ライフサイエンスコーパス: discoidal

1 oE and prepare apoE containing reconstituted discoidal 1-palmitoyl-2-oleoyl-l-phosphatidylcholine (PO

2 and relative alignment of apoA-I monomers on discoidal (9.4 nm) reconstituted high density lipoprotei

3 rt the discovery of polymer nanodiscs, i.e., discoidal amphiphilic block copolymer membrane patches e

4 ose small HDLs (like reconstituted HDLs) are discoidal and composed of APOA1, cholesterol, and phosph

5 The r-HDL were found to be discoidal and in the size range of native HDL.

6 ular determinants for stabilization of model discoidal and plasma spherical HDL.

7 esidues 1-43 or 44-65 obviously discriminate discoidal and spherical reconstituted HDL particles desp

8 In both discoidal and spheroidal rHDL, DPPC containing r-HDL wer

9 ein (HDL), and the formation of spherical or discoidal apoE-containing HDL.

10 Discoidal apoE3-phospholipid complexes using a substitut

11 llipsoidal DSH particles rapidly collapse to discoidal bilayer structures.

12 combines with phospholipids to form similar discoidal bilayers and may prove to be superior to human

13 tate that rHDL particles are protein bounded discoidal bilayers.

14 ts indicated that each of the 2 molecules of discoidal bound apoA-I exists in multiple conformations

15 This result, which is not limited to model discoidal but also extends to plasma spherical HDL, help

16 The composition of the 100 A discoidal complex is approximately 5 [1-44]apoA-I and ap

17 all-atom molecular dynamics simulation on a discoidal complex made of 1-palmitoyl-2-oleoyl-sn-glycer

18 lexes formed under similar conditions: small discoidal complexes (approximately 3:1 weight ratio, app

19 ed at an initial 1:1 weight ratio and larger discoidal complexes (approximately 4.6:1 weight ratio, a

20 asma apoA-I to form two sizes of homogeneous discoidal complexes and thus may be responsible for apoA

21 Nascent HDL are discoidal complexes composed of a phospholipid bilayer s

22 All four discoidal complexes displayed similar abilities to remov

23 olution structural view of the peptide.lipid discoidal complexes formed by a class A amphipathic alph

24 s suggests that the kinetic stability of the discoidal complexes is dominated by the lipid-lipid rath

25 choline (DMPC) bilayer vesicles into smaller discoidal complexes is enhanced as a function of decreas

26 Thermal unfolding of discoidal complexes of apolipoprotein (apo) C-1 with dim

27 t ambient temperatures, protein oxidation in discoidal complexes promotes their remodeling into large

28 displayed lipid-binding abilities and formed discoidal complexes that were similar in major diameter

29 At higher ratios, discoidal complexes were shown to exist together with a

30 dimyristoylphosphatidylcholine vesicles into discoidal complexes with an efficiency similar to that o

31 At 1:1 DMPC:[1-44]apoA-I (w/w) ratio, discoidal complexes with composition approximately 4:1 (

32 es of dimyristoylphosphatidylcholine to form discoidal complexes with diameters in the range of 15-20

33 , the so-called globular domain of ApoA1, in discoidal complexes with phospholipids and increasing am

34 lphosphatidylcholine/pyrene-R61C/E255C/apoE4 discoidal complexes, pyrene excimer fluorescence emissio

35 e reconstituted into phospholipid-containing discoidal complexes.

36 ement of the deletion mutant proteins in the discoidal complexes.

37 econstituted high density lipoprotein (rHDL) discoidal complexes.

38 inal (GSDME-N) mRNA to induce pyroptosis and discoidal domain receptor 1 (DDR1) shRNA to suppress imm

39 uffling and a failure to acquire the typical discoidal erythroid shape but they can enucleate.

40 Discoidal forms of high density lipoproteins (HDL) are c

41 ein (apo) A-I, in spheres vs. better studied discoidal forms.

42 ch undergo regulated exocytosis of subapical discoidal/fusiform vesicles (DFV) during bladder filling

43 The discoidal/fusiform vesicles (DFV) of bladder umbrella ce

44 n bladder umbrella cells a subapical pool of discoidal/fusiform-shaped vesicles (DFVs) undergoes Rab1

45 by use of a bolus injection of reconstituted discoidal HDL (recHDL).

46 itatively with loss of LCAT activity in both discoidal HDL and HDL(3), the enzyme's physiological sub

47 ts corroborate our earlier analysis of model discoidal HDL and indicate that a kinetic mechanism prov

48 ed two general models of apoA-I structure in discoidal HDL complexes.

