ライフサイエンスコーパス: directed evolution

1 ationally designed hydrophobic pocket during directed evolution.

2 he opportunity to improve ArM performance by directed evolution.

3 ubstrate was enhanced more than 1000-fold by directed evolution.

4 e and they have yet to be engineered through directed evolution.

5 g site of an enzyme that can be subjected to directed evolution.

6 or those at others) is a notable feature of directed evolution.

7 ecular-recognition motifs identified through directed evolution.

8 retroaldolase designs prior to comprehensive directed evolution.

9 seek improved catalytic characteristics via directed evolution.

10 made by chimeragenesis, rational design, or directed evolution.

11 engineered RsRubisco, an isoform amenable to directed evolution.

12 previously realized from rational design or directed evolution.

13 starting points for further optimization by directed evolution.

14 protein properties using techniques such as directed evolution.

15 new approach to optimizing gene networks via directed evolution.

16 tions are needed to stabilize proteins using directed evolution.

17 riants that may emerge in natural as well as directed evolution.

18 synthetase mutants with new specificities by directed evolution.

19 ility and recombinant expression yield using directed evolution.

20 previously optimized for expression through directed evolution.

21 euticals or biosensors, and as templates for directed evolution.

22 ich is typically improved upon by laboratory-directed evolution.

23 otein cages through rational engineering and directed evolution.

24 and that these activities can be improved by directed evolution.

25 te promoting vibration was introduced during directed evolution.

26 vided additional mutations are introduced by directed evolution.

27 ll as for materials design, fabrication, and directed evolution.

28 sually carried out by rational design and/or directed evolution.

29 ning focused protein libraries to be used in directed evolution.

30 anced the thermal stability of Cel7A through directed evolution.

31 is a common task in protein engineering and directed evolution.

32 d mutagenesis, computational refinement, and directed evolution, a modestly active, computationally d

33 Using directed evolution, a variant N-acetyl amino acid racema

34 shes a generic platform for rapid eukaryotic-directed evolution across an array of target cargo.

35 Optimization of this activity by directed evolution afforded an efficient enzyme variant

36 DNA approaches have enabled the extension of directed evolution algorithms to the genome-scale.

37 In contrast, directed evolution also yielded CBL(P113S), which cataly

38 we combine ancestral protein reconstruction, directed evolution and biophysical analysis to explore a

39 Here we use a combination of directed evolution and chemical and biochemical approach

40 fluorescent properties of iLOV, we employed directed evolution and determined five LOV crystallograp

41 ssayed, has emerged as a powerful format for directed evolution and functional metagenomics but is cu

42 these new technologies within the context of directed evolution and inverse metabolic engineering.

43 ning assays represents a major bottleneck in directed evolution and limits the improvements that are

44 Directed evolution and protein engineering approaches us

45 Extensive directed evolution and protein engineering resulted in s

46 ting Evolutionary Adaptive Paths (REAP), for directed evolution and protein engineering that exploits

47 ering new structural folds experimentally by directed evolution and random recombination of secondary

48 hermal green protein (TGP) and eCGP123 using directed evolution and ratiometric sorting.

49 In addition, modern methods of directed evolution and synthetic biology, especially tho

50 powerful techniques of genetic engineering, directed evolution, and biomimetics.

51 tural information, bacterial selection-based directed evolution, and combinatorial design.

52 n fields as varied as genomics, diagnostics, directed evolution, and drug screening.

53 onomeric FP, for enhancing its brightness by directed evolution, and for thoroughly characterizing th

54 A directed evolution approach has been used for the genera

55 oteins for therapeutic applications, and our directed evolution approach may also enable exploration

56 Taken together, the directed evolution approach reveals an unexpectedly flex

57 We used a directed evolution approach to examine the molecular mec

58 ormous potential of a combined computational/directed evolution approach to protein engineering.

59 Here, using a directed evolution approach with our two previously desc

60 First, with a directed evolution approach, we isolated mutations in D1

61 re then further optimized using a FACS-based directed evolution approach.

62 port in brain, was generated in mice using a directed evolution approach.

63 either partner, enabling the application of directed evolution approaches for high-throughput charac

64 Inspired by the great success of directed evolution approaches in tailoring enzyme proper

65 Herein, we have combined rational design and directed evolution approaches to test this hypothesis.

66 In contrast to other in vivo directed evolution approaches, CPR largely mitigates hos

67 ith higher fitness than those found by other directed evolution approaches.

