ライフサイエンスコーパス: enzyme assay

1 constitutively-active mutant (confirmed via enzyme assays).

2 l conversions of steroids as substrates (for enzyme assays).

3 inetic parameters were measured by a coupled enzyme assay.

4 where reagents were added for a fluorescent enzyme assay.

5 ese ligands was confirmed using a functional enzyme assay.

6 bility was assessed by using a mitochondrial enzyme assay.

7 enzyme activity over the course of a 20 min enzyme assay.

8 ation were characterized using a radioactive enzyme assay.

9 values found using the conventional coupled-enzyme assay.

10 1, showed an IC(50) of 140 pM in a cell-free enzyme assay.

11 ned for inhibitory activity in a traditional enzyme assay.

12 d using a novel, continuous protease-coupled enzyme assay.

13 was investigated using an in vivo complex of enzyme assay.

14 og, using a continuous, thermostable coupled enzyme assay.

15 bining 2D-gel electrophoresis and an on-blot enzyme assay.

16 ing IC(50)s < 400 nM in a partially purified enzyme assay.

17 This was confirmed experimentally by enzyme assay.

18 vo [18F] gamma counting and thymidine kinase enzyme assay.

19 e of HSV1-sr39TK protein by thymidine kinase enzyme assay.

20 creen were validated in a secondary in vitro enzyme assay.

21 ted cholesterol 27-hydroxylation by in vitro enzyme assay.

22 bserved between the two isomers in the GGDPS enzyme assay.

23 in recipients by in vivo imaging and direct enzyme assay.

24 nds of wholemeal bread, was also assessed by enzyme assay.

25 elomerase activity as measured by the direct enzyme assay.

26 lar analysis, EM, X-ray crystallography, and enzyme assays.

27 lished by in vitro testing using recombinant enzyme assays.

28 y desirable for enhancing the selectivity of enzyme assays.

29 essential in vivo but not revealed by simple enzyme assays.

30 ed by reverse phase HPLC for use in in vitro enzyme assays.

31 2 (MC2082), were tested in both cellular and enzyme assays.

32 ol labeling, mass spectrometry, and targeted enzyme assays.

33 affected, and confirm this hypothesis using enzyme assays.

34 ivity over the related Jak family kinases in enzyme assays.

35 of an Escherichia coli Tyr auxotroph and by enzyme assays.

36 ficities using novel mass spectrometry-based enzyme assays.

37 and must be generated as an integral part of enzyme assays.

38 und parallels its potency and selectivity in enzyme assays.

39 trated using a panel of purified recombinant enzyme assays.

40 oderate to high inhibition of IN in purified enzyme assays.

41 of NS5B by the triphosphates in the in vitro enzyme assays.

42 used substrate for glutathione S-transferase enzyme assays.

43 , Fe(2+), Ni(2+), and Zn(2+) in the in vitro enzyme assays.

44 mortem analysis using histologic staining or enzyme assays.

45 t proteins were monitored by SDS-PAGE and by enzyme assays.

46 , PcGSS4, and PcGSS5 showed weak activity in enzyme assays.

47 C-MS), nuclear magnetic resonance (NMR), and enzyme assays.

48 with shotgun metagenomics and extracellular enzyme assays.

49 confer resistance to FR171456 in growth and enzyme assays.

50 r screening and lacks reproducibility of the enzyme assays.

51 ically more complex than that for the single-enzyme assays.

52 In a cell-free enzyme assay, 21 showed a CDK2/cycE IC(50) = 48 nM and w

53 rein, a novel automatic and integrated micro-enzyme assay (AImuEA) platform was proposed based on a u

54 assay calibration did not interfere with the enzyme assay allowing both measurements to be performed

55 detected and quantified using a fluorogenic enzyme assay and a numerical model.

