ライフサイエンスコーパス: engineer

1 de deep tier, high-impact, complex ecosystem engineering.

2 search for ultrastrong metals via materials engineering.

3 rom microarrays and smart surfaces to tissue engineering.

4 rdering has direct applications in metabolic engineering.

5 ve layer with comprehensive bandgap and film engineering.

6 t methods and their applications for peptide engineering.

7 de from standard output with no need for new engineering.

8 emerging as a powerful mode of CRISPR-based engineering.

9 D is a promising material for cardiac tissue engineering.

10 combination of ligand mutagenesis and ligand engineering.

11 is for outlier detection in chemometrics and engineering.

12 tific research, practical manufacturing, and engineering.

13 s well as industrial applications of protein engineering.

14 yanines (PC) that was cationized by chemical engineering.

15 of central importance in quantum science and engineering(1).

16 Rapid advances in cellular engineering(1,2) have positioned synthetic biology to ad

17 entify the barriers that must be overcome to engineer a bacterium to deliver a high nitrogen flux to

18 Here we apply rational design to engineer a complete set of monomeric NIR FPs, which are

19 n of the murine STING CDN binding domain, we engineer a Forster resonance energy transfer (FRET) base

20 Using directed evolution, we engineered a 'decoy-resistant' IL-18 (DR-18) that mainta

21 For pharmacological blockade, we engineered a bispecific receptor decoy by attaching the

22 in skeletal muscle cells to pathogenesis, we engineered a CHIKV strain exhibiting restricted replicat

23 In this study, we engineered a class of type III hammerhead ribozymes to d

24 We engineered a DUB mutation (Asp1772 to Ala) into a murine

25 idence for the presence of these uracils, we engineered a protein that covalently links to DNA at ura

26 We designed embryo traps and engineered a protocol allowing for efficient chemical ex

27 emotherapy and photodynamic therapy, we have engineered a robust and smart "all-in-one" nanoparticle-

28 We have previously engineered a synthetic DNA vaccine targeting the MERS co

29 We therefore engineered a yeast strain expressing a new type of Split

30 ved quantum yields (QY) could be achieved by engineering a protein corona structure consisting of a r

31 as9-mediated genome editing in S. rosetta by engineering a selectable marker to enrich for edited cel

32 interpret the observations made for further engineering alloys with two-phase microstructures.

33 anded microstructures commonly occur in many engineering alloys, the analysis of stress and strain pa

34 To tackle this problem, we engineered an HA tag onto Cx26 or Cx30 subunits and imag

35 y density functional theory calculations for engineering analogs of this class of fluorophores are of

36 fore used computational screening to reverse-engineer and identify these forces.

37 of specificity and cleavage outcomes across engineered and natural nucleases.

38 In this study, we engineered and selected mutant forms of uPlm that are bo

39 To establish sc-FLCCS, we first engineered and tested multiple green fluorescent protein

40 ular construct based on a combinatorial nano-engineering and biomaterial encapsulation approach, coul

41 dy scaffolds, trastuzumab, used for antibody engineering and drug conjugation.

42 r suitability for applications in biomedical engineering and environmental remediation.

43 Coupled with feature engineering and immunological context, researchers can i

44 of engineered macrophages, including genetic engineering and integration with biomaterials or drug de

45 to gender imbalances in science, technology, engineering and mathematics (STEM) fields, among other u

46 ic areas throughout the Science, Technology, Engineering and Mathematics (STEM) pipeline that perpetu

47 hich is central to a variety of domains from engineering and medicine to economics and social plannin

48 driven by technological advances in genetic engineering and metabolism as well as by the realization

49 iomedical applications, especially in tissue engineering and regenerative medicine.

50 iven numerous pivotal advancements in tissue engineering and regenerative medicine.

