ライフサイエンスコーパス: edit

1 ble, drug loadable, and suitable for genetic editing.

2 icircles bearing guide RNAs (gRNAs) for mRNA editing.

3 ntaining minimal Cas9-independent off-target editing.

4 th in meiotic and post-meiotic germ cell RNA editing.

5 ibited normal expression patterns after gene editing.

6 ere modified, with no evidence of off-target editing.

7 otecting pre-mRNA upon KPAF3 displacement by editing.

8 ng including checkpoint inhibitors, and gene editing.

9 he arsenal of enzymes used in precise genome editing.

10 similar to known negative regulators of RNA editing.

11 A-mediated or Csy4-mediated multiplex genome editing.

12 e, constraining the sequences accessible for editing.

13 platform for eukaryotic genome and epigenome editing.

14 enough to be packaged into an AAV for genome editing.

15 ign of guide RNAs for ADAR-mediated RNA base editing.

16 ction, RNA interference and potentially gene editing.

17 ing corrected lines by genome- and epigenome-editing.

18 nal mapping, gene expression, silencing, and editing.

19 e, dogs, and human cells through CRISPR/Cas9 editing.

20 DNA target site during genome and epigenome editing.

21 ed pluripotent stem cells by CRISPR/Cas9gene editing.

22 2) depends on adr-1, a gene important to RNA editing.

23 ns in Arabidopsis At2OGO by CRISPR/Cas9 gene editing.

24 to T*A, in cellular DNA for precision genome editing.

25 significantly muffle the single-nucleic-acid editing ability of the dCas13a RNA-editing system.

26 king of edited cells (BAR-Seq) and show that editing activates p53, which substantially shrinks the H

27 shown to have reduced off-target RNA and DNA editing activities(5,6).

28 hes a critical role for ADAR1 and its A-to-I editing activity during cell fate transitions and deline

29 cer (CRC) cells, thus displaying high genome-editing activity in vitro.

30 Creating accurate maps of A-to-I RNA editing activity is vital to improving our understanding

31 Furthermore, loss of Adar RNA editing activity leads to innate immune induction, indic

32 plored many approaches to improve the genome editing activity of the CRISPR-Cas system and deliver it

33 We propose that the cross-editing activity of ThrRS is evolutionarily conserved an

34 te that these ADAR2 variants lead to reduced editing activity on a known ADAR2 substrate.

35 Editing activity was sensitive to addition of zinc aceta

36 eaminase domain which is important for ADAR1 editing activity.

37 ine-to-inosine activity and can specifically edit adenosines in a known substrate.

38 ownstream 'omics' technologies reflective of edited affects, such as metabolomics, need to be used in

39 zation of the degranulation profiles of both edited and non-edited mast cells, offering a consistent

40 rt palindromic repeat (CRISPR)-mediated gene editing and acetate supplementation of the culture media

41 and to identify potential targets for genome editing and breeding.

42 unintended consequences including off-target editing and chromosomal translocations.

43 reduced repertoire diversity, decreased BCR editing and developmental arrest of immature B cells, re

44 Here, we have combined CRISPR gene editing and engineered separation-of-function mutants to

45 his version does not include post-acceptance editing and formatting.

46 - and development-specific regulation of RNA editing and identify a molecular mechanism that regulate

47 ain proteins, ADAD1 and ADAD2, on testis RNA editing and male germ cell differentiation.

48 CRISPR-Cas gene editing and messenger RNA-based protein replacement ther

49 s the recent applications of tRNAs in genome editing and microbiome sequencing.

50 from functional experiments, such as genome editing and reporter assays.

51 ts and a combination of CRISPR-mediated gene editing and RNAi-mediated gene silencing in human cells,

52 Using both locus-specific genetic editing and simultaneous epigenetic silencing of multipl

53 also discuss the importance of NMD for gene editing and tumor evolution, and how inhibiting NMD may

54 h 3' adenylation, uridine insertion/deletion editing, and 3' A/U-tailing.