56 hibition of DNA gyrase as corroborated in an enzyme assay and by the inhibition of precursor thymidin

57 monstrate submicromolar activity both in the enzyme assay and in a (14)C-emulsion assay employing cho

58 Using an in vitro enzyme assay and LC-MS, methylofuran was identified in c

59 ound 1 displayed a TarO IC50 of 125 nM in an enzyme assay and possessed very high lipophilicity (clog

60 in (i) inhibited LF protease activity in an enzyme assay and protected macrophages against recombina

61 Using a fluorometric coupled enzyme assay and smooth muscle myosin light chain (MLC)

62 e is a useful tool for the alpha-glucosidase enzyme assay and will facilitate compound screening for

63 ave tested this hypothesis by using in vitro enzyme assays and a range of acyl-ACP, malonyl-ACP, and

64 L-Trp, the reactivation was demonstrated by enzyme assays and by various spectroscopic techniques.

65 Validation through enzyme assays and customized (13) C metabolite profiling

66 These results were observed in both in vitro enzyme assays and flow cytometric analyses of Chinese ha

67 Together with enzyme assays and HPLC analyses of pollen extracts from

68 Enzyme assays and IFN-beta reporter assays of cGAS mutan

69 Enzyme assays and immunoblot analyses indicated that 5-F

70 rase and sialyltransferase activity based on enzyme assays and microarray analysis.

71 ted by using the rapid (<5 min) amperometric enzyme assays and pH-sensitive iridium oxide films.

72 mbination of X-ray crystallography, in vitro enzyme assays and site-directed mutagenesis, of the baci

73 ombination of Sanger sequencing, restriction enzyme assays and targeted deep sequencing.

74 ein reductase FabI, as validated by in vitro enzyme assays and the generation of bacterial isolates w

75 In vitro enzyme assays and transmission electron microscopy showe

76 Enzyme assays and Western blots show that whole heart ho

77 ed through in vitro binding assays, in vitro enzyme assay, and through functional cellular assays.

78 ty of soluble form of CD73 using radioactive enzyme assays, and CD73 messenger RNA levels from leukoc

79 epancies, nuisance inhibition, sophisticated enzyme assays, and limited structural information about

80 stry, RNA interference, quantitative RT-PCR, enzyme assays, and lipidomic analyses of endocannabinoid

81 uding in silico molecular dynamics, in vitro enzyme assays, and measures of serotonin (5-HT) plasma c

82 st of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were es

83 uently confirmed with reporter gene fusions, enzyme assays, and real-time PCR.

84 ctivity against the antibiotic cefotaxime in enzyme assays, and the mutant enzymes all lost thermodyn

85 Enzyme assays are important for many applications includ

86 Because the current enzyme assays are nonisoform specific, we examined assoc

87 19 affected TOP2 enzyme activity in in vitro enzyme assays as well as in live cells, we conclude that

88 is is often rate-limiting in high-throughput enzyme assays, as manual inspection and selection of a l

89 freed glucose was then detected by a coupled-enzyme assay at either a nitrogen-doped carbon nanotube

90 his work, we introduce an entirely automated enzyme assay based on capillary electrophoresis coupled

91 Enzyme assay buffer and crude dog plasma caused signal s

92 ittle tolerance for substitution in purified enzyme assays, but a few analogues retained MIC, presuma

93 ces the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encap

94 ectively inhibit HDAC6 by a recombinant HDAC enzyme assay, by determining the protein acetylation lev

95 The enzyme assay can be conducted in aqueous solution where

96 ncluded that kinetically based, stopped-flow enzyme assays can be performed in 60 s or less with a mi

97 ors of cPLA 2 alpha in a variety of isolated enzyme assays, cell based assays, and rat and human whol

98 t from the perfusion chip was pumped into an enzyme assay chip for monitoring of secretion from the c

99 In addition, enzyme assays, chlorophyll content and light microscopy

100 First, a coupled enzyme assay clearly demonstrated the ability of thiamin

101 An enzyme assay compatible with high-throughput screening (

102 targeted, functional ferrochelatase based on enzyme assays, complementation, and inhibitor studies.

103 ) is localized to the flagella, and in vitro enzyme assays confirm that the protein is a MAP kinase.

104 A colorimetric enzyme assay confirmed these findings.

105 Metabolic labeling and in vitro enzyme assays confirmed direct inhibition of the cytosol

106 Enzyme assays confirmed that most of these compounds are

107 Enzyme assays confirmed that these residues are absolute

108 In vitro enzyme assays confirmed that VTE3 is the plant functiona

109 dopsis enzyme (AtFMN/FHy) increased when the enzyme assays contained 0.02% Tween 20.

