ライフサイエンスコーパス: dot blot

1 ncoprotein expression using western blot and dot blot.

2 Enhanced expression was verified by RNA dot blot.

3 s in mice of different ages via quantitative dot blot.

4 LL-37 expression was determined by immune dot blot.

5 and enables it to bind PtdIns(3,5)P(2) on a dot-blot.

6 cloned and subsequently confirmed by reverse dot blotting.

7 sica napus, using northern hybridisation and dot blotting.

8 esponse to AS, a hypothesis confirmed by DNA dot blotting.

9 and developed using the same protocol as in dot-blotting.

10 y from spiked wheat sample was comparable by dot-blot (78-91 %) and HPLC (65-87 %).

11 alization of PMCA products was performed via dot blots, a method that contributed to reduced processi

12 Whereas dot-blotting allows the use of very low quantities of sa

13 Western and protein dot blot analyses and indirect immunofluorescence were u

14 Dot blot analyses revealed no AT8 immunoreactivity above

15 Northern and dot blot analyses revealed that myosin XV is expressed i

16 Ligand and dot blot analyses revealed zinc-dependent binding of bio

17 The results of Northern and dot blot analyses show that ALF is expressed almost excl

18 Enzyme-linked immunosorbent assay and dot blot analyses showed MBP bound to the surface of the

19 Abs showed reactivity to CNF1 by Western and dot blot analyses.

20 eptor protein was detected using western and dot blot analyses.

21 t of these clones bound mAb TL4 in ELISA and dot blot analyses.

22 Northern and dot-blot analyses showed a large reduction in LOX-2 mRNA

23 at was further supported by Western-blot and dot-blot analyses, as well as by inhibition of binding b

24 Utilizing Northern dot blot analysis and a quantitative reverse transcripti

25 Northern dot blot analysis and comparative densitometry confirmed

26 in the transfected cells was demonstrated by dot blot analysis and ELISA.

27 n of these genes by 2,4-DNP was confirmed by dot blot analysis and two of them were confirmed to be i

28 Dot blot analysis demonstrated that nonfibrillar Abeta o

29 Northern dot blot analysis demonstrated that Tctex1/Tctex2 family

30 Dot blot analysis established that packaging efficiency

31 Immuno-dot blot analysis identified the cis-syn cyclobutane pyr

32 cids, respectively, were measured by Western dot blot analysis immediately after exposure to light.

33 Food allergens were measured by dot blot analysis in mattress dust from 143 homes in Osl

34 Southern and dot blot analysis indicate that the tigA gene is present

35 RNA dot blot analysis of 100 cases of primary tumors suggest

36 DNA dot blot analysis of 106 V. cholerae strains showed the

37 entrations of glandular protein when Western dot blot analysis of collagens I and III and laminin, re

38 atoid papulosis cases were then subjected to dot blot analysis of genomic DNA using a full-length HTL

39 RNA dot blot analysis of human multiple tissue expression ar

40 ss different cancer cell lines take 1 d, the dot blot analysis of mouse liver tissues takes 1 d and t

41 Dot blot analysis of mRNA from 50 human tissues indicate

42 Data from dot blot analysis of PCR products were consistent with t

43 Dot blot analysis of RNA preparations confirmed the low-

44 According to fluorescence polarization and dot blot analysis of synthetic DnaK fragments and labele

45 Western dot blot analysis showed a 3.4-fold increase in protein

46 Dot blot analysis showed that the "raft" associated gang

47 Dot blot analysis using a panel of synthesized oligosacc

48 Dot blot analysis with 11 DNA sequences from this PAI de

49 d difference in intensity were rescreened by dot blot analysis with the same probes and with mixed cD

50 By dot blot analysis, 4-HNE- and 4-HHE-modified proteins we

51 Using bacterial lysates in dot blot analysis, a panel of sera from normal individua

52 ogical, fluorescence-activated cell sorting, dot blot analysis, and in vitro/ex vivo studies were use

53 s, we employed immunofluorescence labelling, dot blot analysis, Fourier transform Raman spectroscopy,

54 uIC products were conducted by western blot, dot blot analysis, Raman spectroscopy, atomic force micr

55 AbetaO(42) in the target samples against the dot blot analysis, which requires more than 14 h to prov

56 after IL-8 exposure, was observed using RNA dot blot analysis.

57 he prototype subtype P1.14 strain, S3446, by dot blot analysis.

58 growing bacteria was investigated by immuno-dot blot analysis.

