ライフサイエンスコーパス: cytosolic fraction

1 pairing such abnormal proteins mainly in the cytosolic fraction.

2 U/g wet wt or 0.07 +/- 0.03 U/mU CrK in the cytosolic fraction.

3 fraction (72%), but 28% was found within the cytosolic fraction.

4 n of nonisoprenylated Rho and Rac GTPases in cytosolic fraction.

5 duced total p130(Cas) translocation from the cytosolic fraction.

6 Stat3 exhibited a strong requirement for the cytosolic fraction.

7 tion differed depending on the source of the cytosolic fraction.

8 nd Bax were predominantly present in a crude cytosolic fraction.

9 to the NM concomitant with a decrease in the cytosolic fraction.

10 Pex19p localizes predominantly to the cytosolic fraction.

11 uclear fraction and approximately 10% in the cytosolic fraction.

12 pressed low levels that were confined to the cytosolic fraction.

13 he preponderance of p27Kip1 was found in the cytosolic fraction.

14 not result in a concomitant decrease in the cytosolic fraction.

15 type and DeltaF508 CFTR can be found in the cytosolic fraction.

16 lithium very little tau was detected in the cytosolic fraction.

17 ble of cleaving Bax which is absent from the cytosolic fraction.

18 imately 50-kDa FLAG-NMT predominantly in the cytosolic fraction.

19 ilized cells was as efficient as that of the cytosolic fraction.

20 ction (15% of protein; 8% of activity) and a cytosolic fraction.

21 ted by a reaction that depended on ATP and a cytosolic fraction.

22 ant forms were predominantly detected in the cytosolic fraction.

23 of ameloblast cell lines in addition to the cytosolic fraction.

24 o iHg ((GS)(2)-Hg) and MeHg (GS-MeHg) in the cytosolic fraction.

25 However, both increased 5LO levels in the cytosolic fraction.

26 on the amount of Pt bound to proteins in the cytosolic fraction.

27 er in the mitochondrial fraction than in the cytosolic fraction.

28 GF stimulated synthesis of 15(S)-HETE in the cytosolic fraction.

29 stern blot analysis was localized within the cytosolic fraction.

30 as redistributed from the particulate to the cytosolic fraction.

31 rendered mtTrx to be solely expressed in the cytosolic fraction.

32 vered essentially equally in particulate and cytosolic fractions.

33 the 68-kDa polypeptide was localized to the cytosolic fractions.

34 ed in membrane-enriched fractions but not in cytosolic fractions.

35 nificantly elevated in both the membrane and cytosolic fractions.

36 increased total PKC activity in membrane and cytosolic fractions.

37 ticulate fraction, but not in the nuclear or cytosolic fractions.

38 e nuclear fraction, not with the membrane or cytosolic fractions.

39 seeds occurred rapidly in both vesicular and cytosolic fractions.

40 in membrane fractions while increasing it in cytosolic fractions.

41 rylated GRK5 is found in plasma membrane and cytosolic fractions.

42 and MsrA was detected in both cell wall and cytosolic fractions.

43 ions and concurrent decreases in PKCalpha in cytosolic fractions.

44 B-crystallin, and MKBP were predominantly in cytosolic fractions.

45 beta-mercaptoethanol cell wall extracts and cytosolic fractions.

46 P3 membrane-enriched fractions and little in cytosolic fractions.

47 as separated at 45,000g into particulate and cytosolic fractions.

48 ent period and partitioned into nuclear, not cytosolic, fractions.

49 o date (54%) compared with proteins from the cytosolic fraction (35%).

50 idence of lineage-specific proteins than the cytosolic fraction (57% and 73% homologue incidence resp

51 a Km for cAMP of 3.3 +/- 0.6 microM, and the cytosolic fraction a Km of 5.4 +/- 2.8 microM.

