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1 mine whether CTRP5 and MFRP are functionally dicistronic.
2 gene, and these two genes are reported to be dicistronic.
3 CAT-LUC RNA, a helper-dependent cDNA-encoded dicistronic analog of RSV genomic RNA in which the viral
4 B10 and pMHB11 constructs described here are dicistronic and encode genes for herbicide (bar) and spe
5                                 The locus is dicistronic and encodes two distinct proteins, stoned A
6 istronic and encodes Adh alone, the other is dicistronic and includes the open reading frames of both
7 more stringent studies, such as promoterless dicistronic and monocistronic DNA and dicistronic RNA te
8 ds, such as dicistronic RNA and promoterless dicistronic and monocistronic DNA assays.
9                          This undermines the dicistronic assay, wherein IRES activity is measured by
10                                     Although dicistronic assays are the canonical method of testing s
11                                 In addition, dicistronic assays establish that the 5'-UTR region func
12                                          The dicistronic atpA-orf522 transcripts are preferentially d
13 lted in readthrough transcription to yield a dicistronic CAT-LUC mRNA.
14 pression of the downstream RDRP:GFP from the dicistronic construct CP-RDRP:GFP could not be detected.
15 at low levels from its downstream ORF in the dicistronic construct in S. pombe cells.
16 nticipated monocistronic RNAs arise from the dicistronic construct used.
17                               We have made a dicistronic construct where the picornaviral internal ri
18 essed from the first cistron from all of the dicistronic constructs introduced into the fly genome.
19 eader sequence or upstream of either CIRE in dicistronic constructs markedly increased their regulato
20  second cistron was not expressed in control dicistronic constructs that lacked these sequences or co
21 ne sh-ble as a reporter in monocistronic and dicistronic constructs, and consistently yielded higher
22 suggested from studies using the traditional dicistronic DNA assay that the expression of p27kip1 is
23  Considering the inevitable drawbacks of the dicistronic DNA assay, which could mislead a promoter ac
24  first and second coding sequences to form a dicistronic DNA fragment.
25 re, we confirm that ORF35-37 is functionally dicistronic during infection and demonstrate that mutati
26 1 RNA is exceptional because it is naturally dicistronic, encoding two proteins essential for retrotr
27 ample of a naturally occurring, functionally dicistronic eukaryotic mRNA whose translation is control
28 eam cistron encoding Firefly luciferase in a dicistronic expression vector.
29 ogous RNA must be considered when IRES-based dicistronic expression vectors are being constructed.
30                  The anaerobically inducible dicistronic focA-pfl operon is transcribed from three co
31                          For Ad vectors, the dicistronic fragment was inserted between a cytomegalovi
32                           In Rv vectors, the dicistronic fragment was inserted between the 5' long te
33  and G-418 resistance to be transcribed as a dicistronic fusion mRNA under the control of the phospho
34 tween the CAT and lacZ coding sequences in a dicistronic gene and tested for IRES activity in transge
35 ressing the CFTR gene, the expression of the dicistronic gene can be detected by lacZ staining and fo
36 n of two independent protein products from a dicistronic gene with almost complete efficiency.
37 stem for dependable high-level expression of dicistronic genes in Chlamydomonas reinhardtii has been
38 on for instability of the genome is that the dicistronic genome was at least 108% larger than the wil
39                                          One dicistronic genome was constructed in which the poliovir
40 is unique amongst picornaviruses in having a dicistronic genome with internal ribosomal entry sites (
41                                     A second dicistronic genome, EMCV-Luc-PV, was constructed with th
42    We designed a series of monocistronic and dicistronic GluR2 cDNA constructs that model the natural
43 h the nonstructural proteins, we constructed dicistronic HAV genomes in which a heterologous picornav
44 on-competent, revertant viruses rescued from dicistronic HRV-14 RNAs containing the poliovirus cre, o
45 rotein knockdown by RNA interference using a dicistronic lentivirus small hairpin (shRNA) delivery sy
46 mon exon into the intercistronic region of a dicistronic luciferase construct, yielded luciferase act
47                                  Utilizing a dicistronic luciferase mRNA reporter containing the GATA
48  RNA replication defect in dicistronic PV or dicistronic luciferase replicons in vivo.
49                       Here, we show by using dicistronic luciferase reporter vectors that the 5'-UTR
50 th 16 CGG repeats for both monocistronic and dicistronic (luciferase) reporter constructs.
51 ion unit and are most likely produced from a dicistronic message.
52                                            A dicistronic minigenome containing the M-F gene junction
53 on of full-length mRNA and, in the case of a dicistronic minigenome, sequential transcription.
54       We have previously shown, using an SV5 dicistronic minigenome, that replacement of the 22-base
55 red by their individual incorporation into a dicistronic minigenome.
56                             In two different dicistronic minigenomes, transcription of each gene was
57 alyzed in helper-dependent monocistronic and dicistronic minireplicons which were complemented by a s
58 edback loop that escalates the production of dicistronic mRNA and Gal4/VP16.
