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1 3 degrees C) (i.e. 10-20 times faster than N-ethylmaleimide).
2 teines by oxidation and reaction with NEM (N-ethylmaleimide).
3 blocking the S-nitrosylation reaction with N-ethylmaleimide.
4 to be predominantly modified by thiols or N-ethylmaleimide.
5 e-sensitive factor attachment protein, and N-ethylmaleimide.
6 -nitrosoglutathione, hydrogen peroxide, or N-ethylmaleimide.
7 atment of cells with low concentrations of N-ethylmaleimide (10 microM), suggesting that enrichment w
13 residues to the membrane-permeant reagent N-ethylmaleimide and the membrane-impermeant reagent polye
15 bited by sodium vanadate, sodium fluoride, N-ethylmaleimide, and phenylglyoxal but was not significan
16 species are trapped by cycloaddition with N-ethylmaleimide, and the reactions are traced by high res
17 ve been shown to be covalently modified by N-ethylmaleimide, and this treatment was found to block th
18 ns for activity, was potently inhibited by N-ethylmaleimide, and was labile at temperatures above 40
20 calcium-dependent Syt1 binding to soluble N-ethylmaleimide attachment protein receptor (SNARE) and v
21 on of Gbetagamma subunits with the soluble N-ethylmaleimide attachment protein receptor (SNARE) compl
22 Although FAK inhibition decreased soluble N-ethylmaleimide attachment protein receptor (SNARE)-media
23 a Golgi-localized target membrane-soluble N-ethylmaleimide attachment protein receptor (t-SNARE) pro
24 nt modification of its free cysteines with N-ethylmaleimide blocked binding to UL11 but not UL21.
25 the membrane-permeable sulfhydryl blocker, N-ethylmaleimide, by the RGD peptide, and by anti-alphaIIb
28 ed the role of a vesicle-residing, soluble N-ethylmaleimide factor attachment protein receptor (v-SNA
29 semolina protein (g protein) or 13.8 mumol N-ethylmaleimide/g protein reduces gliadin-glutenin cross-
31 f the completely conserved Cys353) through N-ethylmaleimide modification or mutagenesis to alanine ab
32 e redox states of both the as purified and N-ethylmaleimide-modified forms, using the combination of
33 ent of muscle cells with the NSF inhibitor N-ethylmaleimide, mutation of NSF, or suppression of NSF e
37 e have investigated the mechanism by which N-ethylmaleimide (NEM) enhances transporter activity using
38 erythrocytes, the cysteine-modifying agent N-ethylmaleimide (NEM) has been shown to inhibit system y(
39 of the protein thiol groups on the MPI by N-ethylmaleimide (NEM) markedly reduced this rate constant
40 ns isolated in the presence and absence of N-ethylmaleimide (NEM), a chemical that reacts irreversibl
41 rate and effect of Cys-159 modification by N-ethylmaleimide (NEM), a cysteine-selective alkylating ag
42 ridine, a known Isomerase I inhibitor, and N-ethylmaleimide (NEM), a known LRAT inhibitor, significan
43 d unfolding (CIU), chemical labeling using N-ethylmaleimide (NEM), and both bottom-up and top-down pr
45 lytes ((310)GSH and (616)GSSG), along with N-ethylmaleimide (NEM), and treated with acetonitrile to s
46 s cGMP independent but could be blocked by N-ethylmaleimide (NEM), indicating that NO acted via an S-
50 re more modest with a slight inhibition in N-ethylmaleimide (NEM, 1 mm)-treated RBCs and stimulation
51 current generated by the alkylating agent, N-ethylmaleimide, occluded the effect of H(2)O(2) The H(2)
52 y (2-aminoethyl)-methane thiosulfonate and N-ethylmaleimide of cysteine mutant proteins were measured
53 hemical labeling by isotope-coded forms of N-ethylmaleimide or succinic anhydride to site-specificall
54 es (c = 8 mmol L(-1)), photoligations with N-ethylmaleimide (possible for lambda </= 390 nm) are idea
55 bove their optimums and by Ca(2+), Zn(2+), N-ethylmaleimide, propranolol, and the sphingoid bases sph
56 ganine) lipids, nucleotides (ATP and CTP), N-ethylmaleimide, propranolol, phenylglyoxal, and divalent
59 ally inhibits NSF/Sec18 activity than does N-ethylmaleimide, requiring the administration of only low
62 release machinery, this assay incorporates N-ethylmaleimide sensitive factor (NSF) and alpha-SNAP, wh
63 ecule binds our two model His(6) proteins, N-ethylmaleimide sensitive factor (NSF) and O(6)-alklyguan
64 reover, we provided evidence for a role of N-ethylmaleimide sensitive factor (NSF) in regulating MuSK
