ライフサイエンスコーパス: denaturing

1 tic pressure inside the NMR sample cell from denaturing (2.5 kbar) to native (1 bar) conditions.

2 ure proteins, the molecular mechanism of its denaturing ability is still a subject of considerable de

3 e adapted and used a highly stringent Tandem Denaturing Affinity Purification (TDAP) method, coupled

4 Apple PPO was thermolabile, denaturing after 10min at 70 degrees C.

5 NA-capsids are based on two-dimensional, non-denaturing agarose gel electrophoresis, followed by stru

6 3 activity on full-length VWF arises without denaturing agent as evidenced by FCS and gel-based multi

7 Exposing the intracellular domain to a denaturing agent increases the energy required to open t

8 We show that denaturing agents can alter digestion by reducing protea

9 Use of alkylureas as denaturing agents in capillary electrophoresis and cIEF

10 proaches studying the effect of proteases or denaturing agents on the physicochemical properties of P

11 ium, Tris), magnesium ions and commonly used denaturing agents such as formamide and DMSO.

12 ng amyloid fibrils formed in the presence of denaturing agents that significantly alter the folding s

13 l denaturation, complex coupling between two denaturing agents, and differential scanning calorimetry

14 les to elevated temperature, proteolytic and denaturing agents, and extend the operable pH range for

15 ilibrium unfolding, complex coupling between denaturing agents, characteristic DSC thermogram, gradua

16 high inherent susceptibility to reducing and denaturing agents, it is very stable upon immobilization

17 t during demineralization or with the use of denaturing agents.

18 3 microL) with 1.5 microg of trypsin without denaturing agents.

19 from its structured state in the absence of denaturing agents.

20 nto peptides by processes that use salts for denaturing and buffering purposes.

21 les cause structural changes including local denaturing and compaction to DNA under mild conditions.

22 distributions of globular proteins under non-denaturing and denaturing conditions.

23 acid and aspartic acid over time under both denaturing and native conditions at pH 8.2, we found tha

24 Using both denaturing and native gel analysis, we have identified n

25 Immunoprecipitation, cross-linking, and denaturing and native PAGE analyses of Gal80 in vitro an

26 eriments on ubiquitin ions ionized from both denaturing and native solution conditions suggest that r

27 the ability to run the samples in different denaturing and nondenaturing buffer allows defining true

28 nstrated across pH 3.8-7.0 muIPGs using both denaturing and nondenaturing conditions, without the add

29 In silico analyses, denaturing and nondenaturing gel Western blot, and immun

30 ent PRDC dimers are highly stable under both denaturing and reducing conditions.

31 ing and dissociation under native, partially denaturing, and fully unfolded conditions indicate that

32 peanut, and pistachio flours using three non-denaturing aqueous (phosphate, borate, and carbonate) bu

33 single and interactive effects of three non-denaturing aqueous (phosphate, borate, and carbonate) bu

34 nstrate the relative stability of RNA in the denaturing/basic IEX mobile phase, lay out a protocol to

35 Non-denaturing bisulfite treatment modifies the cytosines on

36 A two-dimension matrix of pH and denaturing buffer conditions was screened to optimize na

37 ble immobilization (SPRI) beads with protein-denaturing buffers, and then identified by proteomics.

38 The extent of protein CSD shifting/denaturing can be tailored by acid identity and concentr

39 We find that MoS2 exhibits robust denaturing capability to HP35, with its secondary struct

40 or large populations may be discovered using denaturing capillary electrophoresis (DCE).

41 dinarily would decompose as a consequence of denaturing chromatographic conditions typically used for

42 ve, ATP-independent chaperones that can bind denaturing client proteins to prevent their irreversible

43 he polymer properties of Gln repeats even in denaturing concentrations of GdnHCl.

44 t from studying enzyme conformations under a denaturing condition, it is highly informative to manipu

45 sed conformational space sampling under urea-denaturing condition.

46 ta2m (hbeta2m) forms amyloid-like fibrils in denaturing conditions (e.g. pH2.5) in the absence of NaC

47 trast, globular proteins unfolded under less denaturing conditions (via mutations, or transiently unf

48 over, the acquisition of full MS scans under denaturing conditions allowed profiling of all abundant

49 ion auxiliaries that are removed under harsh denaturing conditions and have limited applicability.

