ライフサイエンスコーパス: doubly labeled

1 ct test for intermolecular isotope transfer, doubly labeled [1(R)-D, 13C3]DHAP and 13C-depleted [1(R)

2 In the reaction of doubly labeled 13C(2,4) mesityl oxide, a 100% scrambling

3 f-principle, we analyzed amino acids using a doubly labeled (15)N(13)C-cell extract as IS.

4 Doubly labeled (2'S,3'R)-[3'-2H1,13C1]-tryptophan was fe

5 he kinetic isotope effect (KIE) observed for doubly labeled AB (k(NH3BH3)/k(ND3BD3) = 3.7) is the pro

6 es were (13)C-labeled to yield singly or/and doubly labeled acetyl-CoA.

7 probe decreased, and the fluorescence of the doubly labeled actin (S265C) was approximately 50% less

8 gle-molecule and ensemble spectroscopies and doubly labeled Acyl-CoA binding protein and double-stran

9 ulation of small to medium-sized neurons was doubly labeled after injections of the tracers into the

10 F NMR studies were carried out on singly and doubly labeled alpha3, alpha6, and alpha10 variants at d

11 nly at alpha, only at beta, or both) and for doubly labeled alphaalpha or betabeta Tm.

12 perimental data were obtained for singly and doubly labeled alphabeta Tm (donor only at alpha, only a

13 taTm were combined in a global analysis with doubly labeled alphabetaTm.

14 d with Co(2+), GTP, and H(2)O(2) to create a doubly labeled and inactive Co(3+)(n(1))-PEPCK-Co(3+)(n(

15 ated by direct distance measurements using a doubly labeled arrestin with one nitroxide in the C-tail

16 ltiple different species of either singly or doubly labeled azurin were formed.

17 sets, each containing microscopy images of a doubly labeled Bacillus subtilis strain.

18 Here, we show that singly and doubly labeled BBL do not aggregate, unfold reversibly,

19 In beta1-null myotubes, FRET efficiencies of doubly labeled beta1a in puncta were similar to those of

20 enditure was determined from the turnover of doubly labeled body water.

21 The EPR analysis of six doubly labeled bR mutants indicates that the cytoplasmic

22 Many doubly labeled (BrdU-FG) cells were also surrounded in t

23 All of the doubly labeled (BrdU-FG) neurons also immunolabeled with

24 d VTA neurons, as well as their fibers, were doubly labeled by striatal injections into the dorsolate

25 8)O-labeled cellobiose, and (13)C- and (18)O-doubly-labeled cellobiose were synthesized and subjected

26 on-electron resonance (DEER) measurements on doubly labeled constructs of CBD12 have shown that the b

27 The doubly labeled cTnC mutant was reconstituted into the th

28 First, using a doubly labeled DNA construct, we show that Pol beta bend

29 and it is even difficult to distinguish the doubly-labeled DNA component from the singly-labeled com

30 py (FCS) system to measure the percentage of doubly labeled dsDNA (PDL) in the total fluorescent dsDN

31 curate measurement of the percentage of such doubly labeled dsDNA component is a critical prerequisit

32 in that is bound to DNA origami; 2), a minor doubly labeled dsDNA subpopulation concealed in a larger

33 nsufficient to measure the percentage of the doubly-labeled dsDNA component in the fluorescent DNA sa

34 itu product analysis by NMR using synthetic, doubly labeled duplex DNA containing (13)C-2 and (15)N(1

35 a 20% increase in pyrene fluorescence of the doubly labeled F-actin but no change in WT actin C374 pr

36 In this study, the pseudotyped doubly labeled fluorescent reporter red/green (R/G)-HIV-

37 ere ringed by 5-HT1A immunoreactivity became doubly labeled following injections of the retrograde tr

38 he majority of c-fos-expressing neurons were doubly labeled for GABA (85 +/- 5%), whereas relatively

39 fused with [1,2-13C2]acetate, malonyl-CoA is doubly labeled from [1,2-13C2]acetate and singly labeled

40 Interspin distances of doubly labeled hCB(1)(T377-E416) peptide reconstituted i

41 In all doubly labeled isoforms, upon S1 binding, one donor-acce

42 rate here a more sensitive method in which a doubly labeled lactate species can be used to measure LD

43 ation-dependent LDL-CE-selective uptake when doubly labeled LDL particles were incubated with SR-BI-e

44 sotope effect for transfer of deuterium from doubly labeled material (kCHOH/kCDOD = 4.88) was within

45 The methodology was evaluated using seven doubly-labeled mutants of myoglobin designed to monitor

46 In the ventrolateral medulla, doubly labeled neurons were concentrated in the area of

47 In the dorsal medulla, doubly labeled neurons were limited to C2 and C3 cell gr

48 in beta-gal transgenic mice, and identified doubly labeled neurons.

