ライフサイエンスコーパス: egg extract

1 immobilized in agarose prior to addition of egg extract.

2 cell-free system based on interphase Xenopus egg extract.

3 embly of anastral spindles in Xenopus laevis egg extract.

4 is Ran- and importin alpha-dependent in the egg extract.

5 tabilization and Axin degradation in Xenopus egg extract.

6 ction partner of SUMOylated PARP1 in Xenopus egg extract.

7 ned ATR-activating DNA structures in Xenopus egg extracts.

8 using crude and fractionated Xenopus laevis egg extracts.

9 spindle assembly around DNA-coated beads in egg extracts.

10 se spindles in Haemanthus endosperm and frog egg extracts.

11 totic chromosomes in human cells and Xenopus egg extracts.

12 of existing microtubules in meiotic Xenopus egg extracts.

13 ffer or during chromatin assembly in Xenopus egg extracts.

14 d it to replication-competent Xenopus laevis egg extracts.

15 etaphase spindle assembled in Xenopus laevis egg extracts.

16 S50/T64/T68/T79/S114/S165) by CDK in Xenopus egg extracts.

17 n-coated beads after introduction of Xenopus egg extracts.

18 using Ran-mediated asters in meiotic Xenopus egg extracts.

19 ation protein complexes from Xenopus meiotic egg extracts.

20 th strand-specific DNA roadblocks in Xenopus egg extracts.

21 es DNA damage in oxidative stress in Xenopus egg extracts.

22 tion that occurs at the MBT is reproduced in egg extracts.

23 dent kinase (Cdk) activity in Xenopus laevis egg extracts.

24 ld increase of Lis1 concentration in Xenopus egg extracts.

25 res of meiotic spindles assembled in Xenopus egg extracts.

26 essential for mitotic SUMOylation in Xenopus egg extracts.

27 skewer metaphase spindles in Xenopus laevis egg extracts.

28 ules, to support spindle assembly in Xenopus egg extracts.

29 eslin that associates with TopBP1 in Xenopus egg extracts.

30 ontaining Cdc7, Drf1, and Claspin in Xenopus egg extracts.

31 the ATR-activating protein TopBP1 in Xenopus egg extracts.

32 necessary for spindle bipolarity in Xenopus egg extracts.

33 and nonspindle assemblies, in Xenopus laevis egg extracts.

34 pulation of preassembled spindles in Xenopus egg extracts.

35 determine their repair mechanism in Xenopus egg extracts.

36 d an in vitro import system based on Xenopus egg extracts.

37 depends upon ATM in human cells and Xenopus egg extracts.

38 for M phase entry and maintenance in Xenopus egg extracts.

39 fusion and nuclear pore assembly in Xenopus egg extracts.

40 regulate nuclear and spindle size in Xenopus egg extracts.

41 tebrate meiotic spindle assembled in Xenopus egg extracts.

42 for a model mitotic CDK substrate in Xenopus egg extracts.

43 alcium-induced exit from metaphase arrest in egg extracts.

44 he model were tested and verified in Xenopus egg extracts.

45 chondrial enzyme, from HeLa cell and Xenopus egg extracts.

46 he Rad9-Hus1-Rad1 (9-1-1) complex in Xenopus egg extracts.

47 ATR-dependent checkpoint pathway in Xenopus egg extracts.

48 extracts employing Xenopus laevis oocytes or egg extracts.

49 fective inhibitor of checkpoint signaling in egg extracts.

50 nd ATR in a DNA damage checkpoint in Xenopus egg extracts.

51 ight affect DNA replication, we used Xenopus egg extracts.

52 ounterpart when incubated in Xenopus M phase egg extracts.

53 MAPK phosphorylates Mps1 at S844 in Xenopus egg extracts.

54 unction, we examined its behavior in Xenopus egg extracts.

55 induce Op18 hyperphosphorylation in Xenopus egg extracts.

56 d physiological spindle assembly activity in egg extracts.

57 to stalled DNA replication forks in Xenopus egg extracts.

58 yte maturation and during mitosis in Xenopus egg extracts.

59 mics, and is the major catastrophe factor in egg extracts.

60 are defective for DNA replication in Xenopus egg extracts.

61 len- and abasic site-induced ICLs in Xenopus egg extracts.

62 inetochore-microtubule attachment in Xenopus egg extracts.

