ライフサイエンスコーパス: directed mutagenesis

1 The two activities can be separated by site-directed mutagenesis.

2 etically engineer these regions through site-directed mutagenesis.

3 re-function relationship of (Pl)EctA by site-directed mutagenesis.

4 the binding interface were verified by site-directed mutagenesis.

5 annel outer vestibule experimentally by site-directed mutagenesis.

6 n transfer difference NMR (STD-NMR) and site-directed mutagenesis.

7 in E coli supports efficient multiplex site-directed mutagenesis.

8 substituted by a cysteine codon through site-directed mutagenesis.

9 uch as targeted genome modification and site-directed mutagenesis.

10 ants on both allergens was performed by site-directed mutagenesis.

11 of these binding sites was confirmed by site-directed mutagenesis.

12 site in the ion pore that we confirm by site-directed mutagenesis.

13 ype 2a-derived HCV clone (Jc1Gluc2A) by site-directed mutagenesis.

14 ort signal, and could be inactivated by site-directed mutagenesis.

15 with native mass spectrometry (MS) and site-directed mutagenesis.

16 CAT2 promoter activity was analyzed by site directed mutagenesis.

17 , adding a dynamic dimension to protein site-directed mutagenesis.

18 atalytic residues were identified using site-directed mutagenesis.

19 te those aspects of protein function through directed mutagenesis.

20 e determinants were resolved further by site-directed mutagenesis.

21 ome of these residues were probed using site-directed mutagenesis.

22 nction and validated six of these using site-directed mutagenesis.

23 isassociated to varying extents through site-directed mutagenesis.

24 ino acids critical for Opi3 activity by site-directed mutagenesis.

25 ns, kinetic studies, and the results of site-directed mutagenesis.

26 re, we identified catalytic residues by site-directed mutagenesis.

27 as the AdoMet-binding motif (FXGXG) by site-directed mutagenesis abolished the catalytic activity of

28 n and hydrolysis using a combination of site-directed mutagenesis, activity measurements using synthe

29 Here, employing site-directed mutagenesis, allelic exchange, quantitative PCR

30 2 intermediates and iterative rounds of site-directed mutagenesis allowed us to identify 4 of 413 res

31 a genetically encoded peptide scaffold, site-directed mutagenesis allows for rapid generation of anal

32 data and a catalytic model supported by site-directed mutagenesis allows full comparison with dicot C

33 Site-directed mutagenesis allows the generation of novel DNA

34 this study, using purified proteins and site-directed mutagenesis, along with fluorescence polarizati

35 Kinetic, molecular docking, and site-directed mutagenesis analyses confirmed these compounds

36 We have carried out an extensive site-directed mutagenesis analysis of the CD16A receptor comp

37 Using site directed mutagenesis and a site-specific phospho-antibod

38 Using site-directed mutagenesis and a specific in vitro transcripti

39 Using domain truncations, site-directed mutagenesis and an unbiased forward genetic scr

40 ermore, transcriptional start analysis, site-directed mutagenesis and bacterial two-hybrid interactio

41 quantum and classical simulations with site-directed mutagenesis and biophysical experiments, we sho

42 relocated by pressure was validated by site-directed mutagenesis and by inhibition by small peptides

43 Results from site-directed mutagenesis and competitive ligand-binding anal

44 ivity-based protein profiling utilizing site-directed mutagenesis and computational modeling.

45 rate that ChABC can be stabilized using site-directed mutagenesis and covalent modification with poly

46 In this report, combining single site-directed mutagenesis and double mutant analyses, we cond

47 lumen, which could both be confirmed by site-directed mutagenesis and electrophysiology.

48 ing with biochemical validation through site-directed mutagenesis and enzyme activity assays, we demo

49 imulations were performed together with site-directed mutagenesis and enzyme kinetic studies to ident

50 The prediction was validated by site-directed mutagenesis and evaluation of the relative liga

51 acids, which can be altered readily by site-directed mutagenesis and expressed in vitro and in vivo,

52 Through site-directed mutagenesis and functional analysis, we found t

53 Structural analysis combined with site-directed mutagenesis and functional studies inform on su

54 ve binding sites of C3G and GSH through site-directed mutagenesis and functional studies.

