ライフサイエンスコーパス: effector domain

1 ization of the influenza A virus NS1 protein effector domain.

2 ire length of the molecule to the N-terminal effector domain.

3 as exerted by the PAS domain proximal to the effector domain.

4 and Ptr-H16, fusing the P. furiosus ORF1231 effector domain.

5 -associated death domain (FADD) at the death effector domain.

6 I and HIP1 was predicted to resemble a death-effector domain.

7 ot for the growth-suppressive effects of the effector domain.

8 ts is suppressed by a second mutation in the effector domain.

9 ough Fas/CD95 and DR5 were also in the death effector domain.

10 site to the N-terminal end of the FADD death effector domain.

11 osphorylation domain inhibits the C-terminal effector domain.

12 ical for GTPase activity and in its putative effector domain.

13 decoupling of the signal pathway within the effector domain.

14 oes not interact with its NH2-terminal death effector domain.

15 eraction domains: a death domain and a death effector domain.

16 K-Ras in a GTP-dependent manner via the Ras effector domain.

17 main inhibits methylesterase activity of the effector domain.

18 g and transcription activation in the distal effector domain.

19 binding properties of the carboxyl-terminal effector domain.

20 and stimulation of enzymatic activity of the effector domain.

21 nslocation of the carotenoid deeper into the effector domain.

22 ed both an intact NS1 RNA-binding domain and effector domain.

23 oP-DNA interactions exclusively involved the effector domain.

24 arotenoid has translocated entirely into the effector domain.

25 ted catalytically inactive dCas9 fused to an effector domain.

26 ealing allosteric control of the CNBD by the effector domain.

27 N-terminal receiver domain and a C-terminal effector domain.

28 site recognition through their Arg-Ser-rich effector domains.

29 encode toxins with different arrangements of effector domains.

30 odules to propagate environmental signals to effector domains.

31 specifically at leucine residues between the effector domains.

32 rocessing and intracellular release of toxin-effector domains.

33 hyl-lysine recognition by two closely spaced effector domains.

34 l changes brought about by NTP hydrolysis to effector domains.

35 control the output activities of C-terminal effector domains.

36 to execute their as yet unknown function as effector domains.

37 mploy different mechanisms to regulate their effector domains.

38 teins, PAS domains bind ligands and modulate effector domains.

39 ough the opposing activities of its terminal effector domains.

40 nated heme and regulates the activity of its effector domain, a C-terminal histidine kinase, in respo

41 Despite all effector domains acting on cytoskeleton assembly, the AC

42 interactions that either inhibit or activate effector domain activities.

43 Recent studies have indicated that the Fc effector domain also displays considerable heterogeneity

44 S protein and a peptide corresponding to the effector domain (an unstructured region that contains 13

45 lls (IECs) and uses a MARTX toxin with three effector domains-an actin cross-linking domain (ACD), a

46 ly, are composed of an N-terminal Nudix-like effector domain and a C-terminal DNA-binding HTH-like do

47 y modular, consisting of a sensor domain, an effector domain and a keto-carotenoid.

48 nse regulators stimulates DNA binding by the effector domain and how dimerization and domain orientat

49 1844-2114 (PaTox(P)), upstream of these two effector domains and characterized by three conserved am

50 thermore, we detected numerous new conserved effector domains and discovered new domain combinations,

51 itions C-terminal specificity and N-terminal effector domains and facilitates stable binding to adjac

52 in filaments to drive oligomerization of TIR effector domains and rapid NAD(+) cleavage.

53 x family comprising an N-terminal Nudix-like effector domain, and a C-terminal DNA-binding winged hel

54 e expression, a function requiring an intact effector domain, and via altering RpaA phosphorylation,

55 n modules that includes death domains, death effector domains, and Caspase activation and recruitment

56 y effector domains may depend on which other effector domain are co-delivered.