55 extends dCas9 residence times, delays genome editing, and alters the balance between indel formation

56 s9-dependent and -independent DNA off-target editing, and in transcriptome-wide RNA off-target editin

57 NNGG PAM, displays high activity for genome editing, and is compact enough to be packaged into an AA

58 er-free DNA in rice using CRISPR-Cas9 genome editing, and offer a promising strategy for genetic impr

59 mental immunology, genetic engineering, gene editing, and synthetic biology exponentially expand oppo

60 efSaCas9 can be broadly used in genome-editing applications that require high fidelity.

61 ion of regulatory regions and multiplex base editing applications.

62 system has been widely used for various gene editing applications.

63 We show here, using a gene-editing approach, that a point mutation that eliminates

64 NPs, could not be reached with a single gene-editing approach.

65 This protocol thus expands CRISPR-based gene editing approaches beyond models of robust T cell activa

66 Future trials should use superior gene editing approaches to improve therapeutic efficacy.

67 ew focuses on new state-of-the-art epigenome editing approaches to modify the epigenome of neoplasms

68 d flexibility, assays to identify off-target editing are becoming more comprehensive and sensitive.

69 icipating and verifying the result of genome editing are essential for the success for all applicatio

70 Advances in gene editing are leading to new medical interventions where p

71 ts with targeted DNA modifications, and gene edits are transmitted to the next generation.

72 argets of RNA-binding proteins identified by editing) as an approach to identify signaling pathways t

73 ploy conserved peroxisomal beta-oxidation to edit ascarosides and change their message.

74 reased in adr-1 mutants due to deficient RNA editing at a single adenosine in their 3'-UTR.

75 discoveries illuminate base editing, enable editing at previously intractable targets, and provide n

76 atients resulted in durable engraftment with edits at all three genomic loci.

77 We observed frequent on-target cytosine base edits at the BCL11A erythroid enhancer at +58 with few i

78 e, this model is also not amenable to genome-editing based therapeutic approaches.

79 with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or doubl

80 ing to as high as 50% and improves multiplex editing by 5- to 10-fold in E. coli, while PapRecT enabl

81 nucleolytic processing by TREX1 and cytosine editing by APOBEC3B.

82 Genome editing by CRISPR (clustered regularly interspaced short

83 ng, and in transcriptome-wide RNA off-target editing can be ameliorated by the introduction of an add

84 l CRISPR-Cas9 homology-directed repair, base editing can correct point mutations without supplying a

85 Gene editing can rapidly improve a range of crop traits, incl

86 ions, and major efforts have broadened their editing capabilities, expanded their targeting scope and

87 , and provide new base editors with improved editing capabilities.

88 ed deaminase (AID) and apolipoprotein B mRNA editing catalytic polypeptide-like (APOBEC) mutagenesis

89 Using multiple gene-edited cell lines and patient-derived samples, we demons

90 cking retains target protein dosages in gene-edited cell populations and expands gene editing to chro

91 y a barcoding strategy to clonal tracking of edited cells (BAR-Seq) and show that editing activates p

92 ngineering a selectable marker to enrich for edited cells.

93 e in hematochimeric mice, although engrafted edited clones preserve multilineage and self-renewing ca

94 While editing concludes in the 5' region, KPAF1/2 dimer induce

95 roduction of this minor allele SNP by genome editing confirmed its functionality in depressing GATA6

96 Adenosine (A) to inosine (I) RNA editing contributes to transcript diversity and modulate

97 redited in thyroid tumors, and its levels of editing correlate with a worse progression-free survival

98 Using CRISPR/Cas9-mediated gene editing, coupled with endocrine cell differentiation str

99 cyl flux around the phosphatidylcholine acyl editing cycle was the largest acyl flux reaction in wild

100 We crossed one genome-edited dairy bull, homozygous for the dominant P(C) Celt

101 The oxidized Pol gamma becomes editing-deficient, displaying a 20-fold elevated mutatio

102 Here we report two methods to generate gene-edited dicotyledonous plants through de novo meristem in

103 culture species, and the development of gene-edited, disease-resistant fish.

