ライフサイエンスコーパス: diaphanous

1 on in a human homolog of the Drosophila gene diaphanous.

2 on of the actin assembly factors Enabled and Diaphanous.

3 nd promoted junction integrity by activating Diaphanous.

4 malian formins, inverted formin 2 (INF2) and diaphanous 1 (DIA1, encoded by DIAPH1), exemplify this d

5 The diaphanous-related formin, Diaphanous 1 (DIAPH1), is required for the assembly of F

6 n polymerization induced by the mouse formin Diaphanous 1 (mDia1).

7 E with the intracellular effector, mammalian diaphanous 1 or DIAPH1.

8 ing the DIAPH1 gene, which encodes the human Diaphanous 1 protein (hDia1).

9 h the effector protein, mDia1 (for mammalian Diaphanous 1).

10 Loss of the formin mammalian Diaphanous 1, a regulator of linear actin polymerization

11 -related gene in leukocytes alpha) and mouse diaphanous 1, autoinhibition regulates a novel membrane

12 t the RAGE cytoplasmic domain interacts with Diaphanous-1 (Dia-1) both in vitro and in vivo.

13 Conversely, the actin-nucleating formins, Diaphanous-1 (DIA1) and Formin-like-1 (FMNL1), did not a

14 c tail of RAGE (ctRAGE) binds to the formin, Diaphanous-1 (DIAPH1), and this interaction is important

15 We tested the hypothesis that the formin Diaphanous-1 (DIAPH1), which regulates actin dynamics, s

16 xes between the F-actin polymerizing protein Diaphanous-1) and the mitochondrial dynamics protein Mit

17 lopodia formation via the Arp2/3 complex and Diaphanous 2 (Diaph2).

18 ubules, the activation of RhoA-GTP-dependent diaphanous 2 was observed, with no significant activatio

19 tidylinositol 3-kinase (PI3-K), Rho-GTPases, Diaphanous-2 (Dia-2), Ezrin, protein kinase C-zeta, extr

20 hogenesis acting during migratory stages and Diaphanous acting during postmigratory stages.

21 adhesion, suggesting a common mechanism for Diaphanous action during morphogenesis.

22 e hypothesized that differing Enabled and/or Diaphanous activity drives these differences.

23 rocesses, we generated embryos deficient for Diaphanous and analyzed three cell-cycle-regulated actin

24 The actin elongation factors Diaphanous and Enabled both promote barbed-end actin pol

25 n with quantitative approaches revealed that Diaphanous and Enabled each regulate filopodial behavior

26 es on the regulated cortical localization of Diaphanous and Enabled to produce actin cable arrays wit

27 ent of the septin Peanut and distribution of Diaphanous and F-actin at furrows.

28 FHOS has sequence homology to Diaphanous and Formin proteins within the Formin Homolog

29 in furrow-initiation mutants of RhoGEF2 and Diaphanous and in furrow-progression mutants of Anillin.

30 w that this actomyosin flow was regulated by Diaphanous and ROCK and that it elicited a wave of apica

31 -actin, the centralspindlin complex, formin (diaphanous), and profilin (chickadee) are required to st

32 lgi apparatus with the formin-family protein Diaphanous, and loss of either isoform perturbs cell cyc

33 ization of the Arp2/3 complex and the formin Diaphanous, and mutations in diaphanous or capping prote

34 inhibition depends upon the presence of the diaphanous auto-regulatory domain (DAD) C-terminal to FH

35 xamine previously identified coiled-coil and Diaphanous auto-regulatory domain sequences.

36 RMT7 methylates two arginine residues in the diaphanous autoinhibitory domain (DAD) of the family of

37 nhibited through intramolecular binding of a Diaphanous autoinhibitory domain (DAD) to a conserved N-

38 c "RRKR" motif of its autoregulatory domain, diaphanous autoinhibitory domain (DAD), are implicated i

39 a role in nucleation was identified for the Diaphanous autoinhibitory domain (DAD), which is C-termi

40 GTPase binding domain (GBD) and a C-terminal Diaphanous autoinhibitory domain (DAD, ).

41 ition, INF2 binds actin monomers through its diaphanous autoregulatory domain (DAD) that resembles a

42 itory domain (DID) and the carboxyl-terminal diaphanous autoregulatory domain (DAD).

43 ous inhibitory domain (DID) and a C-terminal diaphanous autoregulatory domain (DAD).

44 yl terminus of Bni1 that are adjacent to its diaphanous autoregulatory domain (DAD).

