ライフサイエンスコーパス: eluent

1 tions (i.e., packing type, ionic strength of eluent).

2 eratures, and low cosolvent fractions in the eluent.

3 n of the metal composing the chromatographic eluent.

4 is more sensitive, faster and consumes less eluent.

5 h-temperature elution with water as the sole eluent.

6 L of 1 mol/L HCl and 5% thiourea was used as eluent.

7 through method with fully aqueous medium as eluent.

8 ough method with fully aqueous medium as the eluent.

9 he crystallization solvent and xylene as the eluent.

10 ivatives were chromatographed using a binary eluent.

11 as the packing material with methanol as the eluent.

12 tial organic content of the second-dimension eluent.

13 nt upon the type and composition of the bulk eluent.

14 ly differed from the composition of the bulk eluent.

15 the variation of the internal energy of the eluent.

16 d to a microelectrode immersed in the column eluent.

17 organic solvent immiscible with the aqueous eluent.

18 to quantitate the metal composition of each eluent.

19 erial as a binding agent, and methanol as an eluent.

20 5mL of 1mol/L HCl was used as eluent.

21 suppressed at 20-30% of acetonitrile in the eluent.

22 gel using hexane-EtOAc solvent system as the eluent.

23 exclusion mode (ICE) using only water as the eluent.

24 atography in a variety of acetonitrile/water eluents.

25 ate, hydroxide and carbonate as suppressible eluents.

26 rent compositions of electrolytes in aqueous eluents.

27 ent in the stationary phase for organic-rich eluents.

28 the subcritical/supercritical CO(2)/alkanol eluents.

29 ar additives in alcohol modifier/CO(2) based eluents.

30 f magnitude over the tested range of aqueous eluents.

31 Buffered eluents (0.1 M ammonium acetate) were necessary to stabi

32 The minor portion of the eluent (10%) was taken for oa-TOFMS for identification.

33 s washing solvent, 150 uL of acetonitrile as eluent, 100 uL of sample at pH 2.5, five draw-eject cycl

34 ator elution, the ammonium acetate generator eluent (15-20 mL) is passed through a tandem IC-H Plus c

35 ease in fatty acids released into the venous eluent (29.4 nmol/ml in transgenic versus 1.35 nmol/ml o

36 The bulk of the eluent (90%) from the UV detector was directed to an ICP

37 methodologies by effective pH control of the eluent (95% phosphate buffer: 5% methanol, v/v) to the n

38 cetate with 0.01% of trifluoroacetic acid as eluent A and acetonitrile as eluent B at a flow rate of

39 pH, contact time, type and concentration of eluent, adsorption capacity, sample volume and interfere

40 at the nature, ionic strength, and pH of the eluent affected protein retention.

41 sample and eluent, volume of the sample and eluent, amount of chelating resin, and interference of i

42 n of an electrolytically generated hydroxide eluent and an electrolytic suppressor, the eluent is pas

43 hat when the percent organic modifier in the eluent and column temperature are adjusted to keep reten

44 tions (type, concentration and volume of the eluent and desorption time), sample volume and interferi

45 ns or ionizable compounds with pure water as eluent and detecting them in a simple fashion has been a

46 lbenzene) column using 1-10% acetonitrile as eluent and follows the reverse order of the polar surfac

47 We varied pH of the eluent and hydrophobicity of ion-pairing modifier to ach

48 l a challenging task since only water as the eluent and no organic modifiers can be used to drive the

49 ive better thermal equilibration between the eluent and the column compared to wider bore columns.

50 he temperature mismatch between the incoming eluent and the column must be minimized (<5 degrees C),

51 of the heat and energy exchanged between the eluent and the external surroundings during each transfo

52 of organic solvent modifier employed in the eluent and the pH of the buffer system.

53 were performed both in common reversed-phase eluents and environmental friendly ethanol-based alterna

54 d to manually prepared carbonate-bicarbonate eluents and with considerable savings in time.

55 extraction buffers, phase-separates from the eluent, and does not reduce NA yield (measured by digita

56 rial that is consumed in these assays is the eluent, and hence, the operation cost is low.