65 related to the single N domains in p97 and N-ethylmaleimide sensitive factor (NSF); N1 of Pex1 is mob
67 ion, enabling cooperation with the soluble N-ethylmaleimide sensitive factor adaptor protein receptor
68 significantly reduced formation of soluble n-ethylmaleimide sensitive factor adaptor protein receptor
69 Evolutionarily conserved SNARE (soluble N-ethylmaleimide sensitive factor attachment protein recep
71 nct combinations of Munc18 and the soluble N-ethylmaleimide sensitive factor attachment protein recep
74 s study identifies the function of soluble N-ethylmaleimide sensitive factor attachment protein recep
75 a role for tethering complexes in soluble N-ethylmaleimide sensitive factor attachment protein recep
76 main, which likely participates in soluble N-ethylmaleimide sensitive factor attachment protein recep
77 tudy pinpoints the pivotal role of soluble N-ethylmaleimide sensitive factor attachment protein recep
78 in, complexin, and neuronal SNARE (soluble N-ethylmaleimide sensitive factor attachment protein recep
80 ining lipid bilayers as well as to soluble N-ethylmaleimide sensitive factor receptors (SNAREs) and p
85 the packaging of a SNARE protein (soluble N-ethylmaleimide-sensitive attachment protein receptor) oc
87 on secretion without altering its soluble N-ethylmaleimide-sensitive attachment receptor pairing wit
88 -1 (stabilizes assembled SNARE complexes), N-ethylmaleimide-sensitive factor (NSF) (disassembles SNAR
90 AAA domain-containing protein 1), soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein
91 uires membrane fusion mediated by soluble N -ethylmaleimide-sensitive factor (NSF) attachment protein
93 is directly involved in regulating soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein
94 main and modulate the affinity for soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein
95 oforms to directly regulate SNARE (soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein
100 ptor molecule for the SNARE-priming enzyme N-ethylmaleimide-sensitive factor (NSF) is known to be cru
101 ovel synaptic interaction between Arr1 and N-ethylmaleimide-sensitive factor (NSF) that is enhanced i
102 nhibits exocytosis by chemically modifying N-ethylmaleimide-sensitive factor (NSF), a key component o
103 kinase Cepsilon (PKCepsilon) regulates the N-ethylmaleimide-sensitive factor (NSF), an ATPase critica
104 hepatic secretion of VLDL-TAG by targeting N-ethylmaleimide-sensitive factor (NSF), both in vivo and
106 te a core complex of proteins comprised of N-ethylmaleimide-sensitive factor (NSF), soluble NSF attac
109 iverse cellular activities (AAA+) protein, N-ethylmaleimide-sensitive factor (NSF/Sec18), and its co-
110 ts, whereas further incubation with p97 or N-ethylmaleimide-sensitive factor (two AAA ATPases involve
111 presence of LPC as opposed to cholesterol, N-ethylmaleimide-sensitive factor + adenosine triphosphate
112 vely to the predicted syntaxin and soluble N-ethylmaleimide-sensitive factor accessory protein recept
113 r to prevent the completion of the soluble N-ethylmaleimide-sensitive factor activating protein recep
114 r C-terminus domain and the SNARE (soluble N-ethylmaleimide-sensitive factor activating protein recep
116 of the Rab GTPase Sec4 to promote soluble N-ethylmaleimide-sensitive factor adaptor protein receptor
117 of the Sec4 Rab GTPase to promote soluble N-ethylmaleimide-sensitive factor adaptor protein receptor
118 ined whether genetic disruption of soluble N-ethylmaleimide-sensitive factor attached protein (SNARE)
120 The assembly of the three neuronal soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP
122 ARE) complexes in conjunction with soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP
123 gnate acceptor compartments before soluble N-ethylmaleimide-sensitive factor attachment protein (SNAR
124 (SNAP-25) is a key molecule in the soluble N-ethylmaleimide-sensitive factor attachment protein (SNAR
125 s study, we identify the vesicular soluble N-ethylmaleimide-sensitive factor attachment protein recep
126 ar machines that are essential for soluble N-ethylmaleimide-sensitive factor attachment protein recep
127 llular transport vesicles requires soluble N-ethylmaleimide-sensitive factor attachment protein recep
128 abnormal expression or function of soluble N-ethylmaleimide-sensitive factor attachment protein recep
