ライフサイエンスコーパス: enhancer trap

1 eakpoints, and by the expression patterns of enhancer traps.

2 ry system for poplar trees based on gene and enhancer traps.

3 be more effective for activation tagging and enhancer trapping.

4 ranscription in Arabidopsis, we used in vivo enhancer trapping.

5 trate that lacZ expression from the dpp(F11) enhancer trap accurately reflects dpp mRNA accumulation

6 Enhancer trap analysis of the smi lines indicates that e

7 Using enhancer trap and Cre transgenes, our lineage tracing ex

8 knockdown, expression-based screening using enhancer trapping and forward genetic screening using tr

9 ial chromosomes (BACs) were retrofitted with enhancer traps, and expressed as transgenes in zebrafish

10 as insertional mutagenesis screens, gene and enhancer trap applications, and gene therapy.

11 The results show that the enhancer trap approach can be a productive way of explor

12 We have taken an enhancer trap approach to identify genes that are expres

13 We used an enhancer trap approach to study the adult structure, con

14 Here we report on the utilization of enhancer trap approach toward the identification and ana

15 Using the enhancer trapping approach, we identified a new Drosophi

16 We have used an enhancer-trap approach to begin characterizing the funct

17 e confirmed that the genes detected by these enhancer traps are expressed in patterns similar to thos

18 To address this issue, we devised an enhancer trap assay to systematically screen 1 Mb of DNA

19 Using an enhancer trapping assay, we discovered a novel osmosensi

20 A gal4-containing enhancer-trap called C309 was previously shown to cause

21 Our T-DNA enhancer trapping cassette consisted of two principle co

22 We demonstrate the utility of this enhancer trap-compatible split luciferase assay (ETSLA)

23 aused by the insertion of a DsE transposable enhancer trap element into the 5' untranslated leader of

24 Finally, I have mapped an enhancer trap element within the 5' region of dFKBP59 wh

25 Here, we report that using an enhancer-trap embryo mutant of Arabidopsis, we identifie

26 Individual lines identified by enhancer trapping exhibit peak transcription rates at ci

27 This phenotypic enhancer-trap facilitates (i) the isolation of enhancer-

28 intersectional approach with our library of enhancer trap flp-recombinase lines.

29 An enhancer-trap GAL4 line C309 directing shi(ts1) expressi

30 s, we use the MARCM techniques with a set of enhancer-trap GAL4 lines to perform systematical lineage

31 Transgenic Arabidopsis with enhancer-trapped GAL4 expression in specific cell types

32 Finally, an enhancer trap in the sr gene detects the response to Spi

33 dingly, we demonstrate the utility of ZB for enhancer trapping in zebrafish embryos and in the mouse

34 contrast to the above model, we find that an enhancer trap inserted near the Dpp target gene, Daughte

35 We screened 1300 lethal P-element enhancer trap insertions on the second chromosome for a

36 pulations because many of the genes and Gal4 enhancer trap insertions that are expressed in the ovary

37 Enhancer trap is a powerful approach for identifying tis

38 ubiquitously expressed, suggesting that the enhancer trap is detecting only a subset of its control

39 However, the gene identified by this enhancer trap is ubiquitously expressed, suggesting that

40 Using a GAL4 enhancer trap line (201Y) that drives MB-restricted repo

41 Enhancer trap line 5580 exhibits expression in young veg

42 s cloned from an Arabidopsis leaf senescence enhancer trap line and functionally characterized.

43 An enhancer trap line built by genome editing marked Tc-fox

44 w class of vascular-expressed gene tagged by enhancer trap line cET-1-pop1-145.

45 nt located at chromosomal location 33AB, the enhancer trap line E8-2-46, indicating that a gene near

46 the third segment of the antenna of the 2216 enhancer trap line in Drosophila melanogaster reveals tw

47 sociated genes, we identified an Arabidopsis enhancer trap line in which the reporter gene GUS is up-

48 nidase (GUS) reporter gene expression in the enhancer trap line is observed specifically in all cell

49 ounder cell marker Duf-LacZ, produced by the enhancer trap line rP298LacZ, is coexpressed in numerous

50 hase gene (CS) using an Arabidopsis thaliana enhancer trap line specific to veins.

51 We also identified an enhancer trap line that exhibits altered morphogenetic f

52 e was isolated by plasmid rescue from a GAL4 enhancer trap line which shows reporter gene expression

53 inally, we have molecularly characterized an enhancer trap line, AE33, that was identified in earlier

54 An enhancer trap line, line 5580, contains a T-DNA insertio

55 Starting from the H214 enhancer trap line, we identified a transcription unit,

56 tive chlorophyllase in a guard cell specific enhancer trap line.

57 cell-specific GAL4-green fluorescent protein enhancer trap line.

58 ctors regulate the expression of bft and the enhancer trap line.

59 hat lie within the expression pattern of the enhancer-trap line c929-Gal4.

60 We have used a GAL4 enhancer-trap line driving the expression of a lacZ cons

61 g method and have screened 1,300 Arabidopsis enhancer trap lines and have identified 147 lines in whi

62 Gene and enhancer trap lines defining genes expressed during prim

63 We found that 36% of our enhancer trap lines display circadian-regulated transcri

64 In contrast, the expression pattern of the enhancer trap lines during zygotic embryogenesis was mor

65 ify the R2/R5 neuronal sub-type, we screened enhancer trap lines expressed in developing photorecepto

66 ved in follicle cell patterning, we analyzed enhancer trap lines expressed in specific subsets of fol

67 elected a set of 21 frequently used GAL4/UAS enhancer trap lines for detailed characterization of exp

68 lements and use this to produce a library of enhancer trap lines for Marchantia.

