ライフサイエンスコーパス: expression system

1 y regulates KCNQ2/3 channels in a simplified expression system.

2 pha-onocerin in the N. benthamiana transient expression system.

3 xpression of the designed vaccine in E. coli expression system.

4 ila KCNQ potassium channel in a heterologous expression system.

5 eption) were not affected in the HEK293 cell expression system.

6 adapted to other organisms with an inducible expression system.

7 Both vaccines were easily made via E. coli expression system.

8 to express functional PDE6 in a heterologous expression system.

9 ant proteins of A3F from an Escherichia coli expression system.

10 te odour-induced responses in a heterologous expression system.

11 ntly as a chimeric protein in a heterologous expression system.

12 to the tonoplast in a tobacco leaf transient expression system.

13 genes in a Daucus carota (carrot) protoplast expression system.

14 e, recombinant enzyme in the baculovirus/Sf9 expression system.

15 nt, in fly tissues using the UAS-Gal4 binary expression system.

16 s and cell display platforms in a eukaryotic expression system.

17 patients were evaluated using a prokaryotic expression system.

18 receptors to human odorants with the Xenopus expression system.

19 type I, were produced using a tobacco-based expression system.

20 flux using an in vitro cellular heterologous expression system.

21 y enhances its surface expression in HEK293T expression system.

22 tein function was assessed in a heterologous expression system.

23 in the malaria mosquito, AgGr25, in the ab1C expression system.

24 is of marineosins, using a marG heterologous expression system.

25 repeats) produced in the Lactococcus lactis expression system.

26 derived DCs (MDDCs) using a lentivirus-based expression system.

27 k P(o) is doubled by SCYL1 in a heterologous expression system.

28 ary human beta-cells and from a heterologous expression system.

29 L protein was expressed using a baculovirus expression system.

30 ic channels when expressed in a heterologous expression system.

31 produced in several E. coli strains with T7 expression system.

32 robic conditions with an arabinose-inducible expression system.

33 -chain produced recombinantly in a bacterial expression system.

34 menthol on beta2* nAChRs studied in the same expression system.

35 iving ciliate, Tetrahymena thermophila as an expression system.

36 l structures, and the lack of a heterologous expression system.

37 roduction requires an efficient heterologous expression system.

38 ased Bombyx mori nucleopolyhedrovirus bacmid expression system.

39 nts, using a dual RNA polymerase II promoter expression system.

40 fy transcription factor-based inducible gene expression systems.

41 ls in order to compare widely used mammalian expression systems.

42 localization of human ClC-4 in heterologous expression systems.

43 A11-tsC genetic context, we employed ectopic expression systems.

44 subtypes have been described in heterologous expression systems.

45 acted and purified from heterologous protein expression systems.

46 solely on protein derived from heterologous expression systems.

47 sing both constitutive and ethanol-inducible expression systems.

48 untered in the Golgi Apparatus in human cell expression systems.

49 w in both wide-type strains and heterologous expression systems.

50 ane proteins from prokaryotic and eukaryotic expression systems.

51 hat are rich in tyrosine codons in bacterial expression systems.

52 ancement over and above the most widely used expression systems.

53 proteins in functional forms in heterologous expression systems.

54 vaccine testing in a variety of heterologous expression systems.

55 n various signaling pathways in heterologous expression systems.

56 logy toolkit beyond traditional constitutive expression systems.

57 well studied of all proteins in heterologous expression systems.

58 gs on human cardiac currents in heterologous expression systems.

59 for the trafficking of Cx43 in heterologous expression systems.

60 parations, including native and heterologous expression systems.

61 nAChR-selective agonists using heterologous expression systems.

62 l consequences were explored in heterologous expression systems.

63 ble production of HTT in multiple eukaryotic expression systems.

64 hippocampal A-type currents in heterologous expression systems.

