ライフサイエンスコーパス: enzyme reaction

1 nsfer effects and reduced efficiencies of an enzyme reaction.

2 trol conformational changes required for the enzyme reaction.

3 luorescent product, resorufin, via a coupled-enzyme reaction.

4 lso defines key residues responsible for the enzyme reaction.

5 ve site that are thought to be important for enzyme reaction.

6 metabolized to malate followed by the malic enzyme reaction.

7 ty change cooperatively occurs in continuous enzyme reaction.

8 dynamics and the catalytic redox step of the enzyme reaction.

9 ding sites of the enzyme changes (lowers) in enzyme reaction.

10 tivity effects that has been reported for an enzyme reaction.

11 o generate the pyruvate product in the malic enzyme reaction.

12 the molecule could be asymmetric during the enzyme reaction.

13 the bioluminescence produced by the coupled enzyme reaction.

14 ng due to a kinetic bottleneck following the enzyme reaction.

15 ) represented the full isotope effect of the enzyme reaction.

16 g its entire length during all stages of the enzyme reaction.

17 alactopyranoside, as the model system of the enzyme reaction.

18 ylation side activity next to their original enzyme reaction.

19 p to determine the key kinetic parameters of enzyme reaction.

20 rometry confirms the expected product of the enzyme reaction.

21 Cofactors are essential for many enzyme reactions.

22 nterpreting 18O kinetic isotope effects upon enzyme reactions.

23 in comparisons of changes in patterns during enzyme reactions.

24 tial rates from nonlinear progress curves of enzyme reactions.

25 the transient-state time courses of the two enzyme reactions.

26 e prehydride charge-transfer complex in both enzyme reactions.

27 enzymes and can perform all seven classes of enzyme reactions.

28 inetics is an essential model to rationalize enzyme reactions.

29 tive material to boost efficiency of oxidase enzyme reactions.

30 g how light energy can be harnessed to power enzyme reactions.

31 n of the corresponding genes and by in vitro enzyme reactions.

32 te how this impacts on experimental KIEs for enzyme reactions.

33 on studies of interacting systems as well as enzyme reactions.

34 es covering ~1800 (70%) of sequence assigned enzyme reactions.

35 oach for studying the mechanistic details of enzyme reactions.

36 rganic enzyme cofactors are involved in many enzyme reactions.

37 MM molecular dynamics approach in simulating enzyme reactions.

38 e that the method is suitable for monitoring enzyme reactions also in complex biological cellular hom

39 PA levels, which are regulated by the enzyme reaction, also control the expression of UAS(INO-

40 s a nucleophilic catalyst in both the normal enzyme reaction and in the formation of a covalent compl

41 We review characterization of the enzyme reaction and in vivo studies in which Sprague-Daw

42 ling and the capacity of CD38 to catalyze an enzyme reaction and produce cADPR, ADPR, and/or nicotina

43 titative relationship between the rate of an enzyme reaction and the concentration of its substrate.(

44 iology, occurring, for example, in proteins, enzyme reactions and across proton channels and pumps.

45 incorporates all of the forward and reverse enzyme reactions and regulatory circuits of the branched

46 for the general mechanistic understanding of enzyme reactions and the role that flexible loops play i

47 In order to understand the evolution of enzyme reactions and to gain an overview of biological c

48 reference database, consists of metabolites, enzymes, reactions and metabolic pathways.

49 Cyc as a reference, consists of metabolites, enzymes, reactions and metabolic pathways.

50 ors and cofactors, on the initial rate of an enzyme reaction, and it could be applied to a comprehens

51 amic links for microbial metabolic pathways, enzyme reactions, and their substrates and products.

52 In this method, enzyme reactions are carried out in solution using subst

53 nd ET efficiency that may arise when complex enzyme reactions are driven by artificial light absorber

54 Our earlier studies show that the two enzyme reactions are linked by the coenzyme product, NAD

55 e of protein conformational changes in these enzyme reactions are summarized.