49 consistent with the destabilization of model discoidal HDL observed upon increasing the A-II to A-I r

50 l three monomers of apo A-I are bound to the discoidal HDL particle in a hairpin conformation.

51 4-243) wrapped around the circumference of a discoidal HDL particle.

52 hree monomers of apo A-I to a 150 A diameter discoidal HDL particle.

53 ts of apoA-I were analyzed in reconstituted, discoidal HDL particles composed of phospholipids contai

54 We examined the effects of the size of discoidal HDL particles containing wild-type (WT) apoA-I

55 phobic residues in the C-terminus, generated discoidal HDL particles indicating a defect in their con

56 In this work, discoidal HDL particles of different size were reconstit

57 binding to cells and the compositions of the discoidal HDL particles that are produced.

58 rt the belief that apo A-I binds to lipid in discoidal HDL particles via the belt conformation.

59 po) A-I in large (9.6 nm) and small (7.8 nm) discoidal HDL particles were determined by hydrogen-deut

60 are discussed for the binding of apo A-I to discoidal HDL particles with diameters identical to thos

61 made of a membrane-PL mixture and FC yields discoidal HDL particles with diameters in the range 9-17

62 Molecular belt models for discoidal HDL particles with differing diameters are pre

63 rp264Ala] contained mostly spherical and few discoidal HDL particles, and apoE4[Phe265Ala] contained

64 In terms of discoidal HDL particles, there has been a debate as to t

65 and "hairpin" models of apoA-I structure in discoidal HDL particles.

66 n HDL, we generated highly defined benchmark discoidal HDL particles.

67 gests the role of apoA-I in the formation of discoidal HDL particles.

68 ereas the apoA-I[Delta(89-99)] mutant formed discoidal HDL particles.

69 Discoidal HDL prepared with apoA-I containing a Met-148-

70 a relatively large boundary layer in smaller discoidal HDL promotes preferential distribution of phos

71 ts of salt, pH, and point mutations on model discoidal HDL reconstituted from human apolipoprotein C-

72 ed spherical HDL by incubating reconstituted discoidal HDL with physiological plasma-remodeling enzym

73 HDL species involving spontaneous fusion of discoidal HDL with spherical HDL and subsequent fission.

74 rface curvature during conversion of nascent discoidal HDL(A-I) and HDL(A-II) containing either apoA-

75 and carboxyl-terminal deletion mutant formed discoidal HDL, and a carboxyl-terminal deletion mutant f

76 ydrophobic when apoA-I was incorporated into discoidal HDL, and Tyr(192) of HDL-associated apoA-I was

77 we propose a detailed model for the smallest discoidal HDL, consisting of two apoA-I molecules wrappe

78 After attachment of LCAT to discoidal HDL, the helix 5/5 domains in apoA-I form amph

79 ion on the spatial organization of apoA-I in discoidal HDL, we engineered three separate cysteine mut

80 n (HDL) region and promoted the formation of discoidal HDL, whereas the apoE4-mut1 did not displace a

81 r the "double-belt" model of ApoE in nascent discoidal HDL-like lipoproteins, where two ApoE proteins

82 at least two sizes of relatively homogeneous discoidal HDL-like particles depending on the initial li

83 ith phospholipids to produce nanometer-scale discoidal HDL-like particles.

84 eas apoA-I[delta(1-41)delta(185-243)] formed discoidal HDL.

85 enhances net cholesterol efflux mediated by discoidal HDL.

86 sterol in VLDL and HDL, and the formation of discoidal HDL.

87 DL particles, and apoE4[Phe265Ala] contained discoidal HDL.

88 the structure, stability, and remodeling of discoidal HDLs reconstituted from human apolipoproteins

89 2-hydroxypropyl-beta-cyclodextrins and with discoidal high density lipoprotein (HDL) particles to pr

90 Apolipoprotein A-I (apoA-I) readily forms discoidal high density lipoprotein (HDL) particles with

91 The structure of apoA-I on discoidal high density lipoprotein (HDL) was studied usi

92 lations on a series of progressively smaller discoidal high density lipoprotein particles produced by

93 lano) and apo A-I(R151C)(Paris), to lipid in discoidal high-density lipoprotein (HDL) particles are p

94 ls for apolipoprotein A-I (apo A-I) bound to discoidal high-density lipoprotein (HDL) particles, base

95 l-atom model for apolipoprotein (apo) A-I in discoidal high-density lipoprotein in which two monomers

96 ave been carried out on three separate model discoidal high-density lipoprotein particles (HDL) conta

97 icles by apolipoprotein A-I (apoA-I) to form discoidal high-density lipoproteins (rHDL) was dramatica

98 LCAT by apolipoprotein (apo) A-I on nascent (discoidal) high-density lipoproteins (HDL) is essential

99 ganization of apolipoprotein A-I (apoA-I) in discoidal, high-density lipoprotein (HDL) complexes with

100 with the three full-length apoE isoforms are discoidal in shape, and structurally indistinguishable.

