ライフサイエンスコーパス: debranching

1 tuations in mother filament shape influenced debranching.

2 tween the roles of DBR1 in transposition and debranching.

3 loidin, to stabilize actin filaments against debranching.

4 phosphate dissociation, ADF/cofilin promotes debranching.

5 ns appear to be tailless lariats that escape debranching.

6 ways of branch formation, stabilization, and debranching.

7 the branch junction is sufficient to trigger debranching.

8 e times to F-actin branch junctions prior to debranching.

9 the release of free glucose through glycogen debranching.

10 d in a circular form due to defective lariat debranching.

11 hat GMF depends on two separate surfaces for debranching.

12 2/3 complex at branch junctions and promotes debranching.

13 ddressed which factors promote actin network debranching.

14 requires manganese as the metal cofactor for debranching.

15 of 70 patients (24%): chimney, 3; open iliac debranching, 1; coiling, 8; onyx, 3; and chimney plus on

16 its filament-severing activity and that the debranching activities of the two proteins are additive.

17 Finally, we show that this debranching activity and mechanism are conserved for mam

18 e branchpoint junction were shown to inhibit debranching activity and, hence, represent "decoys" for

19 antly nuclear Dbr1 enzyme to encode the sole debranching activity in human cells.

20 3 to alanine abolishes or greatly diminishes debranching activity in vitro.

21 The debranching activity of MICAL1 could be involved beyond

22 ic proteins whose degradation depends on the debranching activity of UCH37.

23 SI N-terminal subunit has an additional exo-debranching activity on the alpha-1,6-linkage.

24 n accumulation when the only isoamylase-type debranching activity present is ISA1 homomer, but not in

25 a human data set acquired in the presence of debranching activity, LaSSO identified both canonical an

26 reducing aggregation but is dispensable for debranching activity.

27 e investigated which enzymes are involved in debranching amylopectin during transient starch degradat

28 ts of Northern, ribozyme, RT-PCR, and lariat debranching analyses indicate that the two species are c

29 g RNAs that are typically destined for rapid debranching and degradation.

30 amma phosphate, ADF/cofilin proteins promote debranching and depolymerization.

31 Therefore, the combination of debranching and infrared treatment could be an efficient

32 GMF is implicated in both Arp2/3 debranching and inhibition of Arp2/3 activation.

33 -specific binding site is required for chain debranching and proteasome-mediated degradation of prote

34 rom native pea starch through acid thinning, debranching and recrystallization, and the resultant pea

35 cal feature of dendritic nucleation in which debranching and subsequent actin-filament remodelling an

36 entity of the molecular mechanism underlying debranching and whether this activity extends to mammali

37 prevented annealing of short filaments after debranching and, with profilin, allowed filaments to dep

38 at alpha-glucosidase activity, i.e. glycogen debranching and/or lysosomal glycogen breakdown, contrib

39 d into pre-miRNA hairpins after splicing and debranching, and miRNAs can also be excised by Dicer cle

40 beta-glucosidases, xylosidases, carbohydrate-debranching, and pectin-acting enzymes-all of which indi

41 iogenesis pathway involving splicing, lariat debranching, and RNA exosome-mediated "trimming," follow

42 as enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes.

43 Debranching appears to be a rate-limiting step for the t

44 Debranching appears to be catalyzed by two different mem

45 Network disassembly and debranching appears to be linked to actin-bound ATP hydr

46 sing oligomers, indicating that severing and debranching are important steps in the disassembly proce

47 However, their mechanisms of debranching are only partially understood.

48 diester bonds was verified using an in vitro debranching assay.

49 om hairpins excised by splicing, which after debranching become substrates for Dicer and load into RI

50 isiae coronin (Crn1), enhances Gmf1-mediated debranching by 8- to 10-fold, and that these effects dep

51 op structure that is hypothesized to prevent debranching by cellular enzymes.

52 vations suggest that GMF and cofilin promote debranching by distinct yet complementary mechanisms.

53 ith ADP-Arp2/3 complex are more sensitive to debranching by fission yeast GMF (glia maturation factor

54 er physiological ionic conditions to observe debranching by GMF and cofilin.

