ライフサイエンスコーパス: deep sequencing

1 ariants more accurately compared to standard deep sequencing.

2 ion is further validated through target site deep sequencing.

3 ion, cDNA generation, PCR amplification, and deep sequencing.

4 ulation, barcoded 16S rRNA amplification and deep sequencing.

5 onstruction of a strand-specific library for deep sequencing.

6 fer, USA, by both 16S rDNA and rRNA amplicon deep sequencing.

7 ing the ribosome-protected mRNA fragments to deep sequencing.

8 ous system-wide presynaptic transcriptome by deep sequencing.

9 q, for measuring RNA structure in cells with deep sequencing.

10 re determined by immunohistochemistry and/or deep sequencing.

11 t enable their delivery to be quantified via deep sequencing.

12 f blood DNA by polymerase chain reaction and deep sequencing.

13 e complex samples of human hippocampus using deep sequencing.

14 that could be tracked using next-generation deep sequencing.

15 scle) can be quantified simultaneously using deep sequencing.

16 arbored S282C/T RASs at treatment failure by deep sequencing.

17 ta using 16S ribosomal RNA (rRNA) gene V4-V5 deep sequencing.

18 riginal shotgun metagenomics method based on deep sequencing.

19 orescence-activated cell sorting followed by deep sequencing.

20 NKTCL patients, through whole-exome/targeted deep sequencing.

21 nally, ultimately enhancing the precision of deep sequencing.

22 with zero transposon integrations in HPRT by deep sequencing.

23 fied by analyzing reads from high-throughput deep sequencing.

24 imens for microRNA expression analysis using deep sequencing.

25 DNA to be sorted to recover long targets for deep sequencing.

26 s labeling, viral neutralization assays, and deep sequencing.

27 cant for their use as reagents including for deep sequencing.

28 on 16S rDNA and internal transcribed spacer deep sequencing.

29 man cardiac fibroblasts were analyzed by RNA deep sequencing.

30 [tRNA] fragments) across 11 mouse tissues by deep sequencing.

31 rmed, in addition to bulk exome and targeted deep-sequencing.

32 n (FOI) and rate of clearance using amplicon deep-sequencing.

33 d mouse subspecies over five generations and deep-sequencing 119 offspring, we detect thousands of cr

34 ted with the high diagnostic yield were: (1) deep sequencing (~2,000x coverage), (2) a broad gene set

35 constructed a cassava haplotype map through deep sequencing 241 diverse accessions and identified >2

36 na fide circular topology using circular DNA deep-sequencing, 2D gel electrophoresis and inverse poly

37 ssion atlas of miRNAs and their promoters by deep-sequencing 492 short RNA (sRNA) libraries, with mat

38 we describe NucleaSeq-nuclease digestion and deep sequencing-a massively parallel platform that measu

39 livary transcriptome of the N. neivai, using deep sequencing achieved by the Illumina protocol.

40 generation sequencing technology has allowed deep sequencing across related species and of individual

41 hose of pol II chromatin immunoprecipitation-deep sequencing across the chromosomal coordinates of sh

42 We perform deep sequencing across the DAP1 genomic segment in 2032

43 The unbiased nature of metagenomic RNA deep sequencing allowed an expanded scope of pathogen de

44 previous genomic analyses using ~50x depth, deep sequencing allowed us to identify a novel super-spr

45 Deep sequencing allowed us to identify intrahost single-

46 Immune repertoire deep sequencing allows comprehensive characterization of

47 Amplicon deep sequencing (Amp-Seq) offers a tool to address this

48 y genetic and biochemical studies as well as deep sequencing analyses, we find that AGO mutations dis

49 Here, integrating full-genome and deep-sequencing analyses, structural information, and in

50 inst permuted data and validated in targeted deep-sequencing analyses.

51 nts) of Mut268 compared to WT using targeted deep-sequencing analyses.

52 matin immunoprecipitation [ChIP] followed by deep sequencing) analyses in brown adipose tissue showed

53 ed a DNA- based multi-gene panel using ultra-deep sequencing analysis (including 14 genes with up to

54 yte miRNome and miRISC-associated miRNome by deep sequencing analysis of primary human chondrocytes.