104 The present CRISPR/Cas9 gene editing dogma for single guide RNA (sgRNA) delivery is b

105 genes, ribosomal protein S12 (RPS12), the 5' editing domain of NADH dehydrogenase subunit 7 (ND7 5'),

106 Genome-edited donor-derived allogeneic anti-CD19 chimeric antig

107 licing, while the second major mechanism-RNA editing due to post-transcriptional changes of individua

108 rus that significantly improve citrus genome editing efficacy.

109 programmable A*T to G*C point mutations but editing efficiencies can be low at challenging loci in p

110 in cells and allows for titratable levels of editing efficiency and spatial patterning via selective

111 cles with polyglutamic acid further improves editing efficiency by approximately twofold, reduces tox

112 are 46 RNA editing loci with an average RNA editing efficiency of 63%.

113 single gene, the lipoMSN achieved a 54% gene-editing efficiency, besting the state-of-art Lipofectami

114 be optimized, and challenges regarding their editing efficiency, specificity and immunogenicity must

115 , but this comes at the expense of on-target editing efficiency.

116 hat regulates ADAR substrate recognition and editing efficiency.

117 crRNA for Cas12a and obtained a much higher editing efficiency.

118 e for its cognate PAM promotes higher genome-editing efficiency.

119 These discoveries illuminate base editing, enable editing at previously intractable target

120 Prime editing enables diverse genomic alterations to be writte

121 DYW-deaminase domains act as the editing enzyme.

122 In addition, the genome editing enzymes themselves need to be optimized, and cha

123 Non-canonical and C-to-U RNA-editing events are enriched inside and/or adjacent to MN

124 djacent to MNRs, while all categories of RNA-editing events are under-represented in DNRs.

125 However, elucidation of RNA editing events at transcriptome-wide level requires incr

126 discusses how to anticipate and detect those editing events by a combination of assays to capture all

127 he details of uridine insertion and deletion editing events upon the kDNA transcriptome.

128 scellaneous genomic features, especially RNA editing events.

129 e that the Foxp3 locus can be epigenetically edited ex vivo to generate stable therapeutic iTregs.

130 is an important consideration for any genome editing experiment, and a number of Cas9 variants have b

131 However, CRISPR/Cas9 edited F(0) animals too often demonstrate variable pheno

132 SAMMI also offers a wide array of manual map editing features.

133 as) nucleases have revolutionized the genome editing field.

134 ation of MTV in 105 (76%) scans, with simple editing for a satisfactory result in additionally 20% of

135 ++) and HiFi-Sc(++) extend the use of CRISPR editing for diverse applications.

136 -cell RNA-sequencing and CRISPR-Cas9 barcode editing for elucidating developmental lineages at the wh

137 HSPCs as a feasible alternative to nuclease editing for HSC-targeted therapeutic genome modification

138 eptibility target has implications in genome editing for novel plant resistance against devastating H

139 tensive uridine insertion/deletion (U-indel) editing for their maturation.

140 therefore prove beneficial for ex vivo gene editing, for enhanced platelet production, and for the i

141 ted later and retained relatively higher RNA editing frequency.

142 RNA editing generates modifications to the RNA sequences, th

143 an be used in experimental disease models to edit genomes and to control gene expression levels throu

144 nCas9 mouse allows robust and tunable genome editing granting flexibility, speed and uniformity at le

145 transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalia

146 The clinical application of CRISPR-Cas9 gene editing has been eagerly awaited since the first descrip

147 Genome editing has powerful applications in research, healthcar

148 CRISPR/Cas9 genome editing has revolutionized functional genomics in verteb

149 Although recent improvements in genome editing have made it possible to directly modify the tar

150 of the NANOS2 gene generated by CRISPR-Cas9 editing have testes that are germline ablated but otherw

151 recent advances, including CRISPR-based gene editing, have made possible systematic screens for synth

152 reticulum (ER) in mouse AD models and genome-edited human AD iPS cell-derived neurons.

153 that short guide RNAs can also support base editing if they contain cytosines within the deaminase a

154 We found that A-to-I editing impedes MDA5 sensing and sequestration of dsRNAs

155 ation in the dimerization interface inhibits editing in an RNA substrate-dependent manner for both AD

156 t implications for the application of genome editing in both basic research and clinical practice.

