ライフサイエンスコーパス: enzymatic digestion

1 off line or in situ proteomic methods using enzymatic digestion.

2 lated from normal human term placentas using enzymatic digestion.

3 demineralized mouse periodontal sections via enzymatic digestion.

4 the peptide C-terminal carboxyl group during enzymatic digestion.

5 chymal cells were isolated by mechanical and enzymatic digestion.

6 by LC-MS analysis of peptides obtained from enzymatic digestion.

7 reference RNA, is labeled with (16)O during enzymatic digestion.

8 ctance Ca(2+)-sensitive K(+) channels and to enzymatic digestion.

9 stroyed during strong acid digestion but not enzymatic digestion.

10 o monitor peptide products after an on-probe enzymatic digestion.

11 y SDS-PAGE, excised, and subjected to in-gel enzymatic digestion.

12 mammalian RNA by centrifugation, followed by enzymatic digestion.

13 e bovine NPE cells were freshly harvested by enzymatic digestion.

14 ized before and after high pressure-assisted enzymatic digestion.

15 lonic circular muscle cells were obtained by enzymatic digestion.

16 by a high Ca(2+) concentration, even during enzymatic digestion.

17 ion of modified proteins without recourse to enzymatic digestion.

18 s are produced that are resistant to further enzymatic digestion.

19 A genes, large-scale plasmid preparation and enzymatic digestion.

20 Muscle cells were obtained by enzymatic digestion.

21 cidated using MALDI-MS coupled with on-plate enzymatic digestion.

22 Gallbladder muscle cells were obtained by enzymatic digestion.

23 ere characterized by NMR spectroscopy and by enzymatic digestion.

24 mical accessibility and fragment order after enzymatic digestion.

25 lexes also diminished significantly with the enzymatic digestion.

26 reated controls using collagenase-A-assisted enzymatic digestion.

27 nly performed using surrogate peptides after enzymatic digestion.

28 ions that contribute to its recalcitrance to enzymatic digestion.

29 in a disulfide mapping application involving enzymatic digestion.

30 ified NAA-PFs (i.e., sensing element) during enzymatic digestion.

31 vers of patients and mice via mechanical and enzymatic digestion.

32 glycopeptides resulting from highly specific enzymatic digestions.

33 resonance (NMR), combined with chemical and enzymatic digestions.

34 ntitative disaccharide profiles, and partial enzymatic digestions.

35 brane segment using a series of chemical and enzymatic digestions.

36 rosequencing and mass spectrometry following enzymatic digestions.

37 nd gel-filtration, and subjected to multiple enzymatic digestions.

38 facilitates robust protein extraction, rapid enzymatic digestion (30 min compared to overnight), and

39 ycan moieties from cell surface antigens via enzymatic digestion, a process termed sample deglycosyla

40 strategy, which involved labeling reaction, enzymatic digestion, affinity purification, and LC-MS/MS

41 roup onto the N-termini of peptides, and the enzymatic digestion after the derivatization step remove

42 s released from amaranth seed proteins after enzymatic digestion, against dipeptidyl peptidase IV (DP

43 ion strategies such as hyperbranching and/or enzymatic digestion/amplification.

44 Cells prepared from muscle by enzymatic digestion and 5-day in vitro culture were harv

45 residues provides a potential alternative to enzymatic digestion and a possibility for further chemic

46 ted from the large-diameter core fiber after enzymatic digestion and acid hydrolysis demonstrated the

47 Methods involving enzymatic digestion and chemical depolymerization have b

48 -100 kDa) obtained after different levels of enzymatic digestion and chemical reduction.

49 Comparison between enzymatic digestion and conventional homogenization tiss

50 ril formation, more prone to disaggregation, enzymatic digestion and degradation by the 26S proteasom

51 timization and several key parameters, i.e., enzymatic digestion and derivatization, in the assay dev

52 on-digestible fractions (NDF) produced after enzymatic digestion and determine their antiproliferativ

53 e purified from excised pancreatic tissue by enzymatic digestion and discontinuous density gradient p

54 these formations were isolated by selective enzymatic digestion and further purified on the basis of

55 A method using a combination of enzymatic digestion and ionspray mass spectrometry (MS)

56 toxins) were enriched and purified prior to enzymatic digestion and LC-MS analysis.

