ライフサイエンスコーパス: detergent

1 d to 97% when the samples were washed with a detergent.

2 peratures or pH values and the presence of a detergent.

3 d to an effect expected from the action of a detergent.

4 its loss can be minimized by the addition of detergent.

5 h-resolution fractionation in the absence of detergent.

6 cultures, solubilized and purified in Facade detergent.

7 h spike protein formulated in polysorbate 80 detergent.

8 otease are subject to constant exchange with detergent.

9 rom a single-chain galactolipid and nonionic detergents.

10 ScBOR1p isolates as a monomer in a range of detergents.

11 as solubilized by non-ionic and zwitterionic detergents.

12 tial solubilization condition into different detergents.

13 , ease of purification, and functionality in detergents.

14 OWCs) such as pharmaceuticals, hormones, and detergents.

15 s in a changed diet, and chemicals in modern detergents.

16 turally different from those crystallized in detergents.

17 derived from genotype 1b (isolate J4) in the detergent 6-cyclohexyl-1-hexylphosphocholine (Cyclofos-6

18 of engineered receptors and the presence of detergents(7-9).

19 s their extraction from native sources using detergents, a step that can lead, possibly irreversibly,

20 y proceeds in the absence of dialysis and/or detergent absorbents, and A2AR assimilation into synthet

21 Collectively, our results demonstrate that detergents affect the solubility of individual proteins,

22 at 65 degrees C in a higher concentration of detergent and a minimized sonication step, to produce ro

23 ep stable in a high concentration of anionic detergent and exhibit synergistic anti-cancer efficacy.

24 and Ca(2+)-free conformations of nhTMEM16 in detergent and lipid nanodiscs illustrate the interaction

25 tern of mobilities and spectral features, in detergent and liposomes, for residues at the pore domain

26 density, resistance to being solubilized by detergent and quenching of fluorophores within the vesic

27 l as seven other common chemicals, including detergents and fixatives.

28 studied by cross-linking in the presence of detergents and lipids.

29 nts we crystallized LL-37 in the presence of detergents and obtained the structure of a narrow tetram

30 When detergents and phospholipid membranes are dispersed in a

31 otein complex, a membrane protein complex in detergent, and lipoprotein nanodiscs with and without em

32 a permeability barrier against antibiotics, detergents, and environmental stresses.

33 rom a variety of contaminants such as salts, detergents, and heavy metal components using solar energ

34 nts is more restricted than in shorter chain detergents, and maltoside micelles are more restricted t

35 ectly linked these functional differences in detergent- and HDL-reconstituted beta(2)AR to a change i

36 backbone assignments of membrane proteins in detergents are available, they are largely absent for me

37 w that, despite the conventional wisdom that detergents are deleterious to mass spectrometric analyse

38 addressed to the membrane and extractable by detergents are generally assumed to be properly folded.

39 Detergents are usually used to extract these bio-macromo

40 Detergents are widely used in modern in vitro biochemist

41 s that may correspond to ergosterol or bound detergent, around the c-ring.

42 cs as a potentially universal alternative to detergents as a means to stabilize membrane proteins in

43 rrel with 283 residues, for which 67% of the detergent assignment could be transferred to the nanodis

44 es of NPC1 were obtained in nanodiscs and in detergent at resolutions of 3.6 angstrom and 3.0 angstro

45 either a conformational change or binding of detergent at the binding site in a detergent micelle env

46 unknown, and its analysis largely relies on detergent-based preparations devoid of endogenous ligand

47 A related, detergent-based protocol for scrambling the lipid asymme

48 eractions are largely undetected by standard detergent-based purification.

49 Here, we optimized a detergent-based reconstitution protocol to develop a pro

50 rotein crystallization, and to visualize the detergent belt for Cryo-EM studies.

51 retinal from Rho in an in vitro phospholipid/detergent bicelle system.

52 he dissociated from the intact oligomers and detergent-bound complexes and correlate the reported cyt

53 o validate the interactions seen in previous detergent-bound structures.