51 Classical T4 phage engineering and several newly proposed methods are often

52 Here, the holistic engineering and systematic characterization of the impac

53 neration of mutant DNA sequences for protein engineering and the functional analysis of genetic varia

54 Together with breakthroughs in genome engineering and the various omics, organoid technology i

55 gene cassette), vector tropism (using capsid engineering) and the ability of the capsid and transgene

56 can be expensive to produce, challenging to engineer, and are not necessarily stable in the context

57 etric-structure design, electronic-structure engineering, and applications in electrochemical energy

58 ons in neural prosthetics, chip scale neural engineering, and extensions to different tissue and cell

59 ystallography, computer simulations, protein engineering, and functional assays to investigate the ro

60 ations for public health and for the design, engineering, and management of urban green spaces.

61 e fields of material sciences, cell biology, engineering, and many other disciplines will gradually a

62 ng nanotechnology, microfluidics, electronic engineering, and material science have boosted a new era

63 Overall, our study indicates that the engineered anti-PSMA peptide-targeted EMs can be a promi

64 em for programmable DNA insertions in genome-engineering applications.

65 Thus, the topological engineering approach enriches the toolbox for high-throu

66 production of bulk chemicals via a metabolic engineering approach it is necessary to better character

67 Here, we demonstrate a nanoscale interface-engineering approach that harnesses the large chemically

68 pportunities one can exploit using a crystal engineering approach, for example, the design of novel d

69 Using a CRISPR/Cas9-based engineering approach, we genetically deleted five large

70 r substrate binding obtained using a crystal engineering approach.

71 ms to provide an updated overview of the key engineering approaches for better exploiting EVs in dise

72 re form, highlighting the need for metabolic engineering approaches for high-level Taxol production i

73 al bacterial delivery, from internal genetic engineering approaches to external encapsulation and mod

74 Diverse efforts in protein engineering are beginning to produce novel kinds of symm

75 arallel, cellular reprogramming and organoid engineering are expanding the use of human neuronal mode

76 endolysosomal network by knocking in (KI) an engineered ascorbate peroxidase (APEX) gene to the endog

77 Heating, Refrigerating, and Air-Conditioning Engineers (ASHRAE).

78 in vivo extracellular recording and genetic-engineering-assisted optical stimulation is a powerful t

79 ry generation problems, ranging from protein engineering attempts that leverage mutual information to

80 lution of NLRs, give an overview of previous engineering attempts, and propose how to use evolutionar

81 Engineering bacteria to clean-up oil spills is rapidly a

82 The engineered bacterial strain, referred to as SYNB1891, ta

83 In food matrix studies, a cocktail of engineered bacteriophages accurately detected 1 CFU in e

84 demonstrates efficient optical metamaterial engineering based on structured ensembles of atoms(4,8,9

85 ly involves many parameters, making material engineering based on trial and error highly inefficient.

86 e an arrayed CRISPR screening method, Genome engineering-based Interrogation of Enhancers (GenIE), wh

87 t there is no loss in bioturbation ecosystem engineering behaviors after the mass extinction, and sec

88 s for molecular evolution and our ability to engineer biological systems.

89 ancer, these studies are relevant for tissue engineering, biological effects of materials, tissue and

90 vances in targeted exosomal delivery systems engineered by aptamer for future strategies to promote h

91 Defect engineering can enhance key properties of metal-organic

92 o demonstrates that efficient surface ligand engineering can exploit the unique electrochemical prope

93 Vector engineering can increase AAV transduction efficiency (by

94 rude oil in aged carbonate rocks using novel engineered carbon nanosheets (E-CNS) derived from sub-bi

95 This approach is applied to engineer cartilage constructs with a depth-dependent cel

96 biology questions otherwise intractable and engineer cell functions for future synthetic biology app

97 mal cells (hMSCs) are a promising source for engineered cell-based therapies in which genetic enginee

98 Genome stability is essential for engineering cell-based devices and reporter systems.

99 ied at the single-cell level, due largely to engineering challenges related to sample stability, heat

100 s, and to different radiant powers, by using engineered channels with different temperature threshold

101 mpared therapeutic properties of natural and engineered chemokine (C-X-C motif) receptor 4 (CXCR4) ag