50 We find that even under strongly denaturing conditions and in the absence of residual sec

51 and purified a recombinant form of p33 under denaturing conditions and optimized conditions for the r

52 ic activity over the same RNAs, purified via denaturing conditions and refolded.

53 mentary procedures, which use strong protein denaturing conditions and yield highly purified core and

54 study suggests that protein losses under non-denaturing conditions are more sensitive to protein stru

55 In addition, under non-denaturing conditions AV-1980R/A sera preferentially rec

56 ization method was developed using native or denaturing conditions by direct infusion into an Exactiv

57 We find that mild denaturing conditions can affect the sensitive balance b

58 g those obtained under traditional native or denaturing conditions can be reversibly obtained simply

59 ha-synuclein isolated and analysed under non-denaturing conditions from neuronal and non-neuronal cel

60 Deriving equations under denaturing conditions has several pitfalls, and here, we

61 r contacts has been studied under a range of denaturing conditions in single domains of the immunoglo

62 re the behavior of proteins under native and denaturing conditions in the presence of NaCl, CsCl, and

63 tional method to an IgG1 antibody under mild denaturing conditions indicates that the unfolding of ea

64 This suggests that denaturing conditions mimic good solvents, whereby the p

65 Thus, 30% SDS and 3% SDS + 8 M urea are the denaturing conditions of choice to study maximally unfol

66 ORF2 purified from bacteria under denaturing conditions preferentially interacted with dou

67 The sizes of unfolded proteins under highly denaturing conditions scale as N(0.59) with chain length

68 By purifying under denaturing conditions this method not only reduces the r

69 e of protein ESI mass spectrometry under non-denaturing conditions to analyze a dynamic combinatorial

70 quently, affinity purification is used under denaturing conditions to capture proteins conjugated wit

71 Comparing non-denaturing conditions to FDF-PAGE uncovered extensive se

72 Because of the denaturing conditions used, this method ultimately leads

73 ectric focusing (cIEF) is normally run under denaturing conditions using urea to expose any buried pr

74 or which the SETA reactivity increased under denaturing conditions were found to engage in tertiary p

75 ints imposed by the gel pores such that more denaturing conditions were required within the gel than

76 noncyclic SST possess greater resistance to denaturing conditions with decreased monomer releasing p

77 d when the samples were loaded in moderately denaturing conditions with multiple loading-washing cycl

78 However, under mildly denaturing conditions, a ligand concentration-dependent

79 cally disordered proteins, or proteins under denaturing conditions, and studies of amyloid aggregate

80 common instrumentation, is carried out under denaturing conditions, and takes less than 30min.

81 itor oxidation in full-length proteins under denaturing conditions, as demonstrated on two biotherape

82 olded states can be populated under strongly denaturing conditions, but the ensemble which is relevan

83 Under mildly denaturing conditions, CysC forms inactive domain-swappe

84 ssessed by immunoblotting under reducing and denaturing conditions, deconjugation-resistant LC3B accu

85 Under strongly denaturing conditions, folding was found to follow a con

86 onjugates by purification of 6His-SUMO under denaturing conditions, followed by western blotting for

87 These techniques, generally run under denaturing conditions, have proven to be only marginally

88 Under denaturing conditions, limited membrane digestion of bov

89 Since solid-phase purification under protein-denaturing conditions, rather than sequence-specific nuc

90 tive conditions at pH 8.2, we found that, at denaturing conditions, succinimide at light chain residu

91 e more stable than the native ensemble under denaturing conditions, such as high temperature.

92 microtubules by paclitaxel, laulimalide, or denaturing conditions, suggesting irreversible binding o

93 Following purification under denaturing conditions, SUMOylated proteins were identifi

94 After exposure to denaturing conditions, the C-terminal fragment of LPL re

95 Surprisingly, even under denaturing conditions, the energy landscape remains high

96 Using denaturing conditions, the hemoglobin (Hb) tetramer diss

97 inguishable from that of amyloid grown under denaturing conditions, where the C-terminal alpha-helica

98 e RNA interactions, and it is purified under denaturing conditions.

