ライフサイエンスコーパス: dehalogenase

1 nly in the latter (Kcat = 0.03% of wild-type dehalogenase).

2 r a haloacetate dehalogenase or a haloalkane dehalogenase.

3 oyl-CoA more tightly than does the wild-type dehalogenase.

4 nt of dichloroethane catalyzed by haloalkane dehalogenase.

5 source of the catalytic power of haloalkane dehalogenase.

6 W137F mutant enzymes of 4-chlorobenzoyl-CoA dehalogenase.

7 -CoA undergoes major changes upon binding to dehalogenase.

8 er enzyme of halocarbon metabolism, haloacid dehalogenase.

9 ized product in the active site of wild-type dehalogenase.

10 on, and near 1610 cm-1 in the active site of dehalogenase.

11 to the active site of 4-(chlorobenzoyl)-CoA dehalogenase.

12 ectroscopy in its free form and bound to the dehalogenase.

13 fluorination activity to a nondefluorinating dehalogenase.

14 esence of a gene that putatively codes for a dehalogenase.

15 new subtype within the alpha/beta hydrolase dehalogenases.

16 measured for wild-type and Glu232Asp mutant dehalogenases.

17 erial, isomer-selective 3-chloroacrylic acid dehalogenases.

18 alamin-dependent methyltransferases, and the dehalogenases.

19 nover rates in the wild-type and H90Q mutant dehalogenases.

20 er-containing, membrane-associated reductive dehalogenases.

21 genases as well as metabolic and cometabolic dehalogenases.

22 xpression levels of their multiple reductive dehalogenases.

23 dration of functionally important regions of dehalogenases.

24 six rRNA operons and six predicted reductive dehalogenases.

25 In wild-type dehalogenase, 22% of the bound substrate accumulated as

26 The recent crystal structure of the dehalogenase-4-HBA-CoA complex reveals two hydrogen bond

27 sis that MSAD and trans-3-chloroacrylic acid dehalogenase, a tautomerase superfamily member preceding

28 ndent subfamilies, we propose that reductive dehalogenases achieve reduction of the organohalide subs

29 ay have evolved convergently from homologous dehalogenases across phyla (Cnidaria, Echinodermata, and

30 In addition to the ligase and reductive dehalogenase activities, hydrolytic 4-chlorobenzoyl-CoA

31 ables the acquisition of a basal iodomethane dehalogenase activity as yet unknown in natural alkane m

32 amin B(12) as a model reactant for reductive dehalogenase activity.

33 rystal structure of the complex of 4-CBA-CoA dehalogenase and 4-HBA-CoA we propose that aspartate 145

34 sive similarity to the ancient L-2-halo-acid dehalogenase and DDDD phosphohydrolase superfamilies, bu

35 cus rhodochrous NCIMB 13064, and haloalcohol dehalogenase and epoxide hydrolase from Agrobacterium ra

36 oethane by a carboxylate group in haloalkane dehalogenase and in water.

37 l protein Anc(HLD-RLuc) which catalyses both dehalogenase and luciferase reactions.

38 this domain is similar to that of haloalkane dehalogenase and shares the alpha/beta hydrolase fold.

39 work investigates complexes formed by D145N dehalogenase and substrate or product.

40 The k(obs) for EAr formation in wild-type dehalogenase and the more active dehalogenase mutants (Y

41 e activities, hydrolytic 4-chlorobenzoyl-CoA dehalogenase and thioesterase activities, 4-hydroxybenzo

42 tion of a soluble, oxygen-tolerant reductive dehalogenase and, by combining structure determination w

43 current knowledge about catabolic reductive dehalogenases and the electron transfer chain they are p

44 hlorobenzoyl-CoA ligase, 4-chlorobenzoyl-CoA dehalogenase, and 4-hydroxybenzoyl-CoA thioesterase.

45 species contains three enzymes: a ligase, a dehalogenase, and a thioesterase.