52 eased in particulate fractions compared with cytosolic fractions after habituation training and was m

53 , the preferential processing of ICAD in the cytosolic fraction allows the translocation of DFF40/CAD

54 h a 50-fold increase in PLA2 activity in the cytosolic fraction, an increase in agonist-stimulated ar

55 ealed a band (15 kDa) of cytochrome c in the cytosolic fraction and a corresponding decrease in the m

56 tive Gbeta5 protein complex from the retinal cytosolic fraction and identified the protein tightly as

57 tion decreased betaPix/AR association in the cytosolic fraction and increased binding of AR to betaPi

58 rome c release from the mitochondrial to the cytosolic fraction and increased cleavage of caspases 3

59 OSBP1 is detected mainly in the soluble or cytosolic fraction and nuclei whereas OSBP2 is detected

60 nd atorvastatin increased COX2 levels in the cytosolic fraction and the membranous fraction.

61 uitinated proteins in both nucleoprotein and cytosolic fractions and decreased unconjugated ubiquitin

62 observation that pgp3 was highly enriched in cytosolic fractions and had a minimal presence in the in

63 Immunoblot analysis of nuclear and cytosolic fractions and immunohistochemistry revealed th

64 CRHSP-28 was present in a 60-kDa complex in cytosolic fractions and in a high molecular mass complex

65 etermine 4F binding IRF5 in endothelial cell cytosolic fractions and to confirm biolayer interferomet

66 ee system (Sph kinase obtained from platelet cytosolic fractions) and much stronger than that by DL-t

67 00 x g soluble and membrane fractions of the cytosolic fraction, and 3) with no significant effects o

68 and involucrin proteins were detected in the cytosolic fraction, and involucrin levels increased afte

69 tions, the betaII isoform was reduced in the cytosolic fraction, and the betaI and gamma isoforms wer

70 al partitioning resulted in a loosely bound (cytosolic) fraction, and a tightly bound (nuclear) fract

71 nate in both a light membrane fraction and a cytosolic fraction; and (iii) MTMR13 membrane associatio

72 an RFC antiserum recognized two bands in the cytosolic fraction (approximately 60 kDa and approximate

73 p532 has been located in the cytosolic fraction as well as in the Golgi apparatus.

74 d the K(+)-induced loss of [14C]ACh from the cytosolic fraction as well as the K(+)-induced gain of [

75 ctions showed selective association with the cytosolic fraction, as documented by copurification with

76 We determined that PR in the cytosolic fraction associated with hsp90 and p23.

77 Further analysis of this cytosolic fraction at 100,000 x g indicates that the COX

78 iol increased P-STAT3 in the ischemic cortex cytosolic fraction at 3 h after MCAO without affecting T

79 nfection and was highly enriched in the host cytosolic fraction but absent in the purified chlamydia

80 Most of the protein was localized in the cytosolic fraction, but a small fraction was detected in

81 tate, RhoA was observed predominantly in the cytosolic fraction, but it was translocated in part to t

82 tate, RhoA was observed predominantly in the cytosolic fraction, but it was translocated in part to t

83 pp37 was purified from the cytosolic fraction by anti-Tyr(P) affinity chromatograph

84 d purified 71.2-fold to homogeneity from the cytosolic fraction by chromatography with concanavalin A

85 ated pool of p190RhoGAP between membrane and cytosolic fractions by a mechanism that requires direct

86 crosomal fractions by inhibiting PDE3 and in cytosolic fractions by inhibiting both PDE3 and PDE1.

87 Western blot analysis of nuclear and cytosolic fractions confirmed that there was no transloc

88 not observed when Ca(2+) was applied to the cytosolic fraction containing sarcoplasmic reticulum.

89 The activity present in the cytosolic fraction could be depleted with antibodies to

90 ectrophoretic mobility shift assay using the cytosolic fractions demonstrated that this flavone elici

91 e ligand-binding assays, using [(3)H]TCDD in cytosolic fractions, demonstrated that 3'-methoxy-4'-nit

92 port along microtubules and diffusion in the cytosolic fraction exhibit anomalous (nonFickian) behavi

93 applied a rapid filtration method to obtain cytosolic fractions for Western blotting.

