ライフサイエンスコーパス: eIF4B

1 eIF4B also helps to organize the assembly of the transla

2 eIF4B and eIF4H were shown to stimulate the helicase act

3 eIF4B and eIFiso4G exhibited competitive binding to PABP

4 eIF4B has been implicated in attachment of the 43 S prei

5 eIF4B interacted with PABP in a phosphorylation state-sp

6 eIF4B is required for the translation of mRNAs with stru

7 eIF4B stimulates the helicase activity of eIF4A, thereby

8 eIF4B was rapidly phosphorylated within 20 h of germinat

9 eIF4B, eIF4A, and Ded1 mutations also preferentially imp

10 eukaryotic translation initiation factor 4B (eIF4B) as a MELK-interacting protein during mitosis and

11 the role of eukaryotic initiation factor 4B (eIF4B) in this process and identify 10-fold more RNA bin

12 eukaryotic translation initiation factor 4B (eIF4B) is a cleavage site preferred by caspase-2 not onl

13 Eukaryotic translation initiation factor 4B (eIF4B) is a cofactor for eIF4A but also might function i

14 Eukaryotic initiation factor 4B (eIF4B) is a multidomain protein with a range of activiti

15 rylation of eukaryotic initiation factor 4B (eIF4B) on S406.

16 eukaryotic translation initiation factor 4B (eIF4B) played a key role, as the mutation of eIF4B at se

17 perate with eukaryotic initiation factor 4B (eIF4B) to control translation in a manner that is respon

18 eukaryotic translation initiation factor 4B (eIF4B), an integral component of the translation initiat

19 eukaryotic translation initiation factor 4B (eIF4B), increased its assembly into translation initiati

20 eukaryotic translation initiation factor 4B (eIF4B).

21 Consistent with these data, we locate an eIF4B binding site upstream of the stem-loop structure i

22 showed enhanced phosphorylation of 4EBP1 and eIF4B; these findings indicated activation of the mTOR p

23 Addition of eIF(iso)4G and eIF4B that had also been phosphorylated in vitro further

24 th eukaryotic initiation factor (eIF) 4G and eIF4B.

25 cooperation between closed-loop assembly and eIF4B/helicase functions.

26 eIF2beta and eIF4B, present as highly phosphorylated isoforms during

27 horylation state of eIF2alpha, eIF2beta, and eIF4B is developmentally regulated in a way that correla

28 ding complex eIF4F and the factors eIF4A and eIF4B are required for binding of 43S complexes (compris

29 f a stable ternary complex between eIF4A and eIF4B.

30 n, and binding domains for eIF4E, eIF4A, and eIF4B; (ii) eIF4G601-1488, which contains an additional

31 the formation of SGs and restored eIF4E and eIF4B levels in CA1.

32 ompounds, the cap-binding protein eIF4E, and eIF4B, suggesting that remodeling of the eIF4F complex w

33 lexed with two accessory proteins, eIF4G and eIF4B.

34 ypeptide chain initiation factors eIF4GI and eIF4B, a phenomenon previously observed in cells induced

35 at the p53-regulated cleavages of eIF4GI and eIF4B, as well as the overall inhibition of protein synt

36 support the notion that eIF4G, eIFiso4G, and eIF4B interact with distinct molecules of PABP to increa

37 eukaryotic initiation factor (eIF)-iso4G and eIF4B, and these interactions increased the poly(A) bind

38 or protein processing, amyloid-beta load and eIF4B phosphorylation, whereas spatial and associative l

39 PABP and eIF4B compete with eIF4A for binding eIFiso4G in the abs

40 on factor (eIF) 4G (in yeast and plants) and eIF4B (in plants), a functional consequence of which is

41 Poly(A)-binding protein and eIF4B mainly affect the eIF4F/TEV association rate.

42 lysosomal degradation of eIF4B protein; and eIF4B inhibits IAV replication by upregulating expressio

43 Using the somatic mutation rate and eIF4B protein level, we identified three groups with dif

44 y eIF4E at multiple copies per ribosome, and eIF4B at approximately one copy per ribosome.

45 s of MEK, but not of TORC1/2, blocked S6 and eIF4B phosphorylation and gamma2 overexpression.

46 nase (S6K) and its downstream targets S6 and eIF4B.

47 eads to increased phosphorylation of S6K and eIF4B, boosting mRNA translation.

48 The two Arabidopsis eIF4B isoforms, as well as native and recombinant wheat

49 ant decrease in the binding affinity between eIF4B and BC RNA translational repressors, enabling the

50 F4B and PABP but not the interaction between eIF4B and eIF4A or eIFiso4G, demonstrating that the effe

51 Zinc promoted the interaction between eIF4B and PABP but not the interaction between eIF4B and

52 erentially promoting the interaction between eIF4B and PABP.