46 , glutathione transferase, haloalkanoic acid dehalogenase, and isoprenoid synthase), with five superf

47 ed Protein A, three wild-types of haloalkane dehalogenases, and a mutant stabilized by protein engine

48 s phosphoesterases, ATPases, phosphonatases, dehalogenases, and sugar phosphomutases acting on a rema

49 ts for EMc formation in wild-type and mutant dehalogenase are approximately 200 s(-1) while the rate

50 (or bvcA) encoding reductive vinyl chloride dehalogenases are important to achieve complete dechlori

51 e evolutionarily related catabolic reductive dehalogenases are oxygen tolerant, with those that are n

52 Reductive dehalogenases are responsible for biological dehalogenat

53 nd 11 microM(-1) x s(-1)) binding to the two dehalogenases are similar in value.

54 n, it is likely that tetrachlorohydroquinone dehalogenase arose from a maleylacetoacetate isomerase.

55 he structurally modified 4-chlorobenzoyl-CoA dehalogenase at kcat = 0.06 s-1 and Km = 50 microM.

56 ase structure with the 4-(chlorobenzoyl)-CoA dehalogenase backbone.

57 Subsequently, glutathione-dependent dehalogenases (BbdI and BbdE) catalyze the thiolytic rem

58 TCHQ dehalogenase binds the glutathione involved in the thiol

59 evel cis-CaaD and trans-3-chloroacrylic acid dehalogenase (CaaD) activities, with the cis-CaaD activi

60 The enzymes trans-3-chloroacrylic acid dehalogenase (CaaD) and cis-3-chloroacrylic acid dehalog

61 reviously studied trans-3-chloroacrylic acid dehalogenase (CaaD) and with other members of the 4-oxal

62 merase (4-OT) and trans-3-chloroacrylic acid dehalogenase (CaaD) are members of the tautomerase super

63 trans-3-Chloroacrylic acid dehalogenase (CaaD) converts trans-3-chloroacrylic acid

64 e active site of the enzyme 3-chloroacrylate dehalogenase (CaaD), isolated from a pseudomonad growing

65 ichloropropene is trans-3-chloroacrylic acid dehalogenase (CaaD), which converts the trans-isomers of

66 critical for the trans-3-chloroacrylic acid dehalogenase (CaaD)-catalyzed conversion of trans-3-halo

67 the heterohexamer trans-3-chloroacrylic acid dehalogenase (CaaD).

68 ion with an enrichment of vcrA (VC reductive dehalogenase)-carrying Dehalococcoides, whereas ethene p

69 the adjacent subunit, to 4-chlorobenzoyl-CoA dehalogenase catalysis.

70 examines the role of binding interactions in dehalogenase catalysis.

71 entified aspartate 145 as being essential to dehalogenase catalysis.

72 Isomer-specific 3-chloroacrylic acid dehalogenases catalyze the hydrolytic dehalogenation of

73 In this paper, we report the kinetics for dehalogenase-catalyzed 4-fluorobenzoyl-CoA (4-FBA-CoA) a

74 m of tyrosine, while tetrachlorohydroquinone dehalogenase catalyzes a more specialized reaction, it i

75 Tetrachlorohydroquinone (TCHQ) dehalogenase catalyzes the conversion of TCHQ to 2,6-dic

76 t bound to the active site of asparagine 145 dehalogenase catalyzes the deamidation of the asparagine

77 4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolysis of 4-CBA-CoA to 4-

78 4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolysis of 4-CBA-CoA to 4-

79 The enzyme 4-chlorobenzoate-CoA dehalogenase catalyzes the hydrolysis of 4-chlorobenzoat

80 4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolytic dehalogenation of

81 4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolytic dehalogenation of

82 4-chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolytic dehalogenation of

83 4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolytic dehalogenation of

84 Furthermore, tetrachlorohydroquinone dehalogenase catalyzes the isomerization of maleylaceton

85 Tetrachlorohydroquinone dehalogenase catalyzes the reductive dehalogenation of t

86 Tetrachlorohydroquinone dehalogenase catalyzes the reductive dehalogenation of t

87 Tetrachlorohydroquinone dehalogenase catalyzes the reductive dehalogenation of t

88 Tetrachlorohydroquinone dehalogenase catalyzes the replacement of chlorine atoms

89 Tetrachlorohydroquinone dehalogenase catalyzes two successive reductive dehaloge

90 brominated benzenes, including the reductive dehalogenases CbdbA80 and CbrA.

