ライフサイエンスコーパス: deacylate

1 tions can occur only when the P-site tRNA is deacylated.

2 post-transfer editing a misacylated tRNA is deacylated.

3 PLAP was acylated and then progressively was deacylated.

4 bated with increasing concentrations of NaOH-deacylated 1291 LOS.

5 ed [3H]LPS, animals expressing the transgene deacylated a larger fraction of the [3H]LPS taken up by

6 as an independent protein, it was capable of deacylating a mischarged Ala-microhelixPro variant.

7 action known as pretransfer editing, and (3) deacylating a mischarged Ala-tRNA(Pro) variant via a pos

8 This enzyme also is capable of rapidly deacylating a mischarged Ala-tRNA(Pro) variant.

9 Providing recombinant AOAH restored LPS deacylating ability to Aoah(-/-) mice and prevented LPS-

10 aining purified LPS-binding protein, the LPS-deacylating activity of MNC greatly exceeded that of PMN

11 combinantly-expressed and purified MCP1 also deacylates Ala-tRNAs despite lacking known tRNA-editing

12 o the prokaryotic INS domain, was capable of deacylating Ala-tRNAPro and Ala-microhelixPro variants b

13 e ProRS editing domain, is capable of weakly deacylating Ala-tRNAPro.

14 n GC and lactosylceramide (LacCer) and their deacylated analogues.

15 ng the association and dissociation rates of deacylated and aminoacylated tRNAs to the A-site and P-s

16 that acylate the enzyme but are only slowly deacylated and can therefore act also as potent inhibito

17 Deacylated lipid A, deacylated and palmitoylated lipid A, palmitoylated lipi

18 treatment of a ribosomal complex containing deacylated and peptidyl-tRNAs bound in the A/P and P/E s

19 AOAH) is an eukaryotic lipase that partially deacylates and detoxifies Gram-negative bacterial lipopo

20 ressed by antigen (Ag)-presenting cells that deacylates and thereby inactivates LPS in host tissues.

21 oxysuccinimidyl carbamate, ester lipids were deacylated, and the reaction mixtures were subjected to

22 formed by HPLC-PDA-ESI-MS(n) and extended to deacylated anthocyanins and aglycones, obtained, respect

23 The 95-kDa protein also deacylated arachidonoyl groups from 1-O-hexadecyl-2-arac

24 y SDS-polyacrylamide gel electrophoresis and deacylated arachidonoyl-lysophosphatidylcholine (ara-lys

25 and 5-methylcytidine is rapidly degraded and deacylated at 37 degrees C in a trm8-Delta trm4-Delta st

26 bstituted lysoPC, since palmitoyl-lysoPC was deacylated at a much lower rate (7 micromol/(min mg)).

27 by acyloxyacyl hydrolase, a host enzyme that deacylates bacterial lipopolysaccharides.

28 eta-aryl ethers do not, however, produce the deacylated beta-aryl ether intermediates, as was previou

29 Importantly, ABHD17a deacylates BK channels in a site-specific manner because

30 s-His-Cys S-acyltransferase 19 (ZDHHC19) and deacylated by acyl protein thioesterase 1 (APT1).

31 RNALys, and Met-tRNALys were essentially not deacylated by LysRS, indicating that the enzyme does not

32 tructurally elucidated, and then shown to be deacylated by recombinant Tem25.

33 ls that controls membrane trafficking and is deacylated by the acyl protein thioesterase Lypla1.

34 er, carbapenemases such as KPC-2 efficiently deacylate carbapenem acyl-enzymes.

35 Ceramidases deacylate ceramides, important intermediates in the meta

36 ing just one fatty acid, we show that ABHD18 deacylates CL further.