57 ivatives of T, C, A, and G, as identified by enzymatic digestion and LC-MS/MS.

58 y 3'-overhanging ends, were characterized by enzymatic digestion and MALDI-TOF mass spectrometry.

59 s were purified by HPLC and characterized by enzymatic digestion and MALDI-TOF mass spectrometry.

60 Chemical cross-linking combined with an enzymatic digestion and mass spectrometric analysis of t

61 complete, including all chemical reactions, enzymatic digestion and mass spectrometry experiments.

62 A method using a combination of exonuclease enzymatic digestion and matrix-assisted laser desorption

63 Enzymatic digestion and mutational analysis identified n

64 Enzymatic digestion and peptide identification using tan

65 Single cells were isolated by enzymatic digestion and permeabilized by saponin.

66 protein, which poses several challenges for enzymatic digestion and protein identification.

67 physiological gp17/SABP protein by coupling enzymatic digestion and ProteinChip technology.

68 gy--a process of "self-eating" that involves enzymatic digestion and recycling of cellular constituen

69 y - a process of "self-eating" that involves enzymatic digestion and recycling of cellular constituen

70 Here we report results from the analyses by enzymatic digestion and reversed-phase ion-pairing liqui

71 traditional procedures involving in-solution enzymatic digestion and sample cleanup prior to MALDI-MS

72 When this method is combined with limited enzymatic digestion and tandem mass spectrometric techni

73 ytes were obtained from knee cartilage after enzymatic digestion and treated with IL-1beta in the abs

74 Cardiomyocytes were isolated using enzymatic digestion and were studied under varying degre

75 hanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation.

76 By computing the optimal enzymatic digestions and size selection steps required,

77 Enzymatic digestions and subsequent analysis confirmed t

78 This method is also applicable to other enzymatic digestions and used to monitor site-specific d

79 ization, i.e., a 15 min immunocapture, a 2 h enzymatic digestion, and an UPLC-MS/MS detection, allowe

80 zed by absorption and emission spectroscopy, enzymatic digestion, and electrophoresis, form stable du

81 F MS and indirectly by subsequent reduction, enzymatic digestion, and GC/MS.

82 ed a soluble form by chemical fragmentation, enzymatic digestion, and mass spectrometry and a full-le

83 icrochip electrophoresis, standard addition, enzymatic digestion, and matrix-assisted laser desorptio

84 ith a combination of biochemical enrichment, enzymatic digestion, and nano-scale liquid chromatograph

85 eled with [(3)H]tetracaine were subjected to enzymatic digestion, and peptides containing (3)H were p

86 photolabeled with [3H]dTC were subjected to enzymatic digestion, and peptides containing 3H were iso

87 ally subjected to selective and nonselective enzymatic digestion, and the isomeric products from each

88 f a broader range of particle sizes than the enzymatic digestion approach, highlighting the need for

89 ionization mass spectrometry (MS) along with enzymatic digestion as a tool to examine at near residue

90 The feasibility of enzymatic digestion as an alternate tissue preparation t

91 ge via the latter pathway is confirmed by an enzymatic digestion assay, verifying the reliability of

92 Using a combination of enzymatic digestion assays coupled with gel electrophore

93 Enzymatic digestion assays in conjunction with liquid ch

94 -purified MIC2 and MS/MS analysis along with enzymatic digestion assays, we observed that at least se

95 In addition, the enzymatic digestion at the Lys28 loop region linking the

96 were isolated from adult zebrafish hearts by enzymatic digestion, attached to carbon rods, and twitch

97 ides an efficient alternative to traditional enzymatic digestion-based methods for virus identificati

98 measured a signature peptide generated from enzymatic digestion, but had a lower quantitation limit

99 ctrophoresis, and electroelution followed by enzymatic digestion by both trypsin and proteinase K.

100 Enzymatic digestion by exonuclease III of the nucleosome

101 Our findings illustrate that enzymatic digestion can aid the detection of microplasti

102 Our results suggest that both dilation and enzymatic digestion can be incorporated into CED protoco

103 asured by quantifying protection of DNA from enzymatic digestion, can regulate accessibility.