57 required less than 32 mg of sample, 50 mL of eluent, and less than 4 h to complete the measurement of

58 ype, concentration, volume, flow rate of the eluent; and matrix effects on the retention of the metal

59 etention when solvent is added to NaCl-based eluents; and (iii) suppression of much of the column's h

60 A suppressed hydroxide eluent anion chromatograph effluent flows through the ou

61 is of competitive Langmuir isotherms, if the eluent anion is more strongly retained than the analyte

62 If the charge of the analyte and eluent anions are different (e.g., Br(-) vs CO3(2-)), th

63 The eluents are detected using a unique spectrometer equippe

64 ained by the use of external gradient of the eluent, are brought about by the formation of an interna

65 ated the length of tubing needed to heat the eluent as a function of the column linear velocity for b

66 determined by IAM-HPLC in any 10 mM buffered eluent at pH 5.

67 actions when CH3CN is used with NaClO4-based eluents at a neutral pH (i.e., this eluent system separa

68 11min with UV and MS compatible, buffer-free eluents at moderate temperature.

69 oacetic acid as eluent A and acetonitrile as eluent B at a flow rate of 0.2 mL min(-1).

70 e of both phosphate and fluoride ions in the eluent, band broadening caused by Lewis acid/base intera

71 lic-phase monolithic columns, with capillary eluent being deposited on a standard MALDI plate along w

72 rates, type, concentration and volume of the eluent, breakthrough volume, and effect of other ions we

73 ate or other strong, hard Lewis bases to the eluent brings about elution, but the resulting peak is o

74 uenced by the electrolyte composition of the eluent but with a consistent trend for a diverse set of

75 y was used to investigate the uptake of RPLC eluents by a C 18-bonded packing.

76 approach also opens the possibility that an eluent can be individually tailored to meet the specific

77 eric factors while elution was influenced by eluent cation complexation.

78 s and 4 microL/min water flowing through the eluent channel, with a reverse bias of -12 V, the leakag

79 sign these devices do not produce gas in the eluent channel; hence, it is not necessary to remove gas

80 In hydroxide eluent chromatography, the device largely removes the re

81 the number of fractions of the first column eluent collected, and the analysis time of the first dim

82 and support material, interfering ions, best eluent, column reusability were studied.

83 e unretained dead time marker for water-rich eluents combined with the regression results from excess

84 an ammonium acetate buffered methanol-water eluent, compatible with mass spectrometry (MS).

85 pH 7 from sample volume up to 400mL and then eluent completely with 2mL of 0.5molL(-1)HNO3.

86 as used to measure the excess volume of each eluent component for binary and ternary mixtures of wate

87 sed to determine the absolute volume of each eluent component in the stationary phase as a function o

88 direct measurement of excess volumes of each eluent component without numerical integration, assumed

89 used to simultaneously measure the uptake of eluent components by a C(18)-bonded reversed-phase liqui

90 to detecting capillary electrophoresis (CE) eluent components by interfacing CE with a surface-enhan

91 as to determine whether or not the uptake of eluent components immobilized as part of the stationary

92 The results indicate that all three eluent components interacted with the alkane bonded phas

93 The absolute sorption isotherms of the eluent components were indirectly estimated by a combina

94 ined were excess volumes of sorption for the eluent components.

95 re performed in a quartz sand column with an eluent composed of 10(-2) M NaCl at a pH of 7.5.

96 ects of key experimental parameters, such as eluent composition and elution pressure, on separation e

97 zed conditions of aqueous acetonitrile (ACN) eluent composition and temperature are established for t

98 , and tetrahydrofuran over the full range of eluent composition at 25 degrees C.

99 dence of perfluoromethylene selectivity upon eluent composition explains the typical reversed-phase b

100 ries of test analytes over the full range of eluent composition for methanol and acetonitrile with wa

101 the stationary phase but only over a limited eluent composition range.

102 g a series of strategies specific to limited eluent composition range.

103 rease, which necessitates careful control of eluent composition to achieve separation of all the taxa

104 The shift of the critical eluent composition with the monomer composition of the p

105 ample loading flow rate, rinsing volume, and eluent composition), we found that A(20)R(20)-AuNPs (R(2

106 se could be estimated over the full range of eluent composition.