37 with (13)C isotopes, and the accumulation of deacylated CLs, such as MLCL and dilyso-CL, in tafazzin

38 CmaE will only recognize deacylated CmaA for initial complexation.

39 Under identical settings, deacylated cyanin, ferulic and sinapic acids had kinh of

40 In contrast, the INS domain is unable to deacylate Cys-tRNA(Pro), which is hydrolyzed exclusively

41 appear to possess tRNA specificity, readily deacylating Cys-tRNACysin vitro.

42 Sulfonolipid and its deacylated derivative, capnine, are sulfur analogs of ce

43 Lysophospholipids are deacylated derivatives of their bilayer forming phosphol

44 A or DNA containing dG-C8-AAF or the related deacylated dG-C8-AF adduct.

45 Unlike deacylated elongator tRNAs, N-acetyl-aminoacyl-tRNAs and

46 In this study, we demonstrate that ceramide-deacylating enzyme acid ceramidase (AC) was preferential

47 OS generated by treatment of wt LOS with the deacylating enzyme, acyloxyacylhydrolase.

48 s that lacked acyloxyacyl hydrolase, the LPS-deacylating enzyme, prolonged drainage of fully acylated

49 The substrate of PvdP, deacylated ferribactin, is secreted by a DeltapvdM mutan

50 the C7 hydroxyl of baccatin III must remain deacylated for enzyme function.

51 on is accelerated when the protein is in its deacylated form (dSP-C).

52 y of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (P

53 However, the production of its deacylated form globotriaosylsphingosine (lyso-Gb3) is a

54 ophosphocholine and is specific for the sn-2-deacylated form of plasmalogen.

55 ines is caused by a rapid degradation of the deacylated form of the abnormal mt-tRNA(Val).

56 the conversion of acylated peptides to their deacylated forms.

57 zed an enzyme acyl-protein thioesterase that deacylates Galpha proteins and at least some other thioa

58 mechanical and physicochemical properties of deacylated gellan matrices is presented.

59 f acid ceramidase, the lysosomal enzyme that deacylates GluCer to GluSph, prevented mTOR hyperactivit

60 iesterase (GDPD) catalyzes the hydrolysis of deacylated glycerophospholipids to glycerol phosphate an

61 They also fail to deacylate Gpa1p, the yeast G alpha subunit, in metabolic

62 a trapped complex of SIRT6 in the process of deacylating H3K27, demonstrating how SIRT6 undergoes con

63 M1 protein, while the stunting of fusion by deacylated HA acting in isolation may be balanced by oth

64 Compared to wild-type HA, deacylated HA is correlated with released particles with

65 ed cardiolipin progressing to the completely deacylated headgroup.

66 the p-hydroxybenzoate is cleaved before the deacylated lignin is depolymerized.

67 The role of 3-O-deacylated lipid A among nitrogen-fixing endosymbionts,

68 in production of significant amounts of 3'-O-deacylated lipid A in growing cultures.

69 bility to add aminoarabinose to lipid A, 3-O-deacylated lipid A species were not detected, despite th

70 Finally, deacylated lipid A species were not observed in some cli

71 lso contains a significant proportion of 3-O-deacylated lipid A species.

72 Deacylated lipid A, deacylated and palmitoylated lipid A

73 y, we demonstrated that the mutant lacks 3-O-deacylated lipid A.

74 Further analysis of internally radiolabeled deacylated lipids from the SmT morphotype, by high-perfo

75 erize the structure and branching pattern of deacylated LOS of E. coli.

76 Here we report the characterization of deacylated LOS of LPS by activated-electron photodetachm

77 Mass spectrometry analysis of the O-deacylated LOS of the R127K point mutation confirmed the

78 Analysis of HF-treated and O-deacylated LOS revealed three major components present i

79 AH treatment of LOS-sCD14 produced partially deacylated LOS still complexed with sCD14.

80 When the O-deacylated LOS were analyzed by mass spectrometry, both

81 AOAH-deficient mice did not deacylate LPS and, whereas their inflammatory responses

82 the cells' ability to kill Escherichia coli, deacylate LPS, and degrade bacterial protein.