91 he X-ray crystal structure of chlorothalonil dehalogenase (Chd) from Pseudomonas sp. CTN-3, with 15 o

92 e inhibitors of the cis-3-chloroacrylic acid dehalogenase ( cis-CaaD) homologue Cg10062 found in Cory

93 cis-3-Chloroacrylic acid dehalogenase (cis-CaaD) catalyzes the hydrolytic dehalog

94 e gene encoding the cis-3-chloroacrylic acid dehalogenase (cis-CaaD) from coryneform bacterium strain

95 cis-3-Chloroacrylic acid dehalogenase (cis-CaaD) from Pseudomonas pavonaceae 170

96 inetic mechanism of cis-3-chloroacrylic acid dehalogenase (cis-CaaD) has now been examined using stop

97 logenase (CaaD) and cis-3-chloroacrylic acid dehalogenase (cis-CaaD) represent the two major classes

98 nd expression of the bacterial chloromethane dehalogenase cmuA gene in the A. thaliana phyllosphere c

99 describe the development of an alkyl halide dehalogenase-compatible chloroalkane linker phosphoramid

100 in the structure of the 4-HBA-CoA-wild-type dehalogenase complex.

101 X-ray crystallographic analysis of H90Q dehalogenase complexed with 4-HBA-CoA revealed that the

102 ain normal (nonresonance) Raman data for the dehalogenase complexes in the 100-300 microM range and h

103 ntly described corrinoid-dependent reductive dehalogenases, constitute a new subclass within the nitr

104 In this process, a reductive dehalogenase (CprA), couples the oxidation of an electro

105 One of the reductive dehalogenases, CprA, is encoded by a well-characterized

106 ecord kinetic time courses of the haloalkane dehalogenase DbjA and analyzed 150 combinations of enzym

107 m extorquens DM4 expresses a dichloromethane dehalogenase (DcmA) that has been acquired through horiz

108 e [Formula: see text]-knotted alpha-haloacid dehalogenase (DehI) protein, we introduce a topological

109 The dehalogenase derives its catalytic power from: (i) bring

110 rmostability of the model enzymes haloalkane dehalogenase DhaA and gamma-hexachlorocyclohexane dehydr

111 For comparison, we also mapped haloalkane dehalogenases DhaA and LinB, both of which contain signi

112 The catalytic site of haloalkane dehalogenase DhlA is buried more than 10 A from the prot

113 considering the S(N)2 reaction of haloalkan dehalogenase (DhlA), analyze the energetics and dynamics

114 we take as a test case the enzyme haloalkane dehalogenase (DhlA), with a 1,2-dichloroethane substrate

115 domains located in the lumen and a haloacid-dehalogenase domain exposed to the chloroplast stroma.

116 found that the conserved C-terminal haloacid dehalogenase domain of EYA1, which has been reported to

117 ently expressed of four detectable reductive dehalogenases during 1,2-dichloroethane exposure, sugges

118 rain 195 showed genes encoding for reductive dehalogenases (e.g., tceA) were not affected during the

119 gen-halide lyases (EC 4.5.1), and haloalkane dehalogenases (EC 3.8.1).

120 attention and development: halalkanoic acid dehalogenases (EC 3.8.1.2), hydrogen-halide lyases (EC 4

121 as putida strain PP3 produces two hydrolytic dehalogenases encoded by dehI and dehII, which are membe

122 Deletion or suppression of the dehalogenase-encoding condet (cdt) gene in Drosophila al

123 by the horizontal transfer of key reductive dehalogenase-encoding genes.

124 We apply our method, GASPS, to the haloacid dehalogenase, enolase, amidohydrolase and crotonase supe

125 f the pseudouridine glycosidase and haloacid dehalogenase enzyme families, respectively, catalyze C-r

126 investigate ligand passage in the haloalkane dehalogenase enzyme LinB and the effect of replacing leu

127 hosphoglucomutase (beta-PGM) of the haloacid dehalogenase enzyme superfamily (HADSF) catalyzes the co

128 e large enzyme family, the haloalkanoic acid dehalogenase enzyme superfamily (HADSF) form a "mold" in

129 The haloacid dehalogenase enzyme superfamily (HADSF) is largely compo

130 phatase branch of the HAD (haloalkanoic acid dehalogenase) enzyme superfamily, yet it has evolved to

131 lden inversion mechanism of the fluoracetate dehalogenase FAcD (RPA1163) has been studied by subjecti

132 bacterial homodimeric enzyme, fluoroacetate dehalogenase (FAcD).