104 Chemical derivatization of lysines prior to enzymatic digestion circumvents these problems and has p

105 Although the strategic use of enzymatic digestion combined with isotope dilution mass

106 ometry, circular dichroism spectroscopy, and enzymatic digestion comparative studies to investigate t

107 were generated from monoclonal antibodies by enzymatic digestion, conjugated to DOTA, and labeled wit

108 was generated from a monoclonal antibody by enzymatic digestion, conjugated to DOTA, and labeled wit

109 e of an Ibe10R fragment generated by limited enzymatic digestion did not reveal homology to any other

110 Due to the potential variability in enzymatic digestion efficiencies, the liberation of a ma

111 organic solvents, and two chaotropes on the enzymatic digestion efficiency of membrane protein-enric

112 After eggshell removal by enzymatic digestion, embryo cells are dissociated and pl

113 y described herein utilizes a combination of enzymatic digestion, ESI-MS, and MS(n)() for the sequenc

114 cle cells are purified from whole ovaries by enzymatic digestion, filtration, and fluorescence-activa

115 The procedure consists of a bleach wash, enzymatic digestion, first fixation, 'cracking', second

116 uch wall after staining with Giemsa or after enzymatic digestion followed by fluorescence-activated c

117 ocess, including the on-plate enrichment and enzymatic digestion followed by MALDI-MS analysis, can b

118 cluding disulfide reduction, alkylation, and enzymatic digestion, followed by cocrystallization with

119 olysaccharide from a bacterial spore sample, enzymatic digestion, followed by electrospray ionization

120 ation of biotin-containing nucleotides by an enzymatic digestion, followed by resolution via analytic

121 Cs from adult mice using collagenase I-based enzymatic digestion, followed by sequential sorting with

122 terminant of the recalcitrance of biomass to enzymatic digestion for biofuels production.

123 An alternative method to enzymatic digestion for protein identification by mass s

124 Enzymatic digestion for protein sequencing usually requi

125 s through microwave-assisted methanolysis or enzymatic digestion for subsequent MALDI-TOF MS analysis

126 Chondrocytes were isolated by sequential enzymatic digestion from normal ankle cartilage of organ

127 re of individual chromaffin cells derived by enzymatic digestion from the adrenal medulla of the bovi

128 DPSC and TGNC were harvested by enzymatic digestion from Wister-Hann rats.

129 Enzymatic digestion generates disaccharides with Delta-4

130 Removal of cell surface GAGs by enzymatic digestion greatly reduced the ability of mAb 4

131 Results showed that enzymatic digestion has extraction efficiency comparable

132 proteome based on the combination of global enzymatic digestion, high-resolution liquid chromatograp

133 ust (i) incorporate an isotopic label during enzymatic digestion; (ii) have no m/z interferences from

134 Enzymatic digestion in an organic-aqueous solvent system

135 nd rapid reduction of disulfide bonds before enzymatic digestion in order to increase sequence covera

136 edures, including thrombin injection, online enzymatic digestion in the microreaction system, and lab

137 particles were also remarkably stable toward enzymatic digestion in vitro and displayed long half-liv

138 However, mCyp c 1 was more sensitive to enzymatic digestion in vitro than nCyp c 1.

139 nization tissue preparation, indicating that enzymatic digestion is an appropriate tissue sample prep

140 However, enzymatic digestion is associated with additional sample

141 Enzymatic digestion is commonly used for tissue dissocia

142 recalcitrance to both acid pretreatment and enzymatic digestion is directly proportional to lignin c

143 On-tissue enzymatic digestion is performed in mass spectrometry im

144 oped a microfluidic flow cell where stepwise enzymatic digestion is performed on immobilized proteoli

145 ein denaturation, reduction, alkylation, and enzymatic digestion, is described.

146 ed by using ion trap mass spectrometry (MS), enzymatic digestion, lectin affinity chromatography, and

147 g the combination of photoaffinity labeling, enzymatic digestion, MALDI-TOF and LC-MS mass spectromet

148 port the use of a recently developed coupled enzymatic digestion/mass spectrometry assay to identify

149 An enzymatic digestion method, followed by single-particle

150 Here, we utilize an optimized enzymatic digestion methodology, previously developed fo

151 Various enzymatic digestion methods have also been developed for

152 te the possibility of replacing conventional enzymatic digestion methods with a polymer microfluidic

153 pecies characterization [by chloroquine-AGE, enzymatic digestion, multi-angle laser light-scattering

154 ort a mass spectrometry (MS) method engaging enzymatic digestion, nanoelectrospray ionization (nano-E

155 peptides needed to be selected carefully and enzymatic digestion needed to be optimized to ensure com

156 The product of the enzymatic digestion of 3H-choline-labelled cell walls wa

157 mass spectrometry (MS/MS) data begin with an enzymatic digestion of a complex protein mixture to gene

158 ion of Pseudo MS(n) to peptides generated by enzymatic digestion of a single phosphoprotein.