107 hase volume but only over a limited range of eluent composition.

108 tor calibration, or off-line analysis of the eluent composition.

109 variation of the particle properties and the eluent composition.

110 For the C8 phase with eluent compositions in the 40%-60% ACN range, the k' val

111 Interestingly, most of the supposedly mild eluent compositions induced nonideal SEC behavior and/or

112 The higher retention on the latter requires eluent compositions near 50% ACN, where careful temperat

113 s were used and evaluated for the water-rich eluent compositions.

114 ly 350 S.cm2/equiv) of H+ and relatively low eluent concentration allows sensitive conductometric det

115 (2-)), the analyte peak shapes depend on the eluent concentration in a more complex pattern.

116 The permeation flux and thus the eluent concentration is controlled by varying the applie

117 ), the availability of effective methods for eluent concentration is important.

118 ection volume, carrier stream flow rate, and eluent concentration on system response.

119 s of altering and extending the initial NaOH eluent concentration on the retention of 42 different ca

120 rge on the ion and (b) at the same hydroxide eluent concentration, retention is greatly dependent on

121 pH, amount of tea waste, extraction time and eluent concentration.

122 and can easily generate significantly higher eluent concentrations (at least to 40 mM carbonate), pav

123 ng to be used over long periods at practical eluent concentrations, paving the way for suppressed hyd

124 For practical eluent concentrations, suppressor active lengths less th

125 However, whether the applied eluent conditions actually prevent protein-stationary ph

126 The experiments were carried out with eluents consisting of binary aqueous mixtures with aceto

127 sed on maintaining the ionic strength of the eluents constant.

128 hanced in terms of reduced analysis time and eluent consumption with respect of classical HPLC method

129 med by HPLC on 5-mum Zorbax SB-CN column and eluent containing 40% acetonitrile (v/v), 20 mM phosphat

130 old PFP, acetonitrile/methanol based aqueous eluents containing either phosphoric or perchloric acid

131 old PFP, acetonitrile/methanol based aqueous eluents containing phosphoric acid) as preferred methods

132 The resulting eluent contains NAs and carryover of extraction buffers.

133 h the external wall of the column during the eluent decompression was estimated by measuring the surf

134 The results showed that, during the eluent decompression, the heat released by the friction

135 At low eluent dilution (2-2.5x) we observed significant reactio

136 At certain experimental conditions, the eluent divided into two phases, both of which moved thro

137 ion mass spectrometry (LC-ESI-MS), where the eluent does not contain any ion-pairing reagent (IPR).

138 y used to separate basic compounds in acidic eluents due to their high efficiency, good mechanical st

139 H (1.5-3), ultralow-pH (0), and high-pH (12) eluents effect the retention properties of these mixed-m

140 Ultralow-pH eluents effectively separate small peptides on both phas

141 our different purposes: (1) it increases the eluent entropy at constant temperature (for approximatel

142 le 2D peak capacity is maximum for a certain eluent flow rate and column length of the second-dimensi

143 The effects of the eluent flow rate and composition as well as the nebulize

144 The effects of the eluent flow rate and composition as well as the nebulize

145 ptic digest of bovine serum albumin using an eluent flow rate of 55 muL min(-1).

146 ionic strength, elution solution, sample and eluent flow rates, and sample volume were determined.

147 e sample solution, type and concentration of eluent, flow rates of the sample and eluent, volume of t

148 (anionic and nonionic surfactants) micellar eluent for determination of the total PET radioligand co

149 flow rates, type and the smallest amount of eluent for elution of cadmium ions, break through volume

150 pendant droplet evaporation for exchange of eluents for (1)H nuclear magnetic resonance ((1)H NMR) p

151 5%); (2) it increases the temperature of the eluent (for approximately 5%); (3) it provides heat to t

152 -performance liquid chromatography (RP-HPLC) eluent fractions, either before or after lyophilization.