59 rotransposition, L1 RNA functions first as a dicistronic mRNA and then as a template for cDNA synthes
60 we found no evidence of IRES function when a dicistronic mRNA containing the eIF4G sequence was trans
61                                       When a dicistronic mRNA containing the pim-1 5'-UTR was transla
62 upstream of both ORFs (ORF1 and ORF2) in the dicistronic mRNA encoded by mouse L1.
63 n initiated from the 5' LTR would generate a dicistronic mRNA for the HBsAg and B7-1 molecules.
64 s the translation of the second cistron in a dicistronic mRNA in cultured mammalian cells.
65 ite, expression from the distal cistron of a dicistronic mRNA increased as a function of the length o
66 resent evidence that the accumulation of the dicistronic mRNA is controlled at the level of the 3' en
67                                  Because the dicistronic mRNA is not processed, we could show that pr
68                           The genome-length, dicistronic mRNA of the double-stranded RNA fungal virus
69  resulting in inhibition of IRES function in dicistronic mRNA reporter assays.
70 RES module in the intercistronic region of a dicistronic mRNA strongly enhances IRES activity in vari
71 om a transcript shorter than the full-length dicistronic mRNA was found.
72     This method uses a vector that encodes a dicistronic mRNA with a reporter gene (Renilla luciferas
73 lishes the formation of the major nad4L-atp4 dicistronic mRNA with the -318 5' terminus, the presence
74 en present in the intercistronic region of a dicistronic mRNA, indicating that the leader contains an
75                                         In a dicistronic mRNA, the presence of the TEV 5' leader sequ
76 icistronic vector produces only the intended dicistronic mRNA.
77 nce the translation of a second cistron in a dicistronic mRNA.
78 sayed for IRES activity in a dual luciferase dicistronic mRNA.
79        In addition, we provide evidence that dicistronic mRNA:tRNA transcripts are frequently produce
80 ure that uses a retroviral vector to express dicistronic mRNAs encoding enhanced green and cyan fluor
81 ient translation of a second URA3 cistron in dicistronic mRNAs in S. cerevisiae, thereby conferring u
82 RES enhanced translation of monocistronic or dicistronic mRNAs in vitro under competitive conditions,
83 and in vivo, as can be shown with artificial dicistronic mRNAs or with chimeric viral genomes in whic
84 e splicing of a single gene, and we identify dicistronic mRNAs that each encode two receptors.
85 d translation from uncapped monocistronic or dicistronic mRNAs to a greater extent than did eIFiso4F.
86  studies, a library of constructs expressing dicistronic mRNAs with the HIS3 gene as the second cistr
87  when tested in the intercistronic region of dicistronic mRNAs, both sequences were shown to contain
88 CV IRES function in the context of mono- and dicistronic mRNAs.
89 on in foreign viral genomes or in artificial dicistronic mRNAs.
90 n permits quantification of both products of dicistronic mRNAs.
91 ed the presence of the predicted full-length dicistronic mRNAs.
92  sequences into the intercistronic region of dicistronic mRNAs.
93 CV IRES function in the context of mono- and dicistronic mRNAs.
94 eration of polymorphic 5' extremities of the dicistronic nad3-rps12 and nad4L-atp4 transcripts to the
95 eat proteins, are required for processing of dicistronic nad4L-atp4 and nad3-rps12 transcripts in Ara
96                            A LytSR-regulated dicistronic operon has also been identified and shown to
97  Here, we show that crhR is expressed from a dicistronic operon with the methylthiotransferase rimO/m
98                           It is located in a dicistronic operon with the yjbC gene.
99 ream of narK, the first gene in the narK-fnr dicistronic operon.
100 was present downstream of other sequences in dicistronic or chimeric transcripts, the RNAs were no lo
101                                            A dicistronic plasmid expressing both gp120 and IL-2 induc
102                                We designed a dicistronic plastid marker system that relies on the pla
103                               Although these dicistronic poliovirus genomes were replication competen
104 ddress this possibility, we have constructed dicistronic poliovirus replicons by substituting the EMC
105 on have been dispelled by constructing novel dicistronic poliovirus replicons with the gene order [PV
106                                      Using a dicistronic poliovirus RNA, we were able to functionally
107                       Here, we show that the dicistronic precursor containing tRNAvalV and tRNAvalW u
108                                          The dicistronic precursor tRNA is present both in the cytoso
109                                              Dicistronic precursors containing the tRNA(1)(Ser) and t
110  to complement the RNA replication defect in dicistronic PV or dicistronic luciferase replicons in vi
111 olic labeling of monocistronic mRNAs and the dicistronic readthrough RNAs that result when terminatio
112 serial passages with VV-P1 revealed that the dicistronic replicon was encapsidated more efficiently t
113 e of the poliovirus P1 region; the resulting dicistronic replicons are smaller than the wild-type pol
114                                         Both dicistronic replicons expressed abundant luciferase foll
115              To analyze encapsidation of the dicistronic replicons, we used a system in which the cap
116 be promoters, mimic both types of element in dicistronic reporter assays by causing these cryptic rea
117 translation of the GADD45 alpha protein in a dicistronic reporter gene activity assay.