101 Predominately discoidal in shape, these particles have diameters betwe

102 that reconstituted apoA-I/HDL particles are discoidal in shape.

103 ituted apoA-I/HDL particles, in general, are discoidal in shape.

104 asma HDL and apoA-I levels and converted the discoidal into spherical HDL, indicating that the LCAT a

105 According to these four metrics, discoidal knapping appears to be easiest among the sampl

106 nts with LCAT deficiency have abnormal small discoidal LDLs and HDL particles, and develop kidney fai

107 They are discoidal lipid bilayer fragments encircled and stabiliz

108 (apo A-I) and its engineered constructs form discoidal lipid bilayers upon interaction with lipids in

109 ccumulates in the medium as apolipoprotein E-discoidal lipid particles.

110 Bicelles are bilayered discoidal lipid-detergent assemblies which are useful as

111 ly process, we prepared soluble monodisperse discoidal lipid/protein particles with controlled size a

112 o main competing models for the structure of discoidal lipoprotein A-I complexes both presume that th

113 complex provides a high-resolution view of a discoidal lipoprotein particle in which all of the inter

114 ive to the lipid bilayer was investigated in discoidal lipoprotein particles made with 1-palmitoyl-2-

115 a second apolipoprotein residing in the same discoidal lipoprotein.

116 id along the periphery of the bilayer of the discoidal lipoprotein.

117 olesterol efflux and esterification in model discoidal lipoproteins (including reduced protein size,

118 ts shows that protein oxidation destabilizes discoidal lipoproteins and accelerates protein unfolding

119 ine the number of apo A-I molecules bound to discoidal lipoproteins and compare this with values obta

120 re in solution facilitates reconstitution of discoidal lipoproteins but has no significant effect on

121 Using discoidal lipoproteins made with a combination of apolip

122 cture in protein-lipid interactions, we used discoidal lipoproteins reconstituted from dimyristoylpho

123 e lipoproteins as well as being able to form discoidal lipoproteins upon incubation with either lipos

124 fide form of the mutant was not able to form discoidal lipoproteins with liposomes of either dimiryst

125 on in the formation and kinetic stability of discoidal lipoproteins, thermal unfolding and refolding

126 mutant, as determined by its ability to form discoidal lipoproteins, was nearly identical to that of

127 by fluorescence resonance energy transfer in discoidal lipoproteins.

128 oLp-III with the phospholipid acyl chains in discoidal lipoproteins.

129 ApoE adopting antiparallel dimers in nascent discoidal lipoproteins.

130 as incorporated into soluble nanometer scale discoidal membrane bilayers (nanodiscs), and potentials

131 le of MHC clustering, we exploited nanoscale discoidal membrane mimetics (nanolipoprotein particles)

132 ew, exceptionally preserved specimens of the discoidal metazoan Rotadiscus grandis from the early Cam

133 teristic of plasma apolipoproteins and forms discoidal micelles with phosphatidylcholine.

134 s or native lipoproteins but interacted with discoidal micelles.

135 ins upon incubation with either liposomes or discoidal micelles.

136 The iNPG is a discoidal micrometer-sized particle that can be loaded w

137 GLAMs are hydrogel-based discoidal microparticles that adhere to macrophages with

138 s using v-SNARE-reconstituted 23-nm-diameter discoidal nanolipoprotein particles (vNLPs) as fusion pa

139 Nanodiscs are examples of discoidal nanoscale lipid-protein particles that have be

140 Nanodiscs are an example of discoidal nanoscale self-assembled lipid/protein particl

141 ormation of two populations of FC-containing discoidal nascent HDL particles.

142 ubilizes the exovesiculated domain to create discoidal nascent HDL particles.

143 Like 2F, 4F also forms discoidal nascent high density lipoprotein-like particle

144 The peptide Ac-18A-NH2 forms discoidal nascent high density lipoprotein-like particle

145 by 70% and 77% over the random models for a discoidal or an ellipsoidal stem cell confinement respec

146 l structure, independent of whether it forms discoidal or vesicular complexes.