56 In vitro enzyme reactions are traditionally conducted under condi

57 has increasingly been found to contribute to enzyme reactions at room temperature.

58 from traditional approaches used to analyze enzyme reactions at steady state, but they are also appl

59 ter, prespotted reaction volumes to activate enzyme reactions at targeted positions on a microarray.

60 for quantitative similarity searches between enzyme reactions at three levels: bond change, reaction

61 or a given three-dimensional active site and enzyme reaction, based on a set of catalytic rules compi

62 The separated products of the enzyme reaction can be quantitated by radiometric scanni

63 equence of this flexibility is that the same enzyme reaction can occur via multiple reaction pathways

64 t with simpler molecular mechanical methods, enzyme reactions can be modeled.

65 The kinetic parameters of the enzyme reaction catalyzed by the ectoenzyme pantetheinas

66 pathways, elements with multiple functions, enzyme reaction chains, and equilibrium enzymes.

67 y 7-fold in the dehydration direction of the enzyme reaction compared to wild-type (WT).

68 After stopping the enzyme reaction, compounds were extracted by perchloric

69 iciency (E(d)), substrate concentration, and enzyme reaction conditions were evaluated.

70 4alpha-demethylase activity in reconstituted enzyme reactions confirmed UDO and UDD as potent and sel

71 zzled and Chordin in the gastrula embryo and enzyme reaction constants were all in the 10(-8) M range

72 that lock topoisomerase II at a point in the enzyme reaction cycle where the enzyme forms a closed cl

73 ms and 2) the chemical rate constants of the enzyme reaction cycle.

74 OP2-DNA covalent complexes formed during the enzyme reaction cycle.

75 ty relationships, to study chemical steps in enzyme reaction cycles.

76 ion of this nonlinear regression approach to enzyme reactions demonstrated satisfactory results.

77 deoxygenation process, based on GOx-glucose enzyme reaction, depletes dissolved oxygen.

78 In this case, the enzyme reaction displays a later transition state compar

79 -color fluorescence assay to monitor coupled enzyme reactions during Okazaki fragment maturation is d

80 this system, increasing overall encapsulated enzyme reaction efficiency, factor(s) required for the p

81 The rates of enzyme reactions fall within a relatively narrow range.

82 e biosensor was developed exploiting coupled enzyme reactions for quantifying L-lactate in oral fluid

83 pete with plasma levels of Br- and steer the enzyme reaction from a 2e- oxidation to a 1e- oxidation

84 e acting as a genuine cofactor in the single-enzyme reaction, functions in the luciferase-coupled rea

85 ese studies underscore a concerted series of enzyme reactions governing histone modifications that pr

86 The complex catalytic mechanism of this enzyme reaction has emerged as the controversial subject

87 ments that lack this nutrient; thus, unusual enzyme reactions have also evolved to cleave the C-P bon

88 operties of rat FDH suggest that the overall enzyme reaction, i.e. NADP(+)-dependent conversion of 10

89 Studies of the molecular basis of the enzyme reaction in Triton micelles revealed that (a) a n

90 duct of many radical S-adenosyl-l-methionine enzyme reactions in all domains of life.

91 By initiating enzyme reactions in crystals, we have trapped hExo1 reac

92 al, we screen approximately 10(8) individual enzyme reactions in only 10 h, using < 150 microL of tot

93 standpoints, requires measuring the rates of enzyme reactions in their native environment and interpr

94 Negative cooperativity in enzyme reactions, in which the first event makes subsequ

95 al 5'-phosphate (PLP) is involved in diverse enzyme reactions including amino acid and hormone metabo

96 ansitions over the entire time-course of the enzyme reaction initiated by fast mixing of the enzyme a

97 Formation of the acetyl-S-enzyme reaction intermediate by Y130L, F204L, and Y376L

98 impaired formation of the covalent acetyl-S-enzyme reaction intermediate exhibited diminished (D159A

99 Formation of the [(13)C]acetyl-S-enzyme reaction intermediate of HMG-CoA synthase in D(2)

100 ethyl protons of HMG-CoA synthase's acetyl-S-enzyme reaction intermediate suggests a hydrophobic acti

101 ned structures a citrate anion forms an acyl-enzyme reaction intermediate with the catalytic threonin

102 , which indicate that E95A forms an acetyl-S-enzyme reaction intermediate with the same distinctive s

103 rmation of the covalent [1,2 -(13)C]acetyl-S-enzyme reaction intermediate.