54 en without a target; therefore, a denaturing detergent buffer is necessary for exhaustive extraction

55 membrane proteins studies require the use of detergents, but because of the lack of a general, accura

56 ysiological conditions and in the absence of detergents, but traditional styrene-maleic acid copolyme

57 endent protein interaction networks, because detergents can simultaneously disrupt the very interacti

58 Here, we characterize the effect of three detergents commonly used to study synaptic proteins on a

59 n CB(2) thermostability caused by mutations, detergent composition, and the presence of stabilizing l

60 ecellularisation protocol incorporated a low detergent concentration and hypotonic buffers.

61 near peak shift trajectories with increasing detergent concentration.

62 In contrast, at detergent concentrations comparable with or below the CM

63 ng and activity-based assays under optimized detergent conditions can support selection of thermostab

64 oughput screening method to identify optimal detergent conditions for membrane protein stabilization.

65 fractory to coimmunoprecipitation under mild-detergent conditions.

66 show how to reliably and easily estimate the detergent corona diameter and select the smallest size,

67 ences in membrane protein stability in these detergents could be due to fluidity in addition to the a

68 only occurred for membrane proteins and was detergent-dependent, being most pronounced in long polye

69 R-like requires also the use of LMNG, a mild detergent developed for crystallography to increase memb

70 tent across detergent types, suggesting that detergents do not isolate distinct protein pools with un

71 A unique combination of a nonionic detergent dodecyl-beta-d-maltopyranoside (DDM) with urea

72 hanges is more or less feasible in different detergents due to baseline solubility.

73 re superficial, supporting the notion that a detergent effect underlies its hemolytic activity.

74 n proposed: the pore formation model and the detergent effect.

75 Detergents enable the purification of membrane proteins

76 e II cannabinoid receptor CB(2), in a Facade detergent enables radioligand thermostability assessment

77 lpha3beta4 nicotinic receptor in lipidic and detergent environments, using functional reconstitution

78 The procedure requires that lipid-detergent exchange kinetics are in the fast exchange reg

79 ation following infection, oxidative stress, detergent exposure and wounding.

80 ng, and for ISC activation upon infection or detergent exposure.

81 y have permanent disability owing to laundry detergent exposure; and concerted intervention is needed

82 Detergent-extracted fiber bundles revealed a significant

83 rogroup B vaccines, including one containing detergent-extracted OMV, did not produce gonococcal SBA

84 unoprecipitation-Western blotting using high-detergent extracts revealed a variety of SDS-stable low-

85 Low-detergent extracts tested by 82E1 enzyme-linked immunoso

86 ct that membrane proteins retain activity in detergent extracts) that phospholipid environment is a s

87 , as DM litter had higher initial N, neutral detergent fiber (NDF) solubles and holocellulose:lignin

88 reflects issues with the traditional use of detergents for extraction, as the surrounding lipids are

89 dentify guidelines that allow fine-tuning of detergents for individual applications in membrane prote

90 s novel opportunities for the application of detergents for the investigation of membrane proteins.

91 iously reported using the QTY code to design detergent-free chemokine receptors.

92 We here report the design of 2 detergent-free chimeric chemokine receptors that were ex

93 stery and drug binding in a more natural and detergent-free lipid bilayer.

94 ractive tool to study membrane proteins in a detergent-free lipid-bilayer environment.

95 ree expression technologies, even completely detergent-free membrane protein characterization protoco

96 speeds up gradually from taking weeks in the detergent-free membrane to minutes or less in the leakin

97 styrene maleic acid (SMA) copolymers offer a detergent-free method for biological membrane solubilisa

98 zation (DM-PSI) and a, to our knowledge, new detergent-free method using styrene-maleic acid copolyme

99 t amphipathic or hydrophobic substrates in a detergent-free native or artificial membrane environment

100 were conducted using purified DGKepsilon and detergent-free phospholipid aggregates, which present a

101 le tool for high-yield production of intact, detergent-free prions that retain in vivo activity.

102 ch enables affordable scale-up production of detergent-free QTY variant chemokine receptors with tuna

103 We also show that all detergent-free QTY-designed chemokine receptors, express

104 Quite surprisingly, the detergent-free SMA-PSI complexes upon excitation by thes

105 generates transcriptomes and proteomes from detergent-free tissue lysates fractionated across a sucr

106 cs of phosphocholine and maltoside micelles, detergents frequently used for membrane protein structur

107 plex, where the lipid cannot be displaced by detergent from the highly protected active site.

