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1 ally removed with uracil DNA glycosylase and exonuclease III.
2 from different species and a comparison with exonuclease III.
3 ately 100-fold less efficient than repair by exonuclease III.
4 edly, they alter the cleavage specificity of exonuclease III.
5 scherichia coli DNA polymerase I, and E.coli exonuclease III.
6 phodiesters were protected from digestion by exonuclease III.
7 '-5' linkage itself was poorly hydrolyzed by exonuclease III.
8 ded double strand, which is then digested by exonuclease III.
9 to the location of the extra alpha-helix in exonuclease III.
10 the metal ion dependence of Escherichia coli exonuclease III, 3'-5'-exonuclease and exoribonuclease H
11 y enhanced the dCTPalphaB resistance towards exonuclease III (5-Et-dCTPalphaB >5-Me-dCTPalphaB >dCTPa
13 III-inactive protruding 3' terminus into an exonuclease III-active blunt end, triggering the digesti
14 9 degrees C/s ramp rate to support efficient Exonuclease III activity during signal amplification.
15 sensitive, and reproducible method based on Exonuclease III-aided target recycling technique applied
16 acids, comprising the 10 amino acids of the exonuclease III alpha-helix flanked by a glycine rich re
18 lethality caused by a combined deficiency of exonuclease III and dUTPase, which has been attributed t
20 tant cells lack activity at the positions of exonuclease III and endonuclease IV but retain activity
23 two Escherichia coli 3'-phosphodiesterases, exonuclease III and endonuclease IV, are readily detecte
25 , uracil DNA glycosylase-deficient, ung-, or exonuclease III and endonuclease IV-deficient, xth-nfo-)
27 escribe a generalizable method that utilizes exonuclease III and exonuclease I to interrogate the bin
28 repair proteins, including Escherichia coli exonuclease III and human APE, that repair oxidative and
29 bstrates by micrococcal nuclease mapping and exonuclease III and hydroxyl radical footprinting reveal
30 ization of the structure of AQ-157TG NCPs by Exonuclease III and hydroxyl radical footprinting, we co
31 igestion differs significantly between WRNp, exonuclease III and Klenow, indicating that each exonucl
32 ects of these linkages on the dsDNA specific exonuclease III and on the ssDNA specific mung bean nucl
33 ootprint of RNAP II arrested at a CPD, using exonuclease III and T4 DNA polymerase's 3'-->5' exonucle
34 ncipal replicative helicases or proofreading exonucleases; (iii) apparently orthologous but poorly co
35 rocessing enzymes such as endonuclease IV or exonuclease III are not absolutely required for repair o
37 nzymes, such as Escherichia coli RNase H and exonuclease III, are known to use metal cofactors in the
40 ical changes, in situ DNA end labeling, post-exonuclease III BrdUrd labeling, and DNA fragmentation.
42 Introduction of target sequence induces the exonuclease III catalyzed probe digestion and generation
51 h DNase I and 1,10-phenanthroline-copper and exonuclease III digestions showed that ecteinascidin 743
55 leic acid self-assembly circuitry and enzyme exonuclease III (Exo III) for the differentiation of sin
57 ified optical aptasensor system based on the Exonuclease III (Exo III) recycling of the VEGF analyte
59 AP endonucleases endonuclease IV (Endo IV), exonuclease III (Exo III), and Ape1 on the reaction kine
60 of homology to its prokaryotic counterpart, exonuclease III (Exo III), except for the amino terminus
61 ive detection of streptomycin (STR) based on Exonuclease III (Exo III), SYBR Gold and aptamer complim
62 multifunctional dumbbell probe can initiate exonuclease III (Exo III)-aided target recycling amplifi
64 -amino-acid domain of SNIP is related to the exonuclease III (ExoIII) domain of the 3'-->5' proofread
66 und that a combined treatment with MNase and exonuclease III (exoIII) overcomes MNase sequence prefer
68 which shares homology with Escherichia coli exonuclease III (ExoIII), is the major abasic endonuclea
69 , digests 5'-3', as well as Escherichia coli exonuclease III (ExoIII), which digests 3'-5', could sub
70 We have developed a novel DNA assay based on exonuclease III (ExoIII)-induced target recycling and th
72 roteins form a distinct subfamily within the exonuclease III (ExoIII)/Ape1/Apn2 family of proteins.
74 in vitro suggests that this property of the exonuclease III family of AP endonucleases is remarkably
77 trations, and is a specific inhibitor of the exonuclease III family of enzymes to which APE1 belongs.
79 s with a general view in the literature that exonuclease III favors double-stranded nucleic acid as a
81 e have examined the DNA binding affinity and exonuclease III footprint of the EcoKI type IA restricti
86 n resistance, lifetime, DNAseI footprinting, exonuclease III footprinting, permanganate footprinting
91 res extensive homology with Escherichia coli exonuclease III, has nuclease activity, and provides res
93 nd phosphatase activities of Esherichia coli exonuclease III have been quantitatively measured, offer
94 referential exodeoxyribonuclease activity of exonuclease III in combination with the difference in di
95 cribes a novel approach utilizing the enzyme exonuclease III in conjunction with 3'-terminated DNA mi
96 could also be obtained with purified E.coli exonuclease III in vitro, but a quantitative comparison
97 the presence of an additional alpha-helix in exonuclease III, in a position suggestive of interaction
98 n with the target DNA transforms the probe's exonuclease III-inactive protruding 3' terminus into an
99 ease-amplified DNA detection scheme in which exonuclease III is used to "recycle" target molecules, t
100 icking endonucleases or sequence independent exonuclease III, lambda exonuclease, RNase H, RNase HII,
101 mplate using a combination of digestion with exonuclease III, lambda exonuclease, RNAse T1, and treat
102 mino acids at Ser174, converts DNase I to an exonuclease III-like enzyme with DNA-repair properties.
105 in vitro reconstituted mononucleosomes using exonuclease III, micrococcal nuclease and restriction en
106 using a variety of nuclease probes including exonuclease III, micrococcal nuclease, DNase I, and rest
109 erwise identical DNA sequence was incised by exonuclease III or endonuclease IV approximately 6-fold
110 er significant nuclease resistance to either exonuclease III or mung bean nuclease, but unexpectedly,
111 ichia coli xthA mutants have also shown that exonuclease III participates in the repair of oxidative
112 fer assays are then used in conjunction with exonuclease III protection analysis to investigate the e
114 s, electrophoretic mobility shift assays and exonuclease III protection assays, we localized the basa
117 By mapping the breaks and by studies of the exonuclease III sensitivity of the broken ends, we concl
118 s faster than that recently observed for the exonuclease III surface hydrolysis of double-stranded DN
119 have suggested a role for Asn212 (Asn153 in exonuclease III, the bacterial homologue of HAP1) in sub
121 re, these results provide constraints on how exonuclease III-thiotriphosphate-polymerase combinations
122 orothioate linkages into DNA, and the use of exonuclease III to determine where those linkages have b
123 oxidized DNA by exploiting Escherichia coli exonuclease III to remove fragments containing direct st
124 units and the DNA could allow more access of exonuclease III to the DNA and account for the shorter f
128 was excised from a 2D gel and identified as exonuclease III using matrix-assisted laser-desorption i
132 PdG adducts were protected from digestion by exonuclease III, which was consistently arrested at posi
134 e AP endonuclease activities associated with exonuclease III (xth) and endonuclease IV (nfo), indicat
135 We have previously demonstrated that the two Exonuclease III (Xth) family members present within the