68 Biocatalyst discovery and directed evolution are central to many pharmaceutical re

69 d mutants of this monooxygenase generated by directed evolution are excellent catalysts for the oxida

70 absolute catalytic effect and the effect of directed evolution are reproduced and analyzed (assuming

71 enzymes created by computational design and directed evolution are versatile biocatalysts whose prom

72 Directed evolution, artificial selection toward designed

73 ombinatorial strategy of rational design and directed evolution as a powerful tool for the discovery

74 enantioselectivity proved to be possible by directed evolution based on saturation mutagenesis (up t

75 ch design-based strategies, we report here a directed evolution-based method for generating optimal b

76 With the development of directed evolution beginning in the 1990s and continuing

77 This genetic strategy of directed evolution bestows a next-generation of AAVP par

78 erase orthologues from the genus Thermus and directed evolution by CSR in the presence of inhibitors.

79 the experimental library-size limitations of directed evolution by focusing diversity toward the posi

80 Such methods accelerate directed evolution by learning from the properties of ch

81 h smaller, nonantibody therapeutics, we used directed evolution by yeast-surface display to engineer

82 Therefore a directed evolution campaign with two rounds of random mu

83 libraries reduce protein screening effort in directed evolution campaigns by focusing on a limited nu

84 ld take approximately 3-5 d, whereas a whole directed evolution can be performed in 3-10 rounds, depe

85 Our results show how directed evolution circumvents challenges inherent to en

86 massive libraries of plasmids as vectors for directed evolution, combinatorial gene circuit tests, an

87 enzymes created by computational design and directed evolution constitute a potentially valuable sou

88 ET94 (PpDyP) and three variants generated by directed evolution (DE) are studied aiming at the design

89 with the more empirical methods of classical directed evolution (DE) for improving kcat (where natura

90 We note that directed evolution differs in a number of ways from natu

91 e activity of TEM, have been coupled using a directed evolution domain insertion approach.

92 engineering through machine-learning-guided directed evolution enables the optimization of protein f

93 ms in immunology we may be able to perform a directed evolution experiment where we follow the evolut

94 ortantly, the consensus binder isolated from directed evolution experiments (6.2.18) forms a higher o

95 Directed evolution experiments identified I-TevI variant

96 Here, using directed evolution experiments with the GIY-YIG homing e

97 te state machines-which can be inferred from directed evolution experiments.

98 , demonstrating that machine learning-guided directed evolution finds variants with higher fitness th

99 in bacteria, where they can be optimized by directed evolution for a broad spectrum of enantioselect

100 uced in bacteria, where they can be tuned by directed evolution for activity and selectivity.

101 this variant was then further engineered via directed evolution for practical application in a manufa

102 PTE has previously proven amenable to directed evolution for the improvement of catalytic acti

103 tal test of evolutionary reversibility using directed evolution from a phosphotriesterase to an aryle

104 Directed evolution generated 70 GR variants with absorpt

105 We highlight recent cases in which directed evolution generated enzymatic activities and su

106 Additional directed evolution generated two enzymes having NADH-dep

107 entary proton transfer reaction, we show how directed evolution gradually altered the conformational

108 is now supports our original hypothesis that directed-evolution had generated an evolutionary interme

109 Engineering and study of protein function by directed evolution has been limited by the technical req

110 In the laboratory, directed evolution has emerged as a powerful tool for th

111 Directed evolution has proved to be an effective strateg

112 Over the past two decades, directed evolution has transformed the field of protein

113 IFRS, a product of directed evolution, has distinct binding modes for diffe

114 mputational design approaches, combined with directed evolution, has provided an opportunity to revis

115 Advances in directed evolution have led to an exploration of new and

116 Directed evolution identified two point mutations that i

117 emonstrate its power, we apply the system to directed evolution, identifying new mutants of the enzym

118 of in silico molecular docking and in vitro directed evolution in a well-characterized drug-sensitiv

119 ntinuous evolution (PACE) enables continuous directed evolution in bacteria by mapping the steps of D

120 e the underlying genetic mechanisms, we used directed evolution in Escherichia coli to accumulate var

121 We previously used directed evolution in human airway epithelia to create a

122 Here, we present a system for facile directed evolution in mammalian cells.

123 We have initiated a program of directed evolution in order to create mutant amide synth

124 onstrated the application of CRISPR-Cas9 for directed evolution in rice, engineering crops for desire

125 ts to RNA aptamers using rational design and directed evolution in Saccharomyces cerevisiae.