153 at the end of the (1)D column to monitor the eluent from (1)D and assist in reconstructing (1)D eluti

154 The eluent from the LC column is mixed online with a continu

155 In addition, the liquid eluent from the separation was directed on-line into an

156 nalyte was eluted in back-flush mode and the eluent from the SPE column was diluted through a mixing

157 In this study, the polymer-containing eluent from the ThFFF system was mixed on-line with MALD

158 loys a pulsed electric field to transfer the eluents from multiple parallel columns directly onto MAL

159 y explored in ion chromatography with online eluent generation and suppressed conductivity detection.

160 We introduce a novel carbonate-bicarbonate eluent generation system in which CO2 is introduced usin

161 introduction is possible prior to hydroxide eluent generation, this configuration causes complicatio

162 ped with isocratic pumps and an electrolytic eluent generator (EG) is introduced, replacing external

163 atile than current electrodialytic carbonate eluent generators and can easily generate significantly

164 ated region during their passage; the chosen eluent gradient elutes the analytes of interest into the

165 In addition, eluent gradients were created by simultaneously using tw

166 croscopic levels of the acetate anion of the eluent have been effectively removed.

167 showing the general utility of the union for eluent identification and low-level detection.

168 s in 5' or 3' terminal bases with NaCl-based eluents); (ii) reduced retention when solvent is added t

169 the reaction mix (e.g., 10x dilution is 1 uL eluent in 9 uL reaction mix), but in applications requir

170 e selectively excluded so the composition of eluent in or on the stationary phase often significantly

171 calculate the volume and composition of the eluent in the stationary phase for organic-rich eluents.

172 nmol/ml in transgenic versus 1.35 nmol/ml of eluent in wild-type mice), a 4-fold increase in lysophos

173 the use of 0.1% phosphoric acid/acetonitrile eluents in both dimensions.

174 pillary-scale electrodialytic generators for eluents in ion chromatography are described.

175 to increase the elution strength of aqueous eluents in reversed phase LC is the application of high

176 identical LC settings with formic-acid-based eluents in the last dimension.

177 roach will be of value for the generation of eluents in the separation of proteins and other biomolec

178 mination, such as pH of the sample solution, eluent including type, concentration and volume, adsorpt

179 lysis was accomplished by introducing the GC eluent into a pulsed glow discharge operating at a rate

180 ons, paving the way for suppressed hydroxide eluent ion chromatography (IC), which is discussed in a

181 nsP5, InsP6) was carried out using hydroxide eluent ion chromatography.

182 The eluent is a dilute solution of a neutral salt, sometimes

183 The eluent is initially under atmospheric pressure ( P (0))

184 vity, and the water from the first-dimension eluent is largely eliminated to allow interaction-free S

185 Axial mixing of the sample with a gaseous eluent is minimal, and this eliminates the tailing in pe

186 The eluent is monitored fluorescently at Ex553 nm/Em570 nm b

187 If the eluent is more weakly retained on the stationary phase,

188 e eluent and an electrolytic suppressor, the eluent is passed into a membrane device where KOH is pas

189 The H(2)CO(3)* eluent is prepared in situ by high pressure permeative i

190 irectly determined and MeHg(I) in the second eluent is quantified using FI-CVG-AAS after its oxidativ

191 With this method, the final eluent is suitable for structure-function relationship s

192 The spin column eluent is then analyzed under denaturing conditions by e

193 In a second step, the pressurized eluent is transferred to the inlet of the chromatographi

194 CEC eluent is transported to the matrix reservoir via a capi

195 its very high hydration energy and with most eluents its capacity factor approaches zero.

196 The FAVE provides isolation from the eluent matrix and can be used for other detectors where

197 nd can be used for other detectors where the eluent matrix is incompatible with the detector.

198 pH, adsorbent amount, contact time, type of eluent, matrix and reusability) affecting the extraction

199 orisidine upon preparative TLC purification (eluent: MeOH/CHCl(3) saturated with NH(3)) and equilibri

200 ation, retention is greatly dependent on the eluent metal cation.

201 or column (PFC kit) was installed in between eluent mixer and injector to reduce contamination.

202 rates would be collected with the NPs in the eluent of F4.