99 yaA has usually been extracted and stored in denaturing conditions.

100 proteins is isolated by centrifugation under denaturing conditions.

101 ntermediate states under a variety of mildly denaturing conditions.

102 proteins from E. coli cell lysates under non-denaturing conditions.

103 conditions and aggregation experiments under denaturing conditions.

104 rmation by MR in aqueous solutions under non-denaturing conditions.

105 yet durably retain their targets even under denaturing conditions.

106 aled a single conformational ensemble at all denaturing conditions.

107 dopt a completely disordered structure under denaturing conditions.

108 no acids to promote residual structure under denaturing conditions.

109 +) were restored to inactive PS under mildly denaturing conditions.

110 in the fully folded state except under harsh denaturing conditions.

111 f globular proteins under non-denaturing and denaturing conditions.

112 enhances the stability of that enzyme under denaturing conditions.

113 urant concentrations and deviated under less denaturing conditions.

114 ted by monitoring the change in A(230) under denaturing conditions.

115 criteria, although they were purified under denaturing conditions.

116 iols, amines, and acids in high purity under denaturing conditions.

117 ility to modulate the structure of TTR under denaturing conditions.

118 sulfhydryl (-SH) content on applying mildly denaturing conditions.

119 essential to retain triplex structure under denaturing conditions.

120 y stable at high temperatures and even under denaturing conditions.

121 e precipitation of the modified strand under denaturing conditions.

122 e unfolding pathways under the two different denaturing conditions.

123 nd was recovered only after extraction under denaturing conditions.

124 microscopy and biochemical assays under non-denaturing conditions.

125 as required cofactors and/or unphysiological denaturing conditions.

126 tion), which are not detected under standard denaturing conditions.

127 pe VWF in the absence of shear but partially denaturing conditions.

128 their activities under thermal and chemical denaturing conditions.

129 aggregates they formed, as revealed by semi-denaturing detergent agarose gel electrophoresis.

130 tissues, even without a target; therefore, a denaturing detergent buffer is necessary for exhaustive

131 between KS and a protein's resistance to the denaturing detergent SDS, we show here the application o

132 e to disruption of protein immobilization by denaturing detergents (SDS) and incubation at elevated t

133 n detergents such as heptylthioglucoside and denaturing detergents such as SDS.

134 After extraction with non-denaturing detergents, we affinity-purified 785 endogeno

135 ators across various bottom-up assays (i.e., denaturing/digestion conditions).

136 to online mass spectrometry or result in the denaturing dissociation of conjugated HC and LC during c

137 site induces directed loop migration toward denaturing domains, a phenomenon that merges destabilizi

138 melting point depressants capable of rapidly denaturing double stranded DNA (dsDNA) linkers, and 8 co

139 developed in the past decades, all involving denaturing dsDNA and hybridizing fluorescent nucleic aci

140 n binding on the channel surface and protein denaturing during device assembly.

141 mbrane, provides a rationale for the general denaturing effect of the CL surface and the large-scale

142 nd trimethylamine N-oxide can counteract the denaturing effect of urea.

143 ears sufficient to explain the thermodynamic denaturing effect of urea.

144 in marine organisms against the detrimental denaturing effects of another naturally occurring osmoly

145 he Tau protein, that TMAO can counteract the denaturing effects of urea by inhibiting protein-urea pr

146 wed by separating the cleavage products on a denaturing electrophoresis gel to identify the protein-b

147 platform is compatible with both native and denaturing electrospray, operates with a throughput of u

148 de the suitable electrostatic charge and non-denaturing environment for enzyme immobilization, but it

149 ion under excursions in temperature, pH, and denaturing environment.

150 These denaturing environments are thought to be good solvents

151 Therefore, typical denaturing environments cannot be classified as good sol

152 effects lead to conformational statistics in denaturing environments that are congruent with those of

153 then do generic protein sequences expand in denaturing environments?

154 extent that it accumulates even under mildly denaturing equilibrium conditions.

