ライフサイエンスコーパス: double-labeled

1 which retrogradely labeled SON neurons were double labeled.

2 rage of 98% of descending brain neurons were double labeled.

3 including many unidentified RS neurons, were double labeled.

4 owever fewer than 3% of labeled neurons were double labeled.

5 n VLM and NTS, 74% and 42% respectively were double labeled.

6 Significantly, very few neurons (<2%) were double-labeled.

7 n is deuterated and the C-terminal domain is double-labeled.

8 were fed single-labeled [14C]AGE-ovalbumin, double-labeled [14C-125I]AGE-ovalbumin, or control 125I-

9 Double-labeled 25 microns tissue sections were examined

10 Double-labeled 5-HT3A/CB1 neurons were found in the ante

11 in conjunction with GAD immunohistochemistry double labeled a high percentage of neurons in both the

12 on the synthesis and use of an isotopically double-labeled Abeta1-15 glycopeptide, carrying the core

13 A-cal is used to measure binding events, and double-labeled AEDANS,DDP-T34C/T110/C-calmodulin (DA-cal

14 Comparable proportions of cells were double-labeled after sucrose or quinine, consistent with

15 e Fos-like immunoreactive (FLI) neurons were double-labeled; after RF injections the proportion was 9

16 Cultures were double-labeled against GABA and the neuronal marker MAP2

17 oat protein gene of CTV (CTV-p25) and detect double labeled amplicons on a sandwich immunoassay by de

18 assay was performed with a primary antibody, double-labeled amplicons, and fluorophore-labeled strept

19 mparison of SP and SPR immunoreactivities in double-labeled and adjacent single-labeled sections reve

20 Hyperpolarized, double-labeled aspirin was well tolerated in mice and co

21 Some double-labeled BDA/VGAT varicosities were seen apposed t

22 content and the numbers of insulin and BrdU double-labeled beta cells in the islets.

23 To prove this hypothesis, we tracked double-labeled BMSCs in implantation sites created in nu

24 Some BrdU-labeled cells were also double labeled by antibodies to glial-specific (antikera

25 From adjacent sections double labeled by fluorescent immunohistochemical stains

26 When double labeled by in situ hybridization, these neurons c

27 These recoverin-positive cells were not double-labeled by cell-specific markers expressed by pho

28 ptide induced 75% of plasmatocytes that were double-labeled by the monoclonal antibodies 49G3A3 and 4

29 Efferent neurons were double-labeled by the unilateral injections of separate

30 esonance energy transfer experiments using a double-labeled CaM molecule were performed and indicated

31 For L4 and L5, respectively, estimates of double-labeled CaMKII alpha neurons showed 34% +/- 2% an

32 istochemistry, and percentages of single and double-labeled CaMKIIalpha neurons were determined.

33 Double-labeled cancer cells were imaged at the cellular

34 treated animals by the increasing number of double labeled CD68+/CD206+ cells in the cranial and cen

35 d the dorsal and linear raphe nuclei, but no double labeled cells were seen in the amygdaloid nuclei

36 nt topographically organized zones, with few double-labeled cells (~4-6%).

37 neurons, with the percentage of LHRH+/c-Fos+ double-labeled cells approximately fivefold higher on th

38 ER-beta/PV double-labeled cells are also observed within the latera

39 Double-labeled cells are injected by various methods.

40 The location of these double-labeled cells around the locus coeruleus correspo

41 The percentage of double-labeled cells as a proportion of either LA-projec

42 The double-labeled cells constituted fewer than 2% of the ce

43 These findings suggest that double-labeled cells contribute substantially to many of

44 t commonly observed in the PIN (43.7% of all double-labeled cells following injections into LA and th

45 Double-labeled cells for ERalpha and calbindin-D(28k) we

46 The double-labeled cells form a small percentage of the cort

47 The topographic distribution of double-labeled cells in the thalamic nuclei resembled th

48 Double-labeled cells providing inputs to both rectus and

49 ER-beta/PV double-labeled cells represent 23.3% +/- 1.6% (intact) a

50 Double-labeled cells were concentrated at the border bet

51 ns revealed that significantly more (18-33%) double-labeled cells were detected in the horizontal lim

52 hat, for all three primary sensory cortices, double-labeled cells were extremely rare, indicating tha

53 Double-labeled cells were found in all the thalamic regi

54 FG/c-Fos double-labeled cells were found in forebrain regions inc

55 Substantial numbers of FG/cFos double-labeled cells were found in forebrain regions, in

56 Substantial FG/cFos double-labeled cells were found in forebrain regions, in

57 -b injections were centered in the PVH, many double-labeled cells were found within the caudal DMH.

