ライフサイエンスコーパス: deamidate

1 tion for the peptide in which the residue is deamidated.

2 The deamidated 33-mer peptide efficiently exchanges with a p

3 At pH 5.5 and 7.3, dissociation of the deamidated 33-mer peptide from DQ2 is much slower than d

4 In addition, the deamidated 3C protease was found to be present in vivo,

5 OspI from enteric bacteria Shigella flexneri deamidates a glutamine residue in the host ubiquitin-con

6 a coli, acts on Rho-GTPases and specifically deamidates a single glutamine residue (Gln-63 in RhoA) r

7 ytic triad, which inactivates Rho GTPases by deamidating a conserved asparagine in the GTPase switch-

8 activates small GTPases of the Rho family by deamidating a single amino acid within these target prot

9 -tTG(553-564)-NH2 sequence cross-linked with deamidated Ac-alpha2-Glia(63-71)-NH2, was able to identi

10 Human WT-alphaA and human deamidated alphaA (alphaA-N101D, alphaA-N123D, alphaA-N1

11 deletion of the C-terminal extension in the deamidated alphaA mutants, but on N-terminal domain dele

12 xchange rate than heteroaggregate containing deamidated alphaA- and WT-alphaB subunits.

13 alphaA-crystallin, the lenses containing the deamidated alphaA-crystallin also showed an aggregation

14 idence for the first time that expression of deamidated alphaA-crystallin caused disruption of fiber

15 the heteroaggregate containing WT-alphaA and deamidated alphaB subunit showed lower chaperone activit

16 Deamidated alphaB-crystallin mutants (N78D, N146D, and N

17 The triply deamidated analog also aggregated into amyloid fibrils f

18 ic enzyme activity, recruit cellular PFAS to deamidate and activate RIG-I.

19 d enabled functional distinction of relevant deamidated and glycosylated proteoforms and the simultan

20 o play a role in the repair or metabolism of deamidated and isomerized proteins that are spontaneousl

21 Pup (prokaryotic ubiquitin-like protein) is deamidated and isopeptide linked to proteins by a mechan

22 lls with promiscuous recognition of both the deamidated and native epitopes and reduced frequency of

23 luten-reactive T cells in children recognize deamidated and native gluten epitopes, whereas T cells f

24 laevis oocytes were microinjected with both deamidated and nondeamidated forms of recombinant chicke

25 dic (lower pI) peaks were found to represent deamidated and sialyated species.

26 a highly conserved cysteine proteinase that deamidates and inactivates the anticancer drug bleomycin

27 physiological roles for the two alternative, deamidating and nondeamidating, routes of nicotinamide s

28 The deamidated antibody variants were less potent in antigen

29 esidues at positions 79, 141 and 187 and one deamidated Asn residue at position 176, were detected at

30 irmed, by mass spectroscopy, the presence of deamidated (Asp(64)) and native (Asn(64)) COMP epitopes

31 L-ASP from Escherichia coli deamidates asparagine (Asn) and glutamine, with an ~10(4

32 MM28, MM50, and Mel202 cells failed to deamidate Bcl-x(L) in response to radiation-induced DNA

33 Additionally, we show that degradation of deamidated Bcl-xL is mediated at least in part by calpai

34 71 and Asn94 react; these are more than half deamidated before Asn34 reacts, and its deamidation is e

35 of ubiquitination and degradation of WT and deamidated betaB1 crystallins were rapid and showed litt

36 ive 10-mer peptides were inactive, even when deamidated, but V96F substitution of deamidated TFIIA am

37 aragine residues 246 and 259 were completely deamidated by age 7 years.

38 Second, they are recognized and deamidated by human tissue transglutaminase (tTGase) wit

39 astrointestinal digestion and that have been deamidated by tissue transglutaminase.

40 TG2 deamidates certain gluten peptides, increasing their aff

41 d native (Asn(64)) COMP epitopes (mean 0.95% deamidated COMP (D-COMP) relative to native COMP) in car

42 Wild-type (WT) and deamidated crystallins were expressed and (125)I-radiola

43 d residue Asn55 in vivo and the ratio of the deamidated derivatives, i.e., isoAsp55 and Asp55.

44 the pathogenesis of celiac disease (CeD) by deamidating dietary gluten peptides, which facilitates a

45 t cleavage propensities were revealed at the deamidated DN site compared to the native NN motif for t

46 In conclusion, both Cp NGR sites can deamidate during aging under oxidative conditions, likel

47 ns are crystallins, and they are extensively deamidated during aging and cataracts.

48 he yeast Saccharomyces cerevisiae, which can deamidate either N-terminal asparagine or glutamine.

