ライフサイエンスコーパス: deadenylation

1 ich is initiated by poly(A) tail shortening (deadenylation).

2 stimulates removal of its poly(A) tail (i.e. deadenylation).

3 g evidence indicates that TOBs regulate mRNA deadenylation.

4 contains multiple enzymes that catalyze mRNA deadenylation.

5 nanos1 and TDRD7 from miR-430b-mediated RNA deadenylation.

6 3 acts through a general mechanism to affect deadenylation.

7 dependent and might protect the targets from deadenylation.

8 F1 also represses translation independent of deadenylation.

9 is sufficient for repression, independent of deadenylation.

10 nd to be required for eIF4E effects on COX17 deadenylation.

11 tide addition to protect messenger RNAs from deadenylation.

12 domain of PAB1 blocked both their effects on deadenylation.

13 caf1b, AtCAF1b targets AtPI4Kgamma3 mRNA for deadenylation.

14 bunits CAF1 and CCR4 as factors required for deadenylation.

15 capping/5'-to-3' or 3'-to-5' direction after deadenylation.

16 RNAs, premature termination codons, and mRNA deadenylation.

17 larization, correspondingly accelerated CCR4 deadenylation.

18 ors involved in translation are required for deadenylation.

19 As that have previously undergone more rapid deadenylation.

20 PAB1 oligomerization is a required step for deadenylation.

21 binding protein (PAB1) as necessary for CCR4 deadenylation.

22 4p and Pop2p deadenylases in Mpt5p-regulated deadenylation.

23 ntially regulate transcripts at the level of deadenylation.

24 zyme to the target mRNA, thereby stimulating deadenylation.

25 d mRNA decay initiated by miRNA-guided rapid deadenylation.

26 anced mt-mRNA decay by translation-dependent deadenylation.

27 residue predicted to bind magnesium disrupts deadenylation.

28 ynthetic RNA substrate in a process known as deadenylation.

29 transcripts, apparently by initiating their deadenylation.

30 vo for controlling the rate of specific mRNA deadenylation.

31 n (PABP) in facilitating both protection and deadenylation.

32 pathway for closed-loop forming mRNAs after deadenylation.

33 thways for translational repression and mRNA deadenylation.

34 rotects RNA(3'-rA) from ligation and further deadenylation.

35 3'-termini, consistent with protection from deadenylation.

36 from accumulating despite accelerated target deadenylation.

37 ciated factor 1 (CAF1), is a major player in deadenylation.

38 xpression stabilized TNF mRNA by suppressing deadenylation.

39 tibility to mutations inhibiting cytoplasmic deadenylation, a process required for both 5'-to-3' and

40 trolled by a member of the Pumilio family of deadenylation activators Puf3p, requires an active Ccr4p

41 quitination of CNOT7 by MEX-3C regulates its deadenylation activity and is required for MHC-I mRNA de

42 4A2 into the CCR4-NOT complex inhibits CNOT7 deadenylation activity in contrast to DDX6 which enhance

43 This 3'-deadenylation activity is metal-dependent and requires a

44 n extensively characterized exonuclease with deadenylation activity that controls mRNA stability in p

45 crystal structures are essential for the 3'-deadenylation activity, suggesting that 3'-adenosine may

46 evaluation of patient cells revealed reduced deadenylation activity.

47 Deadenylation, also called poly(A) tail shortening, is t

48 Deadenylation, also called poly(A) tail shortening, is t

49 cr4-Not complex initiates mRNA decay through deadenylation and activation of decapping.

50 niaD transcript are effected at the level of deadenylation and are dependent on its 3' UTR.

51 results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during ear

52 mRNA is sufficient for both protection from deadenylation and deadenylation-independent decapping an

53 ch suggests a possible link between the mRNA deadenylation and decapping machinery.

54 en established as a target of Puf3p-mediated deadenylation and decapping.

55 The ENE inhibits deadenylation and decay in nuclear extract and prevents

56 scription-pulsing approaches to monitor mRNA deadenylation and decay kinetics, we demonstrate the exi

57 rameric DST element from soybean accelerated deadenylation and decay of a reporter transcript.