65 NOG using embryonic stem cells and bacterial expression systems.

66 ichia coli protein solubility in a cell-free expression system, 35 sequence-based properties are calc

67 To expand the versatility of the 2A expression system, a number of commonly used selectable

68 and eCPMV (RNA-free VLPs) produced from two expression systems (agrobacterium-based plant expression

69 of a robust Agrobacterium-mediated transient expression system allowed us to assemble each virion typ

70 We use a heterologous expression system and a hydrophilic interaction chromato

71 e changes were studied using a new cell-free expression system and a mass spectrometry-based assay fo

72 rm of IL-11 in an optimized Escherichia coli expression system and administered it as the aerosol in

73 xpression systems (agrobacterium-based plant expression system and baculovirus-insect cell expression

74 n SPCA1a/2 isoforms from a yeast recombinant expression system and compared their biochemical propert

75 reen for ROMK function, we developed a yeast expression system and discovered that yeast cells lackin

76 CXCR4 heteromerizes with PAR1 in the HEK293T expression system and in human primary pulmonary endothe

77 be coimmunoprecipitated with CXCR4 in a HeLa expression system and in human VSMC.

78 hysiological analyses using both an in vitro expression system and intact rodent hippocampal brain sl

79 Each droplet contains a cell-free expression system and is connected to its neighbor throu

80 ncoded cyanine dye as a ncAA in a eukaryotic expression system and opens the door for the analysis of

81 Using a cell-free expression system and pulse-proteolysis experiments, we

82 xpressed ATGYRA and ATGYRB2 in a baculovirus expression system and shown supercoiling activity of the

83 c antibodies to be produced in virtually any expression system and still maintain effector function.

84 torsinADeltaE, we developed a yeast torsinA expression system and tested the roles of ER chaperones

85 rda (Sf9) cells using a baculovirus-mediated expression system and the enzymatic activity and metabol

86 Using the Xenopus oocytes expression system and two microelectrode voltage-clamp,

87 bodies (mAbs) using both plant and mammalian expression systems and completed head-to-head in vitro a

88 In heterologous expression systems and cultured sensory neurons, exposur

89 s studies of DAT trafficking in heterologous expression systems and dissociated cultured neurons, stu

90 are poorly understood, although heterologous expression systems and mouse models have demonstrated al

91 spoIIID and sigK mutants using heterologous expression systems and RNA-Seq transcriptional profiling

92 man pathologies, the advances in recombinant expression systems and structural data retrieving, and t

93 12 different versions, differing by protein expression systems and tagging configurations.

94 ced enhancer tools combined with conditional expression systems and the PiggyBac-mediated DNA transpo

95 siveness in both heterologous and endogenous expression systems and whether this affects PTH secretio

96 h cells transduced with a DOX-inducible KLF4 expression system, and find KLF4 function inhibits PC3 g

97 of Kv2.1 mimicking apoptosis in a mammalian expression system, and protected cortical neurons from s

98 y expressed all 36 PvTRAgs using a cell-free expression system, and, for the first time, profiled the

99 evisiae (S. cerevisiae) Gpi8 using different expression systems, and examined its interaction with in

100 recombinant EDIII proteins and the different expression systems, and reached between 0.8 and 1.6 % of

101 ed against a number of commonly used E. coli expression systems, and shows tight basal control, preci

102 Csy4 and tRNA expression systems are almost twice as effective in indu

103 Cell-, tissue- or organ-specific inducible expression systems are powerful tools for functional ana

104 Tightly regulated gene expression systems are powerful tools to study essential

105 Inducible gene expression systems are vital tools for the advancement o

106 plastid DNA (ptDNA) and its associated gene expression system, as reported recently in several linea

107 rties render them incompatible with a single expression system, as well as by the semisynthesis of du

108 esent iTango, a light- and ligand-gated gene expression system based on a light-inducible split tobac

109 Here, we develop a compact shRNA (cshRNA) expression system based on retroviral microRNA (miRNA) g

110 Herein the distinct expression systems based on viral elements are analyzed.

111 ccine platform based on the baculovirus dual-expression system (BDES) was evaluated.