55 These data suggest that debranching by GMFbeta plays an important role in branch

56 that the lariat-intron is not accessible to debranching by purified Dbr1 when it is held in the T123

57 RS3 yields can be substantially enhanced by debranching cassava starch using pullulanase followed by

58 Our work therefore defines UCH37 as a debranching deubiquitinase important for promoting prote

59 The debranching effect of pullulanase was stabilized by the

60 When the T7 gene 3-encoded DNA debranching endonuclease is absent during in vitro T7 DN

61 ensive network forms when the gene 3-encoded debranching endonuclease is present.

62 unction-specific resolvase can evolve into a debranching endonuclease tailored to the requirements of

63 of BE together with the starch synthases and debranching enyzmes were able to create crystallization-

64 D-III) is caused by a deficiency of glycogen debranching enzyme (AGL) activity.

65 investigations of group II intron splicing, debranching enzyme (Dbr) activity, and other biochemical

66 spliceosome must be hydrolyzed by the intron debranching enzyme (Dbr1) before they can be metabolized

67 tudies in our laboratory showed that the RNA debranching enzyme (DBR1) is not required for early step

68 rpin RNA (shRNA) knockdown of the RNA lariat debranching enzyme (DBR1) led to a decrease in the produ

69 One of these host factors is the RNA lariat debranching enzyme (Dbr1), which cleaves the 2'-5' bond

70 ive disease, is caused by deficient glycogen debranching enzyme (GDE) activity.

71 ns of the AGL gene encoding for the glycogen debranching enzyme (GDE).

72 Glycogen debranching enzyme (gene symbol, AGL) is a multifunction

73 Here, we report that the human RNA debranching enzyme (hDBR1), when inappropriately regulat

74 Functions of isoamylase-type starch-debranching enzyme (ISA) proteins and complexes in maize

75 ubunit of heteromeric isoamylase-type starch-debranching enzyme (ISA1/ISA2 heteromer).

76 tein body and found to be enriched in starch debranching enzyme (pullulanase).

77 Inherited deficiency of the RNA lariat-debranching enzyme 1 (DBR1) is a rare etiology of brains

78 which inborn errors of the human RNA lariat-debranching enzyme 1 (DBR1) underlie brainstem viral enc

79 been named AtDBR1 (for Arabidopsis thaliana Debranching enzyme 1).

80 pes of alpha(1-->6) glucan hydrolase (starch-debranching enzyme [DBE]).

81 n accumulation when the only isoamylase-type debranching enzyme activity present is ISA1/ISA heterome

82 These intronic sequences can escape the debranching enzyme and accumulate as lariats.

83 Although the ex vivo activities of debranching enzyme and lysosomal acid maltase, two major

84 form of a lariat and rapidly targeted by the debranching enzyme and nuclear exonucleases for lineariz

85 osin binding protein C (C-protein), glycogen debranching enzyme and ryanodine receptor 2 were also id

86 These data indicate that isoamylase-type debranching enzyme and SSIII work in a coordinated fashi

87 structure of the complex between the starch debranching enzyme barley limit dextrinase (LD), hydroly

88 t an enzyme of analogous nature to the plant debranching enzyme but of a different bacterial origin w

89 Saccharomyces cerevisiae, the absence of the debranching enzyme causes these lariat RNAs to accumulat

90 he 2',5'-bonds must be hydrolyzed by the RNA debranching enzyme Dbr1 before spliced introns can be de

91 A new study shows that loss of the lariat debranching enzyme Dbr1 suppresses TDP-43 toxicity.

92 Using fission yeast cells lacking the debranching enzyme Dbr1, LaSSO not only accurately ident

93 TDN1 C-terminal region directly binds lariat debranching enzyme DBR1, whereas its N-terminal intrinsi

94 on in DBR1 (p.D262Y) encoding the RNA lariat-debranching enzyme DBR1, which is involved in the remova

95 ng new BP data from RNA sequencing of lariat debranching enzyme DBR1-mutated patients and from machin

96 ' linkage of these lariats is the RNA lariat debranching enzyme Dbr1.

