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1 MS analysis of the digestion mixture without desalting.
2 er demanding applications without additional desalting.
3 iring toxic demulsifiers and water-intensive desalting.
4  thin materials for molecular separations or desalting.
5 o those from conventional guanidination with desalting.
6 asured directly from reaction mixtures after desalting.
7  the membrane to small ions allows efficient desalting.
8 bsystems to multiplex sample loading, online desalting, analysis, and column regeneration.
9                                        Rapid desalting and a low volume connection to an electrospray
10 ed using the described protein precipitation/desalting and cation-exchange solid-phase extraction iso
11 ents: (1) isolation by protein precipitation/desalting and cation-exchange solid-phase extraction, (2
12 s were identical regardless of the method of desalting and concentrating of protein samples, samples
13 e for in-well trypsin digestion, followed by desalting and concentrating the digestion products in th
14 ries of sample handling processes, including desalting and concentrating, to be performed directly on
15 ause of the post-electrophoresis extraction, desalting and concentration steps.
16                                              Desalting and concentration takes approximately 5 min wh
17 w effectively eliminated the need for sample desalting and enabled compatible sample processing for m
18 r most molecular chemical techniques, sample desalting and enrichment is a prerequisite.
19 onable analytical procedures with respect to desalting and enrichment were established.
20 To ensure an efficient electrospray process, desalting and exchanging the biomolecular solutions into
21 usion chromatography (muSEC) module for fast desalting and its integration into microfluidics, along
22        All digestates were then combined for desalting and MALDI-TOF/TOF mass spectrometric analysis.
23                   Development of methods for desalting and MS/MS fragmentation allows rapid acquisiti
24 l processing steps on a small scale, such as desalting and protein digestion, could be developed and
25 ked with reversed-phase material for on-chip desalting and reversed-phase chromatography.
26 easurements reveal characteristic regimes in desalting and salting modes and demonstrate that the rat
27                                              Desalting and sample concentration follows each fraction
28 (nRapidFire-MS) capable of performing online desalting and sample introduction in as little as 10 s p
29 ammalian cells, surface adhesion, along with desalting and speed-vacuum drying steps, all contribute
30 applied to tryptic peptide mixtures prior to desalting and spotting onto MALDI-TOF plates.
31 eps involved in a sample preparation such as desalting and various chromatographic purification schem
32 ctions is the time-consuming chromatography, desalting, and concentration steps required to prepare a
33 n steps, including depletion, fractionation, desalting, and concentration.
34 e concentration, buffer exchange, digestion, desalting, and matrix/analyte cocrystallization for MALD
35 ed 16-plex TMT, including labeling reaction, desalting, and MS conditions, and then directly compared
36 buffer exchange, protease digestion, peptide desalting, and, in the case of MALDI-MS, matrix and anal
37 general, and highly efficient, rapid in-line desalting approach using a small gel cartridge to assist
38 rotein A affinity chromatography followed by desalting by gel filtration and was characterized by ami
39                   Following purification and desalting by reversed-phase HPLC, buffer exchange with M
40 o collect CIU fingerprints in 30 s following desalting by using size exclusion chromatography cartrid
41 yzed carbohydrates by replacing ion-exchange desalting cartridges with evaporative removal of HCl und
42 ddition, it provides an effective method for desalting cellular RNA samples having complex matrixes,
43  proteomic analysis through automated sample desalting/cleanup and enrichment of target mass ranges (
44 elds, and product purification using a PD-10 desalting column.
45 I-CIU, among which are (i) improved and fast desalting compared to manual buffer exchange used for cl
46 e antibody-drug conjugate (ADC) using native desalting conditions, we maintain the intact bivalent st
47 isobaric tag labeled glycopeptides after C18 desalting could be readily enriched by SAX and RAX cartr
48 tform for high-throughput and low sample-use desalting coupled with collision-induced dissociation (C
49 ium dodecyl sulfate (SDS) and following with desalting/delipidation of the sample by chloroform/metha
50          We have developed a microfabricated desalting device that meets both requirements, thus prov
51 as been widely applied for concentrating and desalting DOM.
52 atment, commercial LoBind tubes, and in-line desalting during sample processing.
53                                    Two-stage desalting (during solid phase extraction and on the anal
54                                              Desalting efficiency and analyte loss was evaluated with
55                       Importantly, up to 98% desalting efficiency is achieved.
56 roximately 80% and demonstrate the exquisite desalting efficiency with high-performance electrospray
57        This approach allows effective sample desalting, enrichment, sequential elution, and MS detect
58 ving as proof-of-concept, demonstrate alphaS desalting, exchange buffer efficiency, and reduced solve
59 ne protein targets, we show that appropriate desalting followed by different folding strategies enabl
60 he procedure does not require alkylation and desalting, greatly shortening the sample preparation tim
61                                              Desalting is not needed prior to MALDI-TOF MS.
62 edures are detrimental to MALDI-MS, and thus desalting is required before the digestion products can
63                                         This desalting method directly separates highly polar, ionic
64     This method provides a simple, versatile desalting method for measuring exometabolites of environ
65  MALDI-TOF, applying our successive on-plate desalting method that eliminates the insensitivity of th
66 uid chromatography-mass spectrometry (LC-MS) desalting method that mitigates these shortcomings.