155 simulation in water, we found six transient denaturing events near the melting temperature (323 and

156 The native and denaturing extraction protocols have captured 1109 orang

157 cAMP binding stabilizes the CBD-A against a denaturing force, and increases the folded state lifetim

158 this problem, we established FDF-PAGE (fully-denaturing formaldehyde polyacrylamide gel electrophores

159 on of the labeled small RNAs is performed by denaturing gel electrophoresis and autoradiography or ph

160 ad of oxidative DNA cleavage is confirmed by denaturing gel electrophoresis and surface group pKa mea

161 ulation of RNA molecules is separated by non-denaturing gel electrophoresis and the conformers are in

162 xes with linear RNA targets were detected by denaturing gel electrophoresis and were found to be more

163 involves the separation of RNA molecules by denaturing gel electrophoresis followed by transfer and

164 Following EMSA, denaturing gel electrophoresis is implemented to provide

165 variants within the gene were determined by denaturing gel electrophoresis or automated capillary-ba

166 lpha-syn to these standards using native and denaturing gel electrophoresis techniques, size-exclusio

167 ll lysates and intact cells and by using non-denaturing gel electrophoresis without cross-linking, th

168 Here, we present non-denaturing gel electrophoresis, UV melting, and circular

169 l proteins were extracted and resolved using denaturing gel electrophoresis.

170 f the synapse of two site Is observed by non-denaturing gel electrophoresis.

171 spectrometry at approximately 50 kDa and by denaturing gel filtration at approximately 50-61 kDa, wh

172 CSalpha to retain its 32P throughout the SDS denaturing gel process was unique over the entire mitoch

173 fy the functional form of TBCD and using non-denaturing gels and immunoblotting, we analyzed lysates

174 on immunoblots of blue-native gels, whereas denaturing gels and immunocytochemistry showed reduced c

175 Filtration assays and non-denaturing gels demonstrated that Abeta oligomers cross-

176 and of overexpression, sample heating and/or denaturing gels for cell culture and tissue studies.

177 sured for individual tRNA species using acid denaturing gels, or comparatively at the genome level us

178 resulting in a significant mobility shift on denaturing gels.

179 ow charging levels were validated using acid denaturing gels.

180 ring transition-metal ions, inducing protein denaturing, generating air bubbles or making pores in me

181 Denaturing Gradient Gel Electrophoresis (DGGE) analysis

182 munities and T. versicolor was studied using denaturing gradient gel electrophoresis (DGGE) and the c

183 ntial isolation and eubacterium-specific PCR-denaturing gradient gel electrophoresis (DGGE) in conjun

184 Denaturing gradient gel electrophoresis (DGGE) of the cl

185 t bacterial community structure derived from denaturing gradient gel electrophoresis (DGGE) shifted m

186 Plate counts, Denaturing Gradient Gel Electrophoresis (DGGE), short-ch

187 Denaturing gradient gel electrophoresis (DGGE), which co

188 phyton associated microbial consortium using denaturing gradient gel electrophoresis (DGGE).

189 nd sequencing or community fingerprinting by denaturing gradient gel electrophoresis (DGGE).

190 Gut microbiota profiling by denaturing gradient gel electrophoresis and 454/FLX-base

191 submitted to polymerase chain reaction(PCR)-denaturing gradient gel electrophoresis and real-time qu

192 etermined by principal component analysis of denaturing gradient gel electrophoresis profiles was evi

193 dant with ITS lineage membership (defined by denaturing gradient gel electrophoresis screening), yet

194 Denaturing gradient gel electrophoresis similarly reveal

195 on protocol, nuclear magnetic resonance, and denaturing gradient gel electrophoresis were used in the

196 s of the biocides applied, by using cloning, denaturing gradient gel electrophoresis, and culture-dep

197 traviolet hypochromicity methods, as well as denaturing gradient gel electrophoretic methods.