53 inding to eIFiso4G was also observed between eIF4B and PABP.

54 ignaling to p70S6K1; phosphorylation of both eIF4B and PDCD4 was additionally required.

55 However, when both eIF4B and PABP were present, not only was the energy bar

56 imulated homodimerization of eIF4B and bound eIF4B with a Kd of 19.7 nM.

57 cible ISG15 protein expression is blocked by eIF4B or eIF3A knockdown, establishing a requirement for

58 e helicase activity of eIF4A is modulated by eIF4B, eIF4H, or as a subunit of eIF4F.

59 mino acids each) of Saccharomyces cerevisiae eIF4B (yeIF4B) compose the region most critically requir

60 especially in the presence of its "cofactor" eIF4B, promotes ATP-dependent unwinding of localised sec

61 upporting a central role for these conserved eIF4B domains in facilitating interaction with other com

62 ure in histone mRNAs and show that decreased eIF4B expression alters histone mRNA turnover and delays

63 ied recombinant caspase-3 is able to degrade eIF4B and eIF3(p35) in vitro, producing fragments of the

64 Upon neuronal stimulation, synapto-dendritic eIF4B is dephosphorylated at serine 406 in a rapid proce

65 mRNA levels, consistent with ERK-dependent, eIF4B-mediated translation initiation of the stem-looped

66 rcome host innate immunity by downregulating eIF4B protein.

67 to phosphorylate eIF2alpha, eIF2beta, eIF3c, eIF4B, eIF5, and histone deacetylase 2B but did not phos

68 The combinations of eIF4A, eIF4A + eIF4B, eIF4A + eIF4H, and eIF4F showed differences in th

69 tivity and RNA helicase activity of (eIF4A + eIF4B + eIF-iso4F) complex.

70 tiation complexes incubated with ATP, eIF4A, eIF4B and eIF4F bind exclusively to the cap-proximal reg

71 canning rate of the complex formed by eIF4A, eIF4B, and eIF4F or eIF-(iso)4F and increase the rate of

72 In unstressed cells, eIF4A, eIF4B, and Ded1 primarily targeted the 5' ends of mRNAs.

73 sulting complex requires eIF1, eIF1A, eIF4A, eIF4B and eIF4F to bind to a messenger RNA and to scan t

74 require the initiation factors eIF3, eIF4A, eIF4B, and eIF4F and translation of these mRNAs was not

75 presence of active helicase factors (eIF4A, eIF4B, eIF4F and ATP).

76 nosine triphosphate (ATP) or factors (eIF4A, eIF4B, eIF4F) associated with ATP hydrolysis.

77 nslation initiation of natural mRNAs, eIF4A, eIF4B, and eIF4F.

78 The helicase complex, consisting of eIF4A, eIF4B, and ATP, stimulated BTE binding with eIF4G601-119

79 ro RNA-dependent ATPase activities of eIF4A, eIF4B, and eIF4F.

80 ganization but both can interact with eIF4A, eIF4B, eIF4E isoforms, and the poly(A)-binding protein.

81 0 nm), (ii) the helicase complex eIF4F-eIF4A-eIF4B-ATP increases 40S subunit binding (Kd = 120 +/- 10

82 ng (eukaryotic initiation factor 4A [eIF4A], eIF4B, and Ded1), indicating a common mechanism of trans

83 tivities, but these are stimulated by eIF4G, eIF4B, and eIF4H.

84 ll of them require eIF2, eIF3, eIF4A, eIF4G, eIF4B, eIF1A, and a single ITAF, poly(C) binding protein

85 The accessory proteins eIF4G, eIF4B, and eIF4H enhance the duplex unwinding activity o

86 proximately 2.4-fold faster for the eIFiso4F.eIF4B complex compared with our previous studies of eIFi

87 escence stopped-flow studies of the eIFiso4F.eIF4B protein complex with two m(7)G cap analogues show

88 Either eIF4B or eIF4H stimulated the initial rate and amplitude

89 s markedly reduced in the presence of either eIF4B or PABP.

90 eIF4A to the IRES in conjunction with either eIF4B or with the carboxy-terminal third of eIF4G.

91 However, either eliminating eIF4B or inactivating eIF4A preferentially impacts mRNAs

92 A or Ded1 activity revealed that eliminating eIF4B reduces the relative translational efficiencies of

93 sphorylation of eukaryotic initiation factor eIF4B, which is critical to unwind its structured 5' unt

94 d its downstream effector, initiation factor eIF4B.

95 Depletion of the related factor eIF4B did not affect Vhs activity.

96 nt phosphorylation of the translation factor eIF4B.

97 the presence of a second initiation factor, eIF4B.

98 a eukaryotic translation initiation factor, eIF4B.

