ライフサイエンスコーパス: disulfide bridge

1 mutagenesis, in which the gap is locked by a disulfide bridge.

2 ow pH, proteolysis, and an intact interchain disulfide bridge.

3 paclitaxel (PTX) to vitamin E (VE) through a disulfide bridge.

4 mposed of two subunits linked by a conserved disulfide bridge.

5 zed, and its structure was stabilized with a disulfide bridge.

6 SDP is a 42-residue peptide with one disulfide bridge.

7 conformation with an engineered interdomain disulfide bridge.

8 sults in homodimerization of Src linked by a disulfide bridge.

9 subunits are glycosylated and connected by a disulfide bridge.

10 ified in positions 2, 3, and 7 and/or at the disulfide bridge.

11 as completely reversed upon reduction of the disulfide bridge.

12 utants reveals that the two cysteines form a disulfide bridge.

13 isulfide bonds and homodimerizes via another disulfide bridge.

14 during oxidation to form the Cys-2 to Cys-7 disulfide bridge.

15 ng conditions, confirming the existence of a disulfide bridge.

16 ed clamshell conformation by incorporating a disulfide bridge.

17 site Arg-122, and restoration of the missing disulfide bridge.

18 ed to be a homotetramer with an intersubunit disulfide bridge.

19 d Cu(I) ions over forming the intramolecular disulfide bridge.

20 ess 2 motifs related to beta-defensins and 6 disulfide bridges.

21 ble, as needed to design the state-selective disulfide bridges.

22 inally amidated peptide cross-linked by four disulfide bridges.

23 in backbone and aromatic residues as well as disulfide bridges.

24 ing agents due to the formation of nonnative disulfide bridges.

25 lin-like domains containing three intrachain disulfide bridges.

26 a 62-amino acid peptide cross-linked by five disulfide bridges.

27 ry structure and experimental constraints on disulfide bridges.

28 We discuss the biological relevance of such disulfide bridges.

29 5.3 kDa), with six cysteine residues forming disulfide bridges.

30 revealed an important structural role of the disulfide bridges.

31 sequence but also on the localization of the disulfide bridges.

32 loped, comprising 12-16 amino acids with two disulfide bridges.

33 oxin that blocks nAChRs and does not possess disulfide bridges.

34 was no evidence for intermolecular FLS2-FLS2 disulfide bridges.

35 sically disordered protein that can form two disulfide bridges.

36 tory C-terminal tail and stabilized by three disulfide bridges.

37 tion-pi interactions, but not intramolecular disulfide bridges.

38 of the seed protein both stabilized through disulfide bridges.

39 nterface of sfALR, close to the intersubunit disulfide bridges.

40 gh-molecular-weight complex held together by disulfide bridges.

41 ikely representing enzyme homodimers held by disulfide bridges.

42 red, including efficient mapping of multiple disulfide bridges.

43 ent collagen triple helix to form interchain disulfide bridges.

44 not be applicable when target proteins have disulfide bridges.

45 that is tethered to the C-terminal stalk by disulfide bridges.

46 ix core that is stabilized by four conserved disulfide bridges.

47 ly, fibrillation is robust to interchange of disulfide bridges.

48 nsulin, a two-chain protein containing three disulfide bridges.

49 particle formation by forming intermolecular disulfide bridges.

50 s, approximately 50% of which were linked by disulfide bridges.

51 three intramolecular and one intermolecular disulfide bridges.

52 receptor (TIR)-domain fold stabilized by two disulfide bridges.

53 e-rich trypsin inhibitor MCoTI-II with three disulfide bridges.

54 ntiparallel beta-strands stabilized by three disulfide bridges.

55 isting of two alpha1beta units, connected by disulfide bridges.

56 or seven different peptides with two to four disulfide bridges.

57 ith oligomerization in tetramers mediated by disulfide bridges.

58 influencing the positioning of cysteines and disulfide bridging.

59 eract with serotonin signaling pathways: the disulfide-bridged 2,5-diketopiperazine gliotoxin.

60 seven-transmembrane (TM) receptor conserved disulfide bridge (7TM bridge) linking transmembrane heli

61 of cysteine residues forming the C-terminal disulfide bridge abrogates pilus assembly, in turn elimi

62 ed a trapped dimer (dCXCL1) by introducing a disulfide bridge across the dimer interface.