63 n with PP1 is disrupted in mitotic cells and egg extracts.

64 y in response to oxidative stress in Xenopus egg extracts.

65 interaction zones between asters in Xenopus egg extracts.

66 othesis with model DNA substrates in Xenopus egg extracts.

67 ocalization, and spindle assembly in Xenopus egg extracts.

68 in tissue culture cells but not in X. laevis egg extracts.

69 slation, leads to spindle defects in Xenopus egg extracts.

70 omes with a site-specific barrier in Xenopus egg extracts.

71 during perturbed DNA replication in Xenopus egg extracts.

72 ryonic isoform of linker histone H1 (H1M) in egg extracts.

73 h zero-background interference to be made in egg extracts.

74 o, and loads Cdc45 onto chromatin in Xenopus egg extracts.

75 hase chromosome alignment defects in Xenopus egg extracts.

76 endent but ATM-independent manner in Xenopus egg extracts.

77 In egg extracts, a configuration in which outer kinetochore

78 55 delta (in interphase) from 'cycling' frog egg extracts accelerated their entry into mitosis and ke

79 In egg extracts, activation of Chk1 checkpoint kinase requi

80 Our results show that in Xenopus egg extracts, aggregation of multiple replication forks

81 In egg extracts alanine mutation of the DUE-B C-terminal ph

82 breaks and stalled replication forks in both egg extract and human cells, specifically colocalizing w

83 -3-3zeta dissociation from caspase-2 in both egg extract and human cultured cells.

84 bly of spindle and spindle matrix in Xenopus egg extract and in mammalian cells.

85 Using Xenopus egg extract and in vitro reconstitution systems, here we

86 Through experiments in egg extract and reconstitution with purified proteins, w

87 Additional experiments in Xenopus egg extracts and artificially crowded in vitro solutions

88 Using Xenopus laevis egg extracts and biochemical reconstitution, we found th

89 Using Xenopus egg extracts and biochemical reconstitution, we found th

90 s associate with B-Raf at mitosis in Xenopus egg extracts and contribute to its phosphorylation.

91 for mitotic chromosomal SUMOylation in frog egg extracts and demonstrated that it can mediate effect

92 P2A:B55delta), during mitotic cycles in frog-egg extracts and early embryos.

93 d instead functions to enhance H1 binding in egg extracts and embryos.

94 e significantly reduced PARP1 SUMOylation in egg extracts and enhanced the accumulation of species de

95 e previously studied this process in Xenopus egg extracts and established Greatwall (Gwl) as an impor

96 m-mediated spindle assembly assay in Xenopus egg extracts and extensive mutagenesis studies, we have

97 tudy the metaphase spindle in Xenopus laevis egg extracts and found that microtubules are shortest ne

98 that acts on model CDK substrates in Xenopus egg extracts and has antimitotic activity.

99 lated to S. pombe Cdt2, functions in Xenopus egg extracts and human cells to destroy the replication

100 Using Xenopus egg extracts and human cells, we show that the tumor sup

101 dk for this interaction using both X. laevis egg extracts and human cells.

102 epletion of PP1 impairs NHEJ in both Xenopus egg extracts and human cells.

103 1N is unable to support resection in Xenopus egg extracts and human cells.

104 Here, using Xenopus egg extracts and human somatic cells, we show that actin

105 s DSB-induced ATM activation in both Xenopus egg extracts and human tumor cell lines.

106 adducts were efficiently resected in Xenopus egg extracts and immunodepletion of Xenopus DNA2 also st

107 these oocytes and also causes CSF arrest in egg extracts and in blastomeres of two-cell embryos.

108 Previous studies in Xenopus laevis egg extracts and in highly proliferative cells showed th

109 Using Xenopus egg extracts and in vitro assays, we show that the Xenop

110 rity-independent sliding observed in Xenopus egg extracts and in vitro experiments with purified comp

111 PIASy promotes SUMOylation of PARP1 both in egg extracts and in vitro reconstituted SUMOylation assa

112 rates the mitotic G2/M transition in cycling egg extracts and induces meiotic maturation in G2-arrest

113 ation decreases severing activity in Xenopus egg extracts and is involved in controlling spindle leng

114 Addition of the Mre11 inhibitor mirin to egg extracts and mammalian cells reduces RCC1 associatio

115 We showed in both Xenopus laevis egg extracts and mammalian cells that a conserved cystei

116 duced S-phase checkpoint response in Xenopus egg extracts and mammalian cells.