58 Drosophila Tis11 promoted both deadenylation and decay of a target transcript in this h

59 the beta-globin reporter mRNA promoted rapid deadenylation and decay of hypo-adenylated reporter mRNA

60 TTP is known to stimulate the deadenylation and decay of mRNAs possessing one or more

61 Accelerated deadenylation and decay of the transcript follows riboso

62 We observed that during the biphasic deadenylation and decay process of this mRNA, dTIS11 enh

63 ich elements in mRNAs and promote transcript deadenylation and decay.

64 nitiation of target mRNAs, followed by their deadenylation and decay.

65 ional repression and/or messenger RNA (mRNA) deadenylation and decay.

66 slational inhibition, followed by effects on deadenylation and decay.

67 m whereby TTP targets inflammatory mRNAs for deadenylation and decay.

68 elements in target mRNAs, and promote their deadenylation and decay.

69 ch regions in target mRNAs, leading to their deadenylation and decay.

70 sed binding to RNA as well as inhibited mRNA deadenylation and decay.

71 They target mRNAs for rapid deadenylation and degradation and may enhance decapping.

72 Thus, BTG1/2 promote the deadenylation and degradation of mRNA to secure T cell q

73 d alterations in activities that govern both deadenylation and degradation of the mRNA body.

74 as the mRNA encoding TNF, and promotes their deadenylation and degradation.

75 ession and/or promoting messenger RNA (mRNA) deadenylation and degradation.

76 many maternal proteins are downregulated by deadenylation and destabilization of their encoding mRNA

77 taining AU-rich elements (ARE), resulting in deadenylation and destabilization of these transcripts.

78 elements within certain mRNAs, resulting in deadenylation and destabilization of those mRNAs.

79 in certain cellular mRNAs, leading to their deadenylation and destabilization.

80 h elements (AREs) in mRNAs, leading to their deadenylation and destabilization.

81 s, leading to increases in the rates of mRNA deadenylation and destruction.

82 rveillance complex can target mRNAs to rapid deadenylation and exosome-mediated degradation.

83 s reducing the ability of the TTP to promote deadenylation and instability of the mRNA.

84 the major enzyme complex that catalyzes mRNA deadenylation and is conserved among eukaryotes.

85 ping and distinct roles with respect to mRNA deadenylation and mediation of stress responses.

86 with a functional assay directly monitoring deadenylation and mRNA decay to characterize the effects

87 a Caf1 catalytically inactive mutant impairs deadenylation and mRNA decay.

88 rent levels by regulating pre-mRNA splicing, deadenylation and mRNA stability.

89 upregulated after infection but degraded by deadenylation and progressive 3'-to-5' degradation.

90 While required for target mRNA deadenylation and silencing, this site is not sufficient

91 decapping-independent NMD pathway involving deadenylation and subsequent 3'-->5' exonucleolytic deca

92 stabilizes host stress response mRNAs after deadenylation and subsequent cleavage near the adenylate

93 rated to direct target RNA reduction through deadenylation and subsequent degradation of target trans

94 translated region of mRNAs, leading to their deadenylation and subsequent degradation.

95 Exit from M phase seems to require deadenylation and subsequent translational silencing of

96 Furthermore, transcript deadenylation and the consequent dissociation of poly(A)

97 that the interplay between polyadenylation, deadenylation and tumour-suppressor factors might preven

98 t encoding TNF, and increases their rates of deadenylation and turnover.

99 m zinc finger (TZF) domain, TTP promotes the deadenylation and ultimate decay of target transcripts.

100 ed regions (UTRs) and subsequently promoting deadenylation and ultimate destruction of those mRNAs.

101 unique properties and is both protected from deadenylation and undergoes deadenylation-independent de

102 Because translation, deadenylation, and decay are closely linked processes, i

103 nslational repression occurs before complete deadenylation, and disrupting deadenylation with use of

104 untranslated region and recruits decapping, deadenylation, and exonucleolytic enzymes to PBs for RNA

105 ranslational repression precedes target mRNA deadenylation, and identify GW182, PABP, and deadenylase

106 mRNAs that affects translation termination, deadenylation, and mRNA decay.

107 egulator, orchestrating gene expression, RNA deadenylation, and protein ubiquitination.