130 tro by arresting the late steps of soluble N-ethylmaleimide-sensitive factor attachment protein recep
131 an open conformation to accelerate soluble N-ethylmaleimide-sensitive factor attachment protein recep
132 ansmitters and hormones depends on soluble N-ethylmaleimide-sensitive factor attachment protein recep
133 from beta-cells, specifically the soluble N-ethylmaleimide-sensitive factor attachment protein recep
134 ice expressing a dominant-negative soluble N-ethylmaleimide-sensitive factor attachment protein recep
135 Neuronal exocytosis is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein recep
139 t work suggested that ER-localized soluble N-ethylmaleimide-sensitive factor attachment protein recep
140 depends on efficient formation of soluble N-ethylmaleimide-sensitive factor attachment protein recep
143 ved increase in K(+) currents is a soluble N-ethylmaleimide-sensitive factor attachment protein recep
144 We identified a member of the soluble N-ethylmaleimide-sensitive factor attachment protein recep
145 ther at the plasma membrane before soluble N-ethylmaleimide-sensitive factor attachment protein recep
146 tic vesicles, including the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
147 aliana, include complexes, such as soluble N-ethylmaleimide-sensitive factor attachment protein recep
148 tate the formation of trans-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
149 ric levels with its cognate target-soluble N-ethylmaleimide-sensitive factor attachment protein recep
150 in membrane fusion events are the soluble N-ethylmaleimide-sensitive factor attachment protein recep
151 Membrane fusion is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein recep
152 depends on the disassembly of cis-soluble N-ethylmaleimide-sensitive factor attachment protein recep
153 r release and vesicle recycling in soluble N-ethylmaleimide-sensitive factor attachment protein recep
154 4 vesicle fusion reaction requires soluble N-ethylmaleimide-sensitive factor attachment protein recep
155 ganelles involves the formation of soluble N-ethylmaleimide-sensitive factor attachment protein recep
156 ctural relationships among SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein recep
157 Munc18c binds to the trans-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
158 fusogens from vesicle trafficking (soluble N-ethylmaleimide-sensitive factor attachment protein recep
159 s requires the concerted action of soluble N-ethylmaleimide-sensitive factor attachment protein recep
160 istence of the unproductive target soluble N-ethylmaleimide-sensitive factor attachment protein recep
161 oteins are important components of soluble N-ethylmaleimide-sensitive factor attachment protein recep
163 nsulin granules, is carried out by soluble N-ethylmaleimide-sensitive factor attachment protein recep
166 Platelet exocytosis is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein recep
167 the fusion pore induced by SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
169 e is comparable fusion of 4-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
170 general membrane fusion machinery-soluble N-ethylmaleimide-sensitive factor attachment protein recep
171 exocytosis by interacting with the soluble N-ethylmaleimide-sensitive factor attachment protein recep
172 synaptic proteins from the soluble SNARE (N-ethylmaleimide-sensitive factor attachment protein recep
173 .1 is postulated to be involved in soluble N-ethylmaleimide-sensitive factor attachment protein recep
174 le marker proteins, glutamate, the soluble N-ethylmaleimide-sensitive factor attachment protein recep
176 abeled vesicle-associated v-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
178 that syt1 might facilitate SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
180 ediated by the formation of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
181 c syntaxin 1A, and it can activate soluble N-ethylmaleimide-sensitive factor attachment protein recep
182 etween subsets of so-called SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
183 Arabidopsis (Arabidopsis thaliana) soluble N-ethylmaleimide-sensitive factor attachment protein recep
184 epeated release requires cycles of soluble N-ethylmaleimide-sensitive factor attachment protein recep