58 ge marker OX-42 on postnatal day 10 in vivo, double-labeled cells were identified in the cerebral cor

59 Generally, double-labeled cells were in expected high-frequency ton

60 Most other double-labeled cells were located in peribrachial region

61 Half of the double-labeled cells were located in the locus coeruleus

62 Although double-labeled cells were never observed, afferents to t

63 Relatively few double-labeled cells were observed in both the A4 and th

64 abeled cells in LH co-expressed SS, while no double-labeled cells were observed in IC.

65 entered in the subparaventricular zone, many double-labeled cells were observed in the dorsomedial su

66 Numerous double-labeled cells were observed throughout the same a

67 Double-labeled cells were present in almost all perioliv

68 Elsewhere, double-labeled cells were very scarce, making up approxi

69 cells, there is a considerable population of double-labeled cells with approximately 30% of each popu

70 Of the double-labeled cells, 41% also were immunoreactive for 5

71 sterior VTA were both effective in producing double-labeled cells, and an anterior-posterior topograp

72 The inferior colliculi contained very few double-labeled cells, indicating that the projections to

73 OA and MePD contained the greatest number of double-labeled cells.

74 thymidine 24 hr after the BrdU and seeing no double-labeled cells.

75 Rb mRNA was found; thus, there were very few double-labeled cells.

76 ess can be observed to emanate from BrdU/DCX double-labeled cells.

77 Following ovx, the percentage of double labeled cholinergic basal forebrain neurons also

78 ppearance and to investigate the presence of double-labeled cones in sections and wholemounts of huma

79 g to bones through the use of differentially double-labeled derivatives.

80 Conventional molecular beacons require double-labeled DNA sequences, which are costly and time-

81 lowing the principle of proportionality, and double-labeled duplex DNA was synthesized.

82 Hz 19F NMR spectrum of the microcrystalline, double-labeled enzyme complex has five resolved lines un

83 The double-labeled enzyme retained essentially full catalyti

84 y 15, we assessed PKCdelta and SOM, Fos, and double-labeled expression in CeL and central amygdala me

85 then determining the relative proportions of double-labeled FF and FB neurons in an area intermediate

86 However, the density of these double-labeled fiber segments varied considerably depend

87 Double-labeled fiber segments were also present, but spa

88 Double-labeled fiber segments, typically of fine caliber

89 onfocal analysis demonstrated that NPY/PHA-L double-labeled fibers came in close apposition to CRH pe

90 In the median eminence, NPY/PHA-L double-labeled fibers were found both in the inner and t

91 the lateral septum (LS), whereas only a few double-labeled fibers were found in other brain areas in

92 However, very few double-labeled fibers were found in the proximity of CRH

93 In PVH, NPY and PHA-L double-labeled fibers were found mainly in the parvocell

94 Scattered double-labeled fibers were present in the lateral septal

95 Thus, double-labeled fibers were unequivocally identified as s

96 Third, using the technique of double-labeled fluorescent immunocytochemistry, the rela

97 A double-labeled fluorescent probe was designed and evalua

98 Double-labeled fluorescent staining was used to examine

99 tion of distance from the center of plaques (double labeled for A beta peptide and microglia) reveale

100 rase (ChAT) in the geniculate A-laminae were double labeled for BNOS.

101 s, but not other CRF-containing nuclei, were double labeled for CRF and PRV.

