ライフサイエンスコーパス: error-prone PCR

1 brary of amide synthetase mutants created by error-prone PCR.

2 Second, a mutant library is generated by error-prone PCR.

3 sons were made with a library constructed by error-prone PCR.

4 ing sites and introduced random mutations by error-prone PCR.

5 riosus (Pfu-Pol), with superb performance in error-prone PCR.

6 major segment of which had been amplified by error-prone PCR.

7 nal mutagenesis methods like doped SELEX and error-prone PCR.

8 Furthermore, the error-prone PCR alone introduced the mutation with high

9 l. apply a screening approach that leverages error-prone PCR and a proliferating cell model to identi

10 the human coding region were synthesized by error-prone PCR and cloned directly in yeast by in vivo

11 wo methods of DNA manipulation are analysed: error-prone PCR and DNA recombination.

12 Error-prone PCR and DNA shuffling of the EK(L) gene, T7

13 , to generate such mutants, we have utilized error-prone PCR and fluorescence lifetime imaging to scr

14 ient method of mutagenesis that makes use of error-prone PCR and homologous recombination to generate

15 Five rounds of error-prone PCR and iterative screening were performed w

16 Using directed evolution techniques of error-prone PCR and mutant library screening, a variant

17 a high-throughput screening method utilizing error-prone PCR and next-generation sequencing to compre

18 of a novel technique that incorporates both error-prone PCR and recombineering.

19 Three rounds of error-prone PCR and selection identified a treble mutant

20 A single round of error-prone PCR and selection yielded variant ALR(Y274F)

21 Error-prone PCR and site-directed mutagenesis led to the

22 enopus ribosomal protein L5 was generated by error-prone PCR and used to delineate the binding domain

23 DNA sequence variations were generated with error-prone PCR and were inserted in the promoter region

24 the highest-affinity aptamers evolved using error-prone PCR, and 27- or 46-fold higher affinities th

25 improvements in expression, effected through error-prone PCR-based mutation within the signal peptide

26 so identified after mutagenesis of FXYD2b by error-prone PCR coupled with a selection for cell prolif

27 yield higher-performing variants faster than error-prone PCR-derived libraries.

28 brary containing scFv mutants was created by error-prone PCR, displayed on the surface of yeast, and

29 Large libraries of hGSTT1-1 constructed by error-prone PCR, DNA shuffling, or saturation mutagenesi

30 e reconstructions and to an experiment using error-prone PCR (EP-PCR) for large-scale sequence divers

31 enesis library, including those produced via error-prone PCR (ep-PCR), mutator Escherichia coli strai

32 Escherichia coli lsr operon promoters using error-prone PCR (ePCR) and selected for promoters that p

33 Error-prone PCR in realistic reaction conditions is pred

34 When mutations are enhanced through error-prone PCR, in vitro M2-seq experimentally resolves

35 Perhaps, the most popular method is the error-prone PCR, in which mistakes are introduced into a

36 Error-prone PCR is a DNA replication process that intent

37 method is further corroborated by assembling error-prone PCR libraries and regenerating templates fol

38 nalyzing four different replicative systems, error-prone PCR, mouse antibodies, a nematode, and Droso

39 variants identified during a single round of error-prone PCR mutagenesis and screening.

40 er improvements were facilitated by targeted error-prone PCR mutagenesis of loop-7, and additional PT

41 Error-prone PCR mutagenesis reinforced the importance of

42 such mutants by a new approach that utilizes error-prone PCR mutagenesis, overlap extension PCR, and

43 Our findings demonstrate how optimal error-prone PCR mutation rates may be calculated, and in

44 od for random mutagenesis of cloned genes by error-prone PCR or DNA shuffling that eliminates the nee

45 ends observed among libraries constructed by error-prone PCR, preservation of function was observed t

46 Here, we show experimentally that error-prone PCR produces a broader non-Poisson distribut

47 First, the AAV2 cap gene is amplified in an error-prone PCR reaction and further diversified through

48 A collection of point mutations generated by error-prone PCR revealed two small regions required for

49 uorescence-based screen, in conjunction with error-prone PCR/saturation mutagenesis, for altering the

50 h this inhibitor, we randomly mutagenized by error-prone PCR the E. coli dsbB gene and selected dsbB

51 ed on structure-function considerations, and error-prone PCR to create random mutations.

52 We compared the results of using NRR and error-prone PCR to evolve DNA aptamers that bind strepta

53 tution at amino acid 42 was discovered using error-prone PCR to generate additional mutations.

54 Here, we used error-prone PCR to mutagenize the full-length human TS c

55 The mutant Pfu-Pols can be applied in error-prone PCR, under exactly the same conditions used

56 Error prone PCR was used to construct a mutagenized PAI-

57 Error-prone PCR was combined with in vivo functional sel

58 f mutation frequency on affinity maturation, error-prone PCR was used to generate libraries containin

59 1) efficient in vitro cDNA mutagenesis (here error-prone PCR was used), 2) efficient retroviral deliv

60 By using error-prone PCR, we have isolated and characterized thre

61 olution, using active-site randomization and error-prone PCR, yielded a MetRS variant (designated Pra