57 The death domain and death effector domain are two common motifs that mediate prote

58 nd inactivate RhoA, respectively, when other effector domains are absent, with toxin autoprocessing r

59 tions into the molecular mechanisms of these effector domains are providing insight into how the func

60 les indicates that Mgm1p's GTPase and GTPase effector domains are required for its ability to promote

61 that artificial DNA-binding proteins lacking effector domains are useful tools for studying and modul

62 t domain), DD (death domain), and DED (death effector domain), are believed to exert their effects so

63 ated that these miniproteins bind to the Ras effector domain as dimers, and high-resolution crystal s

64 ns could be integrated and propagated to the effector domain as torques.

65 previously characterised response regulator effector domains, as it is shorter than any characterise

66 ath receptors such as Fas, whereas the death effector domain binds to procaspase 8.

67 apters such as TRADD, whereas the FADD death effector domain binds to procaspase 8.

68 Kinetic measurements suggest the effector domain binds to several PIP(2).

69 important for HIPPI binding, is not a death-effector domain but is a partially opened coiled coil.

70 nstitutively active form of RacE require its effector domain, but not PIP3.

71 ylation of a tyrosine residue located in its effector domain by an Eph receptor kinase.

72 s (Rab8, 10, 13 and 15) and that some of the effector domains can bind two Rab proteins via separate

73 lar proteins consist of death domains, death effector domains, caspase recruitment domains, and pyrin

74 allosteric communication between sensor and effector domains, characterization of all relevant signa

75 rylation domain and a C-terminal DNA binding effector domain connected by a flexible interdomain link

76 We demonstrate that death effector domain containing DNA binding protein (DEDD), a

77 DD), a highly conserved and ubiquitous death effector domain containing protein, exists predominantly

78 ase-3, caspase-cleaved cytokeratin-18, death-effector-domain containing DNA-binding protein and ubiqu

79 In this study, we reveal that Death Effector Domain-containing DNA-binding protein (DEDD), w

80 PEA-15 is a death effector domain-containing phosphoprotein that binds ERK

81 Here we demonstrate that a small death effector domain-containing protein called PEA-15 binds R

82 Interestingly, death effector domain-containing proteins such as MC159, E8, K

83 V. vulnificus mutants with varying effector domain content were inoculated intragastrically

84 culate that low thermostability of the MARTX effector domains correlates with that of many other memb

85 Rather, two conserved transcriptional effector domains (CR2 and CR3) of AFX are required for f

86 roteins required 2 conserved transcriptional effector domains (CR2 and CR3), each of which alone was

87 Fusing TALEs with effector domains creates synthetic transcription factors

88 PEA-15 is composed of an N-terminal death effector domain (DED) and a C-terminal tail of irregular

89 solved in solution, revealing that the death effector domain (DED) and death domain (DD) are aligned

90 ave reported that E6 binds to the FADD death effector domain (DED) at a novel E6 binding domain.

91 of apoptosis proteins (IAP); however, death effector domain (DED) caspases of the extrinsic pathway

92 ers recruitment of FADD, which via its death effector domain (DED) engages the DEDs of procaspase 8 a

93 ilaments in turn nucleate procaspase-8 death effector domain (DED) filaments in vitro and in vivo.

94 The death effector domain (DED) occurs in proteins that regulate p

95 g the death domain (DD) subfamily, the death effector domain (DED) subfamily, the caspase recruitment

96 One of these domains is the death effector domain (DED) that is predominantly found in com

97 2(PYD) with the hydrophobic patches of death effector domain (DED)-containing proteins and confirm th

98 iated by conserved domains such as the death effector domain (DED).

99 sical protein complex with Src via its death effector domains (DED) and maintains the complex in a de

100 Proteins with death effector domains (DED) are key signal transducers involv

101 ns (CARD); (3) Death Domains (DD); (4) Death Effector Domains (DED); (5) BIR domains of Inhibitor of

102 nsisting of only the N-terminal tandem death effector domains (DEDs) (N protein) was dramatically dec

103 Homophilic interactions of death effector domains (DEDs) are crucial for the signaling pa

104 Death effector domains (DEDs) are protein interaction modules

105 The prodomains death effector domains (DEDs) of caspase 8 were sufficient for

106 ains (DDs) of Fas and FADD and between death effector domains (DEDs) of FADD and caspase-8/10.