55 s is poorly understood, we first performed a deep sequencing analysis of the V(D)J regions of VH and

56 ity to transmit by respiratory droplets, and deep sequencing analysis revealed that the H7N9 viruses

57 Furthermore, deep sequencing analysis reveals that ZIKV populations i

58 We expect GeDi to find use cases in targeted-deep sequencing analysis, and to serve as a replacement

59 Using deep sequencing analysis, we investigated miRNA expressi

60 Combining deep-sequencing analysis and our previously described in

61 We applied it to 185 deep sequencing and 90 assembled Han Chinese genomes and

62 Recent advances in technology have enabled deep sequencing and analysis of members and signals of t

63 EpiSC specific phage were analyzed by deep sequencing and bioinformatics analysis to identify

64 Using deep sequencing and case-control genotyping studies, we

65 ng polymerase chain reaction (PCR), targeted deep sequencing and comprehensive analysis.

66 s) and Vero (primate) cells, with subsequent deep sequencing and computational analyses of recovered

67 Notably, RNA deep sequencing and functional analyses in HuR-deficient

68 ined with chromatin immunoprecipitation with deep sequencing and functional characterization conducte

69 Here, by combining deep sequencing and genomic and genetic approaches in Ca

70 Deep sequencing and in situ hybridization suggest that H

71 sed RNAs were identified in urine samples by deep sequencing and metabolites in urine were measured b

72 affect caste in eusocial evolution, we used deep sequencing and Northern blots to isolate caste-asso

73 advances in genomics, increasingly accurate deep sequencing and novel methods of cell tracking have

74 Using a combination of deep sequencing and PCR-based screening for the presence

75 The confluence of deep sequencing and powerful machine learning is providi

76 1 chromatin immunoprecipitation coupled with deep sequencing and transcriptome analysis upon FOXA1 kn

77 ing in vivo and in vitro integration assays, deep sequencing, and exonuclease footprinting, we show t

78 eptides were identified by phage display and deep sequencing, and hits were characterized by electrop

79 ndom sequences from a phage display library, deep sequencing, and pattern analysis to define the crit

80 tegrated approach combining viral passaging, deep sequencing, and protein structural analyses to defi

81 We used a sensitive gene-targeted deep sequencing approach to gain precision on the exact

82 We used a deep-sequencing approach, together with neutralization p

83 without a history of periodontitis through a deep-sequencing approach.

84 microbiome after treatment with EMD using a deep-sequencing approach.

85 Using deep sequencing approaches, we discovered that a member

86 sing both in silico data mining and targeted deep-sequencing approaches.

87 sing a quantitative, continuous-culture-with-deep-sequencing assay, we confirm that the top two SCA-p

88 Deep sequencing based ribosome footprint profiling can p

89 Whole-genome deep sequencing-based analysis has revealed unexpectedly

90 We applied 5'RACE and a deep sequencing-based approach to investigate the clonal

91 ations were identified for three lines using deep sequencing-based bulked segregant analysis, and in

92 Deep sequencing can detect somatic DNA mutations in tiss

93 Metagenomic RNA deep sequencing can reliably detect and quantify common

94 We investigate whether 'deep' sequencing can provide additional resolution compa

95 Here we demonstrate how viral deep-sequencing can be used to reconstruct HIV-1 transmi

96 We performed deep sequencing (CAncer Personalized Profiling by deep S

97 hnologies (bisulfite conversion, followed by deep sequencing) cannot distinguish between 5mC and 5hmC

98 ovements to cancer personalized profiling by deep sequencing (CAPP-Seq)(5), a method for the analysis

99 sequencing (CAncer Personalized Profiling by deep Sequencing, [CAPP-Seq]) analyses of plasma cell-fre

100 HJ chromatin immunoprecipitation with deep sequencing (ChIP-seq) analysis showed the direction

101 Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) and ChIP-reChIP-seq analyses

102 Chromatin immune precipitation followed by deep sequencing (ChIP-seq) and POLR2A chromatin interact

103 Using EHF ChIP followed by deep sequencing (ChIP-seq) and RNA sequencing after EHF

104 ad chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) signals for H3K27ac or other

105 , chromatin immune precipitation followed by deep sequencing (ChIP-seq), chromatin interaction analys

106 slinking and immunoprecipitation followed by deep sequencing (ChIP-seq), we found that DnaA was assoc

107 as chromatin immunoprecipitation followed by deep sequencing (ChIP-seq).

