ライフサイエンスコーパス: denature

1 s are able to form haze alone when thermally denatured.

2 sorbed to the air-water interface are partly denatured.

3 tic pressure inside the NMR sample cell from denaturing (2.5 kbar) to native (1 bar) conditions.

4 Denatured AChE was eluted with 1% trifluoroacetic acid.

5 e adapted and used a highly stringent Tandem Denaturing Affinity Purification (TDAP) method, coupled

6 3 activity on full-length VWF arises without denaturing agent as evidenced by FCS and gel-based multi

7 Exposing the intracellular domain to a denaturing agent increases the energy required to open t

8 proaches studying the effect of proteases or denaturing agents on the physicochemical properties of P

9 les to elevated temperature, proteolytic and denaturing agents, and extend the operable pH range for

10 from its structured state in the absence of denaturing agents.

11 The trypsin-hydrolyzed denatured albumin exhibited the highest antioxidant acti

12 2E8, while DDM was additionally added to SDS-denatured alphaLA and betaLG.

13 ctionally; emerging unfolded polypeptide can denature an already-folded domain.

14 fied with cow protein isolates that had been denatured and glycated by thermal treatment.

15 s of proteoforms and their complexes in both denatured and native modes of operation, revealing infor

16 s a sufficiently high barrier separating the denatured and native states that folding appears coopera

17 y populated on parallel pathways between the denatured and native states.

18 In both denatured and nondenatured samples, this epitope can be

19 fragments and genomic DNA samples were first denatured and then annealed in the presence of blocking

20 SDCPI was the least denatured and VDCPI the most denatured, demonstrating th

21 The AS sodium dodecyl sulfate (SDS) denatures and unfolds globular proteins under most condi

22 the ability to run the samples in different denaturing and nondenaturing buffer allows defining true

23 nformation, binds to a variety of deposited, denatured, and aggregated proteins, in addition to bindi

24 peanut, and pistachio flours using three non-denaturing aqueous (phosphate, borate, and carbonate) bu

25 single and interactive effects of three non-denaturing aqueous (phosphate, borate, and carbonate) bu

26 Overall, harsh conditionsare required to denature Ara h 6 and to significantly reduce its IgE-bin

27 igomers in vitro, but only the rice isoforms denature at nonpermissive temperatures.

28 Collagen will denature at relatively low temperatures, such as during

29 tructure at high temperatures, Trp-cage cold denatures at 210 K into a compact, partially folded stat

30 elatively unstable three-helical bundle that denatures at pH <6.2 due to protonation of key histidine

31 nstrate the relative stability of RNA in the denaturing/basic IEX mobile phase, lay out a protocol to

32 ation (62.5 degrees C, 30 min) of human milk denatures beneficial proteins.

33 with a sigmoidal increase in intermolecular (denatured) beta-sheets at a TOD (FTIR) of 72.4 degrees C

34 cocyanin and c-phycocyanin) disassembled and denatured between 50 and 70 degrees C.

35 h folded, intrinsically disordered, and urea-denatured biomolecules.

36 Non-denaturing bisulfite treatment modifies the cytosines on

37 tein binding in the presence of NaCl and for denatured BSA/lutein binding.

38 ble immobilization (SPRI) beads with protein-denaturing buffers, and then identified by proteomics.

39 aggregates that contain amyloid fibers when denatured, but there is little evidence that ex vivo cat

40 nfolded by heating ( T(m) ~ 75 degrees C) or denatured by 1.5 M guanidine hydrochloride, the Ico8 cag

41 ding allow the chaperone to bind to proteins denatured by low pH, thereby protecting them from aggreg

42 Proteins were denatured by sodium dodecyl-sulphate (SDS) and precipita

43 We find that MoS2 exhibits robust denaturing capability to HP35, with its secondary struct

44 r trypsin followed by chymotrypsin to digest denatured cervid PrP, 19 peptides suitable for multiple

45 nstrated as a powerful vehicle for targeting denatured collagen (dColl) produced by disease or injury

46 whereas Fn is unusual in binding strongly to denatured collagen and more weakly to native collagen.

