ライフサイエンスコーパス: exon inclusion

1 xon skipping, at one locus the Alu increases exon inclusion.

2 ng Nf1 exon 23a were manipulated to increase exon inclusion.

3 ping, and a reduction in PTB increased alpha-exon inclusion.

4 tisense oligonucleotide also increased alpha-exon inclusion.

5 s with G-quadruplex-forming capacity promote exon inclusion.

6 T MSE and positively regulates MSE-dependent exon inclusion.

7 f SRp40 in L6 cells mimicked insulin-induced exon inclusion.

8 SEs) are important cis elements required for exon inclusion.

9 -affinity site for U2AF65 strongly inhibited exon inclusion.

10 ternative exon 5 and promote muscle-specific exon inclusion.

11 t of a complex that regulates MSE3-dependent exon inclusion.

12 city while keeping G tracts intact abrogates exon inclusion.

13 nce that was specifically required for alpha-exon inclusion.

14 the exon were found to be required for alpha-exon inclusion.

15 element alone can at least partially support exon inclusion.

16 ive exon were sufficient for muscle-specific exon inclusion.

17 he native repeats act to enhance alternative exon inclusion.

18 me to select for exon sequences that enhance exon inclusion.

19 o coordinate splice site pairing and enforce exon inclusion.

20 ly 350 base pairs resulted in enhanced alpha-exon inclusion.

21 icing enhancer (ESE) in exon 3 that promotes exon inclusion.

22 to mediate the embryonic splicing pattern of exon inclusion.

23 del gene and are associated with alternative exon inclusion.

24 enhances Sam68 acetylation and CD44 variant exon inclusion.

25 minus is important to repress UNC13A cryptic exon inclusion.

26 further support for their role in promoting exon inclusion.

27 gregation of TDP-43, associated with cryptic exon inclusion.

28 antisense-oligonucleotide drugs that promote exon inclusion.

29 iversity primarily by repressing alternative exon inclusion.

30 cts with these enhancers to promote cassette exon inclusion.

31 get transcripts, leading to misregulation of exon inclusion.

32 n removal to ultimately activate alternative exon inclusion.

33 ch likely allows splice site recognition and exon inclusion.

34 variable exon and causes increased variable exon inclusion.

35 RBPs are combinatorially required for sad-1 exon inclusion.

36 ts in FD lymphoblast cell lines by improving exon inclusion.

37 the enhancer, hnRNP L unexpectedly activates exon inclusion.

38 r eliminates the ability of Mbnl1 to promote exon inclusion.

39 D45 splicing regulatory elements and repress exon inclusion.

40 ences RNAPII occupancy patterns and promotes exon inclusion.

41 easibility of screening for drugs that alter exon inclusion.

42 tive 5' splice site selection or alternative exon inclusion.

43 1 in different cells determine the degree of exon inclusion.

44 to induce exon skipping but also to promote exon inclusion.

45 AY cluster enhanced spliceosome assembly and exon inclusion.

46 eak 5' splice site was capable of repressing exon inclusion.

47 hat augments the enhancer function to ensure exon inclusion.

48 tively active Akt2 kinase promotes PKCbetaII exon inclusion.

49 site on a nuclear splicing protein promoting exon inclusion.

50 tronic elements, resulting in enhancement of exon inclusion.

51 ction of the mutated SRp40 attenuated betaII exon inclusion.

52 splicing minigene revealed defective betaII exon inclusion.

53 ther in vitro or in vivo, leads to increased exon inclusion.

54 e) to determine the levels of TDP-43 cryptic exon inclusion across hundreds of thousands of publicly

55 iation between genome sequence variation and exon inclusion across the transcriptome, we report that

56 op candidates to identify potent and compact exon inclusion activation domains for splicing modulatio

57 Rp55 was also demonstrated to play a role in exon inclusion after the removal of intronic splicing si

58 report, we show that insulin regulates this exon inclusion (alternative splicing) via the phosphatid

59 identify synonymous positions important for exon inclusion, an alignment of organisms filtered based

60 between distant splice sites for control of exon inclusion and allows removal of a 74 kb intron as a

61 NPM binding to its targets prevents aberrant exon inclusion and backsplicing events.

62 (p.D107D), has a similar molecular defect of exon inclusion and causes X-linked mental retardation He

63 eted for SMAR1 showed increased CD44 variant exon inclusion and concomitant metastatic propensity, co

64 is also necessary and sufficient for Agrin Z exon inclusion and downstream Lrp4-mediated AChR cluster

65 We show that simultaneous exon inclusion and exclusion can be induced at distinct

66 nstrated programmable U snRNA guide-targeted exon inclusion and exclusion.

