ライフサイエンスコーパス: endolysin

1 ells by the enzymatic action of the released endolysin.

2 ly becomes permeabilized to the fully folded endolysin.

3 skin by S. aureus is profoundly inhibited by endolysin.

4 Lysogens of Ef11 are resistant to the Ef11 endolysin.

5 ng fusion to murein degradation by the phage endolysin.

6 es specificity of the catalytic domain of an endolysin.

7 lysin of Erwinia phage ERA103, is also a SAR endolysin.

8 lysis by bacteriophage encoding a secretory endolysin.

9 letely incompetent for lysis with the hybrid endolysin.

10 al architecture of therapeutic antibacterial endolysins.

11 h engineered bacteriophage S aureus-specific endolysins.

12 ntly of, but in conjunction with, holins and endolysins.

13 determines the recognition by bacteriophage endolysins.

14 d subsequent staphylolytic activity for some endolysins.

15 e two proteins for efficient host lysis: the endolysin, a muralytic enzyme, and the holin, a small me

16 igomers form membrane lesions, through which endolysin, a muralytic enzyme, escapes the cytoplasm to

17 Pinholins require secreted SAR endolysins, a pairing that may represent an intermediate

18 cheduled time after infection and allows the endolysin access to its substrate, resulting in host cel

19 uralytic enzymes that degrade the cell wall; endolysins accumulate in the cytosol fully folded during

20 ction cycle in which intracellular phage and endolysin accumulation can continue for hours.

21 Since endolysin activity is dependent upon binding to cell sur

22 tional study in pigs to assess the impact of endolysin administration on the microbiome.

23 lead variant possessed an N-terminal modular endolysin and a C-terminal lipase.

24 Two proteins, an endolysin and a holin, are essential for host lysis by b

25 a lytic process requiring, at a minimum, an endolysin and a holin.

26 e that encodes an outer membrane lipoprotein endolysin and also spanin gene families that provide ins

27 tion site change the oligomeric state of the endolysin and reduce lytic activity.

28 an transglycosylase related to bacteriophage endolysins and acts as an autolysin in the stationary ph

29 rnative mode of treatment both bacteriophage endolysins and bacteriocins have been shown to possess a

30 tail the structural diversification of phage endolysins and confirm their potential in eliminating gu

31 membrane, leading to activation of secreted endolysins and murein degradation.

32 lish host lysis using a muralytic enzyme, or endolysin, and a holin, which permeabilizes the membrane

33 pinholin, a signal anchor and release (SAR) endolysin, and Rz and Rz1 equivalents.

34 Endolysins are peptidoglycan hydrolases produced at the

35 k the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where th

36 PGHs of bacteriophage origin, called endolysins, are involved in bacterial lysis at the end o

37 ll aid future work aiming to engineer better endolysins as safe food antimicrobials.

38 ay have broad implications for the design of endolysins as therapeutic agents.

39 SAR endolysins become activated by various mechanisms upon r

40 Toyofuku et al. show that a prophage-encoded endolysin can generate holes in the cell wall through wh

41 This is the first report that a native endolysin can traverse epithelial membranes, thus substa

42 dulated by reconstitution of the full-length endolysin-CBD complex with free CBD.

43 Endolysin CD27L causes cell lysis of the pathogen Clostr

44 uring infection, the truncated bacteriophage endolysin CHAPK and the staphylococcal bacteriocin lysos

45 present a crystal structure of the activated endolysin CTP1L that targets Clostridium tyrobutyricum,

46 bited a holin-like function by promoting the endolysin-dependent lysis of an induced lambda lysogen t

47 In this study, an endolysin derived from vB_AbaM_PhT2 (vPhT2), was identif

48 as not due to the proteolytic release of the endolysin domain of the R-beta-Gal fusion protein.

49 hage T4 effects lysis by its holin T and its endolysin E.

50 4 effects host lysis with a holin, T, and an endolysin, E.

51 ort further development of S aureus-targeted endolysins for future clinical management of skin and wo

52 herefore, it is proposed that specificity of endolysins for specific bacilli is achieved by selective

53 has implications for the design of effective endolysins for the treatment of bacterial infections.