107 Unexpectedly, the two tandem death effector domains (DEDs) of MC159 rigidly associate with

108 LIP and, as such, possesses two tandem death effector domains (DEDs).

109 this family is the presence of tandem death-effector domains (DEDs).

110 utoprocessing repeats-in-toxin (MARTX) toxin-effector domain DUF5(Vv) from Vibrio vulnificus to be a

111 ed that by combining zinc fingers with other effector domains, e.g., from nucleases or integrases, to

112 g NCAM antibody and a peptide comprising the effector domain (ED) of myristoylated alanine-rich C kin

113 vestigated the hypothesis that the polybasic effector domain (ED) of the abundant intracellular subst

114 s, we demonstrate that cell permeable MARCKS effector domain (ED) peptides potently target all GBM mo

115 nsists of an RNA-binding domain (RBD) and an effector domain (ED) separated by a flexible linker regi

116 domains, an RNA-binding domain (RBD) and an effector domain (ED) separated by a linker region (LR),

117 MARCKS binds with its functionally essential effector domain (ED) to polysialic acid.

118 RNA (dsRNA)-binding domain and a C-terminal effector domain (ED).

119 vity of their DNA-binding domains (DBDs) and effector domains (EDs) enabling the coordination of gene

120 hybrid proteins developed to direct various effector domains (EDs) of choice to predetermined DNA se

121 sion of FADD that lacks the N-terminal death effector domain (FADD(DN)) increases the sensitivity of

122 c triad is essential for function of the RID effector domain family shared by MARTX toxins produced b

123 onstrate that the MeV H stalk represents the effector domain for MeV F triggering.

124 domain is exploited in a screen of other Lrp effector domains for activation capability by constructi

125 hytochromes to interact with various protein effector domains for biological modulation of diverse ph

126 structures of the RNA binding domain and the effector domain from non-H5N1 strains, the RNA binding d

127 sidues in the linker between the RBD and the effector domain from the other chain.

128 selected targets, to examine the activity of effector domains from natural splicing factors and to mo

129 the RID MLD with the MLDs from two different effector domains from the Vibrio vulnificus MARTX toxin

130 ere, we report the crystal structures of the effector domains from two oomycete RXLR proteins, Phytop

131 The Plasmodium effector domains functionally complement the loss of the

132 tudies of transcription activator-like (TAL) effector domains fused to nucleases (TALENs) demonstrate

133 oters, multiplexed guide RNA expression, and effector domain fusions to SpCas9.

134 dynamin 1's catalytic G domain to its GTPase effector domain (GED) at 2.2 A.

135 a C-terminal sequence related to the GTPase effector domain (GED) in dynamin.

136 Dynamin's GTPase effector domain (GED) is required for this stimulation,

137 rizes and forms the tube surface, the GTPase effector domain (GED) mediates self-assembly, and the pa

138 n of the GTPase domain and two in the GTPase effector domain (GED), dynamin's putative GAP, fully res

139 both are indirectly influenced by the GTPase effector domain (GED).

140 in cross-linking reaction performed by these effector domains has been significantly characterized, t

141 bined with the potential of fusing them with effector domains has led to the technology of engineerin

142 ructures of numerous isolated regulatory and effector domains have been determined.

143 r, the currently available structures of NLR effector domains have not yet revealed the mechanism of

144 depends on the coupling of CO binding at an effector domain heme with the allosteric repositioning o

145 bles a similar surface involved in the death effector domain homotypic interactions.

146 ne repression required the action of several effector domains; however, KRAB-dCas9 did not contribute

147 ins and thereby positioning LMP-1's critical effector domains (i.e. the carboxy-terminus).

148 Expression of the NORE1A effector domain in A549 lung adenocarcinoma cells result

149 oduce hybrid RtxA::Bla toxins that carry one effector domain in addition to Bla were found to more ef

150 ient energy transfer when bound to YPET-GGA3 effector domain in intact cells.

151 in, whereas the CARD domain functions as the effector domain in the caspase-1 activation pathway.

152 dent mechanism to control a diverse array of effector domains in biological and engineered proteins.