47 phonuclear cells (PMNs) ex vivo to adhere to denatured collagen in serum and polymyxin B independent

48 Severe collagen damage such as denatured collagen was also significantly less in the ir

49 ding sequence led to decreased Fn binding to denatured collagen.

50 cus on targeting native collagens as well as denatured collagens.

51 t from studying enzyme conformations under a denaturing condition, it is highly informative to manipu

52 sed conformational space sampling under urea-denaturing condition.

53 mature hydrolysis and low compatibility with denatured conditions.

54 ta2m (hbeta2m) forms amyloid-like fibrils in denaturing conditions (e.g. pH2.5) in the absence of NaC

55 ion auxiliaries that are removed under harsh denaturing conditions and have limited applicability.

56 We find that even under strongly denaturing conditions and in the absence of residual sec

57 study suggests that protein losses under non-denaturing conditions are more sensitive to protein stru

58 In addition, under non-denaturing conditions AV-1980R/A sera preferentially rec

59 ization method was developed using native or denaturing conditions by direct infusion into an Exactiv

60 Deriving equations under denaturing conditions has several pitfalls, and here, we

61 re the behavior of proteins under native and denaturing conditions in the presence of NaCl, CsCl, and

62 tional method to an IgG1 antibody under mild denaturing conditions indicates that the unfolding of ea

63 quently, affinity purification is used under denaturing conditions to capture proteins conjugated wit

64 Comparing non-denaturing conditions to FDF-PAGE uncovered extensive se

65 Because of the denaturing conditions used, this method ultimately leads

66 or which the SETA reactivity increased under denaturing conditions were found to engage in tertiary p

67 However, under mildly denaturing conditions, a ligand concentration-dependent

68 cally disordered proteins, or proteins under denaturing conditions, and studies of amyloid aggregate

69 itor oxidation in full-length proteins under denaturing conditions, as demonstrated on two biotherape

70 Under mildly denaturing conditions, CysC forms inactive domain-swappe

71 ssessed by immunoblotting under reducing and denaturing conditions, deconjugation-resistant LC3B accu

72 Under strongly denaturing conditions, folding was found to follow a con

73 Since solid-phase purification under protein-denaturing conditions, rather than sequence-specific nuc

74 Using denaturing conditions, the hemoglobin (Hb) tetramer diss

75 microscopy and biochemical assays under non-denaturing conditions.

76 nd was recovered only after extraction under denaturing conditions.

77 as required cofactors and/or unphysiological denaturing conditions.

78 tion), which are not detected under standard denaturing conditions.

79 pe VWF in the absence of shear but partially denaturing conditions.

80 their activities under thermal and chemical denaturing conditions.

81 in the fully folded state except under harsh denaturing conditions.

82 e precipitation of the modified strand under denaturing conditions.

83 e unfolding pathways under the two different denaturing conditions.

84 fewer periplasmic chaperones than their urea-denatured counterparts.

85 By contrast, denatured crystallins in age-related cataracts are bound

86 and ATP, the more expanded and unstructured denatured cyclophilin A is not encapsulated but is expel

87 Similar mixing experiments with acid-denatured cytochrome c and the resulting folding during

88 ein isolate was prepared (CPI) and thermally denatured (DCPI).

89 I was the least denatured and VDCPI the most denatured, demonstrating the poorest solubility and foam

90 tissues, even without a target; therefore, a denaturing detergent buffer is necessary for exhaustive

91 e to disruption of protein immobilization by denaturing detergents (SDS) and incubation at elevated t

92 After extraction with non-denaturing detergents, we affinity-purified 785 endogeno

93 ators across various bottom-up assays (i.e., denaturing/digestion conditions).

94 Heat-denatured DMBT1 lost capacity to inhibit twitching motil

95 ne whereas in situ specific detection of the denatured DNA by the PEPS occurred in the lower-temperat

96 DCPs prevent reassociation of denatured DNA strands: they make one of the two strands

97 melting point depressants capable of rapidly denaturing double stranded DNA (dsDNA) linkers, and 8 co

98 developed in the past decades, all involving denaturing dsDNA and hybridizing fluorescent nucleic aci

99 cheese protein (WP) components that had been denatured during cooking of the whey.