54 Leu 98 to a Trp residue which is found in an endolysin from a bacteriophage of Listeria monocytogenes

55 unprecedented size (>300 nm), releasing the endolysin from the cytoplasm, resulting in lysis within

56 enough to allow release of prefolded active endolysin from the cytoplasm, which results in destructi

57 The other, AtlA, is similar to endolysins from bacteriophages and is not similar to any

58 Because endolysin function requires the formation of mum-scale h

59 ration of the endolysin receptor, preventing endolysin function.

60 coexpressed with Rz1, despite the absence of endolysin function.

61 When the CTP1L endolysin gene is introduced into the commensal bacteriu

62 n internal translation start site within the endolysin gene.

63 , three new putative intron-containing phage endolysin genes were identified in public data sets for

64 sis cassette, (ii) constitutively expressing endolysin genes while restricting holin genes, and (iii)

65 eriophage lambda requires only the holin and endolysin genes, but not the Rz and Rz1 genes, of the ly

66 tein (gp17), the phage integrase (gp29), the endolysin (gp31), the phage repressor (gp47), and six pr

67 R, the endolysin, has muralytic activity.

68 Endolysins have acquired stringent substrate specificiti

69 the holin and antiholin, and R, encoding the endolysin, have been intensively studied, the products o

70 ow that the expression of a prophage-encoded endolysin in a sub-population of cells generates holes i

71 havior, causing bacterial lysis by releasing endolysin in an oligomerization-dependent manner.

72 Endolysins in Gram-positive phages come in a variety of

73 e membrane were nonpermissive for the hybrid endolysin, indicating that these premature lesions const

74 Moreover, endolysin inhibits the patient-derived S. aureus inducti

75 After peptidoglycan digestion with phage endolysin, InlA-MH(6)-Cws was purified by affinity chrom

76 uncated T holin functional in lysis with the endolysin is completely incompetent for lysis with the h

77 Thus, the exported P1 endolysin is kept inactive by three levels of control-to

78 Endolysin is the term for muralytic enzymes that degrade

79 We have characterized two endolysins, Lc-Lys and Lc-Lys-2, identified in prophages

80 These chimeras consist of endolysins linked by polypeptides to diverse phage prote

81 The endolysin-lipase chimera demonstrated specificity toward

82 Putative lysis genes encoding endolysin (Lys) and holin proteins were identified from

83 Recombinant endolysin (lysAB- vT2) and hydrophobic fusion endolysin

84 ndolysin (lysAB- vT2) and hydrophobic fusion endolysin (lysAB-vT2-fusion) were expressed and purified

85 PlyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact

86 doid phage 21 has the prototype pinholin-SAR endolysin lysis system, which is widely distributed amon

87 In contrast, in pinholin-SAR endolysin lysis, the cell began to shorten and thicken u

88 In its inactive form, the prototype SAR endolysin, Lyz(P1), of coliphage P1, has an active-site

89 Our results support the idea that endolysins may be important for MV formation in bacteria

90 ults are discussed in terms of a model where endolysin-mediated degradation of the cell wall is a pre

91 The Lyz endolysin of bacteriophage P1 was found to cause lysis o

92 Here, we report that Lyz(103), the endolysin of Erwinia phage ERA103, is also a SAR endolys

93 ns, and experimental data using the purified endolysin of phage c2, we propose that Audmula modifies

94 e peptidoglycan degrading transglycosylases (endolysins) of bacteriophages lambda and P2, suggesting

95 We further show that a related endolysin (Ply21) from the B. cereus phage, TP21, shows

96 incorporation of the listeria bacteriophage endolysin Ply500: covalent attachment onto FDA approved

97 g Streptococcus pneumoniae LytA and Listeria endolysin Ply511, to understand its specific enzymatic m

98 binding the cell wall binding domains of the endolysins PlyL and PlyG were determined by surface plas

99 ctional analysis of the lambda prophage Ba02 endolysin (PlyL) encoded by the Bacillus anthracis genom

100 we used the Streptococcus dysgalactiae phage endolysin PlySK1249 as a model to investigate the role o

101 Endolysins possess the ability to cleave peptidoglycan a

102 aused by these pores activates the muralytic endolysin, R(21), leading immediately to peptidoglycan d

103 ve SRP function results in alteration of the endolysin receptor, preventing endolysin function.