67 sample preparation is improved using a novel desalting method that utilizes the hydrophobic surface o
68 ca columns; however, these entail subsequent desalting methodologies and consequent sample losses and
69                              The most common desalting methods are faced with major limitations, incl
70 versed-phase liquid chromatography (IP-RPLC) desalting methods we previously employed do not effectiv
71           Its removal by various solid-phase desalting methods, catalase treatment, or freeze drying
72                      We evaluated a range of desalting methods, including spin columns, dialysis memb
73 IMAC procedure was significantly improved by desalting methylated peptides, followed by gradient elut
74                             Specifically for desalting NaCl, this enhancement of unipolar cation cond
75 In this study, we present a novel method for desalting of ADCs and mAbs building on the SP3 approach
76       Overall, the (2)D HILIC allowed online desalting of AEX and IPRP modes and further separation o
77 assess the feasibility of this approach, the desalting of alpha-synuclein (alphaS) was investigated u
78  octadecyl amine (ODA) to be employed in the desalting of complex mixtures and the results are compar
79                   This protocol involves the desalting of nucleic acids using ammonium acetate precip
80                                              Desalting of protein solutions is demonstrated for ESI-M
81                  Elimination of the need for desalting of samples after reaction raised the possibili
82                                 The complete desalting of samples, which is necessary for IEF, tends
83                Size-selective separation and desalting of small model molecules ( approximately 200-1
84 graphic media for the microconcentration and desalting of SUPREX samples.
85 phase purification approach also facilitates desalting of the captured oligonucleotides, which is ess
86 of salt adducts without the need for offline desalting or buffer exchange, and each multiplexed measu
87  spin-type kits are extensively used for the desalting or buffer-exchange of mAbs and ADCs samples.
88 gonal separation methods that do not require desalting or extensive sample handling.
89 ut the need for intermediate chromatography, desalting, or concentration steps.
90 e presence of excess MnCl2 followed by rapid desalting over a gel filtration column resulted in the r
91 n nonlinear increases in energy required for desalting over standard IEM devices due to electroosmoti
92  nucleic acids, we have developed a nanoflow desalting platform for high-throughput and low sample-us
93                                       Online desalting platforms have enabled facile sample cleanup a
94  the molecular ion region of the analytes, a desalting procedure of the MBM sample directly on the MA
95 reby eliminating conventional time-consuming desalting procedures required for downstream analysis of
96  to be applied in field studies, eliminating desalting procedures, which can be time-consuming and no
97 anes; the first one with MWCO below 5700 for desalting protein samples, and the second one with a hig
98  attractive alternative to other methods for desalting proteins prior to mass spectrometry analysis.
99                  This tip-based purification/desalting protocol has two distinct advantages over prev
100                         Furthermore, several desalting protocols were tested in the development of a
101  the stringent high-resolution and extensive desalting requirements that are essential to the pinpoin
102 applied to samples all at once as opposed to desalting samples one-by-one for 5 min each.
103 phobic interaction chromatography (HIC)) and desalting SEC-MS in the third dimension ((3)D).
104 assay, which included PCA extraction, online desalting, separation of the high-energy phosphates on a
105  (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified
106                                     A second desalting step, achieved by dialysis utilizing a membran
107 y using the second-dimension separation as a desalting step.
108 ometric analysis was feasible after a single desalting step.
109 col that requires <1 mug of mAb includes two desalting steps and showed sequence coverages of 95-100%
110 d and require manual fraction collection and desalting steps before analysis through mass spectrometr
111 ptible to nonvolatile salts in solution, and desalting steps might bring undesired consequences: samp
112    The N-glycan modification, digestion, and desalting steps were performed using a single-pot method
113 ant RapiGest (RG), eliminates alkylation and desalting steps, and accomplishes the reduced tryptic di
114 ALDI experiment, without having to resort to desalting steps.
115  substitute for conventional cation-exchange desalting techniques.
116 ocessor for integrated DNA sequencing sample desalting, template removal, preconcentration, and CE an
117          Carbamylation could be minimized by desalting the cyanylation reaction before cleavage or by
118 separation can be identified by trapping and desalting the fractions onto a series of reversed phase
119 d a porous graphitic carbon (PGC) column for desalting the peptides found in the unretained fraction.
120 hort reversed-phase chromatographic step for desalting the sample, rapid DMS separation of the analyt
121                                 With on-chip desalting, the limit of quantitation for histidine spike
122              Following trypsin digestion and desalting, the mitochondrial samples were analyzed by na
123 e conducted and experiments involving sample desalting through solid-phase extraction (SPE) with a re
124 cially available ZipTip C-18 and Aspire RP30 Desalting Tip.
125                 However, the former requires desalting to be MS-compatible, and the latter requires f
126 on-exchange (SCX) followed by reversed-phase desalting to remove Ficoll, a synthetic polymer, for HX-
127 The system also features fast sample loading/desalting using a vented column approach to improve samp
128                                    Efficient desalting was demonstrated for both DNA and protein samp
129                     On-column enrichment and desalting was demonstrated for large sample volumes (>40
130                                   Even after desalting, we show that the choice of matrix still plays
131  enhance mass spectral quality often require desalting, which presents as a bottleneck in matrix-assi
132  rapid digestion, peptide concentration, and desalting while maintaining slow H/D exchange conditions
133 orward workflow without fraction pooling and desalting while showing comparable performance to the ot
134  trap column cleaning and sample loading and desalting with the analysis of the previous sample.

 
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