ライフサイエンスコーパス: desalt

1 the membrane to small ions allows efficient desalting.

2 MS analysis of the digestion mixture without desalting.

3 er demanding applications without additional desalting.

4 iring toxic demulsifiers and water-intensive desalting.

5 thin materials for molecular separations or desalting.

6 o those from conventional guanidination with desalting.

7 asured directly from reaction mixtures after desalting.

8 The mass of the desalted ADC is subsequently determined using standard d

9 d an online cIEF-ESI-MS interface to reduce (desalt) amino acid ampholytes in-line after cIEF and pri

10 utomated direct sequencing and genotyping of desalted amplification products.

11 With our experimental CDI cell, we desalted an electrolyte consisting of equimolar potassiu

12 bsystems to multiplex sample loading, online desalting, analysis, and column regeneration.

13 ercial guard column in an off-chip format to desalt and concentrate the products of the enzymatic rea

14 cation exchange filtration were utilized to desalt and preconcentrate seawater DOM prior to nonspeci

15 hase extraction (SPE) tips have been used to desalt and purify proteins and peptides from mixtures of

16 ness, with fwhm as small as 3.7% achieved in desalted and acidified solutions.

17 philic peptides at the picomole level can be desalted and characterized by matrix-assisted laser deso

18 ntinuous stream of sea water is divided into desalted and concentrated streams by ion concentration p

19 Glycopeptides were desalted and concentrated using solid-phase extraction a

20 n the ED unit, the diluted draw solution was desalted and high-quality water was produced; the concen

21 The mass spectra of peptides desalted and washed on uncoated stainless steel MALDI pl

22 conventional approach, samples are typically desalted and/or concentrated with various techniques and

23 Rapid desalting and a low volume connection to an electrospray

24 ed using the described protein precipitation/desalting and cation-exchange solid-phase extraction iso

25 ents: (1) isolation by protein precipitation/desalting and cation-exchange solid-phase extraction, (2

26 s were identical regardless of the method of desalting and concentrating of protein samples, samples

27 e for in-well trypsin digestion, followed by desalting and concentrating the digestion products in th

28 ries of sample handling processes, including desalting and concentrating, to be performed directly on

29 ause of the post-electrophoresis extraction, desalting and concentration steps.

30 Desalting and concentration takes approximately 5 min wh

31 w effectively eliminated the need for sample desalting and enabled compatible sample processing for m

32 r most molecular chemical techniques, sample desalting and enrichment is a prerequisite.

33 onable analytical procedures with respect to desalting and enrichment were established.

34 To ensure an efficient electrospray process, desalting and exchanging the biomolecular solutions into

35 usion chromatography (muSEC) module for fast desalting and its integration into microfluidics, along

36 All digestates were then combined for desalting and MALDI-TOF/TOF mass spectrometric analysis.

37 Development of methods for desalting and MS/MS fragmentation allows rapid acquisiti

38 l processing steps on a small scale, such as desalting and protein digestion, could be developed and

39 ked with reversed-phase material for on-chip desalting and reversed-phase chromatography.

40 easurements reveal characteristic regimes in desalting and salting modes and demonstrate that the rat

41 Desalting and sample concentration follows each fraction

42 (nRapidFire-MS) capable of performing online desalting and sample introduction in as little as 10 s p

43 ammalian cells, surface adhesion, along with desalting and speed-vacuum drying steps, all contribute

44 applied to tryptic peptide mixtures prior to desalting and spotting onto MALDI-TOF plates.

45 eps involved in a sample preparation such as desalting and various chromatographic purification schem

46 otides at the most basic purification level (desalted) and without the need for costly and time-consu

47 column where it is concentrated and rapidly desalted, and (vi) peptides eluted from the dilute captu

48 Peak fractions were collected, desalted, and analyzed by high-resolution MS using a top

49 r redox co-reactants, reaction mixtures were desalted, and MALDI-TOF MS was used to monitor both time

50 hophores and chlorophytes, was concentrated, desalted, and nebulized to form aerosol particles compos

51 g specific protein fractions to be selected, desalted, and subsequently spray-dried onto the QCM for

52 ctions is the time-consuming chromatography, desalting, and concentration steps required to prepare a

53 n steps, including depletion, fractionation, desalting, and concentration.