157 ted miRNA-binding site by CRISPR-Cas9 genome editing in C. elegans We developed a multiplexed negativ

158 icism to levels that make single-step embryo editing in cattle commercially feasible.

159 increased the speed and precision of genetic editing in cells and animals.

160 escribe a platform for efficient Cas12a gene editing in Drosophila We show that Cas12a from Lachnospi

161 lopment and the first to interrogate U-indel editing in EMF and MCF life cycle stages.

162 d variants mediated indel formation and base editing in human cells and enabled A*T-to-G*C base editi

163 of human association analysis, CRISPR genome editing in mice, animal behavioural analysis and cell cu

164 rmore, our highly efficient CRISPR/Cas9 gene editing in primordial germ cells represents a substantia

165 ere we establish CRISPR/Cas9-mediated genome editing in S. rosetta by engineering a selectable marker

166 enario where levels of mature RNA species or editing in the single T. cruzi mitochondrion are linked

167 incorporated zebularine also did not disrupt editing in vitro, suggesting that PPR65 cannot bind modi

168 ogy for memory CD8 T cells to undertake gene editing in vivo, for the first time, to our knowledge.

169 idaminococcus spec., can mediate robust gene editing in vivo.

170 ome editing reagents can mitigate off-target edits in plants.

171 RISPR base editors that make precise genomic edits in rice protoplasts while minimizing untargeted mu

172 In vitro genome editing indicated that the SRF enhancer CArG box regulat

173 Here, using a combination of isotope-edited infrared spectroscopy and molecular dynamics simu

174 Within the testis, RNA editing is catalyzed by ADARB1 and is regulated in a cel

175 Genome editing is-or will soon be-in the clinic for several dis

176 Impaired ADAR2 editing leads to early-onset epilepsy and premature deat

177 ells that loss of RAZUL by CRISPR-based gene editing leads to loss of E6AP at proteasomes.

178 gative selection screening approach in which edited loci are deep sequenced, and candidate sites are

179 papaya chloroplast genome, there are 46 RNA editing loci with an average RNA editing efficiency of 6

180 egranulation profiles of both edited and non-edited mast cells, offering a consistent internal contro

181 PTEN silencing with CRISPR/dCas9 epigenetic editing may provide a new option for promoting axon rege

182 Due to breakthroughs in RNAi and genome editing methods in the past decade, it is now easier tha

183 mapping-by-sequencing and CRISPR/Cas9 genome editing methods, we isolated EXCESSIVE NUMBER OF FLORAL

184 We find that patient-derived and genome edited MINPP1(-/-) induced stem cells exhibit an ineffic

185 h the ability to precisely target by genomic editing most areas of the genome, is producing important

186 e first analysis of the mechanisms governing edited mRNA levels during T. brucei development and the

187 ding screens that harness CRISPR/Cas9 genome editing, natural genetic variation, proteomics, and tran

188 We found evidence that alternatively 5'-edited ND7 5' and CR3 transcripts are present in the tra

189 was measured with the (1) H-observed/(13) C-edited NMR method.

190 apeutics and the improvement of precise gene editing now pave the way to applications such as cardiac

191 Gene editing nuclease represented by Cas9 efficiently generat

192 AR substrates and the features governing RNA editing observed in our study will assist in the rationa

193 A single therapeutic base edit of the BCL11A enhancer prevented sickling and ameli

194 g in human cells and enabled A*T-to-G*C base editing of a sickle cell anemia mutation using a previou

195 RNA libraries, which can be used for genome editing of coding and non-coding genomic regions effecti

196 pression in mouse cells and CRISPR/Cas9 base editing of endogenous AGS loci revealed causal roles of

197 Here, we describe CRISPR/Cas9-based editing of exon 1 of the HVT079 and HVT096 genes from th

198 esults demonstrate the potential of RNP base editing of human HSPCs as a feasible alternative to nucl

199 Congruently, gene editing of LAYN in human CD8+ T cells reduced direct tum

200 ion processes has enabled precise, multiplex editing of microbial genomes and the construction of bil

201 Precise gene editing of mitochondrial DNA (mtDNA) is essential for th

202 A-to-I editing of RNA is a widespread posttranscriptional proce

203 few approaches have been developed for gene editing of these cell types, likely owing to their sensi

204 stence of fetal hemoglobin (HPFH) mutations, editing of transcriptional HbF repressors or their bindi

205 injection) to detect off-target mutations by editing one blastomere of two-cell mouse embryos using e

206 ize single genes but ultimately to massively edit or write from scratch entire genomes.