49 mportant component of the disease, both as a deamidating enzyme that can enhance the immunostimulator

50 We believe that enrichment of deamidated epitope in hip OA cartilage indicates a lesse

51 ll development is reduced in response to the deamidated epitope, pointing to regulatory T-cell develo

52 tibodies to deamidated IA-2 were specific to deamidated epitopes and were predominantly present durin

53 ifically targeting reported T-cell reactive, deamidated epitopes of IA-2 and explore their relationsh

54 Recombinant deamidated ErA mutants were also demonstrated to migrate

55 In contrast, the hidden (962)NGR site can deamidate exclusively when aging occurs under oxidative

56 s protein (which is not associated with DNA) deamidated fairly readily in spores (t(1/2), approximate

57 is indicated that the pI 8.3 protein was the deamidated form of 3C, and it displayed approximately 10

58 ne immunogenicity, we created a "genetically deamidated" form of rPA using site-directed mutagenesis

59 ), and gammaS (52-71)) and their potentially deamidated forms as model peptides.

60 HPLC isolation of various deamidated forms followed by peptide mapping and mass sp

61 identify and quantitate the amidated versus deamidated forms of each tryptic fragment of alpha-A cry

62 of tryptic fragments containing amidated and deamidated forms using high pressure liquid chromatograp

63 tic-tryptic digest of gliadin (in native and deamidated forms) before T-cell collection.

64 ntified omega- and gamma-gliadins, and their deamidated forms, as immunodominant B-cell epitopes in w

65 n of stem cell factor dimer, a total of five deamidated forms, including two homodimers and three het

66 or the initiation of celiac disease in which deamidated free human peptides with T-cell epitope homol

67 exhibit cross-reactivity between native and deamidated GAD65 epitopes, regulatory T-cell development

68 observed that TCRs reactive to the native or deamidated GAD65115-127 led to efficient development of

69 ELISA, all the sera displayed IgE binding to deamidated gamma- and omega2-gliadins and deamidated tot

70 TG and total IgA, or IgA-TTG and IgG against deamidated gliadin (IgG-DGL) could identify patients wit

71 negative tissue anti-transglutaminase and/or deamidated gliadin antibodies in their medical records.

72 Measuring total IgA levels, IgG deamidated gliadin antibody tests, and TG2-IgG testing i

73 tests against tissue transglutaminase (TG2), deamidated gliadin peptide (DGP) and endomysium (EMA).

74 d as celiac disease antibody positivity (IgG-deamidated gliadin peptide above 10.0 U/mL and/or IgA-ti

75 subset of study participants, mean levels of deamidated gliadin peptide autoantibodies were 7.46 (6.9

76 bodies against tissue transglutaminase-2 and deamidated gliadin peptide), symptom frequencies, and sa

77 or anti-tissue transglutaminase, IgA against deamidated gliadin peptide, and endomysial antibody (IgA

78 tibodies against tissue transglutaminase and deamidated gliadin peptide, greatly facilitate diagnosis

79 n and IgG against tissue transglutaminase or deamidated gliadin peptide, or endomysial antibody, shou

80 iomarkers of CeD derived from neoepitopes of deamidated gliadin peptides (DGP) and tTG fragments and

81 The detection of IgG against deamidated gliadin peptides (DGP) has high specificity a

82 ased on the detection of serum antibodies to deamidated gliadin peptides (DGPs).

83 antibodies against native gliadin (ngli) and deamidated gliadin peptides (dpgli), as well as for IgA

84 Novel deamidated gliadin peptides antibodies have better diagn

85 utamic acid residues of the TG2 epitope with deamidated gliadin peptides could be a structural basis.

86 or TGA-IgA, 1 for TGA-IgG, 6 for IgG against deamidated gliadin peptides, and 1 for EMA, from 5 diffe

87 c antigens tissue transglutaminase (tTG) and deamidated gliadin, and the classifier accuracy was inde

88 lmark antigens tissue transglutaminase-2 and deamidated gliadin, exhibiting 71% sensitivity and 99% s

89 mysium, as well as IgA and IgG antibodies to deamidated gliadine peptide and TG6 and performed HLA an

90 mmunofluorescence assay, and antibodies to a deamidated gliadine peptide using an immunofluorometry a

91 ve to IgA antibodies to TG2, endomysium, and deamidated gliadine peptide.

92 It can be evidenced by strong IgE binding to deamidated gliadins or peptides of the type QPEEPFPE.

93 By deamidating Gln40 of Nedd8 to glutamate (Q40E), the bact

94 glutamine-50, and glutamine-147 residues, or deamidated glutamic acid residues at the same positions.

95 talyses the formation of protein crosslinks, deamidating glutamine in a side-reaction.