112 In heterologous expression systems, both the TMD and CR of PCDH15 have b

113 arrestin binding in an in vitro heterologous expression system but that also impairs receptor express

114 ractions in many cell types and heterologous expression systems, but interactions between alpha6beta4

115 shown to interact with AMPARs in artificial expression systems, but it is unknown whether Shisa7 has

116 Here we report a new transgenic expression system by combination of liver-specific expre

117 P1 was analyzed in the Xenopus laevis oocyte expression system by two-electrode voltage clamp and opt

118 ercomes the limitations of current inducible expression systems by enabling robust spatial and tempor

119 Plant viral expression systems can be used for rapid expression of p

120 l-free assays or phenotypic assays in native expression systems can uncover activation of additional

121 In heterologous expression systems, coexpression of NCC with I-1 increas

122 hich the two copies of NS5A from the various expression systems colocalize, the results point to NS3-

123 re functionally characterized in a transient expression system consisting of Agrobacterium-infiltrate

124 Perspectives on how viral expression systems could allow for the development of in

125 mical studies of recombinant Y3 from a yeast expression system demonstrated the protein is a unique G

126 ene switch system, a chemically induced gene expression system, demonstrated that induction of 9-cis-

127 Translation in a cell-free expression system demonstrates that diminished output fr

128 s, and that subunit linkage can overcome the expression system-dependent preferential assembly of fre

129 oration combined with an inducible knockdown expression system drives deficits in synaptic GABAA func

130 Using a heterologous GABA(B)R expression system, electrophysiology, and mutagenesis, w

131 Advances in transient mammalian expression systems enable high yields (>100 mg/liter) th

132 reconstituted via an optimized two-component expression system encoding the CRISPR-associated (Cas) p

133 er-optimized RNA for durable overexpression) expression system for achieving rapid RNA circularizatio

134 In this study, using an inducible expression system for an miRNA target message, we have d

135 ssly transition into a large-scale mammalian expression system for clinical production.

136 Plants are increasingly being used as an expression system for complex recombinant proteins.

137 e, we address this issue using a recombinant expression system for human dynein coupled to single-mol

138 d to a RMR motif and cloned into a bacterial expression system for protein production.

139 residues, based on an established functional expression system for PsN(2)OR in E. coli.

140 novel reporters and a doxycycline-regulated expression system for RPB1 In efforts to determine the m

141 Using an inducible expression system for the exosome cargo protein GPRC5B a

142 er can be applicable in establishing a novel expression system for the high-level production of forei

143 nt Anopheles gambiae densovirus (AgDNV) over-expression system for the major malaria vector Anopheles

144 We established a controlled expression system for the parallel functional analysis o

145 Here we present an efficient cell-free expression system for the site-specific incorporation of

146 The Q system is a repressible binary expression system for transgenic manipulations in living

147 for instance, those enabling transgenic GFP expression systems for in vivo visualization.

148 ince new molecular tools, including improved expression systems for proteins, protein and metabolic e

149 One of the most commonly used expression systems for the production of membrane protei

150 s novel system expands the number of E. coli expression systems for use in recombinant protein produc

151 (grams of protein per litre), cell-free gene expression systems from model bacteria, the development

152 f truncated hClC-Kb channels in heterologous expression systems fully explains the reduced basolatera

153 Toxicity studies in a prokaryotic dual-expression system grouped the enzymes into high- and low

154 The expression system had a strong effect on the properties

155 cleavage activity in a bacterial carotenoid expression system, had activity similar to lancelet BCO2

156 However, no efficient non-tagged apidaecin expression system has been reported, which is the limiti

157 utamate receptor function using heterologous expression systems has complicated the analysis of recep

158 Guanine constraint commonly imposed by gRNA expression systems has little effect on overall cleavage

159 Baculovirus mediated-insect cell expression systems have been widely used for producing h

160 full-length Pfs48/45 using different protein expression systems have not produced an antigen with sat