108 py, kinetically resolves the dissociation of detergents from membrane proteins and protein unfolding.

109 Deoxycholic acid, a strong detergent, greatly enhanced the conjugation yield likely

110 Even though a large variety of detergents have been developed in the last century, the

111 f membrane proteins have been obtained using detergents; however, these can promote local lipid rearr

112 We hypothesize that the DDM detergent improves protein recovery by efficiently reduc

113 ysosomal permeability through a lysomotropic detergent in cells devoid of Bax/Bak1 restores autophagi

114 Addition of detergent in milk can cause food poisoning and other com

115 usly attributed to ESAT-6 is due to residual detergent in the preparations.

116 that allows quantification of pure or mixed detergents in complex with membrane proteins.

117 fically, phosphocholines are frequently used detergents in NMR studies, and maltosides are frequently

118 sistance to heat (32 degrees C) and specific detergents in the insect pathogenic fungus, Beauveria ba

119 The benefits of inclusion of detergents in the LESA sampling solvent are also demonst

120 Detergent-induced lipid scrambling of liposomes mimickin

121 s been successfully exploited in textile and detergent industries.

122 aeruginosa infection elicits accumulation of detergent insoluble tau protein in the mouse brain and i

123 we report that Akita mutant proinsulin forms detergent-insoluble aggregates that entrap wild-type (WT

124 rotein misfolding and not to the presence of detergent-insoluble aggregates.

125 e found to have increased phosphorylated and detergent-insoluble alpha-synuclein deposits.

126 Notably, detergent-insoluble ferritin accumulates in RPE cells an

127 Wild-type UL148 accumulates in a detergent-insoluble form during infection.

128 an integral membrane protein that resides in detergent-insoluble membrane fractions enriched in conde

129 (PD), such as demonstrating the presence of detergent-insoluble membrane microdomains enriched in st

130 g events and involved EGFR clustering within detergent-insoluble plasma mebrane-associated tubules.

131 transgenic worms, reduced phosphorylated and detergent-insoluble tau accumulation, and reduced tau-me

132 gnostic of CBD(7,8); by SDS-PAGE, so too are detergent-insoluble, 37 kDa fragments of tau(9).

133 striatin that forms stable associations with detergent-insoluble, membrane-bound cellular fractions.

134 ~5 min) unfolds and self-associates, forming detergent-insoluble, microscopic cytoplasmic aggregates.

135 Furthermore, this detergent is compatible with a [(35)S]GTPgammaS radionuc

136 roteins solubilized from cell membranes into detergents is a challenging task.

137 ure medium showed that virus inactivation by detergents is annulled at physiological serum concentrat

138 The solubilization of membranes by detergents is critical for many technological applicatio

139 de probe rotational dynamics in longer chain detergents is more restricted than in shorter chain dete

140 le of individual BA species as physiological detergents is relatively ubiquitous, their endocrine fun

141 equire artificially low expression levels or detergent isolation to achieve the low fluorophore conce

142 in the SMA-PSI that may be disrupted during detergent isolation.

143 SpNOX was solubilized in the detergent lauryl maltose neopentyl glycol, which provide

144 on of active transporters, whereas a harsher detergent like Fos-choline 12 could solubilize transport

145 Polymyxins are a group of detergent-like antimicrobial peptides that are the ultim

146 In contrast, this detergent-like effect was not observed for Abeta monomer

147 hat hGBP1 binds directly to LPS and induces "detergent-like" LPS clustering through protein polymeriz

148 nges pertinent to identification of an ideal detergent, lipid, or detergent/lipid mixture that closel

149 ique tool to unravel the complex kinetics of detergent-lipid interactions.

150 ntification of an ideal detergent, lipid, or detergent/lipid mixture that closely mimic their native

151 of industrial products, from biomedicines to detergents, lubricants, and coatings.