96 the consumption of food products containing deamidated gluten (DG) in subjects tolerant to wheat.

97 characterized by CD4(+) T cells recognizing deamidated gluten and by antibodies reactive to gluten o

98 ted from cultures with reactivity to complex deamidated gluten antigen or by sorting with gluten pept

99 Rs, TCRs specific for two immunodominant and deamidated gluten epitopes (DQ2.5-glia-alpha1a and DQ2.5

100 -dependent response of CD4(+) T cells toward deamidated gluten peptides in the intestinal mucosa of i

101 tudies have revealed that in addition to the deamidated gluten peptides themselves, their correspondi

102 , whereas T cells from adults only recognize deamidated gluten peptides.

103 tive and heteroclitic (stronger) response to deamidated gluten peptides.

104 Levels of T-cell responses were higher to deamidated gluten than to native gluten in children and

105 CD4(+) eT(effs) exposed to deamidated gluten through oral gavage colocalized with d

106 proteins on antigen-presenting cells (APCs), deamidated gluten-derived peptides, and T cell receptors

107 Moreover, data suggest that deamidated gluten-induced adaptive immunity is a suffici

108 -adenosylhomocysteine, increases the rate of deamidated HA-CaM degradation.

109 The experiments indicate that deamidated HA-CaM is degraded after microinjection, whil

110 Enzymatic methylation of deamidated HA-CaM with purified PIMT prior to injection

111 Newly identified autoantibodies to deamidated IA-2 are new biomarkers of islet autoimmunity

112 ponse to post-translationally modified (PTM) deamidated IA-2 peptides; autoantibodies to these PTM ne

113 Autoantibodies to deamidated IA-2 were specific to deamidated epitopes and

114 An asparagine known to be deamidated in human cataract is located in a highly orde

115 ase A, and a tryptic peptide from calmodulin deamidated in its native state.

116 Total spore core protein also deamidated in vivo, although the rate was two- to threef

117 phaAN101D-transgene and is considered to be "deamidated" in this study.

118 Mechanistically, CTPS1 interacts with and deamidates interferon regulatory factor 3 (IRF3).

119 dation accelerates amyloid formation and the deamidated material is able to seed amyloid formation by

120 se CheD deamidates Q609 in McpC and does not deamidate McpB.

121 owing posttranslationally phosphorylated and deamidated, modified sites in the bovine MBP components

122 transglutaminase 2 (TGM2), which selectively deamidates multiple glutamine residues on p21, stabilizi

123 A selectively deamidated multivalent peptide from gluten (LQLQPFPQPELP

124 inantly expressed wild-type betaB1 (WT), the deamidated mutant (Q204E), an N-terminally truncated mut

125 llins, and (iii) WT-alphaA:individual alphaB-deamidated mutant crystallins.

126 on deletion of the N-terminal domain in the deamidated mutant proteins, but opposite effects were ob

127 Our results indicated that the deamidated mutant was significantly destabilized relativ

128 These structural changes in the deamidated mutants led to decreased stability during unf

129 ected mutagenesis was used to generate three deamidated mutants of alphaB-crystallin: N78D, N146D, an

130 ompared with the WT-alphaA, the three alphaA-deamidated mutants showed reduced levels of chaperone ac

131 T-alphaB crystallins, (ii) individual alphaA-deamidated mutants:WT-alphaB crystallins, and (iii) WT-a

132 mpered the oxidized peptides rather than the deamidated ones during ripening.

133 mploying CTPS1 conditional deletion and IRF3 deamidated or deamidation-resistant knockin mice, we dem

134 In addition, we present evidence that CheD deamidates other B. subtilis chemoreceptors including Mc

135 nd NR, respectively, connecting amidated and deamidated pathways.

136 Further, a deamidated peptide from the Ig2-domain of IgLON5 activat

137 -acid peptide corresponding to the partially deamidated peptide of A-gliadin amino acids 57-73 (Q65E)

138 d the unprecedented quantitative analysis of deamidated peptide products arising from the mAb heavy c

139 amidation and suggest that both Gln- and Asn-deamidated peptides can promote the activation and expan

140 es responsive against one Gln- and three Asn-deamidated peptides could be isolated from peripheral bl

141 structs containing cross-linked tTG and Glia deamidated peptides have been synthesized.

142 ween the frequencies of T cells specific for deamidated peptides, insulin antibody levels at diagnosi

143 epitopes and many have higher reactivity to deamidated peptides.

144 residues 140-173) deleted alphaA mutants and deamidated plus N-terminal domain or C-terminal extensio

145 w that fibers formed by samples that contain deamidated polypeptide contain reduced amounts of beta-s

146 sensitive signature peptide (IYPTNGYTR), its deamidated products (IYPTDGYTR and IYPTisoDGYTR), and a

147 1D patient could only be generated against a deamidated proinsulin peptide, but cross-reacted with na

148 cable to defining the isomerization state of deamidated proteins.

