ライフサイエンスコーパス: exoribonuclease

1 ted the nsp10-driven activation of the nsp14 exoribonuclease.

2 al Mtb protein Rv2179c as a highly divergent exoribonuclease.

3 bonuclease, and Rrp6, a distributive 3'-->5' exoribonuclease.

4 polyribonucleotide polymerase or a 3'-to-5' exoribonuclease.

5 degraded by the nuclear exosome and a 5'-3' exoribonuclease.

6 otide phosphorylase (hPNPaseold-35), a 3',5'-exoribonuclease.

7 on, was also shown to be an Mn(2+)-dependent exoribonuclease.

8 subtilis yvaJ gene encodes a second 3'-to-5' exoribonuclease.

9 cterized as a Mn(2+)-dependent deadenylation exoribonuclease.

10 teract with SKI is degraded by 5'-to-3' XRN4 exoribonuclease.

11 gene regulation that involves both endo- and exoribonucleases.

12 (RppH), allowing access to both endo- and 5' exoribonucleases.

13 hosphorylated RNA that is a substrate for 5' exoribonucleases.

14 erfamily of nonspecific, 3'-->5', processive exoribonucleases.

15 n of the Rrp44p subunit that resembles other exoribonucleases.

16 cessivity factor to assist RNA maturation by exoribonucleases.

17 RNA, we used three Escherichia coli 3' to 5' exoribonucleases.

18 e and phylogenetic distribution of the known exoribonucleases.

19 adation in the 5' to 3' direction by the XRN exoribonucleases.

20 y via inhibition of the RNA-processing 5'-3' exoribonucleases.

21 RNA (sfRNA), by stalling the cellular 5'-3'-exoribonuclease 1 (XRN1) via structures located in their

22 The 5'-3'-exoribonuclease 2 (XRN2) degrades RNA to resolve R-loops

23 demonstrate that CARF associates with 5'-3' exoribonuclease 2 (XRN2), which plays a major role in bo

24 decrease coincided with an increase in 5'-3'-exoribonuclease 2 protein and alterations in DICER1 and

25 A human 3'-5'-exoribonuclease (3'hExo) has recently been identified an

26 e showed that subclass 1 SnRK2s, VCS, and 5' EXORIBONUCLEASE 4 (XRN4) are involved in regulating root

27 ncode the first known viral RNA proofreading exoribonuclease, a function that likely allowed expansio

28 nt and excess ncRNAs are largely degraded by exoribonucleases, a key characteristic of these RNAs is

29 tory mechanism affecting RNase R, a 3' to 5' exoribonuclease able to act on essentially all RNAs incl

30 family of enzymes, is a 3' to 5' hydrolytic exoribonuclease able to digest highly structured RNA.

31 presence of a 3'-phosphoryl group abolishes exoribonuclease action, it has no effect on the endoribo

32 Rrp6 is inhibited by a mutation in the Rrp44 exoribonuclease active site in 11-subunit nuclear exosom

33 unknown mechanism and compete with 3'-to-5' exoribonuclease activities in hen1.

34 s to the distributive or processive 3' to 5' exoribonuclease activities of Rrp6 or Rrp44, respectivel

35 Two other 3'-5'-exoribonuclease activities were also detected in the mit

36 e their processive and distributive 3'-to-5' exoribonuclease activities, respectively.

37 yrophosphohydrolase, decapping, and 5'-to-3' exoribonuclease activities.

38 ructural protein 14 (nsp14) encodes 3'-to-5' exoribonuclease activity (ExoN), which performs a proofr

39 nd its human homologs, DIS3 and DIS3L1, have exoribonuclease activity and bind to the core RNA exosom

40 s pyrophosphohydrolase, decapping, and 5'-3' exoribonuclease activity and functions as an important c

41 s accumulate in the absence of PNPase or its exoribonuclease activity and interact with PNPase.

42 Inhibition of 5' to 3' exoribonuclease activity and overexpression of an EJC di

43 tein 2 expressed in insect cells has a 3'-5' exoribonuclease activity and was therefore renamed RNA e

44 ncing and knockout approaches, along with 3'-exoribonuclease activity assays and additional biochemic

45 might be responsible for the acquisition of exoribonuclease activity by RNase BN.

46 for DXO is higher than the subsequent 5'-3' exoribonuclease activity for selected substrates.

47 estration mechanism for strict control of 3' exoribonuclease activity in the RE complex.