99 wnstream mRNA translation initiation factors eIF4B and 4EBP1, as well as elevated phosphorylation of

100 omain of PABP that binds translation factors eIF4B and eRF3 from the N-terminal RNA-binding domain of

101 A single interaction domain for eIF4B is present within a 32-amino acid region represent

102 r the first time, the interaction domain for eIF4B.

103 effects and define a key regulatory role for eIF4B as a common mediator and integrator of IFN-generat

104 indings reveal an eIF4A-independent role for eIF4B in addition to its function as eIF4A cofactor in p

105 identify 10-fold more RNA binding sites for eIF4B in tumour cells from patients with diffuse large B

106 F4H is not able to completely substitute for eIF4B in duplex unwinding.

107 ctively, these data provide insight into how eIF4B promotes tumorigenesis.

108 A simple model of how eIF4B or eIF4H affects the duplex unwinding mechanism of

109 These findings link changes in eIF4B and eIF4E to SG induction in regions vulnerable to

110 The decrease in eIF4B level was attributed to lysosomal degradation of e

111 tion of its translational targets, including eIF4B, which is then recruited into the complex in a pho

112 esults indicated that ORF45/RSK axis-induced eIF4B phosphorylation is involved in translational regul

113 Our data also demonstrate that IFN-inducible eIF4B activity and IFN-stimulated gene 15 protein (ISG15

114 IF3, and eIF4F were required for initiation; eIF4B and to a lesser extent the pyrimidine tract-bindin

115 The rapamycin and U1026 doubly insensitive eIF4B phosphorylation was induced during KSHV reactivati

116 Ribosome profiling of mutants lacking eIF4B or with impaired eIF4A or Ded1 activity revealed t

117 Mammalian eIF4B also stimulates eIF4A activity, but this function

118 Mammalian eIF4B is a constitutive dimer; however, conflicting repo

119 The ORF45/RSK-mediated eIF4B phosphorylation was distinguishable from that caus

120 Our study thus defines a MELK-eIF4B signaling axis that regulates protein synthesis du

121 We further show that the MELK-eIF4B signaling axis regulates protein synthesis during

122 hosphorylation state-specific manner; native eIF4B increased the RNA binding activity specifically of

123 hown to stimulate the in vitro activities of eIF4B and eIF4F in globin synthesis, as well as the in v

124 The addition of eIF4B and poly(A)-binding protein enhanced the associati

125 The addition of eIF4B resulted in a change in binding affinity for ATP,

126 The binding of eIF4B either prior to or after hydrolysis allows for inc

127 osphorylation is required for the binding of eIF4B to the eIF3 translation initiation complex.

128 protein (4E-BP1), and specific cleavages of eIF4B and eIF2alpha.

129 V infection induces lysosomal degradation of eIF4B protein; and eIF4B inhibits IAV replication by upr

130 l was attributed to lysosomal degradation of eIF4B, which was induced by viral NS1 protein.

131 he organization of the functional domains of eIF4B from wheat has been investigated.

132 The kinetic effects of eIF4B, PABP, and wheat germ eIFiso4F with two mRNA cap a

133 Ectopic expression of eIF4B but not its phosphorylation-deficient mutant form

134 Although the function of eIF4B is conserved among plants, animals, and yeast, eIF

135 in a better understanding of the function of eIF4B, the two isoforms from Arabidopsis (Arabidopsis th

136 Zinc stimulated homodimerization of eIF4B and bound eIF4B with a Kd of 19.7 nM.

137 en demonstrated to function independently of eIF4B as an ATP-dependent RNA helicase.

138 ould facilitate the multiple interactions of eIF4B with mRNA as well as other initiation factors (eIF

139 We examined the isoforms of eIF4B and the alpha and beta subunits of eIF2 during the

140 eIF4B) played a key role, as the mutation of eIF4B at serine 422 (S422R) or deletion of the BACE1 5'U

141 plication, and conversely, overexpression of eIF4B markedly inhibited the viral replication.

142 LT treatment diminishes phosphorylation of eIF4B, eIF4E, and rpS6, critical components of the intra

143 .m(7)GpppG, respectively, in the presence of eIF4B and PABP.

144 only a slight dependence on the presence of eIF4B isoforms, whereas rabbit beta-hemoglobin mRNA and

145 ghtly processive helicase in the presence of eIF4B or eIF4H.

146 on of eIF4A with eIFiso4G in the presence of eIF4B or PABP.

147 Our finding illustrates a critical role of eIF4B in the host innate immune response and provides no

148 global rates of protein synthesis to that of eIF4B and PDCD4.

149 lation assays to measure their dependence on eIF4B and eIF4F isoforms.

150 us RNA displayed the strongest dependence on eIF4B.

151 Ded1/Dbp1 type of helicase than on eIF4A or eIF4B.

152 supplemented with eIF4E, eIFiso4E, eIF4A, or eIF4B.