59 measure the relative telomere DNA content by dot blot analysis.

60 extrinsic fluorescence, UV, FTIR, DSC, SEC, dot-blot analysis and in vitro digestibility.

61 Dot-blot analysis and RT-PCR revealed that human PKD1L1

62 Human RNA dot-blot analysis detected this mRNA in all tissues exam

63 Through dot-blot analysis employing m5C antibodies against chrom

64 Dot-blot analysis of p38delta mRNA in 50 human tissues r

65 Dot-blot analysis of the isolated clones verified differ

66 t had accumulated in cultured cells, whereas dot-blot analysis revealed binding to both A2E and A2E-r

67 In addition, dot-blot analysis showed approximately 5-10% of C5 and C

68 its direct association to the enzyme because dot-blot analysis using antibody to glutathione S-transf

69 m WHV DNA was not detectable by conventional dot-blot analysis, hepatic WHV-DNA replicative intermedi

70 By dot-blot analysis, we estimate there are 150,000, 4,000,

71 Using library screening and dot-blot analysis, we estimate there are 25,000 copies o

72 Modified retinal proteins were quantified by dot-blot analysis.

73 trol extracellular vesicles as determined by dot blot and atomic force microscopy.

74 DNA dot blot and biochemical assay of viral DNA polymerase a

75 Stromelysin mRNA levels were evaluated by dot blot and by reverse transcription, followed by polym

76 Dot blot and ELISA assays demonstrate the inhibition of

77 eningitidis were examined for MBL binding by dot blot and ELISA.

78 ing to An. gambiae BBMV and bound Cry11Ba on dot blot and microtiter plate binding assays with a calc

79 Dot blot and Northern analyses of MCPS MAbs revealed tha

80 ir expression patterns were confirmed by RNA dot blot and RT-PCR analyses.

81 transcription was confirmed by quantitative dot blot and Western blot analyses.

82 Dot blot and western blot assays are used to assess anti

83 IBM autoantigen, which was then confirmed in dot blot and Western blot assays using recombinant cN1A

84 ibody were determined in infected monkeys by dot blot and Western blot assays, respectively.

85 and plasmin protein levels were measured by dot blot and Western blot, respectively, while plasmin a

86 Viremia, measured by dot blot and/or PCR, and SPV-specific antibodies were de

87 Selected cosmids were spotted on dot blots and assigned to bins defined by YACs.

88 CMV-specific DNA dot blots and biochemical enzyme assays indicated that,

89 ometric analysis of semiquantitative Western dot blots and by immunohistochemistry, using 4-HNE- and

90 Both dot blots and immunocytochemistry show that allurin is s

91 expressed AgALP1(t) bound [(125)I]Cry11Ba on dot blots and reduced the level of binding of [(125)I]Cr

92 protein assays including mass spectrometry, dot blots and Western blotting were developed to determi

93 DU-145 cells was demonstrated by Western and dot blotting and flow cytometry with monoclonal antibodi

94 actin, or NC1 domain of type VII collagen by dot blotting and western blotting.

95 raised to the PorB peptides and were used in dot-blot and ELISA-based absorption studies with viable

96 Using dot-blot and primer extension assays, we measured the su

97 asured catalase protein expression by immune dot-blotting and catalase mRNA by a real-time polymerase

98 Two other assays, dot-blotting and immunoblotting, detect KS in complex mi

99 pimonidazole-protein adduct quantification (dot blot) and as a surrogate for transcriptional activat

100 aLP bound [(125)I]Cry1A toxins under native (dot blot) and denaturing (ligand blot) conditions.

101 inst major allergens by western blot, ELISA, dot blot, and cellular assays.

102 ymerase chain reaction (PCR)/ Southern blot, dot blot, and Southern blot analyses.

103 gomers (measured by single-molecule imaging, dot blots, and Bio-Layer Interferometry) inhibited AB ol

104 easured by transmission electron microscopy, dot blots, and size-exclusion chromatography).

105 sed transcriptional reporter constructs, RNA dot blots, and Western blots to examine the expression o

106 irst shown using fluorescence microscopy and dot blotting, and further demonstrated using flow cytome

107 Northern blot, dot-blot, and reverse transcription-coupled PCR analyses

108 have been developed and validated by ELISA, dot-blot, and Western blot analysis.