102 a body part (embodiment) has been inspiring engineers, clinicians, and scientists as a means to opti

103 ll be of particular relevance to the crystal engineering community, whose goal is the design of solid

104 een given to analyzing patterns in ecosystem engineering complexity as a result of the extinction dri

105 neered cell-based therapies in which genetic engineering could enhance therapeutic efficacy and insta

106 Both an MS2-MCP system and an engineered CRISPR-Cas13 system were used to deliver APEX

107 ABCA12 using HI patient skin samples and an engineered CRISPR/Cas9 ABCA12 KO cell line.

108 s of climate change and providing a means to engineer crops for entirely novel environments, such as

109 ditions, photoreactivity, water quality, and engineering design in the sunlight inactivation of virus

110 In engineering design, incorporating certain non-Markovian

111 ttlenecks in a broad range of scientific and engineering disciplines(1,2).

112 Engineered disulfide bridges that locked the cassettes i

113 Engineered DNA substrates that stabilize a reaction inte

114 and open a new direction in precision defect engineering, down to a single defect, towards achieving

115 transposase proteins, and will guide protein engineering efforts to leverage this system for programm

116 bacterial organelles and will aid downstream engineering efforts.

117 e perform a detailed characterization of our engineered ENHANCE system with various crRNA modificatio

118 xenobiotics, like pesticides, in natural and engineered environments is poor.

119 ssible and cost-effective as compared to the engineered enzyme and click chemistry reagents.

120 algorithm called TopoBuilder, with which we engineered epitope-focused immunogens displaying complex

121 urface protection, materials and electrolyte engineering, etc.).

122 , and optogenetics that have been used to re-engineer existing molecular motors to have, for instance

123 C)-based technology with CRISPR-based genome engineering facilitates precise isogenic comparisons of

124 , and expertise from multiple biomedical and engineering fields will be needed to fully realize the p

125 We therefore engineered flies expressing a genetically encoded Ca(2+)

126 s (PIV) vector expressing RSV fusion protein engineered for enhanced immunogenicity.

127 tors in bacterial metabolism, their rational engineering for commercial metabolite production in phot

128 hich has the potential to revolutionise cell engineering for therapeutic applications.

129 Among them, Magnets are small modules engineered from the Neurospora crassa photoreceptor Vivi

130 gulated through structural and compositional engineering from the macroscale down to the nanoscale, i

131 , and 2) minimize the nND immune response by engineering fusion proteins consisting of gp120 Core and

132 e apply synthetic biology methods to reverse-engineer gene expression control in S. pneumoniae A sele

133 advances in fundamental immunology, genetic engineering, gene editing, and synthetic biology exponen

134 ular microgravity responsive device using an engineered genetic circuit in E.coli, which responded to

135 Engineered genetic systems are prone to failure when the

136 Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-Gal

137 heoretical analysis of the system, providing engineering guidelines for its design and operation.

138 The field of tissue engineering has advanced over the past decade, but the l

139 Breakthroughs in materials engineering have accelerated the progress of immunothera

140 olytic enzyme expression, rendering knockout-engineered heart tissue sensitive to metabolic stress su

141 cells and reconstitution of this genetically engineered hematopoietic system in mice.

142 nation with efficiencies that rival those of engineered hemin proteins.

143 Approaches to genetically engineer high HbF include de novo generation of naturall

144 eric serum protein and was selected here for engineering higher-valency molecules because of its comp

145 genetic studies of hMSCs and development of engineered hMSC-based therapies.

146 Genome-scale engineering holds great potential to impact science, ind

147 RBP4) of the lipocalin family interacts with engineered hRBP4 binders in a small molecule-dependent m

148 We used CRISPR-Cas9 to engineer human cell lines expressing POLE tumor variants

149 lular and behavioral outcomes in genetically engineered human APOE targeted replacement (TR) mice fol

150 acity to penetrate tumors(5), we genetically engineered human macrophages with CARs to direct their p

151 d, polymeric and inorganic nanoparticles are engineered in increasingly specified ways, they can begi

152 iology and treatment from the perspective of engineered in vitro models spanning from conventional pl

153 oping, complex, and under-recognized role of engineering in medicine to address the multitude of chal

154 ing using the Spectralis HRA+OCT (Heidelberg Engineering, Inc., Heidelberg, Germany).