55 Site-directed mutagenesis and gene rescue studies show that A

56 leus of HTB-9 cells, as demonstrated by site-directed mutagenesis and green fluorescent protein (GFP)

57 Site-directed mutagenesis and homology models show the import

58 the structure, and verified them using site-directed mutagenesis and in vitro hormone-production ass

59 of these putative glycosylation sites, site-directed mutagenesis and lectin affinity purification id

60 Site-directed mutagenesis and mass spectrometry experiments s

61 olled proteolysis, gel electrophoresis, site-directed mutagenesis and microsecond MD simulations.

62 sh and human MR coupled with reciprocal site-directed mutagenesis and molecular dynamic (MD) simulati

63 ate and product complexes together with site-directed mutagenesis and molecular dynamics simulations

64 cco (Nicotiana tabacum L.) using CRISPR site-directed mutagenesis and overexpression assays.

65 Here we used site-directed mutagenesis and patch-clamp recordings to inves

66 We used site-directed mutagenesis and patch-clamp recordings to probe

67 Using site-directed mutagenesis and phosphorylation analysis of Pah

68 reinhardtii (CrHydA1) we have conducted site-directed mutagenesis and protein film electrochemistry t

69 The structure is confirmed by site-directed mutagenesis and provides a molecular framework

70 We used site-directed mutagenesis and single-cell patch-clamp to anal

71 We used site-directed mutagenesis and single-cell patch-clamp to anal

72 Here, using site-directed mutagenesis and stopped-flow kinetics, we exami

73 Site-directed mutagenesis and structural modeling analyses di

74 By site-directed mutagenesis and structure-guided analyses, we d

75 lecular mechanism that was supported by site-directed mutagenesis and targeted molecular dynamics sim

76 erstood, we used its structure to guide site-directed mutagenesis and to dissect its function.

77 ochondrial function, as demonstrated by site-directed mutagenesis and use of STAT3 knockout and mitoc

78 Through NMR studies, site-directed mutagenesis, and advanced molecular dynamics si

79 h tryptophan fluorescence spectroscopy, site-directed mutagenesis, and antibiotic susceptibility assa

80 gh a combination of SPOT peptide array, site-directed mutagenesis, and bio-layer interferometry, we i

81 with the help of isotopic labeling and site-directed mutagenesis, and complemented by integration an

82 assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzym

83 used the chemical modification of Trp9, site-directed mutagenesis, and crystallographic and computati

84 logy modeling, coevolutionary analyses, site-directed mutagenesis, and electrophysiology to examine t

85 ology protein modeling, ligand docking, site-directed mutagenesis, and electrophysiology, we show tha

86 icularly strong interactions, performed site-directed mutagenesis, and expressed the mutants in CHO c

87 ethods including chemical modification, site-directed mutagenesis, and fluorescent spectroscopy, we d

88 , we carried out in silico analysis and site-directed mutagenesis, and found a new sialic acid-bindin

89 ping was performed by pepscan analysis, site-directed mutagenesis, and hydrogen/deuterium exchange-ma

90 crystal structures, molecular docking, site-directed mutagenesis, and kinetic and thermodynamic anal

91 , were deduced from crystal structures, site-directed mutagenesis, and kinetic and thermodynamic anal

92 of Tg for SPCA1a by in silico modeling, site-directed mutagenesis, and measuring the potency of a ser

93 quantitative polymerase chain reaction, site-directed mutagenesis, and micrococcal nuclease assay to

94 tion of NMR, fluorescence spectroscopy, site-directed mutagenesis, and thermodynamics to elucidate th

95 relationships, using electrophysiology, site-directed mutagenesis, and voltage-clamp fluorometry.

96 Remarkably, biophysical approaches, site-directed mutagenesis, and X-ray crystallography uncovere

97 Using MS analysis, site-directed mutagenesis, and X-ray structural data analysis

98 hippocampus, together with an in vitro site-directed mutagenesis approach, we identify loss of STAT3

99 l for stabilizing mutp53(R175H) using a site-directed mutagenesis approach.