48 analyzed the role of Dcs1 in the control of exoribonuclease activity in vitro and propose that Dcs1

49 ns had reduced or were essentially devoid of exoribonuclease activity in vitro.

50 Finally, a stable structural barrier to exoribonuclease activity inhibited A-site cleavage when

51 ription, whereas the C domain contains 3'-5' exoribonuclease activity involved in suppressing interfe

52 doribonuclease and also demonstrate that its exoribonuclease activity is capable of functioning in vi

53 Rat1 exoribonuclease activity is stimulated by the protein Ra

54 A cytoplasmic 5'-3' exoribonuclease activity is therefore essential for yeas

55 xperimentally that citrate also inhibits the exoribonuclease activity of bacterial, eukaryotic and ar

56 vation of Rat1 by Rai1 and for the exclusive exoribonuclease activity of Rat1.

57 tion, purified Rai1p stabilized the in vitro exoribonuclease activity of Rat1p.

58 On the other hand, removal of the exoribonuclease activity of RNase BN in a cell lacking o

59 in the E. coli chromosome revealed that the exoribonuclease activity of RNase BN is not required for

60 The exoribonuclease activity of RNase BN is unnecessary beca

61 This impairs the 5'-3' exoribonuclease activity of RNase J1, increasing the hal

62 Loss of the exoribonuclease activity of RNase R was sufficient to in

63 cleavage become substrates for the 5' to 3'-exoribonuclease activity of the enzyme.

64 The exosome is named for the 3' to 5' exoribonuclease activity provided by a large C-terminal

65 Purified TREX1 displays robust exoribonuclease activity that degrades single-stranded,

66 have the largest RNA genomes, and encode an exoribonuclease activity that is required for high-fidel

67 p on mRNAs, and possesses distributive 5'-3' exoribonuclease activity toward 5'-monophosphate (5'-PO4

68 The aptamers resisted DXO's exoribonuclease activity, and in studies monitoring DXO'

69 Consistent with the DEDD fold, Rv2179c has exoribonuclease activity, cleaving the 3' single-strand

70 at of Bacillus subtilis RNase Z, which lacks exoribonuclease activity, revealed that RNase BN has a n

71 In vitro, TbRND exhibits 3' to 5' exoribonuclease activity, with specificity toward uridin

72 evaluated biochemically by monitoring nsp14 exoribonuclease activity.

73 virus RNA (sfRNA), a byproduct of host 5'-3' exoribonuclease activity.

74 its Mn(2+)-dependent ssRNA-specific 3'-to-5' exoribonuclease activity.

75 vides the sole source of processive 3'-to-5' exoribonuclease activity.

76 non-catalytic core and Rrp44, which inhibit exoribonuclease activity; and features of the Rrp44 exor

77 show that a noncoding RNA interacts with an exoribonuclease, altering its substrate specificity and

78 nd nsp16, stimulating their respective 3'-5' exoribonuclease and 2'-O-methyltransferase activities.

79 one suppressor, DIS3/RRP44, encodes a 3'-5' exoribonuclease and a member of the multisubunit exosome

80 eviously shown to act as both a distributive exoribonuclease and an endoribonuclease on model RNA sub

81 The exosome has both 3' to 5' exoribonuclease and endoribonuclease activity, and the a

82 As ascribed to the combined action of 5'->3' exoribonuclease and gene-specific RNA-binding proteins o

83 It is an exoribonuclease and integral component of the multienzym

84 lly and functionally interacts with the Rrp6 exoribonuclease and its associated cofactor Rrp47, the h

85 Mammalian PNPase exhibits exoribonuclease and poly(A) polymerase activities, and P

86 Polynucleotide phosphorylase (PNPase) is an exoribonuclease and poly(A) polymerase postulated to fun

87 cleotide phosphorylase (PNPASE), a 3' --> 5' exoribonuclease and poly-A polymerase, in the mitochondr

88 PNPase is a 3'-to-5' exoribonuclease and promotes the processive degradation

89 h nsp14, a bifunctional enzyme bearing 3'-5' exoribonuclease and RNA cap N7-guanine methyltransferase

90 gher-order degradation complex consisting of exoribonucleases and a decapping activity, which togethe

91 subtilis is accomplished by a combination of exoribonucleases and endoribonucleases.