110 In a coupled enzyme assay containing GTR and wild-type GSAT, addition

111 make the ICECEA competitive with the optical enzyme assays currently in use.

112 In vitro enzyme assays demonstrate that all seven BTPSL genes fro

113 hanges in mitochondrial proteins, and direct enzyme assay demonstrated changes in both complexes I an

114 E. coli, and the use of FACE as an in vitro enzyme assay detected possible substrate preferences for

115 demonstrate that the cell-based assay and GO enzyme assay developed in this study are useful for furt

116 he presence of other histone peptides in the enzyme assay, enabling investigation of cross-reactivity

117 Equally small values in an RdpA enzyme assay (epsilonea = -1.0 +/- 0.1 per thousand) and

118 ces of this interaction were studied through enzyme assay experiments, where DAO enzymatic activity w

119 For fluorometric enzyme assay (FA) 1 (FA-1), 2'-(4-methylumbelliferyl)-al

120 port the development of a continuous coupled enzyme assay for FDPS activity that involves the subsequ

121 ers, we were also able to establish a robust enzyme assay for measuring bsNOS activity and inhibition

122 ovel, thermostable adaptation of the coupled-enzyme assay for monitoring glucose concentrations was d

123 A coupled-enzyme assay for MshC was developed using pyrophosphatas

124 ed IC(50)s consistent with those measured by enzyme assay for the activated form of biotin-p38alpha.

125 es were synthesized and tested in a p38alpha enzyme assay for their inhibition of tumor necrosis fact

126 ta analysis have allowed us to develop basic enzyme assays for substrate specificity and inhibitor ac

127 Enzyme assays further confirmed the identities of both N

128 The on-line enzyme assay had a limit of detection (LOD) of 4 microM

129 acylation and signaling assays as no direct enzyme assay had yet been developed.

130 To date, no enzyme assay has been developed for the Ddi1 proteins, b

131 Enzyme assays have revealed that the farnesyl pyrophosph

132 inhibitor of anaplastic lymphoma kinase (ALK enzyme assay IC(50) = 0.174 muM) during high throughput

133 rnally calibrated electrochemical continuous enzyme assay (ICECEA) and electrode stability allowed us

134 rnally calibrated electrochemical continuous enzyme assay (ICECEA) has proved to work well for single

135 rnally calibrated electrochemical continuous enzyme assay (ICECEA).

136 rnally calibrated electrochemical continuous enzyme assay (ICECEA, patent pending) was developed for

137 Enzyme assays, immunoblotting, immunohistochemical testi

138 mical characteristic of lipid I renders MurX enzyme assays impractical for screening and lacks reprod

139 me-free preassay calibration with the actual enzyme assay in one continuous experiment.

140 Enzyme assays in presence of protease inhibitors indicat

141 of the work was to perform kinetically based enzyme assays in the stopped-flow mode using a system of

142 No luciferase activity was detected by enzyme assays in tissue homogenates of BM recipients, an

143 d number of candidates were characterized by enzyme assays in vitro, gene expression analysis, non-po

144 studies of interactions with ARF6, in vitro enzyme assays, in vivo toxicity assays, and in vivo proc

145 tus Bath, along with NADH and duroquinol, to enzyme assays increased the activity of purified prepara

146 Enzyme assays indeed confirmed an electron transferase a

147 The in vitro enzyme assays indicate that these distinct metabolic phe

148 es complementation experiments, and in vitro enzyme assays indicate that this P450 is a ferulic acid/

149 Enzyme assays indicated both CGL and CBS enzyme activity

150 ene sequence, reverse transcriptase PCR, and enzyme assays indicated that dntD dntE ORF13 dntG compos

151 In vitro enzyme assays indicated that its preferred substrate is

152 ogues were synthesized, and preliminary PP2A enzyme assay inhibition studies were performed for the f

153 We describe the development of an enzyme assay inside picoliter microdroplets.

154 ounds were tested in several serine protease enzyme assays involved in the coagulation cascade exhibi

155 epared and tested in several serine protease enzyme assays involved in the coagulation cascade.

156 Following extraction, enzyme assays involving recombinant farnesyl protein tra

157 e absence of an independent thymidine kinase-enzyme assay is discussed.