161 Binary expression systems have revolutionised genetic research

162 In 2 heterologous expression systems (HEK-293T cells and Xenopus laevis oo

163 To this end, using a conditional expression system, here we generated Plk1 transgenic mou

164 +) channel-mediated currents in heterologous expression systems; (ii) molecular dynamics simulations

165 d membrane fusion in a plasmid-based protein expression system, illustrating the importance of using

166 In this paper, we describe a transient expression system in Arabidopsis mesophyll protoplasts t

167 Upon engineering an inducible csrA expression system in B. burgdorferi, controlled hyperexp

168 Finally, we use a lentiviral expression system in cultured neurons, where we again fi

169 Thus, heterologous expression system in Dictyostelium cells provides mechan

170 hemical properties of these mutants using an expression system in Escherichia coli that produces homo

171 Using an inducible expression system in human HT1080 fibrosarcoma cells, we

172 We previously developed a transient expression system in Nicotiana benthamiana that allowed

173 We established a transient expression system in Nicotiana benthamiana to study deta

174 then expressed using the magnICON transient expression system in plants and their enzymatic properti

175 for a plastid genome and an associated gene expression system in Polytomella spp.

176 tissue-specific expression and an inducible expression system in the loss-of-function phyB-9 mutant

177 Using a recombinant virus-expression system in tissue culture and in mice, we demo

178 Using a linked conditional deletion/re-expression system in vivo, we demonstrated that re-expre

179 Using a heterologous expression system in which human PTEN-GFP is expressed i

180 enzyme, we used a cell-free wheat germ-based expression system in which mRNA encoding FERONIA was co-

181 g that is also used to induce the LexPR gene expression system in zebrafish and the gene-switch regul

182 r the existence of desiccation-specific gene expression systems in P. vanderplanki.

183 xpression of various AMPs using a CaM-fusion expression system, including melittin, fowlicidin-1, tri

184 inant spike proteins produced from different expression systems, including human embryonic kidney (HE

185 The capacity of different expression systems, including pseudoviral particles and

186 of mGluR2 in recombinant and native receptor expression systems, increasing the affinity of glutamate

187 tic dissection in situ and in a heterologous expression system indicate that this acid efflux is medi

188 Our cell-based transient expression system is fast and easy to set up and generat

189 iated iron transport in a yeast heterologous expression system is saturable (Km approximately 14.7 mu

190 low level of Nav1.9 currents in heterologous expression systems is not understood.

191 This shortcoming of bacterial expression systems is particularly detrimental for the f

192 However, in a heterologous expression system, it acts as a nucleotide antiporter an

193 However, when complex expression systems like hairy roots are used for product

194 In a heterologous expression system, Mel activates the human BK channel (h

195 Cell-free protein expression systems monitored the impact of Zt6 protein o

196 In each expression system, mutation of cysteines in GPIHBP1's Ly

197 This study establishes the recombinant expression system of MAF-1 (Musca domestica antifungal p

198 a phosphoproteomic screen using an inducible expression system of the bacterial effector avrRpt2 in A

199 An E. coli expression system offers a mean for rapid, high yield an

200 Here, we describe an optogenetic gene expression system optimized for use in zebrafish.

201 he proteins to be produced are toxic for the expression system or show unusual features that cannot b

202 ave relied on cultured neurons, heterologous expression systems, or membrane fusion assays in vitro,

203 In a heterologous expression system, OR51E2 trafficked readily to the cell

204 bition by Gbetagamma protein in heterologous expression systems, primary neurons and in vivo, and sug

205 A1 of living rats by means of the lentiviral expression system prior to induction of ischemia afforde

206 When tethered to RNAs in heterologous expression systems, RDE-3 can add long stretches of alte

207 Irrespective of the expression system, receptors resulting from combining th

208 perimental techniques, including conditional expression systems, recyclable markers, RNA interference