54 e concentration, buffer exchange, digestion, desalting, and matrix/analyte cocrystallization for MALD

55 ed 16-plex TMT, including labeling reaction, desalting, and MS conditions, and then directly compared

56 buffer exchange, protease digestion, peptide desalting, and, in the case of MALDI-MS, matrix and anal

57 general, and highly efficient, rapid in-line desalting approach using a small gel cartridge to assist

58 For these reasons, the sample is generally desalted before mass analysis.

59 Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and sto

60 The GAGs were desalted by membrane filtration and analyzed in dilute f

61 Simulated biological samples, desalted by ultrafiltration in the presence gamma-CD, we

62 lar masses were apparently lost from samples desalted by ZipTip(C18) microcolumns, thus diminishing t

63 We compared trysin-digested protein samples desalted by ZipTip(C18) reverse-phase microcolumns with

64 rotein A affinity chromatography followed by desalting by gel filtration and was characterized by ami

65 Following purification and desalting by reversed-phase HPLC, buffer exchange with M

66 o collect CIU fingerprints in 30 s following desalting by using size exclusion chromatography cartrid

67 yzed carbohydrates by replacing ion-exchange desalting cartridges with evaporative removal of HCl und

68 ddition, it provides an effective method for desalting cellular RNA samples having complex matrixes,

69 proteomic analysis through automated sample desalting/cleanup and enrichment of target mass ranges (

70 elds, and product purification using a PD-10 desalting column.

71 I-CIU, among which are (i) improved and fast desalting compared to manual buffer exchange used for cl

72 ive crabs, and the resulting dialysates were desalted, concentrated, and analyzed by LC-ESI-QTOF and

73 actions from a trypsinized yeast lysate were desalted, concentrated, and analyzed in a completely aut

74 The extension products are desalted, concentrated, and subjected to delayed-extract

75 e antibody-drug conjugate (ADC) using native desalting conditions, we maintain the intact bivalent st

76 l volume 2.5 muL) described here effectively desalted continuous flows of NaCl solutions (200 mequiv/

77 isobaric tag labeled glycopeptides after C18 desalting could be readily enriched by SAX and RAX cartr

78 tform for high-throughput and low sample-use desalting coupled with collision-induced dissociation (C

79 ases, we have evaluated a model system where desalted CS is combined with isolated human granzyme.

80 ium dodecyl sulfate (SDS) and following with desalting/delipidation of the sample by chloroform/metha

81 We have developed a microfabricated desalting device that meets both requirements, thus prov

82 the digestion products and then assessed the desalted digests by ESI-MS.

83 ts the analyte to be easily concentrated and desalted directly on the probe tip.

84 as been widely applied for concentrating and desalting DOM.

85 e traditional solvent-based method using the desalted "dry" MALDI preparation procedure.

86 atment, commercial LoBind tubes, and in-line desalting during sample processing.

87 Two-stage desalting (during solid phase extraction and on the anal

88 Desalting efficiency and analyte loss was evaluated with

89 Importantly, up to 98% desalting efficiency is achieved.

90 roximately 80% and demonstrate the exquisite desalting efficiency with high-performance electrospray

91 This approach allows effective sample desalting, enrichment, sequential elution, and MS detect

92 ving as proof-of-concept, demonstrate alphaS desalting, exchange buffer efficiency, and reduced solve

93 nein and ODA10p rebound to the axonemes when desalted extracts are mixed with oda10-mutant axonemes.