126 of wild-type AAV genomes from tissues during directed evolution in the absence of wild-type adenoviru

127 By directed evolution in the laboratory, we previously gene

128 the gene encoding pocr to several rounds of directed evolution in which codons for the corresponding

129 Directed evolution, in addition to its principal applica

130 ncrease affinity are typically identified by directed evolution involving combinatorial libraries.

131 Directed evolution is a powerful strategy for protein en

132 Directed evolution is a powerful tool to improve the cha

133 Directed evolution is a powerful, but experimentally str

134 Directed evolution is a valuable technique to improve en

135 Directed evolution is applied to increase the dynamic ra

136 and their improved variants from laboratory directed evolution (LDE), using atomistic simulations wi

137 Optimization by directed evolution led to the identification of two prom

138 In an analysis of a directed evolution longitudinal yeast data set, we are a

139 ifying bacteria using genetic engineering or directed evolution, mass culturing and controlling the a

140 nered replication (CPR) is an emulsion-based directed evolution method based on a robust and modular

141 ELMA (SELection with Modified Aptamers) is a directed evolution method which can be used to develop D

142 Using a directed-evolution method that takes advantage of the na

143 In turn, directed evolution methodologies, which consist of itera

144 While directed evolution methods have provided valuable tools

145 Most existing directed evolution methods, both in vivo and in vitro, s

146 When combined with directed evolution, molecular dynamics simulations, and

147 Unexpectedly, it is found that the directed evolution mutants lead to the reduction of solv

148 Here we report the directed evolution of a ligand trap for Ang2 by harnessi

149 We use this system to perform the directed evolution of a phosphotriesterase (a bioremedia

150 Directed evolution of a serine-ligated P450 variant, P41

151 Here we describe a strategy using in vitro-directed evolution of a single TCR to change its peptide

152 of its negatively charged amino acids using directed evolution of a synthetic form of Ocr, termed po

153 recombinant AAV-packaging cell lines and the directed evolution of AAV capsids.IMPORTANCE We first re

154 ficial phylogeny emerging in three rounds of directed evolution of an amine dehydrogenase biocatalyst

155 Mix Library on Beads-was applied towards the directed evolution of an anti-IgE Affibody (ZIgE), gener

156 Here we report the directed evolution of an iron-containing enzymatic catal

157 Directed evolution of Arabidopsis thaliana phytochelatin

158 paving the way toward the rational design or directed evolution of artificial ComRS systems for a ran

159 This protocol describes the directed evolution of artificial endonuclease and ligase

160 Directed evolution of B. napus DGAT1 (BnaDGAT1) previous

161 s activated alpha(IotaIotab)beta(3), we used directed evolution of beta(3)(K716A) to identify substit

162 The directed evolution of biomolecules to improve or change

163 sign a new M13 phagemid-based system for the directed evolution of biomolecules.

164 Thus, the knowledge generated from directed evolution of DGAT1 in one plant species can be

165 zed bead labelling and demonstrate it by the directed evolution of efficient RTs for 2'-O-methyl RNA

166 hus candidate parts for rational redesign or directed evolution of efficient, nonsuicidal THI4s for u

167 To investigate this phenomenon, we used directed evolution of EmrE to assess the rate of develop

168 many new applications: for example, assay of directed evolution of enzymes catalyzing halo-metabolite

169 The directed evolution of enzymes is now routinely used to d

170 protein engineering permit the design and/or directed evolution of enzymes specifically tailored for

171 powerful platform for single cell analysis, directed evolution of enzymes, and high throughput scree

172 , which exhibits features reminiscent of the directed evolution of enzymes, delivers catalysts that c

173 Here, we performed comparative directed evolution of four orthologous metallo-beta-lact

174 Here, we employed yeast surface display for directed evolution of full-length human TIMP-1 to develo

175 echnology enables the in vitro selection and directed evolution of functional proteins from libraries

176 escribe a system that enables the continuous directed evolution of gene-encoded molecules that can be

177 Directed evolution of genetically encoded cytochrome P41

178 The reporter enabled in vivo directed evolution of Gerbera hybrida 2-pyrone synthase

179 Directed evolution of maize HPPD was accomplished by pro

180 ways, by changing its population size and by directed evolution of new functions.

181 Directed evolution of PLD using yeast membrane display a

182 ped a generalizable yeast-based platform for directed evolution of protease catalytic properties.