92 nd on their catalytic properties, all of the exoribonucleases and their homologs have been grouped in

93 that concomitant loss of XRN4/EIN5, a 5'-3' exoribonuclease, and ABH1/CBP80, a subunit of the mRNA c

94 he terminal uridylyl transferase, U-specific exoribonuclease, and ligase activities of editing were u

95 , an endoribonuclease and processive 3'-->5' exoribonuclease, and Rrp6, a distributive 3'-->5' exorib

96 can be used to characterize newly-discovered exoribonucleases, and based on these motifs we correct s

97 rminus in conjunction with a generic 5'-->3' exoribonuclease; and (iii) remodels the structure of the

98 Exoribonucleases are vital in nearly all aspects of RNA

99 n identified Xrn1p, the cytoplasmic 5'-to-3' exoribonuclease, as a cofactor of RNAi in budding yeast.

100 se(old-35)), an evolutionary conserved 3',5'-exoribonuclease, as a gene up-regulated during both term

101 contrast, in Escherichia coli, a variety of exoribonucleases carry out final 3' maturation.

102 The exosome is a 3' to 5' exoribonuclease central to many cellular processes, incl

103 Ai) screen and identified a putative 3'-->5' exoribonuclease CG9247/nibbler essential for the generat

104 With greater resistance to exoribonuclease compared to the linear AR transcripts an

105 osome is an essential and conserved 3'-to-5' exoribonuclease complex that degrades or processes nearl

106 The RNA exosome is a multisubunit 3' to 5' exoribonuclease complex that participates in degradation

107 iral strategy to interfere with the 5'-to-3'-exoribonuclease component of the cytoplasmic RNA decay m

108 mes from yeast and humans contain two active exoribonuclease components, Rrp6p and Dis3p/Rrp44p.

109 Exosome complexes are 3' to 5' exoribonucleases composed of subunits that are critical

110 In gamma-proteobacteria, 3-'5' exoribonucleases comprise up to eight distinct enzymes.

111 We demonstrate that the 3' to 5' exoribonuclease decay pathway is a major contributor to

112 adducts in RNA directly by adduct-inhibited exoribonuclease degradation.

113 NPase(old-35)), a type I IFN-inducible 3'-5' exoribonuclease, degrades specific mRNAs and small nonco

114 unexpected source, pre-ribosomal RNA, in an exoribonuclease-dependent but DiGeorge syndrome critical

115 lyses strongly support a unified model of an exoribonuclease-dependent IFN suppression mechanism shar

116 Tyrosine fragments are generated in a DIS3L2 exoribonuclease-dependent manner and inhibit hnRNPA1-med

117 An exoribonuclease-dependent scavenger decapping activity w

118 ination factors cooperate with the conserved exoribonuclease Dhp1/Rat1/Xrn2, which couples pre-mRNA 3

119 ature of mRNAs that provides protection from exoribonuclease digestion and enhances translation.

120 We use exoribonuclease digestion and targeted RNA-sequencing to

121 an endoribonucleolytic cleavage followed by exoribonuclease digestion to generate 5'-mononucleotides

122 y the exosome complex containing the nuclear exoribonuclease Dis3.

123 te in cells and virions when the cytoplasmic exoribonuclease DIS3L2 and subunits of the RNA exosome a

124 ide an atomic view of a catalytically active exoribonuclease domain of LASV NP (LASV NP-C) in the pro

125 gh-resolution crystal structure of an active exoribonuclease domain of Tacaribe arenavirus (TCRV) NP.

126 hondrial RNA helicase that complexes with an exoribonuclease, DSS1, to function as an RNA degradosome

127 ity of their target protein, mouse decapping exoribonuclease (DXO).

128 Here, we show that a family of exoribonucleases encoded by the SMALL RNA DEGRADING NUCL

129 otic enzyme that belongs to a superfamily of exoribonucleases, endonucleases, and phosphatases.

130 that protects the RNA from degradation by 5' exoribonucleases, ensures efficient expression of viral

131 anine N7-methyltransferase (MTase) and 3'-5' exoribonuclease (ExoN) activities.

132 y syndrome CoV (SARS-CoV) nsp14 has 3'-to-5' exoribonuclease (ExoN) activity in vitro.

133 The nsp14 protein carries both exoribonuclease (ExoN) and (guanine-N7)-methyltransferas

134 leoproteins (NPs) contain a highly conserved exoribonuclease (ExoN) motif, through which LASV NP has

135 A mutants.IMPORTANCE Coronaviruses encode an exoribonuclease (ExoN) that is important for viral repli

136 the proofreading activity of the viral 3'-5' exoribonuclease (ExoN).