152 Use of the MESMER assay versus a comparable detergent lysis-based assay, cellular Fura-2 Mn extracti

153 r, we observed a general trend in which mild detergents mainly extract the population of active trans

154 further show that fos-choline and PEG family detergents may lead to membrane protein destabilization

155 ptimization of desired lipid composition, 2) detergent-mediated protein reconstitution with subsequen

156 SDS and has minimal interference from other detergents, metals, and inorganic ions.

157 ts (i.e., from Qiagen) and a simple heat and detergent method that extracts viral RNA directly off th

158 inding of detergent at the binding site in a detergent micelle environment.

159 e scattering contribution of the surrounding detergent micelle rendered invisible.

160 ve IM-MS without the need to fully strip the detergent micelle, which can cause significant gas-phase

161 We also analyze intact detergent micelle-embedded alphaHL porelike complexes by

162 ich consist of two FtsH hexamers in a single detergent micelle.

163 ated rhomboid proteases, can be used both in detergent micelles and in liposomes, and contain red-shi

164 nces between LRRC8A structures determined in detergent micelles and lipid bilayers related to reorgan

165 erfacial activation as well as inhibition by detergent micelles and lipoprotein particles.

166 (D)'s and cooperativity are observed between detergent micelles and proteoliposomes, the physiologica

167 have been investigated in lipid bilayers and detergent micelles by solution NMR relaxation techniques

168 We show that a resonance assignment in detergent micelles can be transferred to a spectrum reco

169 reased stability, they are often superior to detergent micelles or liposomes for membrane protein sol

170 lls along the way, largely due to the use of detergent micelles to protect and stabilize complexes.

171 racellular loop 3 (ICL3) was crystallized in detergent micelles using vapor-phase diffusion.

172 ical properties of P-gp in native membranes, detergent micelles, and when reconstituted in artificial

173 However, at higher concentrations, detergent micelles, latex nanobeads or lipoprotein parti

174 surfaces of hydrophobic aggregates, such as detergent micelles, lipoprotein particles and even polys

175 nt of a membrane protein can be conducted in detergent micelles, opening the possibility for the dete

176 ing crystal structures of MdfA, show that in detergent micelles, the protein adopts a predominantly o

177 However, conventional native MS relies on detergent micelles, which may disrupt natural interactio

178 BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells,

179 nts with purified receptors reconstituted in detergent micelles.

180 cies with "native states" mostly obtained in detergent micelles.

181 ptides form coclusters with membrane mimetic detergent micelles.

182 ement, both in native membranes and in mixed detergent micelles.

183 ia contacts between proteins and mixed lipid/detergent micelles.

184 ations for stabilization of purified GPCR in detergent micelles.

185 significant effects on the thermodynamics of detergent micellization.

186 ology of ideal bicelles even at low lipid-to-detergent mole ratios.

187 sferred into the mass spectrometer where the detergent molecules are stripped away using collisional

188 The channel's periplasmic exit is sealed by detergent molecules that block solvent permeation.

189 oncerning the different components (protein, detergent molecules) of detergent-solubilized membrane p

190 ain oligomeric state-proportional numbers of detergent molecules.

191 A lysosomotropic detergent (MSDH) and an autophagy inhibitor (Lys05) are

192 eatment and solubilization with the nonionic detergent n-dodecyl-beta-d-maltoside, which preserved bo

193 When mixed with the classical detergent n-dodecylmaltoside (DDM), the four hybrids wer

194 ere we introduce the family of oligoglycerol detergents (OGDs).

195 ifferent experimental results, the effect of detergent on activity-dependent synaptic protein complex

196 n apprehension of the adverse effect of such detergents on various living organisms.

197 active pharmaceutical ingredients (APIs) and detergents onsite.

198 Protein stability in detergent or membrane-like environments is the bottlenec

199 mainly exists in solutions as complexes with detergents or lipoprotein particles.

200 nt on many factors such as concentrations of detergents or lipoproteins, incubation time, as well as

201 ems, we developed a method that does not use detergents or other chemicals.

202 describe the interactions with cholesterol, detergents, peptides, and integral membrane proteins and

203 nt of cMyBP-C N'-terminal domains (C0-C7) in detergent-permeabilized cardiomyocytes from gene-edited

204 the specimens were perfused using a combined detergent/polar solvent decellularization protocol.