100 in marine organisms against the detrimental denaturing effects of another naturally occurring osmoly

101 he Tau protein, that TMAO can counteract the denaturing effects of urea by inhibiting protein-urea pr

102 platform is compatible with both native and denaturing electrospray, operates with a throughput of u

103 de the suitable electrostatic charge and non-denaturing environment for enzyme immobilization, but it

104 These denaturing environments are thought to be good solvents

105 Therefore, typical denaturing environments cannot be classified as good sol

106 effects lead to conformational statistics in denaturing environments that are congruent with those of

107 then do generic protein sequences expand in denaturing environments?

108 AD cofactor, in the case of AAOx and AOx, or denatured enzyme in the case of PyOx and ChOx.

109 ic species (either reaction side products or denatured enzymes, or both) on the cellulosic substrate

110 In addition, dsRNA is difficult to denature even at elevated temperature, and the extinctio

111 The native and denaturing extraction protocols have captured 1109 orang

112 Treatment of SMVs with urea denatured extramembrane components of complex V, elimina

113 cAMP binding stabilizes the CBD-A against a denaturing force, and increases the folded state lifetim

114 erapeutic efficacy of a treatment based on a denatured form of the major allergen, phospholipase A2,

115 adhesin binds E. coli B LPS in its native or denatured form.

116 d probe only modified active hNE and not its denatured form.

117 this problem, we established FDF-PAGE (fully-denaturing formaldehyde polyacrylamide gel electrophores

118 ng to a random distribution of unstructured, denatured forms, the results provide evidence for new co

119 Incorporation of extensively heated (heat-denatured) forms of milk and egg into the diets of child

120 and binding is assessed by measuring ligands denatured from the complexes.

121 ad of oxidative DNA cleavage is confirmed by denaturing gel electrophoresis and surface group pKa mea

122 variants within the gene were determined by denaturing gel electrophoresis or automated capillary-ba

123 l proteins were extracted and resolved using denaturing gel electrophoresis.

124 fy the functional form of TBCD and using non-denaturing gels and immunoblotting, we analyzed lysates

125 sured for individual tRNA species using acid denaturing gels, or comparatively at the genome level us

126 ow charging levels were validated using acid denaturing gels.

127 on directly in just 100 ng of non-fragmented denatured genomic DNA extracted from tomato seeds.

128 rea-denatured lactate dehydrogenase and heat-denatured glucose-6-phosphate dehydrogenase.

129 tion method was evaluated to visually detect denatured glutathione peptides, utilizing gold nanoparti

130 on protocol, nuclear magnetic resonance, and denaturing gradient gel electrophoresis were used in the

131 determined by next-generation sequencing or denaturing high-performance liquid chromatography in a s

132 as performed with polymerase chain reaction, denaturing high-performance liquid chromatography, and D

133 undiluted sera and SAB with greater than 30% denatured HLA (r = 0.40) and highest using diluted sera

134 using diluted sera and SAB with 30% or less denatured HLA (r = 0.86).

135 Antibody level, complement interference, and denatured HLA class I on SAB may all affect the clinical

136 Increasing levels of denatured HLA interfered with the detection of C1q bindi

137 single-antigen beads (SAB) is influenced by denatured HLA on SAB, antibody titre, and complement int

138 Conformational HLA and denatured HLA protein levels on SAB were determined usin

139 Denatured HLA was expressed as HC-10 binding to untreate

140 Despite this strong attraction, HNG does not denature IAPP.

141 exhibited significantly altered mobility in denatured immunoblots of CaValpha2delta1 G1060I and CaVa

142 -exclusion chromatography, and nondenaturing/denaturing immunoblotting techniques.