100 To overcome the limitations of existing site-directed mutagenesis approaches, we explored in vivo DNA

101 ze exclusion chromatography, as well as site-directed mutagenesis approaches, we show that the ACT do

102 emical labeling, mass spectrometry, and site-directed mutagenesis approaches, we show that thiram (an

103 a unique cysteine residue introduced by site-directed mutagenesis) as free-radical trapping 'tags' fo

104 pinpointed by computational results and site-directed mutagenesis at Ala33.

105 iple DNA fragments and did simultaneous site-directed mutagenesis at multiple sites.

106 ermined by cryo-electron microscopy and site-directed mutagenesis at the minus beta2 side converge in

107 ic analysis of Der p 2 was performed by site-directed mutagenesis based on the x-ray crystal structur

108 Through site-directed mutagenesis, based on a modeled structure, muta

109 In this study, using site-directed mutagenesis, biochemical and biophysical tools,

110 A combination of site-directed mutagenesis, biochemical and electrophysiologic

111 By combining site-directed mutagenesis, biochemical assays, and spectrosco

112 identity preservation system for products of directed mutagenesis can be established, the deviation r

113 We performed site-directed mutagenesis, cellular localization experiments,

114 s, NMR titrations, DNA-binding studies, site-directed mutagenesis, charge distribution, and sequence

115 re, using several approaches, including site-directed mutagenesis, Click O-GlcNAc labeling, immunoblo

116 rated with X-ray co-crystallography and site-directed mutagenesis, collectively reveal the structural

117 KEY MESSAGE: Successful site-directed mutagenesis combined with in silico modeling an

118 Truncation mutations and site-directed mutagenesis confirmed that the KH domain in DDX

119 Site-directed mutagenesis confirmed the essential role that t

120 The molecular dynamics simulation and site-directed mutagenesis confirmed the important roles of th

121 Site-directed mutagenesis confirms the observed interaction i

122 mutations into a wt infectious clone by site-directed mutagenesis considerably reduced replication.

123 We used site-directed mutagenesis coupled with direct measures of com

124 Using a combination of site-directed mutagenesis, DA-uptake, and cross-linking exper

125 ive structure-activity relationship and site-directed mutagenesis data facilitates the prediction of

126 Experimental validation is by in-vitro, site-directed mutagenesis data found in literature.

127 Site-directed mutagenesis data provides an invaluable complem

128 Site-directed mutagenesis demonstrated that substitution at a

129 Furthermore, site-directed mutagenesis determined that C61, C69, C137, and

130 2B enzymes using X-ray crystallography, site-directed mutagenesis, deuterium-exchange MS, isothermal

131 nteractions to validation using data on site-directed mutagenesis' effect on capsid assembly.

132 tails of IDP-SNP affinity profiles, and site-directed mutagenesis effects with a spatial resolution a

133 tudy, structural analyses combined with site-directed mutagenesis efficiently improved the functional

134 In this study, using COS-7 cells, site-directed mutagenesis, electrophysiological measurements,

135 Using a combination of site-directed mutagenesis, electrophysiology, and modeling to

136 y, cryo-EM, and CD analyses, along with site-directed mutagenesis, enzymatic assays, and molecular dy

137 al energies and molecular dynamics, and site-directed mutagenesis establish the mechanism of this tra

138 nd antigenic properties, we carried out site-directed mutagenesis experiments at the single-amino-aci

139 ntial interaction sites for PI(4,5)P(2) Site-directed mutagenesis experiments confirmed that the side

140 cysteines within the mature peptide and site-directed mutagenesis experiments demonstrated that the f

141 catalysis are discussed and compared to site-directed mutagenesis experiments.

142 ge coupling effects and predictions for site-directed mutagenesis experiments.

143 , using recombinant protein expression, site-directed mutagenesis, fluorimetric and biochemical assay

144 Here, we used site-directed mutagenesis, glutathione S-transferase pulldown

145 Here, using site-directed mutagenesis, GTP hydrolysis assays, coimmunopre

146 -driven hENT3 nucleoside transport with site-directed mutagenesis, homology modeling, and [(3)H]adeno

147 In silico modeling and site-directed mutagenesis identified charged residues at the

148 nd computational analysis together with site-directed mutagenesis identified five basic residues dist

149 Systematic site-directed mutagenesis identified the core amino acid resi

150 using an array of techniques, including site-directed mutagenesis, immunoblotting, FRET, and proximit