205 ved with the inclusion of 0.002% of the mild detergent polysorbate 80 in the MIC assay.

206 receptor core to interact with the lipid or detergent, providing an explanation for the distinct act

207 We reconstituted detergent-purified mitochondrial ATP synthase dimers fro

208 Leveraging the benefits of an acid-cleavable detergent, RapiGest SF Surfactant (Waters Corporation),

209 Compared with detergent-reconstituted beta(2)AR, the beta(2)AR in HDL

210 al and two alternative methods for efficient detergent removal to enable quantitative proteomic analy

211 iated protein reconstitution with subsequent detergent removal, 3) generation of lipid asymmetry by p

212 ng other buffer formulations with or without detergent removal.

213 greatly enhance TDP-43 aggregation, forming detergent-resistant and hyperphosphorylated inclusions.

214 scopy revealed that betaIII spectrin forms a detergent-resistant cytoskeletal network at these sites.

215 By contrast, detergent-resistant lipids bound at the dimer interface

216 talizes exclusively to caveolin-1-associated detergent-resistant membrane (DRM) vesicles in HT-29 cel

217 y accumulates in intracellular Xfect-induced detergent-resistant membrane compartments which appear t

218 rain failed to reduce CD40 relocation to the detergent-resistant membrane domain and to inhibit CD40-

219 luster PM-derived cholesterol into transient detergent-resistant membrane domains (DRMs) within the E

220 nt differences in the protein composition of detergent-resistant membrane fractions from wildtype and

221 C162A modulated sodium current and sorted to detergent-resistant membrane fractions normally.

222 ) to the virus assembly sites located at the detergent-resistant membranes (DRM).

223 liquid phase separation and detected similar detergent-resistant oligomers upon maturation of liquid

224 tes from being predominantly nuclear to form detergent-resistant, hyperphosphorylated aggregates in t

225 mg fibers/g textile washed, without and with detergent, respectively), the overall microplastic fiber

226 NaOH, found in table salt, baking soda, and detergents, respectively.

227 ins were used as test cases to benchmark our detergent screening method.

228 tion of protein immobilization by denaturing detergents (SDS) and incubation at elevated temperatures

229 ady-state and dynamic contractile indices in detergent-skinned guinea pig (Cavia porcellus) cardiac m

230 in humans reported higher deposition of the detergent sodium lauryl sulphate in those exposed to har

231 or membrane protein digestion using an ionic detergent, sodium dodecyl sulfate (SDS), at high tempera

232 integral membrane transport protein in both detergent-solubilised micelles and reconstituted proteol

233 stratified these proteins according to their detergent solubility profiles.

234 the method to study the structure of an IMP, detergent solubilization from the membrane is usually th

235 Unexpectedly, we observed that detergent solubilization induces the formation of fully

236 of multiple copper binding sites, effects of detergent solubilization on activity and crystal structu

237 eriments with membrane proteins are based on detergent solubilization.

238 The detergent-solubilized complex adopts a three-bladed prop

239 olution structures of engineered soluble and detergent-solubilized Env trimers.

240 ssess the formation and thermostabilities of detergent-solubilized fluorescent protein-tagged CLR.RAM

241 d that GGPP-regulated misfolding occurred in detergent-solubilized Hmg2, a feature that will allow ne

242 Analysis of detergent-solubilized Hrd1.KI cells indicates that the c

243 components (protein, detergent molecules) of detergent-solubilized membrane protein complexes.

244 dvantageous approach for charge reduction of detergent-solubilized membrane proteins by native MS.

245 o characterize the solution structure of the detergent-solubilized multidrug transporter MdfA from E.

246 All previous studies on human SQR have used detergent-solubilized protein.

247 Crystal structures of detergent-solubilized rat TRPV6 in the closed state have

248 rmational rearrangement was observed both in detergent-solubilized SthK and in channels reconstituted

249 er membranes, but they do not predominate in detergent-solubilized VDAC samples.

250 (CHS), was shown to induce the formation of detergent-soluble VDAC multimers.

251 Vimentin collapse correlated with a loss of detergent-soluble vimentin filament precursors and decre

252 and -D288R proved to be mostly monomeric in detergent solution and after reconstitution into proteol

253 tein fluorescence quenching, bound Fe(2+) in detergent solution with low micromolar affinity.