149 cts even for those peptides showing multiple deamidated proteoforms.

150 e between the chemoreceptors is because CheD deamidates Q609 in McpC and does not deamidate McpB.

151 ponses, tissue transglutaminase specifically deamidated Q65 in the peptide of A-gliadin amino acids 5

152 A gamma-type SASP of B. subtilis also deamidated readily in vitro (t(1/2) for one net deamidat

153 CheD not only deamidates receptor glutamine residues contained within

154 fuels de novo nucleotide synthesis but also deamidates RelA.

155 The characterization of Asn deamidated residues by LERLIC-MS/MS also uncovered novel

156 oop deletions or wild-type CNF1-C is able to deamidate RhoA containing Asn-63 instead of Gln-63, sugg

157 quired for enzymatic activity of the toxins, deamidates RhoA and Cdc42 better than CNF2.

158 hrome c, a tryptic peptide from unfolded and deamidated ribonuclease A, and a tryptic peptide from ca

159 arboxyl-terminal fragment were sufficient to deamidate RIG-I in vitro.

160 Purified PFAS and viral homolog thereof deamidate RIG-I in vitro.

161 cotinate was depleted and metabolites of the deamidated salvage pathway were reduced but intracellula

162 approximately 1 h at 70 degrees C), and the deamidated SASP no longer bound to DNA effectively.

163 genesis requires transglutaminase 2 (TG2) to deamidate select glutamine residues in diet-derived glut

164 the CheC/CheD/CheYp adaptation system and to deamidate selected residues to activate the chemorecepto

165 translationally modified peptides, including deamidated sequences.

166 ariant SOD1 (E100K, E100G, D90A), and triply deamidated SOD1 (modeled with N26D/N131D/N139D substitut

167 ned positions into gluten T-cell epitopes by deamidating specific glutamine residues on the basis of

168 on priming, both the native-specific and the deamidated-specific T cells accumulated in pancreatic is

169 midation, the sequence Asn(45)-Gly(46) being deamidated spontaneously at near-neutral and basic pH an

170 d WT-alphaB, the heteroaggregates containing deamidated subunits of either alphaA- or alphaB-crystall

171 en when deamidated, but V96F substitution of deamidated TFIIA amino acid residues 91-100 stimulated I

172 r was much less efficiently demethylated and deamidated than intact receptor, but essentially was unp

173 ne synthesis, and was recently identified to deamidate the NF-kappaB subunit RelA to promote aerobic

174 iting factors (Cifs) into mammalian cells to deamidate the ubiquitin-like protein NEDD8.

175 Dop (deamidase of Pup; Mtb Rv2112c/MT2172) deamidates the C-terminal glutamine of Pup to glutamate,

176 ues in Cu, Zn superoxide dismutase (SOD1) to deamidate to aspartate remains uncharacterized; its occu

177 a variant form of beta(2)M in which Asn17 is deamidated to Asp (N17D).

178 pectrometry demonstrated that ~23% of N26 is deamidated to aspartate (iso-aspartate was undetectable)

179 ealed that a single asparagine, Asn-164, was deamidated to aspartic acid in the pI 8.3 isoform.

180 thesized with a terminal glutamine, which is deamidated to glutamate by Dop (deamidase of Pup) prior

181 NMN produced by either route 2 or route 3 is deamidated to nicotinic acid mononucleotide and converte

182 ing residues, in that they are enzymatically deamidated to yield secondary destabilizing residues asp

183 to deamidated gamma- and omega2-gliadins and deamidated total gliadins, frequently with high concentr

184 artyl detection in peptides from proteins; a deamidated tryptic peptide of cytochrome c, a tryptic pe

185 observed that the exposed (568)NGR site can deamidate under conditions mimicking accelerated Asn agi

186 in the helicase 2i domain of RIG-I that were deamidated upon UL37 expression or HSV-1 infection.

187 volved in the first step of the two-step non-deamidating utilization of nicotinamide (NMN shunt).

188 trate for the carboxylmethyltransferase, but deamidated variants of calmodulin act as substrates for

189 D4/DQ8 mice also responded well to modified (deamidated) versions of the gliadin peptides, whereas HC

190 that confer loss of function phenotypes when deamidated via site-specific mutation.

191 es 481 and 501 in the receptor-binding motif deamidate with a half-life of 16.5 and 123 days at 37 C,

192 it is bound to DNA, the alpha/beta-type SASP deamidated with a t(1/2) of 2 to 3 h at 95 degrees C.