97 lariat stability by knocking down the lariat debranching enzyme Dbr1.

98 We now report the implication of starch debranching enzyme in the aggregation of semicrystalline

99 Lariat debranching enzyme is also necessary for siRNA productio

100 Because the debranching enzyme is conserved among eukaryotes, this a

101 indirect evidence that the heteromultimeric debranching enzyme ISA1-ISA2 is not involved in starch b

102 SS2, and SS3 (to vary chain lengths) and the debranching enzyme ISOAMYLASE1-ISOAMYLASE2 (ISA; to alte

103 genes on SBEIIa, starch synthase, or starch-debranching enzyme isoforms were observed.

104 This involved the recruitment of a debranching enzyme of chlamydial pathogen origin.

105 This enzyme was identified as a debranching enzyme of the isoamylase type.

106 that gene expression of LIMIT DEXTRINASE1, a debranching enzyme that cleaves branch points within sta

107 yces cerevisiae Dbr1 is a 405-amino acid RNA debranching enzyme that cleaves the 2'-5' phosphodiester

108 ells lacking both SpPrp18 and SpDbr1 (lariat debranching enzyme), a genetic background suitable for d

109 haracterized by a deficiency in the glycogen debranching enzyme, amylo-1,6-glucosidase,4-alpha-glucan

110 haracterized by a deficiency in the glycogen debranching enzyme, amylo-1,6-glucosidase,4-alpha-glucan

111 G (type 1 phosphatase-targeting subunit), or debranching enzyme, making it unlikely that these protei

112 -fold increase in the activity of the starch debranching enzyme, pullulanase (limit dextrinase), the

113 I, SBEIIb, and sugary1, the putative starch-debranching enzyme, were each highly enriched in the amy

114 The csrA gene did not affect glycogen debranching enzyme, which is now shown to be encoded by

115 action of the splicing machinery and lariat-debranching enzyme, which yield pre-miRNA-like hairpins.

116 Pullulanase is a well-known starch-debranching enzyme.

117 cleavage of the 2',5' linkage by recombinant debranching enzyme.

118 eletion of DBR1, which encodes an RNA lariat debranching enzyme.

119 or to genes up-regulated by deletion of the debranching enzyme.

120 e show that c-di-GMP also binds the glycogen-debranching-enzyme, GlgX, uncovering a direct link betwe

121 tered by mutations of isoamylase-type starch-debranching enzymes (DBE), although how these proteins a

122 ific isoamylase- and pullulanase-type starch-debranching enzymes (DBEs) present in developing maize (

123 Isoamylase-type starch debranching enzymes (ISA) play important roles in starch

124 Conserved isoamylase-type starch debranching enzymes (ISAs), including the catalytic ISA1

125 e to alpha-(1-->6) glucan hydrolases (starch-debranching enzymes [DBEs]).

126 tide diversities, while pyrophosphatases and debranching enzymes are most conserved.

127 ity is that specific SSs, BEs, and/or starch debranching enzymes associate physically with each other

128 eals from a need for effective inhibition of debranching enzymes having characteristic open active si

129 r abundance of oligosaccharide degrading and debranching enzymes in buffalo rumen metagenome and that

130 our goal was to evaluate the role of starch debranching enzymes in the determination of the structur

131 The starch debranching enzymes isoamylase 1 and 2 (ISA1 and ISA2) a

132 addition of pregelatinized starch and starch-debranching enzymes produced by far the highest amount o

133 h/glycogen synthases, branching enzymes, and debranching enzymes) are differentially expressed in Mul

134 zyme families (oligosaccharide degrading and debranching enzymes) in digestion of coarse feed.

135 ene with sequence similarity to higher plant debranching enzymes, and both mutants lacked a chloropla

136 Previous studies identified two debranching enzymes, isoamylase 3 (ISA3) and limit dextr

137 ose pyrophosphorylase, starch synthases, and debranching enzymes, leading to varied chain lengths and

138 enes encoding granule-bound starch synthase, debranching enzymes, pullulanase, and starch phosphoryla

139 rotein with domains characteristic of lariat debranching enzymes, which has been named AtDBR1 (for Ar

140 pyrophosphorylase, and starch branching and debranching enzymes.