94 The cytosolic fraction from lung and bronchial alveolar lava

95 Finally, gel filtration analysis of cytosolic fractions from 293 cells that were coexpressin

96 n phosphorylation, tubulin was isolated from cytosolic fractions from anti-IgM-activated B-cells (DT4

97 , synaptic vesicle-enriched and synaptosomal cytosolic fractions from rat brain, as well as the mitoc

98 ivity profiles in synaptosomal, nuclear, and cytosolic fractions from rat frontal cortex homogenate u

99 glucose, was decreased 88% in dialyzed liver cytosolic fractions from selenium-deficient rats.

100 Screening of cytosolic fractions from several cell types and rat brai

101 Cytosolic fractions from SNAP-treated thymocytes cleaved

102 The particulate and cytosolic fractions from the double disruption (lpp1Delt

103 s; however, cytochrome c was not detected in cytosolic fractions from these cells.

104 veloped and used to screen a large number of cytosolic fractions from various tissues.

105 the biochemical analysis of the nuclear and cytosolic fractions further confirmed the nuclear transl

106 The enzyme, which was recovered in the cytosolic fraction, has a neutral pH optimum, and dithio

107 , whereas no aggregation was observed in the cytosolic fraction in a 3-day period.

108 shifted from the membranous fraction to the cytosolic fraction in the cdc43 mutants, and levels of t

109 of NF-kappaB levels in both the nuclear and cytosolic fractions in a time-dependent manner.

110 -type formin is detected in both nuclear and cytosolic fractions; in contrast, the truncated formins

111 y and sedimentation velocity analyses of the cytosolic fraction indicate that HPS1 and HPS4 form a mo

112 levels of cytochrome c were increased in the cytosolic fraction, indicating a decline in mitochondria

113 Partitioning of kinesin between vesicle and cytosolic fractions is highly sensitive to buffer compos

114 -9 and caspase-3 was markedly decreased in a cytosolic fraction isolated from HeLa cells with reduced

115 Titration of the 5'-UTR with a cytosolic fraction isolated from HuH-7 grown in the pres

116 magnesium was maximally demonstrable in the cytosolic fraction; it was markedly reduced by okadaic a

117 The presence of the enzyme in the cytosolic fraction, its dependence on a reducing environ

118 lied by the isolation of the cFTase from the cytosolic fraction, its high affinity for its substrates

119 lpha-synuclein is found predominantly in the cytosolic fractions, membrane-bound alpha-synuclein has

120 icase in a cell extract was inhibited by the cytosolic fraction obtained from gef1Delta yeast.

121 d -epsilon isoforms between the membrane and cytosolic fractions occurred in response to 100 pM AVP.

122 rajectories of microspheres migrating in the cytosolic fraction of a cellular extract.

123 Applying this approach to the crude cytosolic fraction of Arabidopsis thaliana leaves using

124 The cytosolic fraction of both cell types stimulated the act

125 of protein substrates for this enzyme in the cytosolic fraction of brain, heart, liver, and erythrocy

126 d beta-cell death, that is attributed to the cytosolic fraction of C3.

127 ntage of Ras-GRF from the particulate to the cytosolic fraction of cells and renders the protein inse

128 spho-FoxO3a (inactive form) increased in the cytosolic fraction of cells treated with iron in a PI3K-

129 have detected caspases -8 and -3 only in the cytosolic fraction of cells, but caspases -2, -7, and -9

130 The latter two activities are present in a cytosolic fraction of Crithidia fasciculata, which is ea

131 three different column chromatographies of a cytosolic fraction of frog photoreceptor outer segments,

132 the endonuclease is poorly expressed in the cytosolic fraction of healthy SK-N-AS cells.

133 The cytosolic fraction of hT17M Rho retinal cells was used t

134 e-stabilized nNOS.hsp90 heterocomplex in the cytosolic fraction of human embryonic kidney 293 cells s

135 is catalyzed by an enzyme(s) located in the cytosolic fraction of kidneys.

