ライフサイエンスコーパス: directed mutation

1 ymes where key residues were altered by site-directed mutation.

2 combination of cloning, complementation and directed mutation.

3 latory and catalytic phenomena by studies in directed mutation.

4 of Asp-40 of VCAM-1 through the use of site-directed mutations.

5 was investigated through a series of target-directed mutations.

6 e (PhoA) fusions, precise deletions and site-directed mutations.

7 ly control gene expression or to induce site-directed mutations.

8 in DNA translocation activity caused by site-directed mutations.

9 null allele and Trbl transgenes bearing site-directed mutations.

10 pressing A beta class II molecules with site-directed mutations.

11 ent viruses were confirmed by analyzing site-directed mutations.

12 amples, and (iii) env clones containing site-directed mutations.

13 78-to-Ala (T78A) or Thr78-to-Ser (T78S) site-directed mutations.

14 Additional site-directed mutations affecting this interval commonly impa

15 e results underscore the difficulty of using directed mutations alone as a means of mapping the activ

16 hat affects dimerization as shown by limited directed mutations along the transmembrane domains.

17 Site-directed mutations also revealed the electronic quenchin

18 In vivo deletion and site-directed mutation analyses confirm that one GATA element

19 n sites into two different peptides and site-directed mutation analyses of acylation sites, often ser

20 n sites into two different peptides and site directed mutation analyses of acylation sites.

21 Sequential deletion and site-directed mutation analyses of the VEGF promoter demonstr

22 promoter, gel mobility shift assays and site-directed mutation analyses suggest that the putative NF-

23 re, we present a series of deletion and site-directed mutation analyses to identify amino acids in RI

24 Site-directed mutation analysis identified Arg(205), which is

25 Sequential deletion and site-directed mutation analysis of the CD1 promoter have iden

26 Using site-directed mutation and with femtosecond resolution, we ex

27 Using an infectious clone, site-directed mutations and 3' untranslated region (3'UTR) ex

28 rved residues, we generated a series of site-directed mutations and analyzed their impact on AL1 func

29 spontaneous mutations as well as constructed directed mutations and deletions in ppsR.

30 UNX site (+526 to +508), as revealed by site-directed mutations and exogenous expression of RUNX fact

31 n and related ansamycins, a series of target-directed mutations and heterologous gene expressions wer

32 Here, we used site-directed mutations and molecular dynamic simulations to

33 Through a series of site-directed mutations and phage display selections, the seq

34 Site-directed mutations and small internal deletions made in

35 ates of random mutation, the specific stress-directed mutations are also enhanced.

36 of many nucleic acid enzymes, the first site-directed mutations are made at conserved acidic active r

37 A site-directed mutation at Lys-1016 significantly decreased WR

38 Site-directed mutations at Arg(662), Pro(667), and Pro(670) w

39 xygen of biotin has been conducted with site-directed mutations at Asn 23, Ser 27, and Tyr 43.

40 In PS II, the site-directed mutations at D1 His198 affect the P(+)--P-absor

41 il arrangement was disfavored by adding site-directed mutations at F241 and F243 in the CH2 domains,

42 previously reported making a number of site-directed mutations at five sites located in the cofactor

43 intermembrane loops and of a series of site-directed mutations at hamster MFT residues highly conser

44 Site-directed mutations at multiple positions along the RNA-b

45 unction of Ctr1 containing deletions or site-directed mutations at the C terminus indicates a structu

46 The site-directed mutations at the conserved amino acid residues

47 We constructed a series of site-directed mutations at the dimer interface, and tested th

48 By making site-directed mutations at these positions and analysing the

49 Site-directed mutations at these residues reduce the rate of

50 We have made site-directed mutations at these residues, measured thermodyn

51 g loss-of-function mutations of EF3 and site-directed mutations based on the homology of the edema fa

52 A structure-guided, site-directed mutation-based inquiry of the catalytic contrib

53 Site-directed mutations betaE197C, betaE197D, and betaE197S i

54 ionally, this approach enables not only site-directed mutations, but also the insertion of DNA strand

55 ricus strain 98/2, a popular strain for site-directed mutation by homologous recombination.