108 Aly appears to protect the poly(A) tail from deadenylation, and REF/Aly-stabilized transcripts are fu

109 We also find that TOBs' actions in deadenylation are independent of the phosphorylation sta

110 The model results reveal the rhythmicity in deadenylation as the strongest contributor to the rhythm

111 role for ubiquitin in regulating MHC-I mRNA deadenylation as ubiquitination of CNOT7 by MEX-3C regul

112 Deadenylation assays confirm the functional importance o

113 tion assays and in cell-free RNA binding and deadenylation assays, suggesting that it may play roles

114 P domain) of PAB1 substantially reduces CCR4 deadenylation at non-PUF3-controlled mRNA and correspond

115 ression through translational repression and deadenylation but not cleavage.

116 PABPs accelerate miRNA-mediated deadenylation, but this contribution can be modulated by

117 rporation of guanosine into poly(A)-inhibits deadenylation by both Pan2 and Caf1.

118 The regulation of deadenylation by p38 MAPK was found to be specific becau

119 nhibition of 3' cleavage, strongly activates deadenylation by PARN in the presence of CstF-50, and th

120 rting a PER-dependent inhibition of tim mRNA deadenylation by POP2.

121 ng its CNBD could still activate target mRNA deadenylation by purified recombinant Schizosaccharomyce

122 ent model was proposed in which TOBs promote deadenylation by recruiting CAF1-CCR4 deadenylase comple

123 so implicated in mediating the repression of deadenylation by the 3' UTR of another alphavirus, Venez

124 trate polyA RNA, facilitating efficient mRNA deadenylation by the intact Pan2-Pan3 complex.

125 hat reduced translation, while not affecting deadenylation by themselves or when combined with ccr4De

126 -adenylated intermediates are substrates for deadenylation by yeast 5'Deadenylase.

127 mRNA was stabilized in mutants defective in deadenylation (ccr4Delta), mRNA decapping (dcp1), and th

128 CCR4-CAF1-NOT complex is a major cytoplasmic deadenylation complex in yeast and mammals.

129 The conserved eukaryotic Pan2-Pan3 deadenylation complex shortens cytoplasmic mRNA 3' polyA

130 deadenylase, a key component of the CCR4-NOT deadenylation complex, alters behavioral rhythms.

131 tin ligases, associates with the cytoplasmic deadenylation complexes and ubiquitinates CNOT7(Caf1), t

132 the predominant effect of microRNA-mediated deadenylation concurrently shifts from translational rep

133 em zinc finger domain; it then promotes mRNA deadenylation, considered to be the rate-limiting step i

134 that inhibit translation and stimulate mRNA deadenylation, decapping, and decay.

135 of mRNA decay enzymes, and help explain how deadenylation, decapping, and exonucleolytic decay can a

136 ough a large variety of mechanisms including deadenylation, decapping, and P-body targeting.

137 AREs have been shown to directly activate deadenylation, decapping, or 3'-to-5' exonucleolytic dec

138 anding/assembly" platform for formation of a deadenylation/decay mRNA-protein complex on an mCRD-cont

139 This deadenylation deficiency caused an early DNA damage resp

140 A degradation and repress expression by both deadenylation-dependent and -independent mechanisms, usi

141 Thus, human PUMs are repressors capable of deadenylation-dependent and -independent modes of repres

142 The ENE protects PAN RNA from a rapid deadenylation-dependent decay pathway via formation of a

143 h1 specifically affects mRNA turnover in the deadenylation-dependent decay pathway, but does not act

144 s the 5' cap-structure of mRNA and initiates deadenylation-dependent decay.

145 ound that while Olfr mRNAs are degraded by a deadenylation-dependent mechanism, they are largely prot

146 een studied extensively and is degraded by a deadenylation-dependent mechanism.

147 nd other components of the Lsm1p-Lsm7p/Pat1p deadenylation-dependent mRNA decapping complex were also

148 ody-associated 5' to 3' mRNA decay pathways, deadenylation-dependent mRNA decay (DDD) and nonsense-me

149 Therefore, our data uncover a new, deadenylation-dependent mtRNA maturation pathway in huma

150 e virus has evolved a way to avoid the major deadenylation-dependent pathway of mRNA decay.

151 res a 5' cap structure on the mRNA; however, deadenylation does not.

152 TOE1 displays a functional deadenylation domain and has been shown to participate i

153 teraction with PABPC1 is necessary for TOB's deadenylation-enhancing effect.

154 eviously characterized as a Mn(2+)-dependent deadenylation exoribonuclease.