133 ain, which has high homology to the haloacid dehalogenase family of phosphatases, has not been defini

134 phosphatase domain belonging to the haloacid dehalogenase family of phosphatases.

135 g of eight model enzymes from the haloalkane dehalogenase family.

136 isms of nucleotidase members of the haloacid dehalogenase family.

137 or a member of the Mg(2+)-dependent haloacid dehalogenase family.

138 ponds to At5g32470, encoding a HAD (haloacid dehalogenase) family phosphatase fused to a TenA (thiami

139 chitecture, a fusion of a predicted haloacid dehalogenase fold with a previously unidentified GCN5-re

140 Reductive dehalogenases form a distinct subfamily of cobalamin (B1

141 le in the active site of 4-chlorobenzoyl-CoA dehalogenase, forming a transient covalent link at the 4

142 taining 3-chloro-4-hydroxybenzoate reductive dehalogenase from Desulfitobacterium chlororespirans.

143 glutathione (GSH)-dependent dichloromethane dehalogenase from Methylophilus sp. strain DM11 catalyze

144 Zn(II)-dependent, chlorothalonil-hydrolyzing dehalogenase from Pseudomonas sp. CTN-3 (Chd), enabling

145 dimensional structure of 4-chlorobenzoyl-CoA dehalogenase from Pseudomonas sp. strain CBS-3.

146 ferent microorganisms: engineered haloalkane dehalogenase from Rhodococcus rhodochrous NCIMB 13064, a

147 show that LinB, an HCH-converting haloalkane dehalogenase from Sphingobium indicum B90A, is also able

148 The S(N)2 reaction of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10, which

149 by Asp-124 in the active site of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10.

150 nd product inhibition patterns of haloalkane dehalogenases from Xanthobacter autotrophicus GJ10 (XaDH

151 Diversification of reductive dehalogenase functions appears to have been mediated by

152 undwater among the samples with VC reductive dehalogenase gene (bvcA and vcrA) abundances reaching 10

153 gene, distantly related to the chlorophenol dehalogenase gene cprA (pairwise amino acid identity: 23

154 A quantitative PCR (qPCR) assay for the dehalogenase gene dhlA was developed to monitor DCA-degr

155 s by quantifying major orthologous reductive dehalogenase gene groups.

156 ular analysis targeting a putative reductive dehalogenase gene of D. chlorocoercia or the bphA gene o

157 ic link between transposition of ISPpu12 and dehalogenase gene silencing.

158 A novel reductive dehalogenase gene, distantly related to the chlorophenol

159 ional genes (etnC and etnE) and VC reductive dehalogenase genes (bvcA and vcrA) were strongly related

160 A pair of dehalogenase genes from Xanthobacter autotrophicus was e

161 Examination of reductive dehalogenase genes implicated in ethene formation reveal

162 enases were the most abundant halogenase and dehalogenase genes, respectively.

163 f substrate to product and of D145N to D145D dehalogenase go on simultaneously.

164 5 were found to contain a conserved haloacid dehalogenase (HAD) domain, which was found to be require

165 The haloalkanoic acid dehalogenase (HAD) enzyme superfamily is the largest fam

166 se (HisB) subfamily of the haloalkanoic acid dehalogenase (HAD) enzyme superfamily.

167 Haloacid dehalogenase (HAD) family phosphatases are widespread in

168 The 2-haloalkanoic acid dehalogenase (HAD) family, which contains both carbon an

169 e phosphoserine phosphatases of the haloacid dehalogenase (HAD) family.