153 monstrate that even the structurally similar effector domains in rabenosyn-5 can achieve highly selec

154 to stabilize the active conformation of the effector domain increased the agonist potency in stabili

155 struct access to the functional sites of the effector domains, indicating a regulatory mechanism base

156 mino-terminus to LMP-1's function: (i) as an effector domain, interacting with cellular proteins, or

157 (iii) a NF-kappaB Essential Modifier-binding effector domain interfering with NF-kappaB activation.

158 eine protease domain for the delivery of the effector domain into host cytosol.

159 ranslocate multiple functionally independent effector domains into a target eukaryotic cell.

160 l for virulence and that the ExoY-like MARTX effector domain is a catalytically active adenylate cycl

161 ional activation through the C-terminal Ptr2 effector domain is exploited in a screen of other Lrp ef

162 as in an unprecedented mode in which the Ras effector domain is remodeled to expose an extended pocke

163 One well-characterized effector domain is the C-terminal actin cross-linking do

164 known and the translocation mechanism of the effector domains is poorly understood.

165 equirement for alpha-helical transcriptional effector domains is similar to the oncogenic contributio

166 onding to a basic region in GAP43 and MARCKS effector domain-like regions in other proteins (e.g. Mac

167 in vitro study by Gay et al., suggests that effector domain MARCKS peptides could play a significant

168 he ability of a peptide corresponding to its effector domain, MARCKS(151-175), to sequester PIP2 in m

169 Instead, modulation of cell function by effector domains may depend on which other effector doma

170 that the Makes Caterpillar Floppy-like MARTX effector domain (MCFVv) is an autoproteolytic cysteine p

171 the serine/threonine kinase Raf-1 or the Ras effector domain mutant 12V/35S, which retains binding to

172 subunit of PI3-K, p110alpha-CAAX, or the Ras effector domain mutant 12V/40C, which retains binding to

173 Moreover, transfection with effector domain mutant constructs of active H-Ras showed

174 A weakly transforming effector domain mutant of activated Wrch-1 that inhibits

175 An effector domain mutant of HRas, HRasN17G37, selectively

176 Expression of a Rac1 effector domain mutant that does not bind Pak rescues gr

177 We used effector domain mutants (EDMs) to explore the relationsh

178 We also generated cytosolic GTP-bound Ras effector domain mutants (EDMs), each of which reduced th

179 R-Ras effector domain mutants and pharmacological inhibition s

180 In addition, expression of effector domain mutants of Rac1-V12 that exhibit reduced

181 or signals as revealed by the examination of effector domain mutants of RhoA.

182 Using effector domain mutants of RhoG we found that its abilit

183 Analysis of RhoB effector domain mutants pointed to a role for PRK, a Rho

184 -dependent signaling, whereas the FADD death effector domain mutants rescued only TNF signaling.

185 DNA binding by the effector domain (NarLC) of the response regulator, NarL,

186 ties of AVR3a are mediated by its C-terminal effector domain, not its RxLR leader.

187 ionally modular, consisting of an N-terminal effector domain (NTD) and a C-terminal regulatory domain

188 minal regulatory domain (CTD), an N-terminal effector domain (NTD) and a ketocarotenoid; the chromoph

189 f the chlorinated aromatic compound with the effector domain of CprK triggers binding of CprK to an u

190 The FADD-MBD4 interaction involved the death effector domain of FADD and a region of MBD4 adjacent to

191 ntly of the death effector domain, the death effector domain of FADD comes into direct contact with b

192 hat in contrast to current models, the death effector domain of FADD is involved in interaction with

193 previously identified mutations in the death effector domain of FADD that prevented binding to Fas/CD

194 onstrate that E6 binds directly to the death effector domain of Fas-associated death domain (FADD), w

195 ntaining the linked N-terminus and the basic effector domain of GAP-43.

196 entified in Drosophila Tube DD and the death effector domain of hamster PEA-15, two physiologically u

197 ion between PSA and a peptide comprising the effector domain of MARCKS (MARCKS-ED).