209 This cshRNA expression system reduces the coding space required for

210 of cmo-miR156, using a virus-based microRNA expression system, restores the early flowering phenotyp

211 dard RM 8670 derived from a murine cell line expression system resulted in detection of 60 mouse HCPs

212 ative Kir3.1) in different heterologous cell expression systems resulted in increased GIRK currents (

213 as between Rpg1b and Rpg1r using a transient expression system revealed that AvrB versus AvrRpm1 spec

214 Experiments with heterologous expression systems show that some of the identified Slo1

215 Further analysis using an inducible expression system showed that BAK1 accumulation quickly

216 cterization of duck Piezo2 in a heterologous expression system shows that duck Piezo2 is functionally

217 Binary expression systems such as GAL4/UAS, LexA/LexAop and QF/

218 e glycans on gp120 was reproduced across all expression systems, supporting this as an intrinsic prop

219 In a heterologous expression system, TbAAT10-1 is selective for ornithine,

220 In a heterologous expression system, TDAG8 potentiated the acid-induced ca

221 racterization of a novel tunable recombinant expression system, termed RiboTite, which operates at bo

222 We here present a novel FimH-based expression system that enables display of fully function

223 A conditional gene expression system that is fast-acting, is tunable and ac

224 Isolated protoplasts serve as a transient expression system that is highly representative of stabl

225 The Q-system is a binary expression system that works well across species.

226 Here, we describe a bacterial co-expression system that yields milligram quantities of th

227 It appears therefore that in the oocyte expression system the introduced cysteine residues in ma

228 in the expression of hClC-Kb in heterologous expression systems, the functional consequences of mutan

229 In heterologous expression systems, these subunits avidly coassemble and

230 In the context of the Xenopus laevis oocyte expression system, this technique yields ion channels wi

231 etically defined cell models and conditional expression systems, this response was shown to be Myc sp

232 loroplasts communicate with the nuclear gene expression system through a chloroplast unfolded protein

233 The Tornado expression system thus markedly enhances the utility of

234 ansposon and a Tet-On 3G drug-inducible gene expression system to achieve versatile inducible gene ex

235 crease the sensitivity of the virulence gene expression system to certain sugars that regulate it.

236 We took advantage of the Xenopus laevis expression system to determine the individual characteri

237 Our study utilizes an expression system to dissect the biophysical properties

238 ve of this study was to develop an efficient expression system to enable large-scale production of hi

239 is is the first report using the Tetrahymena expression system to generate C. irritans i-antigens and

240 their splice variants, in the same cellular expression system to investigate and compare their elect

241 to palivizumab efficacy using a plant-based expression system to produce palivizumab N-glycan struct

242 s to assess the suitability of a baculovirus expression system to produce recombinant RVFV nucleoprot

243 (2+) (TRIC) in Drosophila that uses a binary expression system to report Ca(2+)-dependent interaction

244 othesis, we used a diphtheria toxin receptor expression system to selectively ablate these cells from

245 engineered a Cre recombinase-dependent gene expression system to selectively express alpha4 nAChR su

246 In this study we utilized the Xenopus oocyte expression system to shed light on how CFTR channel acti

247 Here we used an in vitro expression system to study the insertion capacity of hyd

248 Here we used two different functional expression systems to characterize three Cx43 mutations

249 esonance energy transfer, and mammalian cell expression systems to clarify the effects of four common

250 e-based transport assays in two heterologous expression systems to establish a broad substrate recogn

251 Using an improved triple gRNA expression system (TRISPR [triple gRNA expression constr

252 d adult Drosophila Using the tissue-specific expression system (UAS)-GAL4, we express Caspase-LOV spe

253 ge of cellular phenotypes, yet most cellular expression systems use heterologous cDNA-based vectors w

254 se of NA and M2, clustering depends upon the expression system used.