136 tate transaminases, and DNA fragments in the cytosolic fraction of liver homogenates were higher in c

137 e mitochondrial swelling and increase in the cytosolic fraction of mitochondrial intermembrane protei

138 coli and COS-1 cells, respectively, and the cytosolic fraction of mononuclear leukocytes from NAT1*4

139 B56 epsilon PP2A subunits is observed in the cytosolic fraction of mouse retina, the phosducin dephos

140 raction with a corresponding decrease in the cytosolic fraction of myocardial lysates from hearts sub

141 Levels of TGase activity in the cytosolic fraction of old chondrocytes were 7-fold highe

142 of CaMKII-beta but not alpha protein in the cytosolic fraction of Pb2+-exposed rats.

143 ule proteins through a mechanism linked to a cytosolic fraction of phospholipase CB1 (PLCB1).

144 as purified approximately 1275-fold from the cytosolic fraction of pig kidney, and the properties of

145 , GFPP) was purified about 560-fold from the cytosolic fraction of pig kidney.

146 It is concluded that the cytosolic fraction of porcine aorta contains a protein f

147 Recent evidence suggests a cytosolic fraction of PrP (cyPrP) functions either as an

148 % proteome coverage, are identified from the cytosolic fraction of S. oneidensis, while requiring <50

149 ntrast, a 38-kDa species was detected in the cytosolic fraction of SY5Y cells.

150 pha was translocated from the particulate to cytosolic fraction of TF-1 cells within 5 minutes of tre

151 Stimulating with IP(6) increases the cytosolic fraction of the activated BTK.

152 fragment of PIDD (PIDD-CC) increased in the cytosolic fraction of the hippocampal CA1 subregion and

153 tly elevated in the membrane, but not in the cytosolic fraction of the hippocampus of AD subjects.

154 nto 5-oxo-ETE was found predominantly in the cytosolic fraction of the macrophage, with an approximat

155 restricted eNOS mutant (myr-) as well as the cytosolic fraction of the palmitoylation-deficient eNOS

156 both enzymes are associated with the soluble cytosolic fraction of the parasite, with no evidence tha

157 The cytosolic fraction of the tumor suppressor p53 activates

158 t membrane-associated and thus appear in the cytosolic fraction of thymocyte and splenocyte extracts.

159 also induced the appearance in membrane and cytosolic fractions of a PKCalpha-immunoreactive band th

160 The cytosolic fractions of all ABPs are unexpectedly high (0

161 nd syntaxin 2 were found in the membrane and cytosolic fractions of cells, whereas syntaxin 4 was det

162 in mitochondria, and to a lesser extent, in cytosolic fractions of cultured astrocytes.

163 f the BH3 domains requires both membrane and cytosolic fractions of cytoplasm, involves the release o

164 ate levels of Hsp70 in the mitochondrial and cytosolic fractions of different tissues of diabetic rat

165 ncreased in membrane fractions and decreased cytosolic fractions of GK soleus muscles.

166 TIP47 was found primarily in the cytosolic fractions of HeLa cells and murine MA10 Leydig

167 e evidence for the presence of HIF-1alpha in cytosolic fractions of hypoxic cells which physically in

168 Immunoblots from membrane and cytosolic fractions of isolated PF cells revealed conven

169 GFbeta-IIR was detected both in membrane and cytosolic fractions of MDA-231 tumor cells as well as in

170 sed PKCalpha and PKC in the membrane and the cytosolic fractions of mice hippocampus.

171 levels of phospho-PKC-zeta were lower in the cytosolic fractions of OPN knock-out mice hearts as comp

172 uccinyl-CoA synthetase activity was found in cytosolic fractions of pancreatic islets.

173 Q was only observed in microsomal but not in cytosolic fractions of rainbow trout (O. mykiss) liver S

174 chelerythrine stimulated PKC activity in the cytosolic fractions of rat and mouse brain in concentrat

175 edly, FOX activity was noted in membrane and cytosolic fractions of rat enterocytes.