109 biotinylated substrate in conjunction with a dot blot apparatus to eliminate the use of radioactive s

110 Peptides are assayed in a 96-well dot blot apparatus using membranes that enable partition

111 ification method that combines a solid-state dot blot approach with radiolabel detection via liquid s

112 We describe use of a convenient "dot-blot" approach to screen 10 different PH domains for

113 y breast tumors, we screened a breast cancer dot blot array of normalized cDNA from 50 breast tumors

114 Western blotting (Dot Blot) as an immunological method confirmed biologica

115 the constructs were tested in a solid-phase dot blot assay followed by confirmatory quantitative che

116 The sensitivity and specificity of a dot blot assay for detection of human antibodies with th

117 ave developed an amplification-based reverse dot blot assay for the detection of specific sites of in

118 An immuno-dot blot assay that could detect LP at levels as low as

119 The detection of hTSH in a dot blot assay with radiolabeled T-15 RNA was demonstrat

120 se 34 IFA-positive sera were positive by the dot blot assay with rP30, distinguishing them from IFA-n

121 IL15-induced recombinant tau oligomers and a dot blot assay, we discovered a mAb (M204) that binds ol

122 xigenin (DIG)-labeled human lactoferrin in a dot blot assay.

123 er typed for colonization factor antigens by dot blot assay.

124 12 colonization factor antigens (CFAs) by a dot blot assay.

125 qualitatively ranked by a new Dynabead-based dot blot assay.

126 which is specific for sialylated species, by dot blot assay; this reactivity was also reversed by neu

127 a visual detection agent in rapid, sensitive dot-blot assay (LOD 0.39 mug/kg).

128 Here we describe an efficient dot-blot assay for high-throughput screening of two enzy

129 Finally, we developed a dot-blot assay for the glycan-specific IgG antibody that

130 Therefore, the developed dot-blot assay holds promise for monitoring AFB1 contami

131 Dot-blot assay reveals that the TrioN-PH domain does not

132 njugated to gold nanoparticles and used in a dot-blot assay to detect autoantibodies in serum samples

133 We modified the traditional dot-blot assay to serve as an identity test for monoclon

134 Using the MAb dot-blot assay, 5 of 16 commercial DNA products obtained

135 as quantified by periodic acid-Schiff's base dot-blot assay, using purified pig gastric mucin as a st

136 omatography and a recently developed polymer dot-blot assay.

137 before ISH using a RNA probe quantification (dot blot) assay.

138 RNA dot blot assays confirmed that expression of the acidic

139 ction by EIS was further supported by immuno dot blot assays for oligomeric and fibrillar components.

140 report we used yeast two-hybrid and in vitro dot blot assays to further examine the requirements for

141 he rDer f 21 allergen and carried out immuno-dot blot assays using 24 atopic sera.

142 n immunoblotting, the results of Western and dot blot assays with rP30 matched 100% with the IFA test

143 ion sequencing technology), and quantitative dot blot assays.

144 luble heparin specifically in inhibition and dot blot assays.

145 Collectively, the EB absorption and dot-blot assays established that the immunoreactive PorB

146 However, the majority of dot-blot assays have only limited sensitivity and are on

147 ant corroborated the results of quantitative dot-blot assays of ETA.

148 em, monoclonal antibodies (MAbs), ELISA, and dot-blot assays were developed for the specific identifi

149 nditions, as assayed by quantitative Western dot-blot assays.

150 A novel dot blot-based assay system for the detection of IgE aga

151 rated in the Namalwa B lymphoma cell line by dot blot binding assays as well as confocal microscopy.

152 emonstrate a simple and robust drug-assisted dot blot bioassay for endotoxin detection that can be us

153 A dot blot capsular type was assigned to 99% (303 of 306)

154 colonizing isolates were used to compare our dot blot capsular typing (DBCT) identification method wi

155 .2 nm for TEM images), and most importantly, dot blotting confirmed immunological activity of the col

156 A cDNA dot blot consisting of differentially expressed genes re

157 We demonstrated that the new dot blot coupled to biotin-Si-NPs successfully detected

158 CR/Southern blotting, Northern blotting, and dot blotting demonstrated the presence of the platelet-t

159 The new dot blot detection system resolved individual IgE sensit

160 matography combined with rapid and sensitive dot-blot detection.

161 indings suggest that the developed MAb based dot blot ELISA is a simple, rapid performed in less than

162 ere included for the evaluation of MAb-based dot blot ELISA.

163 ophysical assays including amyloid kinetics, dot blot, ELISA, and TEM show that 5 effectively inhibit

164 In addition, the potential of UCNP-based dot-blot for real sample analysis was confirmed by analy

165 od relative to that of a previously reported dot blot format.