102 he BSTL as a retrograde tracer, neurons were double labeled for CTB and PACAP or VIP immunohistochemi

103 Cells double labeled for GABA and MOR1 were common in the peri

104 In rats, cells double labeled for GABA and MOR1 were observed in layers

105 They were also double labeled for GFP and RPE65 or MITF.

106 The majority of these syncytia could be double labeled for HIV-1 RNA and a dendritic cell marker

107 Tissue sections were also double labeled for Melan-A and CD34.

108 These findings were confirmed in sections double labeled for NGF and CGRP immunoreactivity.

109 trols and 1 year post-injection retinas were double labeled for protein kinase C and tyrosine hydroxy

110 elected cases, series of brain sections were double labeled for PRV-Ba and tyrosine hydroxylase to de

111 mall percentage of F4-80+ microglia are also double labeled for SHP-1 at early times post-MCAO.

112 maniculatus, and Peromyscus polionotus, and double labeled for the expression of ERalpha and AVP imm

113 number of cells in the SFO, OV and MePO were double-labeled for both FOS-ir and fluoro-gold.

114 riatopallidal and striatonigral neurons were double-labeled for both mGluR1a or mGluR5.

115 after a 3-wk survival time, there were cells double-labeled for BrdUrd and either TuJ1 or neuronal nu

116 and neocortex; some of the latter cells were double-labeled for BrdUrd and TuJ1.

117 AII amacrine cells also were double-labeled for calretinin and parvalbumin; however,

118 The proportions of TH cell profiles double-labeled for ChAT or VIP significantly increased b

119 In sections double-labeled for CR and mGluR1alpha, the patterns of d

120 -estradiol, the number of perirhinal neurons double-labeled for ER-beta/GABA was reduced by 28% (P<0.

121 ctory organs were cryosectioned (10 microm), double-labeled for Galpha(olf), Galpha(q), or PLC140, an

122 cetyltransferase-immunoreactive neurons were double-labeled for mGluR1a-like immunoreactivity.

123 ion was confirmed by the presence of neurons double-labeled for NADPH-d and Fluoro-Gold.

124 than a third of the cholinergic neurons were double-labeled for NOS.

125 bunits, 56% of parvalbumin interneurons were double-labeled for NR2A/2B, and all identified cholinerg

126 ndin-containing striatal matrix neurons were double-labeled for NR2A/2B.

127 ) of mGluR1alpha-immunopositive neurons were double-labeled for somatostatin.

128 sections from developing rat cerebellum were double-labeled for TAG-1 and the Purkinje cell-specific

129 cells in the inner nuclear layer (INL) were double-labeled for TH immunoreactivity.

130 The majority of FG-filled cells double-labeled for TH were found in the dorsocaudal A10

131 Hippocampal sections were double-labeled for the alpha1, alpha4, gamma2, and delta

132 imulus-induced neuronal activation, and were double-labeled for tyrosine hydroxylase to identify cate

133 In response to hemorrhage, double-labeled Fos/5-HT neurons were located in the B3 r

134 samples that are generally different from a double-labeled FRET sample, or by the use of sophisticat

135 cumented using the characteristic pattern of double-labeled gene trees.

136 R in the VTA and all subdivisions of the SN; double-labeled GFRalpha-1/CR neurons were distributed in

137 ive glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly

138 Targets exposed to double-labeled granzyme B-serglycin complexes show solel

139 Double-labeled (i.e., CTb(+), Fos(+)) neurons were found

140 Using double-labeled immune-fluorescence confocal microscopy,

141 Double-labeled immunocytochemistry was performed to iden

142 We carried out single- and double-labeled immunohistochemical and pathway tracing s

143 These nerves were double-labeled immunohistochemically with an anti-idioty

144 rmal (n=2) rats were processed by single and double labeled immunohistochemistry for cellular identif

145 Double-labeled immunohistochemistry demonstrated co-loca

146 ion, Northern and Western blot analyses, and double-labeled immunohistochemistry; (3) determined casp

147 Double-labeled immunostaining for MCP-1 and neuron speci

148 scriptional activity were investigated using double-labeled in situ hybridization, Northern blot and

149 A subpopulation of these neurons (double labeled) in the interstitial and intermediate sub

150 Less than 1% of cells were double-labeled, indicating that the populations of cells

151 Mouse LGs were processed for single- and double-labeled indirect immunofluorescence studies and e

152 crimal glands were processed for single- and double-labeled indirect immunofluorescence studies.