147 olipoprotein AI (423:74:1 mol/mol) forming a discoidal particle 360 A in diameter and 45 A thick; the

148 stabilization of the ApoA1-POPC-cholesterol discoidal particle and allows for a more optimal lipid p

149 -I) and phospholipid (apoA-I/HDL) has been a discoidal particle approximately 100 A in diameter and t

150 edominantly high levels of apoA-I containing discoidal particles and had an increased prebeta1-HDL/al

151 able to transform phospholipid vesicles into discoidal particles but at a 3-fold reduced rate compare

152 ployed to self-assemble soluble monodisperse discoidal particles called Nanodiscs.

153 xpressed in ldlA-7 cells using reconstituted discoidal particles consisting of apoE, 1-palmitoyl-2-ol

154 macrophages, 2) apolipoprotein E only formed discoidal particles following macrophage cholesterol enr

155 lts indicate that the formation of apoE.DMPC discoidal particles occurs in a series of steps.

156 ested-motility states in ensembles of active discoidal particles powered by induced-charge electropho

157 n morphology (lamellar/vesicular and stacked discoidal particles reminiscent of those in lecithin/cho

158 can be overcome by cellular backpacks (BPs), discoidal particles that adhere on the macrophage surfac

159 oE with phospholipid and cholesterol to form discoidal particles that floated at densities of 1.08-1.

160 Adsorption of these discoidal particles to clean hydrophilic glass (or silic

161 imyristoylphosphatidylcholine (DMPC) to form discoidal particles was investigated by introducing sing

162 choline, can self-assemble into monodisperse discoidal particles with diameters <20 nm that transmigr

163 lipoproteins (lamellar/vesicular and stacked discoidal particles), occlusive coronary atherosclerosis

164 in size and morphology and included numerous discoidal particles, mimicking those observed in LCAT-de

165 ion with DMPC liposomes, and the size of the discoidal particles.

166 inside the pores of quasi-hemispherical and discoidal particles.

167 tent was somewhat less than in reconstituted discoidal PC.apoA-I complexes for all apoA-I variants, s

168 Conversion of discoidal phospholipid (PL)-rich high density lipoprotei

169 ccumulate within the hydrophobic core of the discoidal phospholipid bilayer transforming it into a sp

170 esteryl ester molecules in the middle of the discoidal phospholipid bilayer.

171 in the lipid-free state and in reconstituted discoidal phospholipid-cholesterol-apoA-I particles (rHD

172 olarized towards the antitumour phenotype by discoidal polymer micrometric 'patches' that adhere to t

173 A1 transporter function have only very small discoidal prebeta-1 HDL, and develop hepatosplenomegaly,

174 lity to transform phospholipid vesicles into discoidal protein-lipid complexes and that Thr-31 is a k

175 ing membrane patches in the form of nanosize discoidal proteolipid particles or "native nanodiscs." U

176 erase (LCAT), to form cholesteryl ester, the discoidal r-HDL became spheroidal.

177 In discoidal r-HDL, we found that POPC >/= DOPC = PAPC/DPPC

178 r-HDL were hydrolyzed at a faster rate than discoidal r-HDL.

179 choline (DMPC) binding kinetics, and size of discoidal reconstituted high-density lipoprotein (rHDL)

180 ion of rotational alignment was observed for discoidal red blood cells.

181 Discoidal rHDL particles containing two lipid-bound apoA

182 "fixed helix-helix registry." Additionally, discoidal rHDL were transformed in vitro to core-contain

183 These discoidal shaped cells underwent a dramatic form of acti

184 The structure showed a discoidal-shaped LDL particle with high-density regions

185 can convert multilamellar DMPC vesicles into discoidal-shaped particles.

186 (CD44TA) targeting moiety were conjugated to discoidal silicon mesoporous microparticles (SMP) to enh

187 antibody-labeled and energy-focusing porous discoidal silicon nanoparticles (nanodisks) and high-thr

188 roup protons yields the molecular weight and discoidal size, while the skewness of the NMR peaks in t

189 ssary to enable the efficient measurement of discoidal sizes of 2D polyaramids and tracking of their

190 l segments was incorporated into each of the discoidal species.

191 Lenticulatheca encompasses discoidal sporangia containing monads formed from dyads

192 Early studies of rHDL suggested a discoidal structure, which included pairs of antiparalle

193 al region of apoA-I binds lipid and can form discoidal structures and a heterogeneous population of v

194 ot perturb their ability to form homogeneous discoidal structures.

195 Conversion from a discoidal to a saddle-shaped particle involves loss of h

196 de that as lipid-bound apoA-I adjusts from a discoidal to a spherical surface its intermolecular inte

197 tion of high-density lipoproteins (HDL) from discoidal to spherical particles.

198 00 microm(2)), exocytosis of a population of discoidal vesicles located in the apical cytoplasm of th