170 A gene cluster containing an (S)-2-haloacid dehalogenase (had) gene was up-regulated in cells grown

171 o proteins containing an N-terminal haloacid dehalogenase (HAD) phosphatase domain followed by four p

172 Mammalian phosphatases of the haloacid dehalogenase (HAD) superfamily have emerged as important

173 The haloacid dehalogenase (HAD) superfamily includes a variety of enz

174 The haloacid dehalogenase (HAD) superfamily is comprised of structura

175 to the phosphatase subgroup of the haloacid dehalogenase (HAD) superfamily, and propose a function f

176 zed protein NT5DC2, a member of the haloacid dehalogenase (HAD) superfamily, as previously unrecogniz

177 In phosphatases and mutases of the haloacid dehalogenase (HAD) superfamily, phosphoaspartate serves

178 pFHy1 (At1g79790), belonging to the haloacid dehalogenase (HAD) superfamily, seven candidates for the

179 sEH N-terminal fold belongs to the haloacid dehalogenase (HAD) superfamily, which comprises a vast m

180 P-1 suggested a relationship to the haloacid dehalogenase (HAD) superfamily, which contains a number

181 beta-PGM is a member of the haloacid dehalogenase (HAD) superfamily, which includes the sarco

182 endent, suggesting an enzyme of the haloacid dehalogenase (HAD) superfamily.

183 The haloacid dehalogenase (HAD)-like enzymes comprise a large superfa

184 -regulated, novel low-Pi-responsive haloacid dehalogenase (HAD)-like hydrolase, OsHAD1 While OsHAD1 i

185 as PfHAD1), encoding a homologue of haloacid dehalogenase (HAD)-like sugar phosphatases.

186 Mammalian haloacid dehalogenase (HAD)-type phosphatases are an emerging fam

187 Another gene encoded a homolog of 2-haloacid dehalogenase (HAD).

188 dingly, the atomic structure of L-2 haloacid dehalogenase has been fitted into the relevant domain of

189 oA and 4-FBA-CoA bound to WT and H90Q mutant dehalogenase have broad features near 1500 and 1220 cm(-

190 alcohol dehydrogenase (AdhS) and halohydrin dehalogenase (HHDH) was investigated as a model bioelect

191 mined that JNA harbors at least 19 reductive dehalogenase homologous (rdh) genes including orthologs

192 ome of strain GEO12 revealed seven reductive dehalogenase homologous (rdh) genes, including tceA and

193 d that two proteins encoded by the reductive dehalogenase homologous genes CbdbA1092 and CbdbA1503 we

194 8-P phosphatase is a member of the haloacid dehalogenase hydrolase superfamily.

195 The enzyme 4-chlorobenzoyl-CoA dehalogenase hydrolyzes 4-chlorobenzoyl-CoA (4-CBA-CoA)

196 sistent with deiodination of labeled knob by dehalogenases in hepatocyte microsomes and uptake of the

197 d to determine the activity of the reductive dehalogenases in resting cell assays of strain CBDB1 wit

198 TCHQ dehalogenase, in contrast to most members of the superfa

199 ble of switching the catalytic activity of a dehalogenase into a nitroreductase.

200 constant for hydrolysis of EAr in wild-type dehalogenase is 20 s(-1) and in the H90Q mutant, 0.13 s(

201 TCHQ dehalogenase is a member of the glutathione S-transferas

202 Tetrachlorohydroquinone dehalogenase is a member of the glutathione S-transferas

203 Tetrachlorohydroquinone dehalogenase is found in Sphingomonas chlorophenolica, a

204 benzoyl-CoA catalyzed by 4-chlorobenzoyl-CoA dehalogenase is investigated using combined QM/MM approa

205 Tetrachlorohydroquinone (TCHQ) dehalogenase is profoundly inhibited by its aromatic sub

206 TCHQ dehalogenase is severely inhibited by its aromatic subst

207 larizing forces and reactivity seen here for dehalogenase is similar to that reported for the additio

208 ctive site region of tetrachlorohydroquinone dehalogenase is very similar to those of the correspondi

209 the only heme-containing peroxide-dependent dehalogenase known to be capable of removing halogens in

210 residues 414-436), which flanks the haloacid dehalogenase-like catalytic domain (residues 251-413), c

211 d region of Pah1, in particular the haloacid dehalogenase-like domain containing the DIDGT catalytic

212 eas some residues interact with the haloacid dehalogenase-like domain.

213 The conserved N-LIP and haloacid dehalogenase-like domains of Pah1 are required for phosp

214 EC 3.1.3.3) and belongs to the l-2-haloacid dehalogenase-like protein superfamily.

215 tein lipin 1 and the superfamily of haloacid dehalogenase-like proteins.