159 ingle V-containing fragments obtained by the enzymatic digestion of apo(a) and also kringle V-contain

160 Ammonia, the product of the enzymatic digestion of Arg by ADI, reacts with OPA and f

161 nases, as measured by gelatin zymography and enzymatic digestion of Azocoll substrates by gland lysat

162 Enzymatic digestion of beta-GlcCer and a chromatographic

163 After enzymatic digestion of BipPt-G3 duplexes, the conformati

164 btain rate curves and rate constants for the enzymatic digestion of both modified and unmodified olig

165 HCEs were acquired by enzymatic digestion of cadaveric conjunctival tissues.

166 Chondrocytes isolated by enzymatic digestion of cartilage obtained from subjects

167 Experiments using selective enzymatic digestion of cell adhesion molecules and neutr

168 Furthermore, enzymatic digestion of cell surface GAGs by heparin lyas

169 Enzymatic digestion of cell-associated HSPGs on human BM

170 ne the extent to which lignin influences the enzymatic digestion of cellulose, specifically in second

171 Monodispersed cell suspensions obtained by enzymatic digestion of conjunctival tissue were applied

172 the authentic cross-link remnant released by enzymatic digestion of cross-linked DNA.

173 Currently, enzymatic digestion of CSPGs with locally applied chondr

174 as a mixture of tryptic peptides obtained by enzymatic digestion of cytochrome c.

175 nucleoside cross-link "remnant" released by enzymatic digestion of DNA duplexes containing the dG-AP

176 It consists of four principal steps: (i) enzymatic digestion of DNA to nucleoside 3'-monophosphat

177 ciently complex with DNA and protect it from enzymatic digestion of DNase 1.

178 he nucleoside cross-link remnant released by enzymatic digestion of duplex DNA containing the dA-Ap c

179 he authentic cross-link remnants released by enzymatic digestion of duplexes containing the native an

180 complex peptide mixtures resulting from the enzymatic digestion of extracted proteins.

181 ple-transfer steps and facilitate the direct enzymatic digestion of extracted RNA within the sample c

182 cardial inflammation by flow cytometry after enzymatic digestion of hearts to characterize the infilt

183 Enzymatic digestion of IgG4.1 to the F(ab')(2)4.1 fragme

184 h based on flow-stretched DNA to monitor the enzymatic digestion of lambda-phage DNA by individual ba

185 Enzymatic digestion of major structural constituent coll

186 tion by second dimension electrophoresis and enzymatic digestion of matrix-embedded YAC DNA to produc

187 However, the enzymatic digestion of native plant cell walls is ineffi

188 ide fragment produced in our laboratory from enzymatic digestion of native protamine.

189 reduced to mainly monomers (<100 kDa) after enzymatic digestion of nucleic acids, whereas higher-ord

190 On-tissue enzymatic digestion of nude mouse brain tissue permitted

191 the host against pathogens and facilitating enzymatic digestion of other ingested bacteria.

192 Enzymatic digestion of PD with 1,4-alpha-d-polygalacturo

193 Enzymatic digestion of PNNs in the DCN improves EBC lear

194 Enzymatic digestion of polypeptides using Pronase E, a p

195 nd beta-casein, and compare the results with enzymatic digestion of proteins by trypsin.

196 emonstrate that PCT provides more controlled enzymatic digestion of proteins than prolonged digestion

197 coupled to mass spectrometry (HXMS) utilizes enzymatic digestion of proteins to localize the informat

198 chaotropes and detergents) known to improve enzymatic digestion of proteins.

199 ent is a general phenomenon occurring during enzymatic digestion of proteins.

200 cid residues in peptides are observed during enzymatic digestion of proteins.

201 gning ion signals from peptides arising from enzymatic digestion of proteins.

202 Enzymatic digestion of proteoglycans in the ECM restored

203 ented HGE cell binding and infection, as did enzymatic digestion of PSGL-1.