137 An L1 within ERI1 exoribonuclease family member 3 (ERI3) was found to asso

138 led that Y RNA tethers Ro60 to a ring-shaped exoribonuclease, forming a double-ringed RNP machine spe

139 We have purified a 3'-5'-exoribonuclease from mitochondrial extract of Leishmania

140 Most exoribonucleases function with cofactors that recognize

141 in the rnc3/4 double mutant, suggesting that exoribonucleases generated staggered ends in the absence

142 Four 3'-to-5' exoribonucleases have been identified in Bacillus subtil

143 Loss-of-function mutations in 3'-to-5' exoribonucleases have been implicated in hereditary huma

144 Escherichia coli RNase R, a 3' --> 5' exoribonuclease homologous to RNase II, was overexpresse

145 In this work, we report that the only exoribonuclease identified in M. genitalium, RNase R, is

146 RNase R, an Escherichia coli exoribonuclease important for degradation of structured

147 istence of another, as yet unknown, 3'-to-5' exoribonuclease in B. subtilis is suggested.

148 The 3'-to-5' exoribonuclease in coronavirus (CoV) nonstructural prote

149 s results, show that the normal role of this exoribonuclease in imaginal discs is to suppress the exp

150 family members in that it also can act as an exoribonuclease in vitro.

151 tholog Rsr (Ro sixty related) functions with exoribonucleases in 23S rRNA maturation.

152 orylase (cpPNPase) are the two known 3'-->5' exoribonucleases in Arabidopsis chloroplasts, and are in

153 veal the cooperative activity of two 3'-->5' exoribonucleases in chloroplast mRNA 3' end maturation,

154 of RNase R and how it is distinct from other exoribonucleases in E. coli.

155 from the RNR family of processive, 3' to 5' exoribonucleases in Escherichia coli.

156 Here, we compare the roles of these two exoribonucleases in HCV-infected cells and confirm that

157 Here, we dissect the roles of these two 5' exoribonucleases in restricting the replication of diffe

158 However, RNase R differs from other exoribonucleases in that it can by itself degrade RNAs w

159 RNase R is unusual among exoribonucleases in that, by itself, it can digest throu

160 c region (i) blocks both 5'-->3' and 3'--> 5 exoribonucleases in vitro; (ii) is sufficient to define

161 amount of RNase R, an important degradative exoribonuclease, increases 3-10-fold under a variety of

162 RNase R, an important exoribonuclease involved in degradation of structured RN

163 The cytoplasmic exosome, a complex of 3'-5' exoribonucleases involved in RNA degradation and process

164 The responsible exoribonuclease is Usb1, which removes nucleotides from

165 t of the exosome, the main cellular 3'-to-5' exoribonuclease, is a positive regulator of cuticular wa

166 cherichia coli RNase R, a processive 3'-to5'-exoribonuclease, is dramatically increased in response t

167 onstrate that RNase R, a processive 3'-to-5' exoribonuclease, is recruited to stalled ribosomes for t

168 rt that RNase R, a highly conserved 3' to 5' exoribonuclease, is required for the selective degradati

169 RNase II, a 3' to 5' processive exoribonuclease, is the major hydrolytic enzyme in Esche

170 horylase (PNPase), a 3'-to-5' phosphorolytic exoribonuclease, is thought to be the primary enzyme res

171 narily conserved complex of multiple 3'-->5' exoribonucleases, is responsible for a variety of RNA pr

172 We conclude that Xrn1 is the dominant 5' exoribonuclease mediating decay of HCV RNA and that miR-

173 OLD-35)), an evolutionarily conserved 3', 5' exoribonuclease mediating mRNA degradation, was first id

174 nated activities of ySuv3 helicase and yDss1 exoribonuclease (mtEXO), whereas in bacteria, RNA is deg

175 to the mitochondrion, a poly(A) specific 3' exoribonuclease, mtPARN, and a poly(A)binding protein, m

176 Decay of rpsO mRNA in a panel of 3'-to-5' exoribonuclease mutants was analyzed using a 5'-proximal

177 Genetically stable exoribonuclease mutants will allow direct testing of vir

178 RNAs undergo 3' end trimming by the 3'-to-5' exoribonuclease Nibbler (CG9247).

179 ein-protein interaction between the 3'-to-5' exoribonuclease Nibbler (Nbr) and Piwi that links Nbr ac