216 lorinases (LinA1 and LinA2) and a haloalkane dehalogenase (LinB) from Sphingobium indicum B90A.

217 This haloalkane dehalogenase lowers the activation barrier for dehalogen

218 spectra of the complexes involving the D145A dehalogenase mutant that are unable to form an EMc.

219 n wild-type dehalogenase and the more active dehalogenase mutants (Y65D and A112V) was taken to be an

220 talysis in the F64P, G113A, G113S, and G113N dehalogenase mutants was very slow with k(cat) values ra

221 Catalysis in the Y65D and A112V dehalogenase mutants were almost as efficient as catalys

222 Genes encoding 17 putative reductive dehalogenases, nearly all of which were adjacent to gene

223 chlorine is then removed hydrolytically by a dehalogenase of the alpha/beta hydrolase superfamily (Bb

224 observed in phosphonatase and the 2-haloacid dehalogenase of the HAD enzyme superfamily indicated com

225 the active site aspartate in the haloalkane dehalogenase of Xanthobacter autothropicus have been com

226 of Asp-124 at the active site of haloalkane dehalogenase of Xanthobacter autothropicus.

227 Covalent immobilization of haloalkane dehalogenase on a surface support displaying poly(sorbit

228 not exhibit activity as either a haloacetate dehalogenase or a haloalkane dehalogenase.

229 yme-specific rate constants (k(cat)) for the dehalogenases PceA and TceA: 400 and 22 substrate molecu

230 ion catalysis by tetrachloroethene reductive dehalogenase (PceA) of Sulfurospirillum multivorans was

231 hafniesne Y51), which only has one reductive dehalogenase (PceA).

232 operties and the tetrachloroethene reductive dehalogenase (PceA-RDase) localization did not result in

233 tetrachloro-p-hydroquinone (TeCH) reductive dehalogenase (PcpC), which is a glutathione (GSH) S-tran

234 EYA1, a haloacid dehalogenase phosphatase and transcription factor, is cr

235 amino acid residues at these stations by the dehalogenases, phosphonatases, phosphatases, and phospho

236 Reductive dehalogenases play a critical role in the microbial deto

237 tic strategy and the active site of haloacid dehalogenase proteins shares a common geometry and ident

238 nantly mediated by a single, novel reductive dehalogenase (RDase) catalyzing chlorine removal from bo

239 acterial strains expressing active reductive dehalogenase (RDase) enzymes play key roles in the trans

240 vealed the presence of 29 putative reductive dehalogenase (RDase) genes.

241 ide-respiring bacteria that harbor reductive dehalogenases (RDases) capable of dehalogenating these p

242 Idiosyncratic combinations of reductive dehalogenase (rdh) genes are a distinguishing genomic fe

243 ology with the cobalamin-dependent reductive dehalogenase (RdhA), however the role played by cobalami

244 containing bacteria with different reductive dehalogenases (rdhA) genes can lead to variable dual C-C

245 dies have identified a critical role for the dehalogenase residue Asp 145 in close proximity to the l

246 The crystallographic investigation of this dehalogenase revealed that the enzyme is a trimer.

247 otic members of the cis-3-chloroacrylic acid dehalogenase subgroup are limited to fungal species, whe

248 The phosphotransferases of the haloalkanoate dehalogenase superfamily (HADSF) act upon a wide range o

249 Alpha-PMM1, like most haloalkanoic acid dehalogenase superfamily (HADSF) members, consists of tw

250 tional space of the ubiquitous haloalkanoate dehalogenase superfamily (HADSF) was revealed by screeni

251 The protein, a member of the haloalkanoate dehalogenase superfamily (subfamily IIB), was purified t

252 cus on the phosphatases of the haloalkanoate dehalogenase superfamily and the thioesterases of the ho

253 hosphatases that are members of the haloacid dehalogenase superfamily contains the catalytic motif DX

254 In haloacid dehalogenase superfamily enzymes, substrate specificity

255 tion assignment of the unknown haloalkanoate dehalogenase superfamily member BT2127 (Uniprot accessio

256 with molecular structures of other haloacid dehalogenase superfamily members that were crystallized

257 terminal domain that belongs to the haloacid dehalogenase superfamily of enzymes.