143 DNA duplexes are effectively denatured in alkaline solutions; whereas, the denatured

144 Membrane proteins denatured in SDS can also be refolded by addition of NIS

145 We find that DNA denatures in the majority of stretching simulations, sur

146 de; PLGA) microparticles (MPs) encapsulating denatured insulin (key self-antigen in type 1 diabetes;

147 The strong and usually denaturing interaction between anionic surfactants (AS)

148 Upon overexpression, SSA1 targets heat-denatured kinases toward degradation, which could enable

149 stem, HtpG enhanced native refolding of urea-denatured lactate dehydrogenase and heat-denatured gluco

150 ein complex GroEL) analyzed under native-MS, denaturing LC-MS, and positive and negative modes of ion

151 With denaturing LESA solvents, the highest mass protein detec

152 ted the ability of Hsp22 and Hsp70 to refold denatured luciferase in vitro.

153 Defects in the reactivation of heat-denatured luciferase were also evident.

154 Non-denaturing mass spectrometry revealed AmpR to be a homot

155 Using non-denaturing mass spectrometry, we show that McjD in compl

156 Glu-meso-DAP-d-Ala-d-Ala, as assessed by non-denaturing mass spectrometry.

157 centres on reversible folding of chemically denatured membrane proteins.

158 tile buffer components using an aqueous, non-denaturing mobile phase.

159 fied Mtb ClpB/DnaK system reactivated a heat-denatured model substrate, but the DnaJ HPD mutants inhi

160 ptides and mediates the reactivation of heat-denatured model substrates without the aid of the Hsp70

161 Mixing acid-denatured myoglobin with an aqueous ammonium acetate sol

162 ically, peptides and proteins that have been denatured or degraded can lead to adverse patient reacti

163 n turnover or during stress when kinases are denatured or improperly folded.

164 perform optimally on proteins analyzed under denatured or native-MS and solution conditions.

165 uded from the vicinity of a protein, whereas denaturing osmolytes such as urea lead to protein unfold

166 tides may alter other interactions of partly denatured p53 with receptors.

167 DL with CHO-SR-B1 cells were analyzed by non-denaturing PAGE, size-exclusion chromatography, and the

168 values to enable the generation of different denatured particles.

169 nsition from the native, folded state to the denatured, partly unfolded state was revealed with the a

170 Results showed that the addition of denatured pea protein to a wheat noodle matrix produced

171 Wheat noodles with added native or denatured pea-protein isolate were characterised for the

172 show excellent fragmentation efficiency for denatured peptides and proteins without the need to trap

173 nscription inhibitor and confirmed by freeze-denatured platelets being incapable of replicating the D

174 Denaturing polyacrylamide gel electrophoresis (SDS-PAGE)

175 ostructures using a combined approach of non-denaturing polyacrylamide gel electrophoresis, dynamic l

176 This effect was not impaired by a thermal denaturing process or blocked by the presence of anti-Fh

177 e of the double-stranded product followed by denaturing, produces a higher signal but the signal incr

178 small reduction of the fraction of the heat denatured protein aggregates.

179 e no correlation between the conformation of denatured protein and the release of individual peptides

180 imeric protein and the lower affinity of the denatured protein compared with type II collagen.

181 When a denatured protein immersed in electrolyte is driven thro

182 ted that the beta-Lactoglobulin was the most denatured protein in bovine whey powders regardless the

183 Content of acidic monosaccharides and denatured protein increased with increasing FG extractio

184 This denatured protein matrixes could be in part preventing s

185 be used to detect the primary structure of a denatured protein molecule.

186 oxoid formulation consistently outperforms a denatured protein preparation in all of the metrics stud

187 mbly reactions have involved the use of urea-denatured protein purified from inclusion bodies.

188 ained by subtracting spectra of unheated and denatured protein solutions at different temperature-tim

189 Comprehensive computer simulations of denatured protein translocation processes through the na

190 The acid denatured protein was digested with pepsin and analyzed

191 were bound covalently to the native and heat denatured protein.

192 nt intermolecular interactions to desolvate (denature) protein chains.

193 atrix with concentrated hydrochloric acid to denature proteins and allow the release of all arsenic s

194 Most AAA+ remodeling motors denature proteins by pulling on the peptide termini of f

195 compared under conditions typically used to denature proteins, i.e., thermal and chemical denaturati

196 s a quantitative analysis of FA's ability to denature proteins.

197 reference data set that contains soluble and denatured proteins (SDP48).