104 y to form significant levels of the acetyl-S-enzyme reaction intermediate.

105 designed geometric factors of the substrate-enzyme reaction intermediates, such that catalysis is li

106 have proven to be very useful in identifying enzyme reaction intermediates.

107 In contrast to all other enzyme reactions investigated previously, including DHFR

108 Single-enzyme reactions involving abortive complexes, and rando

109 Its pathway operates through a cascade of enzyme reactions involving ubiquitin-activating (E1), ub

110 known mechanisms of the alkaline phosphatase enzyme reaction is tested to predict the measurements fo

111 An autocatalytic enzyme reaction is used to stimulate refolding of protei

112 y to monitor the progress of single-molecule enzyme reactions is often limited by the need to use flu

113 P69 dimerization is because of a crisscross enzyme reaction joining two substrate molecules bound to

114 We propose the slowing of enzyme reaction kinetics in the smaller droplets was the

115 nderscore the significant differences in the enzyme reaction kinetics with different substrate partic

116 uchel et al. for the analysis of consecutive enzyme reactions leads to a simple description of the ca

117 hy (PET) has been used clinically to measure enzyme reactions, ligand-receptor interactions, cellular

118 are achieved by synergistic or antagonistic enzyme reactions located within the helical connector or

119 nhibition by binding to the substrate of the enzyme reaction, making them examples of an unusual clas

120 rized enzymes; however, its Mg(2+)-dependent enzyme reaction mechanism may be analogous to one propos

121 r formation has no significant effect on the enzyme reaction mechanism.

122 alf-of-the-sites or flip-flop models for the enzyme reaction mechanism.

123 ng in this, the first committed, step of the enzyme reaction mechanism.

124 CiE database contains 223 distinct step-wise enzyme reaction mechanisms and holds representatives fro

125 out of proteins is important, both for many enzyme reaction mechanisms and proton pumping across mem

126 ces using the MACiE database of 202 distinct enzyme reaction mechanisms as a knowledge base.

127 Over the years, hundreds of enzyme reaction mechanisms have been studied using exper

128 elease of a web-based search tool to explore enzyme reaction mechanisms in MACiE.

129 Classification in Enzymes) is a database of enzyme reaction mechanisms, and is publicly available as

130 language extension that describes a suite of enzyme reaction mechanisms.

131 enzymes to the prediction and validation of enzyme reaction mechanisms.

132 work of one organism, including metabolites, enzymes, reactions, metabolic pathways, predicted operon

133 work of one organism, including metabolites, enzymes, reactions, metabolic pathways, predicted operon

134 the fluorescent labeling is performed on the enzyme reaction mixture.

135 ial electrophoretic assays from 16 different enzyme reaction mixtures at 20 s intervals in parallel.

136 Enzyme reaction mixtures cyclize (3RS)-[4,4,13,13,13-2H5

137 by assaying the amount of product formed in enzyme reaction mixtures that contained test compounds.

138 complexes are shown to serve as hydrogenase enzyme reaction models, H(2) uptake and H(2) production,

139 model to simulate the acetyl-CoA synthetase enzyme reaction network using the data derived from time

140 encoding various specific microscopic intra-enzyme reaction networks (micro-models), and (ii) lead t

141 cs models, will improve our understanding of enzyme reaction networks under nonideal conditions.

142 l chemical mechanism (catalytic steps) of an enzyme reaction, not only the overall reaction.