204 Enzymatic digestion of purified, interstrand cross-linke

205 idation instead of hydrolysis and to enhance enzymatic digestion of recalcitrant substrates including

206 a combination of MALDI-TOF and FAB MS and by enzymatic digestion of reduced derivatives, followed by

207 lity of these microfabricated sample plates, enzymatic digestion of ribonucleic acids was performed o

208 The procedure involves mechanical and enzymatic digestion of skeletal muscle tissue with colla

209 Enzymatic digestion of sulfated GAGs canceled their effe

210 by an increase in fluorescence signal due to enzymatic digestion of the antibody and separation of IR

211 erefore, a mild extraction protocol based on enzymatic digestion of the C. elegans cuticle with high-

212 mixture of oligoporphyrans derived from the enzymatic digestion of the cell wall of the red alga P.

213 Enzymatic digestion of the cell wall resulted in the rel

214 rate the dominant influence of lignin on the enzymatic digestion of the cell wall.

215 id chromatography is usually performed after enzymatic digestion of the cross-linked proteins.

216 Moreover, enzymatic digestion of the DNA plasmid (pBR322, 4361 bp)

217 examining a mixture of peptides generated by enzymatic digestion of the equine cytochrome c with tryp

218 ate phagolysosomes, which play a key role in enzymatic digestion of the internalized contents into co

219 We achieve this objective by combining enzymatic digestion of the meat, hydrodynamic cavitation

220 e composition analysis is then performed via enzymatic digestion of the oligonucleotide to complement

221 light mass spectrometry (MALDI-TOF MS) after enzymatic digestion of the polysaccharide component.

222 However, enzymatic digestion of the protein in MBSE removed its a

223 ploying multiple peptides to ensure that the enzymatic digestion of the protein is efficient in the r

224 able IsoLG adducts on apolipoprotein B after enzymatic digestion of the protein to individual amino a

225 idation of low density lipoprotein following enzymatic digestion of the protein to single amino acids

226 s identified using phage display and partial enzymatic digestion of the purified protein.

227 ynitrite-treated DNA was characterized after enzymatic digestion of the reacted DNA to the nucleoside

228 Enzymatic digestion of the retained RNA supported the co

229 Enzymatic digestion of the RNA oligomer catalyzed by bov

230 The procedure does not involve mechanical or enzymatic digestion of the sample tissue.

231 Enzymatic digestion of the surrounding matrix resulted i

232 Enzymatic digestion of the telomeric G-quadruplex struct

233 equenced after site-directed mutagenesis and enzymatic digestion of the wild-type allele.

234 e level (57 and 55%, respectively) following enzymatic digestion of their hyaluronic acid capsule.

235 Enzymatic digestion of these radiolabeled products produ

236 essor cells are due to the loss of CD44 upon enzymatic digestion of tumors.

237 The IDMS method involves enzymatic digestion of viral proteins and the specific d

238 Enzymatic digestions of membrane proteins are also great

239 Enzymatic digestions of proteins that are resistant to p

240 Following electrophoresis and renaturation, enzymatic digestions of the substrate in 50 mM of Tris b

241 method was developed incorporating cyanogen, enzymatic digestion, one-dimensional gel electrophoresis

242 rgeting 5-methyldeoxycytidine moieties after enzymatic digestion, only 5-meC specifically derived fro

243 ostnatal microfibrils and can be revealed by enzymatic digestion or by genetic ablation of Fbn1.

244 Mass spectrometry (MS) in conjunction with enzymatic digestion or chemical probes has been widely u

245 vivo in which the size of HA was defined by enzymatic digestion or custom synthesis.