153 and its interaction with eIF4G, eIFiso4G, or eIF4B.

154 Inactivation of MELK or eIF4B results in reduced protein synthesis of MCL1, whic

155 iso4G, an interaction not reported for other eIF4B proteins.

156 MELK phosphorylates eIF4B at Ser406, a modification found to be most robust

157 (p70S6K1), which subsequently phosphorylates eIF4B, and programmed cell death 4 (PDCD4), which seques

158 Plant eIF4B contains three RNA binding domains, one more than

159 Plant eIF4B was found by ultracentrifugation and light scatter

160 onflicting reports have suggested that plant eIF4B may exist as a monomer or a dimer.

161 eIF4E, eIF4A, Mnk1, poly(A)-binding protein, eIF4B, and eIF3 were not bound by Hsp27 and were not rec

162 14-fold more effective than was recombinant eIF4B, whereas eIF4F promoted the cooperative binding of

163 We found a new, highly regulated eIF4B/-3 binding site within the HEAT-1/-2 interdomain l

164 ein synthesis activated by the PI3K-PDK1-RSK-eIF4B axis as the biologically relevant signaling cascad

165 endent on activation of the S6K/eIF4B or RSK/eIF4B pathway.

166 nitors is dependent on activation of the S6K/eIF4B or RSK/eIF4B pathway.

167 Silencing eIF4B expression in A549 cells significantly promoted IA

168 the combined treatment completely suppressed eIF4B phosphorylation and decreased translation initiati

169 at stimulating eIF4A unwinding activity than eIF4B, implying that eIF4H is not able to completely sub

170 er identical conditions, we demonstrate that eIF4B couples the ATP hydrolysis cycle of eIF4A with str

171 Furthermore, we demonstrated that eIF4B regulated the expression of interferon-induced tra

172 s-linking and immunoprecipitation, find that eIF4B binds the entire length of mRNA transcripts.

173 of protein synthesis and we report here that eIF4B, the p35 subunit of eIF3, and minor proportions of

174 These results support the notion that eIF4B functions by organizing multiple components of the

175 Importantly, we observed that eIF4B knockdown transgenic mice were more susceptible to

176 Taken together, our findings reveal that eIF4B plays an important role in host defense against IA

177 These studies show that eIF4B and PABP enhance the interaction with the cap and

178 In this study, we show that eIF4B from wheat can form a dimer and we identify the re

179 Our data show that eIF4B is phosphorylated on Ser422 during treatment of se

180 These results suggest that eIF4B has some effects on mRNA discrimination during ini

181 Moreover, the eIF4B and PABP binding domains overlap.

182 Zinc increased the activity of the eIF4B C-terminal RNA-binding domain specifically.

183 We report here the solution structure of the eIF4B RNA recognition motif (RRM) domain.

184 tures associated with hyperdependence on the eIF4B/helicases.

185 minal eIF4A binding domain overlaps with the eIF4B and PABP binding domains.

186 via hnRNPK and enhances translation through eIF4B, a general translation initiation factor.

187 These C-loops bind to eIF4B and prevent the factor's interaction with 18S rRNA

188 s of expression in given tissues relative to eIF4B.

189 ts well-documented role in mRNA translation, eIF4B additionally interacts with proteins associated wi

190 To ensure efficient duplex unwinding, eIF4B and eIF4G cooperatively activate the duplex unwind

191 BC RNA translational control, mediated via eIF4B phosphorylation status, couples neuronal activity

192 f and a C-terminal RNA binding domain, wheat eIF4B contains a novel N-terminal RNA binding domain tha

193 n the N-terminal RNA binding domain in wheat eIF4B is required for interaction with eIFiso4G, an inte

194 rms, as well as native and recombinant wheat eIF4B, showed similar responses in the translation assay

195 Our observations suggest that wheat eIF4B can dimerize but requires zinc.

196 shed the ability of eIFiso4G to compete with eIF4B in binding to their overlapping binding sites in P

197 RNA helicase that works in conjunction with eIF4B, eIF4H, or as a subunit of eIF4F to unwind seconda

198 4H shares a region of sequence homology with eIF4B, and it appears to be functionally similar in that

199 ction of PABP such that the interaction with eIF4B is preferred over eIFiso4G.

200 In plants, PABP also interacts with eIF4B, a factor that assists eIF4F function.

201 f p110 with p90, p135 with p33, and p33 with eIF4B.

202 th an N-proximal sequence in human and yeast eIF4B.

203 acking in yeast, making it unclear how yeast eIF4B (yeIF4B/Tif3) stimulates translation.

204 ne more than reported for mammalian or yeast eIF4B, and each domain exhibits a preference for purine-

205 conserved among plants, animals, and yeast, eIF4B is one of the least conserved of initiation factor

206 conserved among plants, animals, and yeast, eIF4B is one of the least conserved of initiation factor