67 We found that SRSF10 promoted both exon inclusion and exclusion.

68 other switches these fluorescent products of exon inclusion and exclusion.

69 Both exon inclusion and exon skipping were found to post-tran

70 negative isoform suppressing poison cassette exon inclusion and instead promoting the retention of fl

71 nd alternative splicing, frequently altering exon inclusion and intron retention in ways not predicte

72 iverse splicing defects, such as alternative exon inclusion and intron retention, were characterized

73 y of this sequence is sufficient for in vivo exon inclusion and is the binding site for the known bri

74 r of this network, RBM39, repressed cassette exon inclusion and promoted intron retention within mRNA

75 h hnRNP H and hnRNP F as being repressors of exon inclusion and suggest that Fox proteins enhance the

76 of an optimal splice site does not guarantee exon inclusion and that the best predictor for exon reco

77 ximal region of exon 2 that facilitates both exon inclusion and Vif expression.

78 characterizes the ability of RBPs to induce exon inclusion and yields new molecular parts for progra

79 e preserve protein reading-frame in both the exon-inclusion and exon-skip splice forms.

80 coding the adhesion receptor CD44, promoting exon inclusion, and decreasing the overall level of CD44

81 represented cassette exon skipping, "poison" exon inclusion, and intron retention, phenocopying well-

82 rovide evidence that, in addition to cryptic exon inclusion, APA changes are a new facet of TDP-43 pa

83 cell-based assays, confirmed light-dependent exon inclusion as well as an increase in the target func

84 h LY294002 blocked insulin-induced PKCbetaII exon inclusion as well as phosphorylation of SRp40.

85 nic intermediate methylation correlates with exon inclusion at a level between that of fully methylat

86 e to the SMN2 exon 7 splice region, inducing exon inclusion at ligand doses ~500-fold lower than thos

87 cid SequenceS), to assess the criticality of exon inclusion based solely on information contained in

88 These polymorphisms potentiate cryptic exon inclusion, both in cultured cells and in brains and

89 cores were shown to correlate with levels of exon inclusion, both in vitro and in vivo.

90 demonstrated that SRp20 and SF2/ASF increase exon inclusion but that CUG-BP1 causes exon skipping.

91 Our results suggest that CUGBP2 promotes exon inclusion by a novel mechanism in which CUGBP2 dire

92 amily of proteins has been shown to activate exon inclusion by binding intronic G triplets.

93 racterized RNA binding proteins that promote exon inclusion by binding to exonic splicing enhancers (

94 We conclude that CUGBP2 activates exon inclusion by forming direct interactions with compo

95 the first example of positive regulation of exon inclusion by PTB.

96 es suggest that accurate regulation of micro-exon inclusion by RBFOX proteins and PTBP1 plays an impo

97 ovide rapid, scalable and robust readouts of exon inclusion changes and used these to evaluate 718 hu

98 cent divergent domain activate MSE-dependent exon inclusion demonstrating an unusual functional redun

99 Increased exon inclusion depends on the number, sequence and chemi

100 TDP-43 has emerged as a repressor of cryptic exon inclusion during pre-mRNA splicing, but a role for

101 ction of TDP-43 is as a repressor of cryptic exon inclusion during RNA splicing(2-4).

102 analysis identified a significant alternate exon inclusion event between exons 6 and 7 in CYP2C19 fo

103 F9 in normal tissues, and we uncover a novel exon inclusion event in RPS6KA6 that only occurs in two

104 Cytoskeletal and ECM transcripts subject to exon inclusion events included vinculin (VCL), tenascin

105 vents, including 3'UTR extension, as well as exon inclusion/exclusion impacting on protein kinase and

106 on use in the microarray data, clustering of exon inclusion/exclusion patterns across all Immunologic

107 measurements, such as the joint analysis of exon inclusion/exclusion reads to model alternative cass

108 ulated splicing events can be categorized as exon inclusion, exon exclusion, splicing factor up-regul

109 m or downstream ISS elements increased alpha-exon inclusion from 10% up to 70% in vivo.

110 and PCBP1-dependent alternative splicing and exon inclusion from a CD44 minigene.