45 s inhibitory domain (DID) and its C-terminal diaphanous autoregulatory domain (DAD).

46 anous inhibitory domain (DID) and C-terminal diaphanous autoregulatory domain (DAD).

47 d well-studied motif is the ~15-residue-long diaphanous autoregulatory domain.

48 predicting partial truncation of the DIAPH1 diaphanous autoregulatory domain.

49 ding of a Diaphanous inhibitory domain and a Diaphanous autoregulatory domain.

50 Recently, the C-terminal diaphanous-autoregulatory domain (DAD) and the C terminu

51 se-binding domain (mDiaN) and the C-terminal Diaphanous-autoregulatory domain (DAD).

52 d Cdc42, or the regulatory formins dDAAM and Diaphanous caused mislocalization of Zipper and induced

53 Perturbation of Diaphanous causes increasing defects in apical nuclear m

54 interacting with microfilaments, chickadee, diaphanous, Cdc42, quail, spaghetti-squash, zipper, and

55 We characterized Diaphanous (Dia) and Enabled (Ena) as a model, using com

56 Furthermore, we show that the formin Diaphanous (DIA) functions with APC2 in this process.

57 the actin caps to be a composite material of Diaphanous (Dia)-based actin bundles interspersed with i

58 Diaphanous (Dia)-related formins (DRFs) coordinate cytos

59 phila leading-edge (LE) cells to explore how Diaphanous (Dia)-related formins and Ena/VASP proteins c

60 lex, is also capable of interacting with the Diaphanous (Dia)-related formins in the absence of Wave.

61 ables generated by the formin-family protein Diaphanous (Dia).

62 Diaphanous (DIAPH) three (DIAPH3) is a member of the for

63 To gain insight into the function of Diaphanous during cytokinesis and explore its role in ot

64 deriving new insights into (a) the roles of Diaphanous, Enabled, and Capping protein in regulating f

65 A novel member of the Formin/Diaphanous family of proteins was cloned and characteriz

66 icellular actomyosin cables, which depend on Diaphanous for their nucleation.

67 ntractile ring components, the Rho effectors diaphanous formin and myosin-II.

68 lymerization that involves activation of the Diaphanous formin Dia.

69 Formin1 (FMN1), a member of the non-diaphanous formin family, is essential for development a

70 mDia1 is a member of the Diaphanous formin subfamily (Dia), whose members contain

71 inhibit actin nucleation and bundling by the diaphanous formin-2 (mDia2) - an actin nucleator that is

72 is upstream of both myosin-II activation and diaphanous formin-mediated filamentous actin (f-actin) a

73 ical progenitors, unravel novel functions of Diaphanous formins and add insights into the pathobiolog

74 in tubular organs of Drosophila, the role of Diaphanous formins at the final stages of secretion appe

75 A subset of formins termed "diaphanous formins" are regulated by autoinhibition thro

76 nduced cellular activation, sequestration of Diaphanous formins, and clustering of the receptor.

77 Diaphanous formins, including mDia1, mDia2, and mDia3 in

78 ght on regulatory mechanisms specific to non-diaphanous formins.

79 We tested this hypothesis by reducing Diaphanous function, revealing striking roles in stabili

80 The diaphanous gene is the founding member of a family of Di

81 We found that Diaphanous has a dynamic expression pattern consistent w

82 Our findings thus indicate that Diaphanous has a role in actin cytoskeleton organization

83 The Drosophila Formin Homology (FH) protein Diaphanous has an essential role during cytokinesis.

84 The genes that have been identified encode diaphanous (HDIA1), alpha-tectorin (TECTA), the transcri

85 ns during cell shape change, suggesting that Diaphanous helps coordinate adhesion and contractility o

86 activating RhoA and the downstream effector Diaphanous homolog 1 (Dia1).

87 gnaling, we investigated the role of protein diaphanous homolog 1 (known as Diaph1 or mDia1) for the

88 1) confirmed rs318125, downstream of DIAPH2 (diaphanous homolog 2 (Drosophila)) (Pallele=0.010, Pgeno

89 We have identified Diaphanous homolog 3 (DIAPH3) as the gene responsible fo

90 adation as well as downregulation of protein diaphanous homolog 3 (DIAPH3), a positive regulator of a

91 Diaphanous homologue 1 (DIAPH1) is a Rho effector protei

92 The diaphanous homologue Diaph3 (aka mDia2) is a major regul

93 We identified diaphanous in a screen for genes that are necessary for

94 previous results that identified a role for Diaphanous in apical secretion in tubular organs of Dros

95 f-function approaches to examine the role of Diaphanous in Drosophila morphogenesis.