158 In this study, an enzyme assay is presented that determines the average ch

159 sition of the assay solution for the coupled-enzyme assays is typically more complex than that for th

160 valuation of these analogs in a human MetAP2 enzyme assay led to the identification of several inhibi

161 ided fractionation using signal transduction enzyme assays led to the isolation of the novel spiroket

162 ays, the behavior of a drug in a biochemical enzyme assay may not accurately reflect its performance

163 with a specific activity of 200 Ci/mmol for enzyme assays, metabolic studies, and tissue imaging.

164 A coupled-enzyme assay method based on the chemiluminescent detect

165 The method provides a simple and reliable enzyme assay method that enables the rapid diagnosis of

166 embly, we utilized synthetic peptide mimics, enzyme assays, molecular dynamics calculations, and nati

167 We have used an in vitro enzyme assay, monitoring hdm2-catalyzed Ub transfer from

168 Using enzyme assays, NMR, mutagenesis, and deletion of kdgF, w

169 Coupled enzyme assays of AMP and pyrophosphate release in the re

170 Enzyme assays of atrial tissue homogenates confirmed inc

171 One explanation that emerged from enzyme assays of deoxycytidine kinase (dCK) and deoxycyt

172 Map-based cloning of the KNF gene and enzyme assays of knf embryos demonstrated that KNF encod

173 The enzyme assays of mixed and coexpressed recombinant prote

174 Enzyme assays of MUR4 protein expressed in the methylotr

175 Enzyme assays of recombinant protein supported the hypot

176 were validated by Northern blot analysis and enzyme assays of selected genes.

177 Real-time PCR and PI-PLC enzyme assays of the TCS mutants, coupled with SrrA prom

178 Enzyme assays of wild-type embryos showed a rapid rise i

179 Enzyme assays on 17 isolates of borreliae demonstrated G

180 Enzyme assays on cell extracts localized total UDP-D-glu

181 on-modification (R-M) tests, direct in vitro enzyme assays or more recently from bacterial genome seq

182 Experimental data from continuous enzyme assays or protein folding experiments often conta

183 peptides for other purposes, including other enzyme assays or protein-binding ligands.

184 chemical studies like protein purifications, enzyme assays, organelle isolation or determination of m

185 alizarin red S stain for mineralization, and enzyme assay (p-nitrophenol phosphate cleavage) for alka

186 activity as 3-MPA interfered with the PEPCK enzyme assay, particularly at 0.5 and 1 mM.

187 n methods (-4 +/- 4%) indicated that the new enzyme assay performed accurately.

188 e secreted amylase activity as determined by enzyme assay, plate assay, or Western blot analysis.

189 Two-step pore-limit electrophoresis with enzyme assay (PLENZ) is conducted in a single, straight

190 We used plant transformation, in vitro enzyme assays, population genetics and quantitative gene

191 chemistry, immunoblotting, and a fluorogenic enzyme assay presenilin-1 (required for gamma-secretase

192 o imaging analysis (R2=0.98) and by in vitro enzyme assay (R2=0.94).

193 -stat has the potential for applications for enzyme assays, reagentless pH control, acidity/alkalinit

194 was detected by modifying the chip to allow enzyme assay reagents to be mixed with dialysate as samp

195 orogenic substrate for the alpha-glucosidase enzyme assay, resorufin alpha-d-glucopyranoside.

196 ve immunocytochemistry (ICC), and 5-[(3)H]FC enzyme assay, respectively.

197 ium were 0.096, 0.108, and 2.3 muM in the GO enzyme assay, respectively.

198 sured by surface plasmon resonance (SPR) and enzyme assays, respectively.

199 Heterologous expression and enzyme assay reveal that 3 of these alleles encode sulfa

200 imulation, flow cytometry and a cell surface enzyme assay reveal that the C-terminal catalytic domain

201 Enzyme assays revealed that gli1 lacks glycerol kinase a

202 Enzyme assays revealed that Orf6 has a higher specific a

203 cs, real time reverse transcription-PCR, and enzyme assays revealed that the expression levels of som

204 e presence of a hydrolase (GXSXG) motif, and enzyme assays revealed the presence of monoacylglycerol

205 d-type or LPB-Tag prostates as determined by enzyme assay, reverse transcription-PCR, and immunohisto

206 a combination of mass spectrometry, RNA-seq, enzyme assays, RNAi and phylogenomics in different non-m

207 These lines were then assessed by detailed enzyme assay, RT-qPCR and fruiting.

208 Molecular dynamics simulations and enzyme assays show the use of a His-Ser dyad to catalyze

209 mer interface, thereby destabilizing and, as enzyme assays show, inactivating the enzyme.