108 Targeted deep sequencing combined with single-cell sequencing of

109 Deep sequencing confirmed excellent library balance (5.1

110 ant analysis in SC x SI F2 individuals using deep sequencing confirmed that all SC plants were S1 hom

111 Targeted deep-sequencing confirmed AS-PCR findings, and identifie

112 y, called Adaptive Base Error Model in Ultra-deep Sequencing data (ABEMUS), which combines platform-s

113 mes, using mutational profiles from targeted deep sequencing data acquired from 1,352 patients across

114 Batch effect is a frequent challenge in deep sequencing data analysis that can lead to misleadin

115 ase of fusion genes encompassing analysis of deep sequencing data and manual curations.

116 Lymphocyte antigen receptor repertoire deep sequencing data can be used to assess the clonal ri

117 ell lines to their corresponding mtDNA using deep sequencing data from two independent studies.

118 large-scale mapping of the branchpoints from deep sequencing data in three different species and in t

119 Deep sequencing data of airway biopsies are needed to ta

120 gration of chromatin immunoprecipitation and deep sequencing data sets with RNA sequencing revealed t

121 ing each individual's germline gene set from deep sequencing data, and show that it improves upon exi

122 With the rapidly increasing volume of deep sequencing data, more efficient algorithms and data

123 signed to archive the fusion candidates from deep sequencing data.

124 Deep-sequencing data provided additional diagnostic info

125 We have collected 1493 small RNA deep sequencing datasets and mapped a total of 5.5 billi

126 chment PCR, and sequencing by using multiple deep sequencing datasets.

127 a new approach for determining thresholds in deep-sequencing datasets of short RNA transcripts.

128 Metagenomic RNA deep sequencing detected pathogens in 86% (12/14) of the

129 Deep-sequencing experiments reveal highly specific, geno

130 Deep sequencing facilitated identification of highly exp

131 Deep sequencing for BTK and PLCG2 was performed retrospe

132 Using chromatin immunoprecipitation and deep sequencing for markers of active chromatin in human

133 Here, high-resolution ChIP/deep sequencing from infected primary effusion lymphoma

134 In this study, deep sequencing, genetic linkage analysis, and transcrip

135 By using deep sequencing, genome mapping and manual curations, we

136 pture of global RNA interactions with DNA by deep sequencing (GRID-seq) of fixed permeabilized nuclei

137 To this end, we used deep sequencing (GRO-seq and PRO-seq) and analyzed nasce

138 Targeted deep sequencing has been used widely as a diagnostic too

139 Recently, ultra-deep sequencing has enabled accurate measurement of VAFs

140 q) and high-throughput insertion tracking by deep sequencing (HITS).

141 Deep sequencing identified numerous mutant clones with m

142 Additional ultra-deep sequencing identified recurrent mutations in PKD1,

143 Finally, deep sequencing illustrates DrBHV super-infections in in

144 Here we perform deep sequencing in 360 primary breast cancers and develo

145 erformed FoxO1 chromatin immunoprecipitation-deep sequencing in mouse hearts after 7 days of isoprote

146 A combination of Primer-ID and deep sequencing is a promising approach that may quantif

147 However, unless extremely deep sequencing is performed on a very large number of i

148 t with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and h

149 be incorporated into transmission analyses; deep sequencing may facilitate more accurate detection o

150 his study, we apply unbiased metagenomic RNA deep sequencing (MDS) to identify pathogens causing conj

151 ssess circulating tumor DNA (ctDNA) by ultra-deep sequencing (median, 105,000x) of plasma DNA.