56 To test this possibility, site-directed mutations by alanine substitution were introduc

57 instability in Friedreich's Ataxia is a DNA-directed mutation caused by improper DNA structure at th

58 id-targeted RET/PTC1 mutants carrying a site-directed mutation changing tyrosine (Y) to phenylalanine

59 ylate a recombinant Rpg1 inactivated by site-directed mutation confirmed that Rpg1 autophosphorylatio

60 Assays and directed mutations confirmed this activity and showed th

61 scopic measurements in combination with site-directed mutations constitute one important source of in

62 Site-directed mutation constructs were expressed in Xenopus o

63 of the mathematical issues arising from the directed mutation controversy, thereby offering alternat

64 Structure-guided site-directed mutations converted SLAC1 into a SLAH2-like nit

65 Site-directed mutations D262C, D262H, D262N, and D262T were m

66 s encoding RBD1 protein truncations and site-directed mutations demonstrated that the TMH domain is e

67 In conclusion, a site-directed mutation designed to improve the stability of p

68 obacter sphaeroides are investigated by site-directed mutations designed to alter the electrostatic e

69 Introduction of site-directed mutations designed to disrupt the hydrophobic f

70 Some site-directed mutations did not exhibit suppressor activity a

71 nt with previous reports, we found that site-directed mutations disrupting the protein-carbohydrate i

72 Kcv-like channels will be used to guide site-directed mutations, either singularly or in combination,

73 ion of recombinant viruses containing a site-directed mutation, encoding an amino acid substitution,

74 The results of site-directed mutation experiments established that icIL-1RaI

75 wealth of functional data generated by site-directed mutation experiments.

76 Isl-1 in overexpression, knockdown, and site-directed mutation experiments.

77 Furthermore, analysis of site-directed mutations found two putative Mg(2+)-binding res

78 The site-directed mutations H82A and Q222A (residues near the hem

79 In this study, site-directed mutations have been introduced to the human CSB

80 lar, we explored the effects that these site-directed mutations have over the enzyme kinetics with va

81 of new evidence that supposedly supports the directed mutation hypothesis has accumulated.

82 Within the latter region, site-directed mutations identified a consensus sequence "acac

83 These site-directed mutations identified a novel negatively charged

84 establish an example of transposon-mediated directed mutation, identify the protein responsible and

85 A site-directed mutation in a putative gene that resides downst

86 ntation studies using LtgG containing a site directed mutation in aspartate 343, confirmed the essent

87 cted COH1-GT2, a strain with a nonpolar site-directed mutation in glnQ.

88 f CS-B-null cells and mutant cells with site-directed mutation in the motif VI of the putative helica

89 but not with an orf4 gene containing a site-directed mutation in the putative zinc metalloprotease m

90 ze the peptidyl transferase activity of site-directed mutations in 23S rRNA at positions G2252, U2506

91 (EAL) from Salmonella typhimurium with site-directed mutations in a conserved arginine residue (R160

92 activation of ARD1 activity was tested using directed mutations in a loop near the substrate-binding

93 Site-directed mutations in BadR and the presence of phosphory

94 Using a series of site-directed mutations in both the genomic and cDNA context,

95 In this study, we generated a series of site-directed mutations in conserved sequence and structural

96 These are the first site-directed mutations in CP 43 to show such an effect.

97 Naturally occurring variants and directed mutations in DaaE have been generated and analy

98 Site-directed mutations in GIV's GEF motif disrupt its abilit

99 Site-directed mutations in irp6A, a gene previously reported

100 Site-directed mutations in its putative dimerization interfac

101 is approach promises to permit generation of directed mutations in many types of cells and organisms.

102 f1 interaction surface on Rpo41, making site-directed mutations in Rpo41 at positions predicted to re

103 Using site-directed mutations in SP-A, we determined that both the

104 Moreover, site-directed mutations in specific Ikaros-binding sites redu

105 l cis-acting elements were selected for site-directed mutations in the activation domain.

106 dependent functions of VP1, we created site directed mutations in the B3 domain that disrupted DNA b

107 To explore this issue, we analyzed homology-directed mutations in the C-terminal, DNA-wrapping domai

108 is diminished in constructs containing site-directed mutations in the Cbfa1 site indicate that Cbfa1

109 Site-directed mutations in the conserved residues of the chro

110 f a defined series of p9 truncation and site-directed mutations in the context of a reference infecti

111 Analysis of site-directed mutations in the CSF-1R further suggests that a

112 Site-directed mutations in the cytoplasmic domains of betaPS

113 multiple bands, known as A states, and site-directed mutations in the distal pocket of myoglobin.