63 LC translocation; premature reduction of the disulfide bridge after channel formation arrests translo

64 conformationally restrained by three or more disulfide bridges, although recent findings by several g

65 yldithio)ethanamine hydrochloride formed the disulfide bridge and provided the terminal amine group,

66 give discrete products while maintaining the disulfide bridge and thus peptide conformation.

67 generally basic peptides with three or four disulfide bridges and a molecular mass of ~5 kDa.

68 e binding cavity by a pair of closely spaced disulfide bridges and a short helical segment within the

69 te the importance of conserved extracellular disulfide bridges and aromatic residues in extracellular

70 nserved tertiary structure stabilized by two disulfide bridges and direct the migration of leukocytes

71 have employed structure-based engineering of disulfide bridges and native mass spectrometry to show t

72 hydrogen bonds, supplemented by two proposed disulfide bridges and receptor-ligand contacts, derived

73 e describe the precise connection of all the disulfide bridges and show that the IgG2 C H1 and C-term

74 with the formation of interchain, nonnative disulfide bridges and the establishment of molten globul

75 fide linkages, particularly, for intertwined disulfide bridges and the unexpected disulfide scramblin

76 he role played by previously uncharacterized disulfide-bridge and domain-swapped interfaces from crys

77 The two alpha-subunits are connected by a disulfide bridge, and both alpha- and beta-subunits are

78 Each CH3 monomer contains a conserved buried disulfide bridge, and we find that the successive reduct

79 as long as bovine chymotrypsinogen, has less disulfide bridges, and is a single polypeptide.

80 retro-translocates with oxidized intrachain disulfide bridges, and only upon proteasomal inhibition

81 salt bridges and side-chain hydrogen bonds, disulfide bridges, and propensities to form alpha-helice

82 te that all residues in this region can form disulfide bridges, and that the percentage of dimers inc

83 er Cys residues were found to be involved in disulfide bridges, and these are necessary for correct f

84 tural characterization of peptides including disulfide bridges annotation in the sequence.

85 loop 2 in conjunction with two extracellular disulfide bridges appeared to open and shape the entranc

86 Further, the disulfide bridge appears to prevent an inhibitory confor

87 rified this proximity by using an engineered disulfide bridge approach.

88 We find that disulfide bridges are abundant in connective and liver E

89 nce of the Cys-rich SMB domain, how its four disulfide bridges are arranged in the molecule remains h

90 pulsed light is an all-or-none process where disulfide bridges are broken and the enzyme is unfolded.

91 Disulfide bridges are commonly found covalent bonds that

92 osslinking in reducing conditions shows that disulfide bridges are involved in KCC2 dimerization.

93 Subsequently, reductant is removed and all disulfide bridges are reoxidized to reform covalent inte

94 tudy protein fibrillization, since its three disulfide bridges are retained in the fibrillar state an

95 These disulfide bridges are unique for the known homophilic in

96 energetically preferred; however, all eight disulfide bridge arrangements are essentially possible.

97 ree-stranded antiparallel beta-sheet and the disulfide bridge array typical of vertebrate beta-defens

98 ins and is shown to be fully compatible with disulfide bridges, as evidenced by the selective modific

99 e normally rigid intramolecular Cys57-Cys146 disulfide bridge assumed two conformations.

100 her with alternating homodimer formation via disulfide bridges at the C-terminal Fn tail, should lead

101 he thermal denaturation data point shows the disulfide bridges being responsible for the stability of

102 chains of NC2, a stable heterotrimer with a disulfide bridge between alpha1 and alpha3 chains is for

103 A disulfide bridge between C51a and C113, unique to the as

104 A double mutation that removes the disulfide bridge between C744 and C798 gives rise to gre

105 there is also evidence for an intramolecular disulfide bridge between consecutive cysteine residues.

106 ith mass spectrometry analysis showed that a disulfide bridge between Cys(499) and Cys(587) is centra

107 evidence suggests strongly that NO induces a disulfide bridge between Cys-110 and Cys-132 in intact c

108 The N and C termini are linked by a disulfide bridge between Cys1 and Cys158.

109 A disulfide bridge between Cys282 (the "x" of the SxN moti

110 The N-terminal loop, caused by a disulfide bridge between cysteines at positions 1 and 7,

111 loenzyme, required a covalent intermolecular disulfide bridge between GCLC and GCLM.