254 ors that were experimentally unattainable in detergent solution.

255 oach to obtain monomeric fluorescent CCR5 in detergent solution.

256 )E(4)) and tetradecylphosphocholine (FOS-14) detergent solutions.

257 size exclusion chromatography, although the detergent-specific abundance of proteins in high molecul

258 d GT) methods to determine charge states and detergent stoichiometry distributions.

259 tants had similar sensitivity to osmotic and detergent stress and lipopolysaccharide profile and an i

260 hibited increased sensitivity to osmotic and detergent stress, lacked very long lipopolysaccharide, w

261 nced in long polyethylene glycol (PEG)-based detergents such as C10E5 and C12E8.

262 temperature in the presence of strong ionic detergents such as SDS.

263 their portals and could be disaggregated by detergents, supporting a role for membranes in their for

264 embrane proteins solubilized in conventional detergents tend to undergo structural degradation, neces

265 e nanodiscs without exposure to conventional detergents that destabilize protein structures and induc

266 In the presence of detergent, the C protein is retained on purified ribonuc

267 on between HCPs and antibody with an anionic detergent, the depletion of antibody from HCPs can be ea

268 rred to a spectrum recorded in nanodiscs via detergent titration.

269 Here we used calixarene detergent to solubilize and purify wild-type non-aggrega

270 ignificant because natural receptors require detergents to become soluble.

271 inding properties, we explore the ability of detergents to compete with lipids bound in different env

272 The use of detergents to isolate the PSD and release its membrane-a

273 s lagged, partly due to the necessary use of detergents to maintain protein solubility.

274 ane vesicle (OMV) vaccines are prepared with detergents to remove endotoxin, which also remove desira

275 e introduced peptidiscs as an alternative to detergents to stabilize membrane proteins in solution (C

276 stance to membrane-attacking antibiotics and detergents to which E. coli would usually be considered

277 rth, birth order, increased use of soaps and detergents, tobacco smoke exposure and psychosomatic fac

278 ctors such as protease enzymes of allergens, detergents, tobacco, ozone, particulate matter, diesel e

279 interacts with the G9/A16 EFC subcomplex in detergent-treated cell extracts.

280 hough some association was still detected in detergent-treated infected cell lysates.IMPORTANCE The e

281 etically attenuated endotoxin do not require detergent treatment and elicit broader serum bactericida

282 However, 0.1% detergent treatment did not inactivate EBOV in blood sam

283 the exoskeleton construction was followed by detergent treatment to permeabilize the cytoplasmic memb

284 , CbpD, and CibAB and is more susceptible to detergent-triggered lysis.

285 Real-time injection of a non-ionic detergent, Triton X, induced biphasic solubilization kin

286 Incubation at 80 degrees C, a range of detergents, Trizol reagents, and UV energies were succes

287 in protein complexes were consistent across detergent types, suggesting that detergents do not isola

288 ions, when detectable, are consistent across detergent types.

289 -dodecyl-beta-d-maltoside, a micelle-forming detergent, we are able to discern the dissociated from t

290 rotein solubilized in "match-out" deuterated detergent, we have been able to interrogate a "naked" HT

291 After extraction with non-denaturing detergents, we affinity-purified 785 endogenously tagged

292 Moreover, given the modular design of these detergents, we anticipate fine-tuning of their propertie

293 Second, experiments have been performed in detergent, which can induce non-native conformations, or

294 Furthermore, detergents, which are often used to solubilize membrane

295 to Octyl-Sepharose and could be released by detergent, while uncleaved proheads without portal or cl

296 We were able to identify groups of detergents with characteristic stabilization and destabi

297 analyzing trace samples of API and cleaning detergents with various substrates.

298 Reaction of the purified protein, in detergent, with the thiol-reactive N-ethylmalemide (NEM)

299 mokine receptors in aqueous solution without detergent would be significant because natural receptors

300 toxic substances, including antibiotics and detergents, yet allows acquisition of nutrients necessar

301 espite widespread recognition that different detergents yield different experimental results, the eff