117 Here, using Xenopus egg extracts and mass spectrometry, we identify SMARCAL1

118 ion from both interphase and mitotic Xenopus egg extracts and mass spectrometry.

119 ules during metaphase in both Xenopus laevis egg extracts and mitotic human cell extracts.

120 By combining studies in Xenopus laevis egg extracts and mouse embryonic fibroblasts (MEFs), we

121 t by dynein and actomyosin forces in Xenopus egg extracts and observed outward co-movement of MTs, en

122 fies chromosomal proteins in mitotic Xenopus egg extracts and plays an essential role in mitotic chro

123 oci or a single chromosomal locus in Xenopus egg extracts and show that a complex library can target

124 f the MAPK cascade during mitosis in Xenopus egg extracts and showed that B-Raf activation is regulat

125 studied assembly of chromatin using Xenopus egg extracts and single DNA molecules held at constant t

126 ited to a DSB-mimicking substrate in Xenopus egg extracts and sites of laser microirradiation in huma

127 ink between DNA replication and N/C ratio in egg extracts and suggest a mechanism that may influence

128 smin binds SENP3 and SENP5 in Xenopus laevis egg extracts and that it is essential for stable accumul

129 reconstruction microscopy (STORM) to Xenopus egg extracts and tissue culture cells, we report various

130 ic chromosome architecture in Xenopus laevis egg extracts and, unlike core histones, exhibits rapid t

131 In Xenopus egg extracts And-1 is loaded on the chromatin after Mcm1

132 laevis and human tissue culture cells, frog egg extracts, and budding yeast.

133 s transiently activated after adding Ca2+ to egg extracts, and inhibitors of calcineurin such as cycl

134 branous spindle matrix isolated from Xenopus egg extracts, and it is required for proper spindle morp

135 RIR of a site-specific ICL lesion in Xenopus egg extracts, and that both its catalytic activity and U

136 BubR1 associates with APC and EB1 in egg extracts, and the complex formation is necessary for

137 reveals that microtubule dynamics in Xenopus egg extracts are unaffected by maskin depletion.

138 tic activation, using cyclin-treated Xenopus egg extracts as a model system, and presented evidence t

139 Using Xenopus egg extracts as a vertebrate model system, we showed pre

140 We have used Xenopus egg extracts as an in vitro system to study the role of

141 In Xenopus egg extract assays, we showed that poly(ADP-ribose) poly

142 In Xenopus egg extracts, ATM associates with TopBP1 and thereupon p

143 t occur at mitotic entry and exit in Xenopus egg extracts back to their origins.

144 ng assay wherein nuclei assembled in Xenopus egg extract become smaller in the presence of cytoplasmi

145 ow that, in both mammalian cells and Xenopus egg extracts, BRCA1/BARD1 is required for mitotic spindl

146 is required for maintenance of CSF arrest in egg extracts, but its function in CSF establishment in o

147 otes is inferred from data in Xenopus laevis egg extracts, but its identity remains elusive.

148 ormation in vivo in activated oocytes and in egg extracts, but not in immature or in vitro matured oo

149 ay is suppressed in the cytoplasm of Xenopus egg extract by phosphatases, but that it becomes activat

150 dk2, and sustained cyclin E-Cdk2 activity in egg extracts causes metaphase arrest in the absence of M

151 Further, in egg extracts, Claspin phosphorylation depends on a thres

152 study, graded concentrations of herring gull egg extracts, collected from five Great Lakes breeding c

153 N-APC interacts with Mad2 in Xenopus egg extracts, colon cancer cells, and in vitro with puri

154 Experiments in frog egg extract confirm the main theoretical predictions.

155 Xenopus egg extracts containing a mutant of TopBP1 that cannot b

156 Furthermore, Xenopus egg extracts containing a version of TopBP1 with an inac

157 ys increased binding to ATR-ATRIP in Xenopus egg extracts containing checkpoint-inducing DNA template

158 Egg extracts containing either a mutant of TopBP1 lackin

159 c RCC1/Ran/RanBP1 complex in M phase Xenopus egg extracts controls both RCC1's enzymatic activity and

160 ahymena group I ribozyme embedded in Xenopus egg extract demonstrate the ability of M2-seq to detect

161 Studies in Xenopus egg extracts demonstrate that Repo-Man interacts with AT

162 analysis using human DR-GFP cells or Xenopus egg extract demonstrated that MCM8 and MCM9 proteins are

163 Polarization microscopy in "cycling" egg extracts demonstrates that de novo centriole formati

164 urther characterization of Pontin in Xenopus egg extracts demonstrates that Pontin interacts with the

165 ear scaling was recapitulated in vitro using egg extracts, demonstrating that titratable cytoplasmic

166 hromosomes accumulate DSBs in Xenopus laevis egg extracts depleted of ATM and ATR.