198 mutations were detected by Sanger sequencing/denaturing high performance liquid chromatography (Ss/DH

199 Using denaturing high performance liquid chromatography, massi

200 Denaturing high-performance liquid chromatography (DHPLC

201 Using denaturing high-performance liquid chromatography (DHPLC

202 PCR-amplified samples were pre-screened by denaturing high-performance liquid chromatography and co

203 A (NaV1.5, LQTS type 3) were performed using denaturing high-performance liquid chromatography and di

204 xcluding acute promyelocytic leukemia, using denaturing high-performance liquid chromatography and di

205 riations in the ATM gene by a combination of denaturing high-performance liquid chromatography and di

206 xon, followed by heteroduplex analysis using denaturing high-performance liquid chromatography and di

207 obtained and screened for SCN5A mutations by denaturing high-performance liquid chromatography and se

208 samples from 470 cases of isolated CHD using denaturing high-performance liquid chromatography and se

209 and FBXW7 were screened by a combination of denaturing high-performance liquid chromatography and se

210 ATM status was analyzed by denaturing high-performance liquid chromatography and wa

211 determined by next-generation sequencing or denaturing high-performance liquid chromatography in a s

212 Denaturing high-performance liquid chromatography was us

213 Denaturing high-performance liquid chromatography was us

214 as performed with polymerase chain reaction, denaturing high-performance liquid chromatography, and d

215 Using polymerase chain reaction, denaturing high-performance liquid chromatography, and D

216 n of ANKRD1 using polymerase chain reaction, denaturing high-performance liquid chromatography, and d

217 as performed with polymerase chain reaction, denaturing high-performance liquid chromatography, and D

218 ombination of ABCR400 genotyping microarray, denaturing high-performance liquid chromatography, and h

219 sets of mutation accumulation lines by using denaturing high-performance liquid chromatography.

220 -exclusion chromatography, and nondenaturing/denaturing immunoblotting techniques.

221 d by cutting the protease site with trypsin, denaturing in guanidine hydrochloride to disrupt the com

222 ogenesis may occur directly from assembly of denaturing insulin monomers rather than by successive tr

223 The strong and usually denaturing interaction between anionic surfactants (AS)

224 ein complex GroEL) analyzed under native-MS, denaturing LC-MS, and positive and negative modes of ion

225 With denaturing LESA solvents, the highest mass protein detec

226 This is achieved by introducing a denaturing lysis step (in the presence of sodium dodecyl

227 Non-denaturing mass spectrometry analysis and analytical ult

228 Non-denaturing mass spectrometry revealed AmpR to be a homot

229 a prerequisite before their analysis by the denaturing mass spectrometry technique.

230 Using non-denaturing mass spectrometry, we show that McjD in compl

231 Glu-meso-DAP-d-Ala-d-Ala, as assessed by non-denaturing mass spectrometry.

232 lude that large complex RNAs prepared by non-denaturing means form a homogeneous population and are c

233 lytically more active than those prepared by denaturing methods and subsequent refolding; this facile

234 tile buffer components using an aqueous, non-denaturing mobile phase.

235 the peptides, a phenomenon akin to the cold denaturing of some proteins.

236 -syn expressed in E. coli and purified under denaturing or nondenaturing conditions, whether as a fre

237 Urea is known as a common denaturing osmolyte that affects protein function by des

238 g osmolyte trimethylamine-N-oxide (TMAO) and denaturing osmolyte urea for the case of alpha-synuclein

239 Urea, a denaturing osmolyte, shifts the equilibrium toward U; tr

240 uded from the vicinity of a protein, whereas denaturing osmolytes such as urea lead to protein unfold

241 o1, we performed chemical cross-linking, non-denaturing PAGE, and electromobility shift assays, which

242 DL with CHO-SR-B1 cells were analyzed by non-denaturing PAGE, size-exclusion chromatography, and the

243 We generated various hybrids by denaturing pairs of enzymes in 1 M guanidinium chloride

244 Denaturing polyacrylamide gel electrophoresis (SDS-PAGE)

245 Co-affinity purification, non-denaturing polyacrylamide gel electrophoresis and bis(ma

246 uniquely 3'-radiolabeled DNA substrates and denaturing polyacrylamide gel electrophoresis to provide

247 circular dichroism spectroscopy, native and denaturing polyacrylamide gel electrophoresis, and UV-vi

248 ostructures using a combined approach of non-denaturing polyacrylamide gel electrophoresis, dynamic l

249 inked duplexes that were readily detected on denaturing polyacrylamide gels.