83 H can then be used by the recipient cells to deacylate LPS.

84 AOAH and secrete it into urine, where it can deacylate LPS.

85 n this current study, we have analyzed the O-deacylated LPS and free oligosaccharides from three tran

86 Enzymatically deacylated LPS is much less potent than LPS at inducing

87 Transgenic mice deacylated LPS more rapidly than did wild-type controls.

88 ponsive; and 2) substoichiometric amounts of deacylated LPS that block LPS signaling at a site distal

89 The immunomodulatory properties of PagL-deacylated LPS were compared with another pentaacyl form

90 h only one acyl chain (LTA-1) and completely deacylated LTA (LTA-0).

91 ulation of glucosylceramide (GluCer) and its deacylated metabolite glucosylsphingosine (GluSph).

92 -tRNA synthetase is unable to hydrolytically deacylate misacylated tRNA(Val) terminating in 3'-pyrimi

93 (Val) terminating in 3'-pyrimidines but does deacylate mischarged tRNA(Val) terminating in adenosine

94 ited earlier studies demonstrated that AlaXp deacylated mischarged tRNA(Ala) in vitro, the significan

95 Ss possess an amino acid editing domain that deacylates mischarged Ala-tRNAPro, editing of Cys-tRNAPr

96 iposomes with either a low or high dose of 3-deacylated monophosphoryl lipid A (MPL) and administered

97 A vaccine adjuvanted with deacylated monophosphoryl lipid A (MPLA) failed to prote

98 glycoprotein D from HSV-2 with alum and 3-O-deacylated monophosphoryl lipid A as an adjuvant; contro

99 oprotein-D-subunit vaccine with alum and 3-O-deacylated-monophosphoryl lipid A in subjects whose regu

100 t charged with the starved amino acid became deacylated more rapidly than tRNAs charged with the star

101 ectrospray ionization mass spectrometry of O-deacylated MS11mkC LOS produced ions consistent with kno

102 ylation interrupted HA-M1 interactions since deacylated mutant HA failed to incorporate an M1 layer w

103 lso exhibits an acylase activity, capable of deacylating N-acetyl-Met-Leu-p-nitroanilide and N-triflu

104 In contrast, ValRS can deacylate non-cognate amino acids from the 2'-OH.

105 RRF flexibility plays a role in removing the deacylated P-site tRNA during termination of translation

106 The deacylated P-site tRNA has moved into a partly transloca

107 EF-G, pre-translocation ribosomes containing deacylated P-site tRNA undergo spontaneous intersubunit

108 ents increases the synthesis of enzymes that deacylate, palmitoylate, hydroxylate, and attach aminoar

109 and 6-fold above those of the commercial and deacylated PEI25s, respectively; moreover, PEI87 deliver

110 ation of the potential of such linear, fully deacylated PEIs in gene therapy for lung diseases, syste

111 In other bacteria, GlpQ hydrolyzes deacylated phospholipids to glycerol-3-phosphate (G3P) w

112 G3P exist among borreliae: (i) hydrolysis of deacylated phospholipids, (ii) reduction of DHAP, and (i

113 vestigated purified and, more importantly, O-deacylated pnLTA, which is most suitable for NMR spectro

114 The deacylated products of the soluble derivatives of phosph

115 ,4-P2, as determined by HPLC analysis of the deacylated products.

116 ubiquitously expressed cellular enzyme that deacylates protein lysine residues using NAD(+) as a cof

117 ther, these abnormalities suggest that SIRT5 deacylates protein substrates involved in cellular oxida

118 ted with 1 M hydroxylamine which is known to deacylate proteins.

119 oxyacyl hydrolase, the leukocyte enzyme that deacylates purified LPS, to attack LPS residing in the b

120 APT1) was identified as an enzyme capable of deacylating some thioacylated proteins in vitro.

121 Deacylated SP-C (dSP-C), unchanged in composition and se

122 ic G proteins cycle between thioacylated and deacylated states in a receptor-regulated fashion.