155 asing primary extinctions, larger numbers of engineers increase stability by reducing primary extinct

156 osing the correct combination of targets for engineering increased fruit yield.

157 Here, we engineer inducible nitrogenase activity in two cereal en

158 ng the conjugation platform, we successfully engineer INS-1E, a beta-cell line, to repurpose the insu

159 ations using mice with picrotoxin resistance engineered into receptors containing the delta subunit.

160 Cardiac tissue engineering is a promising approach to treat cardiovascu

161 The microfluidics engineering is central to achieve a controlled functiona

162 ellular processes in tissue and regenerative engineering is now easily possible.

163 Transplantation of SA-PDL1-engineered islet grafts with a short course of rapamycin

164 e recapitulated in two sets of independently engineered isogenic N40D iNs.

165 enzymatic assays, mutant analysis, metabolic engineering, isotope labeling and metabolic profiling to

166 Finally, the developing field of engineered macrophages, including genetic engineering an

167 portant tool, but is still rarely applied to engineer mammalian cells.

168 tigue-resistant hydrogel coatings on diverse engineering materials with complex geometries.

169 ituent Mn(2+) and Ca(2+) ions in genetically engineered membranes of the cyanobacterium Synechocystis

170 depending on temperature and the site of the engineered methionine.

171 alleviate the hurdles of conventional tissue engineering methods by precise and controlled layer-by-l

172 atypical neurofibromas generated genetically engineered mice (GEM)-PNST similar to human MPNST, and t

173 oclonal antibodies by immunizing genetically engineered mice that have a full set of human immunoglob

174 Recent studies using engineered microenvironments and phagocytic targets have

175 Engineering microrobots is increasingly receiving attent

176 that a detailed understanding is required to engineer modified FA production in oilseeds and suggest

177 In a genetically engineered mouse model of non-small cell lung cancer dri

178 animals often hinders the use of genetically engineered mouse models (GEMM) in cancer research.

179 Genetically engineered mouse models (GEMMs) of cancer have proven to

180 tissue homeostasis, we generated genetically engineered mouse models where we can conditionally delet

181 addition to patient samples and genetically engineered mouse models, we developed organoid systems f

182 , transgenic, and other types of genetically engineered mouse strains.

183 In contrast, intravenously injected engineered MSCs were undetectable within grafts and lack

184 localized correction may be achievable using engineered MSCs, strategies for systemic administration

185 vestigate the effects and modes of action of engineered nanomaterials (ENMs) in this way.

186 ctions of the environmental fate and risk of engineered nanomaterials (ENMs) require a better underst

187 However, very few engineered nanoparticles can be excreted through salivar

188 efforts to perform targeted drug delivery by engineered nanoparticles have shown some success, there

189 GMT in a hydrolysable polymer structure, and engineer NPs of this polymeric chemotherapy.

190 ble metal containing natural, incidental, or engineered NPs in soil.

191 Here we report that Fc engineering of anti-influenza IgG monoclonal antibodies

192 Herein, for the first time, the engineering of C(3) N(4) layers with single-atom Cu bond

193 er of examples in the literature of targeted engineering of conformational dynamics being successfull

194 s the prospects of sigma factor in metabolic engineering of cyanobacteria, summarizes the challenges

195 to mammalian cells all at once or extensive engineering of gene regulatory sequences can be used to

196 ons(2-5), however, have greatly hampered the engineering of low-entropy molecular systems(6).

197 and tested their utility for precise somatic engineering of missense mutations in key cancer drivers.

198 rboxysomes have inspired rational design and engineering of new nanomaterials to incorporate desired

199 ties enable the rational design and holistic engineering of novel materials for more capable biocompa

200 genetic engineering of promastigotes for cytosolic accumulation

201 into an automated format for high-throughput engineering of small-molecule-binding aptamers to acquir

202 e findings suggest the possibility of strain engineering of the transport properties of BAs for appli

203 ploring MbA biosynthesis to enable metabolic engineering of this rare and valuable compound.