153 Double-labeled interneurons were mainly located in the d

154 We double labeled Ipc axons and their presumptive postsynap

155 now applied single-molecule FRET to Cy3, Cy5 double-labeled LacI-DNA loops diffusing freely in soluti

156 econd label (5BrdU) was distributed from the double-labeled LREC to unlabeled mammary cells while 3HT

157 Confocal analysis of double-labeled material indicated that AII dendrites spi

158 ne bipolar cells were immunolabeled, and the double-labeled material was analyzed.

159 of fine processes called telodendria and, in double-labeled material, Cx36 plaques were located preci

160 study femurs were isolated from genetically double-labeled mBSP9.0Luc/beta-ACT-EGFP transgenic mice

161 f these compounds was readily converted to a double-labeled mixed-chain phosphatidylcholine applicabl

162 abeled motoneurons and higher proportions of double-labeled motoneurons than untransected rats.

163 lexa 488 (donor) and Alexa 546 (acceptor) in double-labeled MT allows the monitoring of metal binding

164 The distance distributions between three double-labeled mutants, in the collapsed transient state

165 co-expressed enkephalin (Enk), and Ucn 3/Enk double-labeled nerve fibers and terminals were observed

166 erulear dendritic region, with an occasional double-labeled neuron in the LC.

167 GFR alpha-1/PV double labeled neurons were also detected in the s. orie

168 Red, green and double labeled neurons were found bilaterally in the PMC

169 othalamic nucleus showed only a few Nurr1/TH double labeled neurons, while TH-immunoreactive neurons

170 ope, and scored for the number of single and double labeled neurons.

171 ed with no laminar segregation, we found few double-labeled neurons ( approximately 5% of each singly

172 However, double-labeled neurons also exhibited a preferential dis

173 ded amygdala-like afferents but produced few double-labeled neurons and these only when paired with v

174 fferent tracers were injected in SII and MI, double-labeled neurons appeared above and below the laye

175 Similarly, the density of c-fos-NR1 double-labeled neurons during hypoxia progressively incr

176 tral PBN resulted in a greater percentage of double-labeled neurons in BNST and CeA compared to cauda

177 d in significant increases in the numbers of double-labeled neurons in both the NST and RF, suggestin

178 These differential distributions of double-labeled neurons in the NST and RF suggest a role

179 Double-labeled neurons in the NTS were located primarily

180 In contrast, double-labeled neurons in the PAG were almost never retr

181 although there were a significant number of double-labeled neurons in the RF, the major concentratio

182 sion as well as the number of FluoroGold/Fos double-labeled neurons in the ventral Vi/Vc (P<0.05).

183 d neurons as well as alpha4/TH and alpha7/TH double-labeled neurons increased in the A1, A2 and A6 br

184 The number of single- and double-labeled neurons located in the right (ipsilateral

185 ei revealed a small, but constant, number of double-labeled neurons located predominantly ipsilateral

186 The distribution of double-labeled neurons mirrored that of the projection n

187 Minor PACAP projections with scattered double-labeled neurons originated from the parabrachial

188 A third, sparser group of double-labeled neurons projected to both areas; we terme

189 ime-lapse confocal microscopy of individual, double-labeled neurons revealed a coincident, activity-d

190 We found double-labeled neurons surrounding the median eminence a

191 sker representations of SII and MI to detect double-labeled neurons that would indicate that some SI

192 However, the proportion of double-labeled neurons was consistently approximately 1%

193 of cortical GABA, ER-beta, and ER-beta/GABA double-labeled neurons was examined.

194 For SS, the percentage of double-labeled neurons was more forebrain site specific

195 However, these double-labeled neurons were a modest percentage of the s

196 Sparse populations of double-labeled neurons were found in both V1 and V2 but

197 No double-labeled neurons were found in the dorsal Vi/Vc an

198 A very small number of double-labeled neurons were found in the pontine micturi

199 al sphincter neurons and bladder neurons and double-labeled neurons were found in the reticular regio

200 Double-labeled neurons were found in the reticular, raph

201 ause it normally stains granule cells), many double-labeled neurons were located at the hilar/CA3 bor

202 Overall, double-labeled neurons were most numerous in the caudal

203 In the NST, double-labeled neurons were most numerous in the rostral

204 Moderate numbers of double-labeled neurons were observed following combined

205 Double-labeled neurons were observed in all noradrenergi

206 Single- and double-labeled neurons were observed in the A2 and A1 NE

207 r, no NPY and MC4R (a melanocortin receptor) double-labeled neurons were observed.