123 However, the features that allow KPC-2 to deacylate substrates more rapidly than non-carbapenemase

124 The colibactin peptidase ClbP deacylates synthetic precolibactin 886 to form a non-gen

125 K(m) values of 0.42 mM and 0.40 mM for the N-deacylated taxoid and benzoyl-CoA, respectively.

126 ntermediate and subsequently employ water to deacylate the beta-lactam and release product.

127 rty of dendritic cells (DCs), the ability to deacylate the lipid A moiety of gram-negative bacterial

128 NA synthetases have an editing activity that deacylates the mischarged amino acid before capture by t

129 eta-hydrolase domain-containing protein 17a) deacylates the stress-regulated exon domain of large con

130 Phospholipids are extracted and deacylated to glycero-head groups, which are then separa

131 diolipin (CL) that is synthesized de novo is deacylated to monolysocardiolipin (MLCL), which is reacy

132 almitoylglycerophosphocholine, which is then deacylated to provide substrates for chain elongation an

133 During protein synthesis, deacylated transfer RNAs leave the ribosome via an exit

134 therefore be recycled by releasing mRNA and deacylated tRNA and by dissociating ribosomes into subun

135 igatin and MCT-1/DENR can promote release of deacylated tRNA and mRNA from recycled 40S subunits afte

136 the termination step of protein synthesis, a deacylated tRNA and mRNA remain associated with the ribo

137 e post-peptidyl transferase translocation of deacylated tRNA and peptidyl tRNA to the ribosomal E- an

138 at the rate-limiting step is dissociation of deacylated tRNA and/or amino acid product and highlights

139 tRNA binds in the classical P site, whereas deacylated tRNA binds mostly in an intermediate P/E posi

140 While evidence suggests that deacylated tRNA binds the HisRS domain for kinase activa

141 k(on)) of tRNA dramatically, suggesting that deacylated tRNA binds the P site of the ribosome via the

142 acilitates binding, movement, and release of deacylated tRNA by remodeling the structure of the 50S s

143 le movements coupled to the translocation of deacylated tRNA during protein synthesis.

144 unit E site that interacts with the elbow of deacylated tRNA during protein synthesis.

145 s been proposed to facilitate the removal of deacylated tRNA from the E-site.

146 ed by two tRNA molecules and that release of deacylated tRNA from the exit (E) site is uncoupled from

147 binding of EF-G would trigger the removal of deacylated tRNA from the P site by moving RRF toward the

148 and is translocated to the P-site, releasing deacylated tRNA from the P- and E-sites.

149 ribosomal subunits, and release of mRNA and deacylated tRNA from the PoTC, are unclear.

150 , a function performed by eEF1A, by removing deacylated tRNA from the ribosomal Exit site.

151 ation ribosomes containing peptidyl-tRNA and deacylated tRNA in the classical P/P and E/E states, res

152 te constants reveals that destabilization of deacylated tRNA in the E-site, rearrangement of peptidyl

153 The deacylated tRNA in the peptidyl (P) site moves into a pr

154 Movement of the acceptor stem of deacylated tRNA into the 50S E site and EF-G binding to

155 state of binding that precedes the entry of deacylated tRNA into the F (final) site from which it di

156 between the ribosomal L1 stalk and the newly deacylated tRNA is established spontaneously upon peptid

157 is at initiation in response to increases in deacylated tRNA levels in the cell.