152 ere we adopted a newly developed tag linkage deep sequencing method and analyzed the quasispecies of

153 The amplicon-based deep sequencing method was used in the 3 parts of the st

154 Error analysis of the deep-sequencing method demonstrated reliable detection o

155 e therefore developed a culture-independent, deep-sequencing method targeting the 5' untranslated reg

156 Using a deep-sequencing method to measure chromosomal exonucleol

157 e and related questions, we here introduce a deep-sequencing methodology for studying RNA primer exte

158 Using specialized 3' end deep sequencing methods, we undertook a comprehensive an

159 Current deep-sequencing methods cannot distinguish TEELs from po

160 on, or (iii) technical errors/limitations of deep-sequencing methods.

161 h paired tumor/germline DNA) and/or targeted deep sequencing (n = 24) showed recurrent mutations of e

162 mutations in NTRK2 and NTRK3 identified via deep sequencing of 185 patients with hematological malig

163 We performed targeted ultra-deep sequencing of 25 genes and Digital Droplet PCR of T

164 Deep sequencing of 91 accessions identifies selective sw

165 w to allocate the limited sequencing budget: deep sequencing of a few cells or shallow sequencing of

166 es during postnatal maturation by performing deep sequencing of accessible chromatin regions by using

167 ext-generation sequencing assay for targeted deep sequencing of all exons and selected introns of 468

168 Deep sequencing of amplicon libraries from genomic DNA o

169 l genetic information from single-genome and deep sequencing of blood and tissue samples to investiga

170 he prognostic and predictive impact of ultra-deep sequencing of cell-free DNA in patients before and

171 Ds in mouse embryonic fibroblasts (MEFs) via deep sequencing of chromatin associated with purified nu

172 ally those with advanced metastatic disease, deep sequencing of circulating cell free DNA (cfDNA) obt

173 rtion site and content of transgenes through deep sequencing of genomic loci linked to specific known

174 Deep sequencing of HIV reverse transcriptase (RT) was pe

175 In conclusion, 454-deep sequencing of liver and plasma compartments in trea

176 Deep sequencing of lymphocyte receptor repertoires has m

177 miR-seq (deep sequencing of miRNAs) data reveal that the degradat

178 rol is ribosome profiling, which is based on deep sequencing of mRNA fragments protected from ribonuc

179 Deep sequencing of nasopharyngeal samples produced parti

180 Targeted deep sequencing of parental fibroblasts revealed that mo

181 Our study highlights the power of data from deep sequencing of pathogens as a component of outbreak

182 Using information from deep sequencing of patients with neurological or psychia

183 n pluripotent cells in vivo, obtained by the deep sequencing of pre-implantation embryos.

184 We validated these findings through deep sequencing of pulldown regions and whole-genome seq

185 Ultra-deep sequencing of resistant parasites identifies the sa

186 Deep sequencing of ribosomal DNA (rDNA) suggests thousan

187 It requires deep sequencing of ribosome protected mRNA fragments fol

188 es in mouse embryonic stem cells (mESCs) and deep sequencing of rRNA intermediates, we investigate th

189 Deep sequencing of size-selected DNase I-treated chromat

190 In the present study, we performed deep sequencing of small RNA molecules in the embryonic

191 By deep sequencing of small RNAs from Bactrocera dorsalis e

192 rase chain reaction-detected parasites using deep sequencing of SP-resistance alleles.

193 Deep sequencing of Spo11 oligonucleotides demonstrates t

194 pp1 and spp3 revealed they were allelic, and deep sequencing of spp3 identified an independent disrup

195 Deep sequencing of T-cell receptors enables the comprehe

196 bination of flow cytometric cell sorting and deep sequencing of the 16S rDNA gene was used to charact

197 phocytes in combination with improvements in deep sequencing of the autologous cancer have provided n

198 We propose that the information generated by deep sequencing of the BBCE cell lines coupled with phen

199 To address this question, we performed deep sequencing of the BCR repertoire of AChR-MG, MuSK-M

200 We performed high-throughput deep sequencing of the beta-TCR repertoire in 29 lesiona

201 Here, we perform deep sequencing of the CRISPRome-all spacers contained i

202 Deep sequencing of the HIV DNA partial env gene was perf

203 Deep sequencing of the Ig chain DNA was performed on iBA

204 Quantitative deep sequencing of the Ig heavy chain locus from B220(+)

205 ults demonstrate that affinity selection and deep sequencing of the phage library provide sufficient

206 horylation events in a systematic fashion by deep sequencing of the phosphoproteomes.