151 Molecular modeling and site-directed mutagenesis implicate several residues around t

152 , we addressed these questions by using site-directed mutagenesis in combination with enzymatic, tran

153 Combining brain slice studies and site-directed mutagenesis in HEK293T cells, we found that coc

154 , we targeted individual amino acids by site-directed mutagenesis in the [FeFe]-hydrogenase CpI of Cl

155 le dysfunctions related to mutations or site-directed mutagenesis in titin that alter the I-band stif

156 ilization of the coiled-coil domains by site-directed mutagenesis increases the effective diffusivity

157 Computational modeling and site-directed mutagenesis indicated that the mode of fatty ac

158 Site-directed mutagenesis indicates that tyrosine 645 in this

159 Combined with in silico modeling, site-directed mutagenesis, inhibition experiments and cell-ba

160 Site directed mutagenesis is widely used to understand the st

161 y, using statistical coupling analysis, site-directed mutagenesis, isothermal calorimetry, small-angl

162 Using site-directed mutagenesis, kinetic assays, and quantitative m

163 By site-directed mutagenesis, L249P was identified as the critic

164 from the N terminus from Thr to Asn by site-directed mutagenesis, making it constitutively inactive)

165 ed multiplex mutagenesis, over existing site-directed mutagenesis methods such as recombineering (rec

166 Compared with alternative site-directed mutagenesis methods, our protocol requires less

167 Here, we combine electrochemistry, site-directed mutagenesis, molecular dynamics and quantum che

168 Here, using site-directed mutagenesis, molecular modeling, and in cell-fr

169 Site-directed mutagenesis near either FAD produced altered yi

170 Furthermore, site-directed mutagenesis of a predicted ETS-binding site wit

171 Molecular modeling and site-directed mutagenesis of ARH3 revealed that numerous resi

172 netic circuits and biosynthetic pathways via directed mutagenesis of bacterial chromosomes.

173 Site-directed mutagenesis of both cofilin and actin revealed

174 the redox state of FAD4 or PRXQ through site-directed mutagenesis of conserved cysteine residues impa

175 nance (DEER) with functional assays and site-directed mutagenesis of conserved residues to illuminate

176 spectroscopy, heme quantification, and site-directed mutagenesis of histidine residues, we demonstra

177 alysis of PcUP1 with bound ligands, and site-directed mutagenesis of key residues provide additional

178 Site-directed mutagenesis of lsBSH demonstrated that Cys2 and

179 Site directed mutagenesis of lysine 68 to glutamine (K68Q), m

180 Site-directed mutagenesis of NEM1 and SPO7, coupled with phos

181 structure of the AT1R was used to guide site-directed mutagenesis of outward-facing hydrophobic resid

182 Site-directed mutagenesis of phosphorylated residues revealed

183 ture and function of CHT7, we performed site-directed mutagenesis of previously identified phosphoryl

184 e models such as interpreting/designing site-directed mutagenesis of proteins.

185 Site-directed mutagenesis of PxaTPS8 revealed several catalyt

186 Site-directed mutagenesis of RadH was used to identify cataly

187 Site-directed mutagenesis of RCAR1 showed that its tyrosine r

188 Furthermore, by site-directed mutagenesis of residues at the trimerization in

189 Using site-directed mutagenesis of residues in both proteins, coupl

190 Site-directed mutagenesis of residues near the predicted NAD(

191 encoded by CHO1 Truncation analysis and site-directed mutagenesis of the CHO1 promoter indicated that

192 -terminal receptor peptides followed by site-directed mutagenesis of the cleavage sites links recepto

193 findings is validated experimentally by site-directed mutagenesis of the key CcmK2 residue Serine 39.