207 added to cells to create targeted deletions, edits, or additions to the chromosome.

208 typic penetrance due to the mosaic nature of editing outcomes after double strand break (DSB) repair.

209 y efficiencies, and suppression of imprecise editing outcomes at the on-target site as key design par

210 hes can be used to forecast CRISPR/Cas9 gene editing outcomes in Xenopus tropicalis, Xenopus laevis,

211 how that CGBE1 can efficiently induce C-to-G edits, particularly in AT-rich sequence contexts in huma

212 In this study, we analyzed the editing patterns of three putative dual-coding genes, ri

213 In a population of these CRISPR-Cas9 edited plants (n = 780) that was phenotyped for tassel f

214 The creation of edited plants through tissue culture is often inefficien

215 We briefly introduce three epigenome-editing platforms: zinc-finger proteins, transcriptional

216 or genotoxicity was associated with the gene editing process, paving the way for an alternative, yet

217 alternative to the most commonly used genome editing protein Streptococcus pyogenes Cas9 (SpyCas9), w

218 We were able to achieve an editing rate of > 99% for multiple genes that functional

219 to produce an inconsistent range of genomic editing rates between 0.03-3%.

220 oligodeoxynucleotides mediated precise gene editing rates, and effectively reduces on-target inserti

221 An alternative is to introduce genome editing reagents and a homologous recombination (HR) don

222 Developmental regulators and gene-editing reagents are delivered to somatic cells of whole

223 ng computational algorithms to design genome editing reagents can mitigate off-target edits in plants

224 d the safety and efficacy concerns of genome editing remain.

225 Efficient base editing requires cellular exposure to levels of base edi

226 However, CRISPR-mediated gene editing revealed that PKA and AMPK are not required for

227 using the recently proposed FESTA (Fluorine-Edited Selective TOCSY Acquisition) methodology.

228 mn) space algorithm to find the minimum cost edit sequence between strings of length m and n, respect

229 CRISPR-mediated gene editing shows promise to cure genetic pathologies, altho

230 uption of GAL5.1 in mice using CRISPR genome editing significantly reduced GAL expression in the amyg

231 esponse, epithelial-mesenchymal differential editing sites are enriched in genes involved in immune a

232 NA-seq) enables global identification of RNA-editing sites in biological systems and disease.

233 salient step in many studies is to identify editing sites that statistically associate with treatmen

234 1) in DLD-1 CRC cells using CRISPR/Cas9 gene editing; some cells were transfected with plasmids that

235 expanded their targeting scope and improved editing specificity.

236 rget analysis was applied to investigate the edited strains after curing of the base editor plasmid.

237 Here we show that a CRISPR/Cas9-based genome editing strategy allows the precise correction of WAS mu

238 analyze differences between ADAR1 and ADAR2 editing substrates.

239 nosoma brucei possesses a highly complex RNA editing system that uses guide RNAs to direct the insert

240 leic-acid editing ability of the dCas13a RNA-editing system.

241 PR/dCas9-based enhancer-targeting epigenetic editing systems, enCRISPRa and enCRISPRi, for efficient

242 hat clinical application of CRISPR-Cas9 gene-edited T cells is generally safe and feasible.

243 exploratory objectives included tracking of edited T cells.

244 domains: vaccine research, viral transcript editing, T-cell effector response targeting including ch

245 -membrane-coating nanotechnology and genetic editing technique offers a safe and robust strategy in a

246 Gene-editing techniques are currently revolutionizing biology

247 Herein, we exploited spectral editing techniques to identify and quantify the lipid mo

248 SORT is compatible with multiple gene editing techniques, including mRNA, Cas9 mRNA/single gui

249 aracterizing new varieties generated by gene-editing techniques.