118 lization of monocistronic gfp transcripts in dicistronic reporter lines, adding to the evidence that
119                                      Using a dicistronic reporter system and a green fluorescent prot
120                                        Using dicistronic reporter systems for IRES detection, we dete
121    We compared the translational activity of dicistronic reporter transcripts containing the HCV IRES
122                                    We used a dicistronic reporter vector to test whether brain-derive
123                                       When a dicistronic reporter vector, with c-myc 5' UTR inserted
124                                        Using dicistronic reporters carrying mutations in the initiati
125               T cells were transduced with a dicistronic retroviral vector, NIT, which encodes low-af
126 omoter using more stringent methods, such as dicistronic RNA and promoterless dicistronic and monocis
127 us Cricket Paralysis virus contains a unique dicistronic RNA genome arrangement, encoding two main op
128 trix (M) gene end (3'-UCAAUUAUUUUUU-5') in a dicistronic RNA replicon.
129 erless dicistronic and monocistronic DNA and dicistronic RNA tests, are required to safeguard any fut
130    We analyzed the translational activity of dicistronic RNA transcripts containing wild-type or muta
131                                           In dicistronic RNA, the RTS enhances cap-dependent translat
132             We report here that packaging of dicistronic RNA3 requires a bipartite signal.
133 s hypothesis, we examined the translation of dicistronic RNAs containing upstream and downstream repo
134                       Additional selectable, dicistronic RNAs encoding NS2-NS5B, E1-NS5B, or the full
135 ection of permissive FRhK-4 cells with these dicistronic RNAs failed to result in the rescue of infec
136      However, when capped and polyadenylated dicistronic RNAs were synthesized in vitro and transfect
137                                          The dicistronic RSV-CAT-LUC minigenome is a derivative of RS
138                                              Dicistronic, selectable subgenomic replicons derived fro
139                                         In a dicistronic SH-HN minigenome, a U4-G combination was fun
140                               The Drosophila dicistronic stoned locus encodes two distinctive presyna
141 osition 1049 (L(1049N) or L(1049D)) by using dicistronic subgenomic replicons and found that the alte
142 ed the RNA synthetic activities of mono- and dicistronic subgenomic replicons of RS virus by direct m
143                                         Nine dicistronic subgenomic replicons of RS virus were constr
144 the feasibility and utility of 2A-containing dicistronic systems that rely on a strong conditional pr
145                            Both promoterless dicistronic test and northern blot analysis show transcr
146 (i.e., mite)-related parallel evolution from dicistronic to monocistronic genomic form within the fam
147 he D. pseudoobscura cluster also generates a dicistronic transcript from a gene pair analogous to the
148                                          The dicistronic transcript is found in polysomes and the Adh
149                Accumulation of the ycf9-psbM dicistronic transcript was disrupted in the revertants,
150 entral gene (gene2) is transcribed only as a dicistronic transcript with Acam.
151 and Adh-related (Adhr), are transcribed as a dicistronic transcript.
152 nd the apportionment of monocistronic versus dicistronic transcription of both genes appears to be su
153 thesis, we constructed plasmids containing a dicistronic transcriptional unit in which the HAV IRES w
154 e transfected cells with plasmids expressing dicistronic transcripts in which the upstream cistron en
155 osite to that of the prediction and possible dicistronic transcripts.
156                                          The dicistronic transgenic strains bearing the Ubx and Antp
157 uch as A-to-I editing, alternative splicing, dicistronic translation, and selenocysteine incorporatio
158 nce of these events suggests that the 7.5 kb dicistronic unit is suitable for constructing Leishmania
159 V40 T antigen and H-ras val12 oncogenes as a dicistronic unit separated by a poliovirus internal ribo
160 e can be expressed in mammalian cells from a dicistronic vector and has been purified using sequentia
161                             The promoterless dicistronic vector might therefore prove useful in futur
162 n checked carefully to determine whether the dicistronic vector produces only the intended dicistroni
163                                By applying a dicistronic vector strategy on transfected cell lines, w
164 -myc, and demonstrated by transfections in a dicistronic vector system that it contains a potent inte
165  this concern, we created a new promoterless dicistronic vector to test the putative IRES derived fro
166  testing is carried out using a conventional dicistronic vector, Northern blot analyses might not be
167                       Using the promoterless dicistronic vector, we also found promoter activities in
168                                              Dicistronic vectors in which ORF1 and ORF2 were substitu
169 , devised to rule out splicing in tests with dicistronic vectors, are not valid and why experiments w
170                     In this study, we used a dicistronic vesicular stomatitis virus (VSV) minigenome
171                                 We have used dicistronic vesicular stomatitis virus (VSV) minigenomes
172            By introducing the transcripts of dicistronic viral constructs into Giardia, we demonstrat
173 f viruses that are evolutionarily related to dicistronic viruses but contain a single open reading fr
174 R analysis confirmed strong induction at the dicistronic Wbeta locus and at four other phage loci in
175  evidence of recombination was observed at a dicistronic Wbeta locus, encoding putative bacterial cel

 
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