204 Bivalent domain-swapped Fab dimers engineered on the basis of HC84.26.5D may also serve as

205 tise but without formal training in software engineering or computer science.

206 Here, we use genetically engineered orthotopic mouse models of breast cancer to s

207 P450 variant, P411-C10, yielded a lineage of engineered P411 enzymes that together accommodate a vari

208 By re-engineering peptidoglycan synthesis, we have constructed

209 There is considerable interest in engineering plant cell wall components, particularly lig

210 ins in SA signalling and their potential for engineering plant immunity.

211 The design principles for engineering plasticity described can be applied to numer

212 This study establishes a precise genetic engineering platform for genetic studies of hMSCs and de

213 eveloping such systems becomes a commonplace engineering practice, with accepted and trustworthy deli

214 systems not only establish a set of general engineering principles which can be used to convert natu

215 n of regulatory networks as part of cellular engineering projects, whether it be to stage processes d

216 al design has emerged as a powerful means to engineer proteins, but requires detailed knowledge about

217 ductivity in TBG and open up avenues towards engineering quantum phases in moire systems.

218 While small numbers of engineers reduce stability by increasing primary extinct

219 In this Review, we summarize how engineered reproductive tissues facilitate research in r

220 biology, and overview strategies for making engineered reproductive tissues that might eventually al

221 vate a campus culture of ethical science and engineering research in the very work settings where lab

222 Genome engineering revealed that two enhancers with half EREs c

223 erform live-cell dynamic tension imaging, we engineered reversible probes with a cryptic docking site

224 C- terminus of peptides using wild-type and engineered ribosomes.

225 and have implications with regard to future engineering schemes, leading to better crop yields.

226 The vibrancy of the engineering science associated with these platforms, the

227 undamentally limited and can be broadened by engineering selective optical coupling mechanisms to the

228 re, we have combined CRISPR gene editing and engineered separation-of-function mutants to define how

229 ighted, FLAIR, and ADC images as well as two engineered sequences; T1post-T1pre and T2-FLAIR.

230 We engineered signaling aptasensors for the detection of RN

231 M204 and an engineered single-chain variable fragment (scFv) inhibit

232 Among the myriad of approaches attempted to engineer solid organs, 3D bioprinting offers unmatched p

233 th should be achieved with the transition of engineered solid organs to the clinic.

234 surface, thus can be used to create frontier engineering solutions.

235 interaction of neonatal rat heart cells with engineered spider silk protein (eADF4(C16)) tagged with

236 ile and fecund when they mate with similarly engineered strains, but incompatible with wild-type stra

237 g the current obstacles with a wide range of engineering strategies in order to improve the safety, e

238 Here, the design and engineering strategies used to develop the optimal bulk

239 Based on the engineered structure of paper materials and dried chemic

240 ions to ultrasonic sensing and evaluation of engineering structures.

241 Harnessing conformational dynamics in engineering studies is a powerful paradigm with which to

242 ri-aspartate architectures, which allows for engineering such a selective multivalent metal ion bindi

243 ule vectorial folding (VF) assay in which an engineered superhelicase Rep-X sequentially releases flu

244 Here, we combine protein engineering, surface plasmon resonance characterization,

245 ID footprints across the entire length of an engineered switch region in cells ablated for uracil rep

246 organic pollutant degradation in natural and engineered systems, such as during the remediation of co

247 control crystallization in both natural and engineered systems, which occurs in complex multicompone

248 n determining the role of DOM in natural and engineered systems, yet there is still considerable unce

249 duction, which is generally not desirable in engineered systems.

250 ants is of importance in various natural and engineered systems.