180 ucleoplasm trafficking, controlling 5'-to-3' exoribonuclease nucleolar levels and regulating rRNA pro

181 x that threads RNAs directly to the 3'-to-5' exoribonuclease of the cytoplasmic exosome, compensated

182 terestingly, other major 3'-to-5' processing exoribonucleases of E. coli, such as polynucleotide phos

183 terestingly, RNase BN acts as a distributive exoribonuclease on some substrates, releasing mononucleo

184 ning precursors, RNase BN acted as either an exoribonuclease or endoribonuclease depending on the nat

185 Seq) assay, we identify the poly(A)-specific exoribonuclease PDE12 as a major factor for the quality

186 Exoribonucleases play an important role in all aspects o

187 RNase R, a ubiquitous 3' exoribonuclease, plays an important role in many aspects

188 Although the 3' to 5' processive exoribonucleases, PNPase and RNase II, have long been co

189 es: polynucleotide phosphorylase, hydrolytic exoribonuclease, poly(A) polymerase, and CCA transferase

190 n reading frames (ORFs) potentially encoding exoribonucleases, poly(A) polymerases, and proteins know

191 rRNA profiles reveals that Rsr, the 3' to 5' exoribonuclease polynucleotide phosphorylase (PNP) and a

192 It has been demonstrated that the exoribonuclease polynucleotide phosphorylase (PNPase) fa

193 We report that Rsr and the exoribonuclease polynucleotide phosphorylase (PNPase) fo

194 sly found that the highly conserved 3'-to-5' exoribonuclease polynucleotide phosphorylase (PNPase) ha

195 Previous work has shown that the exoribonuclease polynucleotide phosphorylase (PNPase) is

196 Escherichia coli for tRNA(Leu5) in which the exoribonuclease polynucleotide phosphorylase (PNPase) re

197 We previously showed that the exoribonuclease polynucleotide phosphorylase (PNPase) wa

198 dosome are the endoribonuclease RNase E, the exoribonuclease polynucleotide phosphorylase (PNPase), a

199 t enzyme in organellar RNA metabolism is the exoribonuclease polynucleotide phosphorylase (PNPase), w

200 round in which expression of the chloroplast exoribonuclease polynucleotide phosphorylase was diminis

201 aintain cell viability in the absence of the exoribonuclease polynucleotide phosphorylase was markedl

202 enetic and biochemical interactions with the exoribonuclease polynucleotide phosphorylase, Rsr likely

203 nitase, a DEAD-box RNA helicase RhlB and the exoribonuclease polynucleotide phosphorylase.

204 sing three previously characterized 3'-to-5' exoribonucleases (polynucleotide phosphorylase [PNPase],

205 The activity of the phosphorolytic exoribonuclease, polynucleotide phosphorylase (PNPase),

206 ned the expression of pnp encoding the 3'-5'-exoribonuclease, polynucleotide phosphorylase, in Strept

207 a chloroplasts contain at least two 3' to 5' exoribonucleases, polynucleotide phosphorylase (PNPase)

208 in of Bacillus subtilis lacking two 3'-to-5' exoribonucleases, polynucleotide phosphorylase (PNPase)

209 -5p is more resistant to digestion by 3'->5' exoribonuclease polyribonucleotide nucleotidyltransferas

210 n vitro RNA degradation assays confirmed its exoribonuclease properties, and overexpression of hPNPas

211 this cleavage is rapidly degraded via the 5' exoribonuclease Rat1p which is thought to destabilize th

212 nucleotide phosphorylase (PNPase; a 3'-to-5' exoribonuclease) revealed a striking overexpression of t

213 ional degradosome component is the essential exoribonuclease RNase D, and its recognition site within

214 The subcellular localization of the exoribonuclease RNase II is not known despite the advanc

215 ration is efficient and requires Rsr and the exoribonucleases RNase PH and RNase II.

216 In Escherichia coli, the exoribonucleases RNase R and polynucleotide phosphorylas

217 molecular mass of 90.5 kDa, and exhibited an exoribonuclease (RNase R) activity.