166 enous beading, whereas those with 4-quadrant dot-blot hemorrhages (4Q DBH) had 3.84 higher HR of deve

167 Dot/blot hemorrhages, cotton-wool spots, and small nonpe

168 nce of B. pertussis PCR products detected by dot blot hybridization alone.

169 thods to determine the PlA genotype: reverse dot blot hybridization and allele-specific restriction d

170 representative isolates was also analyzed by dot blot hybridization and amplification with the polyme

171 HPV was detected by dot blot hybridization and genotyped by the liquid bead

172 Dot blot hybridization and PCR amplification of 14 Ara(+

173 ch 11 cDNA clones were found by differential dot blot hybridization and virtual Northern analysis to

174 nd FAP59/64 primer systems and identified by dot blot hybridization and/or direct sequencing.

175 In addition, dot blot hybridization determined that 3 of 35 strains t

176 investigated by in situ hybridization and by dot blot hybridization for opticin.

177 n reaction (PCR), real time PCR (RT-PCR) and dot blot hybridization have also been proposed for patho

178 Parvovirus B19 (B19) DNA was detected by dot blot hybridization in sera from 5 (17%) of 30 human

179 However, genotype determination by dot blot hybridization is laborious and requires at leas

180 Dot blot hybridization of DNA samples from the F1 offspr

181 Dot blot hybridization revealed that the C. jejuni genes

182 Dot blot hybridization showed that the three probes disp

183 her agarose gel electrophoresis (PCR-gel) or dot blot hybridization with (32)P-labeled oligonucleotid

184 We describe a typing method that uses DNA dot blot hybridization with probes generated by PCR from

185 by the subtraction were screened, using DNA dot blot hybridization, against a collection of 88 uropa

186 ganisms were also distinguishable by DNA-DNA dot blot hybridization, by sequences of two hypervariabl

187 he IS481 PCR, with either electrophoresis or dot blot hybridization, is a sensitive assay; however, a

188 Using PCR and dot blot hybridization, we determined that the his opero

189 c lesions was enhanced by a chemiluminescent dot blot hybridization, which produced a sensitivity of

190 rofile of putative adhesin-encoding genes by dot blot hybridization.

191 1 L1 consensus primer PCR method followed by dot blot hybridization.

192 s media and throat strains was determined by dot blot hybridization.

193 by MY09/MY11 L1 primer PCR and type-specific dot blot hybridization.

194 Thirty specimens were positive by PCR with dot blot hybridization; no negative control specimens sh

195 er RNA (mRNA) were evaluated by Northern and dot-blot hybridization analyses.

196 by dynamic chemistry (ChemNAT) with reverse dot-blot hybridization in an array format for a rapid an

197 ium salinarum GRB chromosome was analyzed by dot-blot hybridization of an ordered cosmid library usin

198 Traditional 'dot-blot hybridization' or PCR-based assays for identify

199 e amplification by Southern hybridization or dot-blot hybridization, and for gene expression by North

200 cts were detected by gel electrophoresis and dot-blot hybridization.

201 tative virulence genes using high-throughput dot-blot hybridization.

202 -myc, c-erb, and c-src was quantitated by a "dot-blot" hybridization assay.

203 On the basis of dot-blot hybridizations, seven repetitive DNA motifs acc

204 similar with purified mRNA and total RNA in dot blot hydridizations for cultures grown with or witho

205 wer detection limit and analysis time than a dot blot immunoassay (8.88x10(6) cfu mL(-1) for LOD and

206 or BKV, none of the samples were found by a dot blot immunoassay to have antibodies which cross-reac

207 evaluated by Western immunoblot analysis and dot blot immunoassay.

208 and the fractions collected were screened by dot-blot immunoassay by means of monoclonal antibodies g

209 First, the dot-blot immunoassay on a nitrocellulose membrane was op

210 zation-blocking activity were carried out by dot-blot immunoassays and showed that neuroprotective ex

211 Dot-blot immunoassays are widely used for the user-frien

212 sitive labels that enable the development of dot-blot immunoassays for quantitative analysis of low-a

213 Immunoassays such as dot blot, immunoblot, and enzyme-linked immunosorbent as

214 s towards monomeric and polymeric SUMO1-4 in dot-blots, immunoblots, immunofluorescence and immunopre

215 Studies by using dot blotting, immunoblotting, circular dichroism spectro

216 s on EV membrane surfaces, which we call "EV dot blotting", inspired by conventional dot blotting tec

217 Microwave-assisted dot blotting is suggested as an effective way of facilit

218 Dot-blot is a versatile and simple analysis to perform.

219 es and an epidemic genetic background by DNA dot blotting, IS1004 fingerprinting, and restriction fra

220 assay, Western blotting, capture assays, and dot blots, less than 25% are capable of neutralizing let

221 nd ImageQuant software demonstrated that the dot blot method can be used to rapidly analyze a large n

222 We developed an immunochemical dot blot method for quantitation of protein carbonylatio

223 eminal ganglia of rabbits to demonstrate the dot blot method of PCR product analysis.