112 avage site (R508S/R511S) or by introducing a disulfide bridge between gp120 and gp41 designated "SOS"

113 referred to as class A carbapenemases, is a disulfide bridge between invariant Cys(69) and Cys(238)

114 r compatibility with collagen identifies the disulfide bridge between proximal homocysteine (Hcy) and

115 The peptide contains a disulfide bridge between residues two and seven and an a

116 The processing sites and a disulfide bridge between the 18- and 10-kDa chains could

117 on, whereas the chemokine receptor conserved disulfide bridge between the N terminus and TMVII is nee

118 (ECL)-2, chemokine receptors (CCR) contain a disulfide bridge between the N terminus and what previou

119 more stable version of cAb-HuL22 by adding a disulfide bridge between the two beta-sheets in the hydr

120 The disulfide bridge between the two chromophores is cleaved

121 the 7 transmembrane receptor (7TM)-conserved disulfide bridge between transmembrane (TM) helix 3 and

122 The protein also contained an intramolecular disulfide bridge between two cysteines (Cys) that are co

123 eine residue (Cys-298) in position to form a disulfide bridge between two Siglec-E polypeptides.

124 CHCH-domain containing subunits that contain disulfide bridges between CX9C motifs; they are processe

125 odimers and heterodimers with E1 mediated by disulfide bridges between cysteine residues.

126 activity through the reversible formation of disulfide bridges between cysteine thiols.

127 terlinked rings A and B are obtained through disulfide bridges between L-Cys3 and D-Cys8 and between

128 triple helix, and the cysteine residues form disulfide bridges between the different strands.

129 lar polymer system is prepared by complexing disulfide-bridged biguanidyl adamantine (Ad-SS-GD) with

130 forms of an immobilized NGB showed that the disulfide bridge both defines the kinetics of NO dioxyge

131 t all of the cysteine residues known to form disulfide bridges both within each monomer and between m

132 used on an antigenic peptide fragment from a disulfide bridge-bounded region spanning the V1 and V2 h

133 Because of this, ZmTrxh is unable to reduce disulfide bridges but possesses a strong molecular chape

134 It revealed the presence of four disulfide bridges, but no close structural analogs could

135 nking under reducing conditions that disrupt disulfide bridges, but soluble fibronectin did not.

136 eolysis and to reduction of its intersubunit disulfide bridges by glutathione.

137 the helix-loop-helix fold stabilized by two disulfide bridges C(1)-C(4) and C(2)-C(3).

138 Moreover, the intramolecular disulfide bridge (C23/45) of HMGB1 is required for bindi

139 deposited poly(allylmercaptan) surfaces via disulfide bridge chemistry and are found to readily unde

140 , state-selective molecular stapling through disulfide bridges, competition binding saturation transf

141 ve applied electrochemistry (EC) to overcome disulfide bridge complexity in top-down analysis of mAbs

142 Engineered disulfide bridges, computationally predicted to interfer

143 se of the LC from the HC by reduction of the disulfide bridge concomitant with LC refolding in the cy

144 In the conventional pattern, two disulfide bridges connect an alpha-helical segment to th

145 ses are characterized by specific interchain disulfide bridge connections.

146 ach for such mapping and apply it to a three-disulfide-bridged conotoxin, mu-SxIIIA (from the venom o

147 y selectivity and metabolic stability of the disulfide-bridge-containing peptide 1.

148 Here, we have used disulfide bridge cross-linking to test this hypothesis.

149 Disulfide bridge crosslinking demonstrates that SecA tra

150 Using disulfide bridge crosslinking, we have stabilized the E.

151 A disulfide-bridged cyclic RGD peptide, named iRGD (intern

152 amics and self-association by disrupting the disulfide bridge (Cys(3)-Cys(14)) in SST.