94 odes sandwiching a dielectric separator, and desalt feedwater flowing through the cell by storing ion

95 ne protein targets, we show that appropriate desalting followed by different folding strategies enabl

96 Samples in solution do not need to be desalted following reduction and alkylation, with excess

97 red to remove approximately 18% of the ions (desalt) from of the feed solution was observed to be sig

98 he procedure does not require alkylation and desalting, greatly shortening the sample preparation tim

99 into the complex protein matrix provided by desalted human urine ( approximately 1.5 muM total prote

100 The protein is first desalted into a suitable buffer at pH 8-9 and a molar ex

101 ator is removed and the protein conjugate is desalted into an acidic buffer.

102 can shift current utilization (ratio between desalted ions and ions conducted through electrodes) and

103 Desalting is not needed prior to MALDI-TOF MS.

104 edures are detrimental to MALDI-MS, and thus desalting is required before the digestion products can

105 strate ADP-dependent PPDK phosphorylation in desalted leaf extracts of the C(3) plants Vicia faba and

106 nd forty-five 7-ml fractions were collected, desalted, lyophilized, and resuspended.

107 This desalting method directly separates highly polar, ionic

108 This method provides a simple, versatile desalting method for measuring exometabolites of environ

109 MALDI-TOF, applying our successive on-plate desalting method that eliminates the insensitivity of th

110 uid chromatography-mass spectrometry (LC-MS) desalting method that mitigates these shortcomings.

111 sample preparation is improved using a novel desalting method that utilizes the hydrophobic surface o

112 ca columns; however, these entail subsequent desalting methodologies and consequent sample losses and

113 The most common desalting methods are faced with major limitations, incl

114 versed-phase liquid chromatography (IP-RPLC) desalting methods we previously employed do not effectiv

115 Its removal by various solid-phase desalting methods, catalase treatment, or freeze drying

116 We evaluated a range of desalting methods, including spin columns, dialysis memb

117 IMAC procedure was significantly improved by desalting methylated peptides, followed by gradient elut

118 Specifically for desalting NaCl, this enhancement of unipolar cation cond

119 In this study, we present a novel method for desalting of ADCs and mAbs building on the SP3 approach

120 Overall, the (2)D HILIC allowed online desalting of AEX and IPRP modes and further separation o

121 assess the feasibility of this approach, the desalting of alpha-synuclein (alphaS) was investigated u

122 octadecyl amine (ODA) to be employed in the desalting of complex mixtures and the results are compar

123 This protocol involves the desalting of nucleic acids using ammonium acetate precip

124 Desalting of protein solutions is demonstrated for ESI-M

125 Elimination of the need for desalting of samples after reaction raised the possibili

126 The complete desalting of samples, which is necessary for IEF, tends

127 Size-selective separation and desalting of small model molecules ( approximately 200-1

128 graphic media for the microconcentration and desalting of SUPREX samples.

129 phase purification approach also facilitates desalting of the captured oligonucleotides, which is ess

130 of salt adducts without the need for offline desalting or buffer exchange, and each multiplexed measu

131 spin-type kits are extensively used for the desalting or buffer-exchange of mAbs and ADCs samples.

132 gonal separation methods that do not require desalting or extensive sample handling.

133 ut the need for intermediate chromatography, desalting, or concentration steps.

134 e presence of excess MnCl2 followed by rapid desalting over a gel filtration column resulted in the r

135 n nonlinear increases in energy required for desalting over standard IEM devices due to electroosmoti

136 pid and fully automated method to purify and desalt PCR products prior to analysis by electrospray io

137 peptides in a sample that has nominally been desalted; peptides arising from nontryptic cleavage in a

138 nucleic acids, we have developed a nanoflow desalting platform for high-throughput and low sample-us

139 Online desalting platforms have enabled facile sample cleanup a

140 d, the samples can be evaporated rather than desalted, preventing substantial sample loss and allowin

141 The fractions from the ERLIC column were desalted prior to C-18-reversed phase liquid chromatogra

142 the molecular ion region of the analytes, a desalting procedure of the MBM sample directly on the MA

143 reby eliminating conventional time-consuming desalting procedures required for downstream analysis of

144 to be applied in field studies, eliminating desalting procedures, which can be time-consuming and no

145 electrospray emitters with submicron tips to desalt protein ions.