183 Here we describe a system for the continuous directed evolution of proteases using phage-assisted con

184 munized animals and/or performing laboratory-directed evolution of proteins with an existing low affi

185 in libraries, and should enable the in vitro directed evolution of proteins with designer single-mole

186 ggesting that PERMUTE will be useful for the directed evolution of proteins with new functions.

187 Using directed evolution of RlmN under antibiotic selection, w

188 Directed evolution of Rma cyt c in the bacterial catalys

189 e exonucleolytic proofreading as well as the directed evolution of RNA RTs with very high complementa

190 Here we describe a general strategy for the directed evolution of RT function for any template chemi

191 Through directed evolution of SecB, we have identified and chara

192 Early studies on the directed evolution of serine recombinase DNA sequence sp

193 hesis of P-methyl and P-ethyl phNAs, and the directed evolution of specific streptavidin-binding phNA

194 a selection mechanism for the isolation and directed evolution of ternary complexes where unnatural

195 However, directed evolution of terpene synthases is hampered by t

196 Here, we show that structure-guided directed evolution of the active site of the cytochrome

197 nstrate our approach using as an example the directed evolution of the bacteriophage lambda cI TF aga

198 Directed evolution of the first Kunitz domain of TFPI1 h

199 Herein, we report the directed evolution of the fluorinase FlA1 for improved c

200 We conducted directed evolution of the Ir(Me)-myoglobin and generated

201 Via directed evolution of the natural iron-sequestering ferr

202 Directed evolution of the ribosome for expanded substrat

203 most active construct, W(3Cyto), served for directed evolution of the three cytoplasmic Trps, where

204 creen and the first proof of concept for the directed evolution of this enzyme class toward the ident

205 Overall, the directed evolution of three different designed Kemp elim

206 amatically decreases the screening effort in directed evolution of transaminases, as only active vari

207 Directed evolution of two computationally designed Kemp

208 gainst an entirely combinatorial approach of directed evolution of vast protein libraries.

209 Synthetic biology, genome engineering and directed evolution offer innumerable tools to expedite e

210 Directed evolution offers an attractive alternative prot

211 y of recombinases can be altered either with directed evolution or via fusions to modular DNA-binding

212 However, many desirable targets of directed evolution perform poorly or unnaturally in unic

213 Using a continuous directed evolution platform called OrthoRep, we rapidly

214 nology, both our evolved TEV mutants and the directed-evolution platform used to generate them could

215 y provide solutions to otherwise intractable directed evolution problems and address novel questions

216 ere included in the libraries throughout the directed evolution process.

217 Directed evolution produced an unexpected combination of

218 bility to optimize non-natural reactivity by directed evolution promises to yield exceptional catalys

219 Enzyme performance was optimized using directed evolution protocols adapted to an expanded gene

220 uce a high-fidelity, high-throughput Rubisco-directed evolution (RDE2) screen that negates false-posi

221 screening of 17 cut sites in HRP followed by directed evolution, reconstitute into an active form whe

222 Directed evolution relies on both random and site-direct

223 Directed evolution relies on iterative cycles of randomi

224 Directed evolution remains a powerful, highly generaliza

225 Six generations of directed evolution resulted in TrpB Pf(quat) with a 400-

226 eliminated by using protein engineering and directed evolution, resulting in superior probes for use

227 We utilized a combination of directed evolution, saturation mutagenesis, chemical mod

228 Using this assay as a directed evolution screen, we demonstrate the generation

229 We then applied directed evolution so as to alter the recognition of a p

230 ully genetically encoded and configurable by directed evolution so that just a few mutations to the e

231 Here, we describe a directed evolution strategy for enzymes that catalyze, i

232 s this problem, we have created a two-tiered directed evolution strategy in Escherichia coli that ena

233 We propose that this structure-directed evolution strategy might be generally applicabl

234 Directed evolution studies are enabled through the const

235 nction, in no insignificant part a result of directed evolution studies, are increasingly empowering

236 spect that is often overlooked in laboratory directed evolution studies.

237 and also provides support for mutagenesis or directed evolution studies.

238 indicating that this method can be used for directed evolution studies.