218 ein (NPs) of all arenaviruses carry a unique exoribonuclease (RNase) domain that has been shown to be

219 leoproteins (NPs) revealed a conserved DEDDH exoribonuclease (RNase) domain that is important for typ

220 droxyl acylation analyzed by protection from exoribonuclease (RNase-detected SHAPE) should prove broa

221 Interestingly, the third 3'-to-5' processing exoribonuclease, RNase R of E. coli, which is cold induc

222 tected SHAPE that uses a processive, 3'-->5' exoribonuclease, RNase R, to detect covalent adducts in

223 2'-O-adducts block processivity of a 3'-->5' exoribonuclease, RNase R, to produce fragments that term

224 an RNA chaperone, CspA, and a cold-inducible exoribonuclease, RNase R.

225 In contrast to other exoribonucleases, RNase R can efficiently degrade highly

226 Here, we show that four 3'-->5'-exoribonucleases, RNases II, R, and PH, and polynucleoti

227 nit exosome core, while Rrp47 stabilizes the exoribonuclease Rrp6 and recruits Mtr4, but it is less c

228 yces cerevisiae, the nuclear/nucleolar 3'-5' exoribonuclease Rrp6 distinguishes the nuclear exosome f

229 9 subunits that associate with the 3' to 5' exoribonucleases Rrp6, and Rrp44/Dis3, a subunit that al

230 We further identified a 3' to 5' exoribonuclease, RRP6 (ribosomal RNA processing protein

231 NA precursor and that defects in the nuclear exoribonuclease Rrp6p enhance this effect.

232 The exoribonuclease Rrp6p is critical for RNA decay in the n

233 nuclease activity; and features of the Rrp44 exoribonuclease site that support its ability to degrade

234 However, although other Escherichia coli exoribonucleases stop several nucleotides downstream of

235 bacteria possess additional hydrolytic 3'-5' exoribonucleases such as RNase II, RNase R was found to

236 es not stimulate the activity of other 5'-3' exoribonucleases, such as Rat1, in vitro.

237 perties of the distributive U-specific 3'-5'-exoribonuclease suggest an involvement in the U-deletion

238 The Trypanosoma brucei exoribonuclease, TbDSS-1, has been implicated in multipl

239 We have identified a mitochondrial exoribonuclease, TbRND, whose expression is highly up-re

240 yme was found to be a single-strand-specific exoribonuclease that acts in the 3' to 5' direction in a

241 In summary, TbRND is a novel 3' to 5' exoribonuclease that appears to have evolved a function

242 ) nucleoprotein (NP) is the only known 3'-5' exoribonuclease that can suppress type I interferon (IFN

243 the 5' direction, suggesting that LigD is an exoribonuclease that cleaves the 3'-terminal phosphodies

244 otide phosphorylase (PNPase) is a processive exoribonuclease that contributes to messenger RNA turnov

245 RNase R is a 3' to 5' hydrolytic exoribonuclease that has the unusual ability to digest h

246 teins identified oligoribonuclease (Orn), an exoribonuclease that hydrolyzes two- to five-nucleotide-

247 Nase R appears to be the only known 3' to 5' exoribonuclease that is able to degrade through double-s

248 ase(old-35)) is a type I IFN-inducible 3',5' exoribonuclease that mediates mRNA degradation.

249 Eri1 is an evolutionarily conserved 3'-5' exoribonuclease that participates in 5.8S rRNA 3' end pr

250 Poly(A)-specific ribonuclease (PARN) is a 3'-exoribonuclease that plays an important role in regulati

251 show that human USB1 is a distributive 3'-5' exoribonuclease that posttranscriptionally removes uridi

252 mice deficient in Eri1, a conserved 3'-to-5' exoribonuclease that represses RNA interference, have a

253 RNase R is a processive, 3' to 5' hydrolytic exoribonuclease that together with polynucleotide phosph

254 Surprisingly, Nibbler, a 3'-5' exoribonuclease that trims 'long' mature miRNAs in AGO1,

255 In general, RNA degradation is via exoribonucleases that degrade RNA either from the 5' end

256 fies with the exosome, a complex of 3' to 5' exoribonucleases that is implicated in the processing of

257 nd unneeded ncRNAs are primarily degraded by exoribonucleases that rely on protein cofactors to ident

258 s catalyzed by specific subsets of endo- and exoribonucleases that together recycle RNA fragments int

259 RNA decapping enzymes hijack a host 5'-to-3'-exoribonuclease to evade antiviral innate immunity by li

260 a 275-nt RNA, which was then trimmed by a 3' exoribonuclease to the mature scRNA.