167 In addition, treatment with egg extract did not enhance expression or stability of J

168 wever, depletion of the protein from cycling egg extracts does not prevent mitotic cell cycle progres

169 During replication in Xenopus egg extracts, DUE-B and Cdc45 bind to chromatin with sim

170 Soluble Xenopus egg extracts efficiently replicate added plasmids using

171 The signal persists in egg extracts even after damaged DNA is removed from the

172 nuclear expansion, in Dppa2-depleted Xenopus egg extracts excess microtubules cause pronuclear assemb

173 ombine our in vitro reconstitution assay and egg extract experiments with computational modeling to s

174 pletion of Greatwall kinase prevents Xenopus egg extracts from entering or maintaining M phase due to

175 We find that egg extracts from H. boettgeri form meiotic spindles sim

176 ton grazer, Daphnia pulicaria, using dormant eggs extracted from sediments in two Minnesota lakes (So

177 In Xenopus laevis egg extracts, GWL-mediated phosphorylation of overexpres

178 h human cells and in vitro data with Xenopus egg extracts have led to the conclusion that the kinase

179 ed illumination microscopy to Xenopus laevis egg extracts, here we reveal that in the absence of micr

180 In Xenopus egg extracts, ICL repair is initiated when two replicati

181 Consistently, in Xenopus egg extracts, Idas-Geminin is less active in licensing i

182 ein interactions in cell-free Xenopus laevis egg extract identified the dimeric histone chaperone fac

183 Immunodepletion of DDK from Xenopus egg extracts impairs chromatin association of Scc2-Scc4,

184 dividing, unperturbed embryos and cell-free egg extract in the absence and presence of DNA damage an

185 s with the Mre11-Rad50-Nbs1 (MRN) complex in egg extracts in a checkpoint-regulated manner.

186 tivity can be induced in Xenopus oocytes and egg extracts in the absence of MAPK or Cdc2 activity.

187 Addition of such an N-APC mutant of egg extracts inactivates the mitotic checkpoint.

188 reconstitution experiments in Xenopus laevis egg extracts indicate that NCOA4 acts as an inhibitor of

189 Overexpression of Pnuts in Xenopus egg extracts inhibited both mitotic and meiotic exit.

190 Depletion of the XCdc7/Drf1 from egg extracts inhibited DNA replication, whereas depletio

191 (Cyclin B1-CDK1) is used to drive interphase egg extracts into a mitotic state, the replicative CMG (

192 nerated by encapsulating cytoplasmic Xenopus egg extracts into cell-sized 'water-in-oil' droplets.

193 onse to stalled replication forks in Xenopus egg extracts involves a complex pathway containing ATM a

194 ase-2, the initiator of apoptosis in Xenopus egg extracts, is associated with an accumulation of LCFA

195 We have observed that egg extracts lacking the Mre11-Rad50-Nbs1 (MRN) complex

196 6P) through the pentose phosphate pathway in egg extracts maintains NADPH levels and calcium/calmodul

197 zation of microtubule flux in Xenopus laevis egg extract meiotic spindles.

198 glass-slide and coverslip in a Xenopus frog egg extract motility assay.

199 Using Xenopus egg extracts, nuclei have been assembled and then induce

200 Binding of Treslin to chromatin in egg extracts occurs independently of TopBP1.

201 fferences between meiotic spindles formed in egg extracts of two frog species.

202 ld generate the shorter spindles observed in egg extracts of X. tropicalis compared to X. laevis.

203 mRNAs copurify with mitotic microtubules in egg extracts of Xenopus laevis.

