ライフサイエンスコーパス: enoyl-CoA hydratase

1 larly important to the catalytic activity of enoyl-CoA hydratase.

2 Glu139 are required for the high kcat of the enoyl-CoA hydratase.

3 i was identified as the catalytic residue of enoyl-CoA hydratase.

4 A ligase, and RpfF shows some relatedness to enoyl CoA hydratases.

5 ., E269 in aldehyde dehydrogenase-1; D204 in enoyl CoA hydratase-1), as well as residues of unknown f

6 thors show that exceeding nutrients suppress Enoyl-CoA hydratase-1 (ECHS1) activity by inducing its a

7 Herein, we report that enoyl-CoA hydratase-1 (ECHS1), the enzyme involved in th

8 Aconitase-2 and enoyl-CoA-hydratase-1 expression levels were decreased i

9 Depletion of aconitase-2 and enoyl-CoA-hydratase-1 resulted in the inhibition of the

10 selective depletion of both aconitase-2 and enoyl-CoA-hydratase-1.

11 hat may contribute to renal function include enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (EHH

12 l 3-oxoacyl-CoA thiolase (THIO), peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (HD)

13 hree enzymes: fatty acyl-CoA oxidase (ACOX), enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (HD)

14 A weak enoyl-CoA hydratase activity was detected for both DpgB

15 lutaryl-CoA dehydrogenase also has intrinsic enoyl-CoA hydratase activity, a property of other member

16 ectra have been obtained for HD-CoA bound to enoyl-CoA hydratase, an enzyme system that has also prev

17 y and show another stable complex, PaaFG, an enoyl-CoA hydratase and enoyl-Coa isomerase, both belong

18 hexadienoyl-CoA (HD-CoA), bound to wild-type enoyl-CoA hydratase and G141P, a mutant in which a hydro

19 tyl-CoA when bound as an enolate to MCAD and enoyl-CoA hydratase and is used to rationalize the obser

20 ur separate reactions, two of which (2-trans enoyl-CoA hydratase and L-3-hydroxyacyl-CoA dehydrogenas

21 o contains the active site of long-chain 2,3-enoyl-CoA hydratase and long-chain 3-ketoacyl-CoA thiola

22 lytic properties of 3-ketoacyl-CoA thiolase, enoyl-CoA hydratase, and delta 3-cis-delta 2-trans-enoyl

23 Enoyl-CoA hydratase catalyzes the hydration of trans-2-c

24 Significantly, binding to enoyl-CoA hydratase causes the chemical shifts of the C1

25 th members of the crotonase family including enoyl-CoA hydratase (crotonase) and methylmalonyl-CoA de

26 Members of the enoyl-CoA hydratase (crotonase) superfamily catalyze dif

27 Gene deletion analyses confirmed that the enoyl-CoA hydratase/dehydrogenase Fox2p, the putative 3-

28 Enoyl-CoA hydratase domain-containing 3 (ECHDC3) was the

29 termined the crystal structure of the enzyme enoyl-CoA hydratase (ECH) from rat liver with the bound

30 enecyclopropyl)glycine, against bovine liver enoyl-CoA hydratase (ECH) were characterized.

31 TFTH-CoA can be directly bioactivated by the enoyl-CoA hydratase (ECH) with the release of 1,2-dichlo

32 n of long chain fatty acyl-CoAs, employing 2-enoyl-CoA hydratase (ECH), 3-hydroxyl-CoA dehydrogenase

33 ium (PDB ID 3q1t) has been reported to be an enoyl-CoA hydratase (ECH), but SALSA analysis shows a po

34 ve site-directed inactivator of bovine liver enoyl-CoA hydratase (ECH).

35 Such auxiliary enzymes, including the enoyl-CoA hydratase ECH2, convert (R)-3-hydroxyacyl-CoA

36 ation of crotonyl-CoA suggesting short chain enoyl-CoA hydratase (ECHS1) activity was inhibited.

37 s that inhibited the activity of short chain enoyl-CoA hydratase (ECHS1).

38 ditions for crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and

39 ditions for crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and

40 to trichome specific acyl-CoA synthetase and enoyl-CoA hydratase genes.

41 noyl-coenzyme A (HD-CoA) bound to the enzyme enoyl-CoA hydratase has been determined using transferre

42 sidues (E164 and E144) in the active site of enoyl-CoA hydratase has been probed by site-directed mut

43 e promoter of the PPARalpha target genes rat enoyl-CoA hydratase (HD) and peroxisomal fatty acyl-CoA

44 ) with upregulation of ech-1.1 (a homolog of enoyl-CoA hydratase involved in fatty acid beta-oxidatio

45 These data suggest that enoyl-CoA hydratase is an important enzyme in the bioact

46 rases but showed significant homology to the enoyl-CoA hydratase/isomerase enzyme family.

47 athways associated with the members of the 2-enoyl-CoA hydratase/isomerase enzyme superfamily are com

48 esis, the active site of one member of the 2-enoyl-CoA hydratase/isomerase family, 4-chlorobenzoyl-Co

49 erases and a C-terminal module homologous to enoyl-CoA hydratases/isomerases.

50 se/reductase family and that IBR10 resembles enoyl-CoA hydratases/isomerases.

51 oxidation at the level of the second enzyme, enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase (L

52 In this study, the function of enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase (L

53 ex enhanced transcription from a peroxisomal enoyl-CoA hydratase/l-3-hydroxyacyl-CoA dehydrogenase bi

54 especially of CBP and TRAP150, to the mouse enoyl-CoA hydratase/l-3-hydroxyacyl-CoA dehydrogenase ge

55 urated substrates bind to the active site of enoyl-CoA hydratase, large spectral changes can be obser

56 of the component enzymes of TOC, long-chain enoyl-CoA hydratase, long-chain 3-hydroxyacyl-CoA dehydr

57 somal beta-oxidation mutants showed that the enoyl CoA-hydratase MAOC-1 serves an important role in a

58 We report a previously unassigned modular enoyl-CoA hydratase (mECH) domain and the assembly of en

59 In contrast, growth of a putative enoyl-CoA hydratase mutant (DeltaechA) was abolished on

60 eding experiments show that the MFP2 2-trans enoyl-CoA hydratase only exhibits activity against long

61 of the buffer effect on the mechanism of the enoyl-CoA hydratase reaction is discussed.

62 ng the expression of the gene ech-6 encoding enoyl-CoA hydratase remitted fat diet-induced deleteriou

63 n genes trxA (Rv1470), trxB (Rv1471), and an enoyl-coA hydratase (Rv1472), indicating a possible role

64 downregulation of the crotonyl-CoA hydratase enoyl-CoA hydratase short chain 1 (ECHS1), leading to ac

65 ed enzymes, the acyl-CoA ligase SidI and the enoyl-CoA hydratase SidH, linking biosynthesis of mevalo

66 uence homology validates the modeling of the enoyl-CoA hydratase structure with the 4-(chlorobenzoyl)

67 G141 is part of a consensus sequence in the enoyl-CoA hydratase superfamily, the results presented h

68 e encodes seven paralogues of the crotonase (enoyl CoA hydratase) superfamily.

69 coupled with the strong sequence homology to enoyl-CoA hydratase support the intramolecular suprafaci

70 wo conserved glutamate residues of rat liver enoyl-CoA hydratase to which Glu119 and Glu139 of the la

71 hat is catalytically essential in homologous enoyl-CoA hydratases was also essential in CHY1.

72 -fold decrease, respectively, in the kcat of enoyl-CoA hydratase without a significant change in the