261 60 ortholog enhances the ability of 3'-to-5' exoribonucleases to degrade structured RNA during severa

262 While exoribonuclease treatment is widely used to degrade line

263 rs, including the mRNA-decapping complex and exoribonucleases, whereas another core factor, eIF4AIII/

264 acman/XRN1 is a highly conserved cytoplasmic exoribonuclease which degrades RNAs in a 5'-3' direction

265 f Saccharomyces cerevisiae encodes a 5'-->3' exoribonuclease which plays an essential role in yeast R

266 RNase R, was used to purify another 3'-to-5' exoribonuclease, which is encoded by the yhaM gene.

267 mber of the widely distributed RNR family of exoribonucleases, which are highly processive 3'-->5' hy

268 Nibbler (Nbr) is a 3'-to-5' exoribonuclease whose catalytic 3'-end trimming activity

269 host genes, including XRN1, encoding a 5'-3' exoribonuclease, whose absence led to an approximately 1

270 RNase R is a processive 3'-5' exoribonuclease with a high degree of conservation in pr

271 racting protein partners, OIP2, is a 3'-->5' exoribonuclease with a phosphorolytic activity that proc

272 cases and 2,816 controls implicated PARN, an exoribonuclease with no previous connection to telomere

273 transiently protect nascent ncRNA ends from exoribonucleases, with partner proteins that sequester t

274 uses (CoVs) are unique in encoding a 3'-->5' exoribonuclease within nonstructural protein 14 (nsp14-E

275 esults further confirm that SOX and the host exoribonuclease Xrn1 act in concert to elicit the rapid

276 these products was dependent on the 5'-->3' exoribonuclease Xrn1 and not the exosome.

277 hose isolated from plants, that the 5' to 3' exoribonuclease XRN1 can degrade elongated progenitor sg

278 virus (VACV) decapping enzymes and cellular exoribonuclease Xrn1 catalyze successive steps in mRNA d

279 f viral genomic RNA by the cytoplasmic 5'-3' exoribonuclease Xrn1 halted at the Xrn1-resistant RNA (x

280 litate the activity of the cytoplasmic 5'-3' exoribonuclease Xrn1 in eukaryotes.

281 The conserved multidomain exoribonuclease Xrn1 targets cytoplasmic RNA substrates

282 Furthermore, YTHDC2 recruits the 5'-->3' exoribonuclease XRN1 via Ankyrin repeats that are insert

283 The cytoplasmic 5' to 3' exoribonuclease XRN1 was responsible for the degradation

284 to accomplish this is to target the cellular exoribonuclease XRN1, because this enzyme is accessible

285 st-transcriptional mechanisms, involving the exoribonuclease Xrn1, to compensate the derepression of

286 radation factor Pat1/Mtr1 and with the 5'-3' exoribonuclease Xrn1.

287 fRNA results from stalling of the host 5'-3' exoribonuclease XRN1/Pacman on conserved RNA structures

288 from 5' decay mediated by the cytoplasmic 5' exoribonuclease, Xrn1.

289 ainst decay mediated by distinct cellular 5' exoribonucleases, Xrn1 and Xrn2.

290 member, was found to form a complex with the exoribonuclease-XRN1 to process miRNA maturation.

291 res the decapping enzyme Dcp1p, the 5'-to-3' exoribonuclease Xrn1p, and the three nonsense-mediated m

292 ilized by the deletion of the cytoplasmic 5' exoribonuclease (Xrn1p) or by inactivation of the cytopl

293 is homolog of the major yeast mRNA degrading exoribonuclease, Xrn1p.

294 by facilitating the interaction between the exoribonuclease XRN2 and select PDE transcripts.

295 diated by NKRF interaction with the 5'-to-3' exoribonuclease XRN2, a key coordinator of multiple pre-

296 horylated by Cdk9 was the 5'-to-3' "torpedo" exoribonuclease Xrn2, required in transcription terminat

297 We discovered that the 5'-3' exoribonuclease Xrn2, which plays a crucial role in the

298 port suggesting that a predominantly nuclear exoribonuclease, Xrn2, mediates the degradation of genot

299 tion of ETHYLENE-INSENSITIVE5 as the 5'-->3' exoribonuclease XRN4.

300 The 5'-->3' exoribonucleases (XRNs) comprise a large family of conse