239 Here we applied directed evolution techniques to create magnetic resonan

240 1) as a proof of principle, we show that two directed evolution technologies that we recently develop

241 ctivity and give insight into the process of directed evolution that gave rise to this family of neut

242 Here we describe a versatile platform for directed evolution that rapidly selects for reverse tran

243 Using directed evolution, the properties of a beta-class CA fr

244 everal useful RFPs have been developed using directed evolution, the quest for further red-shifted an

245 GlcNAc(2)GlcN(2), sufficed to identify, in a directed evolution, the site-saturation mutagenesis libr

246 Non-natural activities can be improved by directed evolution, thus mimicking the process used by n

247 Five enzymes were engineered through directed evolution to act on non-natural substrates.

248 Here we apply part mining and directed evolution to build a set of transcriptional AND

249 e engineering of a cytochrome P450 enzyme by directed evolution to catalyze metal-oxo-mediated anti-M

250 e combine substrate specificity analysis and directed evolution to develop a diverse collection of Gi

251 This constitutes the first reported use of directed evolution to enable the functionalization of su

252 To address this need, we applied in vivo directed evolution to engineer potent retrograde functio

253 Here we employ directed evolution to engineer TrpB to accept 3-substitu

254 alization of cyclic targets and the power of directed evolution to enhance the scope of new-to-nature

255 or production of Trp analogs and for further directed evolution to expand substrate and reaction scop

256 We used directed evolution to explore allosteric regulation as a

257 We used directed evolution to identify mutations that dramatical

258 xhibit low catalytic efficiencies, requiring directed evolution to improve activity.

259 that are being developed to allow us to use directed evolution to improve enzyme properties, often d

260 ll molecules is dependent upon techniques in directed evolution to improve production titers.

261 To this end, we used in vitro-directed evolution to increase the affinity of human CD4

262 isplay of Mac-1 inserted (I) domain library, directed evolution to isolate active mutants of the I do

263 onto the bacteriophage life cycle and allows directed evolution to occur on much faster timescales co

264 inuous evolution, which enables the steps of directed evolution to proceed seamlessly over many succe

265 cally disordered region has been modified by directed evolution to quantitatively assess the thermody

266 Here, we combine rational design and directed evolution to re-engineer the serine recombinase

267 We have applied directed evolution to TrpB from Pyrococcus furiosus and

268 tilizing structure-based rational design and directed evolution, to enhance the peptide binding affin

269 E(p4), a variant previously obtained through directed evolution, transforms several chlorobiphenyls,

270 Optimized using directed evolution, TurboID has substantially higher act

271 populations of yellow fluorescent protein to directed evolution under different selection regimes and

272 motifs from mutant libraries by experimental directed evolution under no co-evolutionary constraints.

273 sue factor pathway inhibitor 1 (TFPI1 D2) to directed evolution using phage display to yield inhibito

274 rom the 14-kDa photoactive yellow protein by directed evolution using yeast display and fluorescence-

275 gineering that combines rational design with directed evolution, using a yeast surface display high-t

276 Directed evolution, using active-site randomization and

277 Structure-based directed evolution utilizing iterative saturation mutage

278 thousands of P450 variants and comprehensive directed evolution via random and targeted mutagenesis.

279 Directed evolution was applied to enhance this non-nativ

280 op reagents suitable for diagnosis, in vitro directed evolution was applied to identify consensus pep

281 cle, the only previous example of continuous directed evolution was the landmark study of Wright and

282 Directed evolution was used to discover a further gain-o

283 Directed evolution was used to engineer a small, disulfi

284 Taking advantage of directed evolution, we analyzed the stepwise oxygen-inse

285 Using directed evolution, we engineered a 'decoy-resistant' IL

286 Using directed evolution, we engineered a high-affinity cameli

287 Using directed evolution, we engineered cytochrome P450 enzyme

288 Using directed evolution, we enhanced the catalytic function o

289 Using directed evolution, we have engineered a hemoprotein bio

290 Using directed evolution, we isolated mutants of the beta and

291 odule for protein engineering, two rounds of directed evolution were performed to improve the activit

292 mining the structures of enzymes produced by directed evolution, where the specificity determinants m

293 Directed evolution, which applies the principles of Darw

294 e upon the catalytic rate, thereby providing directed evolution with better starting sequences for in

295 By combining directed evolution with deep sequencing, it is now possi

296 plex automated genome engineering (MAGE) and directed evolution with random genomic mutations (DIvERG

297 f five artificial retro-aldolase enzymes via directed evolution, with the final variant exhibiting a

298 ts on throughput imposed during the standard directed evolution workflow (library construction, trans

299 ly, we incorporate machine learning into the directed evolution workflow.

300 With SpliMLiB, directed evolution workflows are accelerated by integrat