155 pathways of eukaryotic mRNA decay occur via deadenylation followed by 3' to 5' degradation or decapp

156 yotic mRNA decay proceeds through an initial deadenylation followed by 5' end decapping and exonucleo

157 f eukaryotic mRNA degradation initiates with deadenylation followed by decapping and 5' to 3' degrada

158 t, a major pathway of mRNA decay begins with deadenylation followed by decapping and 5'-3' exonucleas

159 major pathway for mRNA decay is initiated by deadenylation followed by decapping and 5'-3' exonucleol

160 sequence-specific manner, resulting in mRNA deadenylation followed by exonucleolytic decay, mRNA end

161 e generalized process of mRNA decay involves deadenylation followed by release from translating polys

162 ic messenger RNA (mRNA) turnover begins with deadenylation, followed by decapping and 5' to 3' exonuc

163 athway of mRNA decay in yeast initiates with deadenylation, followed by mRNA decapping and 5'-3' exon

164 ivity, this activity is not required for its deadenylation function in vivo, and CCR4 is the primary

165 Since deadenylation has been reported to be required for P bod

166 are important enzymes for catalysis of mRNA deadenylation in eukaryotes.

167 This demonstrated accelerated deadenylation in KO cells on PATs < 75 nucleotides and p

168 ings highlight the critical role of rhythmic deadenylation in regulating poly(A) rhythms and circadia

169 that OsCAF1 proteins may be involved in the deadenylation in rice.

170 Stabilization occurs as a result of slower deadenylation in the ssa1(ts) strain, suggesting that Hs

171 The rate of deadenylation in vitro by yCCR4 and mCAF1 were both stro

172 ) interact with the polyA tail, (ii) inhibit deadenylation in vitro, and (iii) stabilize transcripts

173 he CCF-1/Pop2p deadenylase and can stimulate deadenylation in vitro, and that CCF-1 is partially resp

174 reases stability in vivo but does not affect deadenylation in vitro, comparable to the effects of del

175 nuclease involved in the mCRD-mediated rapid deadenylation in vivo and also associated with UNR.

176 s support the model that the control of CCR4 deadenylation in vivo occurs in part through the removal

177 AF1-specific functional regions required for deadenylation in vivo, we targeted for mutagenesis six r

178 ferative transcription factor, enhances mRNA deadenylation in vivo.

179 roteins are required for full repression and deadenylation in vivo; their removal dramatically stabil

180 A second, deadenylation independent mechanism was revealed by the

181 t for both protection from deadenylation and deadenylation-independent decapping and an extended poly

182 cp2p to the complex appears to ensure rapid, deadenylation-independent decapping of the mRNA.

183 pts are efficiently recognized, targeted for deadenylation-independent decapping, and show NMD trigge

184 tion from either endonucleolytic cleavage or deadenylation-independent decapping.

185 h protected from deadenylation and undergoes deadenylation-independent decapping.

186 ion codons (nonsense mRNAs) are targeted for deadenylation-independent degradation in a mechanism tha

187 BP delta mRNA degradation, which suggested a deadenylation-independent pathway.

188 The deadenylation-independent repression requires a 5' cap s

189 mRNA deadenylation is a key process in the regulation of tran

190 P-bodies are not detected when deadenylation is blocked and are restored when the block

191 When deadenylation is impaired, P-body formation is not resto

192 Transcript is stabilized when accelerated deadenylation is impeded by blocking translation initiat

193 In Saccharomyces cerevisiae, deadenylation is primarily carried out by the Ccr4p and

194 We demonstrate that deadenylation is required for mammalian P-body formation

195 Deadenylation is the first and rate-limiting step in the

196 One consequence of deadenylation is the formation of nontranslatable messen

197 Deadenylation is the major step triggering mammalian mRN

198 mRNA deadenylation is under the control of cis-acting regulat

199 Poly(A) tail shortening, also termed deadenylation, is the rate-limiting step of mRNA degrada

200 gradation appears to occur primarily through deadenylation-linked mechanisms, with little contributio

201 RNA binding by preventing recruitment of the deadenylation machinery.

202 o sensitize PABP dissociation in response to deadenylation machinery.

203 , the main catalytic subunit of the CCR4-NOT deadenylation machinery.

204 dividual mRNAs presumably by protection from deadenylation (Mattijssen et al., 2017).

205 s suggest that inhibition of splicing and/or deadenylation may be effective therapies for Lsm1-over-e

206 We report a deadenylation mechanism that controls the oscillations o

207 protein-were implicated in the repression of deadenylation mediated by the SINV 3' UTR.