210 In vitro enzyme assays showed a significant increase in forward P

211 (2) In vitro enzyme assays showed potent inhibition of JAK3- and Syk-

212 Cell-based enzyme assays showed selective inhibition of JAK1/3-depe

213 Enzyme assays showed significant 11 beta-HSD2 activity (

214 Accordingly, enzyme assays showed that 3-nitrotyramine and its oxidat

215 Enzyme assays showed that ATP was the preferred substrat

216 Enzyme assays showed that cell extracts from a pduL muta

217 The enzyme assays showed that CNT nearly doubled the retenti

218 Enzyme assays showed that expression strains produced 87

219 Enzyme assays showed that one of the adenylyltransferase

220 Although early in vitro enzyme assays showed that recombinant BAR and PAT exhibi

221 -4.4-fold increase of radioactivity, whereas enzyme assay shows 22.1+/-6.1-fold increase of thymidine

222 In contrast to results with individual enzyme assays, specificity for thioredoxin f or m was no

223 ions of the LDLR class A repeats by in vitro enzyme assays suggested a major role of GalNAc-T11.

224 Enzyme assays supported the structure-based predictions

225 hosphate, together with in vivo and in vitro enzyme assays, supports a pyridoxal phosphate-dependent

226 This paper describes a microfabricated enzyme assay system including a micromixer that can be u

227 This enzyme assay takes advantage of the amplification charac

228 n, expensive instrument) are overcome by the enzyme assay that is presented here, which allows for hi

229 an in vitro, single-well, fluorescence-based enzyme assay that monitors the first step of the PAT rea

230 e Erf2.Erf4 PAT, we have developed a coupled enzyme assay that monitors the turnover of the palmitoyl

231 ribe the development of cGAMP-Luc, a coupled enzyme assay that relies on the degradation of cGAMP to

232 Both had muscle mitochondrial enzyme assays that showed a pronounced mitochondrial hyp

233 In the enzyme assay, the average polyP chain length is calculat

234 nst clinically relevant HIV-1 mutants and in enzyme assays, the simultaneous C5(methyl)/C6(methyl/eth

235 (S)-enantiomer was detected using a coupled enzyme assay; The rate of elimination of HF from the (R)

236 rved association, using a custom restriction-enzyme assay to analyze the DNA in 158 samples from 29 p

237 .2 chemically and subjected it to an on-blot enzyme assay to characterize the activity.

238 eous time-resolved fluorescence (HTRF)-based enzyme assay to measure the catalytic activity of N(6)-m

239 The ability of (31)P NMR and the enzyme assay to size polyP was demonstrated with polyP l

240 s, immunohistochemistry, H&E and biochemical enzyme assays to determine the role of Src kinase on mit

241 electron paramagnetic resonance, and coupled enzyme assays to investigate how single mutations at pos

242 These protocols require from 1 d (enzyme assays) to up to 3 months (autoradiography of [(3

243 In enzyme assays, Trx1 activated two selected targets follo

244 Common enzyme assays use optical methods that often require the

245 The enzyme assay used here might find wider application in s

246 on rate and the sensitivity of low-abundance enzyme assay using a micro/nanofluidic preconcentration

247 f a rapid, simple, and robust LC-MS/MS-based enzyme assay using dried blood spots (DBS) for the diagn

248 We also developed a GO enzyme assay using the hydrogen peroxide-Amplex red repo

249 bstrate preference as determined by in vitro enzyme assays using 9-/13-hydroperoxy linolenic and 9-/1

250 In addition, in vitro enzyme assays using both (32)P- and (14)C-radiolabeled s

251 shown for select diTPSs, single and coupled enzyme assays using microbial and plant expression syste

252 Data from in vitro enzyme assays using microsomes showed that preexposure o

253 g each of the nine cDNAs were active in LACS enzyme assays using oleic acid as a substrate.