111 onic element of MAG and modulate alternative exon inclusion from a MAG minigene reporter.

112 used to characterize splicing enhancers for exon inclusion from a pool of beta-globin-based three ex

113 arks characteristic of active promoters, and exon inclusion in a small but well-defined class of exon

114 ernative 5' splicing also enhance (suppress) exon inclusion in alternative 3' or cassette exon splici

115 Intriguingly, motifs that enhance (suppress) exon inclusion in alternative 5' splicing also enhance (

116 n of RNPS1 with p54 cooperatively stimulated exon inclusion in an ATP synthase gamma-subunit pre-mRNA

117 T that are both necessary and sufficient for exon inclusion in embryonic muscle.

118 rection of FGFR1 gene splicing to >90% alpha-exon inclusion in glioblastoma cells had no discernable

119 rs to monitor the combinatorial diversity of exon inclusion in individual transcripts.

120 c splicing enhancer (ESE) required for alpha-exon inclusion in JEG3 cells.

121 ions in these elements prevent activation of exon inclusion in muscle cells but do not affect the def

122 Preventing cytosine-rich exon inclusion in mutant KRAS/p53 PDACs decreases tumor

123 (ESE), which together determine the level of exon inclusion in naive cells.

124 sites that bind nSR100 to potently activate exon inclusion in neural cells while weakening 3' splice

125 cells but do not affect the default level of exon inclusion in nonmuscle cells.

126 SR proteins are well known to promote exon inclusion in regulated splicing through exonic spli

127 Global analysis of decoy exon inclusion in SFSWAP and UPF1 double knockdown cells

128 t other SR proteins, directly mediated EIIIB exon inclusion in the fibronectin transcript.

129 r PTBP1, and this complex increased cassette exon inclusion in the mRNA encoding the chromatin remode

130 This approach allows us to detect exon inclusion in the primary transcripts themselves, ra

131 K1) is a regulator of Tra2beta1 and promotes exon inclusion in the survival motor neuron gene 2 (SMN2

132 filing reveals that ZMAT3 directly modulates exon inclusion in transcripts encoding proteins of diver

133 Thus, RBM10 loss generates exon inclusion in transcripts regulating ECM-cytoskeleta

134 Recombinant hnRNP-L functions to repress exon inclusion in vitro in an ESS1-dependent manner.

135 he binding of U2AF eliminated enhancement of exon inclusion in vivo and exon polyadenylation in vitro

136 onin T (cTNT) alternative exon 5 and promote exon inclusion in vivo and in vitro.

137 combinant PurH with functional activation of exon inclusion in vivo.

138 ancer pyrimidine tract is functional in that exon inclusion increases when in vivo levels of PTB incr

139 ous nuclear ribonucleoprotein A1 facilitates exon inclusion, increasing this ratio.

140 RNP A2B1 bind UNC13A RNA and repress cryptic exon inclusion, independently of TDP-43.

141 ntarity using compensatory mutations rescues exon inclusion, indicating that the elements act through

142 text with other sequence elements created by exon inclusion involved in affecting mRNA stability.

143 hese results suggest that although the alpha-exon inclusion is actively repressed in glioblastomas, t

144 ty of Nova to enhance or repress alternative exon inclusion is dependent on the position of the Nova-

145 he mechanism of MBNL1-mediated activation of exon inclusion is unknown.

146 velocity, whereas knockdown of TNC and CD44 exon inclusion isoforms reduced invasiveness.

147 RNA-binding probes to determine alternative exon inclusion level in aortic endothelial cells.

148 are high in Nf1 23aIN/23aIN cells, where the exon inclusion level remains high, but Ras activation is

149 utionarily optimized to support a particular exon inclusion level.

150 became stronger as a function of decreasing exon inclusion level: for alternatively spliced exons th

151 We established cryptic exon inclusion levels across a variety of human cells an

152 We found that predicted exon inclusion levels for the EF-hand motifs and for Ca2

153 NA variants produced smaller fluctuations of exon inclusion levels than random exonic substitutions,

154 e introduce SpliceTrap, a method to quantify exon inclusion levels using paired-end RNA-seq data.

155 e, and the purine-rich element also supports exon inclusion mediated through the downstream 5' splice

156 selection for in vitro splicing via internal exon inclusion, new consensus motifs and score matrices

157 gnificantly, insulin regulation of PKCbetaII exon inclusion occurred in the absence of cell growth an

158 CELF6 activates exon inclusion of a cardiac troponin T minigene in trans

159 purine residues were critical for repressing exon inclusion of a chimeric splicing reporter.