208 We found that degradation occurs via mRNA deadenylation, mediated by the CCR4-NOT complex.

209 ly(A) tail exacerbate dependency on PABP for deadenylation, more potent miRNA-binding sites partially

210 se results support a dynamic interplay among deadenylation, mRNP remodeling, and P-body formation in

211 RNase H treatment indicated that rapid deadenylation occurred concomitant with degradation of t

212 uration of mutated RNA14 was unaffected, but deadenylation occurred rapidly.

213 binds to RNAs containing AREs, and promotes deadenylation of a model ARE transcript in a cell-based

214 We conclude that TTP can promote the deadenylation of ARE-containing, polyadenylated substrat

215 TTP and its related proteins stimulated the deadenylation of ARE-containing, polyadenylated transcri

216 TTP may remain poised to rapidly reactivate deadenylation of bound transcripts to downregulate gene

217 hypothesis where mTOR is the tag, preventing deadenylation of CaMKIIalpha mRNA, whereas HuD captures

218 4p proteins are required for Mpt5p-regulated deadenylation of HO.

219 ne hydrolase (MTAN) catalyzes the hydrolytic deadenylation of its substrates to form adenine and 5-me

220 limits expression of specific genes through deadenylation of mRNA poly(A) tails, enabling positive s

221 to CAF1, plays an essential role in the CCR4 deadenylation of mRNA.

222 nopus oocytes and early embryos and prevents deadenylation of mRNAs, suggesting its importance in the

223 ant role in the translational repression and deadenylation of mRNAs.

224 on and decay in nuclear extract and prevents deadenylation of naked RNA by a purified deadenylase, li

225 p38 MAPK activation inhibited the deadenylation of reporter mRNAs containing either the cy

226 e, we reconstitute accelerated and selective deadenylation of RNAs containing AU-rich elements (AREs)

227 Destabilization and deadenylation of RP transcripts were impaired in an rpb4

228 Regulated polyadenylation and deadenylation of specific mRNAs is involved in oogenesis

229 TTP can direct the deadenylation of substrate mRNAs when tethered to a hete

230 of TTP and its family members to promote the deadenylation of such transcripts in intact cells.

231 cytoplasmic deadenylases and promotes rapid deadenylation of target mRNAs both in vitro and in cells

232 We also show that miR-430 accelerates the deadenylation of target mRNAs.

233 by p38 MAPK was found to be specific because deadenylation of the beta-globin reporter mRNA either la

234 PUF3 was shown to affect PAN2 deadenylation of the COX17 mRNA independent of the prese

235 In addition, the rapid deadenylation of the COX17 mRNA, which is controlled by

236 PUF3 in Saccharomyces cerevisiae accelerates deadenylation of the COX17 mRNA.

237 s, and this has been shown to be mediated by deadenylation of the mRNA and inhibition by the Bruno re

238 ion intermediates revealed that the complete deadenylation of the mRNA triggers its decapping and dec

239 binding of TTP to the RNA and the subsequent deadenylation of the poly(A) tail.

240 o be due to inhibition of polyadenylation or deadenylation of the transcript, followed by exosomal de

241 veals that miRNAs cause not only accelerated deadenylation of their targets but also accelerated deca

242 em CCCH zinc finger protein family, promotes deadenylation of tumor necrosis factor-alpha and granulo

243 responsible for mediating the repression of deadenylation of viral mRNAs.

244 This block in deadenylation of viral transcripts was recapitulated in

245 unction as microRNA (miRNA) mimics in either deadenylation or guided mRNA cleavage (RNAi).

246 To investigate whether it regulates deadenylation or the decay of the mRNA body, we used a t

247 nscripts, thereby causing their degradation, deadenylation, or inhibiting their translation.

248 decay process of this mRNA, dTIS11 enhances deadenylation performed by the CCR4-CAF-NOT complex whil

249 gulation processes, including transcription, deadenylation, polyadenylation, and degradation.

250 The mRNA deadenylation process, catalyzed by the CCR4 deadenylase

251 unclear whether TOBs' antiproliferative and deadenylation-promoting activities are connected.

252 l a link between TOBs' antiproliferative and deadenylation-promoting activities.