203 Each peak was collected as the eluent of the HPLC separation in the liquid phase.

204 n the nanotubes were sequentially exposed to eluents of decreasing electrolyte concentrations, possib

205 aCl2 concentrations before being rinsed with eluents of different solution chemistries to induce thei

206 oving the ion-pair reagent, the detection of eluents of monoamine neurotransmitters by an ion trap MS

207 nd mobile phases in dynamic equilibrium with eluents of varying composition.

208 obin was used as the main model protein, and eluents of varying ionic strength and pH were applied.

209 The effect of this uptake of eluent on the retention of the test solutes appeared to

210 c sampling (injection) of the primary column eluent onto the secondary column.

211 tor of 2-3 of best case suppressed hydroxide eluent operation.

212 For late-eluting AAs, higher eluent organic content and fraction collected volumes co

213 splay of stationary phase retention (Sf) and eluent partition coefficients (K), which represent criti

214 e adjustment of oligonucleotide retention by eluent pH and composition.

215 use of organic modifiers, and variations of eluent pH can be used to tailor a given separation.

216 For a weak base that was tested in eluent pH either above or below its pK(a), we demonstrat

217 Sample/eluent pH matching is advantageous to prevent thioarseni

218 (ICP-MS) are presented that allow for sample/eluent pH matching.

219 ion of the AuNPs was examined by raising the eluent pH to 10.

220 ic carbon (PGC) columns and phosphate buffer eluents (pH 6.7 and pH 9.1, respectively) were developed

221 ules between the ion exchanger phase and the eluent phase and is applied for conductivity suppression

222 carboxylic acids or ammonium hydroxide to LC eluents postcolumn can improve MS sensitivity.

223 oach offered enhanced throughput in terms of eluent preparation time and labor, and with a more repro

224 ovided an estimate of the absolute volume of eluent present in the stationary phase.

225 les provides the necessary functionality for eluent propulsion and sample valving.

226 revent any leached extractant or oxalic acid eluent reagents from interfering with subsequent separat

227 ear velocity in the heater tubing and longer eluent residence times in the heater.

228 tantly, the use of pH 10 carbonate buffer as eluent resulted in facile release of bound biotin from t

229 pulsion/attraction is strongly influenced by eluent salinity: k(IAM) values for cations differ by mor

230 usion ESI-MS showed that the tested volatile eluent salts seem to follow the Hofmeister series: no de

231 , stirring time, concentration and volume of eluent, sample flow rate and sample volume was examined

232 ed microcolumn and As(III) was determined in eluent solution by electrothermal atomic absorption spec

233 s such as pH, adsorbent amount, mixing time, eluent solution, sample volume, etc.

234 and desorption times, type and volume of the eluent solvent and interfering ions of the sample were i

235 = 2; amount of damped MHAMS-MIONPs = 90 mg; eluent solvent volume = 2.6 mL of 3% acetic acid in acet

236 of tea waste, desorbed with nitric acid as a eluent solvent, and determined by flame atomic absorptio

237 therms provided an estimate of the volume of eluent sorbed by the stationary phase but only over a li

238 excess isotherm data, the absolute volume of eluent sorbed by the stationary phase could be estimated

239 en the HPLC and ICPMS, which consisted of an eluent splitter, a desolvation unit, and the ICPMS built

240 (1) Residual organic carbon in the eluent stream of the interface was determined to calcula

241 e where KOH is passively introduced into the eluent stream using Donnan forbidden leakage.

242 mixing of the penetrated hydroxide with the eluent stream, resulting in a noise level of < or = 7 nS

243 ), flow rate (F), temperature (T), and final eluent strength (phi(final)) on the peak capacity of sep

244 Last, the final eluent strength should be adjusted so that the last solu

245 sion is kept in a no-elution state using low eluent strength.

246 ce (PAID) is placed after a conventional KOH eluent-suppressed conductometric anion chromatography (S

247 The eluent system allows particular latitude in controlling

248 O4-based eluents at a neutral pH (i.e., this eluent system separates oligonucleotides primarily in or

249 hate was used as the ion-pair reagent in the eluent system.

250 Demonstrably purer carbonate-bicarbonate eluent systems are possible compared to manually prepare

251 derivatization or the use of highly alkaline eluent systems.