204 Significantly, removal of Claspin from egg extracts only partially abrogates the activation of

205 Depletion of Bub1 from Xenopus laevis egg extract or from HeLa cells resulted in both destabil

206 Addition of purified Ddk to Xenopus egg extracts or overexpression of Dbf4 in HeLa cells dow

207 ly and organization of microtubule arrays in egg extracts, our studies suggest that Pontin has a mito

208 eflection fluorescent microscopy and Xenopus egg extracts, Petry et al. demonstrate that new microtub

209 ecombinant RSK and endogenous RSK in Xenopus egg extracts phosphorylate all three isoforms of human C

210 critical for H3T3 phosphorylation in Xenopus egg extracts, Plk1 and Aurora B both promote this modifi

211 TM and Aven overexpressed in cycling Xenopus egg extracts prevented mitotic entry and induced phospho

212 Immunodepletion of CENP-C from metaphase egg extract prevents kinetochore formation on sperm chro

213 Imaging of single filaments in Xenopus egg extract provided evidence that disassembly by bursti

214 In Xenopus egg extracts, recombinant L also inhibits mitotic spindl

215 We show that X. tropicalis egg extracts reconstitute the fundamental cell cycle eve

216 After incubation in egg extract, reconstituted CENP-A chromatin specifically

217 Using a Xenopus laevis egg extract replication system, we previously demonstrat

218 timulated to initiate replication in Xenopus egg extracts, replication initiated without any detectab

219 Immunodepletion of Pnuts from egg extracts revealed its essential functions in mitotic

220 ly embryo equivalent (pronuclei incubated in egg extract), S3 neurula cells, A6 kidney cells, and ery

221 he major Th2-inducing component from soluble egg extract (SEA) as the secreted T2 ribonuclease, omega

222 Egg extracts should be prepared in 1 d and can be stored

223 or of mitotic entry, and new work in Xenopus egg extracts shows that Greatwall is required for the po

224 In egg extracts, single DNA molecules assemble into nucleos

225 odel by titrating dynein activity in Xenopus egg extract spindles and quantifying the shape and micro

226 Depletion of Treslin from egg extracts strongly inhibits chromosomal DNA replicati

227 idual activities, and how the Xenopus laevis egg extract system has been utilized as a powerful inter

228 nt degradation both in vivo and in a Xenopus egg extract system in vitro.

229 this study, we took advantage of the Xenopus egg extract system to address these questions.

230 ulators, we developed an assay using Xenopus egg extract that recapitulates the activation of transcr

231 However, Claspin-depleted egg extracts that have been reconstituted with these mut

232 magnetic beads and then incubated in Xenopus egg extracts that provide a source for centromere and ki

233 Using frog egg extracts that recapitulate NHEJ, we show that end pr

234 Using Xenopus egg extracts that recapitulate replication-coupled DPC p

235 del system using ssDNA templates and Xenopus egg extracts that recapitulates eukaryotic G4 replicatio

236 describe a cell-free system based on Xenopus egg extracts that supports ICL repair.

237 monstrate biochemically using Xenopus laevis egg extracts that the Cdk1-counteracting phosphatase PP2

238 In Xenopus egg extracts, the collision of replication forks with in

239 In Xenopus egg extracts, the embryonic linker histone H1M does not

240 Here we demonstrate that in Xenopus laevis egg extracts, the MRN complex is not required for classi

241 pted to reconstitute this process in Xenopus egg extracts, the only eukaryotic in vitro system that r

242 Upon exposure to Xenopus egg extracts, this DNA underwent extensive replication b

243 microfluidics, hydrogels, and Xenopus laevis egg extract to investigate the mechanics of aster moveme

244 Here, we use Xenopus laevis egg extract to investigate the role of the intrinsically

245 In this study we have used Xenopus laevis egg extracts to analyse Uhrf1 function in DNA replicatio

246 ombined microfluidic technology with Xenopus egg extracts to characterize spindle assembly within dis

247 tablished cell-free assays in Xenopus laevis egg extracts to deconstruct the FA pathway in a fully re

248 In this study, we use Xenopus laevis egg extracts to determine the requirements for 9-1-1 loa

249 We have used Xenopus laevis egg extracts to drive an accelerated replication timing

250 We use cell-free Xenopus laevis egg extracts to examine the recruitment of proteins to c

251 In this study, we used Xenopus egg extracts to form spindles in the absence of chromati

252 Here, we have used Xenopus egg extracts to investigate Aur-A's contribution to cell

253 We used Xenopus egg extracts to recapitulate DNA replication invitro.

254 We use Xenopus egg extracts to recapitulate DPC repair in vitro and sho

255 We used Xenopus laevis egg extracts to show that homogenized interphase egg cyt

256 ing assays and functional studies in Xenopus egg extracts to show that TopBP1 makes a direct interact

257 use repair of a site-specific ICL in Xenopus egg extracts to study the mechanism of lesion bypass.