176 CC1 protein levels in the plasma membrane or cytosolic fractions of spinal cords.

177 d to be degraded in both the particulate and cytosolic fractions of TF-1 cells after treatment with a

178 determine the capacity of proteins found in cytosolic fractions of the bovine adrenal medulla to bin

179 ty was associated with both the membrane and cytosolic fractions of the cell, and the membrane-bound

180 s in Mrp2-null mice were observed in DNA and cytosolic fractions of the kidneys.

181 in chelatable iron in mitochondrial but not cytosolic fractions of the rat hippocampus.

182 e real-time dynamics of the cell-surface and cytosolic fractions of the transporter in living cells.

183 purified by RNA affinity chromatography from cytosolic fractions of TNF-alpha-stimulated human umbili

184 proteins derived from both the membrane and cytosolic fractions of tumor cell lysates.

185 ccharomyces cerevisiae, as well as the free (cytosolic) fractions of each ABP.

186 1 beta were induced by the membrane, but not cytosolic, fraction of M. tuberculosis.

187 hate (GTP)-binding proteins from membrane to cytosolic fractions on Western blots, suggesting that lo

188 In cytosolic fractions, PDE3A-118 and PDE3A-94 comprise >50

189 Endoproteinase LysC digestion of the cytosolic fraction produced a global proteomic sample, c

190 ocalized to detergent-sensitive membrane and cytosolic fractions, respectively.

191 tern blot analysis of the microsomal and the cytosolic fractions revealed that the induced expression

192 s containing nuclei or purified DNA, but not cytosolic fractions, stimulated signaling through TREM2.

193 DR1 protein is not entirely localized in the cytosolic fraction, suggesting that it may interact with

194 the mutants are primarily distributed in the cytosolic fraction, suggesting that these mutations disr

195 pitates from nuclear fractions (but not from cytosolic fractions) the upstream binding factor, which

196 Within the cytosolic fraction, the enzymes of glycolysis, fatty aci

197 inase II were 81 and 19%, whereas within the cytosolic fraction, the relative contributions for hexok

198 ppearance of high-molecular-weight TDP-43 in cytosolic fractions, these findings demonstrate the misl

199 TRADD, FADD, the caspase-8, and TRAF2 in the cytosolic fraction, TNFR1 in the mitochondria was associ

200 ive PAP-1 activity was translocated from the cytosolic fraction to the membrane fraction within 2 min

201 redistribution of TIP47 and adipophilin from cytosolic fractions to the lipid droplet fraction.

202 ignificant portion of TIP47 shifted from the cytosolic fractions to the lipid droplet fractions.

203 NEDD4-1 was identified from Jurkat cytosolic fractions using an enzyme-dead Mdm2 mutant pro

204 rosocysteine, the rate of degradation in the cytosolic fraction was 4.1 +/- 0.3 nmol.min-1.(mg protei

205 However, as expected, p27Kip1 in the cytosolic fraction was greatly reduced following serum s

206 ccordingly, a decrease of PKC content in the cytosolic fraction was observed.

207 ual transcripts in the 'membrane-bound' and 'cytosolic' fractions was quantitated for thousands of ge

208 d Akt and translocation of Galphaq/11 to the cytosolic fraction were maintained in Tg-Y319F hearts.

209 Crude liver homogenates and their cytosolic fractions were assayed for their chymotrypsin-

210 increased oligonucleosomes in the epidermal cytosolic fractions were detected in the diseased mice.

211 informed consent), and cellular nuclear and cytosolic fractions were generated by subcellular fracti

212 1-/- cells, as levels of FGF2 in nuclear and cytosolic fractions were not significantly different.

213 testinal FOXs in which separate membrane and cytosolic fractions were prepared and FOX activity was m

214 oblot analysis, after lysis, the nuclear and cytosolic fractions were separated and analyzed by SDS-P

215 tion with membrane fractions, rather than in cytosolic fractions, where most heterologously expressed

216 d an interaction between 5LO and COX2 in the cytosolic fraction, whereas when H-89 was added, 5LO int

217 Furthermore, pretreatment of the cytosolic fraction with anti-PP2A serum attenuated the g

218 cantly increased in both the nuclear and the cytosolic fractions with denervation.