194 Further, site directed mutagenesis of the miR-134 binding site in Sabi

195 Here, using site-directed mutagenesis of the mitochondrial COX1 gene from

196 Site-directed mutagenesis of the phytaspase cleavage sites in

197 Site-directed mutagenesis of the Sp1-binding site within the

198 By exploiting site-directed mutagenesis of the specific amino acids we iden

199 ct the activity of G. lovleyi NrfA, and site-directed mutagenesis of this arginine reduced enzymatic

200 Site-directed mutagenesis of Thr-48 to Ala (T48A) to prevent

201 ts, the use of amino acid blockers, and site-directed mutagenesis of UCP1, we propose a mechanism of

202 tallography, in vitro enzyme assays and site-directed mutagenesis, of the bacillaene synthase dehydra

203 Here, we performed site-directed mutagenesis on five envelope (E) protein amino

204 In this study, we made site-directed mutagenesis on the surface-exposed hydrophobic

205 Using site-directed mutagenesis, optical trap-based force spectrosc

206 of truncated MT2 that were generated by site-directed mutagenesis or Gibson assembly master mix, and

207 Past studies, based on site-directed mutagenesis or solvatochromism of the isolated

208 in vitro and rat in situ preparations, site-directed mutagenesis, patch-clamp, nerve recordings and

209 cosyltransferase structural biology and site-directed mutagenesis, pathway engineering, and deoxysuga

210 Site-directed mutagenesis, protein biochemical, and structura

211 Using site-directed mutagenesis, purified recombinant proteins, mam

212 its implications for heme transfer via site-directed mutagenesis, resonance Raman (RR), hydrogen-deu

213 Site directed mutagenesis results show that residues in the c

214 s by top-down MS, NMR spectroscopy, and site-directed mutagenesis revealed specific and well-conserve

215 ecular modeling/simulation coupled with site-directed mutagenesis revealed that Asp(147) or Asn(169)

216 Site-directed mutagenesis revealed that GRK2 Ser-685 phosphor

217 Site-directed mutagenesis revealed that Leu(46) and Phe(123)

218 hole genome sequencing, variant analyses and directed mutagenesis revealed that the heteroresistance

219 Site-directed mutagenesis revealed that the McpM precursor pe

220 at 1.94 angstrom resolution, along with site-directed mutagenesis, revealed how the active site poten

221 ected patients (n = 168), together with site-directed mutagenesis, revealed Nef position 9 as a subty

222 Site-directed mutagenesis reveals that this interaction promo

223 l and biochemical approaches, including site-directed mutagenesis, reverse genetics-based virus recov

224 Mass spectrometry and site-directed mutagenesis showed that chemically distinct HNO

225 Site-directed mutagenesis showed that Ser-491 is essential fo

226 Site-directed mutagenesis showed that the relevant sites for

227 using several approaches, including MS, site-directed mutagenesis, siRNA-mediated gene silencing, and

228 Site-directed mutagenesis studies revealed four substitutions

229 bauer spectroscopy measurements and the site-directed mutagenesis studies show that the red Fur prote

230 Extensive site-directed mutagenesis studies supported the importance of

231 Site-directed mutagenesis studies were consistent with bisubs

232 In this study, using computational and site-directed mutagenesis studies, we demonstrate the presenc

233 ed in silico hA(3)AR-homology model and site-directed mutagenesis study was performed to demonstrate

234 E15A and variants in which we, by using site-directed mutagenesis, substituted residues suggested to

235 Mechanistic studies using site-directed mutagenesis suggest that, following initial enz

236 Site-directed mutagenesis supports a model in which adenosine

237 s using antisense oligonucleotides or CRISPR-directed mutagenesis suppresses tumour growth.

238 Using site-directed mutagenesis, surface plasmon resonance, and cry

239 Site-directed mutagenesis targeting these tyrosine residues r

240 cDNAs were constructed by site-directed mutagenesis that encode PORB mutant proteins wi

241 Site-directed mutagenesis that swapped residue 153 between UG

242 Furthermore, we show by site-directed mutagenesis that tyrosine (Y382-384) within the

243 Site-directed mutagenesis then revealed that a previously uni

244 Site-directed mutagenesis to block HspB1 phosphorylation inhi

245 CD2AP, and phospho-CD2AP antibodies and site-directed mutagenesis to define the specific phosphorylat

246 in MGAT4D-L transfectants, we performed site-directed mutagenesis to determine requirements for MGAT1

247 SagS domain was used as constructs and site-directed mutagenesis to elucidate how SagS performs its

248 ferent biological kingdoms and employed site-directed mutagenesis to elucidate the molecular basis fo