96 the dia mutant phenotype reveals a role for Diaphanous in recruitment of myosin II, anillin and Pean

97 ession of an activated version of the formin Diaphanous, induced strong overgrowth in Drosophila imag

98 used by RhoB/mammalian homolog of Drosophila diaphanous-induced actin polymerization and RhoA/Rho kin

99 on through interaction between an N-terminal diaphanous inhibitory domain (DID) and a C-terminal diap

100 , through interaction between the N-terminal diaphanous inhibitory domain (DID) and C-terminal diapha

101 ulated by interaction between its N-terminal diaphanous inhibitory domain (DID) and its C-terminal di

102 se binding domain (GBD) that encompasses the diaphanous inhibitory domain (DID) and the carboxyl-term

103 F2 by CAP-KAc-actin is dependent on the INF2 diaphanous inhibitory domain (DID).

104 toinhibitory interaction with the N-terminal diaphanous inhibitory domain (DID).

105 sisting of the GTPase-binding region and the diaphanous inhibitory domain (G-DID), thought to mediate

106 hanism involving intramolecular binding of a Diaphanous inhibitory domain and a Diaphanous autoregula

107 e studies affirm the interaction between the Diaphanous Inhibitory Domain and the cytosolic GTPase do

108 rough an intramolecular interaction with the diaphanous inhibitory domain in the N-terminal half of t

109 These mutations, all within the diaphanous inhibitory domain of INF2, segregate with FSG

110 in spanning residues 129-369 (called DID for diaphanous inhibitory domain) is sufficient for auto-inh

111 The Diaphanous inhibitory domain-dimerization domain (DID-DD

112 disease mutations map to the autoinhibitory diaphanous inhibitory domain.

113 ined six-helix bundle, from which extend two Diaphanous inhibitory domains (DIDs) composed of five ar

114 The second (residues 334-821) covers the Diaphanous inhibitory-dimerization-coiled coil domains,

115 identified a new interaction between Nef and diaphanous interacting protein (DIP), a recently describ

116 ll cytoskeleton, we propose a model in which Diaphanous links receptor tyrosine phosphatase signaling

117 ion studies of wild-type embryos reveal that Diaphanous localizes to the site where the metaphase fur

118 Mammalian Diaphanous (mDia) family formins are Rho-directed effect

119 nd CYK-1, the sole ortholog of the mammalian diaphanous (mDia) family of formins.

120 n Rho and its effector, mammalian homolog of Diaphanous (mDia), in migrating cells, but factors respo

121 ed PANX1 activation involves RhoA, mammalian diaphanous (mDia)-related formin, and a cytosolic lysine

122 Mammalian Diaphanous (mDia)-related formins and the N-WASP-activat

123 Rho GTPase-effector mammalian diaphanous (mDia)-related formins assemble nonbranched a

124 of small-molecule agonists of the mammalian Diaphanous (mDia)-related formins, which act downstream

125 ell characterized, the role of the mammalian Diaphanous (mDia)-related formins, which both nucleate a

126 in parallel, induces a switch from Arp2/3 to Diaphanous-mediated cortical actin nucleation that depen

127 Here, we demonstrate that diaphanous mutations perturb synaptic growth at the NMJ.

128 chanisms regulating formins particularly non-diaphanous ones like FMN1 remain poorly understood.

129 and the formin Diaphanous, and mutations in diaphanous or capping protein beta enhance abl phenotype

130 We have identified mDia1/Drf1 (mammalian Diaphanous or Diaphanous-related formin 1 protein) as a

131 Importantly, loss of Capping proteins and Diaphanous overexpression did not significantly affect c

132 iveness is primarily Enabled driven, whereas Diaphanous plays the primary role in the AS, and reveal

133 xpression of a constitutively active form of diaphanous protein in the auditory organ of Drosophila m

134 The diaphanous protein is a profilin ligand and target of Rh

135 Diaphanous protein is present both presynaptically and p

136 its furrow-extension phenotypes, Peanut and Diaphanous recruitment, and F-actin organization.