198 layer confirming the notion that PSA and the effector domain of MARCKS interact at and/or within the

199 Our working hypothesis is that the effector domain of MARCKS reversibly sequesters a signif

200 In the presence of a peptide comprising the effector domain of MARCKS the EPR spectrum broadens, but

201 The LPS binding site within the effector domain of MARCKS was narrowed down to a heptape

202 The basic effector domain of myristoylated alanine-rich C kinase s

203 rresponding to the basic (+13), unstructured effector domain of myristoylated alanine-rich C kinase s

204 The effector domain of myristoylated alanine-rich C-kinase s

205 nctionally, we show that both the N-terminal effector domain of NLRC5 and its C-terminal leucine-rich

206 g activity and is mediated by the C-terminal effector domain of NS1.

207 ts switch I region that is equivalent to the effector domain of other Ras-like small GTPases.

208 AF6, which directly interacts with the death effector domain of pro-caspase 8, and the N-terminal RIN

209 cture determination by NMR of the C-terminal effector domain of PrrA, PrrAC.

210 In contrast, the effector domain of Ptr1, the M. jannaschii Ptr2 paralogu

211 re effective activators: Ptr-H10, fusing the effector domain of Pyrococcus furiosus LrpA, and Ptr-H16

212 ually binds Rab10 directly through a defined effector domain of Rab10 and the middle domain of Dyn2.

213 arget new cellular proteins by replacing the effector domain of rapamycin with a combinatorial librar

214 We also find that the effector domain of Rheb is required for the mTORC1 activ

215 In addition, the effector domain of Ryh1 is important for its physical in

216 he Spt5 Nus-G N-terminal (NGN) domain is the effector domain of the complex that both mediates the in

217 ifically, experiments suggest that the basic effector domain of the myristoylated alanine-rich C kina

218 The effector domain of the myristoylated alanine-rich C-kina

219 ptide comprising the phosphorylation site or effector domain of the protein acts as a powerful inhibi

220 udomonas syringae pv tabaci that deliver the effector domain of the Xcv AvrBs2 protein via the TTSS o

221 The presence of two death effector domains of c-FLIP and S-nitrosylation of its ca

222 Effector domains of MARTX toxins cross the cytoplasmic m

223 Among the three effector domains of MARTX(Vc) is the Rho inactivation do

224 Isolated RNA-binding and effector domains of NS1A both exist as homodimers in sol

225 Unlike other effector domains of the multifunctional toxin that targe

226 sion and activation are mediated by separate effector domains of this protein.

227 examined the thermodynamic stability of the effector domains of V. cholerae and A. hydrophila MARTX

228 Overall, the binding of the PBD46 effector domain on the dynamics of methyl-bearing side c

229 LOV domains are associated with a variable effector domain or a separate protein signaling partner

230 Mutations in either the death effector domain or C-terminal tail of PEA-15 that block

231 yristoylated alanine-rich C kinase substrate effector domain) or elevated concentrations of Sec17p, w

232 Second, removing basic residues from the effector domain peptide reduces the binding energy by an

233 vesicles with the same high affinity as the effector domain peptide.

234 Rac effector domain point substitutions (A27K, G30S, D38A, Y

235 Based on the type of effector domain present, we classified six as NarL type,

236 ccompanied by a conformational change of the effector domain, presenting a block in activation subseq

237 Theoretical calculations show the effector domain produces a local positive potential, eve

238 The scaffolding protein PEA-15 is a death effector domain protein that directly interacts with ERK

239 prisingly, these mutations were in the death effector domain rather than the death domain.

240 ble cysteine protease domain to the adjacent effector domain reduces its thermodynamic stability appr

241 The effector domain region is essential for lethal intestina

242 The effector domain region was required for V. vulnificus to

243 consisting of the GTPase, middle, and GTPase effector domain regions.

244 Our results indicate that effector domain regulation by either N terminus requires

245 ing substantially different contributions to effector domain regulation in individual members of the

246 coiled-coil C-helix interface, and that this effector domain reorientation stabilizes the active posi

247 gh these proteins share the majority of core effector domain residues with Ras, recent studies sugges

248 The effector domains responsible for these activities are he

249 r and intracellular Par-4 acting through its effector domain SAC.

250 F) and SMODS-associated and fused to various effector domains (SAVED) are key components of cyclic ol

251 as two domains, an RNA-binding domain and an effector domain separated by a linker.