207 Full-genome deep sequencing of the plasmids, cultured viruses, and v

208 Deep sequencing of the repertoire of antigen receptor-en

209 Deep sequencing of the SLC26A9 gene in 762 individuals w

210 Deep sequencing of the tumor genome showed a highly hete

211 Primer ID deep sequencing of the V1/V3 region of the HIV-1 env gen

212 Deep sequencing of the viral episomes and host chromosom

213 Deep sequencing of transposon mutant libraries (or TnSeq

214 Deep sequencing of tRNA transcripts (tRNA-Seq) can estim

215 Ultra-deep sequencing of two healthy-looking skin biopsies ide

216 At the intrahost level, deep sequencing of viral DNA in original clinical sample

217 Next-generation deep sequencing of viral genomes, particularly on the Il

218 We performed deep sequencing of viral samples from patients chronical

219 Deep sequencing of viral samples illuminated the within-

220 Deep sequencing of virus from probang or tonsil swab sam

221 tensive structural diversity arises, we used deep sequencing of wild populations to reveal genetic va

222 However, unbiased deep-sequencing of edited reporters shows that all edito

223 MPORTANCE Here, we report the discovery, via deep sequencing, of numerous noncoding RNAs (ncRNAs) der

224 gut microbial profiling using 16S rRNA gene deep sequencing on 510 fecal specimens from 168 kidney t

225 0.01-0.15) driver indels missed by targeted deep-sequencing, outperforming the current best-practice

226 Through amplicon deep-sequencing placental malaria samples from women in

227 eveloped parallel RNA and DNA analysis after deep sequencing (PRDD-seq), which combines RNA analysis

228 Labs without the means to undertake deep sequencing projects can mine the data available to

229 ly identified via yeast display coupled with deep sequencing, providing a robust dataset for evaluati

230 Deep sequencing PSTVd genomes from plants inoculated wit

231 gene BCR-ABL1 Using bulk competitions with a deep-sequencing readout, we analyzed hundreds of mutatio

232 However, their characterization through deep sequencing remains challenging, in spite of rapid d

233 enhanced sensitivity, without necessitating deep sequencing, represent important steps toward realiz

234 Furthermore, our targeted deep sequencing results confirmed that our adeno-associa

235 e well documented, but no study has combined deep sequencing results from pulmonary and indoor microb

236 ion, the FoxO1 chromatin immunoprecipitation-deep sequencing results were aligned with those of pol I

237 Deep sequencing revealed a high-frequency variant in PRV

238 Deep sequencing revealed interpatient gp350 sequence var

239 Deep sequencing revealed similar mutational burdens and

240 m chromatin immunoprecipitation coupled with deep sequencing revealed that LXRalpha and LXRbeta occup

241 Deep sequencing revealed that metastatic tumours induced

242 Deep sequencing revealed that wild-type clones without m

243 icial linkages by primer extension, PCR, and deep sequencing reveals that a subtle interplay between

244 Ribo-seq, a technique for deep-sequencing ribosome-protected mRNA fragments, has e

245 RNA immunoprecipitation coupled with deep sequencing (RIP-seq) revealed that CDK12 regulates

246 RNA immunoprecipitation followed by deep sequencing (RIP-seq) reveals that cytoplasmic CBFB

247 aled by RNA immunoprecipitation coupled with deep sequencing (RIP-Seq); and that PUMPKIN can bind RNA

248 d poles by combining cell fractionation with deep-sequencing (Rloc-seq).