208 double-labeled sections, in which few if any double-labeled neurons were observed.

209 However, only a few double-labeled neurons were occasionally observed after

210 es were present in the injured cortex, while double-labeled neurons were present predominantly in inj

211 In the RVM of rats, many of those double-labeled neurons were retrogradely labeled from th

212 ric acid stimulation, there was a cluster of double-labeled neurons with distinctive large soma in th

213 response to quinine, there was a cluster of double-labeled neurons with much smaller soma in the int

214 ese animals was examined for the presence of double-labeled neurons, i.e., those whose axons had rege

215 colocalized and contained some percentage of double-labeled neurons.

216 ed neurons in the claustrum, as well as many double-labeled neurons.

217 trogen-mediated differences in the number of double-labeled neurons.

218 NST and CeA exhibited significant numbers of double-labeled neurons.

219 d neurons in ipsilateral DEn, including many double-labeled neurons.

220 The double-labeled NMJs were observed in vivo with video-enh

221 caspase-3 and 5-bromo-2'-deoxyuridine (BrdU) double-labeled nuclei were seen in the proliferative reg

222 pha-CreER(T2) : R26R-YFP transgenic mice, we double labeled OPCs and traced their fate in the postnat

223 The double-labeled peptides have amide I' IR spectra that sh

224 No double labeled perikarya were seen in the parabrachial n

225 Double-labeled perikarya for both CTb and proTRH in the

226 From triple-labeled tissue, we found that double-labeled PHA-L (+)/VGluT2 (+) axon terminals forme

227 Fluorescence microscopy in double-labeled PMNs demonstrates that FcgammaRIIA coloca

228 These mGluR1alpha double-labeled populations are not likely to overlap sin

229 In double-labeled preparations using antisera to PHA-L and

230 The powerful double-labeled probe technique was extended to study the

231 A dense plexus of double-labeled prodynorphin/proNKB-ir fibers was found w

232 nation of methods allows for rapid access to double-labeled proteins with a minimum of unnecessary ch

233 m the extent of line shape broadening in the double-labeled proteins.

234 triple-labeled PV(+) /CR(+) /SMI32(+) ; (b) double-labeled PV(+) /CR(+) ; and (c) single-labeled CR(

235 In >250 Cx32/Cx43 single- and double-labeled replicas from 10 CNS regions, no neuronal

236 he lateral geniculate nucleus (LGN) revealed double-labeled retinal ganglion cells (DL RGCs) projecti

237 movement was quantified by image analysis of double-labeled retinas examined with confocal microscopy

238 n a wide system of conditions using the same double-labeled sample from which the FRET efficiency its

239 ing were obtained from natural abundance and double-labeled samples containing [2-(13)C, (15)N]Gly.

240 n in (15)N decoupled 1D or 2D MAS spectra of double-labeled samples.

241 Quantitative analysis of double-labeled sections demonstrated that approximately

242 Analysis of the double-labeled sections indicates that NTPDase2 immunore

243 In double-labeled sections, Glu-ir perikarya within the ter

244 also confirmed by fluorescence microscopy of double-labeled sections, in which few if any double-labe

245 3-7 days, the trigeminal ganglion contained double-labeled, small (11.8 x 8.0 microm), ellipsoid gan

246 mm apart labeled overlapping zones in Pp and double-labeled some cells.

247 A large proportion of double-labeled SON somata were observed in all cases in

248 gher density of synaptic PKMzeta labeling in double-labeled spines correlated with both faster task a

249 These double-labeled spines had larger synapses, as measured b

250 Within this population of double-labeled spines, aged monkeys compared with young

251 The percentage of double-labeled striatopallidal or striatonigral projecti

252 Single- and double-labeled studies using antibodies against CD14, CD

253 coefficients (slopes) between serum PTH and double-labeled surface (P=0.02) or osteoblast surface (P

254 Increased double-labeled surface and mineral apposition rates were

255 rmone (PTH), which is indicated by decreased double-labeled surface and osteoblast surface (P<0.001).