250 However, the mechanism behind its denaturing power is still not well understood.

251 This effect was not impaired by a thermal denaturing process or blocked by the presence of anti-Fh

252 e of the double-stranded product followed by denaturing, produces a higher signal but the signal incr

253 micelles are the most common choice, but the denaturing properties make them unsatisfactory for studi

254 duced cell damage by binding and maintaining denaturing proteins in a folding-competent state.

255 independent chaperones to bind and stabilize denaturing proteins that can be later reactivated by ATP

256 bility to behave as an unfoldase, repeatedly denaturing proteins upon binding, which in turn can free

257 These findings suggest that, in denaturing proteins, urea (and perhaps other denaturants

258 can prevent the irreversible aggregation of denaturing proteins.

259 urea has been commonly used as an agent for denaturing proteins.

260 eover, some of them can act on the capsid by denaturing proteins.

261 n the pH and temperature and the presence of denaturing reagents.

262 -micellar and sub-denaturing to micellar and denaturing, revealed multiple complexes of UBI and SDS o

263 e conjugate are compared to those derived by denaturing reversed phase liquid chromatography using an

264 lations demonstrate that the reversal in the denaturing role of pyridine is specific, and is related

265 formed complexes as detected by reducing and denaturing SDS-PAGE.

266 Upon partial denaturing, seeds consisting of 3-4 monomers quickly app

267 Using non-denaturing separations and mass spectrometry identificat

268 fied transition states from high-temperature denaturing simulations.

269 high-throughput analytical platform based on denaturing size-exclusion ultraperformance liquid chroma

270 oup from myoglobin occurs as a result of the denaturing solution used.

271 espectively, when these ions are formed from denaturing solutions (water/methanol/acetic acid).

272 reater than those obtained from conventional denaturing solutions consisting of water/methanol/acid,

273 ces are less sensitive to solvent quality in denaturing solutions of GdnHCl than Gly-rich sequences.

274 arging appear similar to those obtained from denaturing solutions where charging beyond the total num

275 supercharging from native solutions and from denaturing solutions.

276 of these composite materials using chemical denaturing solutions.

277 heir branched-cyclic topoisomers prepared in denaturing solvent conditions with and without sulfolane

278 when electrospraying unfolded proteins in a denaturing solvent.

279 he native state is no longer stable, such as denaturing solvents and high protein concentrations wher

280 rong transmission voltages, by spraying from denaturing solvents, or trapping for extended periods of

281 salt-stimulated lipase (BSSL) and partially denaturing some of its proteins, potentially affecting i

282 At the initial heat-denaturing step and during the PCR sequence, the 3'-prot

283 ted sensitively and specifically without any denaturing step.

284 in synthesis or during cellular responses to denaturing stress.

285 rganic ions and urea, it imposes osmotic and denaturing stresses on bacterial cells.

286 release of components of the fuel (e.g., the denaturing substances) was also detected in the chamber

287 ts importance to maintaining microbiomes and denaturing tannins.

288 cence shows that this domain undergoes a non-denaturing temperature-dependent transition with a high

289 Here, we show that the denaturing temperatures of both hen egg white lysozyme a

290 template amyloid growth at physiological vs denaturing temperatures.

291 n detergents such as octylglucoside are more denaturing than long chain detergents such as dodecylmal

292 ities to remove lignin catalytically without denaturing the carbohydrate moiety have enabled the conc

293 eavy chain on an antibody were eliminated by denaturing the protein, revealing that structural elemen

294 free energy of binding by about 30% without denaturing the protein, suggesting that hydrophobic forc

295 silica "cage", rendering them stable against denaturing thermal treatment and long-term ambient-tempe

296 ons of SDS ranging from sub-micellar and sub-denaturing to micellar and denaturing, revealed multiple

297 emains unclear whether in the absence of any denaturing treatment, PrP-lipid interaction is sufficien

298 ational epitopes remain functional following denaturing treatments and indicate that cellular PrP and

299 Following stringent denaturing washes, the resulting covalently linked prote

300 chloride IL was discovered to be capable of denaturing wool and was determined to be the most effect