250 S. viridis research, highly efficient genome editing technologies are needed to create genetic variat

251 uman beta-cell lines, and advances in genome-editing technologies coupled with improved protocols dif

252 emonstrations, and recent advances in genome editing technologies may enable the use of reporters in

253 cal step in the future translation of genome editing technologies.

254 The advent of genome editing technology provides new opportunities to correct

255 d BMI1 as the direct target of AR using gene-editing technology.

256 lthough it is technically possible to genome-edit the chicken, its long generation time (6 months to

257 Finally, we discuss current tools used to edit the composition of the glycocalyx and the future ch

258 ADR-1 promotes SLO-2 function not by editing the transcripts of slo-2 but those of scyl-1, wh

259 Using CRISPR/Cas9 genome editing, the enhancer cluster or parts thereof, Nppb and

260 osing organisms, designing robots capable of editing their own structure to more efficiently perform

261 d nanoparticles were designed to selectively edit therapeutically relevant cell types including epith

262 development of efficient, combinatorial gene-editing therapeutics.

263 important in the development of directed RNA editing therapeutics.

264 been subjected to CRISPR/Cas9-mediated gene editing, there is no evidence of success in genetic alte

265 With recent advances in genome editing, this type of genetic mutation can be precisely

266 ecT increases the efficiency of single-locus editing to as high as 50% and improves multiplex editing

267 ene-edited cell populations and expands gene editing to chromosomal tracts previously not possible to

268 Genome editing to correct a defective beta-globin gene or induc

269 This work establishes that precise gene editing to correct multiple distinct gene variants could

270 Using genome editing to delete candidate REs, we showed that a strong

271 We then use genome editing to disrupt the coding sequence of a S. rosetta C

272 Employing CRISPR/Cas9 gene editing to disrupt the Pdgfra gene in two different muri

273 Using CRISPR/Cas9 editing to endogenously tag receptors with fluorescent p

274 Here we use genome editing to engineer a general platform to improve the sa

275 We previously used gene editing to introduce a dual epitope tag into the endogen

276 cobalamin metabolic enzymes, we used genome editing to study the loss of mmachc function and to deve

277 ajectories, and allow users to perform basic edits to DNA and RNA designs.

278 describe an optimized Cas9-AAV6-based genome editing tool platform for site-specific mutagenesis and

279 Thus, REPAIRx markedly expands the RNA editing toolkit and illustrates a novel strategy for bas

280 Here, we have developed multiplex genome editing toolkits for citrus including PEG-mediated proto

281 We have developed multiplex genome editing toolkits for citrus that significantly improve c

282 The development of new CRISPR-Cas genome editing tools continues to drive major advances in the l

283 the applicability of CRISPR-associated gene editing tools in vitro and in vivo.

284 ceosome, with potential applications as gene-editing tools.

285 tial for application as modulators of genome editing tools.

286 an steers a tapasin loop involved in peptide editing toward the binding groove.

287 can be repurposed to bind and isolate A-to-I edited transcripts from cellular RNA.

288 Genome editing typically involves recombination between donor n

289 Genome editing using programmable nucleases is revolutionizing

290 Expressed from the P gene through mRNA editing, W shares a common N-terminus with P and V but h

291 combining quantitative genetics with genome editing, we show how multiple SVs that changed gene dosa

292 With CRISPR-Cas9 genome editing, we validated Less Shattering1 (SvLes1) as a gen

293 levels of successful heritable plant genome editing were addressed using simple case studies in Arab

294 Ajugoideae, and are generally devoid of RNA editing, whereas moderately diverged genes accelerated l

295 ominant Best disease iPSC-RPE models to gene editing, which produced premature stop codons specifical

296 swine primary fetal fibroblasts, which were edited with TALENs and single-strand oligonucleotide to

297 Cas12i could potentially be used for genome editing with high specificity.

298 ation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of

299 ) reveal a novel mechanism of stereochemical editing within peptidoglycan transpeptidation, (c) asses

300 CRISPR-Cas9-edited zebrafish were used as an in vivo model to assess