143 the endoplasmic reticulum membrane where its enzyme reaction occurs.

144 demonstrate the direction-dependent surface enzyme reaction of ExoIII with double-stranded DNA as we

145 We have demonstrated a multistep enzyme reaction on a chip to determine the key kinetic p

146 SEED) has been developed to measure multiple enzyme reactions on a monolith electrode due to immunosp

147 -HSD/isomerase) catalyzes the two sequential enzyme reactions on a single protein that converts dehyd

148 pearance of the final product of the coupled enzyme reaction or a decrease in the susceptibility of t

149 ty is achieved without the need for indirect enzyme reactions or specialized instrumentation.

150 iewpoint may be presumed to be applicable to enzyme reactions other than those of the alpha-amino aci

151 re a source of coenzymes for a vast array of enzyme reactions, particularly those of metabolism.

152 tate NMR has led to the postulation of a new enzyme reaction pathway and raised once again the questi

153 , provided the following key features of the enzyme reaction pathway.

154 Among them, previous studies have reported enzyme reaction pathways for octanal, 1-nonanal, vanilli

155 nted programming concepts to define glycans, enzymes, reactions, pathways and compartments for modeli

156 repair on thermal stability was confirmed by enzyme reactions performed over 0-45 degreesC.

157 on zone increased linearly with time over an enzyme reaction period of 30 min and at a rate that was

158 The coupled-enzyme reaction permits rapid signal amplification from

159 y for their applications in food processing, enzyme reactions, pharmaceuticals and cosmetics.

160 Both products of the CD38 enzyme reaction play important roles in signal transduct

161 To determine the rate-limiting step in the enzyme reaction, pre-steady-state kinetic analyses were

162 The enzyme reaction precursors are formed easily from two re

163 The transient kinetic behavior of enzyme reactions prior to the establishment of steady st

164 The enzyme reaction product of this new substrate emits at a

165 The electrochemical oxidation of the enzyme reaction product, 1-naphtol, measured by differen

166 e the rate of molecular self-assembly of the enzyme reaction product.

167 The cell-free enzyme reaction products obtained were characterized by

168 First, the published enzyme reaction products of cyanuric acid hydrolase are

169 The radiolabeled enzyme reaction products were predominantly (95%) single

170 e studied by video microscopy of the stained enzyme reaction products.

171 cement by parahydrogen polarization, such as enzyme reactions, protein-protein interactions, and prot

172 CP mutants highlight different states of the enzyme reaction, providing an underlying structural basi

173 Enzyme reaction rate constant (k) was placed in the 0-4

174 he scheme makes it possible to calculate the enzyme reaction rate explicitly by combining chemical ki

175 probability is very small, the effect on the enzyme reaction rate is considerably larger, for example

176 that Trp-11 is involved in regulation of the enzyme reaction rate, contradictory to a previous sugges

177 MFA) has been widely used to measure in vivo enzyme reaction rates (i.e., metabolic flux) in microorg

178 e a theory for the temperature dependence of enzyme reaction rates which takes the form of an exponen

179 n enzymes are very rare, and the majority of enzyme reactions rely upon nucleophilic and general acid

180 gy coupling between ATP hydrolysis and other enzyme reactions requires the phosphorylation of substra

181 At the completion of the enzyme reaction, residual substrate is detected with an

182 The product of enzyme reaction, resorufin, exhibits fluorescence emissi

183 The product of enzyme reaction, resorufin, exhibits fluorescence emissi

184 zyme-linked immunosorbent assay, and coupled-enzyme reaction, respectively.

185 AB with the involvement of the 27 most-known enzyme reaction rules of 22 enzymes, as an extension of

186 ble biochemical reaction from a given set of enzyme reaction rules that allows the de novo synthesis

187 le biochemical pathways using a given set of enzyme reaction rules.

188 d amino acid residue functions that occur in enzyme reaction sequences using the MACiE database of 20

189 n signals require direct conduction from the enzyme reaction site to the signal analyzing unit.