153 Intersubunit disulfide bridges, Cys-206-Cys-206 and Cys-243-Cys-243,

154 in each case contains the canonical A20-B19 disulfide bridge (cystines 18-61 in IGF-I and 19-85 in h

155 of C69G-GES-5 shows that two domains of this disulfide bridge-deficient enzyme are held together by a

156 the membrane this symmetry is broken by the disulfide-bridged dimerization of the extracellular Ig d

157 Rigidifying this contact permanently with a disulfide bridge disrupts ligand-induced receptor activa

158 The structure reveals a five-disulfide-bridged domain similar to CRDs of Frizzled pro

159 We propose that the formation of disulfide bridges drives membrane insertion of FGF2 olig

160 ircuit current were reduced, only one of the disulfide bridges eliminated the activity of the toxins

161 re, the rigid nature of this modification by disulfide bridging enables the successful detection of a

162 se ligand-binding clefts were locked shut by disulfide bridges engineered across lobes.

163 predicted by molecular modeling to disrupt a disulfide bridge essential for the proper folding of the

164 t suggest that the enzyme does not require a disulfide bridge for its activity as suggested elsewhere

165 on, partial oligomerization was observed via disulfide bridge formation at cysteine 482 in close prox

166 es that form a disulfide bridge in FGF23-WT; disulfide bridge formation in FGF23-WT is dispensable fo

167 stability of the PLN pentameric assembly via disulfide bridge formation, preventing its binding to Ca

168 such as at the skin surface, facilitates p4 disulfide bridge formation, required for the dimerizatio

169 itated synthesis, purification, and directed disulfide bridge formation.

170 Disulfide bridges formed at C349/C356 and C465/C468 of t

171 Disulfide bridges formed between C322 residues of differ

172 as a globular protein that dimerizes through disulfide bridges generated by cysteine oxidation.

173 disulfide connectivity in peptides with many disulfide bridges has proven to be laborious and general

174 characterized by exclusively intermolecular disulfide bridges have been analyzed by molecular modeli

175 Similarly, Cys-Cys disulfide bridges have been used to impose specific asso

176 This suggests a structural role of the disulfide bridges helping to mold the high-affinity doma

177 CTL4 and CTLMA2 are known to form a disulfide-bridged heterodimer via an N-terminal tri-cyst

178 d the modification to the peptide-containing disulfide-bridged hinge structure.

179 f the protein with reduced three out of four disulfide bridges), human insulin, bovine core histones,

180 In addition to the canonical internal disulfide bridge, Ig1 contains a second, solvent-exposed

181 the C-terminus that does not interfere with disulfide bridges, (ii) does not require activation, and

182 onomer in redox communication with an active disulfide bridge in a variant of the fold adopted by NTR

183 stigate the role of an intermittent internal disulfide bridge in determining NO oxidation kinetics at

184 R2 is flanked by two cysteines that form a disulfide bridge in FGF23-WT; disulfide bridge formation

185 The engineered disulfide bridge in m01s eliminated the self-association

186 Here, we investigate the role of the disulfide bridge in the dynamics of protein translocatio

187 Here we show that disruption of the disulfide bridge in the GES-5 carbapenemase by the C69G

188 solution has been activated by breaking the disulfide bridge in the triad Trp/Cys-Cys through absorp

189 ive, and as a consequence, disruption of the disulfide bridge in these enzymes destabilizes them, whi

190 ind that as much as 18% of all proteins with disulfide bridges in a non-redundant subset of PDB form

191 The results highlight the importance of disulfide bridges in a small bioactive peptide to bring

192 tion, resulting in formation of adventitious disulfide bridges in cell proteins.

193 Furthermore, we observed that disulfide bridges in eotaxin, epidermal growth factor, a

194 Surprisingly, two of the three disulfide bridges in IL-12alpha are dispensable for IL-1

195 tive cross-links was demonstrated by mapping disulfide bridges in RcsF, an outer membrane lipoprotein

196 tive disulfide bridge, which further reduces disulfide bridges in target proteins to regulate their s

197 We also identified two highly conserved disulfide bridges in the Cdc50 ectodomain.

198 that the successive reduction of one or both disulfide bridges in the dimer results in a stepwise dec

199 Four disulfide bridges in the extracellular domain, combined

200 teine residues generated by reduction of the disulfide bridges in the hinge region or surface lysine

201 s, constrained by the requirement of correct disulfide bridges in the hinge, resulted in the determin

202 e capacity of Cys-306 to form intermonomeric disulfide bridges in the presence of an oxidizing agent,

203 s at the C terminus and the fourth and fifth disulfide bridges, inhibited FXIIa with a Ki of 116 +/-

204 t quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into unfolded reduced proteins with th

205 dox reaction with copper ions resulting in a disulfide bridge involving Cys466.