104 This endolysin represented the conserved lysozyme domain.

105 her lysostaphin (bacteriocin) or LysK (phage endolysin) resulted in a approximately 5x increase in st

106 Both endolysins showed lytic activity against bacterial crude

107 age 21 uses a pinholin-signal anchor release endolysin strategy to effect temporally regulated host l

108 ct mechanism involving nonmalignant T cells, endolysin strongly inhibits the effects of S. aureus on

109 We show that endolysin strongly inhibits the proliferation of S. aure

110 e protein sequences of related bacteriophage endolysins suggests that the presence of an N-terminal s

111 ea and appears to be distinct from the holin/endolysin system described for DNA bacteriophages.

112 Bacteriophage lambda uses a holin-endolysin system for host cell lysis.

113 hages with either the lambda canonical holin-endolysin system or the phage 21 pinholin-signal anchor

114 hage 21 pinholin-signal anchor release (SAR) endolysin system.

115 rmediate in the evolution of canonical holin-endolysin systems.

116 om the GRAS Listeria phage P100, is one such endolysin that can prevent the growth of L. monocytogene

117 igomerization mechanism applies to the CD27L endolysin that targets Clostridium difficile and the CS7

118 targets Clostridium difficile and the CS74L endolysin that targets Clostridium sporogenes.

119 Listeria-specific bacteriophages encode endolysins that can cleave the peptidoglycan layer of L.

120 yz(P1) and Lyz(103) define subclasses of SAR endolysins that differ in the nature of their inhibitory

121 acteria for the primary purpose of releasing endolysins that hydrolyze the cell wall and induce cell

122 Moreover, on coexpression of holin and endolysin, the degradation of the cell wall leads to sph

123 a holin that mediates the movement of the T4 endolysin though the inner cell membrane to its target,

124 This permeabilization enables the R endolysin to attack the cell wall, after which cell lysi

125 ethal membrane lesion which allows the phage endolysin to attack the peptidoglycan.

126 hat AtlA can functionally replace the lambda endolysin to lyse Escherichia coli.

127 y and sufficient not only for export of this endolysin to the membrane but also for its release into

128 We show that truncation of the endolysin to the N-terminal domain, CD27L1-179, gave an

129 a normal signal-arrest domain to direct the endolysin to the periplasm in membrane-tethered form and

130 Delivery of endolysins to the peptidoglycan of gram-negatives is imp

131 specific release of pre-folded, fully active endolysins to the periplasm.

132 ctive cycle, allow bacteriolytic enzymes, or endolysins, to escape to the periplasm and to attack the

133 ccess of phage-encoded muralytic enzymes, or endolysins, to the cell wall by the sudden formation of

134 nding domains of the streptococcal LambdaSa2 endolysin were replaced by staphylococcal SH3b domains f

135 Bacteriophages express endolysins which are the enzymes that hydrolyze peptidog

136 obial ability of engineered lysAB-vT2-fusion endolysin, which can be applied for the control of A. ba

137 e hosts has been shown to require holins and endolysins, which attack the cytoplasmic membrane and pe

138 Bacteriophages produce endolysins, which lyse the bacterial host cell to releas

139 In contrast to canonical phage endolysins, which require holin-mediated disruption of t

140 Overall, we generated a chimeric endolysin with exogenous and specific activity toward Ps

141 A C-terminal fusion of the R endolysin with full-length beta-galactosidase (beta-Gal)

142 However, the reason why phages adopt endolysins with such complex multidomain architecture is

143 Taken together, we provide evidence that endolysin XZ.700 inhibits skin colonization, chemokine e

144 ed, bacteriophage-derived, S aureus-specific endolysin (XZ.700) abrogates the effect of S aureus supe

145 ctivity and efficacy of an S aureus-targeted endolysin (XZ.700) within the complex living skin/wound

146 ect of a recombinant, antibacterial protein, endolysin (XZ.700), on S. aureus skin colonization and m