114 Furthermore, we demonstrate that site-directed mutations in the esc protein previously shown t

115 Spontaneous or directed mutations in the gene that encodes citrate synt

116 Homologous recombination can produce directed mutations in the genomes of a number of model o

117 Site-directed mutations in the IBS indicate a probable cis-ac

118 Site-directed mutations in the most highly conserved region o

119 We then created a series of site-directed mutations in the OccR-binding site.

120 tosystem I (PS I) is investigated using site-directed mutations in the phylloquinone (QK) and FX bind

121 Site-directed mutations in the pore-lining M2 helix allowed t

122 characterized VP6 activity by creating site-directed mutations in the putative functional helicase d

123 n contrast, the debilitating effects of site-directed mutations in the single nuclear localization si

124 We analyzed the effects of site-directed mutations in the SUC2 promoter of Saccharomyces

125 r-reporter genes with 5'-truncations or site-directed mutations in the TGA elements showed that both

126 Several site-directed mutations in the transposase abolished activity

127 erized the effects of combinatorial and site-directed mutations in the two beta-turns of peptostrepto

128 basis of this association, we generated site-directed mutations in the various domains of CD82 and us

129 competence-based method for generating site-directed mutations in the wild-type fimbriated A. actino

130 tes in U3 sequences of MCF 247 and that site-directed mutations in these sites greatly diminish this

131 Site-directed mutations in this pocket impaired intracellular

132 expressing Flag-tagged CCR2B containing site-directed mutations in this region, 21-26, including a co

133 863-nt region and serial deletions and site-directed mutations indicated that the most distal PPRE (

134 n the enzyme active site as a result of site-directed mutations, inhibitor binding, and changes in pH

135 l ATPase, we have introduced equivalent site-directed mutations into the ATPbinding motif of each RPT

136 Directed mutation is a proposed process that allows muta

137 e in the beta3 S-x(3)-A-x(3)-I motif by site-directed mutations is sufficient to induce alphavbeta3 b

138 etermined by escape mutant analysis and site-directed mutation, is located in a non-aqueous island.

139 Functional analyses of site-directed mutations (K68D, K68E, K68N, K68P, and K68R) in

140 Site-directed mutations made in ori and Asn10 of Rep protein

141 Here we describe a novel mechanism of directed mutation mediated by the transposon, IS5 in Esc

142 The site-directed mutations modify the protein environment of the

143 the "helical cap" by evaluating single site-directed mutations, namely, L29A, I32E, L35A, L35E, L35R

144 Therefore, we conducted site-directed mutation of 15 amino acid residues of NuoM and

145 Deletion of the kinase domain or site-directed mutation of a subset of known PKCtheta phosphor

146 Site-directed mutation of AKT at T430 and T479 decreased O-Gl

147 Site-directed mutation of amadoriase II (R112E, R114E) result

148 Site-directed mutation of each of these conserved residues in

149 Site-directed mutation of Glu92 to glutamine had a detrimenta

150 Site-directed mutation of His-309 to Asn and Asp-210 to Ala r

151 Site-directed mutation of His30 to alanine destroyed its phos

152 Site-directed mutation of individual nucleotides within these

153 Moreover, site-directed mutation of Leu-219, the conserved leucine resi

154 eventing the FAM83B/EGFR interaction by site-directed mutation of lysine 230 of FAM83B suppressed PLD

155 Site-directed mutation of lysine to glutamate at position 72

156 Directed mutation of P1 was used to locate regions that

157 Importantly, the site-directed mutation of raptor at one phosphorylation site,

158 However, site-directed mutation of sequences located between -35 and -

159 confer an aggregation phenotype, (iii) site-directed mutation of specific residues can abolish recep

160 Site-directed mutation of STAT binding sites on the COX-2 pro

161 Site-directed mutation of T91 to alanine abolished PKA-mediat

162 Site-directed mutation of the -533-, -759-, and -773-bp GREs

163 Site-directed mutation of the -564- to -558-bp sequence resul

164 armacologically, by leptomycin B, or by site-directed mutation of the ALX NES, impairs CD28-mediated

165 ther species, this study introduced the site-directed mutation of the ATPase motif II in the human CS

166 Site-directed mutation of the CRE-II element caused a marked

167 Directed mutation of these residues supported this regio

168 ket, and we had previously found that a site-directed mutation of this amino acid resulted in a loss

169 Site-directed mutation of this AP-1 binding site eliminated t

170 Site-directed mutation of this HRE substantially reduced the

171 47 to catalysis is investigated here by site-directed mutation of this residue to His (Y447H), the fi

172 Single site-directed mutation of this TonE site led to a 54 +/- 5% (

173 Site-directed mutation of three consensus PKA phosphorylation

174 Site-directed mutation of two putative NFAT binding sites bet

175 Analysis of nine site-directed mutations of -246/-99 region reveals that both

176 Alternative substrates and site-directed mutations of active-site residues are used to p

177 Site-directed mutations of both OCT-1 and CAAT motifs abrogat

178 Site-directed mutations of both OCT-1 and CAAT motifs substan

179 Site-directed mutations of conserved residues in the first RR

180 The effects of several other single site-directed mutations of conserved residues of HlyC on diff

181 The effects of single site-directed mutations of conserved residues of HlyC on diff

182 coli MG1655 to its derivatives carrying site-directed mutations of genes for enterobactin synthesis (