256 most indices of bone formation-including the double-labeled surface, mineralizing surface, mineral ap

257 l nerve and DRG, respectively, the number of double-labeled sympathetic postganglionic neurons was gr

258 ger apoptosis in cycling cells, we performed double-labeled terminal deoxynucleotidyltransferase-medi

259 of calbindin-immunoreactive terminal fibers; double-labeled terminals were documented at high magnifi

260 Further, analysis of double-labeled tissue reveals that the PHA-E and G-10 si

261 ctivated during the hemorrhage stimulus, and double-labeled to identify serotonin (5-hydroxytryptamin

262 In 180 crypt sections double labeled using histone H3 ISH and BrdUrd, 92% of 1

263 ges fetal day (Fd) 89 through adulthood were double labeled using immunofluorescence for short (S) or

264 Buttons were single and double labeled using phalloidin and antibodies to ZO-1,

265 brain for the presence of single labeled or double labeled vagal preganglionic neurons.

266 As expected from these results, using a double-labeled virus, we observed that foci of ICP0 and

267 Double-labeled WGA-HRP/ChAT neurons were found in the pe

268 Double-labeled WGA-HRP/NADPH-d-positive neurons could be

269 entages of efferent neurons were found to be double labeled when using two fluorescent substances inc

270 test this hypothesis, sartorius muscles were double labeled with a fluorescent dye, 4-(4-diethylamino

271 Agarose-embedded sections were double labeled with a panel of antibodies.

272 ot in the MICG, and some of these cells were double labeled with an antiserum to the glial protein S-

273 readily identified in the aorta and could be double labeled with antibodies to CD11c and antigen-pres

274 When mouse retina were double labeled with B16 and anti-alpha-actinin, B16 was

275 ocessed, and frozen; 80-nm cryosections were double labeled with combinations of CCR5, CXCR4, and CD4

276 P2X5 receptors double labeled with differentiated fetal keratinocytes t

277 The retinas were double labeled with either anti-vimentin and anti-long/m

278 clei, hypothalamic, and forebrain areas were double labeled with FG and Fos, the results suggest that

279 Brains were double labeled with fluorescence in situ hybridization o

280 tures that in the human VZ/SVZ, cells can be double labeled with RG markers and calretinin (CalR) and

281 Untagged, recombinant C2A was double-labeled with 13C and 15N, and triple-resonance NM

282 Furthermore, pyknotic O4+ OLs were double-labeled with 4-HNE.

283 eled cells at the hilar/CA3 border also were double-labeled with a neuronal marker (NeuN).

284 emistry of the skin showed CD1a-positive DCs double-labeled with an antibody against DV envelope glyc

285 , ii) single-stained with anti-APP, and iii) double-labeled with anti-APP and AT8.

286 veola-sized vesicular profiles that could be double-labeled with anti-dystrophin and anti-cav-3 antib

287 rgic nature of the TH-i cells, sections were double-labeled with antibodies to dopamine transporter (

288 rols (n=5), c-Fos-positive cells and neurons double-labeled with c-Fos and beta-endorphin, enkephalin

289 Moreover, neurons double-labeled with c-Fos and choline acetyltransferase

290 on (n=5), c-Fos immunoreactivity and neurons double-labeled with c-Fos and either enkephalin or 5-HT

291 Moreover, neurons double-labeled with c-Fos and enkephalin in the rVLM wer

292 More neurons double-labeled with c-Fos and nitric oxide synthase (NOS

293 ulation), c-Fos immunoreactivity and neurons double-labeled with c-Fos, an immediate early gene and t

294 demonstrated that nearly all TH-i cells were double-labeled with DAT, suggesting that they contain th

295 -HT-immunoreactive (5-HT-ir) cells were also double-labeled with FG.

296 % of the remaining Rbpms-positive cells were double-labeled with FG.

297 The IL1beta-IR cells double-labeled with glial fibrillary acidic protein (GFA

298 ically characterized auditory neurons can be double-labeled with HRP and Fos after iontophoretic inje

299 The percentage of GnRH neurons that double-labeled with NMDA-R1 was 2% in prepubertal rats a

300 pale and thick stripes; 10-27% of cells were double labeled, with most located in interpatches.