252 Thermocouples were used to measure the eluent temperature before and after its passage through

253 The aqueous/organic eluents that are used with this stationary phase are ide

254 A variety of eluents, the use of organic modifiers, and variations of

255 For carbonate-based eluents, the use of such devices greatly reduces or elim

256 o acts as a mixing chamber, allowing the CEC eluent to be mixed with matrix prior to deposition.

257 condary dimension enables the primary column eluent to be sampled with fidelity onto the secondary co

258 reheater tubing to thermally equilibrate the eluent to the column temperature.

259 electrophoretic separation channel to direct eluent to the integrated electrospray emitter.

260 d desorption time, maximum capacity and also eluent type and concentration was investigated in this s

261 Analytical parameters such as pH, eluent type and its volume, flow rates of sample solutio

262 are many more choices of column type than of eluent type for method development in reversed-phase liq

263 Effects of various parameters such as pH, eluent type, vortex time, amount of magnetic adsorbent w

264 ple volume, flow rate, adsorbent dosage, and eluent type.

265 ty of gamma-Fe(2)O(3) magnetic nanoparticle, eluent (type, concentration and volume), sample volume,

266 ontaining 1.0 mM sodium tetraborate solution eluent (typically 200 psi) as the pump, and performing o

267 nventional length columns (e.g., 5-15 cm) at eluent velocities corresponding to the minimum plate hei

268 d when particle size is optimized along with eluent velocity and column length.

269 available particle size and then optimizing eluent velocity and column length.

270 lumn temperature improves efficiency at high eluent velocity conditions compared to the efficiency at

271 w rapid calculation of the column length and eluent velocity that will give either the maximum plate

272 by simultaneously optimizing column length, eluent velocity, and particle size.

273 are developed for the optimum column length, eluent velocity, and thus plate count for both the cases

274 We have shown that the 5-10-fold decrease in eluent viscosity that comes from a temperature increase

275 anic modifier to achieve the lowest possible eluent viscosity.

276 ntal analysis experiments, the working time, eluent volume consumed, and mass of compound used were r

277 eters such as sample-to-dispersant ratio and eluents volume on extraction recovery was investigated a

278 dified silica gel, concentration and type of eluent, volume of sample, etc.) on the recoveries of sel

279 tion of eluent, flow rates of the sample and eluent, volume of the sample and eluent, amount of chela

280 The eluent was characterized using mass spectrometry.

281 The eluent was composed of mixtures of methanol and carbon d

282 When the CaCl2 concentration in the eluent was decreased, a larger fraction of deposited MWN

283 Hg(II) in the eluent was directly determined and MeHg(I) in the second

284 The column eluent was dried and derivatized for GC/MS analysis.

285 The ionic strength of the eluent was kept constant at 20 mM.

286 The LC eluent was mixed with alpha-cyano-4-hydroxycinnamic acid

287 (90:10, v/v) at flow rate 1.0mL/min and the eluent was monitored at 219nm.

288 The target peak eluent was subjected to characterisation by tandem mass

289 tionary phase (bonded phase plus immobilized eluent) was dependent upon the type and composition of t

290 an alternative to adding Lewis bases to the eluent, we studied the effect of permanently modifying P

291 ressibility and the thermal expansion of the eluent were taken into account.

292 HILIC and RPLC eluents were combined post column followed by ESI-MS/MS

293 rvasive carryover of inhibitory buffers into eluent when several commercial sample-preparation kits a

294 or in function of acetonitrile amount in the eluent, whereas retention of neutral molecules decreased

295 an achiral stationary phase using an achiral eluent, which leads to the substantial enantiomeric enri

296 ary phase using a carbonic acid (H(2)CO(3)*) eluent with and without a mineral acid.

297 or bilinear pH-gradient data set obtained in eluents with different but constant organic modifier con

298 xchange using carbonate-bicarbonate and NaOH eluents with step-gradient elution.

299 ata, particularly comparisons with an HNO(3) eluent, with or without H(2)CO(3)*, indicate that proton

300 and up to 100% organic components in the LC eluent without organic loading in the plasma.