185 verexpression of ER/Golgi arginine soluble N-ethylmaleimide-sensitive factor attachment protein recep
186 components: vacuolar lipids, four soluble N-ethylmaleimide-sensitive factor attachment protein recep
188 ies of membrane fusion mediated by soluble N-ethylmaleimide-sensitive factor attachment protein recep
189 C2 domains, and the neuronal core soluble N-ethylmaleimide-sensitive factor attachment protein recep
190 eins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein recep
191 ion is mediated by the concerted action of N-ethylmaleimide-sensitive factor attachment protein recep
193 ctivity on one of the three SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
194 s derived from target- and vesicle-soluble N-ethylmaleimide-sensitive factor attachment protein recep
195 ularly involving small G proteins, soluble N-ethylmaleimide-sensitive factor attachment protein recep
196 a Gbetagamma interaction with the soluble N-ethylmaleimide-sensitive factor attachment protein recep
197 /Munc18 (SM) proteins bind cognate soluble N-ethylmaleimide-sensitive factor attachment protein recep
198 d botulinum neurotoxin C to cleave soluble N-ethylmaleimide-sensitive factor attachment protein recep
199 th purified yeast vacuolar SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein recep
200 Yeast vacuole fusion requires soluble N-ethylmaleimide-sensitive factor attachment protein recep
201 tter release requires three SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
202 e transport of the plasma membrane soluble N-ethylmaleimide-sensitive factor attachment protein recep
203 ulinum neurotoxins cleave specific soluble N-ethylmaleimide-sensitive factor attachment protein recep
204 do not contain the t-SNARE (target-soluble N-ethylmaleimide-sensitive factor attachment protein recep
205 and vacuole protein sorting), and soluble N-ethylmaleimide-sensitive factor attachment protein recep
207 ediated selective concentration of soluble N-ethylmaleimide-sensitive factor attachment protein recep
208 elta) for the Tlg2 target membrane-soluble N-ethylmaleimide-sensitive factor attachment protein recep
210 inds to and cleaves syntaxin 17, a soluble N-ethylmaleimide-sensitive factor attachment protein recep
211 specific fusion proteins [such as soluble N-ethylmaleimide-sensitive factor attachment protein recep
212 c13-4 is a Ca(2+)-dependent SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
213 y GTPases, their effector tethers, soluble N-ethylmaleimide-sensitive factor attachment protein recep
216 and activation of (phago)lysosomal soluble N-ethylmaleimide-sensitive factor attachment protein recep
217 ane protein 4 (VAMP4), a vesicular soluble N-ethylmaleimide-sensitive factor attachment protein recep
219 ich, in turn, interacts with the lysosomal N-ethylmaleimide-sensitive factor attachment protein recep
222 vitro and to separate the roles of soluble N-ethylmaleimide-sensitive factor attachment protein recep
224 l by cooperating with the neuronal soluble N-ethylmaleimide-sensitive factor attachment protein recep
225 release requires the formation of soluble N-ethylmaleimide-sensitive factor attachment protein recep
226 interact with the vacuolar SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein recep
228 er with syntaxin-3 and syntaxin-1A soluble N-ethylmaleimide-sensitive factor attachment protein recep
229 component of the synaptic vesicle soluble N-ethylmaleimide-sensitive factor attachment protein recep
232 ituted with the target (t)-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein recep
233 it a polarized distribution of the soluble N-ethylmaleimide-sensitive factor attachment protein recep
234 e Dawley rats, 4 dominant-negative soluble N-ethylmaleimide-sensitive factor attachment protein recep
235 ted by the formation of functional soluble N-ethylmaleimide-sensitive factor attachment protein recep
236 the Rab GTPase Ypt7p, four SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein recep
237 anosine 5'-3-O-(thio)triphosphate, soluble N-ethylmaleimide-sensitive factor attachment protein, and
238 protein receptor (SNARE) proteins soluble N-ethylmaleimide-sensitive factor attachment protein-25 (S
239 590 (a predicted alpha-SNAP [alpha-soluble N-ethylmaleimide-sensitive factor attachment protein]).