158 Deacylated tRNA may be a factor negatively regulating p7

159 ter two cycles of elongation, when the first deacylated tRNA reached the E-site after translocation f

160 r translational termination, mRNA and P site deacylated tRNA remain associated with ribosomes in post

161 specificity to EF-G2(mt), and show that the deacylated tRNA remains with the dissociated 39S subunit

162 regulatory role of the exit (E) site, where deacylated tRNA spontaneously dissociates from the trans

163 We show that the 3'-end of the deacylated tRNA that is formed after transpeptidation do

164 This reduction in deacylated tRNA was accompanied by decreased synthesis o

165 ad domain and contact of the acceptor arm of deacylated tRNA with helix 68 of 23S rRNA.

166 the absence of editing lowered the amount of deacylated tRNA(Phe) in the cell.

167 ibosomes remain associated with mRNA, P-site deacylated tRNA, and release factor eRF1 and must be rec

168 PoTC), composed of the ribosome, mRNA, and a deacylated tRNA, is processed by the concerted action of

169 h is activated by amino acid deprivation via deacylated tRNA, was found to be active in rodent brain,

170 Gcn2p is activated by deacylated tRNA, which accumulates when tRNA aminoacylat

171 y the eIF2alpha kinase, GCN2, in an apparent deacylated tRNA-independent fashion.

172 tivity of elongation factor-2 and removal of deacylated tRNA.

173 lipid-independent trans editing factor that deacylates tRNA.

174 usly unidentified hybrid state in which only deacylated-tRNA adopts its hybrid (P/E) configuration.

175 c ATPase proposed to catalyze the release of deacylated-tRNA from the ribosomal E-site.

176 the RelA/SpoT homolog (Rsh) upon entry of a deacylated-tRNA in a translating ribosome.

177 Aminoacyl-tRNA, peptidyl-tRNA, and deacylated-tRNA were bound in various combinations to th

178 The position of deacylated tRNAfMet depends on the buffer condition used

179 Either deacylated tRNAfMet or fMet-tRNAfMet were bound to the 7

180 ed accumulation of Tyr-tRNAPhe (5%), but not deacylated tRNAPhe during amino acid starvation, limitin

181 each crucial for Gcn2 function-supports that deacylated tRNAs activate Gcn2 and exemplifies how a met

182 When mRNA and deacylated tRNAs are bound to the 70S ribosome and EF-G-

183 nthetase (HisRS) postulated to bind multiple deacylated tRNAs as a general sensor of starvation.

184 inhibit protein synthesis by competing with deacylated tRNAs for E site binding.

185 s region is involved in the translocation of deacylated tRNAs from the P to the E site.

186 We propose that accumulation of deacylated tRNAs in amino acid-starved cells can functio

187 onditions favoring movement of the resulting deacylated tRNAs into the P/E hybrid state, leads to sim

188 Pairing of amino acids and deacylated tRNAs is catalyzed by aminoacyl-tRNA syntheta

189 cordance with this model, GCN2 bound several deacylated tRNAs with similar affinities, and aminoacyla

190 se (IleRS) and valyl-tRNA synthetase (ValRS) deacylate Val-tRNA(Ile) and Thr-tRNA(Val), respectively.

191 lving the carbapenem C-3 carboxylate and the deacylating water and stabilization by protonated N-4, a

192 s Asn170 may prevent the inactivation of the deacylating water by the 6a-hydroxyethyl substituent of

193 s Asn170 may prevent the inactivation of the deacylating water by the 6alpha-hydroxyethyl substituent

194 s A beta-lactamases, it is proposed that the deacylating water molecule is deactivated by interaction

195 Specifically, Asn170 that coordinates the deacylating water molecule is misaligned, in both the fr

196 so as to block the approach of a potentially deacylating water molecule.

197 roup interacts with Asn132, but not with the deacylating water molecule.

198 ation and prevent their interaction with the deacylating water molecule.

199 e binding groove) to the active site and the deacylating water.

200 K84, with implications for activation of the deacylating water.

201 24S) is a relatively unreactive acyl enzyme, deacylating with a pKa of 7.1 and a rate constant of 0.0

202 rs the salvage/recycling pathway and becomes deacylated, yielding sphingosine, re-acylation of which