198 ion mobility mass spectrometry of native and denatured proteins and compared with those obtained by d

199 lex interactions between gelatinized starch, denatured proteins and lipids.

200 Denatured proteins are first focused and separated in a

201 predicted to remain above 3 kcal mol(-1) for denatured proteins as large as 900 residues.

202 making it possible to release and refold SDS-denatured proteins by adding sufficient amounts of NIS,

203 The thermally denatured proteins exposed different functional groups l

204 interaction and an increased contribution of denatured proteins in endolysosomal compartments, indica

205 1 and Sis1, and do not catalyze refolding of denatured proteins in vitro in cooperation with Ydj1 and

206 ment of this inherent dynamics in chemically denatured proteins is extremely challenging due to vario

207 experimental values RG and RE For chemically denatured proteins we obtain mutual consistency in our i

208 The denatured proteins were used as substrate for tryptic hy

209 It was hypothesised that denatured proteins with an unfolded structure, would hav

210 ults show that it was not possible to refold denatured proteins with concomitant ligand binding (neit

211 ce data suitable for comparison with data of denatured proteins, we repeated the assignment in 7 M ur

212 rils in microorganisms and animals, and many denatured proteins.

213 ts of FAIMS for the analysis of peptides and denatured proteins.

214 ilar behavior as synthetic polymers and urea-denatured proteins.

215 haperone critical for the folding of new and denatured proteins.

216 ions are suited for the detection of pGlu in denatured proteins.

217 Additionally, standard PAGE denatures proteins prior to analysis precluding determin

218 can prevent the irreversible aggregation of denaturing proteins.

219 eover, some of them can act on the capsid by denaturing proteins.

220 PEF treatment at 25 kV/cm at 50 degrees C denatures Pru p 3.

221 ctural and dynamics changes in SDS- and acid-denatured pSRII, thus highlighting differences in the un

222 The native and denatured RBP fractions were hydrolyzed with papain and

223 using both protein from inclusion bodies and denatured recombinant protein from E. coli membranes.

224 The reacted protein was then isolated, denatured, reduced, alkylated, and digested, and peptide

225 e conjugate are compared to those derived by denaturing reversed phase liquid chromatography using an

226 The fact that sonication-denatured rh-HGF had significantly lower uptake in U87MG

227 The results suggest that trypsin-hydrolyzed denatured rice bran albumin might be useful as a natural

228 lations demonstrate that the reversal in the denaturing role of pyridine is specific, and is related

229 Using non-denaturing separations and mass spectrometry identificat

230 enatured in alkaline solutions; whereas, the denatured single-stranded DNA strands readily reform dup

231 Furthermore, the measurements of thermally denatured single-stranded DNA were carried out and the v

232 high-throughput analytical platform based on denaturing size-exclusion ultraperformance liquid chroma

233 oup from myoglobin occurs as a result of the denaturing solution used.

234 espectively, when these ions are formed from denaturing solutions (water/methanol/acetic acid).

235 of these composite materials using chemical denaturing solutions.

236 heir branched-cyclic topoisomers prepared in denaturing solvent conditions with and without sulfolane

237 he native state is no longer stable, such as denaturing solvents and high protein concentrations wher

238 rong transmission voltages, by spraying from denaturing solvents, or trapping for extended periods of

239 salt-stimulated lipase (BSSL) and partially denaturing some of its proteins, potentially affecting i

240 involving the native state and unstructured denatured species.

241 ble surface area for unfolding, to probe the denatured state as a function of force, temperature, and

242 expose similar amounts of surface area, the denatured state at high temperature and low urea concent

243 eGFP-promoted entropic stabilization of the denatured state ensemble of EnHD.

244 the folding nucleus tend to interact in the denatured state in a transient manner and not simultaneo

245 Simulations over longer timescales, when the denatured state is less structured, do not show the anti

246 We thus find that the hot denatured state is more compact and richer in secondary

247 We find that the compaction of the denatured state is related to changing temperature as th

248 ee energy cost to form a trefoil knot in the denatured state of a polypeptide chain of 120 residues i

249 While it is widely appreciated that the denatured state of a protein is a heterogeneous conforma

250 However, whereas the compact denatured state of rhodanese is encapsulated efficiently

251 high denaturant indicates that any residual denatured state structure minimally affects the stabilit

252 old from either the knotted or the unknotted denatured state to characterize the free energy profile

253 with reference to the knotted and unknotted denatured state we find that knotting the polypeptide ch

254 richer in secondary structure than the cold denatured state, since water at lower temperatures can f

255 wn to be already lowly populated in the urea-denatured state.