190 lfatide from the total sulfatide used in the enzyme reaction (sulfatide-Azure A present in a parallel

191 cs in the form of a highly coordinated multi-enzyme reaction system.

192 Our results suggest that pairs of similar enzyme reactions tend to proceed by different mechanisms

193 is approximately three times greater for the enzyme reaction than the uncatalyzed reaction, and the o

194 Here we show an enzyme reaction that operates effectively on a PNA/DNA h

195 The enzyme reactions that create these molecules are an inte

196 ytical methods are discussed and examples of enzyme reactions that have been successful on these surf

197 In the early days, radical enzyme reactions that use S-adenosylmethionine (SAM) coo

198 For this enzyme reaction, the large magnitude of the KIE arises m

199 We find that in a bimolecular enzyme reaction, the random mechanism is optimal over an

200 contribute, but, in contrast to unimolecular enzyme reactions, their role appears to be secondary to

201 tific microwave reactors can accelerate this enzyme reaction, they may not be easily accessible.

202 As a model enzyme reaction, this work examined the transfer of a me

203 Plots of ECL increase vs enzyme reaction time monitor relative rates of DNA damag

204 R, the initial slope of ECL increase versus enzyme reaction time normalized for amounts of enzyme an

205 The rate of increase in ECL with enzyme reaction time reflects relative DNA damage rates.

206 wave voltammetric (SWV) peaks increased with enzyme reaction time, and relative DNA damage rates at p

207 ions by using the output of an autocatalytic enzyme reaction to drive both the polymerization and sub

208 the initial velocities or the time course of enzyme reactions to an arbitrary molecular mechanism rep

209 bioreactors, allowing a range of significant enzyme reactions to be processed simultaneously.

210 ssel has been used in this work to carry out enzyme reactions under varying substrate concentrations.

211 F metabolites was measured subsequent to the enzyme reaction using catalytic voltammetric oxidation w

212 method requires nothing more than running an enzyme reaction using forcing concentrations of reactant

213 Enzyme reactions using a combination of human ER mannosi

214 incorporates direct hybridization and single enzyme reaction via the formation of single probe-RNA-pr

215 proach for a field-effect based detection of enzyme reactions via detecting changes in the pH value d

216 A more complex two-step enzyme reaction was also designed.

217 This enzyme reaction was measured continuously by positioning

218 ing, a state-of-the-art approach to simulate enzyme reactions, we have provided further evidence agai

219 AS, and Q147K ADCS mutants were prepared and enzyme reactions were analyzed by high-performance liqui

220 Products from enzyme reactions were identified by gas chromatography-m

221 A total of 335 enzyme reactions were retrieved from MACiE and were mapp

222 al tunneling and coupled motion in the malic enzyme reaction when NAD+ and malate are used as substra

223 ticularly useful in producing substrates for enzyme reactions when the dihydropterin substrate cannot

224 rapi oxide (de = 20-25%) are produced in the enzyme reaction, whereas two diastereomers of these comp

225 elease is the overall rate-limiting step for enzyme reaction with NGD+.

226 change of Escherichia coli ATCase during the enzyme reaction with physiological substrates.

227 rmation by SpyTag and SpyCatcher and suicide enzyme reaction with small-molecule ligands (HaloTag and

228 The assay integrates the enzyme reaction with the separation and is operated usin

229 pulation methods, we investigate restriction enzyme reactions with double-stranded (ds)DNA oligomers

230 ial crystallography studies, particularly of enzyme reactions with small molecule substrates.

231 to cold, we paired kinetic constants of 2223 enzyme reactions with their organism's optimal growth te

232 bstantial improvements by serving individual enzymes/reactions with accommodating conditions, we reas

233 ymes and a substrate for multiple regulatory enzyme reactions within and outside the cell.

234 ticle and offers a means of detecting single-enzyme reactions without fluorescence detection.