224 The dot blotting method further verifies a positive reaction

225 re determined using a semiautomated, reverse dot-blot method.

226 by means of a noninvasive PCR-based reverse-dot-blot method.

227 by 3-5 orders of magnitude from conventional Dot-blot methods.

228 We studied, by dot-blot, NACP levels in the frontal cortex of AD cases

229 screening and further confirmed in vitro by dot blots, native electrophoresis, and fluorescence stud

230 ucrose density centrifugation and subsequent dot-blot Northern analysis revealed that antioxidants re

231 outine surveillance samples were analyzed by dot-blot Northern hybridization to detect RotaTeq vaccin

232 the length of the PAI and were hybridized to dot blots of genomic DNA isolated from clinical isolates

233 Moreover, immunostainings and dot blots of optic nerve and myelin showed that expressi

234 VL-horseradish peroxidase conjugate bound to dot blots of VSP or SVL, and binding was inhibited by po

235 Screening of an RNA dot-blot panel confirmed that Kell is primarily expresse

236 Here, we introduce a novel dot-blot particle-linked immunosorbent assay (PLISA) wit

237 Hence, such an enhanced dot blot paves the path to the development of a portable

238 roliters of undiluted CSF, sample digestion, dot blotting, Perls' histochemistry, 3,3'-diaminobenzidi

239 presence of ETEC DNA of positive samples by dot blot procedure.

240 The feasibility of Quantitative Dot Blot (QDB) method for this purpose was explored in t

241 We developed a reverse dot blot (RDB) to screen for multiple serotypes in these

242 Densitometric analysis of dot blot reactions showed a positive correlation between

243 d using pools of BAC DNA in combination with dot-blots reveals the locus specificity of individual BA

244 ), rapid amplification of cDNA ends PCR, RNA dot blotting, RNase protection assay, and Northern blot

245 In dot-blotting, samples were directly blotted onto nitroce

246 TLV-I in any PCR-positive case using genomic dot blotting (sensitivity > 10(-2)), and (iii) negative

247 A human tissue RNA dot blot showed that RGS5 message is highest in aorta, f

248 Northern and dot blotting showed that SAMP14 expression was testis-sp

249 A)-specific peptide were characterized using dot blot, sodium dodecyl sulphate-polyacrylamide gel ele

250 Dot blot studies further validated the BAX-IGFBP-3 bindi

251 "EV dot blotting", inspired by conventional dot blotting techniques.

252 ay (RPA) assays use micro-scale, cell lysate dot blots that are printed to a substrate, followed by q

253 With dot blots, the time savings was accompanied by a decreas

254 immunoprecipitation, immunofluorescence, and dot blots to examine the effects of TNF-alpha.

255 cted candidate EST clones were tested by RNA dot blots to examine tissue specificity and by Northern

256 Evaluation of dot-blot using certified reference material and 146 food

257 lfate-polyacrylamide gel electrophoresis and dot blot, using both monoclonal anti-human Epo antibody

258 ral monolayer compartments were evaluated by dot-blot, using the sera of milk allergic children (N=5)

259 Dot blotting was also used to analyze expression changes

260 To improve the response of standard dot blots, we have applied a new enhancement strategy th

261 Using Northern blots and RNA dot blots, we have now found that XAGE-1 is highly expre

262 Using yeast two-hybrid analysis and in vitro dot-blots, we show that MLK2 and MLK3 interact with the

263 Dot blots were used as a quantitative assessment of cort

264 A2 (PLA2) activity, as determined by native dot blot Western and PLA2 assays, respectively.

265 halis heterologous isolates were screened by dot blot with a panel of four additional MAbs specific f

266 with results from a previous analysis using dot blots with a radiolabeled nested generic probe mix a

267 n (RT-PCR), colony hybridization and reverse dot blot yielded three distinct probes.