258 Tpp1 shows similarity to the l-2-haloacid dehalogenase superfamily of enzymes.

259 The haloalkanoic dehalogenase superfamily serves as an excellent model sy

260 rotein-tyrosine phosphatases of the haloacid dehalogenase superfamily, as being responsible for depho

261 known and a few as belonging to the haloacid dehalogenase superfamily, but has no known biological fu

262 KdsC belongs to the broad haloacid dehalogenase superfamily.

263 th sides that is reminiscent of the haloacid dehalogenase superfamily.

264 different functional classes of the haloacid dehalogenase superfamily.

265 which are members of the conserved haloacid dehalogenase superfamily.

266 horylated by AlnB, an enzyme of the haloacid dehalogenase superfamily.

267 The HAD (haloacid dehalogenase) superfamily includes phosphoesterases, ATP

268 The dehalogenase TceA and the Ni-Fe hydrogenase HupL transcr

269 ry revealed that a trichloroethene reductive dehalogenase (TceA) homologue was the most consistently

270 rst structure of a Zn(II)-dependent aromatic dehalogenase that does not require a coenzyme.

271 id approach to evolve a bacterial halohydrin dehalogenase that improves the volumetric productivity o

272 ered a mechanism for tetrachlorohydroquinone dehalogenase that involves a nucleophilic aromatic subst

273 on its degradation by the enzyme haloalkane dehalogenase that is accompanied by a change of local pH

274 Drosophila express a native flavin-dependent dehalogenase that is homologous to the enzyme responsibl

275 -dependent and quinone-independent reductive dehalogenases that are distinguishable at the amino acid

276 d investigations of the haloalkene reductive dehalogenases that catalyze similar reactions.

277 er, distinguishes CaaD from those hydrolytic dehalogenases that form alkyl-enzyme intermediates.

278 substrate and inhibitor specificity for the dehalogenase, the enzyme was found to require an hydroxy

279 sis that MSAD and trans-3-chloroacrylic acid dehalogenase, the preceding enzyme in the trans-1,3-dich

280 For wild-type dehalogenase, the rate constant for a single turnover is

281 The reaction mechanism of the dehalogenases, the most recently discovered class of B12

282 For H90Q dehalogenase, these rate constants are 1.6 x 10(-2) and

283 resence of genes encoding putative reductive dehalogenases throughout the phylum expanded the phyloge

284 to unmask the intrinsic KIE of the reductive dehalogenase (TmrA) suggesting that enzyme binding and/o

285 t the rate of the S(N)2 step of a haloalkane dehalogenase using a generative maximum-entropy (MaxEnt)

286 r to facilitate electrophilic catalysis, the dehalogenase utilizes a strong polarizing interaction be

287 ode a corrinoid-containing 1,2-DCP reductive dehalogenase was detected.

288 mulation of arylated enzyme in the wild-type dehalogenase was not observed in the mutant.

289 tive expression of Dehalococcoides-reductive dehalogenases was observed.

290 ratase/isomerase family, 4-chlorobenzoyl-CoA dehalogenase, was altered by site-directed mutagenesis t

291 absent in the wild-type 4-chlorobenzoyl-CoA dehalogenase, was shown to occur with the structurally m

292 the two proteins myoglobin and fluoroacetate dehalogenase, we present a systematic comparison of crys

293 e strong evidence that four to six reductive dehalogenases were involved in the dehalogenation of all

294 e, no-metal chloroperoxidases and haloalkane dehalogenases were the most abundant halogenase and deha

295 With the exception of haloalkane dehalogenase, which binds very small substrates in a nar

296 thereby distinguishing CaaD from a number of dehalogenases whose mechanisms proceed through an alkyl-

297 Treatment of the dehalogenase with diethyl pyrocarbonate resulted in comp

298 lmost as efficient as catalysis in wild-type dehalogenase with k(cat) values of 0.1-0.6 s(-1).

299 Chemical modification of the dehalogenase with N-bromosuccinimide resulted in full lo

300 adaptation must have selected for reductive dehalogenases with different mechanistic motifs.