49 idine (5BrdU), resulted in the appearance of doubly labeled nuclei in a large percentage of the LREC.

50 teron at the C-12 position on the pathway to doubly labeled patchoulol.

51 d fluorescence measurements suggest that the doubly labeled peptides exist in an equilibrium consisti

52 The doubly labeled peptides have low fluorescence because of

53 In addition, three pairs of doubly labeled proteins were prepared to monitor the int

54 A doubly labeled Proxyl/TOAC(348)-Gtalpha(340-350) was exa

55 nergy transfer efficiency with time when the doubly-labeled pump is mixed with Na+ or K+ demonstrate

56 (15)N-labeled and (15)N, (13)C-doubly-labeled recombinant MUP-I were produced in a bact

57 NMR triple resonance studies of doubly labeled residues within a set of collagen model p

58 (13)C,(15)N-labeled amino acids to prepare a doubly labeled sample.

59 Cleavage of the doubly labeled substrate by HIV protease precludes compl

60 dicates that free 15NH3 is not lost from the doubly labeled substrate during catalysis nor can exogen

61 The kinetics of the appearance of doubly labeled suprabasal cells demonstrate that E7 expr

62 In a glassy trehalose matrix the Tm for the doubly labeled T4 lysozyme was long enough to measure an

63 Doubly labeled [U-(13)C,(15)N]DHFR was obtained from Esc

64 The proof of concept is demonstrated on doubly labeled ubiquitin in HeLa cells.

65 men with RA by using the reference method of doubly labeled water ((2)H(2)(18)O).

66 repeated measures of energy expenditure via doubly labeled water (DLW) along with multiple dual-ener

67 usly proposed EI biomarker equation based on doubly labeled water (DLW) and updated biomarker equatio

68 ltiple-Pass Method Validation Study (n=471), doubly labeled water (DLW) and urinary nitrogen (UN) wer

69 human energy expenditure when measured using doubly labeled water (DLW) but not by respiratory chambe

70 We examine repeated doubly labeled water (DLW) measurements of TEE in 348 ad

71 nergy expenditure (EE) was measured with the doubly labeled water (DLW) method (EE(DLW)).

72 ture (TEE) in patients with SBS by using the doubly labeled water (DLW) method to inform energy needs

73 metabolic ward, investigators have used the doubly labeled water (DLW) method to measure total daily

74 tal energy expenditure estimations using the doubly labeled water (DLW) method were used for this sec

75 iture (TDEE) was measured over 7 d using the doubly labeled water (DLW) method, and self-reported EI

76 intenance and can be determined by using the doubly labeled water (DLW) method.

77 ergy expenditure (TEE) measured by using the doubly labeled water (DLW) method; however, the database

78 7-day accelerometer measurements, at least 1 doubly labeled water (DLW) physical activity level (PAL)

79 24s, two 7-day accelerometer measurements, 1 doubly labeled water (DLW) physical activity level (PAL)

80 ta, Canada (2009-2011), who completed 14-day doubly labeled water (DLW) protocols, 7-day activity dia

81 Few large doubly labeled water (DLW) studies have provided an obje

82 have not validated these methods against the doubly labeled water (DLW) technique for measuring EE.

83 >250 studies on energy metabolism using the doubly labeled water (DLW) technique, approximately 12 f

84 total energy expenditure (TEE) by using the doubly labeled water (DLW) technique.

85 EI biomarkers were developed based on doubly labeled water (DLW) total energy expenditure (TEE

86 e use of energy expenditure (EE) measured by doubly labeled water (DLW), in conjunction with precise

87 tal daily energy expenditure on the basis of doubly labeled water (DLW), resting metabolic rate, the

88 criterion total energy expenditure (TEE) by doubly labeled water (DLW).

89 and males of self-reported TEI and TEE from doubly labeled water (DLW).

90 predict TEE against the reference method of doubly labeled water (DLW).

91 with energy expenditure determined by using doubly labeled water (DLW).

92 (DXA)] and energy expenditure [measured with doubly labeled water (DLW)] from weeks 4-12 and 12-24.

93 ting metabolic rate (RMR) were measured with doubly labeled water (DLW; with and without adjustments

94 epidemiologic studies with EE obtained from doubly labeled water (EE(DLW)) in free-living men.

95 wk of the BD and KD periods was measured by doubly labeled water (EEDLW).

96 otal daily energy expenditure (TEE) by using doubly labeled water (n = 120).