137 Knockdown of the actin nucleating protein Diaphanous Related Formin 3 (DRF3/Dia2) by RNA interfere

138 Mammalian diaphanous-related (mDia) formins act as Rho GTPase effe

139 Mammalian Diaphanous-related (mDia) formins are well known for the

140 mall GTP-binding proteins activate mammalian diaphanous-related (mDia) formins by directly binding an

141 ich enhances RhoA-mediated activation of the Diaphanous-related formin (DIAPH1) and targets DIAPH1 to

142 The diaphanous-related formin (DRF) proteins have been ident

143 We have examined the role of the mouse Diaphanous-related formin (DRF) Rho GTPase binding prote

144 r mechanism involves AGER's interaction with diaphanous-related formin 1 (DIAPH1), a formin protein r

145 on of DIAPH1, which encodes the Rho-effector diaphanous-related formin 1 (DIAPH1), as a candidate gen

146 hermore, we show that knockdown of mammalian diaphanous-related formin 1 (mDia1) inhibits chemotactic

147 The murine mammalian Diaphanous-related formin 1 (mDia1) was identified as a

148 and physically interacted with the mammalian diaphanous-related formin 1 (mDia1), a downstream effect

149 We identified mammalian diaphanous-related formin 1 (mDia1), a potent actin nucl

150 entified mDia1/Drf1 (mammalian Diaphanous or Diaphanous-related formin 1 protein) as a PKD2-interacti

151 ssociated activator of morphogenesis-1) is a diaphanous-related formin first studied as a novel dishe

152 Here, we show that the Diaphanous-related formin G (ForG) from the professional

153 Mammalian and fungal Diaphanous-related formin homology (DRF) proteins contai

154 tation in one copy of a tandemly duplicated, diaphanous-related formin is perfectly associated with s

155 We found that diaphanous-related formin mDia2 is a master regulator of

156 Here, we show that loss of diaphanous-related formin mDia2 leads to impaired engraf

157 that the Rif effector in this pathway is the Diaphanous-related formin mDia2.

158 iated activator of morphogenesis (DAAM) is a diaphanous-related formin protein essential for the regu

159 s gene is the founding member of a family of Diaphanous-related formin proteins (DRFs).

160 The diaphanous-related formin, Diaphanous 1 (DIAPH1), is req

161 A mammalian Diaphanous-related formin, mDia1, localizes at the jagge

162 DIAPH1 encodes the mammalian Diaphanous-related formin, mDia1.

163 Elongation proteins, Diaphanous-related formin, VASP, and fascin are recruite

164 Mammalian Diaphanous-related formins (Drfs) act as Rho small GTPas

165 Diaphanous-related formins (DRFs) are key regulators of

166 stelium model system, we show that the three Diaphanous-related formins (DRFs) ForA, ForE, and ForH a

167 However, the activation mechanism for these Diaphanous-related formins (DRFs) is not completely unde

168 Diaphanous-related formins (DRFs) regulate dynamics of u

169 INF2 interacts with diaphanous-related formins (mDia) and antagonizes mDia-m

170 In addition, the Diaphanous-related formins 1-3 (mDia1-3) localized to pr

171 In cultured cells, Diaphanous-related formins also regulate cell adhesion,

172 actin-filament assembly that is mediated by Diaphanous-related formins and activators of Arp2/3, res

173 The Diaphanous-related formins are effectors for Rho GTPases

174 Diaphanous-related formins are eukaryotic actin nucleati

175 ndocytosis is controlled by actin regulatory diaphanous-related formins mDia1/3 and Rho family GTPase

176 Unlike diaphanous-related formins, FMN1 is not subject to canon

177 One family of actin filament nucleators, the Diaphanous-related formins, is activated by the binding

178 Diaphanous-related formins, the best-known subclass, are

179 The DIAPH (Diaphanous-related) family of formins promote both actin

180 uses several downstream effectors, including Diaphanous, Rok, and Pkn, simultaneously to mediate its

181 However, redundancy in mammals and Diaphanous' role in cytokinesis limited analysis of whet

182 la melanogaster embryos, we demonstrate that Diaphanous, SCAR, and WASp play distinct but overlapping

183 The basal cytonemes required diaphanous, SCAR, Neuroglian and Synaptobrevin, and both

184 al functions of FH proteins, we propose that Diaphanous serves as a mediator between signaling molecu

185 Genetic loss-of-function conditions for diaphanous, shibire, neuroglian, and capricious perturbe

186 We used constitutively active Diaphanous to examine its roles in morphogenesis and its

187 Male-sterile alleles of chickadee and diaphanous, which are deficient in germ cells, exhibit s

188 We find Rho kinase and Diaphanous, which facilitate mitotic cell rounding in co

189 Rho pathway, such as pebble, racGAP50C, and diaphanous, which had profound effects on furrowing but