254 Enzyme assays using the sugar donor beta-d-arabinofurano

255 d through optimization of successive coupled enzyme assays using UDP-n-acetylglucosamine as the initi

256 Cell-free enzyme assays, using the crude Ayg1p protein extract, re

257 We present a deuterium-label based enzyme assay, utilizing a series of peptide substrates w

258 Enzyme assays validated the increase in isocitrate lyase

259 d adipocytes with on-line fluorescence-based enzyme assay was developed to monitor glycerol secretion

260 A sensitive fluorescent enzyme assay was used to quantify expression levels for

261 intensity of the fluorescent product of the enzyme assay was used to quantify the glucose concentrat

262 A deuterium-label-based coupled-enzyme assay was used to rapidly determine the stereoche

263 A colorimetric enzyme assay was used to screen for oxalate oxidase acti

264 channels designed for rapid electrophoretic enzyme assays was developed.

265 Linearity of the enzyme assays was seen for both time (0-16 min) and cyto

266 Using an HPLC-based MalQ enzyme assay, we could demonstrate that the equilibrium

267 Using an in vitro enzyme assay, we determined that all four proteins are M

268 s of Pseudomonas aeruginosa, and an in vitro enzyme assay, we have identified both PMM and PGM activi

269 Using reconstituted enzyme assays, we also demonstrated that sertraline inhi

270 ochemistry, western blotting, and functional enzyme assays, we assessed the distribution, quantity, a

271 specificity conflict through direct in vitro enzyme assays, we conducted PGP gene knockout studies in

272 Through microscopy and enzyme assays, we demonstrated the particles were able t

273 se of domain deletions, binding studies, and enzyme assays, we find that the WD40 repeats confer a sa

274 Interestingly, using HMGL enzyme assays, we found that rather than HMGL46, homodim

275 recombinant protein expression and in vitro enzyme assays, we provide evidence for several additiona

276 Using in vitro enzyme assays, we show that the C-terminal tail portion

277 ral information with sequence alignments and enzyme assays, we were able to elucidate determinants of

278 approximately 10-fold improved potency in an enzyme assay were identified, and this improved activity

279 e ICECEA in the development of other coupled-enzyme assays were also discussed.

280 Enzyme assays were also performed with IP as an indicato

281 ass spectrometry (MS) data were supported by enzyme assays, Western blot, and histochemistry.

282 utilization or depletion of carbon sources, enzyme assays, Western blotting and mass spectrometric a

283 over 70% inhibition activity at 50 nM in the enzyme assay, whereas the corresponding C-4 regioisomers

284 method for a universal fluorescence turn-on enzyme assay, which relies on the target enzyme-triggere

285 Phytol feeding and enzyme assays with Arabidopsis and recombinant Escherich

286 concordance existed among Q-RT-PCR, ICC and enzyme assays with both cell lines.

287 In vitro enzyme assays with candidate genes from the region of th

288 hibiting fungal endo-polygalacturonases, but enzyme assays with extracts of AS-SHY pollen do not supp

289 Enzyme assays with NAA80 and different profilins demonst

290 In coupled enzyme assays with purified PFOR and FldA, FqrB reduced

291 Heterologous expression and in vitro enzyme assays with purified RPHs from diverse bacterial

292 e polyprenol-deficient yeast rer2 mutant and enzyme assays with recombinant AtCPT7 confirmed that the

293 In vitro enzyme assays with recombinant CrNCED1 protein showed th

294 ynthase to the epithelium of glands and used enzyme assays with recombinant protein of the same gene

295 omplementation tests in Escherichia coli and enzyme assays with recombinant proteins confirmed that A

296 and retina isomerases were then measured by enzyme assays with specific enzyme inhibitors.

297 th Escherichia coli mutants, by the in vitro enzyme assays with the recombinant proteins, and by the

298 Using metabolite analysis, enzyme assays with WRC tissue extracts, cloning, and fun

299 d shown to inhibit BoNT/A LC in a FRET-based enzyme assay, with confirmation in an HPLC-based assay.

300 Using enzyme assays, X-ray crystal structures, and simulations