69 We use GAL4 transactivation of aequorin in enhancer trap lines of Arabidopsis (Arabidopsis thaliana

70 Using enhancer trap lines targeted to different subsets of Joh

71 ectivity and provides a set of characterized enhancer trap lines that will be valuable for future stu

72 nking genomic sequence was identified for 23 enhancer trap lines to identify clock-controlled genes (

73 We produced more than 13 000 enhancer trap lines with this cassette and screened T(0)

74 ression patterns were determined for two GFP enhancer trap lines with tissue-specific expression in t

75 al circuits using genetic tools such as GAL4 enhancer trap lines, as Drosophila has been intensively

76 terized by localizing beta-gal expression in enhancer trap lines, as well as with BrdU incorporation

77 lso cloned three SAGs from randomly selected enhancer trap lines, demonstrating that reporter express

78 putative midline-expressed genes, including enhancer trap lines, microarray data, published accounts

79 By using enhancer trap lines, we find that the overproliferating

80 o either muscles or motorneurons using Gal-4 enhancer trap lines, we were able to rescue substantiall

81 DN-cadherin and Echinoid, and a set of seven enhancer trap lines.

82 l specificity is achieved through the use of enhancer trap lines.

83 cell identities is a collection of GAL4/UAS enhancer trap lines.

84 ype-specific marker lines and for genotyping enhancer trap lines.

85 We show that enhancer-trap lines can transactivate an unlinked pOp-gr

86 abidopsis research, beta-glucuronidase (GUS) enhancer-trap lines have been created and successfully u

87 demonstrate its utility by transforming GAL4 enhancer-trap lines into tissue-specific Cas9-expressing

88 This library of the Arabidopsis seed enhancer-trap lines is an efficient tool for identifying

89 ression studies, we describe a population of enhancer-trap lines obtained with LhG4AtO in a pOp-GUS b

90 Etl4(lacZ) (Enhancer trap locus 4) and Skt(Gt) (Sickle tail) are lac

91 First, we describe an enhancer trap marker called R32 that specifically reveal

92 ompanied by expression of a different set of enhancer trap markers.

93 We created a fluorescent enhancer trap marking expression of Tc-fez/earmuff, a ke

94 We previously reported that the enhancer trap Mlcv1v-nLacZ-24 transgenic mouse strain fu

95 An enhancer-trap P element was constructed with the homing

96 heterologous beta-globin gene promoter in an enhancer trap plasmid and of the alpha1(I) collagen gene

97 Characterization of an enhancer-trap population identified multiple lines that

98 We identified an enhancer trap probe often producing restricted reporter

99 Poplar gene and enhancer traps provide a new resource that allows plant

100 method adds two components: a collection of enhancer-trap recombinase, Flippase (ET-FLP), transgenic

101 DPP induces expression of the enhancer trap reporter A359 and represses expression of

102 One enhancer trap revealed expression of the gene encoding t

103 fat body-specific green fluorescent protein enhancer trap screen in D. melanogaster and expression p

104 A gene identified from an enhancer trap screen is shown to encode the Drosophila m

105 An enhancer trap screen of synapse-inactivated neural circu

106 sis of events in the margin, we performed an enhancer trap screen to identify genes specifically expr

107 We present a pilot enhancer trap screen using GAL4 to drive expression of u

108 xpression system, we performed a large-scale enhancer-trap screen for insertions that yielded nervous

109 reporter expression and developed efficient enhancer trap screening with the PiggyBac transposon.

110 gene of Drosophila melanogaster, defined by enhancer trap strain 24B of Brand and Perrimon.

111 Using an enhancer trap strain in which white+ gene expression is

112 using a set of Drosophila melanogaster GAL4 enhancer trap strains for an upper-level undergraduate l

113 P-element-based gene and enhancer trap strategies have provided a wealth of infor

114 n vivo bacterial artificial chromosome (BAC) enhancer-trapping strategy in mice to scan a half-megaba

115 This enhancer trap system presents a useful resource for the

116 e split luciferase construct with a GAL4 GFP enhancer trap system, we describe a tissue-specific spli

117 The Tol2-mediated gene/enhancer trapping system together with UAS transgenic li

118 n generated using a GAL4-dependent bipartite enhancer-trap system confirmed that PPhiB or phytochrome

119 H-2Z1 is an enhancer trap transgenic mouse line in which the lacZ re

120 A collection of Arabidopsis enhancer trap transposants has been identified for use a

121 ant allele caused by the insertion of a GAL4 enhancer trap transposon.

122 In that study, we mobilized a hobo enhancer trap vector and generated two unique alleles of

123 n system to distribute a self-reporting gene/enhancer trap vector efficiently throughout the zebrafis

124 Using a self-reporting Gal4-VP16 gene/enhancer trap vector, we isolated tissue-specific driver

125 When incorporated into an enhancer-trapping vector, however, LhG4AtO and Lh314 yie

126 Gene and enhancer trap vectors carrying the beta-glucuronidase (G

127 specific ectopic expression of the A251-lacZ enhancer trap whereas overexpression of a transgene enco

128 We used enhancer trapping with the GAL4 transcriptional activato

129 g vector, however, LhG4AtO and Lh314 yielded enhancer traps with approximately twice the frequency of

130 hancer-trap facilitates (i) the isolation of enhancer-traps with a specific expression pattern, and (