258 We use Xenopus egg extracts to study the nucleation and dynamics of MTs

259 ate-related defense responses, we found that egg extract treatment strongly diminished MYC protein le

260 Fractionation of the egg extract used for nuclear assembly identified a high

261 re of Listeria actin tails in Xenopus laevis egg extracts using cryo-electron tomography.

262 ignal that triggers CMG unloading in Xenopus egg extracts using single-molecule and ensemble approach

263 assembly in tissue culture cells and Xenopus egg extracts using two-photon microscopy with FLIM measu

264 DSB templates that were repaired in Xenopus egg extracts via the canonical, Ku-dependent NHEJ pathwa

265 Here, using Xenopus laevis egg extract we show that MRN-dependent processing of DSB

266 Using Xenopus laevis egg extract we show that Tipin is required for DNA repli

267 Using Xenopus laevis egg extract, we found that increases in cytosolic calciu

268 By using Xenopus laevis egg extract, we found that SUMOylation of DNA topoisomer

269 Using Xenopus laevis egg extract, we have shown that blocking polyubiquitylat

270 Using Xenopus egg extract, we show that direct, cell-cycle-regulated b

271 proteins recruited to DSBs in Xenopus laevis egg extract, we show that DSB-containing DNAs accumulate

272 Using Xenopus egg extracts, we analyzed the functions of FANCM in repl

273 Using Xenopus laevis egg extracts, we demonstrate that Plx1, the Xenopus orth

274 Using Xenopus egg extracts, we describe here a replication-coupled ICL

275 rough biochemical analysis in Xenopus laevis egg extracts, we establish that the MRN (Mre11, Rad50, a

276 Using different assays in Xenopus egg extracts, we found that depleting lamin B caused for

277 MPM-2 epitope kinases in Xenopus oocytes and egg extracts, we have determined that phosphorylation of

278 Using a proteomic screen in Xenopus egg extracts, we identified factors that are enriched on

279 nuclei from transcriptionally silent Xenopus egg extracts, we identified numerous actin regulators, a

280 Using Xenopus egg extracts, we identify an acidic residue in PCNA that

281 Using Xenopus egg extracts, we identify two sequence elements in CRL4(

282 T-Cdc25C phosphorylating activity in Xenopus egg extracts, we previously defined roles of MAPK and Cd

283 Using Xenopus egg extracts, we previously showed that replication fork

284 from and antibody addition to Xenopus laevis egg extracts, we show that BubR1 and its kinase activity

285 Using Xenopus egg extracts, we show that DNA replication at high N/C r

286 Using Xenopus laevis egg extracts, we show that DNA replication continues at

287 nd spindle assembly assays in Xenopus laevis egg extracts, we show that epsin-induced membrane curvat

288 Working in Xenopus egg extracts, we show that Nudel/NudE facilitates the bi

289 Using X. laevis egg extracts, we show that SSX2IP accumulated at spindle

290 Using Xenopus laevis egg extracts, we show that these excess Mcm2-7 complexes

291 Using Xenopus egg extracts, we show that ultraviolet radiation and aph

292 nd destroyed in Fizzy/Cdc20-depleted Xenopus egg extracts when only the N-terminal domain of Fizzy/Cd

293 Here, we show in Xenopus laevis egg extract, where the gradient is best characterized, t

294 Depletion of MTBP from Xenopus egg extracts, which also removes Treslin, abolishes DNA

295 longer interact with TopBP1 in Nbs1-depleted egg extracts, which suggests that the MRN complex helps

296 Using Xenopus egg extracts, which support replication-coupled ICL repa

297 e activation, and supplementation of Xenopus egg extract with glucose-6-phosphate, which promotes cas

298 s in tissue culture cells and Xenopus laevis egg extracts with a mathematical model.

299 By incubating frog egg extracts with supported lipid bilayers containing ph

300 Immunodepletion of xCep57 from egg extracts yields weakened and elongated bipolar spind