249 Here, we have made use of site-directed mutagenesis to examine the contribution of subu

250 hes for candidate exosites, we utilized site-directed mutagenesis to identify a new exosite in caspas

251 with high-nanomolar affinity, and used site-directed mutagenesis to identify a patch of conserved re

252 In the current study, we use site-directed mutagenesis to identify K(100) and K(188) as ma

253 onstructs, bioinformatics analysis, and site-directed mutagenesis to identify Lon domains and residue

254 type fructooligosaccharide and utilized site-directed mutagenesis to identify residues involved in su

255 omplex, we used truncation variants and site-directed mutagenesis to investigate domains and residues

256 In this report we used site-directed mutagenesis to substitute specific amino acid r

257 Using site-directed mutagenesis to test multiple potential sites of

258 We used X-ray crystallography and site-directed mutagenesis to unravel the molecular and struct

259 R) spectroscopic methods, combined with site-directed mutagenesis, to determine the mechanism of a si

260 r, and validated this interface through site-directed mutagenesis together with functional and cell d

261 fect on STAT4 function was evaluated by site-directed mutagenesis using a lymphoblastoid B cell line

262 Site-directed mutagenesis was performed by partial gene synth

263 Site-directed mutagenesis was subsequently used to identify k

264 Site-directed mutagenesis was used to create orthogonal MTs p

265 i enzyme alpha2,6-sialyltransferase and site-directed mutagenesis was used to exchange individual ami

266 Site-directed mutagenesis was used to investigate the relativ

267 Site-directed mutagenesis was used to show that the NADH:quin

268 Site-directed mutagenesis was utilized to assess the role ind

269 Using site-directed mutagenesis, we confirmed that the predicted pu

270 Using truncation mutants and site-directed mutagenesis, we define the inhibitory face of t

271 Using site-directed mutagenesis, we demonstrate that the M159I subs

272 Through site-directed mutagenesis, we demonstrate that these residues

273 Using site-directed mutagenesis, we demonstrated that the conserved

274 Using site-directed mutagenesis, we determined that glycosylation o

275 ce alignment, pH-activity profiles, and site-directed mutagenesis, we evaluated a series of active si

276 esonance energy transfer technique, and site-directed mutagenesis, we examined the domains involved i

277 purified and recombinant proteins, and site-directed mutagenesis, we examined the role of a putative

278 Using site-directed mutagenesis, we found that mutations predicted

279 crum." While validating the model using site-directed mutagenesis, we found that the Tyr-542 residue

280 Using site-directed mutagenesis, we further defined this additional

281 Using site-directed mutagenesis, we generated FOXM1 mutant proteins

282 combination of in silico prediction and site-directed mutagenesis, we have mapped an exosite to a non

283 homology modeling of ENaC structure and site-directed mutagenesis, we identified a cleft region withi

284 Here, by using site-directed mutagenesis, we identified Arg-8 of subunit e a

285 Using in silico docking and site-directed mutagenesis, we identified Asp(230) in the extr

286 Using site-directed mutagenesis, we identified serine-465 as the si

287 In addition, using site-directed mutagenesis, we identify those amino acids that

288 Through extensive site-directed mutagenesis, we map phosphorylation to the Env7

289 Using site-directed mutagenesis, we mutated the specific sites of t

290 teraction and kinetic assays as well as site-directed mutagenesis, we provide further insight into th

291 g protein-lipid co-flotation assays and site-directed mutagenesis, we report that the N-region of the

292 ormatics-based approaches combined with site-directed mutagenesis, we show here that SLFN11 is phosph

293 es, molecular dynamics simulations, and site-directed mutagenesis, we show that 8-NBD-cGMP inhibits P

294 Here, using site-directed mutagenesis, we show that Asn-26 in the motif i

295 Through site-directed mutagenesis, we show that three amino acids wit

296 Using site-directed mutagenesis, we validated those surfaces in vit

297 inally, in vitro PLK1 kinase assays and site-directed mutagenesis were employed to demonstrate that H

298 ellular localization were identified by site-directed mutagenesis, which identified serine residues 4

299 the transition state model, we combined site-directed mutagenesis with kinetic experiments, yielding

300 y lineage analysis and structure-guided site-directed mutagenesis with large-scale functional signali