97 TEE was measured by using doubly labeled water (TEE(DLW)), and MEI was measured by

98 Respirometry and doubly labeled water accurately estimate energy expendit

99 ming a classical measurement error model for doubly labeled water and a Berkson error model for the e

100 l activity energy expenditure estimated from doubly labeled water and each physical activity assessme

101 expenditure measured over 10 days at home by doubly labeled water and for 36 hours in the laboratory

102 The application of doubly labeled water and indirect calorimetry to underst

103 mill), physical activity energy expenditure (doubly labeled water and indirect calorimetry), glucose

104 Daily energy expenditure was determined from doubly labeled water and indirect calorimetry, lipolysis

105 ing metabolic rate (RMR) were measured using doubly labeled water and indirect calorimetry, respectiv

106 ing weeks 8 and 16, subjects were dosed with doubly labeled water and maintained physical activity lo

107 Review of doubly labeled water and respiratory chamber studies ide

108 nd fat mass (FM) (4-compartment model), AEE (doubly labeled water and SEE), and regional lean tissue

109 A literature search using terms related to doubly labeled water and TEE was conducted in PubMed, ME

110 Doubly labeled water and urinary nitrogen biomarkers wer

111 In 477 participants aged 18-74 years, doubly labeled water and urinary nitrogen were used as o

112 ed biomarkers of energy and protein intakes: doubly labeled water and urinary nitrogen.

113 a technique that has been validated against doubly labeled water and whole-body calorimetry.

114 E and 22 REE equations were compared against doubly labeled water and whole-room indirect calorimeter

115 Hence, on the basis of the doubly labeled water data and the results of epidemiolog

116 for accuracy by using bootstrap analysis and doubly labeled water data published by other research gr

117 S (total energy expenditure [TEE]/BMR) using doubly labeled water during ultra-endurance competitions

118 metry, and total energy expenditure (TEE) by doubly labeled water in 15 short-stature (height-for-age

119 icles included those that measured TEE using doubly labeled water in adults with a major chronic dise

120 Doubly labeled water is an unbiased reference biomarker

121 alls (ASA24s), four 24-hour urine samples, 1 doubly labeled water measurement (repeated in 104 partic

122 onths later to estimate the stability of the doubly labeled water measurement.

123 iving in Worcester, Massachusetts, completed doubly labeled water measurements and wore an activity m

124 We compared doubly labeled water measurements of TEE among 17 primat

125 Regression equations based on doubly labeled water measurements of TEE appear to be mo

126 (AEE) and PA level were calculated from 7-d doubly labeled water measurements, time spent in light-i

127 With the advent of the doubly labeled water method for determining free-living

128 balance in prepubertal children by using the doubly labeled water method for estimating total energy

129 atabase of total expenditure measured by the doubly labeled water method for males and females aged 8

130 The development of the doubly labeled water method has obviated this problem an

131 The doubly labeled water method now makes it possible to qua

132 and energy expenditure was measured with the doubly labeled water method over each period.

133 ometric and laboratory measures by using the doubly labeled water method to determine each subject's

134 Using the doubly labeled water method to measure total energy expe

135 The doubly labeled water method was used to determine ERs.

136 TEE (measured by the doubly labeled water method), resting metabolic rate (RM

137 s from Los Angeles, California, by using the doubly labeled water method, and the results were compar

138 g studies of total energy expenditure by the doubly labeled water method, measurement of total body w

139 asured with the use of accelerometry and the doubly labeled water method, respectively.

140 Using the doubly labeled water method, we measured daily energy ex

141 energy expenditure (TEE) was measured by the doubly labeled water method.

142 nergy expenditure (TDEE) was measured by the doubly labeled water method.

143 orimetry and daily energy expenditure by the doubly labeled water method.

144 studied by several research groups using the doubly labeled water method.

145 32 SQCP; n = 32 control group) by using the doubly labeled water method.

146 based on energy expenditure derived from the doubly labeled water method.

147 Total energy expenditure was measured by the doubly labeled water method.

148 rge enclosures in a nature reserve using the doubly labeled water method.

149 home setting over a 10-d period by using the doubly labeled water method.

150 penditure and TBF were assessed by using the doubly labeled water method.

151 rgy expenditure (TEE) was measured using the doubly labeled water method; body composition (fat mass,

152 culated as the total energy expenditure from doubly labeled water minus the resting energy expenditur

153 single, group mean energy adjustment (using doubly labeled water or physical activity) reduced the v

154 ilated hood, and total energy expenditure by doubly labeled water prior to the overeating and weight

155 ative Dietary Modification Trial completed a doubly labeled water protocol (energy biomarker), 24-hou

156 In addition, doubly labeled water studies have found a significant ne

157 A review of cross-sectional data from doubly labeled water studies in adults provided support

158 otal energy requirements were estimated from doubly labeled water studies of total energy expenditure

159 Evidence from doubly labeled water studies suggests that these diets a

160 TEE was based on the results of doubly labeled water studies, and the TEE results were p

161 ine energy requirements were determined by a doubly labeled water study and caloric titration to weig

162 ited validation of these methods against the doubly labeled water technique for determining energy ex

163 The doubly labeled water technique is an isotope-based metho

164 measuring PA, such as accelerometry and the doubly labeled water technique, were developed only with

165 7 days in free-living conditions, using the doubly labeled water technique.