246 It should be noted that enzymatic digestion or fermentation by microbiota could

247 The CATCH assay requires no enzymatic digestion or ligation, requires little startin

248 circumneutral pH conditions employed during enzymatic digestion prevent artifacts arising from extre

249 In addition, a combination of different enzymatic digestion procedures (i.e., glycosidase, siali

250 We present a rapid and efficient in-solution enzymatic digestion protocol suitable for mass spectrome

251 ical sample, conventional RNA extraction and enzymatic digestion protocols that are employed prior to

252 no-ESI MS method in combination with limited enzymatic digestion provides a sensitive method for dire

253 lage using the surface forces apparatus, and enzymatic digestion, reveal an "adaptive" role for an HA

254 deformed cellulose fibrils following partial enzymatic digestion reveals that processive cellulases e

255 hilic interaction chromatography followed by enzymatic digestion specifically at lysine or arginine r

256 Tissues were dissociated by enzymatic digestion, stained with panels of antibodies t

257 zing reagents, different storage conditions, enzymatic digestion, stator-rotor and bead motion-based

258 and protein quantification by LC-MS/MS, the enzymatic digestion step and the internal standardizatio

259 PCRs included an enzymatic digestion step to eliminate PCR carryover and

260 l bottom-up MS analysis workflows require an enzymatic digestion step which can be time consuming and

261 onfirm the 100% successful completion of the enzymatic digestion step.

262 ctose was further characterized via multiple enzymatic digestion steps including mannosidase with act

263 The use of a multiple and sequential enzymatic digestion strategy was important in producing

264 5E/Q180E mutant also was more susceptible to enzymatic digestion, suggesting increased solvent access

265 ate the efficacy of using acid, alkaline and enzymatic digestion techniques in mineralizing biologica

266 After simulated enzymatic digestion, the oxygen radical absorbance capac

267 s are applied to a tissue section, directing enzymatic digestion to a spatially confined area of the

268 ange) to the regional level by incorporating enzymatic digestion to acquire binding information for p

269 hods were established in this study based on enzymatic digestion to consolidate peaks of the same typ

270 Enzymatic digestion to form 3-(2-deoxy-beta-d-erythro-pe

271 5a) and reduction with NaCNBH(3) followed by enzymatic digestion to give 1,3-bis(2'-deoxyguanosin-N(2

272 describe a method that uses restricted Lys-C enzymatic digestion to increase the average mass of prot

273 adducted oligonucleotide 26 was provided by enzymatic digestion to nucleosides and authentic adduct

274 and spotted samples first undergo on-surface enzymatic digestion to release N-glycans.

275 ent the combined application of chemical and enzymatic digestions toward de novo sequence determinati

276 his approach, isotopic labeling involves the enzymatic digestion, typically performed with trypsin, o

277 human serum albumin (HSA), was subjected to enzymatic digestion using 12 different sample preparatio

278 Samples are subjected to selective enzymatic digestion using a bacterial hyaluronidase (HA

279 mechanical disruption during digestion, warm enzymatic digestion using enzyme collagenase:NP activity

280 Here, enzymatic digestion using microsomal alanyl aminopeptida

281 However, heat combined with enzymatic digestion was necessary to markedly lower IgE

282 Complete enzymatic digestion was slowed down by 3 hours in the cr

283 best performing, base hydrolysis followed by enzymatic digestion, was validated and used to analyse 2

284 ion (FASP), and microwave-assisted on-filter enzymatic digestion, we identified 2 090 proteins from t

285 evels of incurred tissue samples prepared by enzymatic digestion were in good agreement with the valu

286 deglycosylated peptides analysis, different enzymatic digestions were performed and glycopeptide det

287 The guarana samples before and after enzymatic digestion, were compared for total phenolic co

288 ation mass spectrometry, in combination with enzymatic digestions, were used to verify the amino acid

289 of antioxidant compounds was obtained after enzymatic digestion, whereas the highest proportion was

290 cellular compositions and without the use of enzymatic digestion, which causes undesirable molecular

291 re significantly, the utilization of limited enzymatic digestion with a cysteine protease as compared

292 and metals were simulated by using in vitro enzymatic digestion with a gastric fluid solution and di

293 In this work, we combine enzymatic digestion with cryogenic IR-spectroscopy and d

294 ified by polyacrylamide gel electrophoresis, enzymatic digestion with exonuclease VII and electrospra

295 xcised from the oxidized oligonucleotides by enzymatic digestion with nuclease P1 and alkaline phosph

296 ered the need when applying a combination of enzymatic digestion with nuclease P1 and tandem mass spe

297 e exclusion chromatography, before and after enzymatic digestion with pepsin or trypsin.

298 d by the release of fluorophores by means of enzymatic digestion with proteinase K.

299 Enzymatic digestion with proteinases liberated sCD44 fro

300 rigenesis, matrix components are modified by enzymatic digestion with subsequent effects on integrin