256 nergy cost of forming a knot randomly in the denatured state.

257 ed (intermediate) --> irreversible unfolded (denatured) state, we study the thermal denaturation of h

258 ns (urea, force, and temperature) affect the denatured-state ensemble.

259 indicating that this part of the protein has denatured-state-level structure in the transition state.

260 Recently, the SDS-denatured states and the kinetics for reversible unfoldi

261 find that while the urea- and force-induced denatured states expose similar amounts of surface area,

262 Whereas in other proteins the cold-denatured states have been found to be slightly more com

263 mpts to refold DsbB and GlpG from chemically denatured states into lipid membranes resulted in extens

264 amics of different folding intermediates and denatured states is strongly connected to the exploratio

265 or to the first detailed characterisation of denatured states of a membrane protein by solution-state

266 this goal, we characterise the cold and hot denatured states of a protein by modelling NMR chemical

267 uctural differences between the hot and cold denatured states result in two alternative folding mecha

268 ll-length proteins from artificially induced denatured states that are far removed from the natural c

269 de series (AAXAA) as a model of unfolded and denatured states to examine the intrinsic propensities o

270 nd to be slightly more compact than the heat-denatured states, these two states occupy similar volume

271 ted sensitively and specifically without any denaturing step.

272 ives a qualitative picture of the collapsed, denatured structure.

273 e was detected in cataract lenses along with denatured structures.

274 release of components of the fuel (e.g., the denaturing substances) was also detected in the chamber

275 lters the conformational distribution of the denatured substrate protein rhodanese.

276 ATP or ADP and are therefore unable to fold denatured substrates such as alpha-lactalbumin, malate d

277 ulations for probing the ESI mechanism under denatured supercharging conditions.

278 ts importance to maintaining microbiomes and denaturing tannins.

279 cence shows that this domain undergoes a non-denaturing temperature-dependent transition with a high

280 n detergents such as octylglucoside are more denaturing than long chain detergents such as dodecylmal

281 mobilized such that specific receptor forces denature the duplex and thus expose a blocked primer.

282 ities to remove lignin catalytically without denaturing the carbohydrate moiety have enabled the conc

283 silica "cage", rendering them stable against denaturing thermal treatment and long-term ambient-tempe

284 embles of conformations corresponding to the denatured, transition, and native states in the folding

285 moted the oxidative folding of a reduced and denatured TSR in vitro.

286 Following each CAPTR event, denatured ubiquitin ions (13+ to 6+) yield products that

287 tions (CAPTR) to reduce the charge states of denatured ubiquitin ions in the gas phase, and ion mobil

288 nditions is comparable to that of the GdnHCl denatured unfolded state.

289 Glutathione was denatured using a 70 degrees C water bath to create an a

290 ing the oxygenated form of rHb Kirklareli to denature very rapidly under physiological conditions.

291 Following stringent denaturing washes, the resulting covalently linked prote

292 No separate aggregation of kappa-casein/denatured whey protein complexes or kappa-casein deplete

293 n and the concentrations of kappa-casein and denatured whey protein in the serum, and a reduction in

294 on were characterised and loaded into a heat-denatured whey protein isolate (WPI) solution which was

295 347nm were fabricated and added into a heat-denatured whey protein solution.

296 f total whey protein, partial association of denatured whey protein with the casein micelle, an incre

297 ation to pH 5.4, the serum phase caseins and denatured whey proteins partially reassociated with the

298 At pH below 5.4 the caseins and denatured whey proteins rapidly aggregated.

299 s resulted in different levels of casein and denatured whey proteins to be distributed between the co

300 sical and probiotic yogurt the totals of non-denatured whey proteins were 92.31 and 91.03%.