206 ctural features of the protein, particularly disulfide bridges involving CXC motifs in the extracellu

207 nnels, which form oligomers held together by disulfide bridges involving Cys-73, accumulated in the p

208 Thus, either APP palmitoylation or disulfide bridges involving these Cys residues appear to

209 Here we show that the intramolecular disulfide bridge is constructed from the acyclic ene-dit

210 Our results demonstrate that the disulfide bridge is essential for stability but does not

211 The disulfide bridge is essential in maintaining the structu

212 tide linkage between LC and HC in place of a disulfide bridge is insufficient for productive LC trans

213 teine residues revealed that the Cys22-Cys28 disulfide bridge is not required for cleavage.

214 It was proposed that this conserved disulfide bridge is responsible for their carbapenemase

215 ogues of ProTx-II, in which one of the three disulfide bridges is replaced with a thioether linkage,

216 A defined arrangement of these disulfide bridges is unique to each isoform.

217 Cross-linking subunits through disulfide bridging is commonly used to stabilize multime

218 IIA (KIIIA), a 16-residue peptide with three disulfide bridges, is a pore blocker of voltage-gated so

219 The presence of the disulfide bridge isolates the active site from solvent a

220 d sheds light on the molecular processes and disulfide bridges isomerization underlying the conformat

221 examined leads to the formation of different disulfide-bridged isomers indicating the requirement of

222 These disulfide bridges link residue pairs > 40 A apart in the

223 ver medium- to long-range distances across a disulfide bridge linking loops L1 and L2, which constitu

224 how the possibility of previously unreported disulfide bridges linking the M1 and M3 transmembrane he

225 ino acids and the presence of three internal disulfide bridges may hamper its development for in vitr

226 rogate NS5A-NS5A interactions, implying that disulfide bridges may play a role in this interaction.

227 he C terminus, and two pairs of intrasubunit disulfide bridges may play an important role in its ther

228 The disulfide bridge must be on the trans compartment to ach

229 We show that the disulfide bridge must remain intact throughout LC transl

230 a 35-amino acid peptide cross-linked by two disulfide bridges named tau-AnmTX Ms 9a-1 (short name Ms

231 proposes (i) to reduce by ETD one of the two disulfide bridges of model peptides, resulting in the op

232 We show that the disulfide bridges of RTD-1, a member of the theta-defens

233 re, we observed 5 intra- and inter-subdomain disulfide bridges, of which 1 is unique in the C4 domain

234 ted potassium channel Kv2.1, which undergoes disulfide bridge oligomerization.

235 ereochemistry and the number of atoms in the disulfide bridge on binding affinity.

236 y thioredoxin-mediated redox modulation of a disulfide bridge on its gamma subunit.

237 own to be regulated by redox modulation of a disulfide bridge on the gamma-subunit through the ferred

238 AtSLP2 and is dependent on the formation of disulfide bridges on AtSLP2.

239 Here, we investigate the influence of disulfide bridges on protein dynamics through molecular

240 ohydrates for protein screening using either disulfide bridge or Schiff base imine immobilization che

241 Lassos arise in proteins with disulfide bridges (or in proteins with amide linkages),

242 Reduction of disulfide bridges partially restores activity.

243 s of other hydrophobic peptides with complex disulfide bridge patterns.

244 ticularly in the conservation of distinctive disulfide bridge patterns.

245 We have also investigated the folding and disulfide bridging patterns arising from different metho

246 It is a 17-amino-acid, two-disulfide bridge peptide.

247 We isolated and identified a disulfide-bridged peptide (+ICDINGVCVDA-), termed incept

248 facilitates the detailed characterization of disulfide-bridged peptides by mass spectrometry.

249 Human alpha-defensins are 3- to 5-kDa disulfide-bridged peptides with a multitude of antimicro

250 these two methodologies, by starting with a disulfide bridged phage display peptide which binds a hu

251 Disulfide bridges play a major role in defining the stru

252 hydrophobic loop to the G-actin surface by a disulfide bridge prevents filament formation.