183 Specific site-directed mutations of individual N terminus residues wer

184 er for chemotaxis was demonstrated with site-directed mutations of key amino acid residues in C. test

185 Site-directed mutations of key residues within the hydrophobi

186 Site-directed mutations of neighboring conserved P1 residues

187 a series of other tumor-associated, and site directed mutations of NFKB2.

188 Additionally, site-directed mutations of particular amino acid residues in

189 n heat capacity by means of a series of site-directed mutations of PwTBP.

190 We show that directed mutations of residues at a particular corner of

191 We explore the use of site-directed mutations of scytalone dehydratase to study inh

192 Site-directed mutations of serine-303 and threonine-494, pote

193 deletions of halpha(1C)1b promoter and site-directed mutations of the 2 CREB binding cis-elements in

194 Site-directed mutations of the conserved C93, C109, C116 and

195 Site-directed mutations of the human insulin promoter mapped

196 Thus, site-directed mutations of the pocket and lid loop led to sig

197 Using site-directed mutations of the redox-active tyrosines at the

198 Site-directed mutations of the residues involved in recogniti

199 Through the combined strategies of site-directed mutations of these amino acid residues and cati

200 Site-directed mutations of this region did not affect TCL1 se

201 With the two rat isoforms, site-directed mutations of two locations within the lipid bin

202 Functional inactivation by site-directed mutations of two Smad binding sites on the RON

203 s new evidence of a secondary effect of site-directed mutation on a RC chromophore.

204 studied to investigate the effects of a site-directed mutation on bound substrate conformations.

205 f posttranscriptional modifications and site-directed mutations on both the structure and function of

206 ied to investigate the effects of these site-directed mutations on bound-substrate conformations.

207 assessed with regard to the effects of site-directed mutations on class I and II activities.

208 clear import, we analyzed the effect of site-directed mutations on IE1 subcellular distribution.

209 pA promoter by measuring the effects of site-directed mutations on promoter activity.

210 The effects of the site-directed mutations on residues within the active site of

211 ere assessed by studying the effects of site-directed mutations on the binding affinity of (125)I-ASI

212 distribution that could be abrogated by site-directed mutations or treatment with the nuclear export

213 However, site-directed mutations predicted to impair ATP binding or hy

214 on with other studies in crystallography and directed mutation, puts the phosphate regulatory site at

215 Site-directed mutations R177A and R177K in the gene encoding

216 eptide synthetase cores, as well as the site-directed mutations, resulted in derivatives that retaine

217 Considerations of a large number of site-directed mutations reveal that two distinct regions, nam

218 Analysis of site-directed mutations revealed stereochemical and volume-de

219 An analysis of site directed mutations revealed that chaperone dimerization

220 ysis of transposase proteins containing site-directed mutations revealed the importance of the conser

221 the basis of these results, a number of site-directed mutations (SDM) were designed that were intende

222 The site-directed mutations studied here enable the individual is

223 ular agonist was initially indicated by site-directed mutation studies and activating peptides derive

224 ains of hmGlu2 and hmGlu3 combined with site-directed mutation studies has clarified the underlying m

225 ed by earlier X-ray crystallography and site-directed mutation studies.

226 ied in the screen as well as additional site-directed mutations suggest that the mode of DNA recognit

227 A series of domain swap and site-directed mutations, supported by structural comparisons,

228 between permease orthologs, a series of site-directed mutations targeted at residues within the two c

229 Site-directed mutations targeting the N-deacetylase catalytic

230 In this study, we constructed four site-directed mutations that changed aspartate 205 to alanine

231 mants that express ESC proteins bearing site-directed mutations that disrupt ESC-E(Z) binding in vitr

232 Similarly, site-directed mutations that disrupt this site reduce promote

233 Additional site-directed mutations that disrupted ethylene binding in ye

234 Site-directed mutations that made the coupling sequences comp

235 mic caspase-3 cleavage with and without site-directed mutations that mimic phosphorylation at Thr231/

236 Removal of the Env aa from v-Rel or site-directed mutations that revert the three mutant residues

237 Site-directed mutations that strongly impair transport or tha

238 Using site-directed mutations that substitute minK and KCNE3 residu

239 Site-directed mutations that substitute Phe for Trp241 and Ty

240 Additional site-directed mutations that were predicted to further streng

241 beta, and, using chimeric analysis and site-directed mutations, that the central region of PP1c beta

242 n enzyme catalysis were investigated by site-directed mutation to alanine.