240 le of the fusion proteins, SNAREs (soluble N-ethylmaleimide-sensitive factor attachment proteins), in
241 ine triphosphate-binding proteins, soluble N-ethylmaleimide-sensitive factor attachment proteins, and
243 ytosis relies on assembly of three soluble N-ethylmaleimide-sensitive factor attachment receptor (SNA
244 ysosomes for degradation following soluble N-ethylmaleimide-sensitive factor attachment receptor (SNA
246 Syntaxin 1a is a plasma membrane soluble N-ethylmaleimide-sensitive factor attachment receptor prot
247 ns homologous to eukaryotic SNARE (soluble N-ethylmaleimide-sensitive factor attachment receptor) dom
248 yt 1 using a reconstituted, SNARE (soluble N-ethylmaleimide-sensitive factor attachment receptor)-med
249 tail-anchored proteins, including soluble N-ethylmaleimide-sensitive factor attachment receptors (SN
250 ence in the NSF gene, encoding the protein N-Ethylmaleimide-Sensitive Factor essential for synaptic v
251 biting the binding of SNAREs to Sec18p, an N-ethylmaleimide-sensitive factor homologue responsible fo
252 ing the trimerized alphaSNAP, we find that N-ethylmaleimide-sensitive factor hydrolyzes 10 ATP molecu
254 e-resident syntaxin-like glutamine-soluble N-ethylmaleimide-sensitive factor protein attachment prote
255 ;5 are regulated by the SNARE (for soluble N-ethylmaleimide-sensitive factor protein attachment prote
258 complex is disassembled by the AAA-ATPase N-ethylmaleimide-sensitive factor that requires the cofact
260 an essential component of the soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein rece
262 attachment protein, and NSF is defined as N-ethylmaleimide-sensitive factor) complexes catalyze syna
263 SNARE bundles are reactivated by hexameric N-ethylmaleimide-sensitive factor, vesicle-fusing ATPase (
264 SNX27-independent recycling may involve N-ethylmaleimide-sensitive factor, which binds both PDZ in
265 tructures, and here we examine the role of N-ethylmaleimide-sensitive factor-activating protein recep
266 al membrane transporters, ATPases, soluble N-ethylmaleimide-sensitive factor-activating protein recep
267 uring synaptic vesicle fusion, the soluble N-ethylmaleimide-sensitive factor-attachment protein recep
269 enetic approach to identify target soluble N-ethylmaleimide-sensitive factor-attachment protein recep
272 cular synapses through cleavage of soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein
273 NT-CT interaction is further disrupted by N-ethylmaleimide-sensitive fusion ATPase (NSF), which asso
274 ment of Ca(2+) for the assembly of soluble N-ethylmaleimide-sensitive fusion attachment protein recep
275 sicle (DCV) exocytosis is a SNARE (soluble N-ethylmaleimide-sensitive fusion attachment protein recep
276 lates SNAP-23, the target membrane soluble N-ethylmaleimide-sensitive fusion factor attachment protei
277 We identified a direct interaction between N-ethylmaleimide-sensitive fusion protein (NSF), an ATPase
280 integral membrane proteins called soluble N-ethylmaleimide-sensitive fusion protein attachment prote
282 mbrane associated proteins SNAREs (soluble N-ethylmaleimide-sensitive fusion protein attachment prote
284 s between synaptotagmin and SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment recep
285 dependent on GluA2 not GluA1, sensitive to N-ethylmaleimide-sensitive fusion protein interaction, and
286 and specific cleavage of neuronal soluble N-ethylmaleimide-sensitive fusion protein-attachment prote
287 sicles in the brain harbor several soluble N-ethylmaleimide-sensitive-factor attachment protein recep
288 ery ECs, depletion of Galpha12 and soluble N-ethylmaleimide-sensitive-fusion factor attachment protei
290 , glutathione was derivatized in-situ with N-ethylmaleimide to block the cysteine residue and to enha
291 g of untreated, Cytochalasin B treated and N-Ethylmaleimide treated MCF-7 breast cancer cells demonst
294 either functionally impaired by trypsin or N-ethylmaleimide treatments or with protein-free liposomes
295 e nonspecific small molecule DUB inhibitor N-ethylmaleimide was 16.2+/-3.2 muM and can be used as a q
297 minoethyl methanethiosulfonate, but not by N-ethylmaleimide, was fully protected in the presence of s
298 the kinase inhibitor staurosporine and by N-ethylmaleimide, whereas KCC2(WT), KCC2(T934A), and KCC2(
299 ion of its only free cysteine residue with N-ethylmaleimide), which causes significant reduction in i
300 ith either 20mM added NaCl (WPI+NaCl), 5mM N-ethylmaleimide (WPI+NEM) or 20mM added NaCl and 5mM NEM