255 SD-associated SCN2A variants in heterologous expression systems using whole-cell voltage-clamp electr

256 creening was conducted in the Xenopus oocyte expression system, using quantitative swelling and two-e

257 n in specific cell populations has made gene expression systems valuable tools in developmental biolo

258 ved with the combinatorial promoter stacking expression system was stable in multiple vegetative prop

259 A heterologous Lactococcus lactis expression system was used to express SdrF and its ligan

260 e outer hair cells (OHCs) and a heterologous expression system, we also found that this calcium-oblig

261 ng combinations of components in a bacterial expression system, we demonstrate noncatalytic BADCs fac

262 Using a recombinant expression system, we demonstrate that CO inhibits peak

263 Using a doxycycline (DOX)-regulated NS1 expression system, we demonstrate that DOX-induced NS1 i

264 sing an Escherichia coli heterologous sterol expression system, we demonstrate that substitution of f

265 Using an inducible expression system, we discovered that age-onset phenotyp

266 Utilizing a doxycycline-controlled SLUG expression system, we found that high expression of the

267 a regulatable (doxycycline-repressible) gene expression system, we found that the pathogenic PPA2 mut

268 Using a binary gene expression system, we generated transgenic mice expressi

269 Using a recombinant expression system, we have studied homogeneous samples o

270 otypical bitter substances in a heterologous expression system, we identified cognate compounds for 2

271 Through an inducible Twist2 expression system, we identified Twist2 as a reversible

272 Using an inducible expression system, we observed that this ELANE mutation

273 Using a lysozyme-M-cre (LysMcre) expression system, we show that deletion of autophagy-re

274 Using a heterologous NHE6-deficient cell expression system, we show that the biochemical, catalyt

275 Using a doxycycline-inducible RGN expression system, we showed that ectopic expression of

276 Using knockdown, knockout, and ectopic expression systems, we demonstrate the anti-HSV-1 activi

277 esponsive modification of plasmid-based gene expression systems, we have demonstrated optochemical ac

278 onal readouts in heterologous and endogenous expression systems, we identified the UDP receptor P2Y6

279 miR-449b through a doxycycline (dox) induced expression system were used as nuclear donor cells for S

280 nd a plant (Nicotiana benthamiana) transient expression system were used to test the ability of Arabi

281 Darby canine kidney (MDCK), and insect (Sf9) expression systems were examined.

282 Recombinant expression systems were generated for the bacterial ALDH

283 Transient expression systems were used to characterize Zt6, and mu

284 ed calcium (Ca(2+)) channels in heterologous expression systems were used to determine the modulation

285 Mx1-cre and Vav-cre expression systems were used to specifically target Rapt

286 forms, and among studies, different cellular expression systems were used.

287 of which 11 were expressed in more than one expression system, were determined in sera from 347 hors

288 pled to SpyTag expressed well in a mammalian expression system when an N-linked glycan was added to t

289 ressed using the baculovirus/Sf9 insect cell expression system, whereas others have been recalcitrant

290 ld and purity of HBc particles in an E. coli-expression system, which are fully characterised and sui

291 -length PfMyoA with the baculovirus/Sf9 cell expression system, which required a UCS (UNC-45/CRO1/She

292 classes using different recombinant protein expression systems, which were then evaluated in several

293 Furthermore, this plant expression system will fuel the development of helminth

294 function, we screened an AIRE-dependent gene-expression system with a genome-scale lentiviral shRNA l

295 analysed in an in vivo protoplast transient expression system with a novel mini-exon splicing report

296 nucleopolyhedrovirus (BmNPV)-based silkworm expression system with its innate signal peptide, bombyx

297 optimized pyrrolysyl-tRNA synthetase/tRNACUA expression system, with optimized tRNA expression for ma

298 This study presents an alternative expression system, without the need of a fusion protein

299 experiments using various heterologous cell expression systems (Xenopus oocytes, CHO cells, and rat

300 Our expression system yielded milligram quantities of pure r