166 terrestrial vertebrates as determined by the doubly labeled water technique.

167 energy expenditure measured with the use of doubly labeled water to energy intake from dietary recor

168 Energy expenditure with the use of doubly labeled water was done contemporaneously with the

169 ced the variance in fat intake (p<0.05) when doubly labeled water was used to first correct energy in

170 which valid reference biomarkers for energy (doubly labeled water) and protein (urinary nitrogen), to

171 ere we report that total energy expenditure (doubly labeled water) measured in 89 nondiabetic Pima In

172 expenditure (assessed over 2 weeks by using doubly labeled water) minus resting metabolic rate (meas

173 tion level, and estimated energy intake from doubly labeled water) were used for evaluation against e

174 TEE (measured by doubly labeled water), food intake, activity patterns (m

175 ergy X-ray absorptiometry), free-living TEE (doubly labeled water), Tecumseh Occupational Activity Qu

176 ical-activity-related energy expenditure (by doubly labeled water).

177 red total energy expenditure, measured using doubly labeled water, against physical activity, measure

178 lorimetry, total energy expenditure (TEE) by doubly labeled water, and activity energy expenditure (A

179 energy expenditure were also available from doubly labeled water, and basal energy expenditure was e

180 nge, total energy expenditure (TEE) by using doubly labeled water, and body composition by using dual

181 EE was measured by using doubly labeled water, and food and drink intake was reco

182 DEE was measured by using doubly labeled water, BEE by using respirometry, and bod

183 ily energy expenditure (TEE) was measured by doubly labeled water, body composition by dual-energy X-

184 iture (EE), measured by 24-h calorimetry and doubly labeled water, differed in normal-weight-for-heig

185 sical activity were assessed with the use of doubly labeled water, exercise energy economy and muscle

186 e measurements of EE by 24-h calorimetry and doubly labeled water, of muscle metabolism by (31)P nucl

187 TEE was measured with doubly labeled water, sleeping metabolic rate with respi

188 energy expenditures measured with the use of doubly labeled water, the former consistently underestim

189 tivity energy expenditure, assessed by using doubly labeled water, was associated with a reduced decl

190 Total daily EE (TDEE) was measured by using doubly labeled water.

191 ily energy expenditure was assessed by using doubly labeled water.

192 and physical activity level were measured by doubly labeled water.

193 gy expenditure (TEE) was determined by using doubly labeled water.

194 M, FM, and total energy expenditure (TEE) by doubly labeled water.

195 xpenditure from body composition changes and doubly labeled water.

196 measured by indirect calorimetry and TEE by doubly labeled water.

197 TEE was measured by doubly labeled water.

198 ich energy expenditure was measured by using doubly labeled water.

199 0% of total energy expenditure determined by doubly labeled water.

200 rmined by using respiratory gas exchange and doubly labeled water.

201 expenditure was assessed over 2 weeks using doubly labeled water.

202 ctivity-related energy expenditure (AREE) by doubly labeled water; and dietary protein intake by self

203 Energy expenditure was measured by doubly-labeled water and whole-room indirect calorimetry

204 ion of energy expenditure in humans, we used doubly-labeled water methodology to determine whether fa

205 llows one to measure energy expenditure via "doubly labeled" water (2H(2)O + H(2)18O), using small sa

206 ectra of the triple-helix glucan, laminarin, doubly labeled with 1-aminopyrene as donor probe and flu

207 ed here was produced in Escherichia coli and doubly labeled with 13C and 15N.

208 r aqueous solutions of hemoglobin singly and doubly labeled with a nitroxide and mercury(II) ion at c

209 single-stranded oligonucleotide probes were doubly labeled with adjacent thiazole orange derivatives

210 Sections were doubly labeled with antibodies directed against AR and o

211 None of the progenitors was doubly labeled with any combination of markers character

212 d that swellings labeled with BDA, sometimes doubly labeled with BDA and (3)H-leucine, were in close

213 The disaccharide was doubly labeled with biotin and Cy5.

214 stem cells and human mesenchymal stem cells doubly labeled with ferumoxides and beta-galactosidase a

215 A calmodulin (CaM) mutant (T34,110C-CaM) doubly labeled with fluorescence probes AlexaFluor 488 a

216 A CaM mutant (CaM-T34C-T110C) was doubly labeled with fluorescent probes AlexaFlour 488 an

217 Eight T4 lysozyme (T4L) mutants, doubly labeled with methanethiosulfonate spin-label (MTS