253 and correspondingly blocked eIF4E effects on deadenylation, PUF3 essentially bypassed this P domain r

254 denylated mRNAs, enabling the large range in deadenylation rate constants to impart a similarly large

255 lasm, they have a broad (1000-fold) range of deadenylation rate constants, which correspond to cytopl

256 o shape maternal mRNA stability by affecting deadenylation rate in a translation-dependent manner.

257 of the NOT genes can lead to defects in mRNA deadenylation rates.

258 the p38 MAPK pathway predominantly regulates deadenylation, rather than decay of the mRNA body, and t

259 Shortening of the poly(A) tail, termed deadenylation, reduces transcript stability and inhibits

260 ecapping, and show NMD triggered accelerated deadenylation regardless of the position of the nonsense

261 Since deadenylation regulates mRNA decay and/or translational

262 molecular mechanism underlying TOB-promoted deadenylation remains unclear.

263 reverses DNA adenylation but the context for deadenylation repair is unclear.

264 MicroRNAs regulate gene expression through deadenylation, repression, and messenger RNA (mRNA) deca

265 The ability of TTP to promote transcript deadenylation required Mg(2+), but not ATP or prior capp

266 nuclease activity resides in Pan2, efficient deadenylation requires Pan3.

267 hrough its contact to CCR4, has functions in deadenylation separate from its contact to CCR4.

268 essing, indicating that oligoadenylation and deadenylation set rates of hTR maturation.

269 In yeast, the NMD pathway bypasses the deadenylation step and directly targets PTC-containing m

270 The regulation of the critical deadenylation step and its relationship with RNA-process

271 e fate of mammalian mRNA is modulated at the deadenylation step by a protein that recruits poly(A) nu

272 d for and regulates global mRNA decay at the deadenylation step in Saccharomyces cerevisiae.

273 mediated by the mCRD, demonstrating that the deadenylation step is coupled to ongoing translation of

274 ii) the hCaf1z subunit, in addition to rapid deadenylation, subjects substrate RNAs to slow exonucleo

275 thway, a nonsense codon triggers accelerated deadenylation that precedes decay of the PTC-containing

276 onsisting of a cycle of oligoadenylation and deadenylation that regulates the production of mature hu

277 ntify a new role for ubiquitin in regulating deadenylation, the initial and often rate-limiting step

278 y conserved PUF proteins stimulate CCR4 mRNA deadenylation through binding to 3' untranslated region

279 ese results imply that CAF1, while affecting deadenylation through its contact to CCR4, has functions

280 ed for P body formation, viral inhibition of deadenylation, through Pan3 degradation, is a potential

281 quired for binding CCR4, reduced the rate of deadenylation to a lesser extent and resulted in in vivo

282 -bodies, suggesting a role of TOB in linking deadenylation to the P-bodies.

283 he genes in vertebrates, where they regulate deadenylation, translation, and decay of the target mess

284 inhibition of 3' cleavage and activation of deadenylation upon DNA damage.

285 g motifs, with miR-430 and AREs causing mRNA deadenylation upon genome activation.

286 ochondrial protein synthesis showed that the deadenylation was dependent on translation.

287 Transcript deadenylation was not stimulated when a mutant TTP prote

288 Finally, deadenylation was shown to enhance mRNA decay, explainin

289 tion initiation factor known to control CCR4 deadenylation, was shown to affect PAN2 activity in vivo

290 Both eIF4E and PUF3 effects on deadenylation were shown, in turn, to necessitate a func

291 en combined with ccr4Delta, severely blocked deadenylation when coupled with a caf1 deletion.

292 hanisms: repression by Puf4p is dependent on deadenylation, whereas repression by Mpt5p can occur thr

293 which binds only to monomeric Orb2, promotes deadenylation, whereas the putative poly(A) binding prot

294 In mammalian cells, mRNA decay begins with deadenylation, which involves two consecutive phases med

295 However, little is known about rice mRNA deadenylation, which is an important regulation step of

296 mRNA degradation is often initiated by deadenylation, which leads to decapping and 5'-3' decay.

297 ne major pathway for mRNA turnover occurs by deadenylation, which leads to decapping and subsequent 5

298 ge abundance is a consequence of accelerated deadenylation, which leads to rapid mRNA decay.

299 F1b target shared and unique transcripts for deadenylation with temporal specificity.

300 efore complete deadenylation, and disrupting deadenylation with use of an internal polyadenylate tail