251 Adoptive transfer of engineered T cells into patients resulted in durable eng

252 Engineering T lymphocytes is an emerging approach in a v

253 Later, the spread of genetic engineering technology enabled investigators to develop

254 ltration, sensing, drug delivery, and tissue engineering that often require the fibers to be patterne

255 When countries engineer the climate, conflict can arise because differe

256 studies is a powerful paradigm with which to engineer the next generation of designer biocatalysts.

257 a ligand-assisted surface matrix strategy to engineer the surface and packing states of Pe-QD solids

258 Here we engineered the spike D614G substitution in the USA-WA1/2

259 We have thus developed avenues for engineering the surface of nanoparticles for biological

260 ical biogeography of an important ecological engineer: the dusky-footed woodrat, Neotoma fuscipes.

261 lgal CCMs, as well as recent advances toward engineering these components into land plants.

262 erein, a series of oxytocin derivatives were engineered through conjugation with fatty acid moieties

263 ted in this work can quantitatively evaluate engineered tissues and contribute to a robust understand

264 r patterning perfusable vascular networks in engineered tissues have been constrained in architectura

265 into morphogen evolution and a platform for engineering tissues.

266 rom proteases, and these interactions can be engineered to be reversible and optically controlled.

267 Using a LukGH variant engineered to bind mouse CD11b-I, we demonstrate that cy

268 Two different protein scaffolds were engineered to bind to hRBP4 when loaded with the orally

269 tro self-renewal ability, and can be readily engineered to enhance their immunomodulatory functions.

270 Here, we demonstrate that NK cells (haNKs) engineered to express a PD-L1 chimeric antigen receptor

271 T cells engineered to express antigen-specific T cell receptors

272 Both XplA and BezE could be engineered to further improve their C-H amination reacti

273 led microengines to hybrid spermbots, can be engineered to integrate sophisticated features optimised

274 Using a strain of congenic SAMP mice engineered to lack global expression of ERbeta, we obser

275 gakaryocyte progenitor cells are genetically engineered to overexpress PD-L1 to produce immunosuppres

276 threatens the benefits of crops genetically engineered to produce insecticidal proteins from Bacillu

277 o identify a missing pathway enzyme, protein engineering to enable the functional expression of an ac

278 confinement effects facilitate wave function engineering to sculpt the spatial distribution of charge

279 specificity and kinetics of Cas9 as a genome engineering tool and may inspire expanded applications t

280 rful tools for greatly expanding the protein engineering toolkit.

281 gold nanorods bound to temperature-sensitive engineered transient receptor potential (TRP) channels.

282 sis process, activate hIL-10 receptors in an engineered U2OS osteosarcoma cell line, and increase cel

283 Engineered ureteric buds branched in three-dimensional c

284 To explore these mechanisms, we engineered V5-FOXL2(WT)- and V5-FOXL2(C134W)-inducible i

285 engaging FcgammaRs, and demonstrate that Fc-engineered versions of the mAb could be used to reduce s

286 our group demonstrated that acellular tissue engineered vessels (A-TEVs) comprised of small intestina

287 e and transport of pollutants in natural and engineered water systems.

288 Last, we find that engineered waterways, whose emissions are currently not

289 r lipid bilayers in conjunction with protein engineering, we explicate the mechanism by which the int

290 In contrast to a top-down method of tissue engineering where the differentiation of cells is guided

291 A hybrid PKS module harboring KR1 was engineered, whose individual catalytic domains have been

292 leractinian "stony" corals are major habitat engineers, whose skeletons form the framework for the hi

293 e cause of this deafness using a mouse model engineered with a noncoding intronic 10 bp deletion (del

294 The paper-based sensor was then engineered with a novel and highly specific peptide nucl

295 Engineering with biomolecular motors has the potential t

296 scope of possibilities for colloidal crystal engineering with DNA.

297 tegic selection of substrate cells, and gene-engineering with synthetic co-stimulatory circuits.

298 hypothesized that ML differentiation and CAR engineering would result in complementary improvements i

299 Using deep mutational scanning, we engineered yeast with all 44,604 single codon changes en

300 be similar to the amplification produced by engineered zinc nanoparticles.