249 RNA deep sequencing (RNA-seq) has greatly facilitated the st

250 Deep sequencing (RNA-Seq) of RNA from undifferentiated a

251 RNA deep sequencing showed significant downregulation of gen

252 miRNA deep-sequencing showed that human and rodent parathyroid

253 in chromatin immunoprecipitation followed by deep sequencing signal intensity for SNPs within functio

254 (P = 0.007).Conclusions: In this integrative deep sequencing study of SERPINA1 with alpha-1 antitryps

255 assembly of short sequence reads produced by deep sequencing, such as de-novo assemblers, ignore the

256 With the rapid development of deep sequencing techniques in the recent years, enhancer

257 n performed at large scale and combined with deep sequencing techniques, CRISPR-based perturbations c

258 the litchi small RNA population with various deep-sequencing techniques and in-depth bioinformatic an

259 The advent of deep-sequencing techniques has revealed that mutations i

260 In the present study, we applied deep-sequencing techniques within a Bayesian hierarchica

261 The advent of deep sequencing technologies led to the identification o

262 Recent advances in deep-sequencing technologies and in silico prediction al

263 employed the next generation high-throughput deep sequencing technology to sequence all small RNAs an

264 Using deep-sequencing technology, we observed an increase in d

265 d lymphocyte reaction, T-cell receptor (TCR) deep sequencing, tetramer-guided naive CD4 T-cell precur

266 , and quantified particle biodistribution by deep sequencing the barcodes.

267 Combined with deep sequencing, this library was used to investigate th

268 used transposon insertional mutagenesis and deep sequencing (Tnseq) to investigate rifampicin-specif

269 cherichia coli using cell sorting coupled to deep sequencing to analyse large libraries designed on t

270 We used deep sequencing to analyze this diversity in chagasic pa

271 e used live-cell single molecule imaging and deep sequencing to assess determinants of Satb1 binding-

272 Here we use deep sequencing to characterize abundant mtDNA deletions

273 We use deep sequencing to define (i) transcriptomes of Brn3a- a

274 Here, we use deep sequencing to define the evolutionary trajectories

275 In conclusion, deep sequencing to determine the TH index could serve as

276 , we performed bulked segregant analysis and deep sequencing to fine map it to an approximately 1 Mb

277 ghput mutagenesis, functional screening, and deep sequencing to identify mutations from a total of 7,

278 We used precision nuclear run-on with deep sequencing to map and to quantify Pol II on the HSV

279 election for antibody binding and subsequent deep sequencing to map epitopes.

280 egmented amplification approach coupled with deep sequencing to profile DENV-3 intrahost diversity in

281 We developed an assay using Primer ID-tagged deep sequencing to quantify HIV-1 splicing.

282 ribosome affinity purification coupled with deep-sequencing (TRAP-seq) in Caenorhabditis elegans, we

283 Application of Ultra-Deep Sequencing (UDS) immensely increases the sensitivit

284 While deep sequencing uncovers rare tumor variants, the hetero

285 Deep sequencing viral populations at multiple time point

286 Deep sequencing was applied to blood plasma samples from

287 absence of the index malignant clone by IGH deep sequencing was associated with better EFS (P = .034

288 Deep sequencing was performed to analyze the biogenesis

289 Deep sequencing was performed to identify changes in the

290 In this study, deep sequencing was used to characterize prokaryotic vir

291 Using deep sequencing, we analyzed the immunoglobulin heavy ch

292 Using qPCR and long-read amplicon deep sequencing, we detected subpopulations with T1RMS r

293 Using chromatin immunoprecipitation and deep sequencing, we found that TLE3 occupies distal enha

294 Based on extensive LOXL1 deep sequencing, we report here the identification of a

295 eal-time polymerase chain reaction assay and deep sequencing, we reveal the presence of mansonellosis

296 Using deep sequencing, we show that ecDNA originates from the

297 toes, polymerase chain reaction and amplicon deep sequencing were used to track species and clone-spe

298 Weekly, skin swabs for microbiome analysis (deep sequencing) were taken, AD severity was assessed, a

299 Targeted deep sequencing/whole-exome sequencing was performed pre

300 per mL had drug resistance testing by ultra-deep sequencing, with drug resistance defined as interme