252 -stranded RNA (dsRNA) binding domain and the effector domain, separated through a linker-is an antago

253 ts modest structural changes, while the H5N1 effector domain shows significant alteration, particular

254 and found that biotype 3 MARTX toxin has an effector domain structure distinct from that of either b

255 , a response regulator, and in some cases an effector domain such as an adenylyl or guanylyl cyclase,

256 at the inflammasome through its tandem death effector domain (tDED), which is regulated by the tDED-c

257 oligonucleotide sensor stem-loop and an RNAi effector domain that is designed to undergo a structural

258 tudy, we have thoroughly characterized a Rab effector domain that is present in proteins of the Mical

259 urrent small GTPase sensors rely on specific effector domains that are available for only a small num

260 ulti-domain proteins with varying N-terminal effector domains that are responsible for regulating dow

261 ns are composite toxins comprised of arrayed effector domains that carry out distinct functions insid

262 ulated switches to control the activities of effector domains that elicit output responses.

263 of two F2F3 molecules wraps around two NS1A effector domains that interact with each other head-to-h

264 king the DNA-binding protein to a variety of effector domains that mediate transcriptional activation

265 ependent growth assays, we mapped the NORE1A effector domain (the minimal region of the protein respo

266 of FADD functions independently of the death effector domain, the death effector domain of FADD comes

267 ress has been made characterizing individual effector domains, the role of paired domains and how the

268 we identify a novel class of methylated H3K4 effector domains--the PHD domains of the ING (for inhibi

269 and are transmitted to the spatially distant effector domain, thereby regulating its histidine kinase

270 pite sharing <20% sequence identity in their effector domains, they display a conserved core alpha-he

271 ated K-Ras in a GTP-dependent manner via the effector domain, thus exhibiting the basic properties of

272 sed to disrupt this interaction, causing the effector domain to be released and free to bind DNA.

273 These studies specifically link the MCFVv effector domain to induction of the intrinsic apoptosis

274 itored to establish the contribution of each effector domain to overall virulence.

275 g enzymes evolved by the fusion of ancillary effector domains to an ancestral catalytic module involv

276 A-binding domain was engineered and fused to effector domains to either repress (G9a, Suvdel76, SKD)

277 (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.

278 Vibrio cholerae MARTXVc toxin delivers three effector domains to eukaryotic cells.

279 mice, but the contribution of each of the 5 effector domains to infection has not been investigated.

280 his bacterium through delivery of up to five effector domains to the host cells.

281 pike, which is further involved in attaching effector domains to the spike.

282 Using a unique combination of targeting and effector domains, TT30 controls cell surface CAP activat

283 Their N-terminal effector domains (typically a pyrin or caspase activatio

284 complex is dependent on the variant of death effector domain (vDED) in Bap31 and is required for the

285 No single MARTX effector domain was necessary for bacterial disseminatio

286 gocytosis by J774 macrophages, but no single effector domain was required.

287 e requirement for 2 distinct transcriptional effector domains was also displayed by VP16, which requi

288 an forkhead family member that contains both effector domains, was also capable of transforming hemat

289 ned action of myristoylation and a polybasic effector domain, which binds phospholipids and/or F-acti

290 unusual in that it lacks a conserved GTPase Effector Domain, which is typically required for functio

291 y unfolded protein with a conserved cationic effector domain, which mediates the cross-talk between s

292 ore, mutagenesis of Blimp-1 reveals multiple effector domains, which regulate distinct genes.

293 lator composed of a regulatory domain and an effector domain with enzymatic activity.

294 Here, we show that fusion of dCas9 to effector domains with distinct regulatory functions enab

295 s that are found associated with one or more effector domains with diverse functions.

296 Human BRPF1 contains multiple effector domains with known roles in gene transcription,

297 ylation receiver (REC) domain flanked by two effector domains with putative enzymatic activities (ser

298 n flanked by putative kinase and phosphatase effector domains with similarity to partner-switching pr

299 IMCyp gene encodes a protein fusion of TRIM5 effector domains with the capsid-binding ability of a re

300 nsights into the mechanism by which distinct effector domains within a protein cooperatively modulate