146 anes; the first one with MWCO below 5700 for desalting protein samples, and the second one with a hig

147 attractive alternative to other methods for desalting proteins prior to mass spectrometry analysis.

148 This tip-based purification/desalting protocol has two distinct advantages over prev

149 Furthermore, several desalting protocols were tested in the development of a

150 the stringent high-resolution and extensive desalting requirements that are essential to the pinpoin

151 s get larger, it becomes more challenging to desalt samples and achieve high-quality intact spectra w

152 applied to samples all at once as opposed to desalting samples one-by-one for 5 min each.

153 phobic interaction chromatography (HIC)) and desalting SEC-MS in the third dimension ((3)D).

154 assay, which included PCA extraction, online desalting, separation of the high-energy phosphates on a

155 a proper pH value and ionic strength of the desalted serum and also utilized Tween 20 to serve as th

156 ed, and the SALDI-MS results obtained on the desalted serum sample spots show both good reproducibili

157 (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified

158 A second desalting step, achieved by dialysis utilizing a membran

159 y using the second-dimension separation as a desalting step.

160 ometric analysis was feasible after a single desalting step.

161 col that requires <1 mug of mAb includes two desalting steps and showed sequence coverages of 95-100%

162 d and require manual fraction collection and desalting steps before analysis through mass spectrometr

163 ptible to nonvolatile salts in solution, and desalting steps might bring undesired consequences: samp

164 The N-glycan modification, digestion, and desalting steps were performed using a single-pot method

165 ant RapiGest (RG), eliminates alkylation and desalting steps, and accomplishes the reduced tryptic di

166 ALDI experiment, without having to resort to desalting steps.

167 substitute for conventional cation-exchange desalting techniques.

168 ocessor for integrated DNA sequencing sample desalting, template removal, preconcentration, and CE an

169 The RPLC also serves to desalt the analytes so that they can be detected in the

170 is system employed an ion suppressor cell to desalt the chromatographic effluent online prior to the

171 irect reversed-phase method to automatically desalt the digestion products and then assessed the desa

172 Carbamylation could be minimized by desalting the cyanylation reaction before cleavage or by

173 separation can be identified by trapping and desalting the fractions onto a series of reversed phase

174 d a porous graphitic carbon (PGC) column for desalting the peptides found in the unretained fraction.

175 hort reversed-phase chromatographic step for desalting the sample, rapid DMS separation of the analyt

176 aloarchaeon Haloferax volcanii that not only desalts the samples thoroughly but also drastically redu

177 With on-chip desalting, the limit of quantitation for histidine spike

178 Following trypsin digestion and desalting, the mitochondrial samples were analyzed by na

179 e conducted and experiments involving sample desalting through solid-phase extraction (SPE) with a re

180 cially available ZipTip C-18 and Aspire RP30 Desalting Tip.

181 However, the former requires desalting to be MS-compatible, and the latter requires f

182 on-exchange (SCX) followed by reversed-phase desalting to remove Ficoll, a synthetic polymer, for HX-

183 These glycopeptides were enriched and desalted using a microscale hydrophilic interaction chro

184 First, a bulk urine pool was desalted using ion-exchange resins enabling large-scale

185 to reaction product peaks were collected and desalted using SPE prior to analysis for the presence of

186 The system also features fast sample loading/desalting using a vented column approach to improve samp

187 Desalted venom had as much anti-M2 as anti-M4 activity.

188 Efficient desalting was demonstrated for both DNA and protein samp

189 On-column enrichment and desalting was demonstrated for large sample volumes (>40

190 oduce ions into (salt) or extract ions from (desalt) water-in-oil droplets.

191 Even after desalting, we show that the choice of matrix still plays

192 enhance mass spectral quality often require desalting, which presents as a bottleneck in matrix-assi

193 rapid digestion, peptide concentration, and desalting while maintaining slow H/D exchange conditions

194 orward workflow without fraction pooling and desalting while showing comparable performance to the ot

195 trap column cleaning and sample loading and desalting with the analysis of the previous sample.