immunofluorescence microscopy and flow cytometry revealed that Langerin-AhR(-/-) but not CD11c-

243 ly analyzed the brain TME landscape via flow cytometry, RNA sequencing, protein arrays, culture assay

244 tstroke inflammation was evaluated with flow cytometry, RT-PCR, MultiPlex, and immunofluorescence sta

245 Digital cytometry showed a decrease in activated and an increase

246 Results: Quantitative flow cytometry showed consistent expression of the NKp30 rece

247 Immunocytochemistry and flow cytometry showed that F302L does not impair KCNA2 subuni

248 vital microscopy via cranial window and flow cytometry showed that in the inflamed brain anti-VCAM/li

249 Interestingly, utilization of flow cytometry showed that patients with active pemphigus had

250 Mass cytometry showed that switched memory B cells were enric

251 Combining this strategy with imaging flow cytometry, single-cell RNA sequencing, and maxRNA sequen

252 d in 9 single-cell RNA sequencing and 2 mass cytometry studies.

253 and MM patients using multidimensional flow cytometry; surprisingly, we found that CD11b+CD14-CD15+C

254 Cytometry technologies are essential tools for immunolog

255 st, enzyme-linked immunospot (ELISPOT), flow cytometry, time-of-flight mass cytometry (CyTOF), and si

256 s lethal cancer, we applied DNA content flow cytometry to a series of 15 tumor samples including five

257 We used high-dimensional cytometry to analyze 125 COVID-19 patients and compare t

258 fluorophore-based confocal imaging and flow cytometry to confirm EV uptake.

259 immunophenotyping using multiparametric flow cytometry to examine peripheral immune changes under FTY

260 We performed multicolor flow cytometry to investigate CD57(+) FcepsilonRIgamma(neg) a

261 applied class II tetramer reagents and mass cytometry to investigate the ontogeny of different subse

262 Here, we use high-dimensional mass cytometry to profile protein expression and secretome of

263 T-PCR, a validated GLP-1R antibody, and flow cytometry to quantify GLP-1R promoter activity, gene exp

264 cell responses through high-dimensional flow cytometry to reveal substantial heterogeneity in both ef

265 Here, we use mass cytometry to show that metformin treatment expands a pop

266 Here, we use mass cytometry to show that two recently defined human neutro

267 Using mass cytometry to simultaneously measure multiple signalling

268 Subsequently, we used flow cytometry to validate these interactions, and a total of

269 Flow cytometry, total internal reflection fluorescence and co

270 As assessed by time-of-flight mass cytometry, total macrophages were more abundant in Tr(-/

271 expression of activation markers using flow cytometry, traditional gating-based analysis methods and

272 In this work, we used a single-cell flow-cytometry trafficking assay to quantitatively examine th

273 Because mass cytometry uses a set of lanthanide-tagged antibodies, ea

274 chain reaction; tumors were analyzed by mass cytometry using markers to detect T cells and other lymp

275 ore pipet and vesicle impact electrochemical cytometry (VIEC) at an electrode as the vesicle exits th

276 Vesicle impact electrochemical cytometry (VIEC) was used to determine the number of cat

277 easured at baseline and, in a subgroup, flow cytometry was performed at weeks 2 and 14.

278 Flow cytometry was performed to characterize CCR2+ cells.

279 Flow cytometry was used to analyze CD19(+) B cell subsets bas

280 Mass cytometry was used to characterize innate and T-cell imm

281 Flow cytometry was used to determine if lipid oxidation produ

282 Flow cytometry was used to measure IFN-gamma, IL-9, IL-13, IL

283 Furthermore, flow cytometry was used to measure the frequency of EBV-speci

284 Here, using mass cytometry, we characterise five main dILC subsets: decid

285 lls RNA sequencing and high-dimensional flow cytometry, we demonstrate that T(SCM) heterogeneity resu

286 Using flow cytometry, we determined galectin-9 expression on immune

287 the left anterior descending artery and flow cytometry, we first characterized the temporal and spati

288 nuclear phagocytes and multidimensional flow cytometry, we found that CP- and ILF-associated CD11c(+)

289 Using single-cell RNA sequencing and flow cytometry, we found that miR-155 promotes the activation

290 kinase assays, immunoprecipitation, and flow cytometry, we found that TGFbetaR signaling promotes the

291 tests, whole blood counts, and platelet flow cytometry were performed.

292 ochemistry, 3D confocal microscopy, and flow cytometry were used to characterize a novel mouse model

293 Gene expression profiling and flow cytometry were used to characterize Siglec-8 expression

294 Immunohistochemistry and flow-cytometry were used to determine the presence of pro-inf

295 otein expression were assessed by qPCR, flow cytometry, Western blotting, and immunofluorescence.

296 ifetime (FLIM) - microscopy and imaging flow cytometry with a digitally reconfigurable laser, imaging

297 solic delivery efficiency using imaging flow cytometry with cytosolic delivery validated using confoc

298 Here we coupled multiparameter flow cytometry with lesion-specific photolyases that eliminat

299 ete white blood cell count, followed by flow cytometry with multiple markers, and cytology.

300 These results demonstrate how standard mass cytometry workflows can be modified to perform high-thro