243 -159 was shown by deletion analysis and site-directed mutation to be critical for promoter activity.

244 of hexokinase I, specifically engineered by directed mutation to block dimerization, remains monomer

245 nt work, we constructed four individual site-directed mutations to change each of these two residues

246 emical denaturant, mechanical force and site-directed mutations to demonstrate the presence of multip

247 We then used site-directed mutations to identify directly repeated sequenc

248 In addition, we use site-directed mutations to identify the most important antibo

249 Site-directed mutations to individual cysteine residues in ea

250 proposed active site were subjected to site-directed mutations to investigate their possible functio

251 ical analysis of PLZF-BTB/POZ harboring site-directed mutations to probe structure-function relations

252 In this study, we used directed mutations to show that all six blades of the HC

253 The site-directed mutations, W352C, W352L, and W352A, exhibit an

254 en these paired nucleotides, one set of site-directed mutations was constructed at positions 690:697

255 ional activation of gltBDF, a series of site-directed mutations was generated.

256 engths from the visible to the UV and a site-directed mutation we unambiguously determined that the p

257 Using site-directed mutations we have examined the contributions of

258 bination with a series of deletions and site-directed mutations, we determined that the first 22 bp o

259 With various site-directed mutations, we identified all possible electron

260 f AAV2-AAV8 hybrids and the creation of site-directed mutations, we mapped the domain that directs th

261 om both hydrogen-deuterium exchange and site-directed mutations, we suggest structural differences be

262 Site-directed mutations were also created in specific surface

263 Site-directed mutations were also made at residues (Phe479 an

264 Site-directed mutations were constructed at Glu-23 to assess

265 Site-directed mutations were constructed to inactivate one or

266 An extensive series of deletion and site-directed mutations were evaluated for their ability to r

267 ocyanin/cytochrome c6 and the PSI core, site-directed mutations were generated in the luminal J loop

268 Nine site-directed mutations were introduced at residue 117, using

269 Site-directed mutations were introduced in motif III S5 regio

270 n Qin-induced oncogenic transformation, site directed mutations were introduced in the winged helix (

271 Site-directed mutations were introduced into each of these RR

272 Site-directed mutations were introduced into HA-tagged Gag to

273 For this purpose, site-directed mutations were introduced into the full-length

274 incorporation and nuclear localization, site-directed mutations were introduced into the vpx gene of

275 Random and site-directed mutations were introduced into this segment of

276 Site-directed mutations were introduced to replace D1-His198

277 obic nature of this portion of p6(Gag), site-directed mutations were made at the minor protease cleav

278 ns between 23S rRNA and aminoacyl tRNA, site-directed mutations were made at the universally conserve

279 tions between Rubisco activase and ATP, site-directed mutations were made at two species-invariant as

280 In this study, site-directed mutations were made in the carboxyl-terminal nu

281 resected the full D-domain; therefore, site directed mutations were made to attribute precise sequen

282 Site-directed mutations were made to the phosphate-binding lo

283 Site-directed mutations were made to the phosphate-binding lo

284 to elucidate the function of subunit E, site-directed mutations were performed at the amino terminus

285 Site-directed mutations were prepared in a series of residues

286 Site-directed mutations were produced in the distal segments

287 ys (denoted pathway 1) are sensitive to site-directed mutation, whereas residues in the other propose

288 ed the efficiency of DeGeRing to induce site-directed mutations with and without a phenotype in three

289 Moreover, a site-directed mutation within the PilZ domain of CelA, the ca

290 By using mutants with site-directed mutations within a predicted C-terminal process

291 s, performed with an extensive panel of site-directed mutations within each of the six kelch motifs,

292 ities), a series of 16 alleles carrying site-directed mutations within FAT1 were constructed and anal

293 Site-directed mutations within Ll.ltrB, either in the catalyt

294 insertionally inactivated construct or site-directed mutations within the active site, demonstrated

295 s of Swi6, we generated both random and site-directed mutations within the ankyrin repeat domain of S

296 We then created deletion and site-directed mutations within the conserved PI 3-kinase doma

297 this ectoenzyme, human CD39 was modified by directed mutations within these ACRs or by sequential de

298 Directed mutations within these so-called eIF4G homology

299 Ca(2+) concentrations demonstrate that site-directed mutations within this region interfere selectiv

300 Deletion and site-directed mutations within this region revealed that the