253 uently results in incomplete dissociation of disulfide bridges prior to MS analysis, leading to a los

254 ctroscopy and the structure of an engineered disulfide-bridged Psu derivative reveal that the protein

255 lysis of cysteine mutants that disrupt these disulfide bridges revealed an inverse relationship betwe

256 oint mutants that engineer extra interdomain disulfide bridges rigidify the UGGT structure and exhibi

257 consistent with the replacement of up to two disulfide bridges (S-S) with a like number of trisulfide

258 he inhibitory helix onto the ETS domain by a disulfide bridge severely impairs, but does not abolish

259 ngal metabolite gliotoxin has a redox-active disulfide bridge spanning carbons 3 and 6 of a diketopip

260 The disulfide bridges stabilize the tertiary structure of th

261 nal SDS-PAGE, we detected CL-K1 and CL-L1 in disulfide bridge-stabilized complexes.

262 twork and by comparison to the corresponding disulfide-bridged structure.

263 tations at these sites and found they formed disulfide bridges that decreased the channel open dwell

264 Engineered disulfide bridges that locked the cassettes in two diffe

265 enom peptides from cone snails with multiple disulfide bridges that provide a rigid structural scaffo

266 ified the number of inter- or intramolecular disulfide bridges, the number of Cu(I) or Cu(II) ions, t

267 FI is composed of two chains linked by a disulfide bridge; the light chain comprises only the ser

268 Together with 2 nearby inter-subdomain disulfide bridges, this hinge induces slow conformationa

269 observed in minor abundance (with respect to disulfide bridged tiopronin species) before dramatically

270 onent of a low-density lipoprotein through a disulfide bridge to form lipoprotein(a).

271 ubunit encoded by an Abpa gene and linked by disulfide bridges to an ABPBG subunit encoded by an Abpb

272 paramyxovirus inhibitors, we have engineered disulfide bridges to introduce covalent links into the p

273 Nature uses interchain disulfide bridges to stabilize collagen trimers.

274 ompartments generally rely on intramolecular disulfide bridging to maintain conformation (e.g., album

275 sidues were exchanged with one another and a disulfide-bridged variant (H281C/S524C) where the two re

276 The disulfide-bridged variant showed approximately 20% incre

277 tionalized 1,4-bisthiophenol units linked by disulfide bridges was obtained by self-assembly on a gra

278 conditions, Ltn variants containing a second disulfide bridge were designed.

279 Phe-43) from the L3,4 loop and a distinctive disulfide bridge were shown to account for the high affi

280 Disulfide bridges were conserved and homogeneous, which

281 he analysis of a previous work in which four disulfide bridges were constructed in domain I of the Cr

282 nanoparticle stability, artificial covalent disulfide bridges were introduced throughout the VLP.

283 y of dendrimers displaying internally queued disulfide bridges were synthesized and exploited as flaw

284 However, pilins have disulfide bridges, whereas the major pseudopilins of T2S

285 ucing equivalents from FAD to a redox-active disulfide bridge, which further reduces disulfide bridge

286 The data indicate further that there is a disulfide bridge, which is not required for function, be

287 idge, Ig1 contains a second, solvent-exposed disulfide bridge, which our biochemical data indicate is

288 roportion displayed incorrect intermolecular disulfide bridging, which was hardly observed when C-man

289 The catalytic cysteine needs to form a disulfide bridge with a conserved cysteine, which is sub

290 ver it to the Cys residues of IscU, formed a disulfide bridge with Fdx in the presence of an oxidizin

291 ular domain of ENaC and likely involved in a disulfide bridge with the partner cysteine C394.

292 o cysteine (V51C) to make a symmetry-related disulfide bridge with the preexisting Cys 53 on the oppo

293 s may be unpaired and could therefore form a disulfide bridge with VCP.

294 aging through oxidation of proteins, forming disulfide bridges with cysteine or methionine sulfhydryl

295 otoxin contains eight cysteines forming four disulfide bridges with sequence similarities resembling

296 e 29 amino acid peptides cross-linked by two disulfide bridges, with a primary structure similar to o

297 method is demonstrated by partial mapping of disulfide bridges within a 37-kDa protein containing 16

298 PDI catalyzes an isomerization of disulfide bridges within the thioredoxin motif C600XXC60

299 can be attached to protein surfaces via two disulfide bridges, yielding a probe that is rigid relati

300 the introduction of appropriately positioned disulfide bridges yields a force resistant complex with