160 five of these proteins specifically activate exon inclusion of cardiac troponin T minigenes in vivo v

161 ng RNAs in vitro and activated MSE-dependent exon inclusion of cTNT minigenes in vivo.

162 There was a 6- to 14-fold decrease in exon inclusion on ablation of SRp55.

163 binatorial SREs which may be responsible for exon inclusion or exclusion across tissues.

164 Thus in most cases the pattern of exon inclusion or exclusion correlated with stronger or

165 and 3' untranslated regions (UTRs), promotes exon inclusion or exclusion in a context-dependent manne

166 an RNA-binding protein that promotes either exon inclusion or exclusion.

167 or Ca2+ and other divalent metals confer the exon inclusion order that mirrors the Irving-Williams af

168 P-43 is the main repressor of UNC13A cryptic exon inclusion, other hnRNPs contribute to its regulatio

169 e associated variant induces allele-specific exon inclusion (P = 0.0024).

170 as preneoplasias exhibited a more restricted exon inclusion pattern.

171 a developmentally regulated switch such that exon inclusion predominates in embryonic, but not adult,

172 rence, that it is involved in the higher EDA exon inclusion rate in endometrium.

173 In particular, we observed changes in exon inclusion rates and increased presence of intronic

174 m and gene expression as well as the derived exon inclusion rates.

175 w that BRIE yields reproducible estimates of exon inclusion ratios in single cells and provides an ef

176 ecially for accurate quantification of local exon-inclusion ratios from RNA-seq data.

177 y, robustness and reliability in quantifying exon-inclusion ratios.

178 Because SRSF2 promotes exon inclusion, reduced SRSF2 expression would mean that

179 mutations are observed to occur, none of the exon inclusion reducing mutants was found in the filtere

180 Cryptic exon inclusion reflects loss of function of TDP-43, and

181 Analysis of exon inclusion revealed an absence of brain-specific mic

182 cific splicing enhancers (MSEs) that promote exon inclusion specifically in embryonic striated muscle

183 They both stimulate exon inclusion, SRSF1 by binding to exonic splicing enha

184 ynonymous mutations across the exon decrease exon inclusion, suggesting that nucleotide identity acro

185 fluorescent reporter for cellular assays of exon inclusion that can accommodate nearly any cassette

186 re that the selected enhancer motif promotes exon inclusion through specific interaction with SRp30.

187 midines in the ESE resulted in a switch from exon inclusion to exon skipping in vivo and abolished bi

188 ound hatching coincided with the switch from exon inclusion to exon skipping, suggesting that loss of

189 Knockdown of the VCL exon inclusion transcript in RBM10-null cells reduced ce

190 We demonstrate that Wt1 promotes exon inclusion using a Vegf-a minigene-based splicing as

191 tested, RT-PCR-mediated detection of cryptic exon inclusion was able to diagnose IBM with 84% sensiti

192 and nonneuronal cell lines, and the level of exon inclusion was measured by primer extension.

193 Increased exon inclusion was not sequence-specific as exon skippin

194 dentify RBPs that directly drive alternative exon inclusion, we developed tethered function luciferas

195 identify substances that promote alternative exons inclusion, we set up a high-throughput screen and

196 Insulin effects on exon inclusion were observed as early as 15 min after in

197 ose to the 5' splice site generally promoted exon inclusion, whereas binding near the 3' splice site

198 small nuclear ribonucleoprotein) binding and exon inclusion, whereas Nova binding to an intronic YCAY

199 inding to cassette exons was associated with exon inclusion, whereas the binding of SRSF10 within dow

200 major role in maintaining normal FGFR1 alpha-exon inclusion, which is subject to dominant intronic sp

201 redicted Nova's effect to inhibit or enhance exon inclusion, which led us to examine the relationship

202 sufficient for Nova-dependent enhancement of exon inclusion, which we term the NISE (Nova-dependent i

203 U)-binding splicing factor 60 (PUF60) drives exon inclusion within proliferation-associated transcrip

204 tified pyrvinium pamoate as a drug-promoting exon inclusion without editing.

205 ation of the branchpoint caused loss of mini-exon inclusion without loss of splicing enhancement, sho