141 , starch branching enzymes (BEs), and starch debranching enzymes; however, the molecular explanation

142 and provide a molecular understanding of the debranching events associated with optimal starch mobili

143 nd fission yeast Arp2/3 complex and observed debranching events in real time with total internal refl

144 ains bound to the mother filament after most debranching events, even when accelerated by force.

145 tropomyosin-decorated networks just prior to debranching events.

146 Arp2/3 complex inhibitor and actin filament debranching factor, regulates lamellipodial protrusion d

147 non-canonical miRNAs arising by splicing and debranching from short introns.

148 Debranching further revealed higher retention of DP 11 -

149 d dbr1 mutant alleles are also deficient for debranching, further supporting a role for 2'-5' phospho

150 When debranching is blocked, these splicing intermediates can

151 Mortality after hybrid repair and visceral debranching is highly variable by center, but strongly a

152 Effective debranching is nevertheless achieved, as a result of coo

153 ester bond found in excised intron lariats ("debranching") is essential for turnover of intronic sequ

154 by a novel mechanism and that branching and debranching may play roles in Ty1 reverse transcription

155 ison of tiling array signals of RNA from the debranching mutant to the wild-type parent strain, and t

156 along with mutagenesis studies, suggest that debranching (not inhibition of Arp2/3 activation) is a p

157 we demonstrated that GMF potently stimulates debranching of actin filaments produced by Arp2/3 comple

158 One of the only factors known to promote debranching of actin networks is the yeast homolog of gl

159 plex plays a physiological role by promoting debranching of aged branch junctions without interfering

160 The results reveal the high debranching of arabinan side chains of RG I as compared

161 The debranching of both complex hemicellulosic and pectinace

162 Strikingly, cortactin potently inhibited the debranching of filament networks.

163 h can arise by either hydrolytic splicing or debranching of lariat RNA, cannot carry out both reverse

164 Debranching of OS starches was incomplete compared with

165 Thus, the debranching of RNA lariats by DBR1 permits G3BP1/2- and

166 ge (canonical substrates) or by splicing and debranching of short introns (mirtrons).

167 repairs using extra-anatomic, open surgical debranching of the renal-mesenteric arteries, followed b

168 Currently, branching and debranching of viral gRNA are not widely recognized as f

169 ackaging is thought to be dependent upon DNA debranching or other repair processes, and such events c

170 with a need for terminal repeat duplication, debranching, or damage repair concomitant with DNA packa

171 tin chains at similar rates and with similar debranching patterns, producing monoubiquitin species.

172 a maturation factor (GMF), which accelerates debranching, prevents branch renucleation.

173 in-5B and its receptor PlexinA1 regulate the debranching process.

174 ted proteasome complexes, we find that chain debranching promotes degradation of substrates modified

175 Quantitative comparison of debranching rates with the known kinetics of cofilin-act

176 entity of the branchpoint nucleotide affects debranching rates.

177 hiodystrophy nonphotosensitive 1 to in vitro debranching reactions increases the catalytic efficiency

178 Hybrid debranching repair of pararenal and thoracoabdominal aor

179 e reviewed the collective outcomes of hybrid debranching repairs using extra-anatomic, open surgical

180 To understand how UCH37 achieves its unique debranching specificity, we performed biochemical and Nu

181 TP state, and nucleotide hydrolysis promotes debranching, suggesting that the higher affinity of GMF

182 d be resolved during GMF- or cofilin-induced debranching, the Arp2/3 complex left the branch junction

183 Debranching this RNA in vitro with Dbr1p creates an unca

184 ma-phosphate from ADP-Pi-actin filaments and debranching to 30 seconds.

185 By debranching ubiquitin chains composed of K48 linkages, t

186 mplex polymerization but effectively induces debranching, whereas the isolated beta1beta2 or CA domai

187 ormation in the cochlea is a process of SGN "debranching" whereby SGNs lose extraneous branches befor